Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062694.t1 | PNY17474.1 | 76.8 | 284 | 62 | 2 | 1 | 280 | 148 | 431 | 4.90E-118 | 434.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062694.t1 | Q9ZUB9 | 38.3 | 264 | 134 | 8 | 8 | 258 | 45 | 292 | 1.5e-35 | 151.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062694.t1 | A0A2K3PQD3 | 76.8 | 284 | 62 | 2 | 1 | 280 | 148 | 431 | 3.5e-118 | 434.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060790 | MS.gene062694 | 0.800725 | 1.26E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062694.t1 | MTR_4g019560 | 79.545 | 264 | 52 | 1 | 9 | 270 | 5 | 268 | 1.05e-153 | 430 |
| MS.gene062694.t1 | MTR_7g057330 | 78.491 | 265 | 54 | 2 | 9 | 270 | 5 | 269 | 1.26e-144 | 407 |
| MS.gene062694.t1 | MTR_1g106020 | 71.769 | 294 | 67 | 4 | 1 | 280 | 137 | 428 | 2.17e-142 | 408 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062694.t1 | AT1G23770 | 39.015 | 264 | 132 | 9 | 8 | 258 | 45 | 292 | 8.30e-38 | 136 |
| MS.gene062694.t1 | AT1G23780 | 40.541 | 222 | 118 | 6 | 8 | 226 | 159 | 369 | 1.96e-31 | 122 |
| MS.gene062694.t1 | AT1G70360 | 34.419 | 215 | 111 | 4 | 3 | 214 | 6 | 193 | 2.05e-30 | 113 |
Find 43 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCATTGTTTGAAGATTT+TGG | 0.078660 | 6.4:-45384901 | MS.gene062694:CDS |
| TTTGACAGCATCAACTTTAA+AGG | 0.157144 | 6.4:+45384832 | None:intergenic |
| TGAACAAGTACAACACATTT+TGG | 0.202260 | 6.4:+45384606 | None:intergenic |
| AAGAAGTTTGAGGAGGAGTT+TGG | 0.230453 | 6.4:-45384544 | MS.gene062694:CDS |
| AAAAGAATTTCTAATCGTTT+TGG | 0.254323 | 6.4:-45384400 | MS.gene062694:CDS |
| TGTTAGCGATTTCAAATTAC+TGG | 0.272293 | 6.4:-45385136 | MS.gene062694:CDS |
| CTATCTTCCTGGTGATGATT+TGG | 0.283249 | 6.4:-45384629 | MS.gene062694:CDS |
| TTAATATTTGACTATCTTCC+TGG | 0.311249 | 6.4:-45384640 | MS.gene062694:CDS |
| TATGGTTGTGAAGAACAATT+TGG | 0.311934 | 6.4:-45385205 | MS.gene062694:CDS |
| TCATACTCTCCACCTCAAAT+TGG | 0.344996 | 6.4:+45384370 | None:intergenic |
| CTTCCTGACATTCTTACTAA+TGG | 0.366226 | 6.4:-45384982 | MS.gene062694:CDS |
| AGAACTTGTATGCTCATTAC+CGG | 0.392077 | 6.4:-45384492 | MS.gene062694:CDS |
| CAGCATCAACTTTAAAGGCT+TGG | 0.408028 | 6.4:+45384837 | None:intergenic |
| ATTGATCTTTGTGATAAAGC+TGG | 0.414028 | 6.4:-45384712 | MS.gene062694:CDS |
| TTTGGAATGGCTCCAATTTG+AGG | 0.439936 | 6.4:-45384382 | None:intergenic |
| ACATTCACAAAATGCCCTAA+TGG | 0.446854 | 6.4:+45384928 | None:intergenic |
| TTTCAAATTACTGGTATTTG+CGG | 0.449872 | 6.4:-45385127 | MS.gene062694:CDS |
| ATTAAGAGCTTAAGTTCCAC+TGG | 0.464446 | 6.4:+45384658 | None:intergenic |
| TCTTTGTGATAAAGCTGGAT+TGG | 0.479011 | 6.4:-45384707 | MS.gene062694:CDS |
| GTACCATTAGTAAGAATGTC+AGG | 0.485867 | 6.4:+45384979 | None:intergenic |
| ACAACTCATCATTTGAAGCT+AGG | 0.492575 | 6.4:+45384572 | None:intergenic |
| GACAAATGCTGAATGGTATG+AGG | 0.494580 | 6.4:-45384522 | MS.gene062694:CDS |
| TGAATCGGGGTTTGTTCGTG+TGG | 0.510242 | 6.4:-45385088 | MS.gene062694:CDS |
| GTGAAGAACAATTTGGAACC+TGG | 0.532138 | 6.4:-45385198 | MS.gene062694:CDS |
| GTTCTGACTTCTTTGTTGCC+CGG | 0.533032 | 6.4:+45384473 | None:intergenic |
| GAGCTTAAGTTCCACTGGGA+GGG | 0.554317 | 6.4:+45384663 | None:intergenic |
| CGAGGAATTCGAAAAGAAAA+CGG | 0.560654 | 6.4:+45384451 | None:intergenic |
| GTCAGGAAGGGTATACCTCA+AGG | 0.570564 | 6.4:+45384996 | None:intergenic |
| GTGGAAGAAGAAGTTTGAGG+AGG | 0.585743 | 6.4:-45384551 | MS.gene062694:CDS |
| TCACAAAGATCAATTAAGAG+TGG | 0.590608 | 6.4:+45384721 | None:intergenic |
| GAGTTTGGACAAATGCTGAA+TGG | 0.590973 | 6.4:-45384529 | MS.gene062694:CDS |
| CATTAGTAAGAATGTCAGGA+AGG | 0.593966 | 6.4:+45384983 | None:intergenic |
| TAGCTTCAAATGATGAGTTG+TGG | 0.596648 | 6.4:-45384570 | MS.gene062694:CDS |
| GTTGTGGAAGAAGAAGTTTG+AGG | 0.602273 | 6.4:-45384554 | MS.gene062694:CDS |
| ATTAGTAAGAATGTCAGGAA+GGG | 0.612924 | 6.4:+45384984 | None:intergenic |
| TCAGGAAGGGTATACCTCAA+GGG | 0.635601 | 6.4:+45384997 | None:intergenic |
| CTAATACAATGATACCCTTG+AGG | 0.638994 | 6.4:-45385011 | MS.gene062694:CDS |
| AGAGCTTAAGTTCCACTGGG+AGG | 0.642707 | 6.4:+45384662 | None:intergenic |
| GAACTTGTATGCTCATTACC+GGG | 0.642737 | 6.4:-45384491 | MS.gene062694:CDS |
| TTAAGAGCTTAAGTTCCACT+GGG | 0.643405 | 6.4:+45384659 | None:intergenic |
| ACTGGGAGGGTCATAAAACA+AGG | 0.668561 | 6.4:+45384676 | None:intergenic |
| GGGAGGGTCATAAAACAAGG+TGG | 0.729265 | 6.4:+45384679 | None:intergenic |
| TATCTCATAATACGACTCCG+AGG | 0.737541 | 6.4:+45384433 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTAAAAATTCAGCCATT+AGG | - | chr6.4:45384645-45384664 | MS.gene062694:CDS | 20.0% |
| !! | ATTTAAAAATTCAGCCATTA+GGG | - | chr6.4:45384646-45384665 | MS.gene062694:CDS | 20.0% |
| !!! | AAAAGAATTTCTAATCGTTT+TGG | - | chr6.4:45385188-45385207 | MS.gene062694:CDS | 20.0% |
| !!! | AGTTTTAATGTTAATGATGA+TGG | - | chr6.4:45384795-45384814 | MS.gene062694:CDS | 20.0% |
| ! | TTAATATTTGACTATCTTCC+TGG | - | chr6.4:45384948-45384967 | MS.gene062694:CDS | 25.0% |
| ! | TTTCAAATTACTGGTATTTG+CGG | - | chr6.4:45384461-45384480 | MS.gene062694:CDS | 25.0% |
| !! | GTTCATTGTTTGAAGATTTT+GGG | - | chr6.4:45384688-45384707 | MS.gene062694:CDS | 25.0% |
| !! | TCATGCTTTTATTCTTGAAT+CGG | - | chr6.4:45384485-45384504 | MS.gene062694:CDS | 25.0% |
| !!! | AATTTCTAATCGTTTTGGAA+TGG | - | chr6.4:45385193-45385212 | MS.gene062694:CDS | 25.0% |
| ATTAGTAAGAATGTCAGGAA+GGG | + | chr6.4:45384607-45384626 | None:intergenic | 30.0% | |
| ATTGATCTTTGTGATAAAGC+TGG | - | chr6.4:45384876-45384895 | MS.gene062694:CDS | 30.0% | |
| TCACAAAGATCAATTAAGAG+TGG | + | chr6.4:45384870-45384889 | None:intergenic | 30.0% | |
| TGTTAGCGATTTCAAATTAC+TGG | - | chr6.4:45384452-45384471 | MS.gene062694:CDS | 30.0% | |
| TTTGACAGCATCAACTTTAA+AGG | + | chr6.4:45384759-45384778 | None:intergenic | 30.0% | |
| ! | ATGCTTTTATTCTTGAATCG+GGG | - | chr6.4:45384487-45384506 | MS.gene062694:CDS | 30.0% |
| ! | CATGCTTTTATTCTTGAATC+GGG | - | chr6.4:45384486-45384505 | MS.gene062694:CDS | 30.0% |
| ! | GAGATGAAGTTTTTGAATTG+TGG | - | chr6.4:45384817-45384836 | MS.gene062694:CDS | 30.0% |
| ! | GGTTCATTGTTTGAAGATTT+TGG | - | chr6.4:45384687-45384706 | MS.gene062694:CDS | 30.0% |
| ! | TGAACAAGTACAACACATTT+TGG | + | chr6.4:45384985-45385004 | None:intergenic | 30.0% |
| !! | TTTTGGGTCAATAGTACATA+AGG | - | chr6.4:45384704-45384723 | MS.gene062694:CDS | 30.0% |
| ACATTCACAAAATGCCCTAA+TGG | + | chr6.4:45384663-45384682 | None:intergenic | 35.0% | |
| AGAACTTGTATGCTCATTAC+CGG | - | chr6.4:45385096-45385115 | MS.gene062694:CDS | 35.0% | |
| ATTAAGAGCTTAAGTTCCAC+TGG | + | chr6.4:45384933-45384952 | None:intergenic | 35.0% | |
| CATTAGTAAGAATGTCAGGA+AGG | + | chr6.4:45384608-45384627 | None:intergenic | 35.0% | |
| CGAGGAATTCGAAAAGAAAA+CGG | + | chr6.4:45385140-45385159 | None:intergenic | 35.0% | |
| CTAATACAATGATACCCTTG+AGG | - | chr6.4:45384577-45384596 | MS.gene062694:CDS | 35.0% | |
| CTTCCTGACATTCTTACTAA+TGG | - | chr6.4:45384606-45384625 | MS.gene062694:CDS | 35.0% | |
| GTACCATTAGTAAGAATGTC+AGG | + | chr6.4:45384612-45384631 | None:intergenic | 35.0% | |
| TAGCTTCAAATGATGAGTTG+TGG | - | chr6.4:45385018-45385037 | MS.gene062694:CDS | 35.0% | |
| TCTTTGTGATAAAGCTGGAT+TGG | - | chr6.4:45384881-45384900 | MS.gene062694:CDS | 35.0% | |
| TTAAGAGCTTAAGTTCCACT+GGG | + | chr6.4:45384932-45384951 | None:intergenic | 35.0% | |
| ! | ACAACTCATCATTTGAAGCT+AGG | + | chr6.4:45385019-45385038 | None:intergenic | 35.0% |
| ! | CGTTTTCTTTTCGAATTCCT+CGG | - | chr6.4:45385138-45385157 | MS.gene062694:CDS | 35.0% |
| !! | CAGTTTTCTAGTATGGTGAT+TGG | - | chr6.4:45384525-45384544 | MS.gene062694:CDS | 35.0% |
| !! | TGTGGATCAGTTTTCTAGTA+TGG | - | chr6.4:45384518-45384537 | MS.gene062694:CDS | 35.0% |
| !!! | GGAACCTGGTTTTGTAAAAA+GGG | - | chr6.4:45384404-45384423 | MS.gene062694:CDS | 35.0% |
| !!! | TGGAACCTGGTTTTGTAAAA+AGG | - | chr6.4:45384403-45384422 | MS.gene062694:CDS | 35.0% |
| AAGAAGTTTGAGGAGGAGTT+TGG | - | chr6.4:45385044-45385063 | MS.gene062694:CDS | 40.0% | |
| GAACTTGTATGCTCATTACC+GGG | - | chr6.4:45385097-45385116 | MS.gene062694:CDS | 40.0% | |
| GACAAATGCTGAATGGTATG+AGG | - | chr6.4:45385066-45385085 | MS.gene062694:CDS | 40.0% | |
| GAGTTTGGACAAATGCTGAA+TGG | - | chr6.4:45385059-45385078 | MS.gene062694:CDS | 40.0% | |
| GTTGTGGAAGAAGAAGTTTG+AGG | - | chr6.4:45385034-45385053 | MS.gene062694:CDS | 40.0% | |
| TATCTCATAATACGACTCCG+AGG | + | chr6.4:45385158-45385177 | None:intergenic | 40.0% | |
| ! | AGCACCCTTTTTACAAAACC+AGG | + | chr6.4:45384411-45384430 | None:intergenic | 40.0% |
| ! | CAGCATCAACTTTAAAGGCT+TGG | + | chr6.4:45384754-45384773 | None:intergenic | 40.0% |
| ! | CTATCTTCCTGGTGATGATT+TGG | - | chr6.4:45384959-45384978 | MS.gene062694:CDS | 40.0% |
| ! | GGGCATTTTGTGAATGTCTA+TGG | - | chr6.4:45384666-45384685 | MS.gene062694:CDS | 40.0% |
| ! | GTGAAGAACAATTTGGAACC+TGG | - | chr6.4:45384390-45384409 | MS.gene062694:CDS | 40.0% |
| !! | ATTTTGGCCAAATCATCACC+AGG | + | chr6.4:45384969-45384988 | None:intergenic | 40.0% |
| ACTGGGAGGGTCATAAAACA+AGG | + | chr6.4:45384915-45384934 | None:intergenic | 45.0% | |
| GTGGAAGAAGAAGTTTGAGG+AGG | - | chr6.4:45385037-45385056 | MS.gene062694:CDS | 45.0% | |
| GTTCTGACTTCTTTGTTGCC+CGG | + | chr6.4:45385118-45385137 | None:intergenic | 45.0% | |
| ! | TCAGGAAGGGTATACCTCAA+GGG | + | chr6.4:45384594-45384613 | None:intergenic | 45.0% |
| !! | TTGTTTTATGACCCTCCCAG+TGG | - | chr6.4:45384914-45384933 | MS.gene062694:CDS | 45.0% |
| AGAGCTTAAGTTCCACTGGG+AGG | + | chr6.4:45384929-45384948 | None:intergenic | 50.0% | |
| GAGCTTAAGTTCCACTGGGA+GGG | + | chr6.4:45384928-45384947 | None:intergenic | 50.0% | |
| GGGAGGGTCATAAAACAAGG+TGG | + | chr6.4:45384912-45384931 | None:intergenic | 50.0% | |
| ! | GTCAGGAAGGGTATACCTCA+AGG | + | chr6.4:45384595-45384614 | None:intergenic | 50.0% |
| ! | TGAATCGGGGTTTGTTCGTG+TGG | - | chr6.4:45384500-45384519 | MS.gene062694:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 45384384 | 45385226 | 45384384 | ID=MS.gene062694 |
| chr6.4 | mRNA | 45384384 | 45385226 | 45384384 | ID=MS.gene062694.t1;Parent=MS.gene062694 |
| chr6.4 | exon | 45384384 | 45385226 | 45384384 | ID=MS.gene062694.t1.exon1;Parent=MS.gene062694.t1 |
| chr6.4 | CDS | 45384384 | 45385226 | 45384384 | ID=cds.MS.gene062694.t1;Parent=MS.gene062694.t1 |
| Gene Sequence |
| Protein sequence |
