AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060118

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTCTTGCTGTGCTATTT+AGG 0.103456 6.2:-69603389 MS.gene060118:CDS
TCTACTTGCATTGGCAAAAT+TGG 0.119753 6.2:-69602427 MS.gene060118:CDS
TTCTCTTGCTGTGCTATTTA+GGG 0.189824 6.2:-69603388 MS.gene060118:CDS
TTTCCTTGCTTGAGAATATT+GGG 0.241792 6.2:+69602154 None:intergenic
ATGCAAGTAGAATTTCATTA+TGG 0.248221 6.2:+69602439 None:intergenic
AGCCTACATCAAAAGGAATT+TGG 0.257080 6.2:-69602387 MS.gene060118:CDS
TACAAGAGTTATGTTCAAAA+TGG 0.269625 6.2:-69603438 MS.gene060118:CDS
TCCTTATGCTTCAGAATCTA+TGG 0.272455 6.2:-69603904 MS.gene060118:intron
AGACGAAATTAATCAAGTTT+TGG 0.301364 6.2:-69603469 MS.gene060118:CDS
GTCATCAATGTATGATAAAA+TGG 0.310334 6.2:+69602112 None:intergenic
CAGATTTCCACCCTAGCGTT+TGG 0.311610 6.2:-69603941 MS.gene060118:CDS
AGATTTCCACCCTAGCGTTT+GGG 0.325288 6.2:-69603940 MS.gene060118:CDS
TGCAAAACCGATTTCTCATT+AGG 0.335406 6.2:+69603975 None:intergenic
TAATTGCTTGGAGGATCTTC+CGG 0.335446 6.2:-69601750 MS.gene060118:CDS
TTCCTTGCTCGTCCTTGAAT+TGG 0.345275 6.2:+69602715 None:intergenic
GAGTAGTAGAATGTTGCTTT+TGG 0.347068 6.2:-69602199 MS.gene060118:CDS
TTTGCCGCAGTATTCATATC+AGG 0.352130 6.2:+69603609 None:intergenic
CATCAATCCTAATGAGAAAT+CGG 0.381109 6.2:-69603982 MS.gene060118:CDS
TGCAAGTAGAATTTCATTAT+GGG 0.387262 6.2:+69602440 None:intergenic
GGATGAATCTTGCTCATAGA+TGG 0.390050 6.2:+69602461 None:intergenic
GATTGACTCAATATGTCGTT+TGG 0.390339 6.2:-69603511 MS.gene060118:CDS
TAAAGCAACTTCGTGTAATC+CGG 0.404734 6.2:+69601731 None:intergenic
GTTGCTTTATAAATCACTCT+TGG 0.404999 6.2:-69601717 MS.gene060118:CDS
TTCTGGTCACCTTGAGTCCA+TGG 0.446304 6.2:-69602583 MS.gene060118:CDS
GCTCCAAAGCCTACATCAAA+AGG 0.450495 6.2:-69602394 MS.gene060118:CDS
TAGGTGGGATATTAATTGCT+TGG 0.452782 6.2:-69601762 MS.gene060118:CDS
TAATGAAATTCTACTTGCAT+TGG 0.460272 6.2:-69602436 MS.gene060118:CDS
GATGACACATATGATGCATA+TGG 0.467097 6.2:-69602095 MS.gene060118:CDS
GAACAAGAAATGAAGAAAGA+AGG 0.470516 6.2:-69601677 MS.gene060118:CDS
ATTGACTCAATATGTCGTTT+GGG 0.473862 6.2:-69603510 MS.gene060118:CDS
TTGCAAGAGAAGGACTCAAT+CGG 0.484754 6.2:+69602556 None:intergenic
ATATTGGGGCTCAAAATATA+CGG 0.495144 6.2:+69602169 None:intergenic
CTGTGCTATTTAGGGTGTTG+AGG 0.502767 6.2:-69603380 MS.gene060118:CDS
CATGGACTTTACGTTTCACC+TGG 0.512238 6.2:-69603354 MS.gene060118:intron
AGGACTCAATCGGGTGGCCA+TGG 0.512884 6.2:+69602566 None:intergenic
AGATGTGTTCAACCAATTCA+AGG 0.513117 6.2:-69602727 MS.gene060118:CDS
ACCATAGATTCTGAAGCATA+AGG 0.518574 6.2:+69603903 None:intergenic
TAACTTCTTGCCACTAGCCT+AGG 0.521919 6.2:+69602489 None:intergenic
GCCTCACATATGATGGTTCA+TGG 0.522278 6.2:-69602639 MS.gene060118:CDS
ATATAGGTGGTGGAAGGAGT+TGG 0.524102 6.2:-69602255 MS.gene060118:intron
GTGGGATATTAATTGCTTGG+AGG 0.529634 6.2:-69601759 MS.gene060118:CDS
AACATTGGTCAGAGATGTTG+AGG 0.533818 6.2:-69602682 MS.gene060118:CDS
TTCCTTGCTTGAGAATATTG+GGG 0.534381 6.2:+69602155 None:intergenic
TGCAAGAGAAGGACTCAATC+GGG 0.546913 6.2:+69602557 None:intergenic
AGCCCCAATATTCTCAAGCA+AGG 0.547455 6.2:-69602157 MS.gene060118:CDS
TTTAGGGTGTTGAGGCAACA+TGG 0.552007 6.2:-69603372 MS.gene060118:CDS
TTTACTTGTGTTGCAAGAGA+AGG 0.558925 6.2:+69602546 None:intergenic
ATCGGGTGGCCATGGACTCA+AGG 0.566605 6.2:+69602574 None:intergenic
GTATGAAGCCTCACATATGA+TGG 0.567934 6.2:-69602646 MS.gene060118:CDS
ATAGCACAGCAAGAGAATGA+AGG 0.572946 6.2:+69603394 None:intergenic
AGGAAACTTCAGTGAAACAT+TGG 0.576091 6.2:-69602697 MS.gene060118:CDS
CTTTATATATAGGTGGTGGA+AGG 0.588928 6.2:-69602261 MS.gene060118:intron
ACTACTCTATCTCGTGCATA+CGG 0.590301 6.2:+69602215 None:intergenic
GATTTCCACCCTAGCGTTTG+GGG 0.596026 6.2:-69603939 MS.gene060118:CDS
AATCTCCCCAAACGCTAGGG+TGG 0.601357 6.2:+69603934 None:intergenic
AACCAATTCAAGGACGAGCA+AGG 0.615624 6.2:-69602717 MS.gene060118:CDS
ACCATGAACCATCATATGTG+AGG 0.634158 6.2:+69602638 None:intergenic
ACATTGGTCAGAGATGTTGA+GGG 0.666287 6.2:-69602681 MS.gene060118:CDS
AAGAGAAGGACTCAATCGGG+TGG 0.695745 6.2:+69602560 None:intergenic
GGAACCTGATATGAATACTG+CGG 0.696636 6.2:-69603613 MS.gene060118:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTATAAACTAATATTAA+TGG - chr6.2:69602675-69602694 MS.gene060118:CDS 10.0%
!!! CTTTTTTTATGTTTTTAATT+TGG - chr6.2:69602305-69602324 MS.gene060118:intron 10.0%
!!! TATTTTTTGTCTTTATATAT+AGG - chr6.2:69603334-69603353 MS.gene060118:intron 10.0%
!! AATTGATAAACTAAAAAAGA+AGG - chr6.2:69602019-69602038 MS.gene060118:intron 15.0%
!! ATAAAGACAAAAAATAGTAT+AGG + chr6.2:69603330-69603349 None:intergenic 15.0%
!! ATGAGTAAATAAATAGTATA+AGG + chr6.2:69601854-69601873 None:intergenic 15.0%
!! TAATATGAATGTCTAAAAAA+TGG - chr6.2:69602437-69602456 MS.gene060118:CDS 15.0%
!!! ATACTTTTATATTTTGATGT+AGG + chr6.2:69603272-69603291 None:intergenic 15.0%
!!! ATTGTTGTTTAATATTATTG+TGG - chr6.2:69602837-69602856 MS.gene060118:intron 15.0%
!! AAGACACTTAATTATTGTTA+TGG + chr6.2:69601927-69601946 None:intergenic 20.0%
!! AATATTAATGGTATACATGT+AGG - chr6.2:69602687-69602706 MS.gene060118:CDS 20.0%
!! GAGAATAATTAAAAGAAATG+AGG + chr6.2:69602613-69602632 None:intergenic 20.0%
!!! AATCAAAATGATTTTTAGGT+GGG - chr6.2:69603828-69603847 MS.gene060118:intron 20.0%
!!! ATTGTTTTATCTCTAAAGTT+TGG + chr6.2:69603625-69603644 None:intergenic 20.0%
!!! CTTTTTACTAAAAGATTTAC+AGG + chr6.2:69602477-69602496 None:intergenic 20.0%
!!! TATATGTTTTGAATACATAC+AGG - chr6.2:69601971-69601990 MS.gene060118:intron 20.0%
!!! TTGCAATCAAAATGATTTTT+AGG - chr6.2:69603824-69603843 MS.gene060118:intron 20.0%
!!! TTTTTGTCTTTATATATAGG+TGG - chr6.2:69603337-69603356 MS.gene060118:intron 20.0%
! ATGCAAGTAGAATTTCATTA+TGG + chr6.2:69603169-69603188 None:intergenic 25.0%
! GTCATCAATGTATGATAAAA+TGG + chr6.2:69603496-69603515 None:intergenic 25.0%
! TAATGAAATTCTACTTGCAT+TGG - chr6.2:69603169-69603188 MS.gene060118:intron 25.0%
! TACAAGAGTTATGTTCAAAA+TGG - chr6.2:69602167-69602186 MS.gene060118:CDS 25.0%
! TGCAAGTAGAATTTCATTAT+GGG + chr6.2:69603168-69603187 None:intergenic 25.0%
! TTGAGTAAAACAAATTAGAG+TGG - chr6.2:69602336-69602355 MS.gene060118:intron 25.0%
!! AATAAGTTGAGATATATACC+AGG + chr6.2:69602272-69602291 None:intergenic 25.0%
!! AGACGAAATTAATCAAGTTT+TGG - chr6.2:69602136-69602155 MS.gene060118:CDS 25.0%
!! GTAATATTTGACTTTGTCAT+GGG - chr6.2:69602408-69602427 MS.gene060118:CDS 25.0%
!! TGAATTAGAACTTTTTACTC+AGG - chr6.2:69603542-69603561 MS.gene060118:CDS 25.0%
!! TTCTTTTAACAATAGAGCAA+TGG - chr6.2:69603795-69603814 MS.gene060118:intron 25.0%
!!! AACTCTTACTTCTTTTAAGA+AGG + chr6.2:69603974-69603993 None:intergenic 25.0%
!!! ACTCTTACTTCTTTTAAGAA+GGG + chr6.2:69603973-69603992 None:intergenic 25.0%
!!! CAATCAAAATGATTTTTAGG+TGG - chr6.2:69603827-69603846 MS.gene060118:intron 25.0%
AAATGTGAGTAACTTGAATC+TGG + chr6.2:69601830-69601849 None:intergenic 30.0%
AATGTGAGTAACTTGAATCT+GGG + chr6.2:69601829-69601848 None:intergenic 30.0%
AATTTACCCATCTTCAATCT+TGG - chr6.2:69603707-69603726 MS.gene060118:intron 30.0%
ATATTGGGGCTCAAAATATA+CGG + chr6.2:69603439-69603458 None:intergenic 30.0%
ATGTGAACATTACACAGATA+AGG + chr6.2:69602590-69602609 None:intergenic 30.0%
CATCAATCCTAATGAGAAAT+CGG - chr6.2:69601623-69601642 MS.gene060118:CDS 30.0%
GAACAAGAAATGAAGAAAGA+AGG - chr6.2:69603928-69603947 MS.gene060118:CDS 30.0%
GGTTCATGGTGAAAAAATAT+TGG - chr6.2:69602980-69602999 MS.gene060118:intron 30.0%
TGGTGAAAAAATATTGGAAG+AGG - chr6.2:69602986-69603005 MS.gene060118:intron 30.0%
TTGTCTTTATATATAGGTGG+TGG - chr6.2:69603340-69603359 MS.gene060118:intron 30.0%
TTTCCTTGCTTGAGAATATT+GGG + chr6.2:69603454-69603473 None:intergenic 30.0%
! ATTGACTCAATATGTCGTTT+GGG - chr6.2:69602095-69602114 MS.gene060118:CDS 30.0%
! CGTAATATTTGACTTTGTCA+TGG - chr6.2:69602407-69602426 MS.gene060118:CDS 30.0%
! GTTGCTTTATAAATCACTCT+TGG - chr6.2:69603888-69603907 MS.gene060118:intron 30.0%
! TTCCAAATTCCTTTTGATGT+AGG + chr6.2:69603223-69603242 None:intergenic 30.0%
! TTTTCCTTGCTTGAGAATAT+TGG + chr6.2:69603455-69603474 None:intergenic 30.0%
! TTTTGCTAAATGCAGTTCAT+AGG - chr6.2:69601896-69601915 MS.gene060118:intron 30.0%
! TTTTTACTCAGGCAATTGAA+AGG - chr6.2:69603553-69603572 MS.gene060118:CDS 30.0%
!! AATAGTGATTGAAGAGTACT+AGG - chr6.2:69601766-69601785 MS.gene060118:intron 30.0%
!! CTCTTACTTCTTTTAAGAAG+GGG + chr6.2:69603972-69603991 None:intergenic 30.0%
!! TGTTTCTGAAGTACGAAAAA+GGG + chr6.2:69602729-69602748 None:intergenic 30.0%
!!! AATCAAGTTGACTTTTTCCA+AGG + chr6.2:69602078-69602097 None:intergenic 30.0%
!!! AGTAGTAGAATGTTGCTTTT+GGG - chr6.2:69603407-69603426 MS.gene060118:CDS 30.0%
!!! ATCAAGTTGACTTTTTCCAA+GGG + chr6.2:69602077-69602096 None:intergenic 30.0%
AAAGATTTACAGGCTAACAG+TGG + chr6.2:69602467-69602486 None:intergenic 35.0%
AGATGTGTTCAACCAATTCA+AGG - chr6.2:69602878-69602897 MS.gene060118:intron 35.0%
AGCCTACATCAAAAGGAATT+TGG - chr6.2:69603218-69603237 MS.gene060118:intron 35.0%
AGGAAACTTCAGTGAAACAT+TGG - chr6.2:69602908-69602927 MS.gene060118:intron 35.0%
GAATCTGGGCTAATATTAAG+TGG + chr6.2:69601815-69601834 None:intergenic 35.0%
GATGACACATATGATGCATA+TGG - chr6.2:69603510-69603529 MS.gene060118:CDS 35.0%
TAAAGCAACTTCGTGTAATC+CGG + chr6.2:69603877-69603896 None:intergenic 35.0%
TAGCAACCAAGATTGAAGAT+GGG + chr6.2:69603716-69603735 None:intergenic 35.0%
TAGGTGGGATATTAATTGCT+TGG - chr6.2:69603843-69603862 MS.gene060118:intron 35.0%
TCCTTATGCTTCAGAATCTA+TGG - chr6.2:69601701-69601720 MS.gene060118:CDS 35.0%
TTAGCAACCAAGATTGAAGA+TGG + chr6.2:69603717-69603736 None:intergenic 35.0%
TTCCTTGCTTGAGAATATTG+GGG + chr6.2:69603453-69603472 None:intergenic 35.0%
TTTACTTGTGTTGCAAGAGA+AGG + chr6.2:69603062-69603081 None:intergenic 35.0%
! AAAAATATTGGAAGAGGCGT+TGG - chr6.2:69602992-69603011 MS.gene060118:intron 35.0%
! ATTCCTTTTGATGTAGGCTT+TGG + chr6.2:69603217-69603236 None:intergenic 35.0%
! CTAGTCATCAAGTTTAGAGT+TGG - chr6.2:69603663-69603682 MS.gene060118:intron 35.0%
! GATTGACTCAATATGTCGTT+TGG - chr6.2:69602094-69602113 MS.gene060118:CDS 35.0%
! TCTACTTGCATTGGCAAAAT+TGG - chr6.2:69603178-69603197 MS.gene060118:intron 35.0%
! TGCAAAACCGATTTCTCATT+AGG + chr6.2:69601633-69601652 None:intergenic 35.0%
!! ACCATAGATTCTGAAGCATA+AGG + chr6.2:69601705-69601724 None:intergenic 35.0%
!! ATTCTCTTGCTGTGCTATTT+AGG - chr6.2:69602216-69602235 MS.gene060118:CDS 35.0%
!! CTTTATATATAGGTGGTGGA+AGG - chr6.2:69603344-69603363 MS.gene060118:intron 35.0%
!! GTGTTTCTGAAGTACGAAAA+AGG + chr6.2:69602730-69602749 None:intergenic 35.0%
!! TTCTCTTGCTGTGCTATTTA+GGG - chr6.2:69602217-69602236 MS.gene060118:CDS 35.0%
!! TTCTTTTAAGAAGGGGTCAA+GGG + chr6.2:69603965-69603984 None:intergenic 35.0%
!!! GAGTAGTAGAATGTTGCTTT+TGG - chr6.2:69603406-69603425 MS.gene060118:CDS 35.0%
AAAAAATCTCCCCAAACGCT+AGG + chr6.2:69601678-69601697 None:intergenic 40.0%
AAAAATCTCCCCAAACGCTA+GGG + chr6.2:69601677-69601696 None:intergenic 40.0%
AACATTGGTCAGAGATGTTG+AGG - chr6.2:69602923-69602942 MS.gene060118:intron 40.0%
ACATTGGTCAGAGATGTTGA+GGG - chr6.2:69602924-69602943 MS.gene060118:intron 40.0%
ACCATGAACCATCATATGTG+AGG + chr6.2:69602970-69602989 None:intergenic 40.0%
ACTACTCTATCTCGTGCATA+CGG + chr6.2:69603393-69603412 None:intergenic 40.0%
ATAGCACAGCAAGAGAATGA+AGG + chr6.2:69602214-69602233 None:intergenic 40.0%
CTCAAAAACAGAGAAACCCT+TGG - chr6.2:69602058-69602077 MS.gene060118:CDS 40.0%
GGAACCTGATATGAATACTG+CGG - chr6.2:69601992-69602011 MS.gene060118:intron 40.0%
GGATGAATCTTGCTCATAGA+TGG + chr6.2:69603147-69603166 None:intergenic 40.0%
GTATGAAGCCTCACATATGA+TGG - chr6.2:69602959-69602978 MS.gene060118:intron 40.0%
GTGGGATATTAATTGCTTGG+AGG - chr6.2:69603846-69603865 MS.gene060118:intron 40.0%
TAATTGCTTGGAGGATCTTC+CGG - chr6.2:69603855-69603874 MS.gene060118:intron 40.0%
TTGCAAGAGAAGGACTCAAT+CGG + chr6.2:69603052-69603071 None:intergenic 40.0%
TTTGCCGCAGTATTCATATC+AGG + chr6.2:69601999-69602018 None:intergenic 40.0%
!! AGGAGTGTCTTTCGTTTGAT+CGG - chr6.2:69602547-69602566 MS.gene060118:CDS 40.0%
!! CTTCTTTTAAGAAGGGGTCA+AGG + chr6.2:69603966-69603985 None:intergenic 40.0%
AACCAATTCAAGGACGAGCA+AGG - chr6.2:69602888-69602907 MS.gene060118:intron 45.0%
AGATTTCCACCCTAGCGTTT+GGG - chr6.2:69601665-69601684 MS.gene060118:CDS 45.0%
AGCCCCAATATTCTCAAGCA+AGG - chr6.2:69603448-69603467 MS.gene060118:CDS 45.0%
CAAAAACTTGCCTAGGCTAG+TGG - chr6.2:69603106-69603125 MS.gene060118:intron 45.0%
CATGGACTTTACGTTTCACC+TGG - chr6.2:69602251-69602270 MS.gene060118:CDS 45.0%
GCCTCACATATGATGGTTCA+TGG - chr6.2:69602966-69602985 MS.gene060118:intron 45.0%
GCTCCAAAGCCTACATCAAA+AGG - chr6.2:69603211-69603230 MS.gene060118:intron 45.0%
TGCAAGAGAAGGACTCAATC+GGG + chr6.2:69603051-69603070 None:intergenic 45.0%
TTCCTTGCTCGTCCTTGAAT+TGG + chr6.2:69602893-69602912 None:intergenic 45.0%
! TAACTTCTTGCCACTAGCCT+AGG + chr6.2:69603119-69603138 None:intergenic 45.0%
!! ATATAGGTGGTGGAAGGAGT+TGG - chr6.2:69603350-69603369 MS.gene060118:intron 45.0%
!! CTGTGCTATTTAGGGTGTTG+AGG - chr6.2:69602225-69602244 MS.gene060118:CDS 45.0%
!! TTTAGGGTGTTGAGGCAACA+TGG - chr6.2:69602233-69602252 MS.gene060118:CDS 45.0%
!!! AATTTTTTTTTACATAAAAT+AGG - chr6.2:69602527-69602546 MS.gene060118:CDS 5.0%
AAGAGAAGGACTCAATCGGG+TGG + chr6.2:69603048-69603067 None:intergenic 50.0%
CAGATTTCCACCCTAGCGTT+TGG - chr6.2:69601664-69601683 MS.gene060118:CDS 50.0%
CTCTGCGCAAAAACTTGCCT+AGG - chr6.2:69603099-69603118 MS.gene060118:intron 50.0%
GATTTCCACCCTAGCGTTTG+GGG - chr6.2:69601666-69601685 MS.gene060118:CDS 50.0%
TTCTGGTCACCTTGAGTCCA+TGG - chr6.2:69603022-69603041 MS.gene060118:intron 50.0%
!!! GAGGCGTTGGCTTTTACTTC+TGG - chr6.2:69603005-69603024 MS.gene060118:intron 50.0%
AATCTCCCCAAACGCTAGGG+TGG + chr6.2:69601674-69601693 None:intergenic 55.0%
AGGACTCAATCGGGTGGCCA+TGG + chr6.2:69603042-69603061 None:intergenic 60.0%
ATCGGGTGGCCATGGACTCA+AGG + chr6.2:69603034-69603053 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 69601619 69604008 69601619 ID=MS.gene060118
chr6.2 mRNA 69601619 69604008 69601619 ID=MS.gene060118.t1;Parent=MS.gene060118
chr6.2 exon 69603905 69604008 69603905 ID=MS.gene060118.t1.exon1;Parent=MS.gene060118.t1
chr6.2 CDS 69603905 69604008 69603905 ID=cds.MS.gene060118.t1;Parent=MS.gene060118.t1
chr6.2 exon 69603355 69603634 69603355 ID=MS.gene060118.t1.exon2;Parent=MS.gene060118.t1
chr6.2 CDS 69603355 69603634 69603355 ID=cds.MS.gene060118.t1;Parent=MS.gene060118.t1
chr6.2 exon 69602375 69602747 69602375 ID=MS.gene060118.t1.exon3;Parent=MS.gene060118.t1
chr6.2 CDS 69602375 69602747 69602375 ID=cds.MS.gene060118.t1;Parent=MS.gene060118.t1
chr6.2 exon 69602053 69602271 69602053 ID=MS.gene060118.t1.exon4;Parent=MS.gene060118.t1
chr6.2 CDS 69602053 69602271 69602053 ID=cds.MS.gene060118.t1;Parent=MS.gene060118.t1
chr6.2 exon 69601619 69601781 69601619 ID=MS.gene060118.t1.exon5;Parent=MS.gene060118.t1
chr6.2 CDS 69601619 69601781 69601619 ID=cds.MS.gene060118.t1;Parent=MS.gene060118.t1
Gene Sequence

>MS.gene060118

ATTACATCAATCCTAATGAGAAATCGGTTTTGCAAAGAAATGTTGCAGATTTCCACCCTAGCGTTTGGGGAGATTTTTTCATTCCTTATGCTTCAGAATCTATGGTACGTAATTCTTTAAATCAACATCAAGTTTATATATATTATGAATAGTGATTGAAGAGTACTAGGAACATTATCACGTATGCAATAAACCACTTAATATTAGCCCAGATTCAAGTTACTCACATTTACCTTATACTATTTATTTACTCATGAATGACAATAATGTAGACTCATTTTGCTAAATGCAGTTCATAGGTTGATCCATAACAATAATTAAGTGTCTTTTATCAATTTATTCTAAAATCATATATATGTTTTGAATACATACAGGAACCTGATATGAATACTGCGGCAAAAATTGATAAACTAAAAAAGAAGGTTAGACAAATGCTTGTCTCAAAAACAGAGAAACCCTTGGAAAAAGTCAACTTGATTGACTCAATATGTCGTTTGGGTGTGAGCTATCATTTTGAAGACGAAATTAATCAAGTTTTGGAACATATTTACAAGAGTTATGTTCAAAATGGAGAAATAACTCTCAAAGACAACCTTCATTCTCTTGCTGTGCTATTTAGGGTGTTGAGGCAACATGGACTTTACGTTTCACCTGGTATATATCTCAACTTATTCACATTAATGATTCTTTTTTTATGTTTTTAATTTGGATCGCAAATTGAGTAAAACAAATTAGAGTGGTAAACATTAAAAAAAATCTAATTGTAAAATCAATACTTAAATTAACAACGTAATATTTGACTTTGTCATGGGTGTGTCTAATATGAATGTCTAAAAAATGGCATACCACTGTTAGCCTGTAAATCTTTTAGTAAAAAGAGAATTTTATAATAATAAAAGATAGAAAATAATTTTTTTTTACATAAAATAGGAGTGTCTTTCGTTTGATCGGATACATATTAGACAACTCCTTATCTGTGTAATGTTCACATCCTCATTTCTTTTAATTATTCTCTTTTAATTATTTTTTTTTTATATCATAGAAATAGACACTTAATGATTATAAACTAATATTAATGGTATACATGTAGGATCTTATTAAGTAAGACCCTTTTTCGTACTTCAGAAACACGCTATACATTATCTAGATTGCATAATGAATTTTTCAATCTTGCTTTAGCTTCTTATTTATATATATTTATAAATTAATATGTCTCAAATTGTTGTTTAATATTATTGTGGTTGTCTAATAAAAATACTAGATGTGTTCAACCAATTCAAGGACGAGCAAGGAAACTTCAGTGAAACATTGGTCAGAGATGTTGAGGGCATGTTAAACTTGTATGAAGCCTCACATATGATGGTTCATGGTGAAAAAATATTGGAAGAGGCGTTGGCTTTTACTTCTGGTCACCTTGAGTCCATGGCCACCCGATTGAGTCCTTCTCTTGCAACACAAGTAAAACAAAGCTTAAGACAATCTCTGCGCAAAAACTTGCCTAGGCTAGTGGCAAGAAGTTACATTTCCATCTATGAGCAAGATTCATCCCATAATGAAATTCTACTTGCATTGGCAAAATTGGATTTTAATATGCTCCAAAGCCTACATCAAAAGGAATTTGGAAACATTAGCAAGTAAGTACATTTTGAGCCTACATCAAAATATAAAAGTATATTAAATTGAGTTCAAAAAATAATGATACTAATTTCCTATACTATTTTTTGTCTTTATATATAGGTGGTGGAAGGAGTTGGATATTGCTCAAAAACTACCGTATGCACGAGATAGAGTAGTAGAATGTTGCTTTTGGGTTATGACCGTATATTTTGAGCCCCAATATTCTCAAGCAAGGAAAATGATGTCAAAATTTATCTCCATTTTATCATACATTGATGACACATATGATGCATATGGAACTATTGATGAATTAGAACTTTTTACTCAGGCAATTGAAAGGTTAGCACATCATACAATATTATTGTTAAGAATAATTGAATTCGTGACCAAACTTTAGAGATAAAACAATATGAAATTTATCTTTAATCTAGTCATCAAGTTTAGAGTTGGAATTTTTTTAATAATAACTTCAATTTACCCATCTTCAATCTTGGTTGCTAATTCAATTTTTGTTAAGAAATATAGATATTTTCTTCGCATGTATTTCTACACGATATGATTCTTTTAACAATAGAGCAATGGTTAATATTGCAATCAAAATGATTTTTAGGTGGGATATTAATTGCTTGGAGGATCTTCCGGATTACACGAAGTTGCTTTATAAATCACTCTTGGATGTTTATCAAGAGATAGAACAAGAAATGAAGAAAGAAGGAAGAGAATACACCCTTGACCCCTTCTTAAAAGAAGTAAGAGTTTTGCTTTATTGTTAG
Protein sequence

>MS.gene060118.t1

ITSILMRNRFCKEMLQISTLAFGEIFSFLMLQNLWNLIUILRQKLINUKRRLDKCLSQKQRNPWKKSTULTQYVVWVUAIILKTKLIKFWNIFTRVMFKMEKULSKTTFILLLCYLGCUGNMDFTFHLMCSTNSRTSKETSVKHWSEMLRACUTCMKPHIUWFMVKKYWKRRWLLLLVTLSPWPPDUVLLLQHKUNKAUDNLCAKTCLGUWQEVTFPSMSKIHPIMKFYLHWQNWILICSKAYIKRNLETLASGGRSWILLKNYRMHEIEUUNVAFGLUPYILSPNILKQGKUCQNLSPFYHTLMTHMMHMELLMNUNFLLRQLKGGILIAWRIFRITRSCFINHSWMFIKRUNKKURKKEENTPLTPSUKKUEFCFIV