Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061973.t1 | XP_003618953.1 | 93.6 | 236 | 7 | 1 | 1 | 236 | 1 | 228 | 2.10E-117 | 431.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061973.t1 | B7FJV5 | 93.6 | 236 | 7 | 1 | 1 | 236 | 1 | 228 | 1.5e-117 | 431.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050105 | MS.gene061973 | 0.805326 | 1.41E-49 | -1.69E-46 |
| MS.gene050508 | MS.gene061973 | 0.858193 | 9.75E-63 | -1.69E-46 |
| MS.gene051368 | MS.gene061973 | 0.819017 | 1.45E-52 | -1.69E-46 |
| MS.gene052333 | MS.gene061973 | 0.830432 | 2.95E-55 | -1.69E-46 |
| MS.gene055225 | MS.gene061973 | 0.836508 | 8.98E-57 | -1.69E-46 |
| MS.gene055701 | MS.gene061973 | 0.806143 | 9.49E-50 | -1.69E-46 |
| MS.gene057156 | MS.gene061973 | -0.816605 | 5.07E-52 | -1.69E-46 |
| MS.gene059710 | MS.gene061973 | 0.859868 | 3.07E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061973.t1 | MTR_6g029250 | 93.644 | 236 | 7 | 1 | 1 | 236 | 1 | 228 | 4.06e-163 | 450 |
| MS.gene061973.t1 | MTR_7g085790 | 48.462 | 260 | 97 | 9 | 1 | 233 | 1 | 250 | 6.19e-67 | 207 |
| MS.gene061973.t1 | MTR_7g085790 | 44.800 | 250 | 82 | 10 | 1 | 233 | 1 | 211 | 7.28e-55 | 175 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061973.t1 | AT3G03870 | 37.545 | 277 | 116 | 15 | 1 | 233 | 1 | 264 | 2.51e-31 | 116 |
| MS.gene061973.t1 | AT5G18130 | 42.857 | 175 | 58 | 8 | 91 | 233 | 114 | 278 | 7.50e-31 | 115 |
| MS.gene061973.t1 | AT3G03870 | 33.058 | 242 | 106 | 14 | 1 | 199 | 1 | 229 | 1.63e-14 | 70.9 |
| MS.gene061973.t1 | AT5G18130 | 36.364 | 143 | 50 | 7 | 91 | 202 | 114 | 246 | 3.75e-14 | 70.1 |
Find 33 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAATTGCTTCATTCAATT+TGG | 0.207751 | 6.2:-20659648 | None:intergenic |
| GTTATGTGAGCTCTTGTTAT+AGG | 0.220981 | 6.2:-20659686 | None:intergenic |
| TCTTTCTCAATAATCATTCT+TGG | 0.230298 | 6.2:-20658596 | None:intergenic |
| AAGATTAAGGAAAATGAAAT+TGG | 0.238321 | 6.2:+20658518 | MS.gene061973:CDS |
| GTTGGTGCTATTTGTACTTT+TGG | 0.274032 | 6.2:+20658704 | MS.gene061973:CDS |
| ATTATTGAGAAAGATGATTT+TGG | 0.285258 | 6.2:+20658605 | MS.gene061973:CDS |
| CCTTGTCATCAGCATAGATT+TGG | 0.343870 | 6.2:-20658808 | None:intergenic |
| TTACCAAGTTTGAGGTCTAA+TGG | 0.382258 | 6.2:-20658407 | None:intergenic |
| TAACACCTCAAATGATGTTA+TGG | 0.383015 | 6.2:+20658427 | MS.gene061973:CDS |
| GAAGCAATTTCTGTTGTAAG+AGG | 0.417052 | 6.2:+20659659 | MS.gene061973:CDS |
| GGTCTAATGGAATTTGTAAA+GGG | 0.426783 | 6.2:-20658394 | None:intergenic |
| TTATGGAAGTGGAGTCTTAG+TGG | 0.435150 | 6.2:+20658680 | MS.gene061973:CDS |
| TGGGAAGAAGAGAATAATAG+TGG | 0.445840 | 6.2:+20658650 | MS.gene061973:CDS |
| TTGAGGTGTTACCAAGTTTG+AGG | 0.452496 | 6.2:-20658415 | None:intergenic |
| AGGTCTAATGGAATTTGTAA+AGG | 0.463571 | 6.2:-20658395 | None:intergenic |
| TTGAACCATAACATCATTTG+AGG | 0.471724 | 6.2:-20658432 | None:intergenic |
| TCTTTGCTACTACTTGACTT+TGG | 0.472978 | 6.2:-20658560 | None:intergenic |
| TGGAGATGGATCTTGATGAA+TGG | 0.483700 | 6.2:+20658234 | None:intergenic |
| GAATTTGTAAAGGGACAAGT+TGG | 0.486114 | 6.2:-20658385 | None:intergenic |
| GGAGATGGATCTTGATGAAT+GGG | 0.498676 | 6.2:+20658235 | None:intergenic |
| TTCAGAAGGATCAGAAGAAT+AGG | 0.499980 | 6.2:+20658783 | MS.gene061973:CDS |
| ATTCCATTAGACCTCAAACT+TGG | 0.502699 | 6.2:+20658404 | MS.gene061973:CDS |
| CAAAGAAGAGGATTCAAGTT+GGG | 0.511674 | 6.2:+20658631 | MS.gene061973:CDS |
| TATATTGATTTCAATGAAGA+TGG | 0.511704 | 6.2:+20658278 | MS.gene061973:CDS |
| TGTTCTTTGGAAGCCAACAT+AGG | 0.514724 | 6.2:+20658753 | MS.gene061973:CDS |
| AAGTGGAGTCTTAGTGGTGT+TGG | 0.575334 | 6.2:+20658686 | MS.gene061973:CDS |
| ATTTCAGAGGATTAAGCAAG+TGG | 0.577509 | 6.2:+20659613 | MS.gene061973:intron |
| ACAAAGAAGAGGATTCAAGT+TGG | 0.588072 | 6.2:+20658630 | MS.gene061973:CDS |
| CCAAATCTATGCTGATGACA+AGG | 0.589218 | 6.2:+20658808 | MS.gene061973:CDS |
| AGAGCTCACATAACAGTTGG+TGG | 0.589518 | 6.2:+20659695 | MS.gene061973:CDS |
| TGGGAATCAATCTCTAGTGA+TGG | 0.593054 | 6.2:+20658254 | MS.gene061973:CDS |
| ACAAGAGCTCACATAACAGT+TGG | 0.595311 | 6.2:+20659692 | MS.gene061973:CDS |
| ATCAGAAAGTGCTTTAGACA+TGG | 0.600275 | 6.2:+20658328 | MS.gene061973:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGATTAGTAGAAAAAATA+TGG | - | chr6.2:20659408-20659427 | None:intergenic | 15.0% |
| !!! | AAAAAAAAGTATTAACTTTG+TGG | + | chr6.2:20659534-20659553 | MS.gene061973:intron | 15.0% |
| !!! | ATAATAGTGGTTTTAATTTA+TGG | + | chr6.2:20658663-20658682 | MS.gene061973:CDS | 15.0% |
| !! | AAGATTAAGGAAAATGAAAT+TGG | + | chr6.2:20658518-20658537 | MS.gene061973:CDS | 20.0% |
| !! | CATAATCAATAAGTCAAAAA+CGG | - | chr6.2:20659333-20659352 | None:intergenic | 20.0% |
| !! | TAAACCATTTATACTAAATC+AGG | - | chr6.2:20658963-20658982 | None:intergenic | 20.0% |
| !!! | ACAAGTTTTCTTTAAGATTA+AGG | + | chr6.2:20658505-20658524 | MS.gene061973:CDS | 20.0% |
| !!! | ATTATTGAGAAAGATGATTT+TGG | + | chr6.2:20658605-20658624 | MS.gene061973:CDS | 20.0% |
| !!! | TAGCAATACTTTTTTTTTGA+GGG | + | chr6.2:20659099-20659118 | MS.gene061973:intron | 20.0% |
| !!! | TATATTGATTTCAATGAAGA+TGG | + | chr6.2:20658278-20658297 | MS.gene061973:CDS | 20.0% |
| !!! | TTTTAGTCATTATGTTGTTA+AGG | + | chr6.2:20659134-20659153 | MS.gene061973:intron | 20.0% |
| ! | AAAAAAAGTTTCCTAGGAAA+AGG | + | chr6.2:20658305-20658324 | MS.gene061973:CDS | 25.0% |
| ! | AGAAATTGCTTCATTCAATT+TGG | - | chr6.2:20659651-20659670 | None:intergenic | 25.0% |
| ! | AGAACCTGATTTAGTATAAA+TGG | + | chr6.2:20658956-20658975 | MS.gene061973:intron | 25.0% |
| ! | TCTTTCTCAATAATCATTCT+TGG | - | chr6.2:20658599-20658618 | None:intergenic | 25.0% |
| ! | TGATATGAACTCTTTATAGT+TGG | + | chr6.2:20659206-20659225 | MS.gene061973:intron | 25.0% |
| ! | TGTTAATGACTAATGTTTGT+CGG | + | chr6.2:20658933-20658952 | MS.gene061973:intron | 25.0% |
| ! | TTCATATCAATCAACTAACA+AGG | - | chr6.2:20659195-20659214 | None:intergenic | 25.0% |
| !!! | GTAGCAATACTTTTTTTTTG+AGG | + | chr6.2:20659098-20659117 | MS.gene061973:intron | 25.0% |
| !!! | TCTATTTGTGTTTTGTTCTT+TGG | + | chr6.2:20658740-20658759 | MS.gene061973:CDS | 25.0% |
| ACATAGGAAAAAACTTCAGA+AGG | + | chr6.2:20658769-20658788 | MS.gene061973:CDS | 30.0% | |
| AGGTCTAATGGAATTTGTAA+AGG | - | chr6.2:20658398-20658417 | None:intergenic | 30.0% | |
| GACAAGGTTAGTAAATTTCT+TGG | + | chr6.2:20658824-20658843 | MS.gene061973:intron | 30.0% | |
| GGTCTAATGGAATTTGTAAA+GGG | - | chr6.2:20658397-20658416 | None:intergenic | 30.0% | |
| TAACACCTCAAATGATGTTA+TGG | + | chr6.2:20658427-20658446 | MS.gene061973:CDS | 30.0% | |
| TTGAACCATAACATCATTTG+AGG | - | chr6.2:20658435-20658454 | None:intergenic | 30.0% | |
| TTTATAACAACTCTAACCCT+TGG | + | chr6.2:20659585-20659604 | MS.gene061973:intron | 30.0% | |
| !! | ATCCTCTGAAATTAAAACCA+AGG | - | chr6.2:20659605-20659624 | None:intergenic | 30.0% |
| !! | TCCTCTGAAATTAAAACCAA+GGG | - | chr6.2:20659604-20659623 | None:intergenic | 30.0% |
| !!! | ACAAGTTGGTTTTGTTTTCT+TGG | - | chr6.2:20658374-20658393 | None:intergenic | 30.0% |
| !!! | GTGGTTTTAATTTATGGAAG+TGG | + | chr6.2:20658669-20658688 | MS.gene061973:CDS | 30.0% |
| !!! | TCTGAAGTTTTTTCCTATGT+TGG | - | chr6.2:20658769-20658788 | None:intergenic | 30.0% |
| ACAAAGAAGAGGATTCAAGT+TGG | + | chr6.2:20658630-20658649 | MS.gene061973:CDS | 35.0% | |
| ATTCCATTAGACCTCAAACT+TGG | + | chr6.2:20658404-20658423 | MS.gene061973:CDS | 35.0% | |
| ATTTCAGAGGATTAAGCAAG+TGG | + | chr6.2:20659613-20659632 | MS.gene061973:intron | 35.0% | |
| CTTTATAGTTGGAGAAACTG+TGG | + | chr6.2:20659217-20659236 | MS.gene061973:intron | 35.0% | |
| GAAGCAATTTCTGTTGTAAG+AGG | + | chr6.2:20659659-20659678 | MS.gene061973:CDS | 35.0% | |
| GAATTTGTAAAGGGACAAGT+TGG | - | chr6.2:20658388-20658407 | None:intergenic | 35.0% | |
| GATAAGAGACTGAATCTAAG+TGG | + | chr6.2:20659430-20659449 | MS.gene061973:intron | 35.0% | |
| GGTGAGAAAAAAAGTTTCCT+AGG | + | chr6.2:20658299-20658318 | MS.gene061973:CDS | 35.0% | |
| TCTTTGCTACTACTTGACTT+TGG | - | chr6.2:20658563-20658582 | None:intergenic | 35.0% | |
| TGGGAAGAAGAGAATAATAG+TGG | + | chr6.2:20658650-20658669 | MS.gene061973:CDS | 35.0% | |
| TTCAGAAGGATCAGAAGAAT+AGG | + | chr6.2:20658783-20658802 | MS.gene061973:CDS | 35.0% | |
| TTGTTAAGGCTGCAATTACA+TGG | + | chr6.2:20659148-20659167 | MS.gene061973:intron | 35.0% | |
| ! | CAAAGAAGAGGATTCAAGTT+GGG | + | chr6.2:20658631-20658650 | MS.gene061973:CDS | 35.0% |
| ! | CTGCAATTACATGGTAGTTT+TGG | + | chr6.2:20659157-20659176 | MS.gene061973:intron | 35.0% |
| ! | CTTGTGACACATTTTCTTCT+TGG | - | chr6.2:20658490-20658509 | None:intergenic | 35.0% |
| ! | GTTATGTGAGCTCTTGTTAT+AGG | - | chr6.2:20659689-20659708 | None:intergenic | 35.0% |
| ! | TTACCAAGTTTGAGGTCTAA+TGG | - | chr6.2:20658410-20658429 | None:intergenic | 35.0% |
| !! | ATCAGAAAGTGCTTTAGACA+TGG | + | chr6.2:20658328-20658347 | MS.gene061973:CDS | 35.0% |
| !! | TGATTTTGGTGACAAAGAAG+AGG | + | chr6.2:20658619-20658638 | MS.gene061973:CDS | 35.0% |
| !!! | ACCCTTGGTTTTAATTTCAG+AGG | + | chr6.2:20659600-20659619 | MS.gene061973:intron | 35.0% |
| !!! | AGTTGGTTTTGTTTTCTTGG+TGG | - | chr6.2:20658371-20658390 | None:intergenic | 35.0% |
| !!! | GTTGGTGCTATTTGTACTTT+TGG | + | chr6.2:20658704-20658723 | MS.gene061973:CDS | 35.0% |
| !!! | TTTTGTTTTCTTGGTGGTGA+AGG | - | chr6.2:20658365-20658384 | None:intergenic | 35.0% |
| ACAAGAGCTCACATAACAGT+TGG | + | chr6.2:20659692-20659711 | MS.gene061973:CDS | 40.0% | |
| CCAAATCTATGCTGATGACA+AGG | + | chr6.2:20658808-20658827 | MS.gene061973:CDS | 40.0% | |
| CCTTGTCATCAGCATAGATT+TGG | - | chr6.2:20658811-20658830 | None:intergenic | 40.0% | |
| TGGGAATCAATCTCTAGTGA+TGG | + | chr6.2:20658254-20658273 | MS.gene061973:CDS | 40.0% | |
| TTATGGAAGTGGAGTCTTAG+TGG | + | chr6.2:20658680-20658699 | MS.gene061973:CDS | 40.0% | |
| ! | CACTTTCTGATCCTTTTCCT+AGG | - | chr6.2:20658319-20658338 | None:intergenic | 40.0% |
| ! | TGTTCTTTGGAAGCCAACAT+AGG | + | chr6.2:20658753-20658772 | MS.gene061973:CDS | 40.0% |
| ! | TTGAGGTGTTACCAAGTTTG+AGG | - | chr6.2:20658418-20658437 | None:intergenic | 40.0% |
| AAGTGGAGTCTTAGTGGTGT+TGG | + | chr6.2:20658686-20658705 | MS.gene061973:CDS | 45.0% | |
| AGAGCTCACATAACAGTTGG+TGG | + | chr6.2:20659695-20659714 | MS.gene061973:CDS | 45.0% | |
| GACTGAATCTAAGTGGATCG+TGG | + | chr6.2:20659437-20659456 | MS.gene061973:intron | 45.0% | |
| !!! | AATCATATATATATATTTTT+TGG | + | chr6.2:20658856-20658875 | MS.gene061973:intron | 5.0% |
| CTAAGTGGATCGTGGCTGCA+AGG | + | chr6.2:20659445-20659464 | MS.gene061973:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 20658239 | 20659739 | 20658239 | ID=MS.gene061973 |
| chr6.2 | mRNA | 20658239 | 20659739 | 20658239 | ID=MS.gene061973.t1;Parent=MS.gene061973 |
| chr6.2 | exon | 20658239 | 20658829 | 20658239 | ID=MS.gene061973.t1.exon1;Parent=MS.gene061973.t1 |
| chr6.2 | CDS | 20658239 | 20658829 | 20658239 | ID=cds.MS.gene061973.t1;Parent=MS.gene061973.t1 |
| chr6.2 | exon | 20659620 | 20659739 | 20659620 | ID=MS.gene061973.t1.exon2;Parent=MS.gene061973.t1 |
| chr6.2 | CDS | 20659620 | 20659739 | 20659620 | ID=cds.MS.gene061973.t1;Parent=MS.gene061973.t1 |
| Gene Sequence |
| Protein sequence |
