AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062692

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062692.t1 MTR_5g062230 71.212 330 86 2 1 324 1 327 1.43e-171 489
MS.gene062692.t1 MTR_6g008560 70.997 331 89 1 1 324 1 331 1.48e-171 489
MS.gene062692.t1 MTR_6g039440 66.962 339 103 2 1 333 1 336 7.46e-165 472
MS.gene062692.t1 MTR_3g464190 65.407 344 109 3 1 337 1 341 1.61e-157 453
MS.gene062692.t1 MTR_4g048460 64.087 323 110 1 8 324 13 335 1.62e-154 442
MS.gene062692.t1 MTR_4g048460 64.087 323 110 1 8 324 13 335 2.22e-153 443
MS.gene062692.t1 MTR_5g073200 68.438 320 94 2 12 324 4 323 1.64e-150 435
MS.gene062692.t1 MTR_5g073260 66.250 320 100 2 13 324 7 326 5.16e-149 432
MS.gene062692.t1 MTR_5g094620 61.947 339 104 2 1 333 1 320 9.59e-149 430
MS.gene062692.t1 MTR_4g081460 58.333 324 126 2 9 324 14 336 7.49e-132 388
MS.gene062692.t1 MTR_2g082010 49.062 320 155 3 13 324 23 342 1.34e-105 321
MS.gene062692.t1 MTR_2g081980 47.590 332 162 5 15 337 12 340 1.11e-103 316
MS.gene062692.t1 MTR_8g007515 48.287 321 157 2 13 324 9 329 7.47e-102 311
MS.gene062692.t1 MTR_2g082060 45.697 337 169 7 1 324 3 338 3.50e-101 309
MS.gene062692.t1 MTR_4g019550 47.022 319 160 4 15 324 19 337 1.71e-96 297
MS.gene062692.t1 MTR_6g008560 75.429 175 43 0 150 324 1 175 2.46e-91 279
MS.gene062692.t1 MTR_4g045810 40.964 332 182 5 13 337 41 365 9.27e-76 244
MS.gene062692.t1 MTR_2g064425 42.492 313 171 4 17 324 53 361 4.03e-73 233
MS.gene062692.t1 MTR_2g064425 42.492 313 171 4 17 324 53 361 2.75e-71 233
MS.gene062692.t1 MTR_2g089120 42.697 267 140 2 61 324 84 340 1.86e-67 222
MS.gene062692.t1 MTR_8g057020 37.654 324 185 4 10 324 48 363 1.27e-65 218
MS.gene062692.t1 MTR_2g089130 41.573 267 143 2 61 324 84 340 6.68e-64 213
MS.gene062692.t1 MTR_2g064425 39.617 313 165 5 17 324 53 346 6.94e-63 206
MS.gene062692.t1 MTR_2g064425 39.617 313 165 5 17 324 53 346 2.60e-61 206
MS.gene062692.t1 MTR_2g082050 41.762 261 97 4 65 324 27 233 1.45e-60 201
MS.gene062692.t1 MTR_2g065450 35.423 319 198 4 10 324 51 365 2.22e-58 199
MS.gene062692.t1 MTR_5g094530 68.462 130 37 2 160 286 1 129 3.81e-56 182
MS.gene062692.t1 MTR_7g050990 64.122 131 38 1 194 324 28 149 2.30e-48 175
MS.gene062692.t1 MTR_4g092020 37.594 266 163 2 59 324 81 343 4.08e-48 171
MS.gene062692.t1 MTR_4g092010 32.573 307 196 6 25 324 34 336 8.65e-45 162
MS.gene062692.t1 MTR_2g010960 32.258 279 177 3 53 324 21 294 2.52e-44 160
MS.gene062692.t1 MTR_7g010710 30.120 332 220 5 1 324 1 328 2.71e-44 160
MS.gene062692.t1 MTR_3g465090 32.117 274 184 2 53 324 17 290 3.59e-37 140
MS.gene062692.t1 MTR_6g064980 32.103 271 180 4 57 324 21 290 2.39e-36 138
MS.gene062692.t1 MTR_3g063160 31.136 273 184 4 53 322 17 288 3.55e-36 133
MS.gene062692.t1 MTR_3g063170 30.909 275 186 4 53 324 17 290 1.41e-35 135
MS.gene062692.t1 MTR_3g052120 30.182 275 188 4 53 324 17 290 2.27e-33 129
MS.gene062692.t1 MTR_3g063140 30.545 275 187 4 53 324 17 290 3.27e-33 129
MS.gene062692.t1 MTR_3g063120 30.712 267 180 4 62 324 1 266 4.70e-33 128
MS.gene062692.t1 MTR_3g058160 29.091 275 171 7 64 317 269 540 4.04e-25 107
MS.gene062692.t1 MTR_3g058160 29.091 275 171 7 64 317 65 336 4.04e-25 106
MS.gene062692.t1 MTR_3g058160 29.091 275 171 7 64 317 256 527 4.31e-25 107
MS.gene062692.t1 MTR_2g064295 29.044 272 169 7 64 314 256 524 4.43e-25 107
MS.gene062692.t1 MTR_3g058160 29.091 275 171 7 64 317 276 547 4.53e-25 107
MS.gene062692.t1 MTR_7g011663 29.562 274 161 6 62 304 344 616 1.60e-23 102
MS.gene062692.t1 MTR_7g094970 27.148 291 179 7 62 322 206 493 2.05e-23 102
MS.gene062692.t1 MTR_3g058160 27.703 296 169 8 64 317 276 568 2.00e-22 99.4
MS.gene062692.t1 MTR_6g065040 31.383 188 126 3 139 324 2 188 1.71e-21 95.1
MS.gene062692.t1 MTR_5g030050 27.875 287 173 8 67 328 3 280 2.28e-21 93.6
MS.gene062692.t1 MTR_7g011770 28.179 291 180 7 62 324 240 529 5.12e-20 92.0
MS.gene062692.t1 MTR_2g012870 25.568 352 225 12 10 329 194 540 7.99e-20 91.3
MS.gene062692.t1 MTR_2g012900 24.451 364 228 15 10 329 194 554 3.15e-17 83.6
MS.gene062692.t1 MTR_5g081730 36.364 110 65 1 147 251 2 111 1.78e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062692.t1 AT5G23960 38.323 334 188 4 13 339 3 325 2.82e-80 255
MS.gene062692.t1 AT5G23960 38.994 318 186 3 13 323 3 319 1.48e-79 253
MS.gene062692.t1 AT1G70080 39.394 330 185 7 8 324 57 384 2.78e-72 236
MS.gene062692.t1 AT1G31950 38.629 321 169 5 22 324 70 380 5.62e-71 230
MS.gene062692.t1 AT1G31950 38.629 321 169 5 22 324 70 380 3.30e-70 230
MS.gene062692.t1 AT3G14490 37.725 334 189 6 17 338 60 386 3.36e-70 230
MS.gene062692.t1 AT3G14540 39.297 313 177 4 22 324 65 374 1.30e-69 226
MS.gene062692.t1 AT1G31950 38.019 313 181 4 22 324 70 379 3.25e-69 228
MS.gene062692.t1 AT3G14540 39.297 313 177 4 22 324 65 374 1.16e-68 226
MS.gene062692.t1 AT3G14520 38.535 314 178 5 22 324 68 377 8.14e-68 224
MS.gene062692.t1 AT5G44630 37.318 343 198 7 18 346 14 353 2.04e-64 214
MS.gene062692.t1 AT4G15870 38.658 313 175 7 25 324 75 383 1.76e-63 213
MS.gene062692.t1 AT4G20210 37.152 323 181 7 17 324 58 373 1.43e-61 207
MS.gene062692.t1 AT4G16730 35.000 320 178 5 53 348 33 346 4.71e-61 205
MS.gene062692.t1 AT3G29410 37.736 318 184 6 17 324 64 377 6.99e-61 206
MS.gene062692.t1 AT1G33750 37.540 309 181 5 24 324 73 377 7.21e-61 206
MS.gene062692.t1 AT4G20200 38.621 290 161 5 48 324 92 377 8.06e-60 203
MS.gene062692.t1 AT4G16730 35.621 306 167 5 67 348 1 300 6.60e-59 198
MS.gene062692.t1 AT3G29410 40.530 264 153 3 63 324 11 272 8.41e-59 198
MS.gene062692.t1 AT3G32030 33.934 333 201 6 2 324 54 377 1.02e-58 200
MS.gene062692.t1 AT4G16740 34.451 328 193 6 12 324 21 341 7.37e-58 192
MS.gene062692.t1 AT4G16740 34.451 328 193 6 12 324 21 341 7.37e-58 192
MS.gene062692.t1 AT4G16740 33.918 342 201 7 12 338 21 352 7.64e-58 192
MS.gene062692.t1 AT2G23230 35.799 338 190 10 16 340 55 378 2.69e-56 193
MS.gene062692.t1 AT2G23230 35.799 338 190 10 16 340 55 378 3.13e-56 193
MS.gene062692.t1 AT4G20230 35.000 320 186 7 25 329 70 382 3.50e-56 193
MS.gene062692.t1 AT4G20230 35.000 320 186 7 25 329 82 394 4.55e-56 193
MS.gene062692.t1 AT4G16740 34.000 350 205 8 4 338 14 352 6.20e-56 192
MS.gene062692.t1 AT3G29190 37.407 270 164 3 63 329 112 379 6.49e-56 192
MS.gene062692.t1 AT4G13300 35.714 322 191 7 25 334 20 337 1.57e-55 190
MS.gene062692.t1 AT1G66020 34.796 319 194 5 17 324 56 371 2.37e-55 187
MS.gene062692.t1 AT3G29110 36.677 319 188 7 17 324 55 370 7.14e-55 189
MS.gene062692.t1 AT1G66020 34.796 319 194 5 17 324 56 371 7.74e-54 187
MS.gene062692.t1 AT3G25820 31.965 341 210 8 15 341 45 377 1.06e-52 179
MS.gene062692.t1 AT3G25820 31.965 341 210 8 15 341 45 377 1.06e-52 179
MS.gene062692.t1 AT2G24210 34.211 342 201 9 10 337 39 370 1.77e-52 183
MS.gene062692.t1 AT3G25820 31.975 319 206 5 15 323 45 362 8.20e-52 178
MS.gene062692.t1 AT4G13280 36.129 310 182 7 27 324 22 327 9.89e-52 180
MS.gene062692.t1 AT4G13280 36.129 310 182 7 27 324 22 327 2.05e-51 179
MS.gene062692.t1 AT3G25830 31.975 319 206 5 15 323 45 362 1.44e-50 178
MS.gene062692.t1 AT3G25820 31.975 319 206 5 15 323 45 362 1.44e-50 178
MS.gene062692.t1 AT1G48800 33.642 324 196 8 17 327 63 380 6.58e-48 171
MS.gene062692.t1 AT3G25810 34.247 292 172 7 57 337 93 375 3.63e-47 169
MS.gene062692.t1 AT1G48820 34.494 316 172 8 17 324 63 351 1.56e-46 166
MS.gene062692.t1 AT1G61680 33.333 282 174 4 61 337 90 362 5.15e-43 157
MS.gene062692.t1 AT1G61680 32.624 282 169 5 61 337 90 355 1.12e-39 148
MS.gene062692.t1 AT5G48110 48.718 117 59 1 209 324 1 117 4.95e-30 117
MS.gene062692.t1 AT4G02780 28.788 264 152 6 62 294 329 587 1.59e-23 102
MS.gene062692.t1 AT1G79460 27.931 290 169 10 64 324 274 552 2.79e-19 89.7
MS.gene062692.t1 AT1G61120 26.056 284 184 5 62 324 283 561 2.03e-16 81.3

Find 62 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTCTTGCTGTGCTATTT+AGG 0.103456 6.4:+45408615 MS.gene062692:CDS
TCTACTTGCATTGGCAAAAT+TGG 0.119753 6.4:+45409534 MS.gene062692:CDS
TTCTCTTGCTGTGCTATTTA+GGG 0.189824 6.4:+45408616 MS.gene062692:CDS
ATGCAAGTAGAATTTCATTA+TGG 0.248221 6.4:-45409522 None:intergenic
AGCCTACATCAAAAGGAATT+TGG 0.257080 6.4:+45409574 MS.gene062692:CDS
TACAAGAGTTATGTTCAAAA+TGG 0.269625 6.4:+45408566 MS.gene062692:CDS
TCCTTATGCTTCAGAATCTA+TGG 0.272455 6.4:+45408227 MS.gene062692:CDS
AGACGAAATTAATCAAGTTT+TGG 0.301364 6.4:+45408535 MS.gene062692:CDS
CAGATTTCCACCCTAGCGTT+TGG 0.311610 6.4:+45408190 MS.gene062692:CDS
AGATTTCCACCCTAGCGTTT+GGG 0.325288 6.4:+45408191 MS.gene062692:CDS
TGCAAAACCGATTTCTCATT+AGG 0.335406 6.4:-45408156 None:intergenic
TGTGCGGCAATATTCATATC+AGG 0.335782 6.4:-45408413 None:intergenic
TTCCTTGCTCGTCCTTGAAT+TGG 0.345275 6.4:-45409246 None:intergenic
GAGTAGTAGAATGTTGCTTT+TGG 0.347068 6.4:+45409762 MS.gene062692:CDS
GATTGACGCAATATGTCGTT+TGG 0.362028 6.4:+45408493 MS.gene062692:CDS
AAACATATTGGAAGAGGCTT+TGG 0.373001 6.4:+45409348 MS.gene062692:CDS
CATCAATCCTAATGAGAAAT+CGG 0.381109 6.4:+45408149 MS.gene062692:CDS
TGCAAGTAGAATTTCATTAT+GGG 0.387262 6.4:-45409521 None:intergenic
GGATGAATCTTGCTCATAGA+TGG 0.390050 6.4:-45409500 None:intergenic
GGTTCATGGTGAAAACATAT+TGG 0.429119 6.4:+45409336 MS.gene062692:CDS
ATTGACGCAATATGTCGTTT+GGG 0.435447 6.4:+45408494 MS.gene062692:CDS
TTCTGGTCACCTTGAGTCCA+TGG 0.446304 6.4:+45409378 MS.gene062692:CDS
GCTCCAAAGCCTACATCAAA+AGG 0.450495 6.4:+45409567 MS.gene062692:CDS
TACGACTTCTTACATTGGAA+TGG 0.454510 6.4:+45410789 MS.gene062692:CDS
TAATGAAATTCTACTTGCAT+TGG 0.460272 6.4:+45409525 MS.gene062692:CDS
ACGACTTCTTACATTGGAAT+GGG 0.460983 6.4:+45410790 MS.gene062692:CDS
TTGCAAGAGAAGGACTCAAT+CGG 0.462800 6.4:-45409405 None:intergenic
ATCATCAATGTATGATAAAA+CGG 0.468585 6.4:-45409849 None:intergenic
GATGATACATATGATGCATA+TGG 0.472052 6.4:+45409866 MS.gene062692:CDS
TTCCTTGCTTGAGAATATTG+AGG 0.494460 6.4:-45409806 None:intergenic
CTGTGCTATTTAGGGTGTTG+AGG 0.502767 6.4:+45408624 MS.gene062692:CDS
GTATGAAGCCTCGCATATGA+TGG 0.509870 6.4:+45409315 MS.gene062692:CDS
TGGTGAAAACATATTGGAAG+AGG 0.510576 6.4:+45409342 MS.gene062692:CDS
ATACAGGTGGTGGAAGGAGT+TGG 0.512387 6.4:+45409706 MS.gene062692:intron
AGGACTCAATCGGGTGGCCA+TGG 0.512884 6.4:-45409395 None:intergenic
AGATGTGTTCAACCAATTCA+AGG 0.513117 6.4:+45409234 MS.gene062692:intron
ACCATAGATTCTGAAGCATA+AGG 0.518574 6.4:-45408228 None:intergenic
TAACTTCTTGCCACTAGCCT+AGG 0.521919 6.4:-45409472 None:intergenic
TAAGAAGTCGTAGCCAACAC+TGG 0.522936 6.4:-45410778 None:intergenic
GCCTCGCATATGATGGTTCA+TGG 0.524574 6.4:+45409322 MS.gene062692:CDS
AGCCTCAATATTCTCAAGCA+AGG 0.525283 6.4:+45409804 MS.gene062692:CDS
CATGGACTTTACGTTTCACC+AGG 0.534178 6.4:+45408650 MS.gene062692:CDS
AACATTGGTCGTAGATGTTG+AGG 0.541350 6.4:+45409279 MS.gene062692:CDS
TGCAAGAGAAGGACTCAATC+GGG 0.546913 6.4:-45409404 None:intergenic
TTTAGGGTGTTGAGGCAACA+TGG 0.552007 6.4:+45408632 MS.gene062692:CDS
ATCGGGTGGCCATGGACTCA+AGG 0.566605 6.4:-45409387 None:intergenic
CAGAAACTTGCCTAGGCTAG+TGG 0.568355 6.4:+45409462 MS.gene062692:CDS
ATCAAGTGGTTACCCAGTGT+TGG 0.570889 6.4:+45410765 MS.gene062692:CDS
ATAGCACAGCAAGAGAATGA+AGG 0.572946 6.4:-45408610 None:intergenic
CTTTATATACAGGTGGTGGA+AGG 0.574461 6.4:+45409700 MS.gene062692:intron
AGGAAACTTCAATGAAACAT+TGG 0.586045 6.4:+45409264 MS.gene062692:CDS
TTGGCTACGACTTCTTACAT+TGG 0.586299 6.4:+45410784 MS.gene062692:CDS
ACTACTCTATCTCGTGCATA+CGG 0.590301 6.4:-45409746 None:intergenic
CACATATCAACAATATCAAG+TGG 0.594145 6.4:+45410751 MS.gene062692:CDS
GATTTCCACCCTAGCGTTTG+GGG 0.596026 6.4:+45408192 MS.gene062692:CDS
AATCTCCCCAAACGCTAGGG+TGG 0.601357 6.4:-45408197 None:intergenic
AAGAAGTCGTAGCCAACACT+GGG 0.613680 6.4:-45410777 None:intergenic
AACCAATTCAAGGACGAGCA+AGG 0.615624 6.4:+45409244 MS.gene062692:CDS
ACATTGGTCGTAGATGTTGA+GGG 0.633793 6.4:+45409280 MS.gene062692:CDS
CTCTACGCAGAAACTTGCCT+AGG 0.636868 6.4:+45409455 MS.gene062692:CDS
ACCATGAACCATCATATGCG+AGG 0.638484 6.4:-45409323 None:intergenic
AAGAGAAGGACTCAATCGGG+TGG 0.695745 6.4:-45409401 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAAATTACATAAAAT+AGG + chr6.4:45408900-45408919 MS.gene062692:intron 10.0%
!! TAATTAAAAGAGAATAATAT+AGG - chr6.4:45408953-45408972 None:intergenic 10.0%
!! TGATTATATAGTAATATTAA+TGG + chr6.4:45409031-45409050 MS.gene062692:intron 10.0%
!!! AAAAAAAGTTCTTATTTAAT+AGG - chr6.4:45409071-45409090 None:intergenic 10.0%
!!! CATTTTTTATGTTTTTAATT+TGG + chr6.4:45408706-45408725 MS.gene062692:intron 10.0%
!! ATAAAGACAAAAAATAGTAT+AGG - chr6.4:45409686-45409705 None:intergenic 15.0%
!! TAATATGAATGTCTAAAAAA+TGG + chr6.4:45408831-45408850 MS.gene062692:intron 15.0%
!! TTAACATCATTTAATTACAT+AGG + chr6.4:45410592-45410611 MS.gene062692:intron 15.0%
!!! AATCAAAATTATTTTTAGGT+GGG + chr6.4:45410178-45410197 MS.gene062692:intron 15.0%
!!! ATTGTTGTTTAATATTATTG+TGG + chr6.4:45409193-45409212 MS.gene062692:intron 15.0%
!!! TATTTTTTGTCTTTATATAC+AGG + chr6.4:45409690-45409709 MS.gene062692:intron 15.0%
!!! TTAATTACATAGGTTTTTTT+TGG + chr6.4:45410602-45410621 MS.gene062692:intron 15.0%
!!! TTGCAATCAAAATTATTTTT+AGG + chr6.4:45410174-45410193 MS.gene062692:intron 15.0%
!! AATATTAATGGTATACATGT+AGG + chr6.4:45409043-45409062 MS.gene062692:intron 20.0%
!! ATCATCAATGTATGATAAAA+CGG - chr6.4:45409852-45409871 None:intergenic 20.0%
!! CATATGAAATAAATGTTAAC+AGG - chr6.4:45410635-45410654 None:intergenic 20.0%
!! CTGTTAACATTTATTTCATA+TGG + chr6.4:45410633-45410652 MS.gene062692:intron 20.0%
!! TCGTAGAATTAATATTACAA+AGG - chr6.4:45410509-45410528 None:intergenic 20.0%
!! TTCTTTAAACAATAGAGTAA+TGG + chr6.4:45410145-45410164 MS.gene062692:intron 20.0%
!!! AATTCTCTTTTTACTAAAAG+TGG - chr6.4:45408861-45408880 None:intergenic 20.0%
!!! ATTGTTTTATCTCTAAAGTT+TGG - chr6.4:45409975-45409994 None:intergenic 20.0%
!!! CAATCAAAATTATTTTTAGG+TGG + chr6.4:45410177-45410196 MS.gene062692:intron 20.0%
!!! TATCTTGACATTTTAATTAG+TGG + chr6.4:45410535-45410554 MS.gene062692:intron 20.0%
!!! TGAATTAGAACTTTTTACAA+AGG + chr6.4:45409898-45409917 MS.gene062692:CDS 20.0%
! AATTCTTTAAATCAACAGCA+AGG + chr6.4:45408255-45408274 MS.gene062692:intron 25.0%
! ATGCAAGTAGAATTTCATTA+TGG - chr6.4:45409525-45409544 None:intergenic 25.0%
! TAATGAAATTCTACTTGCAT+TGG + chr6.4:45409525-45409544 MS.gene062692:CDS 25.0%
! TACAAGAGTTATGTTCAAAA+TGG + chr6.4:45408566-45408585 MS.gene062692:CDS 25.0%
! TGCAAGTAGAATTTCATTAT+GGG - chr6.4:45409524-45409543 None:intergenic 25.0%
! TTCAAAAAACTTATTGTCCA+TGG + chr6.4:45410424-45410443 MS.gene062692:intron 25.0%
! TTGAGTAAAACAAATTAGAC+AGG + chr6.4:45408737-45408756 MS.gene062692:intron 25.0%
!! AGACGAAATTAATCAAGTTT+TGG + chr6.4:45408535-45408554 MS.gene062692:CDS 25.0%
!! GTAATATTTGACTTTGTCAT+GGG + chr6.4:45408802-45408821 MS.gene062692:intron 25.0%
!! TAAGTTGAGATATATATACC+TGG - chr6.4:45408671-45408690 None:intergenic 25.0%
!! TTTTTACAAAGGCAATTGAA+AGG + chr6.4:45409909-45409928 MS.gene062692:CDS 25.0%
!! TTTTTGTCTTTATATACAGG+TGG + chr6.4:45409693-45409712 MS.gene062692:intron 25.0%
!!! AACTCTTACTTCTTTTAAGA+AGG - chr6.4:45410324-45410343 None:intergenic 25.0%
!!! ACTCTTACTTCTTTTAAGAA+GGG - chr6.4:45410323-45410342 None:intergenic 25.0%
!!! TTTTAGTTGATCAATTTGTG+CGG - chr6.4:45408432-45408451 None:intergenic 25.0%
AAACTGAGTTCATATAACCA+TGG - chr6.4:45410444-45410463 None:intergenic 30.0%
AATTTATCCATCTTCGATCT+TGG + chr6.4:45410057-45410076 MS.gene062692:intron 30.0%
AGGAAACTTCAATGAAACAT+TGG + chr6.4:45409264-45409283 MS.gene062692:CDS 30.0%
ATGAAAAGTGATTGAAGAGT+AGG + chr6.4:45408289-45408308 MS.gene062692:intron 30.0%
CACATATCAACAATATCAAG+TGG + chr6.4:45410751-45410770 MS.gene062692:CDS 30.0%
CATCAATCCTAATGAGAAAT+CGG + chr6.4:45408149-45408168 MS.gene062692:CDS 30.0%
GATGATACATATGATGCATA+TGG + chr6.4:45409866-45409885 MS.gene062692:CDS 30.0%
TAAAGCAACTTCATGTAATC+CGG - chr6.4:45410227-45410246 None:intergenic 30.0%
TAACTATATACCAACAACAG+AGG + chr6.4:45410720-45410739 MS.gene062692:intron 30.0%
TGTTAAGATGTATCATCATG+AGG + chr6.4:45410345-45410364 MS.gene062692:intron 30.0%
! CGTAATATTTGACTTTGTCA+TGG + chr6.4:45408801-45408820 MS.gene062692:intron 30.0%
! GTTGCTTTATAAATCACTCT+TGG + chr6.4:45410238-45410257 MS.gene062692:intron 30.0%
! TTCCAAATTCCTTTTGATGT+AGG - chr6.4:45409579-45409598 None:intergenic 30.0%
! TTTTGCTAAATGCAGTTCAT+AGG + chr6.4:45408373-45408392 MS.gene062692:intron 30.0%
!! CTCTTACTTCTTTTAAGAAG+GGG - chr6.4:45410322-45410341 None:intergenic 30.0%
!! TTTACTTTTGTTGCAAGAGA+AGG - chr6.4:45409418-45409437 None:intergenic 30.0%
!!! AATCAAGTTGACTTTTTCCA+AGG - chr6.4:45408477-45408496 None:intergenic 30.0%
!!! AGTAGTAGAATGTTGCTTTT+GGG + chr6.4:45409763-45409782 MS.gene062692:CDS 30.0%
!!! ATCAAGTTGACTTTTTCCAA+GGG - chr6.4:45408476-45408495 None:intergenic 30.0%
AAACATATTGGAAGAGGCTT+TGG + chr6.4:45409348-45409367 MS.gene062692:CDS 35.0%
AATCCAAGCTTATATGAGTG+AGG + chr6.4:45410681-45410700 MS.gene062692:intron 35.0%
ACGACTTCTTACATTGGAAT+GGG + chr6.4:45410790-45410809 MS.gene062692:CDS 35.0%
AGATGTGTTCAACCAATTCA+AGG + chr6.4:45409234-45409253 MS.gene062692:intron 35.0%
AGCCTACATCAAAAGGAATT+TGG + chr6.4:45409574-45409593 MS.gene062692:CDS 35.0%
GAGCAAGAAATGAAGAAAGA+AGG + chr6.4:45410278-45410297 MS.gene062692:intron 35.0%
GGTTCATGGTGAAAACATAT+TGG + chr6.4:45409336-45409355 MS.gene062692:CDS 35.0%
TACGACTTCTTACATTGGAA+TGG + chr6.4:45410789-45410808 MS.gene062692:CDS 35.0%
TAGGTGGGATATTAATTGCT+TGG + chr6.4:45410193-45410212 MS.gene062692:intron 35.0%
TCCTTATGCTTCAGAATCTA+TGG + chr6.4:45408227-45408246 MS.gene062692:CDS 35.0%
TGGTGAAAACATATTGGAAG+AGG + chr6.4:45409342-45409361 MS.gene062692:CDS 35.0%
TTCATATCAGGTTCCTGTTA+TGG - chr6.4:45408404-45408423 None:intergenic 35.0%
TTCCTTGCTTGAGAATATTG+AGG - chr6.4:45409809-45409828 None:intergenic 35.0%
TTGTCTTTATATACAGGTGG+TGG + chr6.4:45409696-45409715 MS.gene062692:intron 35.0%
! ATTCCTTTTGATGTAGGCTT+TGG - chr6.4:45409573-45409592 None:intergenic 35.0%
! ATTGACGCAATATGTCGTTT+GGG + chr6.4:45408494-45408513 MS.gene062692:CDS 35.0%
! CTAGTCATCAAGTTTAGAGT+TGG + chr6.4:45410013-45410032 MS.gene062692:intron 35.0%
! TCTACTTGCATTGGCAAAAT+TGG + chr6.4:45409534-45409553 MS.gene062692:CDS 35.0%
! TGCAAAACCGATTTCTCATT+AGG - chr6.4:45408159-45408178 None:intergenic 35.0%
!! ACCATAGATTCTGAAGCATA+AGG - chr6.4:45408231-45408250 None:intergenic 35.0%
!! ATTCTCTTGCTGTGCTATTT+AGG + chr6.4:45408615-45408634 MS.gene062692:CDS 35.0%
!! TTCATAGGTTGATCCATAAC+AGG + chr6.4:45408388-45408407 MS.gene062692:intron 35.0%
!! TTCTCTTGCTGTGCTATTTA+GGG + chr6.4:45408616-45408635 MS.gene062692:CDS 35.0%
!! TTCTTTTAAGAAGGGGTCAA+GGG - chr6.4:45410315-45410334 None:intergenic 35.0%
!!! GAGTAGTAGAATGTTGCTTT+TGG + chr6.4:45409762-45409781 MS.gene062692:CDS 35.0%
AAAAAATCTCCCCAAACGCT+AGG - chr6.4:45408204-45408223 None:intergenic 40.0%
AAAAATCTCCCCAAACGCTA+GGG - chr6.4:45408203-45408222 None:intergenic 40.0%
AACATTGGTCGTAGATGTTG+AGG + chr6.4:45409279-45409298 MS.gene062692:CDS 40.0%
ACATTGGTCGTAGATGTTGA+GGG + chr6.4:45409280-45409299 MS.gene062692:CDS 40.0%
ACTACTCTATCTCGTGCATA+CGG - chr6.4:45409749-45409768 None:intergenic 40.0%
AGCCTCAATATTCTCAAGCA+AGG + chr6.4:45409804-45409823 MS.gene062692:CDS 40.0%
ATAGCACAGCAAGAGAATGA+AGG - chr6.4:45408613-45408632 None:intergenic 40.0%
ATAGTTAGCATTCAGCCATC+TGG - chr6.4:45410707-45410726 None:intergenic 40.0%
GATTGACGCAATATGTCGTT+TGG + chr6.4:45408493-45408512 MS.gene062692:CDS 40.0%
GGATGAATCTTGCTCATAGA+TGG - chr6.4:45409503-45409522 None:intergenic 40.0%
GTGGGATATTAATTGCTTGG+AGG + chr6.4:45410196-45410215 MS.gene062692:intron 40.0%
TAATTGCTTGGAGGATCTTC+CGG + chr6.4:45410205-45410224 MS.gene062692:intron 40.0%
TGTGCGGCAATATTCATATC+AGG - chr6.4:45408416-45408435 None:intergenic 40.0%
TTAGCAACCAAGATCGAAGA+TGG - chr6.4:45410067-45410086 None:intergenic 40.0%
TTGCAAGAGAAGGACTCAAT+CGG - chr6.4:45409408-45409427 None:intergenic 40.0%
TTGGCTACGACTTCTTACAT+TGG + chr6.4:45410784-45410803 MS.gene062692:CDS 40.0%
!! CTTCTTTTAAGAAGGGGTCA+AGG - chr6.4:45410316-45410335 None:intergenic 40.0%
!! CTTTATATACAGGTGGTGGA+AGG + chr6.4:45409700-45409719 MS.gene062692:intron 40.0%
AACCAATTCAAGGACGAGCA+AGG + chr6.4:45409244-45409263 MS.gene062692:CDS 45.0%
AAGAAGTCGTAGCCAACACT+GGG - chr6.4:45410780-45410799 None:intergenic 45.0%
ACCATGAACCATCATATGCG+AGG - chr6.4:45409326-45409345 None:intergenic 45.0%
AGATTTCCACCCTAGCGTTT+GGG + chr6.4:45408191-45408210 MS.gene062692:CDS 45.0%
ATCAAGTGGTTACCCAGTGT+TGG + chr6.4:45410765-45410784 MS.gene062692:CDS 45.0%
CATGGACTTTACGTTTCACC+AGG + chr6.4:45408650-45408669 MS.gene062692:CDS 45.0%
CTCAAAAACAGAGAAGCCCT+TGG + chr6.4:45408457-45408476 MS.gene062692:CDS 45.0%
CTTATATGAGTGAGGCCAGA+TGG + chr6.4:45410689-45410708 MS.gene062692:intron 45.0%
GCTCCAAAGCCTACATCAAA+AGG + chr6.4:45409567-45409586 MS.gene062692:CDS 45.0%
GTATGAAGCCTCGCATATGA+TGG + chr6.4:45409315-45409334 MS.gene062692:CDS 45.0%
TAAGAAGTCGTAGCCAACAC+TGG - chr6.4:45410781-45410800 None:intergenic 45.0%
TGCAAGAGAAGGACTCAATC+GGG - chr6.4:45409407-45409426 None:intergenic 45.0%
TGCATGTACTCCTCTGTTGT+TGG - chr6.4:45410733-45410752 None:intergenic 45.0%
TGGCCTCACTCATATAAGCT+TGG - chr6.4:45410687-45410706 None:intergenic 45.0%
TTCCTTGCTCGTCCTTGAAT+TGG - chr6.4:45409249-45409268 None:intergenic 45.0%
! TAACTTCTTGCCACTAGCCT+AGG - chr6.4:45409475-45409494 None:intergenic 45.0%
!! CTGTGCTATTTAGGGTGTTG+AGG + chr6.4:45408624-45408643 MS.gene062692:CDS 45.0%
!! TTTAGGGTGTTGAGGCAACA+TGG + chr6.4:45408632-45408651 MS.gene062692:CDS 45.0%
!!! GAGGCTTTGGCTTTTACTTC+TGG + chr6.4:45409361-45409380 MS.gene062692:CDS 45.0%
!!! ATTTTATAATAATAAAAGAT+AGG + chr6.4:45408878-45408897 MS.gene062692:intron 5.0%
AAGAGAAGGACTCAATCGGG+TGG - chr6.4:45409404-45409423 None:intergenic 50.0%
CAGAAACTTGCCTAGGCTAG+TGG + chr6.4:45409462-45409481 MS.gene062692:CDS 50.0%
CAGATTTCCACCCTAGCGTT+TGG + chr6.4:45408190-45408209 MS.gene062692:CDS 50.0%
CTCTACGCAGAAACTTGCCT+AGG + chr6.4:45409455-45409474 MS.gene062692:CDS 50.0%
GATTTCCACCCTAGCGTTTG+GGG + chr6.4:45408192-45408211 MS.gene062692:CDS 50.0%
GCCTCGCATATGATGGTTCA+TGG + chr6.4:45409322-45409341 MS.gene062692:CDS 50.0%
TTCTGGTCACCTTGAGTCCA+TGG + chr6.4:45409378-45409397 MS.gene062692:CDS 50.0%
!! ATACAGGTGGTGGAAGGAGT+TGG + chr6.4:45409706-45409725 MS.gene062692:intron 50.0%
AATCTCCCCAAACGCTAGGG+TGG - chr6.4:45408200-45408219 None:intergenic 55.0%
AGGACTCAATCGGGTGGCCA+TGG - chr6.4:45409398-45409417 None:intergenic 60.0%
ATCGGGTGGCCATGGACTCA+AGG - chr6.4:45409390-45409409 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 45408132 45410839 45408132 ID=MS.gene062692
chr6.4 mRNA 45408132 45410839 45408132 ID=MS.gene062692.t1;Parent=MS.gene062692
chr6.4 exon 45408132 45408248 45408132 ID=MS.gene062692.t1.exon1;Parent=MS.gene062692.t1
chr6.4 CDS 45408132 45408248 45408132 ID=cds.MS.gene062692.t1;Parent=MS.gene062692.t1
chr6.4 exon 45408410 45408671 45408410 ID=MS.gene062692.t1.exon2;Parent=MS.gene062692.t1
chr6.4 CDS 45408410 45408671 45408410 ID=cds.MS.gene062692.t1;Parent=MS.gene062692.t1
chr6.4 exon 45409236 45409608 45409236 ID=MS.gene062692.t1.exon3;Parent=MS.gene062692.t1
chr6.4 CDS 45409236 45409608 45409236 ID=cds.MS.gene062692.t1;Parent=MS.gene062692.t1
chr6.4 exon 45409712 45409930 45409712 ID=MS.gene062692.t1.exon4;Parent=MS.gene062692.t1
chr6.4 CDS 45409712 45409930 45409712 ID=cds.MS.gene062692.t1;Parent=MS.gene062692.t1
chr6.4 exon 45410740 45410839 45410740 ID=MS.gene062692.t1.exon5;Parent=MS.gene062692.t1
chr6.4 CDS 45410740 45410839 45410740 ID=cds.MS.gene062692.t1;Parent=MS.gene062692.t1
Gene Sequence

>MS.gene062692

ATGTCTTCAGCAGATTACATCAATCCTAATGAGAAATCGGTTTTGCAAAGAAATGTTGCAGATTTCCACCCTAGCGTTTGGGGAGATTTTTTCATTCCTTATGCTTCAGAATCTATGGTACGTAATTCTTTAAATCAACAGCAAGGTTATATATATTATGAAAAGTGATTGAAGAGTAGGTAGATTTACAACATAGATAGCACACACATATTTACTCATGAATGACAATAATGTAGACTCATTTTGCTAAATGCAGTTCATAGGTTGATCCATAACAGGAACCTGATATGAATATTGCCGCACAAATTGATCAACTAAAAATTGTCTCAAAAACAGAGAAGCCCTTGGAAAAAGTCAACTTGATTGACGCAATATGTCGTTTGGGCGTGAGCTATCATTTTGAAGACGAAATTAATCAAGTTTTGGAACATATTTACAAGAGTTATGTTCAAAATGGAGAAATAACTCTCAAAGACAACCTTCATTCTCTTGCTGTGCTATTTAGGGTGTTGAGGCAACATGGACTTTACGTTTCACCAGGTATATATATCTCAACTTATTCACATTAATGATTCATTTTTTATGTTTTTAATTTGGATCGCTAATTGAGTAAAACAAATTAGACAGGCAAACATTAAAAAAATCTAATCGTAAAATCAATATTAACTACGTAATATTTGACTTTGTCATGGGTGTGTCTAATATGAATGTCTAAAAAATGGTATACCACTTTTAGTAAAAAGAGAATTTTATAATAATAAAAGATAGGAAAAAAAATTACATAAAATAGGAGTGTCTTTCGTTTGATCAGATACACTCCTATATTATTCTCTTTTAATTATATTTTTAATTATTCTTTCTTAATTATATTTTTTTATATCATAGAAATAAACGCTTAATGATTATATAGTAATATTAATGGTATACATGTAGGATCCTATTAAATAAGAACTTTTTTTGTACTTCAGAAACACACTATACATTATCTAGATTGCATAATCAATTTTTCAATTTTGCTTTAGTTTCTTATTTATATATGTATATAAATTAATATGTCTCAAATTGTTGTTTAATATTATTGTGGTTGTCAAATAAAAATACTAGATGTGTTCAACCAATTCAAGGACGAGCAAGGAAACTTCAATGAAACATTGGTCGTAGATGTTGAGGGCATGTTAAACTTGTATGAAGCCTCGCATATGATGGTTCATGGTGAAAACATATTGGAAGAGGCTTTGGCTTTTACTTCTGGTCACCTTGAGTCCATGGCCACCCGATTGAGTCCTTCTCTTGCAACAAAAGTAAAACAAAGCTTAAGACAATCTCTACGCAGAAACTTGCCTAGGCTAGTGGCAAGAAGTTACATTTCCATCTATGAGCAAGATTCATCCCATAATGAAATTCTACTTGCATTGGCAAAATTGGATTTTAATATGCTCCAAAGCCTACATCAAAAGGAATTTGGAAACATTAGCAAGTAAGTACATCTTGAGCGTACATCAACATACAAAAGTATATTAAATTGAGTTCAAAAAATAATTATACTAATTTCCTATACTATTTTTTGTCTTTATATACAGGTGGTGGAAGGAGTTGGATATTGCTCAAAAACTACCGTATGCACGAGATAGAGTAGTAGAATGTTGCTTTTGGGTTATGACTGTATATTTTGAGCCTCAATATTCTCAAGCAAGGAAAATGATGTCAAAATTTATCTCCGTTTTATCATACATTGATGATACATATGATGCATATGGAACTACTGATGAATTAGAACTTTTTACAAAGGCAATTGAAAGGTTAGCACATCATACAATATTATTGTTAACTGAATTCGTGACCAAACTTTAGAGATAAAACAATATAAAGTTTATCTTTAATCTAGTCATCAAGTTTAGAGTTGGAATTTTTTTAATAATAACTTCAATTTATCCATCTTCGATCTTGGTTGCTAATTCAATTTTTTTCAAGAAATATAGACATTTTCTTCACATGTATTTGTACACGATATGATTCTTTAAACAATAGAGTAATGGTTAATATTGCAATCAAAATTATTTTTAGGTGGGATATTAATTGCTTGGAGGATCTTCCGGATTACATGAAGTTGCTTTATAAATCACTCTTGGATGTTTATGAAGAGATAGAGCAAGAAATGAAGAAAGAAGGAAGAGAATACACCCTTGACCCCTTCTTAAAAGAAGTAAGAGTTTTGTTAAGATGTATCATCATGAGGCTATTTGATTGAATGCAATGTTAAAAAAAATTCTACTATCAAAGAATAAAAATTAATTCAAAAAACTTATTGTCCATGGTTATATGAACTCAGTTTGTAGTAATAACGACAATAAATTTTGTTTGTCGTTAAAGAGCTCCTTTGTAATATTAATTCTACGAAAGATATATCTTGACATTTTAATTAGTGGTTAGATGAAAACTAAAAAAAATTGCTAATACAATTTAACATCATTTAATTACATAGGTTTTTTTTGGTACACAACCTGTTAACATTTATTTCATATGGTATACTAATGACAGTTCAAAAATATAATCCAAGCTTATATGAGTGAGGCCAGATGGCTGAATGCTAACTATATACCAACAACAGAGGAGTACATGCACATATCAACAATATCAAGTGGTTACCCAGTGTTGGCTACGACTTCTTACATTGGAATGGGAAACATAGCGACAAAAGACATCTTTGA
Protein sequence

>MS.gene062692.t1

MSSADYINPNEKSVLQRNVADFHPSVWGDFFIPYASESMEPDMNIAAQIDQLKIVSKTEKPLEKVNLIDAICRLGVSYHFEDEINQVLEHIYKSYVQNGEITLKDNLHSLAVLFRVLRQHGLYVSPDVFNQFKDEQGNFNETLVVDVEGMLNLYEASHMMVHGENILEEALAFTSGHLESMATRLSPSLATKVKQSLRQSLRRNLPRLVARSYISIYEQDSSHNEILLALAKLDFNMLQSLHQKEFGNISKWWKELDIAQKLPYARDRVVECCFWVMTVYFEPQYSQARKMMSKFISVLSYIDDTYDAYGTTDELELFTKAIERGVHAHINNIKWLPSVGYDFLHWNGKHSDKRHL