Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062119.t1 | XP_013463630.1 | 96.2 | 79 | 3 | 0 | 1 | 79 | 1 | 79 | 3.90E-32 | 147.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062119.t1 | O48713 | 67.5 | 80 | 25 | 1 | 1 | 79 | 1 | 80 | 1.9e-19 | 96.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062119.t1 | A0A072VHN3 | 96.2 | 79 | 3 | 0 | 1 | 79 | 1 | 79 | 2.8e-32 | 147.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057869 | MS.gene062119 | 0.815814 | 7.62E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene062119 | 0.808426 | 3.12E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062119.t1 | MTR_2g044200 | 96.203 | 79 | 3 | 0 | 1 | 79 | 1 | 79 | 9.55e-46 | 154 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062119.t1 | AT2G26460 | 67.500 | 80 | 25 | 1 | 1 | 79 | 1 | 80 | 1.30e-26 | 101 |
Find 13 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACTTACTTGCGGCAAAT+TGG | 0.304429 | 2.3:+45778304 | None:intergenic |
| AGTTCAGTTTGTTCATAATC+AGG | 0.323683 | 2.3:+45778105 | None:intergenic |
| CTCCTGGTACCGTTGATATC+AGG | 0.375252 | 2.3:-45778062 | MS.gene062119:intron |
| TTTCATGCTGTTGCTCCTCC+TGG | 0.401020 | 2.3:-45778078 | MS.gene062119:CDS |
| GATTATGAACAAACTGAACT+TGG | 0.412329 | 2.3:-45778102 | MS.gene062119:CDS |
| AGATCGAGCTAAAGAACGTA+GGG | 0.526893 | 2.3:-45778139 | MS.gene062119:CDS |
| AAGTACAAGACGAACCTTCG+AGG | 0.547302 | 2.3:+45777908 | None:intergenic |
| TACCTGATATCAACGGTACC+AGG | 0.576314 | 2.3:+45778060 | None:intergenic |
| GAGATCGAGCTAAAGAACGT+AGG | 0.631640 | 2.3:-45778140 | MS.gene062119:CDS |
| GAGAAGAGCAAGTACCTCGA+AGG | 0.637606 | 2.3:-45777922 | MS.gene062119:CDS |
| GAAACTTTACCTGATATCAA+CGG | 0.665174 | 2.3:+45778053 | None:intergenic |
| CTGATATCAACGGTACCAGG+AGG | 0.732385 | 2.3:+45778063 | None:intergenic |
| GTACAGGGAAGAGAAACCAG+AGG | 0.759295 | 2.3:-45778184 | MS.gene062119:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATTGATGATGTTTTTTA+GGG | - | chr2.3:45778248-45778267 | MS.gene062119:intron | 15.0% |
| !!! | TAATATTGATGATGTTTTTT+AGG | - | chr2.3:45778247-45778266 | MS.gene062119:intron | 15.0% |
| !!! | TTTCAAAATCATTGTTTTTA+TGG | - | chr2.3:45777955-45777974 | MS.gene062119:intron | 15.0% |
| !!! | GTCAAAAAAATAGTTTCTTT+TGG | - | chr2.3:45778196-45778215 | MS.gene062119:intron | 20.0% |
| !!! | AAATCATTGTTTTTATGGAG+TGG | - | chr2.3:45777960-45777979 | MS.gene062119:intron | 25.0% |
| !!! | AATCATTGTTTTTATGGAGT+GGG | - | chr2.3:45777961-45777980 | MS.gene062119:intron | 25.0% |
| !!! | TCTCTATATTTTTGCAATTC+TGG | + | chr2.3:45778063-45778082 | None:intergenic | 25.0% |
| AAAACACCAACCACAAATAA+AGG | + | chr2.3:45778226-45778245 | None:intergenic | 30.0% | |
| AAACACCAACCACAAATAAA+GGG | + | chr2.3:45778225-45778244 | None:intergenic | 30.0% | |
| GAAACTTTACCTGATATCAA+CGG | + | chr2.3:45778169-45778188 | None:intergenic | 30.0% | |
| GATTATGAACAAACTGAACT+TGG | - | chr2.3:45778117-45778136 | MS.gene062119:CDS | 30.0% | |
| GATTTGATGAAACTTACTTG+CGG | + | chr2.3:45777926-45777945 | None:intergenic | 30.0% | |
| GGTCAATTAGTAACTTCTAT+GGG | - | chr2.3:45777986-45778005 | MS.gene062119:intron | 30.0% | |
| ! | AAAGTGTTTGGATTTGTACA+GGG | - | chr2.3:45778020-45778039 | MS.gene062119:intron | 30.0% |
| ! | AGTTCAGTTTGTTCATAATC+AGG | + | chr2.3:45778117-45778136 | None:intergenic | 30.0% |
| ! | GATGCAAATAGTAAAGTGTT+TGG | - | chr2.3:45778008-45778027 | MS.gene062119:intron | 30.0% |
| ! | TAAAGTGTTTGGATTTGTAC+AGG | - | chr2.3:45778019-45778038 | MS.gene062119:intron | 30.0% |
| !! | TTTTGGATACCCTTTATTTG+TGG | - | chr2.3:45778213-45778232 | MS.gene062119:intron | 30.0% |
| GGGTCAATTAGTAACTTCTA+TGG | - | chr2.3:45777985-45778004 | MS.gene062119:intron | 35.0% | |
| !!! | ATTGTTTTTATGGAGTGGGA+GGG | - | chr2.3:45777965-45777984 | MS.gene062119:intron | 35.0% |
| AGATCGAGCTAAAGAACGTA+GGG | - | chr2.3:45778080-45778099 | MS.gene062119:CDS | 40.0% | |
| GAAACTTACTTGCGGCAAAT+TGG | + | chr2.3:45777918-45777937 | None:intergenic | 40.0% | |
| ! | GGATACCCTTTATTTGTGGT+TGG | - | chr2.3:45778217-45778236 | MS.gene062119:intron | 40.0% |
| !!! | CATTGTTTTTATGGAGTGGG+AGG | - | chr2.3:45777964-45777983 | MS.gene062119:intron | 40.0% |
| !!! | TTTTGCAATTCTGGTTCCTC+TGG | + | chr2.3:45778054-45778073 | None:intergenic | 40.0% |
| AAGTACAAGACGAACCTTCG+AGG | + | chr2.3:45778314-45778333 | None:intergenic | 45.0% | |
| GAGATCGAGCTAAAGAACGT+AGG | - | chr2.3:45778079-45778098 | MS.gene062119:CDS | 45.0% | |
| TACCTGATATCAACGGTACC+AGG | + | chr2.3:45778162-45778181 | None:intergenic | 45.0% | |
| CTCCTGGTACCGTTGATATC+AGG | - | chr2.3:45778157-45778176 | MS.gene062119:CDS | 50.0% | |
| CTGATATCAACGGTACCAGG+AGG | + | chr2.3:45778159-45778178 | None:intergenic | 50.0% | |
| GAGAAGAGCAAGTACCTCGA+AGG | - | chr2.3:45778297-45778316 | MS.gene062119:intron | 50.0% | |
| GTACAGGGAAGAGAAACCAG+AGG | - | chr2.3:45778035-45778054 | MS.gene062119:intron | 50.0% | |
| TTTCATGCTGTTGCTCCTCC+TGG | - | chr2.3:45778141-45778160 | MS.gene062119:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 45777882 | 45778359 | 45777882 | ID=MS.gene062119 |
| chr2.3 | mRNA | 45777882 | 45778359 | 45777882 | ID=MS.gene062119.t1;Parent=MS.gene062119 |
| chr2.3 | exon | 45778313 | 45778359 | 45778313 | ID=MS.gene062119.t1.exon1;Parent=MS.gene062119.t1 |
| chr2.3 | CDS | 45778313 | 45778359 | 45778313 | ID=cds.MS.gene062119.t1;Parent=MS.gene062119.t1 |
| chr2.3 | exon | 45778063 | 45778200 | 45778063 | ID=MS.gene062119.t1.exon2;Parent=MS.gene062119.t1 |
| chr2.3 | CDS | 45778063 | 45778200 | 45778063 | ID=cds.MS.gene062119.t1;Parent=MS.gene062119.t1 |
| chr2.3 | exon | 45777882 | 45777972 | 45777882 | ID=MS.gene062119.t1.exon3;Parent=MS.gene062119.t1 |
| chr2.3 | CDS | 45777882 | 45777972 | 45777882 | ID=cds.MS.gene062119.t1;Parent=MS.gene062119.t1 |
| Gene Sequence |
| Protein sequence |
