Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060790.t1 | XP_013455252.1 | 95.6 | 319 | 8 | 1 | 1 | 319 | 1 | 313 | 4.60E-171 | 610.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060790.t1 | Q84KK6 | 79.6 | 323 | 62 | 2 | 1 | 319 | 1 | 323 | 1.8e-147 | 523.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060790.t1 | A0A072UJL0 | 95.6 | 319 | 8 | 1 | 1 | 319 | 1 | 313 | 3.3e-171 | 610.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060790 | MS.gene062682 | 0.804634 | 1.97E-49 | -1.69E-46 |
| MS.gene060790 | MS.gene062694 | 0.800725 | 1.26E-48 | -1.69E-46 |
| MS.gene060790 | MS.gene068442 | 0.80129 | 9.65E-49 | -1.69E-46 |
| MS.gene060790 | MS.gene48635 | 0.835294 | 1.82E-56 | -1.69E-46 |
| MS.gene060790 | MS.gene80468 | 0.801282 | 9.69E-49 | -1.69E-46 |
| MS.gene060790 | MS.gene81503 | 0.846357 | 2.29E-59 | -1.69E-46 |
| MS.gene060790 | MS.gene81504 | 0.821387 | 4.15E-53 | -1.69E-46 |
| MS.gene060790 | MS.gene81505 | 0.837303 | 5.63E-57 | -1.69E-46 |
| MS.gene060790 | MS.gene81506 | 0.843262 | 1.56E-58 | -1.69E-46 |
| MS.gene060790 | MS.gene88261 | 0.810055 | 1.39E-50 | -1.69E-46 |
| MS.gene060790 | MS.gene88286 | 0.907027 | 7.89E-81 | -1.69E-46 |
| MS.gene060790 | MS.gene93899 | 0.836007 | 1.20E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060790.t1 | MTR_4g033085 | 95.312 | 320 | 9 | 1 | 1 | 320 | 1 | 314 | 0.0 | 622 |
| MS.gene060790.t1 | MTR_4g033170 | 94.688 | 320 | 11 | 1 | 1 | 320 | 1 | 314 | 0.0 | 619 |
| MS.gene060790.t1 | MTR_4g033200 | 76.489 | 319 | 73 | 2 | 1 | 318 | 1 | 318 | 0.0 | 513 |
| MS.gene060790.t1 | MTR_4g033015 | 76.176 | 319 | 74 | 2 | 1 | 318 | 1 | 318 | 0.0 | 510 |
| MS.gene060790.t1 | MTR_4g088200 | 76.489 | 319 | 73 | 1 | 1 | 319 | 1 | 317 | 0.0 | 508 |
| MS.gene060790.t1 | MTR_4g033150 | 74.608 | 319 | 79 | 2 | 1 | 318 | 1 | 318 | 8.14e-179 | 499 |
| MS.gene060790.t1 | MTR_4g088190 | 76.324 | 321 | 75 | 1 | 1 | 320 | 1 | 321 | 2.38e-169 | 475 |
| MS.gene060790.t1 | MTR_0794s0020 | 74.000 | 250 | 63 | 2 | 1 | 249 | 1 | 249 | 1.97e-137 | 390 |
| MS.gene060790.t1 | MTR_7g014570 | 48.113 | 318 | 155 | 3 | 6 | 323 | 6 | 313 | 3.74e-105 | 312 |
| MS.gene060790.t1 | MTR_7g014510 | 46.875 | 320 | 165 | 3 | 6 | 325 | 6 | 320 | 2.66e-103 | 307 |
| MS.gene060790.t1 | MTR_1g076940 | 48.428 | 318 | 157 | 5 | 5 | 319 | 5 | 318 | 1.30e-100 | 301 |
| MS.gene060790.t1 | MTR_4g088345 | 48.287 | 321 | 157 | 4 | 1 | 320 | 1 | 313 | 7.46e-97 | 291 |
| MS.gene060790.t1 | MTR_7g024500 | 44.828 | 319 | 164 | 4 | 6 | 324 | 5 | 311 | 6.10e-96 | 288 |
| MS.gene060790.t1 | MTR_7g024520 | 45.141 | 319 | 163 | 4 | 6 | 324 | 5 | 311 | 2.20e-94 | 284 |
| MS.gene060790.t1 | MTR_5g074600 | 45.079 | 315 | 165 | 5 | 11 | 323 | 11 | 319 | 7.49e-93 | 281 |
| MS.gene060790.t1 | MTR_5g074680 | 43.731 | 327 | 173 | 6 | 3 | 324 | 1 | 321 | 7.61e-93 | 281 |
| MS.gene060790.t1 | MTR_5g074580 | 42.767 | 318 | 174 | 5 | 4 | 319 | 5 | 316 | 3.09e-86 | 264 |
| MS.gene060790.t1 | MTR_4g088340 | 46.980 | 298 | 149 | 4 | 24 | 320 | 1 | 290 | 1.14e-85 | 261 |
| MS.gene060790.t1 | MTR_5g074610 | 41.424 | 309 | 148 | 3 | 11 | 319 | 7 | 282 | 2.09e-81 | 250 |
| MS.gene060790.t1 | MTR_5g074540 | 40.514 | 311 | 159 | 5 | 11 | 319 | 11 | 297 | 2.34e-81 | 251 |
| MS.gene060790.t1 | MTR_4g079160 | 40.968 | 310 | 174 | 3 | 11 | 319 | 10 | 311 | 4.84e-80 | 248 |
| MS.gene060790.t1 | MTR_1g076930 | 45.267 | 243 | 124 | 5 | 24 | 263 | 1 | 237 | 2.01e-61 | 196 |
| MS.gene060790.t1 | MTR_3g083620 | 34.528 | 307 | 185 | 9 | 20 | 323 | 27 | 320 | 8.75e-47 | 162 |
| MS.gene060790.t1 | MTR_0017s0100 | 32.288 | 319 | 205 | 6 | 3 | 319 | 14 | 323 | 5.30e-45 | 157 |
| MS.gene060790.t1 | MTR_4g038440 | 31.975 | 319 | 206 | 6 | 3 | 319 | 14 | 323 | 3.87e-44 | 155 |
| MS.gene060790.t1 | MTR_0079s0070 | 32.203 | 295 | 189 | 6 | 27 | 319 | 4 | 289 | 5.16e-40 | 144 |
| MS.gene060790.t1 | MTR_5g098170 | 34.201 | 269 | 163 | 9 | 29 | 290 | 31 | 292 | 1.68e-36 | 135 |
| MS.gene060790.t1 | MTR_3g092900 | 31.988 | 322 | 186 | 10 | 9 | 318 | 17 | 317 | 2.91e-33 | 126 |
| MS.gene060790.t1 | MTR_1g096310 | 30.556 | 324 | 201 | 9 | 5 | 318 | 17 | 326 | 4.17e-33 | 126 |
| MS.gene060790.t1 | MTR_0591s0010 | 30.961 | 281 | 182 | 7 | 26 | 301 | 41 | 314 | 2.03e-32 | 124 |
| MS.gene060790.t1 | MTR_7g012090 | 30.325 | 277 | 176 | 7 | 32 | 298 | 40 | 309 | 3.93e-32 | 123 |
| MS.gene060790.t1 | MTR_7g118320 | 31.852 | 270 | 175 | 6 | 26 | 290 | 26 | 291 | 1.64e-31 | 121 |
| MS.gene060790.t1 | MTR_7g012070 | 30.325 | 277 | 176 | 7 | 32 | 298 | 40 | 309 | 2.79e-31 | 121 |
| MS.gene060790.t1 | MTR_8g068870 | 29.097 | 299 | 200 | 7 | 5 | 298 | 6 | 297 | 4.45e-31 | 120 |
| MS.gene060790.t1 | MTR_7g011900 | 30.686 | 277 | 180 | 7 | 32 | 303 | 40 | 309 | 1.12e-30 | 119 |
| MS.gene060790.t1 | MTR_7g118300 | 30.686 | 277 | 179 | 8 | 26 | 298 | 42 | 309 | 1.64e-30 | 119 |
| MS.gene060790.t1 | MTR_7g012040 | 29.520 | 271 | 180 | 7 | 32 | 298 | 39 | 302 | 2.97e-30 | 118 |
| MS.gene060790.t1 | MTR_1g036490 | 29.866 | 298 | 191 | 8 | 24 | 312 | 29 | 317 | 5.69e-30 | 117 |
| MS.gene060790.t1 | MTR_8g068690 | 30.249 | 281 | 184 | 7 | 26 | 301 | 40 | 313 | 5.88e-30 | 117 |
| MS.gene060790.t1 | MTR_1g036460 | 29.392 | 296 | 195 | 7 | 24 | 312 | 29 | 317 | 1.06e-29 | 117 |
| MS.gene060790.t1 | MTR_8g068750 | 29.617 | 287 | 190 | 7 | 26 | 307 | 41 | 320 | 6.86e-29 | 115 |
| MS.gene060790.t1 | MTR_7g012020 | 29.779 | 272 | 179 | 7 | 32 | 298 | 39 | 303 | 9.03e-29 | 114 |
| MS.gene060790.t1 | MTR_2g072790 | 32.997 | 297 | 176 | 9 | 31 | 319 | 27 | 308 | 1.64e-28 | 113 |
| MS.gene060790.t1 | MTR_4g038440 | 29.658 | 263 | 177 | 4 | 3 | 263 | 14 | 270 | 2.36e-28 | 111 |
| MS.gene060790.t1 | MTR_7g011990 | 27.240 | 279 | 190 | 7 | 32 | 306 | 40 | 309 | 2.41e-28 | 113 |
| MS.gene060790.t1 | MTR_2g055940 | 29.856 | 278 | 174 | 9 | 32 | 300 | 34 | 299 | 1.21e-27 | 111 |
| MS.gene060790.t1 | MTR_8g068570 | 30.357 | 280 | 158 | 8 | 26 | 301 | 41 | 287 | 5.86e-27 | 108 |
| MS.gene060790.t1 | MTR_8g024160 | 33.668 | 199 | 124 | 4 | 101 | 298 | 71 | 262 | 1.15e-25 | 105 |
| MS.gene060790.t1 | MTR_3g021440 | 29.492 | 295 | 197 | 7 | 28 | 318 | 38 | 325 | 1.56e-25 | 105 |
| MS.gene060790.t1 | MTR_1g036510 | 28.188 | 298 | 194 | 9 | 25 | 312 | 44 | 331 | 1.75e-24 | 102 |
| MS.gene060790.t1 | MTR_3g021430 | 28.912 | 294 | 192 | 9 | 32 | 318 | 29 | 312 | 8.20e-23 | 97.8 |
| MS.gene060790.t1 | MTR_6g078850 | 25.952 | 289 | 198 | 7 | 9 | 290 | 33 | 312 | 9.77e-23 | 99.0 |
| MS.gene060790.t1 | MTR_7g012020 | 28.631 | 241 | 163 | 6 | 32 | 267 | 39 | 275 | 8.05e-22 | 94.0 |
| MS.gene060790.t1 | MTR_3g096050 | 27.213 | 305 | 198 | 11 | 23 | 319 | 24 | 312 | 3.52e-20 | 90.5 |
| MS.gene060790.t1 | MTR_8g068730 | 29.259 | 270 | 150 | 7 | 26 | 290 | 41 | 274 | 9.25e-20 | 89.0 |
| MS.gene060790.t1 | MTR_3g096070 | 29.213 | 178 | 120 | 3 | 143 | 319 | 117 | 289 | 2.97e-18 | 84.7 |
| MS.gene060790.t1 | MTR_8g068690 | 26.829 | 246 | 171 | 6 | 26 | 266 | 40 | 281 | 6.63e-18 | 82.8 |
| MS.gene060790.t1 | MTR_8g068740 | 27.737 | 274 | 155 | 8 | 26 | 292 | 41 | 278 | 9.23e-17 | 79.7 |
| MS.gene060790.t1 | MTR_8g068600 | 26.293 | 232 | 162 | 6 | 38 | 264 | 27 | 254 | 4.99e-16 | 77.4 |
| MS.gene060790.t1 | MTR_1g036510 | 27.711 | 249 | 163 | 8 | 25 | 263 | 44 | 285 | 3.98e-15 | 75.1 |
| MS.gene060790.t1 | MTR_8g068620 | 33.929 | 112 | 72 | 1 | 155 | 266 | 4 | 113 | 3.89e-14 | 68.6 |
| MS.gene060790.t1 | MTR_7g012020 | 28.571 | 210 | 141 | 6 | 32 | 236 | 39 | 244 | 5.48e-14 | 71.2 |
| MS.gene060790.t1 | MTR_4g025570 | 37.255 | 102 | 54 | 3 | 6 | 107 | 16 | 107 | 5.17e-13 | 67.8 |
| MS.gene060790.t1 | MTR_1g050220 | 33.613 | 119 | 66 | 2 | 194 | 312 | 147 | 252 | 2.90e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060790.t1 | AT5G54160 | 32.414 | 290 | 186 | 6 | 31 | 318 | 34 | 315 | 5.25e-36 | 134 |
| MS.gene060790.t1 | AT4G35150 | 30.573 | 314 | 159 | 9 | 16 | 319 | 15 | 279 | 5.48e-35 | 130 |
| MS.gene060790.t1 | AT1G77520 | 33.813 | 278 | 167 | 8 | 31 | 298 | 44 | 314 | 9.18e-34 | 128 |
| MS.gene060790.t1 | AT4G35160 | 31.481 | 324 | 194 | 10 | 16 | 323 | 29 | 340 | 9.18e-33 | 125 |
| MS.gene060790.t1 | AT3G53140 | 31.788 | 302 | 178 | 10 | 31 | 319 | 27 | 313 | 1.86e-29 | 116 |
| MS.gene060790.t1 | AT1G63140 | 29.545 | 308 | 197 | 9 | 9 | 303 | 39 | 339 | 1.02e-26 | 109 |
| MS.gene060790.t1 | AT1G63140 | 29.545 | 308 | 197 | 9 | 9 | 303 | 19 | 319 | 1.07e-26 | 108 |
| MS.gene060790.t1 | AT5G53810 | 31.915 | 282 | 174 | 7 | 29 | 299 | 38 | 312 | 1.38e-26 | 108 |
| MS.gene060790.t1 | AT1G77530 | 32.258 | 279 | 172 | 7 | 31 | 299 | 44 | 315 | 3.86e-26 | 107 |
| MS.gene060790.t1 | AT1G33030 | 27.797 | 295 | 197 | 8 | 31 | 319 | 21 | 305 | 3.95e-26 | 107 |
| MS.gene060790.t1 | AT1G51990 | 28.819 | 288 | 188 | 8 | 33 | 315 | 32 | 307 | 1.21e-25 | 105 |
| MS.gene060790.t1 | AT1G51990 | 28.819 | 288 | 188 | 8 | 33 | 315 | 32 | 307 | 1.30e-24 | 102 |
| MS.gene060790.t1 | AT1G76790 | 27.190 | 331 | 202 | 11 | 7 | 319 | 9 | 318 | 7.36e-20 | 89.7 |
| MS.gene060790.t1 | AT1G62900 | 36.641 | 131 | 80 | 1 | 173 | 303 | 16 | 143 | 2.88e-19 | 85.1 |
| MS.gene060790.t1 | AT5G37170 | 30.000 | 150 | 100 | 3 | 172 | 319 | 43 | 189 | 6.30e-16 | 76.3 |
| MS.gene060790.t1 | AT5G37170 | 30.000 | 150 | 100 | 3 | 172 | 319 | 143 | 289 | 1.09e-15 | 77.4 |
| MS.gene060790.t1 | AT1G21130 | 24.834 | 302 | 199 | 9 | 31 | 319 | 40 | 326 | 4.11e-15 | 75.9 |
| MS.gene060790.t1 | AT5G37170 | 32.824 | 131 | 80 | 4 | 194 | 319 | 14 | 141 | 5.10e-15 | 72.8 |
| MS.gene060790.t1 | AT1G21120 | 24.503 | 302 | 200 | 9 | 31 | 319 | 88 | 374 | 5.66e-15 | 75.9 |
| MS.gene060790.t1 | AT1G21120 | 24.503 | 302 | 200 | 9 | 31 | 319 | 40 | 326 | 5.71e-15 | 75.5 |
| MS.gene060790.t1 | AT1G21100 | 24.834 | 302 | 199 | 9 | 31 | 319 | 40 | 326 | 1.08e-14 | 74.7 |
| MS.gene060790.t1 | AT1G21110 | 23.510 | 302 | 203 | 9 | 31 | 319 | 40 | 326 | 3.06e-14 | 73.2 |
| MS.gene060790.t1 | AT1G63140 | 27.239 | 268 | 178 | 8 | 9 | 263 | 39 | 302 | 3.43e-14 | 72.4 |
| MS.gene060790.t1 | AT1G33030 | 26.360 | 239 | 163 | 7 | 31 | 263 | 21 | 252 | 2.13e-13 | 69.7 |
| MS.gene060790.t1 | AT1G33030 | 26.360 | 239 | 163 | 7 | 31 | 263 | 21 | 252 | 4.08e-13 | 68.9 |
Find 62 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATACATGTTTGTATAAA+TGG | 0.137435 | 4.4:+70583885 | None:intergenic |
| TTCAAAGATGAGGCTAATTC+AGG | 0.265492 | 4.4:+70583776 | None:intergenic |
| CAGCCATGTGTTTGAGGGTT+TGG | 0.284838 | 4.4:-70583363 | MS.gene060790:CDS |
| TATAGGACATTAACTTTAGA+AGG | 0.302242 | 4.4:+70583746 | None:intergenic |
| GTTTGAGGGTTTGGAGTCTT+TGG | 0.303653 | 4.4:-70583354 | MS.gene060790:CDS |
| CTCAGAGAACCATTTCTTAG+AGG | 0.305502 | 4.4:+70583532 | None:intergenic |
| AACAGCAAAGAAGCTATTTC+AGG | 0.318216 | 4.4:-70582529 | MS.gene060790:CDS |
| GTTGATGTGGCAGGTGGGAC+TGG | 0.337992 | 4.4:-70583332 | MS.gene060790:CDS |
| CTAAAGTTAATGTCCTATAT+AGG | 0.375731 | 4.4:-70583742 | MS.gene060790:CDS |
| AAAGAAGCTATTTCAGGAAA+AGG | 0.388596 | 4.4:-70582523 | MS.gene060790:CDS |
| CAGGTTGTGGCTAACTTATC+TGG | 0.396383 | 4.4:-70583245 | MS.gene060790:CDS |
| CCACTGACGAGAAGCTTTGA+AGG | 0.398681 | 4.4:+70583623 | None:intergenic |
| AATTCTTCATCCATGTTCTC+TGG | 0.398747 | 4.4:-70583567 | MS.gene060790:CDS |
| TCATAGGCTTTCCATGTTTG+TGG | 0.399025 | 4.4:+70583804 | None:intergenic |
| GATAAACTTAGTTTGTTTCA+AGG | 0.401061 | 4.4:-70583434 | MS.gene060790:CDS |
| CAAAATTTGAACTTTGTTGG+TGG | 0.427047 | 4.4:-70583218 | MS.gene060790:CDS |
| ACTCAAAATTTGAACTTTGT+TGG | 0.429435 | 4.4:-70583221 | MS.gene060790:CDS |
| GCTAATTCAGGAACAGTCAT+AGG | 0.442962 | 4.4:+70583788 | None:intergenic |
| AATGTTCAAGCTTGCTCTCA+AGG | 0.450410 | 4.4:-70583390 | MS.gene060790:CDS |
| TGTGGCACTCCTCTAAGAAA+TGG | 0.452397 | 4.4:-70583541 | MS.gene060790:CDS |
| GATTGCAGCCATGTGTTTGA+GGG | 0.455193 | 4.4:-70583368 | MS.gene060790:CDS |
| ATAAACTTAGTTTGTTTCAA+GGG | 0.456335 | 4.4:-70583433 | MS.gene060790:CDS |
| ACAACGGTGAAAGGCAAAGA+AGG | 0.456389 | 4.4:-70583680 | MS.gene060790:CDS |
| GCAAGCGCAAGAACCTATAT+AGG | 0.464145 | 4.4:+70583729 | None:intergenic |
| AGAGACTTGAAGCCACATAC+TGG | 0.465897 | 4.4:+70582316 | None:intergenic |
| GGATTGCAGCCATGTGTTTG+AGG | 0.478258 | 4.4:-70583369 | MS.gene060790:CDS |
| GAGTCTTTGGTTGATGTGGC+AGG | 0.479885 | 4.4:-70583341 | MS.gene060790:CDS |
| AAGAAGCTATTTCAGGAAAA+GGG | 0.484470 | 4.4:-70582522 | MS.gene060790:CDS |
| GCACTCTATGAAAGTGCAAT+GGG | 0.484567 | 4.4:-70583497 | MS.gene060790:CDS |
| ATCCATGTTCTCTGGATATG+TGG | 0.500274 | 4.4:-70583559 | MS.gene060790:CDS |
| TCTTTGGTTGATGTGGCAGG+TGG | 0.504535 | 4.4:-70583338 | MS.gene060790:CDS |
| CTCAAATCAGCTGTGGAACT+AGG | 0.506689 | 4.4:-70583842 | MS.gene060790:CDS |
| TTTAGATCAACCACAGGTTG+TGG | 0.518679 | 4.4:-70583258 | MS.gene060790:CDS |
| CACAGCTGATTTGAGTGCCA+TGG | 0.520024 | 4.4:+70583850 | None:intergenic |
| TGCACTCTATGAAAGTGCAA+TGG | 0.543647 | 4.4:-70583498 | MS.gene060790:CDS |
| CAACGGTGAAAGGCAAAGAA+GGG | 0.548081 | 4.4:-70583679 | MS.gene060790:CDS |
| GAAATGGTTCTCTGAGGAGA+AGG | 0.549990 | 4.4:-70583525 | MS.gene060790:CDS |
| TTTGGAGTCTTTGGTTGATG+TGG | 0.555368 | 4.4:-70583345 | MS.gene060790:CDS |
| TTGAGTAAAACAGCATCTGC+AGG | 0.557051 | 4.4:+70583173 | None:intergenic |
| TACAAGATTACTCCAGTATG+TGG | 0.560046 | 4.4:-70582328 | MS.gene060790:CDS |
| AGGATAAAGTTTCAAAGATG+AGG | 0.566162 | 4.4:+70583766 | None:intergenic |
| TAAACTTAGTTTGTTTCAAG+GGG | 0.566712 | 4.4:-70583432 | MS.gene060790:CDS |
| CAAATGGATTTCAAGAGCAA+TGG | 0.577950 | 4.4:-70583941 | MS.gene060790:CDS |
| CTTTGGTTGATGTGGCAGGT+GGG | 0.578821 | 4.4:-70583337 | MS.gene060790:CDS |
| GTTGCAATTGCACTTTGACA+TGG | 0.578956 | 4.4:-70582431 | MS.gene060790:intron |
| TGCCACATATCCAGAGAACA+TGG | 0.593078 | 4.4:+70583557 | None:intergenic |
| CATTTGTGAATCAGCTGCCA+TGG | 0.594995 | 4.4:+70583409 | None:intergenic |
| GTCAAAGTGCAATTGCAACT+CGG | 0.597693 | 4.4:+70582435 | None:intergenic |
| TAGTTTGTTTCAAGGGGCCA+TGG | 0.599443 | 4.4:-70583426 | MS.gene060790:CDS |
| CCTTCAAAGCTTCTCGTCAG+TGG | 0.601067 | 4.4:-70583623 | MS.gene060790:CDS |
| GATAAGTTAGCCACAACCTG+TGG | 0.609880 | 4.4:+70583248 | None:intergenic |
| AGTAAAACAGCATCTGCAGG+AGG | 0.624714 | 4.4:+70583176 | None:intergenic |
| GTAGATGCAATCCACAAACA+TGG | 0.627357 | 4.4:-70583815 | MS.gene060790:CDS |
| CATGGCACTCAAATCAGCTG+TGG | 0.632130 | 4.4:-70583849 | MS.gene060790:CDS |
| ACTCCAAACCCTCAAACACA+TGG | 0.632186 | 4.4:+70583360 | None:intergenic |
| AACGGTGAAAGGCAAAGAAG+GGG | 0.637209 | 4.4:-70583678 | MS.gene060790:CDS |
| TTGCGCTTGCTAACACACGA+TGG | 0.651331 | 4.4:-70583716 | MS.gene060790:CDS |
| AAAACTGTGCATTTGATGTG+AGG | 0.654544 | 4.4:+70583278 | None:intergenic |
| TGAAGAATTCCCTTAACAAG+TGG | 0.661082 | 4.4:+70583581 | None:intergenic |
| CTCTAAGAAATGGTTCTCTG+AGG | 0.677453 | 4.4:-70583531 | MS.gene060790:CDS |
| GTAAAACAGCATCTGCAGGA+GGG | 0.681270 | 4.4:+70583177 | None:intergenic |
| CTGACGAGAAGCTTTGAAGG+AGG | 0.706966 | 4.4:+70583626 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATATTATTTTAATTTAT+TGG | - | chr4.4:70583608-70583627 | MS.gene060790:CDS | 0.0% |
| !! | TTTGTTAAGAAAGTTAAAAA+AGG | + | chr4.4:70582783-70582802 | None:intergenic | 15.0% |
| !! | CATATATGGACAAAATAAAA+TGG | + | chr4.4:70583141-70583160 | None:intergenic | 20.0% |
| !! | TATGAAATCATGAACATATA+TGG | + | chr4.4:70583155-70583174 | None:intergenic | 20.0% |
| !! | TGTATACATGTTTGTATAAA+TGG | + | chr4.4:70582367-70582386 | None:intergenic | 20.0% |
| !!! | ATAAACTTAGTTTGTTTCAA+GGG | - | chr4.4:70582816-70582835 | MS.gene060790:intron | 20.0% |
| !!! | ATCAAGAAATTTTTACTTGT+GGG | - | chr4.4:70583187-70583206 | MS.gene060790:CDS | 20.0% |
| !!! | TATCAAGAAATTTTTACTTG+TGG | - | chr4.4:70583186-70583205 | MS.gene060790:CDS | 20.0% |
| ! | AAGAAAGAGAAAAGAAAGAA+TGG | - | chr4.4:70583865-70583884 | MS.gene060790:CDS | 25.0% |
| ! | ACTCAAAATTTGAACTTTGT+TGG | - | chr4.4:70583028-70583047 | MS.gene060790:intron | 25.0% |
| ! | ATGTTCATGATTTCATAATC+AGG | - | chr4.4:70583158-70583177 | MS.gene060790:intron | 25.0% |
| ! | CTAAAGTTAATGTCCTATAT+AGG | - | chr4.4:70582507-70582526 | MS.gene060790:CDS | 25.0% |
| ! | TATAGGACATTAACTTTAGA+AGG | + | chr4.4:70582506-70582525 | None:intergenic | 25.0% |
| !! | TCAAGAAATTTTTACTTGTG+GGG | - | chr4.4:70583188-70583207 | MS.gene060790:CDS | 25.0% |
| !!! | ATACAGTTTTATAAGTTCCA+TGG | - | chr4.4:70582382-70582401 | MS.gene060790:intron | 25.0% |
| !!! | GATAAACTTAGTTTGTTTCA+AGG | - | chr4.4:70582815-70582834 | MS.gene060790:intron | 25.0% |
| !!! | TAAACTTAGTTTGTTTCAAG+GGG | - | chr4.4:70582817-70582836 | MS.gene060790:intron | 25.0% |
| AGGATAAAGTTTCAAAGATG+AGG | + | chr4.4:70582486-70582505 | None:intergenic | 30.0% | |
| ATCTCAAATGTCTTTCAAAG+TGG | - | chr4.4:70583652-70583671 | MS.gene060790:CDS | 30.0% | |
| ATTTCAGGAAAAGGGAAAAA+AGG | - | chr4.4:70583735-70583754 | MS.gene060790:CDS | 30.0% | |
| CAAAATTTGAACTTTGTTGG+TGG | - | chr4.4:70583031-70583050 | MS.gene060790:intron | 30.0% | |
| CTTAACTCAAAAAGTTACGA+CGG | + | chr4.4:70583537-70583556 | None:intergenic | 30.0% | |
| TCTCAAATGTCTTTCAAAGT+GGG | - | chr4.4:70583653-70583672 | MS.gene060790:CDS | 30.0% | |
| ! | AAAGAAGCTATTTCAGGAAA+AGG | - | chr4.4:70583726-70583745 | MS.gene060790:CDS | 30.0% |
| ! | AAGAAGCTATTTCAGGAAAA+GGG | - | chr4.4:70583727-70583746 | MS.gene060790:CDS | 30.0% |
| AAAACTGTGCATTTGATGTG+AGG | + | chr4.4:70582974-70582993 | None:intergenic | 35.0% | |
| AAGAAACTCATATATGACGC+AGG | - | chr4.4:70583888-70583907 | MS.gene060790:CDS | 35.0% | |
| AATTCTTCATCCATGTTCTC+TGG | - | chr4.4:70582682-70582701 | MS.gene060790:intron | 35.0% | |
| GTAATGATGACTCTGCATAA+TGG | - | chr4.4:70583840-70583859 | MS.gene060790:CDS | 35.0% | |
| TACAAGATTACTCCAGTATG+TGG | - | chr4.4:70583921-70583940 | MS.gene060790:CDS | 35.0% | |
| TGAAGAATTCCCTTAACAAG+TGG | + | chr4.4:70582671-70582690 | None:intergenic | 35.0% | |
| TGGTTTCTTCGCAAAAACAA+CGG | - | chr4.4:70582553-70582572 | MS.gene060790:CDS | 35.0% | |
| TTCAAAGATGAGGCTAATTC+AGG | + | chr4.4:70582476-70582495 | None:intergenic | 35.0% | |
| ! | AACAGCAAAGAAGCTATTTC+AGG | - | chr4.4:70583720-70583739 | MS.gene060790:CDS | 35.0% |
| !! | AAAGTGGGTTTTGCATGATT+GGG | - | chr4.4:70583668-70583687 | MS.gene060790:CDS | 35.0% |
| !! | ATGTTTTGCACCACTTGTTA+AGG | - | chr4.4:70582658-70582677 | MS.gene060790:intron | 35.0% |
| !! | TGTTTTGCACCACTTGTTAA+GGG | - | chr4.4:70582659-70582678 | MS.gene060790:intron | 35.0% |
| ATCCAGTCACATGTGATTGA+GGG | + | chr4.4:70583491-70583510 | None:intergenic | 40.0% | |
| ATCCATGTTCTCTGGATATG+TGG | - | chr4.4:70582690-70582709 | MS.gene060790:intron | 40.0% | |
| CTCAGAGAACCATTTCTTAG+AGG | + | chr4.4:70582720-70582739 | None:intergenic | 40.0% | |
| GCTAATTCAGGAACAGTCAT+AGG | + | chr4.4:70582464-70582483 | None:intergenic | 40.0% | |
| GTAGATGCAATCCACAAACA+TGG | - | chr4.4:70582434-70582453 | MS.gene060790:CDS | 40.0% | |
| GTCAAAGTGCAATTGCAACT+CGG | + | chr4.4:70583817-70583836 | None:intergenic | 40.0% | |
| GTTGCAATTGCACTTTGACA+TGG | - | chr4.4:70583818-70583837 | MS.gene060790:CDS | 40.0% | |
| TCATAGGCTTTCCATGTTTG+TGG | + | chr4.4:70582448-70582467 | None:intergenic | 40.0% | |
| TTCGCAAAAACAACGGTGAA+AGG | - | chr4.4:70582560-70582579 | MS.gene060790:CDS | 40.0% | |
| TTGAGTAAAACAGCATCTGC+AGG | + | chr4.4:70583079-70583098 | None:intergenic | 40.0% | |
| TTTAGATCAACCACAGGTTG+TGG | - | chr4.4:70582991-70583010 | MS.gene060790:intron | 40.0% | |
| ! | AATGTTCAAGCTTGCTCTCA+AGG | - | chr4.4:70582859-70582878 | MS.gene060790:intron | 40.0% |
| ! | ATTGAGGGCACATACATAGA+GGG | + | chr4.4:70583476-70583495 | None:intergenic | 40.0% |
| ! | CTCTAAGAAATGGTTCTCTG+AGG | - | chr4.4:70582718-70582737 | MS.gene060790:intron | 40.0% |
| ! | GCACTCTATGAAAGTGCAAT+GGG | - | chr4.4:70582752-70582771 | MS.gene060790:intron | 40.0% |
| ! | TGCACTCTATGAAAGTGCAA+TGG | - | chr4.4:70582751-70582770 | MS.gene060790:intron | 40.0% |
| ! | TTTGGAGTCTTTGGTTGATG+TGG | - | chr4.4:70582904-70582923 | MS.gene060790:intron | 40.0% |
| !! | CAAAGTGGGTTTTGCATGAT+TGG | - | chr4.4:70583667-70583686 | MS.gene060790:CDS | 40.0% |
| !!! | CACAGTTTTAGATCAACCAC+AGG | - | chr4.4:70582985-70583004 | MS.gene060790:intron | 40.0% |
| !!! | TGCAGATGCTGTTTTACTCA+AGG | - | chr4.4:70583078-70583097 | MS.gene060790:intron | 40.0% |
| AACGGTGAAAGGCAAAGAAG+GGG | - | chr4.4:70582571-70582590 | MS.gene060790:CDS | 45.0% | |
| ACAACGGTGAAAGGCAAAGA+AGG | - | chr4.4:70582569-70582588 | MS.gene060790:CDS | 45.0% | |
| ACTCCAAACCCTCAAACACA+TGG | + | chr4.4:70582892-70582911 | None:intergenic | 45.0% | |
| AGAGACTTGAAGCCACATAC+TGG | + | chr4.4:70583936-70583955 | None:intergenic | 45.0% | |
| AGTAAAACAGCATCTGCAGG+AGG | + | chr4.4:70583076-70583095 | None:intergenic | 45.0% | |
| CAACGGTGAAAGGCAAAGAA+GGG | - | chr4.4:70582570-70582589 | MS.gene060790:CDS | 45.0% | |
| CAGGTTGTGGCTAACTTATC+TGG | - | chr4.4:70583004-70583023 | MS.gene060790:intron | 45.0% | |
| CATTTGTGAATCAGCTGCCA+TGG | + | chr4.4:70582843-70582862 | None:intergenic | 45.0% | |
| CTCAAATCAGCTGTGGAACT+AGG | - | chr4.4:70582407-70582426 | MS.gene060790:intron | 45.0% | |
| GATAAGTTAGCCACAACCTG+TGG | + | chr4.4:70583004-70583023 | None:intergenic | 45.0% | |
| GATCCAGTCACATGTGATTG+AGG | + | chr4.4:70583492-70583511 | None:intergenic | 45.0% | |
| GATTGCAGCCATGTGTTTGA+GGG | - | chr4.4:70582881-70582900 | MS.gene060790:intron | 45.0% | |
| GCAAGCGCAAGAACCTATAT+AGG | + | chr4.4:70582523-70582542 | None:intergenic | 45.0% | |
| GTAAAACAGCATCTGCAGGA+GGG | + | chr4.4:70583075-70583094 | None:intergenic | 45.0% | |
| TGCCACATATCCAGAGAACA+TGG | + | chr4.4:70582695-70582714 | None:intergenic | 45.0% | |
| ! | GAAATGGTTCTCTGAGGAGA+AGG | - | chr4.4:70582724-70582743 | MS.gene060790:intron | 45.0% |
| ! | GATTGAGGGCACATACATAG+AGG | + | chr4.4:70583477-70583496 | None:intergenic | 45.0% |
| ! | GTTTGAGGGTTTGGAGTCTT+TGG | - | chr4.4:70582895-70582914 | MS.gene060790:intron | 45.0% |
| ! | TGTGGCACTCCTCTAAGAAA+TGG | - | chr4.4:70582708-70582727 | MS.gene060790:intron | 45.0% |
| !! | TAGTTTGTTTCAAGGGGCCA+TGG | - | chr4.4:70582823-70582842 | MS.gene060790:intron | 45.0% |
| CAGCCATGTGTTTGAGGGTT+TGG | - | chr4.4:70582886-70582905 | MS.gene060790:intron | 50.0% | |
| CATGGCACTCAAATCAGCTG+TGG | - | chr4.4:70582400-70582419 | MS.gene060790:intron | 50.0% | |
| CCTTCAAAGCTTCTCGTCAG+TGG | - | chr4.4:70582626-70582645 | MS.gene060790:intron | 50.0% | |
| GGATTGCAGCCATGTGTTTG+AGG | - | chr4.4:70582880-70582899 | MS.gene060790:intron | 50.0% | |
| TGCCCTCAATCACATGTGAC+TGG | - | chr4.4:70583486-70583505 | MS.gene060790:CDS | 50.0% | |
| ! | CCACTGACGAGAAGCTTTGA+AGG | + | chr4.4:70582629-70582648 | None:intergenic | 50.0% |
| ! | CTTTGGTTGATGTGGCAGGT+GGG | - | chr4.4:70582912-70582931 | MS.gene060790:intron | 50.0% |
| ! | GAGTCTTTGGTTGATGTGGC+AGG | - | chr4.4:70582908-70582927 | MS.gene060790:intron | 50.0% |
| ! | TCTTTGGTTGATGTGGCAGG+TGG | - | chr4.4:70582911-70582930 | MS.gene060790:intron | 50.0% |
| ! | TTGCGCTTGCTAACACACGA+TGG | - | chr4.4:70582533-70582552 | MS.gene060790:CDS | 50.0% |
| !! | CACAGCTGATTTGAGTGCCA+TGG | + | chr4.4:70582402-70582421 | None:intergenic | 50.0% |
| !! | CTGACGAGAAGCTTTGAAGG+AGG | + | chr4.4:70582626-70582645 | None:intergenic | 50.0% |
| GTTGATGTGGCAGGTGGGAC+TGG | - | chr4.4:70582917-70582936 | MS.gene060790:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 70582311 | 70583960 | 70582311 | ID=MS.gene060790 |
| chr4.4 | mRNA | 70582311 | 70583960 | 70582311 | ID=MS.gene060790.t1;Parent=MS.gene060790 |
| chr4.4 | exon | 70583172 | 70583960 | 70583172 | ID=MS.gene060790.t1.exon1;Parent=MS.gene060790.t1 |
| chr4.4 | CDS | 70583172 | 70583960 | 70583172 | ID=cds.MS.gene060790.t1;Parent=MS.gene060790.t1 |
| chr4.4 | exon | 70582432 | 70582599 | 70582432 | ID=MS.gene060790.t1.exon2;Parent=MS.gene060790.t1 |
| chr4.4 | CDS | 70582432 | 70582599 | 70582432 | ID=cds.MS.gene060790.t1;Parent=MS.gene060790.t1 |
| chr4.4 | exon | 70582311 | 70582361 | 70582311 | ID=MS.gene060790.t1.exon3;Parent=MS.gene060790.t1 |
| chr4.4 | CDS | 70582311 | 70582361 | 70582311 | ID=cds.MS.gene060790.t1;Parent=MS.gene060790.t1 |
| Gene Sequence |
| Protein sequence |
