AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051368


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051368.t1 MTR_7g103900 95.806 310 9 2 1 306 1 310 0.0 603
MS.gene051368.t1 MTR_7g103900 94.760 229 11 1 1 228 1 229 1.10e-157 439
MS.gene051368.t1 MTR_4g055230 52.090 311 137 10 1 305 1 305 1.28e-95 285
MS.gene051368.t1 MTR_2g084965 49.838 309 140 9 4 304 6 307 3.02e-83 253
MS.gene051368.t1 MTR_7g095100 35.357 280 154 9 1 273 1 260 6.35e-49 165
MS.gene051368.t1 MTR_7g114790 35.870 276 153 7 1 273 1 255 1.36e-48 164
MS.gene051368.t1 MTR_1g081260 32.919 322 179 9 1 305 1 302 1.08e-46 159
MS.gene051368.t1 MTR_3g106250 30.000 230 131 7 56 272 48 260 8.45e-30 116
MS.gene051368.t1 MTR_5g069570 29.832 238 155 7 37 272 34 261 2.38e-29 114
MS.gene051368.t1 MTR_3g076610 29.787 235 141 4 56 272 46 274 7.70e-29 113
MS.gene051368.t1 MTR_4g104760 28.700 223 150 2 56 272 47 266 8.87e-28 110
MS.gene051368.t1 MTR_3g082430 30.894 246 155 9 31 272 10 244 2.26e-27 108
MS.gene051368.t1 MTR_1g013100 29.323 266 159 10 20 272 11 260 2.94e-27 108
MS.gene051368.t1 MTR_5g096570 27.854 219 145 4 57 272 1208 1416 3.76e-25 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051368.t1 AT2G27770 43.077 325 158 11 1 304 1 319 9.44e-74 229
MS.gene051368.t1 AT2G36470 37.681 345 155 9 1 304 1 326 1.86e-63 203
MS.gene051368.t1 AT3G04860 33.981 309 179 10 1 305 1 288 1.11e-48 164
MS.gene051368.t1 AT5G28150 33.882 304 176 9 1 300 1 283 1.31e-46 159
MS.gene051368.t1 AT5G11000 29.474 285 148 10 38 293 33 293 4.13e-33 125
MS.gene051368.t1 AT4G12690 29.218 243 160 6 35 276 22 253 6.61e-32 120
MS.gene051368.t1 AT4G12690 29.218 243 160 6 35 276 22 253 6.61e-32 120
MS.gene051368.t1 AT5G48270 27.984 243 164 5 33 272 35 269 4.14e-29 114
MS.gene051368.t1 AT2G04220 29.333 225 150 5 35 258 26 242 4.67e-28 110
MS.gene051368.t1 AT3G13229 28.235 255 156 10 38 280 6 245 5.38e-25 102
MS.gene051368.t1 AT2G25200 25.347 288 170 9 4 272 14 275 2.95e-22 95.5

Find 42 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACATGTTATGTTTGTTTA+TGG 0.113859 7.3:+10264106 MS.gene051368:CDS
CCATGATGCGAAATTCTTAA+TGG 0.156239 7.3:+10263956 MS.gene051368:CDS
GGATAAGAAGATGGTGTTTA+AGG 0.193714 7.3:+10264127 MS.gene051368:CDS
TTGCAGAAACAAAAGGAAAT+TGG 0.201357 7.3:+10264335 MS.gene051368:CDS
ATTGAAGGGTTATTGAGTTT+TGG 0.288969 7.3:-10263558 None:intergenic
GAAGGACCTGAACCGGTTAA+TGG 0.294367 7.3:+10263843 MS.gene051368:CDS
CTTTGTTCTGTTTCAACAAC+AGG 0.307651 7.3:-10263749 None:intergenic
GCCTTGACCAATGAGTTTCT+TGG 0.347604 7.3:-10263658 None:intergenic
TGATGTGGGATTTGCATGAT+TGG 0.365014 7.3:+10264216 MS.gene051368:CDS
TCTGAATTGGGTTTATTCCT+TGG 0.378159 7.3:+10263891 MS.gene051368:CDS
TAAAACCCATTAACCGGTTC+AGG 0.396705 7.3:-10263849 None:intergenic
GTGGTTTGAATAGTAGATTA+TGG 0.398722 7.3:+10264297 MS.gene051368:CDS
AAGGACCTGAACCGGTTAAT+GGG 0.411080 7.3:+10263844 MS.gene051368:CDS
AGAAGAATCGTTGAAGAACT+TGG 0.436270 7.3:+10263920 MS.gene051368:CDS
CCATTAAGAATTTCGCATCA+TGG 0.449935 7.3:-10263956 None:intergenic
ATATAAATCAGATACTCTAA+TGG 0.450641 7.3:-10263508 None:intergenic
AATTTCTCATCACACTAACT+TGG 0.457736 7.3:+10263634 MS.gene051368:CDS
TTTGGAATGATTGAATTTGA+AGG 0.474609 7.3:-10263540 None:intergenic
ATGACGTAAAACCCATTAAC+CGG 0.480234 7.3:-10263855 None:intergenic
GAAGAATTTGCAGAAACAAA+AGG 0.481804 7.3:+10264328 MS.gene051368:CDS
GGATTCTCTTTGTTGATATG+TGG 0.485386 7.3:+10264356 MS.gene051368:CDS
GTATGATGAAGGACCTGAAC+CGG 0.486574 7.3:+10263836 MS.gene051368:CDS
ACTTGGACCAAGAAACTCAT+TGG 0.498976 7.3:+10263651 MS.gene051368:CDS
ACGATTCTTCTTTGTCTCCA+AGG 0.499539 7.3:-10263908 None:intergenic
TTTGTTTATGGATAAGAAGA+TGG 0.500940 7.3:+10264118 MS.gene051368:CDS
TTTGAATAGTAGATTATGGT+TGG 0.508048 7.3:+10264301 MS.gene051368:CDS
AAAGATAGAATCAATCAGGC+AGG 0.510778 7.3:-10264389 None:intergenic
TTTGGTTGTGGATATGATGT+GGG 0.513802 7.3:+10264202 MS.gene051368:CDS
AAGTGTGGAGTAGAAGAAGA+AGG 0.514583 7.3:+10264068 MS.gene051368:CDS
GAAACAGAATCAATATTACT+AGG 0.527556 7.3:-10264248 None:intergenic
GTTTGGTTGTGGATATGATG+TGG 0.546002 7.3:+10264201 MS.gene051368:CDS
CACGATATATTGATTAAGTG+TGG 0.553076 7.3:+10264053 MS.gene051368:CDS
TTCTGTTTCAACAACAGGGG+AGG 0.562675 7.3:-10263744 None:intergenic
TCTGATGCTAAGTATGATGA+AGG 0.568147 7.3:+10263825 MS.gene051368:CDS
ACCAAGAAACTCATTGGTCA+AGG 0.578820 7.3:+10263657 MS.gene051368:CDS
TTCAAGTGTTTATGCAACAA+AGG 0.579019 7.3:+10264004 MS.gene051368:CDS
TTAAGGTGAAGAGATTGAAG+TGG 0.600677 7.3:+10264144 MS.gene051368:CDS
TTTATGTTTAGGACAAGGAG+TGG 0.606906 7.3:+10264278 MS.gene051368:CDS
TTTGTTCTGTTTCAACAACA+GGG 0.613037 7.3:-10263748 None:intergenic
AAGATAGAATCAATCAGGCA+GGG 0.625244 7.3:-10264388 None:intergenic
TTGTTCTGTTTCAACAACAG+GGG 0.641999 7.3:-10263747 None:intergenic
CTGTTGTTGAAACAGAACAA+AGG 0.691667 7.3:+10263750 MS.gene051368:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAAATCAGATACTCTAA+TGG - chr7.3:10263511-10263530 None:intergenic 20.0%
!!! TAACTGAATTTTGAATTGAA+GGG - chr7.3:10263575-10263594 None:intergenic 20.0%
! GAAACAGAATCAATATTACT+AGG - chr7.3:10264251-10264270 None:intergenic 25.0%
! TCACATGTTATGTTTGTTTA+TGG + chr7.3:10264106-10264125 MS.gene051368:CDS 25.0%
! TTTGAATAGTAGATTATGGT+TGG + chr7.3:10264301-10264320 MS.gene051368:CDS 25.0%
! TTTGTTTATGGATAAGAAGA+TGG + chr7.3:10264118-10264137 MS.gene051368:CDS 25.0%
!! AATCAAACAATTTTTGTGGA+TGG + chr7.3:10264179-10264198 MS.gene051368:CDS 25.0%
!! ATTTCGAAGTCAAAGTTTTA+TGG + chr7.3:10263796-10263815 MS.gene051368:CDS 25.0%
!! CATGTTTTCTCAAAATTCAA+TGG + chr7.3:10263717-10263736 MS.gene051368:CDS 25.0%
!! GTAACTGAATTTTGAATTGA+AGG - chr7.3:10263576-10263595 None:intergenic 25.0%
!! TTTGGAATGATTGAATTTGA+AGG - chr7.3:10263543-10263562 None:intergenic 25.0%
!!! CATTGAATTTTGAGAAAACA+TGG - chr7.3:10263719-10263738 None:intergenic 25.0%
!!! TTTCGAAGTCAAAGTTTTAT+GGG + chr7.3:10263797-10263816 MS.gene051368:CDS 25.0%
!!! TTTCTGCTGTTTTTATGTTT+AGG + chr7.3:10264267-10264286 MS.gene051368:CDS 25.0%
AATTTCTCATCACACTAACT+TGG + chr7.3:10263634-10263653 MS.gene051368:CDS 30.0%
GAAGAATTTGCAGAAACAAA+AGG + chr7.3:10264328-10264347 MS.gene051368:CDS 30.0%
GTGGTTTGAATAGTAGATTA+TGG + chr7.3:10264297-10264316 MS.gene051368:CDS 30.0%
TTCAAGTGTTTATGCAACAA+AGG + chr7.3:10264004-10264023 MS.gene051368:CDS 30.0%
TTGCAGAAACAAAAGGAAAT+TGG + chr7.3:10264335-10264354 MS.gene051368:CDS 30.0%
TTTGTTCTGTTTCAACAACA+GGG - chr7.3:10263751-10263770 None:intergenic 30.0%
! AACAATTTTTGTGGATGGTT+TGG + chr7.3:10264184-10264203 MS.gene051368:CDS 30.0%
! ATTGAAGGGTTATTGAGTTT+TGG - chr7.3:10263561-10263580 None:intergenic 30.0%
! CACGATATATTGATTAAGTG+TGG + chr7.3:10264053-10264072 MS.gene051368:CDS 30.0%
! TCAAGAAGTGAAAGATTTTC+TGG + chr7.3:10263981-10264000 MS.gene051368:CDS 30.0%
! TGGGAATCAAACAATTTTTG+TGG + chr7.3:10264175-10264194 MS.gene051368:CDS 30.0%
!!! CTGTTTTTATGTTTAGGACA+AGG + chr7.3:10264273-10264292 MS.gene051368:CDS 30.0%
AGAAGAATCGTTGAAGAACT+TGG + chr7.3:10263920-10263939 MS.gene051368:CDS 35.0%
ATGACGTAAAACCCATTAAC+CGG - chr7.3:10263858-10263877 None:intergenic 35.0%
CCATGATGCGAAATTCTTAA+TGG + chr7.3:10263956-10263975 MS.gene051368:CDS 35.0%
CCATTAAGAATTTCGCATCA+TGG - chr7.3:10263959-10263978 None:intergenic 35.0%
CTGTTGTTGAAACAGAACAA+AGG + chr7.3:10263750-10263769 MS.gene051368:CDS 35.0%
CTTTGTTCTGTTTCAACAAC+AGG - chr7.3:10263752-10263771 None:intergenic 35.0%
GGATAAGAAGATGGTGTTTA+AGG + chr7.3:10264127-10264146 MS.gene051368:CDS 35.0%
GGATTCTCTTTGTTGATATG+TGG + chr7.3:10264356-10264375 MS.gene051368:CDS 35.0%
TCTGAATTGGGTTTATTCCT+TGG + chr7.3:10263891-10263910 MS.gene051368:CDS 35.0%
TCTGATGCTAAGTATGATGA+AGG + chr7.3:10263825-10263844 MS.gene051368:CDS 35.0%
TTAAGGTGAAGAGATTGAAG+TGG + chr7.3:10264144-10264163 MS.gene051368:CDS 35.0%
TTGTTCTGTTTCAACAACAG+GGG - chr7.3:10263750-10263769 None:intergenic 35.0%
TTTGGTTGTGGATATGATGT+GGG + chr7.3:10264202-10264221 MS.gene051368:CDS 35.0%
! AGATTGAAGTGGAATTTTCG+TGG + chr7.3:10264155-10264174 MS.gene051368:CDS 35.0%
! GATTGAAGTGGAATTTTCGT+GGG + chr7.3:10264156-10264175 MS.gene051368:CDS 35.0%
! TTTATGTTTAGGACAAGGAG+TGG + chr7.3:10264278-10264297 MS.gene051368:CDS 35.0%
!! GTTTTGGTGAACTCTGAATT+GGG + chr7.3:10263879-10263898 MS.gene051368:CDS 35.0%
!!! AAGGCAAAGTTTTCTGAAAC+AGG + chr7.3:10264023-10264042 MS.gene051368:CDS 35.0%
AAGTGTGGAGTAGAAGAAGA+AGG + chr7.3:10264068-10264087 MS.gene051368:CDS 40.0%
ACCAAGAAACTCATTGGTCA+AGG + chr7.3:10263657-10263676 MS.gene051368:CDS 40.0%
ACGATTCTTCTTTGTCTCCA+AGG - chr7.3:10263911-10263930 None:intergenic 40.0%
ACTTGGACCAAGAAACTCAT+TGG + chr7.3:10263651-10263670 MS.gene051368:CDS 40.0%
GTTTGGTTGTGGATATGATG+TGG + chr7.3:10264201-10264220 MS.gene051368:CDS 40.0%
TGATGTGGGATTTGCATGAT+TGG + chr7.3:10264216-10264235 MS.gene051368:CDS 40.0%
! TAAAACCCATTAACCGGTTC+AGG - chr7.3:10263852-10263871 None:intergenic 40.0%
! TGGGTTTTACGTCATGGTTT+TGG + chr7.3:10263863-10263882 MS.gene051368:CDS 40.0%
!! GGTTTTGGTGAACTCTGAAT+TGG + chr7.3:10263878-10263897 MS.gene051368:CDS 40.0%
!!! GGTTAATGGGTTTTACGTCA+TGG + chr7.3:10263857-10263876 MS.gene051368:CDS 40.0%
!!! TTTTGTGGATGGTTTGGTTG+TGG + chr7.3:10264190-10264209 MS.gene051368:CDS 40.0%
AAGGACCTGAACCGGTTAAT+GGG + chr7.3:10263844-10263863 MS.gene051368:CDS 45.0%
GCCTTGACCAATGAGTTTCT+TGG - chr7.3:10263661-10263680 None:intergenic 45.0%
GTATGATGAAGGACCTGAAC+CGG + chr7.3:10263836-10263855 MS.gene051368:CDS 45.0%
TTCTGTTTCAACAACAGGGG+AGG - chr7.3:10263747-10263766 None:intergenic 45.0%
GAAGGACCTGAACCGGTTAA+TGG + chr7.3:10263843-10263862 MS.gene051368:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 10263474 10264397 10263474 ID=MS.gene051368
chr7.3 mRNA 10263474 10264397 10263474 ID=MS.gene051368.t1;Parent=MS.gene051368
chr7.3 exon 10263474 10264397 10263474 ID=MS.gene051368.t1.exon1;Parent=MS.gene051368.t1
chr7.3 CDS 10263474 10264397 10263474 ID=cds.MS.gene051368.t1;Parent=MS.gene051368.t1
Gene Sequence

>MS.gene051368

ATGAGAGGCATAACATCTTGTTACAATGAACATGCCATTAGAGTATCTGATTTATATTGTTCAAGACCTTCAAATTCAATCATTCCAAAACTCAATAACCCTTCAATTCAAAATTCAGTTACTTGCATATACAAACTCAACTTTCTATCAATTCAGCAACAATTTCTCATCACACTAACTTGGACCAAGAAACTCATTGGTCAAGGCTTCATCATAAACATCACTTCTGATTCTGATTATGTCCATGTTTTCTCAAAATTCAATGGAAATCCTCCCCTGTTGTTGAAACAGAACAAAGGCACTGATACGTTTCAATATCAAAATTTCGAAGTCAAAGTTTTATGGGATCTTTCTGATGCTAAGTATGATGAAGGACCTGAACCGGTTAATGGGTTTTACGTCATGGTTTTGGTGAACTCTGAATTGGGTTTATTCCTTGGAGACAAAGAAGAATCGTTGAAGAACTTGGAAGATAAGAAAAACCATGATGCGAAATTCTTAATGGTTTCAAGAAGTGAAAGATTTTCTGGTTCAAGTGTTTATGCAACAAAGGCAAAGTTTTCTGAAACAGGAATTTCACACGATATATTGATTAAGTGTGGAGTAGAAGAAGAAGGATCAACATCAAAGAGTCACATGTTATGTTTGTTTATGGATAAGAAGATGGTGTTTAAGGTGAAGAGATTGAAGTGGAATTTTCGTGGGAATCAAACAATTTTTGTGGATGGTTTGGTTGTGGATATGATGTGGGATTTGCATGATTGGATTTTCAATCCTAGTAATATTGATTCTGTTTCTGCTGTTTTTATGTTTAGGACAAGGAGTGGTTTGAATAGTAGATTATGGTTGGAAGAGAAGAATTTGCAGAAACAAAAGGAAATTGGATTCTCTTTGTTGATATGTGGATGTAAGAACCCTGCCTGA

Protein sequence

>MS.gene051368.t1

MRGITSCYNEHAIRVSDLYCSRPSNSIIPKLNNPSIQNSVTCIYKLNFLSIQQQFLITLTWTKKLIGQGFIINITSDSDYVHVFSKFNGNPPLLLKQNKGTDTFQYQNFEVKVLWDLSDAKYDEGPEPVNGFYVMVLVNSELGLFLGDKEESLKNLEDKKNHDAKFLMVSRSERFSGSSVYATKAKFSETGISHDILIKCGVEEEGSTSKSHMLCLFMDKKMVFKVKRLKWNFRGNQTIFVDGLVVDMMWDLHDWIFNPSNIDSVSAVFMFRTRSGLNSRLWLEEKNLQKQKEIGFSLLICGCKNPA