AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062799

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062799.t1 MTR_1g052715 52.249 289 115 4 2 270 13 298 4.58e-94 281
MS.gene062799.t1 MTR_1g105785 40.933 193 106 2 2 186 54 246 5.73e-49 171
MS.gene062799.t1 MTR_6g465570 63.559 118 43 0 2 119 50 167 3.39e-48 159
MS.gene062799.t1 MTR_7g110100 44.068 177 94 1 2 173 54 230 3.91e-48 168
MS.gene062799.t1 MTR_3g452170 43.503 177 95 1 2 173 54 230 4.43e-47 164
MS.gene062799.t1 MTR_7g451880 37.788 217 127 2 2 213 55 268 4.11e-45 155
MS.gene062799.t1 MTR_7g074180 37.327 217 128 2 2 213 54 267 9.38e-45 155
MS.gene062799.t1 MTR_3g015660 40.323 186 106 1 3 183 55 240 1.94e-44 151
MS.gene062799.t1 MTR_2g010270 37.611 226 124 3 2 213 35 257 2.39e-44 153
MS.gene062799.t1 MTR_4g058660 37.327 217 128 2 2 213 54 267 3.32e-44 153
MS.gene062799.t1 MTR_8g446640 36.866 217 129 2 2 213 35 248 2.56e-43 150
MS.gene062799.t1 MTR_5g030110 36.866 217 129 2 2 213 54 267 5.06e-43 150
MS.gene062799.t1 MTR_2g007410 36.866 217 129 2 2 213 54 267 5.06e-43 150
MS.gene062799.t1 MTR_7g024060 36.866 217 129 2 2 213 54 267 5.75e-43 150
MS.gene062799.t1 MTR_6g087820 36.866 217 129 2 2 213 54 267 5.75e-43 150
MS.gene062799.t1 MTR_5g081190 36.866 217 129 2 2 213 54 267 5.75e-43 150
MS.gene062799.t1 MTR_5g091480 36.866 217 129 2 2 213 54 267 5.75e-43 150
MS.gene062799.t1 MTR_5g056290 36.866 217 129 2 2 213 54 267 5.75e-43 150
MS.gene062799.t1 MTR_3g031190 36.866 217 129 2 2 213 54 267 5.75e-43 150
MS.gene062799.t1 MTR_2g005490 36.866 217 129 2 2 213 54 267 5.75e-43 150
MS.gene062799.t1 MTR_2g010570 36.866 217 129 2 2 213 54 267 5.75e-43 150
MS.gene062799.t1 MTR_1g023370 39.037 187 109 1 2 183 54 240 3.01e-42 147
MS.gene062799.t1 MTR_7g045450 39.247 186 103 1 2 187 54 229 5.05e-42 145
MS.gene062799.t1 MTR_6g073570 39.247 186 103 1 2 187 54 229 5.05e-42 145
MS.gene062799.t1 MTR_5g019960 39.572 187 106 3 2 182 60 245 4.50e-40 140
MS.gene062799.t1 MTR_5g045850 38.587 184 107 2 2 183 39 218 2.33e-38 137
MS.gene062799.t1 MTR_1g011490 34.101 217 126 3 2 213 54 258 8.71e-37 134
MS.gene062799.t1 MTR_1g082860 34.783 207 115 3 2 203 54 245 2.08e-36 131
MS.gene062799.t1 MTR_8g005940 44.697 132 73 0 2 133 54 185 1.82e-34 127
MS.gene062799.t1 MTR_7g051400 41.718 163 90 1 2 164 54 211 6.27e-34 130
MS.gene062799.t1 MTR_7g096440 32.456 228 128 2 2 203 7 234 4.31e-33 122
MS.gene062799.t1 MTR_3g007260 38.889 162 73 2 2 163 39 174 1.68e-32 118
MS.gene062799.t1 MTR_0048s0080 48.120 133 61 2 86 213 4 133 8.45e-32 121
MS.gene062799.t1 MTR_0105s0080 33.178 214 102 4 2 203 35 219 6.25e-30 114
MS.gene062799.t1 MTR_5g012180 33.880 183 90 2 2 183 39 191 4.36e-28 109
MS.gene062799.t1 MTR_3g036220 36.306 157 70 1 2 158 39 165 1.53e-26 105
MS.gene062799.t1 MTR_1g051235 41.176 119 69 1 2 119 54 172 8.14e-24 95.9
MS.gene062799.t1 MTR_2g435900 42.593 108 62 0 51 158 5 112 9.37e-24 94.0
MS.gene062799.t1 MTR_8g465040 39.831 118 71 0 2 119 54 171 2.77e-23 94.4
MS.gene062799.t1 MTR_6g024030 35.664 143 78 5 53 186 25 162 4.20e-23 95.5
MS.gene062799.t1 MTR_3g449160 40.741 108 64 0 51 158 5 112 7.45e-23 91.3
MS.gene062799.t1 MTR_7g051840 40.678 118 67 1 2 119 11 125 2.82e-22 90.1
MS.gene062799.t1 MTR_4g025750 60.606 66 26 0 6 71 2 67 1.04e-20 85.1
MS.gene062799.t1 MTR_1g492690 72.414 58 11 1 135 187 1 58 2.49e-20 90.1
MS.gene062799.t1 MTR_1g492690 53.933 89 35 1 218 300 229 317 1.02e-16 79.7
MS.gene062799.t1 MTR_5g071020 36.364 132 54 1 2 133 39 140 5.11e-20 87.0
MS.gene062799.t1 MTR_7g070110 49.367 79 40 0 2 80 38 116 1.02e-19 83.6
MS.gene062799.t1 MTR_7g039300 43.956 91 51 0 2 92 85 175 1.81e-19 85.9
MS.gene062799.t1 MTR_2g019040 33.333 129 78 2 100 223 4 129 2.28e-19 85.5
MS.gene062799.t1 MTR_4g085760 26.277 274 171 9 1 251 56 321 2.86e-18 85.1
MS.gene062799.t1 MTR_6g055250 35.632 174 91 7 18 176 27 194 3.29e-17 81.6
MS.gene062799.t1 MTR_1g043690 35.429 175 101 6 18 185 73 242 3.65e-17 82.0
MS.gene062799.t1 MTR_3g056240 32.500 160 105 3 2 160 54 211 7.35e-17 79.7
MS.gene062799.t1 MTR_3g056270 33.544 158 102 3 2 158 54 209 1.14e-16 80.5
MS.gene062799.t1 MTR_3g056270 31.875 160 106 3 2 160 213 370 1.05e-15 77.8
MS.gene062799.t1 MTR_4g036240 33.125 160 104 3 2 160 54 211 1.56e-16 80.1
MS.gene062799.t1 MTR_2g450060 32.812 192 119 7 2 185 54 243 2.71e-16 79.3
MS.gene062799.t1 MTR_4g070090 32.934 167 105 5 2 162 75 240 4.59e-16 78.6
MS.gene062799.t1 MTR_6g045290 34.722 144 90 3 18 159 324 465 6.25e-16 78.2
MS.gene062799.t1 MTR_5g004860 31.844 179 114 4 14 185 62 239 1.31e-15 77.4
MS.gene062799.t1 MTR_5g027390 32.335 167 106 5 2 162 43 208 1.77e-15 76.6
MS.gene062799.t1 MTR_5g078250 25.417 240 162 8 2 228 54 289 6.43e-15 75.1
MS.gene062799.t1 MTR_8g076570 32.919 161 98 6 3 157 52 208 3.29e-14 73.6
MS.gene062799.t1 MTR_6g052340 30.570 193 122 4 2 184 50 240 3.35e-14 73.2
MS.gene062799.t1 MTR_8g062030 27.083 240 159 7 2 228 54 290 6.54e-14 72.4
MS.gene062799.t1 MTR_7g025160 31.613 155 93 4 13 159 71 220 7.74e-14 70.5
MS.gene062799.t1 MTR_3g055660 26.842 190 128 3 1 182 78 264 1.20e-13 71.6
MS.gene062799.t1 MTR_4g014890 26.842 190 128 3 1 182 78 264 1.45e-13 71.2
MS.gene062799.t1 MTR_1g075850 26.875 160 114 3 2 160 48 205 2.65e-13 70.5
MS.gene062799.t1 MTR_6g464080 31.875 160 103 4 2 157 51 208 6.18e-13 69.3
MS.gene062799.t1 MTR_7g023280 66.667 39 13 0 2 40 13 51 8.24e-13 62.8
MS.gene062799.t1 MTR_5g053590 26.455 189 128 5 14 194 63 248 2.37e-12 67.4
MS.gene062799.t1 MTR_1g110330 37.079 89 56 0 2 90 32 120 2.72e-12 65.1
MS.gene062799.t1 MTR_8g027910 32.051 156 91 6 13 159 71 220 9.29e-12 64.3
MS.gene062799.t1 MTR_8g463820 26.776 183 113 4 2 178 54 221 9.95e-12 64.7
MS.gene062799.t1 MTR_6g039240 26.316 190 119 4 2 185 54 228 1.21e-11 64.3
MS.gene062799.t1 MTR_4g070160 30.070 143 98 2 18 159 9 150 1.25e-11 63.5
MS.gene062799.t1 MTR_6g059860 26.316 190 129 3 1 182 72 258 1.61e-11 65.1
MS.gene062799.t1 MTR_2g008650 27.950 161 111 4 2 160 56 213 2.18e-11 64.7
MS.gene062799.t1 MTR_8g479260 27.950 161 111 4 2 160 56 213 2.51e-11 64.3
MS.gene062799.t1 MTR_1g094230 26.203 187 115 5 2 181 54 224 3.44e-11 63.2
MS.gene062799.t1 MTR_2g062200 25.926 162 117 3 2 162 11 170 3.46e-11 61.6
MS.gene062799.t1 MTR_8g088810 28.415 183 123 4 2 178 54 234 3.81e-11 63.2
MS.gene062799.t1 MTR_2g054710 27.500 160 113 3 2 160 56 213 6.14e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 63 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAACTTCAGATGTTAT+TGG 0.269314 5.2:-52478359 MS.gene062799:intron
ATTTCAGGTGTCATGGGTTA+TGG 0.302395 5.2:-52477883 MS.gene062799:intron
AGAGGGTTCGACTTATGTTT+TGG 0.303687 5.2:-52478638 MS.gene062799:CDS
CATGAATCTAACCTCACTTA+TGG 0.327774 5.2:+52477376 None:intergenic
TCAGACTCTTCAAGTATGTT+AGG 0.344536 5.2:+52477841 None:intergenic
GAAGTATAAGTTGAACTTCA+TGG 0.353788 5.2:-52478566 MS.gene062799:CDS
GTATGATTCATGCAACTATC+AGG 0.367225 5.2:-52478696 MS.gene062799:CDS
CAAGGTCTTGGATGCGACTT+TGG 0.385296 5.2:-52478282 MS.gene062799:CDS
TCACAAGGAGGATCGTTTGC+TGG 0.401933 5.2:-52478458 MS.gene062799:CDS
TCTATTTGATCCTTGAAGAT+TGG 0.404730 5.2:+52478661 None:intergenic
TCAAACTTGACAAATGACTT+TGG 0.405338 5.2:+52477449 None:intergenic
TTTCCCAATGCCTTGCTAGT+TGG 0.415166 5.2:+52476711 None:intergenic
AAACCTCCTGCCAATCTTCA+AGG 0.439450 5.2:-52478671 MS.gene062799:CDS
TGCAAGCTTAAGAAGTATTA+CGG 0.443248 5.2:-52478056 MS.gene062799:intron
CTCTTATAGGAAAACGTTGA+AGG 0.447177 5.2:-52476786 MS.gene062799:intron
AAAACTTGAAAAGTCGTTCT+GGG 0.456035 5.2:+52478506 None:intergenic
TTTGATCCTTGAAGATTGGC+AGG 0.456735 5.2:+52478665 None:intergenic
TGGCAAGAGTAACAAGGTCT+TGG 0.458839 5.2:-52478294 MS.gene062799:CDS
GACTCCAACTAGCAAGGCAT+TGG 0.479496 5.2:-52476715 MS.gene062799:CDS
TGTGAGTAGCCAAGATATCC+AGG 0.482019 5.2:+52478476 None:intergenic
TTATTAAATTTCAGGTGTCA+TGG 0.482046 5.2:-52477890 MS.gene062799:intron
TGGAGCTGATTCCACTGATA+AGG 0.488659 5.2:-52476543 MS.gene062799:CDS
GCATGATTCCTCGCTTCCTA+TGG 0.493877 5.2:-52478096 MS.gene062799:CDS
TAGCAAGGCATTGGGAAAGA+TGG 0.517908 5.2:-52476706 MS.gene062799:CDS
TATTAAATTTCAGGTGTCAT+GGG 0.520803 5.2:-52477889 MS.gene062799:intron
CGTGAAAATGCAGCATTGCT+TGG 0.524535 5.2:-52478123 MS.gene062799:CDS
ACTCCAACTAGCAAGGCATT+GGG 0.530070 5.2:-52476714 MS.gene062799:CDS
CACAAGGAGGATCGTTTGCT+GGG 0.530979 5.2:-52478457 MS.gene062799:intron
TAACCTCACTTATGGCATCA+AGG 0.538434 5.2:+52477384 None:intergenic
AAGTATAAGTTGAACTTCAT+GGG 0.544635 5.2:-52478565 MS.gene062799:CDS
CTCACTTATGGCATCAAGGA+AGG 0.547124 5.2:+52477388 None:intergenic
TGTCTCGAGCTGTGGGAAGA+TGG 0.551168 5.2:-52477418 MS.gene062799:CDS
AAAGATGGTTGTTTGTGAGC+TGG 0.553610 5.2:-52476691 MS.gene062799:CDS
TCGTGTTGTGTCTCGAGCTG+TGG 0.565589 5.2:-52477426 MS.gene062799:CDS
GGATATCTTGGCTACTCACA+AGG 0.567031 5.2:-52478473 MS.gene062799:CDS
CTTCAAGGATCAAATAGAAG+AGG 0.573992 5.2:-52478656 MS.gene062799:CDS
CGTGTTGTGTCTCGAGCTGT+GGG 0.574221 5.2:-52477425 MS.gene062799:CDS
CGTCGAGACTGTTTGTGAGT+TGG 0.574832 5.2:-52476920 MS.gene062799:CDS
TATCAGTGGAATCAGCTCCA+TGG 0.576037 5.2:+52476546 None:intergenic
ATCAAAGAAACTGTGAAGGA+TGG 0.580970 5.2:-52478314 MS.gene062799:CDS
AGCTGAAATATAACAACCAT+AGG 0.592328 5.2:+52478080 None:intergenic
GCTGGCTAATCAAAATTTAG+CGG 0.595667 5.2:-52476673 MS.gene062799:intron
AGGAGTTGATTCAAGTAACA+AGG 0.598003 5.2:-52476766 MS.gene062799:CDS
TTCAGATGTTATTGGTCATG+TGG 0.599343 5.2:-52478351 MS.gene062799:intron
AGCGACATCGTCCTTATCAG+TGG 0.603044 5.2:+52476532 None:intergenic
TTCAAGGATCAAATAGAAGA+GGG 0.603682 5.2:-52478655 MS.gene062799:CDS
CTTGCTAATGATGAGGAAGA+TGG 0.613159 5.2:-52476949 MS.gene062799:CDS
TATATAGCTTGCTAATGATG+AGG 0.617218 5.2:-52476956 MS.gene062799:intron
TAGAATTCATTGTACCATGT+GGG 0.618909 5.2:-52478156 MS.gene062799:CDS
GGGTTATGGCACGAAAATGA+TGG 0.624461 5.2:-52477869 MS.gene062799:CDS
CACGGCATAATCATCCCACA+TGG 0.625776 5.2:+52478142 None:intergenic
TGTTGTGACTCCAACTAGCA+AGG 0.627219 5.2:-52476721 MS.gene062799:CDS
TATAACAACCATAGGAAGCG+AGG 0.627408 5.2:+52478088 None:intergenic
CACTATCAAAGAAACTGTGA+AGG 0.629807 5.2:-52478318 MS.gene062799:CDS
CTTCCTTGATGCCATAAGTG+AGG 0.629909 5.2:-52477387 MS.gene062799:intron
GAAGGATGGCAAGAGTAACA+AGG 0.631821 5.2:-52478300 MS.gene062799:CDS
AAGAGTCTGATTACATAGAG+AGG 0.642390 5.2:-52477828 MS.gene062799:intron
ATAGAATTCATTGTACCATG+TGG 0.644251 5.2:-52478157 MS.gene062799:CDS
GATCCTTGAAGATTGGCAGG+AGG 0.650870 5.2:+52478668 None:intergenic
TATAAGTTGAACTTCATGGG+AGG 0.684627 5.2:-52478562 MS.gene062799:CDS
TAACAAGGACGAAGTTACTG+TGG 0.689476 5.2:-52476751 MS.gene062799:CDS
GTTGGCTGATGAAAATGCTG+TGG 0.730608 5.2:-52476902 MS.gene062799:intron
TATCTTGGCTACTCACAAGG+AGG 0.740430 5.2:-52478470 MS.gene062799:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTGTTATTAAATTTC+AGG - chr5.2:52477316-52477335 MS.gene062799:intron 10.0%
!! TATAGTAAAAAAAAAGTCTA+TGG - chr5.2:52478058-52478077 MS.gene062799:CDS 15.0%
!!! CATTTTTAATTAATATAGCA+TGG + chr5.2:52477649-52477668 None:intergenic 15.0%
!!! TTTTTCCTAAAACAAAAAAA+TGG + chr5.2:52478646-52478665 None:intergenic 15.0%
!! ACATATATATCTTGCTAATA+TGG - chr5.2:52478207-52478226 MS.gene062799:intron 20.0%
!! ATAAACTGAATATCATTTCA+TGG + chr5.2:52476987-52477006 None:intergenic 20.0%
!! TGAAAGAAATGTAAATGAAT+TGG + chr5.2:52478351-52478370 None:intergenic 20.0%
!!! ACAATATGTTATATTTTGTG+TGG - chr5.2:52477925-52477944 MS.gene062799:intron 20.0%
!!! CAATATGTTATATTTTGTGT+GGG - chr5.2:52477926-52477945 MS.gene062799:intron 20.0%
!!! TTTTCCTATAAGAGAAAAAA+GGG + chr5.2:52478422-52478441 None:intergenic 20.0%
! ATTATATTTATGCGGTTTGT+AGG - chr5.2:52477030-52477049 MS.gene062799:intron 25.0%
! ATTATTAATGCCATGAAGTA+TGG - chr5.2:52477480-52477499 MS.gene062799:intron 25.0%
! ATTCATTAGACTATAATGCT+AGG + chr5.2:52477291-52477310 None:intergenic 25.0%
! TTATATTTATGCGGTTTGTA+GGG - chr5.2:52477031-52477050 MS.gene062799:intron 25.0%
!! AAGTATAAGTTGAACTTCAT+GGG - chr5.2:52476649-52476668 MS.gene062799:intron 25.0%
!! GTTTTCCTATAAGAGAAAAA+AGG + chr5.2:52478423-52478442 None:intergenic 25.0%
!! TATTAAATTTCAGGTGTCAT+GGG - chr5.2:52477325-52477344 MS.gene062799:intron 25.0%
!! TTATTAAATTTCAGGTGTCA+TGG - chr5.2:52477324-52477343 MS.gene062799:intron 25.0%
!! TTTGAAACTTCAGATGTTAT+TGG - chr5.2:52476855-52476874 MS.gene062799:intron 25.0%
!!! AAATTATGCAGCTATTTTGA+AGG + chr5.2:52477881-52477900 None:intergenic 25.0%
!!! AGTTTTATAAATTGAAGTGG+AGG - chr5.2:52477735-52477754 MS.gene062799:intron 25.0%
!!! ATGCACCATTTTTTTGTTTT+AGG - chr5.2:52478638-52478657 MS.gene062799:CDS 25.0%
!!! ATTCTGAATTTTAATCACGT+TGG - chr5.2:52477005-52477024 MS.gene062799:intron 25.0%
!!! ATTTTTTGCATAGCATTCAA+TGG - chr5.2:52476783-52476802 MS.gene062799:intron 25.0%
!!! CGTTGGTAATTATATTTATG+CGG - chr5.2:52477022-52477041 MS.gene062799:intron 25.0%
!!! GTACTATGTCTACTTTTAAT+TGG - chr5.2:52477198-52477217 MS.gene062799:intron 25.0%
!!! TCGAGTTTTATAAATTGAAG+TGG - chr5.2:52477732-52477751 MS.gene062799:intron 25.0%
!!! TCTACTTTTAATTGGTGATA+GGG - chr5.2:52477206-52477225 MS.gene062799:intron 25.0%
!!! TTATGTTTTGGAGAGATTTA+TGG - chr5.2:52476588-52476607 MS.gene062799:intron 25.0%
!!! TTGTTTTAGGAAAAAATCCA+TGG - chr5.2:52478651-52478670 MS.gene062799:CDS 25.0%
!!! TTTCAAGTTTTTTCTTTTCC+TGG - chr5.2:52476720-52476739 MS.gene062799:CDS 25.0%
AAAAACTTGAAAAGTCGTTC+TGG + chr5.2:52476712-52476731 None:intergenic 30.0%
AAAACTTGAAAAGTCGTTCT+GGG + chr5.2:52476711-52476730 None:intergenic 30.0%
AGCTGAAATATAACAACCAT+AGG + chr5.2:52477137-52477156 None:intergenic 30.0%
ATAGAATTCATTGTACCATG+TGG - chr5.2:52477057-52477076 MS.gene062799:intron 30.0%
ATGATTTACTTCAAACTCAG+AGG - chr5.2:52477579-52477598 MS.gene062799:intron 30.0%
ATTCATGAAACTCAACAGAT+AGG - chr5.2:52477854-52477873 MS.gene062799:CDS 30.0%
TAGAATTCATTGTACCATGT+GGG - chr5.2:52477058-52477077 MS.gene062799:intron 30.0%
TATATTTATGCGGTTTGTAG+GGG - chr5.2:52477032-52477051 MS.gene062799:intron 30.0%
TCAAACTTGACAAATGACTT+TGG + chr5.2:52477768-52477787 None:intergenic 30.0%
TCTATTTGATCCTTGAAGAT+TGG + chr5.2:52476556-52476575 None:intergenic 30.0%
TTCAAGGATCAAATAGAAGA+GGG - chr5.2:52476559-52476578 MS.gene062799:intron 30.0%
! GAAGTATAAGTTGAACTTCA+TGG - chr5.2:52476648-52476667 MS.gene062799:intron 30.0%
! TTTTCTTTTCCTGGATATCT+TGG - chr5.2:52476729-52476748 MS.gene062799:CDS 30.0%
!! GTCTACTTTTAATTGGTGAT+AGG - chr5.2:52477205-52477224 MS.gene062799:intron 30.0%
!! TATATAGCTTGCTAATGATG+AGG - chr5.2:52478258-52478277 MS.gene062799:intron 30.0%
!! TGCAAGCTTAAGAAGTATTA+CGG - chr5.2:52477158-52477177 MS.gene062799:intron 30.0%
!!! GAGCAATTGTTTTGAACATA+GGG + chr5.2:52476627-52476646 None:intergenic 30.0%
!!! TGAGCAATTGTTTTGAACAT+AGG + chr5.2:52476628-52476647 None:intergenic 30.0%
AAATGGTGCATTCCTTTAGA+TGG + chr5.2:52478629-52478648 None:intergenic 35.0%
AAGAGTCTGATTACATAGAG+AGG - chr5.2:52477386-52477405 MS.gene062799:intron 35.0%
ACCATATAGAATTGGTTGAC+AGG + chr5.2:52478163-52478182 None:intergenic 35.0%
AGCGGTTTATATCACTACAT+GGG - chr5.2:52478096-52478115 MS.gene062799:CDS 35.0%
ATCAAAGAAACTGTGAAGGA+TGG - chr5.2:52476900-52476919 MS.gene062799:intron 35.0%
CACTATCAAAGAAACTGTGA+AGG - chr5.2:52476896-52476915 MS.gene062799:intron 35.0%
CATGAATCTAACCTCACTTA+TGG + chr5.2:52477841-52477860 None:intergenic 35.0%
CTCTTATAGGAAAACGTTGA+AGG - chr5.2:52478428-52478447 MS.gene062799:intron 35.0%
CTTCAAGGATCAAATAGAAG+AGG - chr5.2:52476558-52476577 MS.gene062799:intron 35.0%
GACATAGTACCCAACAAATT+AGG + chr5.2:52477188-52477207 None:intergenic 35.0%
GCTGGCTAATCAAAATTTAG+CGG - chr5.2:52478541-52478560 MS.gene062799:CDS 35.0%
TAATATAGCATGGTTCACCT+CGG + chr5.2:52477639-52477658 None:intergenic 35.0%
TAGCTCAGACCATATAGAAT+TGG + chr5.2:52478171-52478190 None:intergenic 35.0%
TAGTAGTAGCAACCATCTAA+AGG - chr5.2:52478614-52478633 MS.gene062799:CDS 35.0%
TCAGACTCTTCAAGTATGTT+AGG + chr5.2:52477376-52477395 None:intergenic 35.0%
TCAGCCAATGATATTACAGT+TGG + chr5.2:52477560-52477579 None:intergenic 35.0%
TGGTCCAACTGTAATATCAT+TGG - chr5.2:52477553-52477572 MS.gene062799:intron 35.0%
TTCAGATGTTATTGGTCATG+TGG - chr5.2:52476863-52476882 MS.gene062799:intron 35.0%
! ACAAACATTTTGAGCTCAAG+AGG + chr5.2:52477972-52477991 None:intergenic 35.0%
! AGGAGTTGATTCAAGTAACA+AGG - chr5.2:52478448-52478467 MS.gene062799:intron 35.0%
! TATAAGTTGAACTTCATGGG+AGG - chr5.2:52476652-52476671 MS.gene062799:intron 35.0%
! TTACGGTAAGCCTAATTTGT+TGG - chr5.2:52477175-52477194 MS.gene062799:intron 35.0%
!! GGGAGGAACAAATGTTTTTA+AGG - chr5.2:52476669-52476688 MS.gene062799:intron 35.0%
!! TACGGTAAGCCTAATTTGTT+GGG - chr5.2:52477176-52477195 MS.gene062799:intron 35.0%
!!! TGCACCCTTTTTTCTCTTAT+AGG - chr5.2:52478415-52478434 MS.gene062799:intron 35.0%
AAAGATGGTTGTTTGTGAGC+TGG - chr5.2:52478523-52478542 MS.gene062799:CDS 40.0%
AATCAAACCGTCAATGGTCA+TGG + chr5.2:52477609-52477628 None:intergenic 40.0%
ACAAGTTGTAGTCAGATGAC+TGG - chr5.2:52477533-52477552 MS.gene062799:intron 40.0%
AGTTACGCCTATTACGATAG+AGG + chr5.2:52478037-52478056 None:intergenic 40.0%
ATGCCAACCTCTATCGTAAT+AGG - chr5.2:52478027-52478046 MS.gene062799:intron 40.0%
ATTCATCCTGCCATACTTCA+TGG + chr5.2:52477493-52477512 None:intergenic 40.0%
CTTGCTAATGATGAGGAAGA+TGG - chr5.2:52478265-52478284 MS.gene062799:intron 40.0%
GAGCGGTTTATATCACTACA+TGG - chr5.2:52478095-52478114 MS.gene062799:CDS 40.0%
GCCTGTCAACCAATTCTATA+TGG - chr5.2:52478159-52478178 MS.gene062799:CDS 40.0%
TAACAAGGACGAAGTTACTG+TGG - chr5.2:52478463-52478482 MS.gene062799:CDS 40.0%
TAACCTCACTTATGGCATCA+AGG + chr5.2:52477833-52477852 None:intergenic 40.0%
TATAACAACCATAGGAAGCG+AGG + chr5.2:52477129-52477148 None:intergenic 40.0%
TGGATGACAGTATCATTGAG+CGG - chr5.2:52478078-52478097 MS.gene062799:CDS 40.0%
TTAATGCCATGAAGTATGGC+AGG - chr5.2:52477484-52477503 MS.gene062799:intron 40.0%
TTTGATCCTTGAAGATTGGC+AGG + chr5.2:52476552-52476571 None:intergenic 40.0%
! AGAGGGTTCGACTTATGTTT+TGG - chr5.2:52476576-52476595 MS.gene062799:intron 40.0%
! ATTTCAGGTGTCATGGGTTA+TGG - chr5.2:52477331-52477350 MS.gene062799:intron 40.0%
! CAAGCAATGCTGCATTTTCA+CGG + chr5.2:52477093-52477112 None:intergenic 40.0%
AAACCTCCTGCCAATCTTCA+AGG - chr5.2:52476543-52476562 MS.gene062799:CDS 45.0%
ACGCCTATTACGATAGAGGT+TGG + chr5.2:52478033-52478052 None:intergenic 45.0%
AGGATGACCATGACCATTGA+CGG - chr5.2:52477599-52477618 MS.gene062799:intron 45.0%
CGACTCAATCAAACCGTCAA+TGG + chr5.2:52477615-52477634 None:intergenic 45.0%
CGTGAAAATGCAGCATTGCT+TGG - chr5.2:52477091-52477110 MS.gene062799:intron 45.0%
CTCACTTATGGCATCAAGGA+AGG + chr5.2:52477829-52477848 None:intergenic 45.0%
CTTCCTTGATGCCATAAGTG+AGG - chr5.2:52477827-52477846 MS.gene062799:intron 45.0%
GAAGGATGGCAAGAGTAACA+AGG - chr5.2:52476914-52476933 MS.gene062799:CDS 45.0%
GGATATCTTGGCTACTCACA+AGG - chr5.2:52476741-52476760 MS.gene062799:CDS 45.0%
GGGTTATGGCACGAAAATGA+TGG - chr5.2:52477345-52477364 MS.gene062799:intron 45.0%
GTTGGCTGATGAAAATGCTG+TGG - chr5.2:52478312-52478331 MS.gene062799:CDS 45.0%
TATCAGTGGAATCAGCTCCA+TGG + chr5.2:52478671-52478690 None:intergenic 45.0%
TATCTTGGCTACTCACAAGG+AGG - chr5.2:52476744-52476763 MS.gene062799:CDS 45.0%
TGGCAAGAGTAACAAGGTCT+TGG - chr5.2:52476920-52476939 MS.gene062799:CDS 45.0%
TGTGAGTAGCCAAGATATCC+AGG + chr5.2:52476741-52476760 None:intergenic 45.0%
TGTTGTGACTCCAACTAGCA+AGG - chr5.2:52478493-52478512 MS.gene062799:CDS 45.0%
TTTCCCAATGCCTTGCTAGT+TGG + chr5.2:52478506-52478525 None:intergenic 45.0%
! ACTCCAACTAGCAAGGCATT+GGG - chr5.2:52478500-52478519 MS.gene062799:CDS 45.0%
! TGGAGCTGATTCCACTGATA+AGG - chr5.2:52478671-52478690 MS.gene062799:CDS 45.0%
!! TAGCAAGGCATTGGGAAAGA+TGG - chr5.2:52478508-52478527 MS.gene062799:CDS 45.0%
AGCGACATCGTCCTTATCAG+TGG + chr5.2:52478685-52478704 None:intergenic 50.0%
CAAGGTCTTGGATGCGACTT+TGG - chr5.2:52476932-52476951 MS.gene062799:CDS 50.0%
CACAAGGAGGATCGTTTGCT+GGG - chr5.2:52476757-52476776 MS.gene062799:CDS 50.0%
CACGGCATAATCATCCCACA+TGG + chr5.2:52477075-52477094 None:intergenic 50.0%
CGTCGAGACTGTTTGTGAGT+TGG - chr5.2:52478294-52478313 MS.gene062799:CDS 50.0%
GACTCCAACTAGCAAGGCAT+TGG - chr5.2:52478499-52478518 MS.gene062799:CDS 50.0%
GATCCTTGAAGATTGGCAGG+AGG + chr5.2:52476549-52476568 None:intergenic 50.0%
GCATGATTCCTCGCTTCCTA+TGG - chr5.2:52477118-52477137 MS.gene062799:intron 50.0%
TCACAAGGAGGATCGTTTGC+TGG - chr5.2:52476756-52476775 MS.gene062799:CDS 50.0%
CGGTTTGATTGAGTCGACCG+AGG - chr5.2:52477619-52477638 MS.gene062799:intron 55.0%
CGTGTTGTGTCTCGAGCTGT+GGG - chr5.2:52477789-52477808 MS.gene062799:intron 55.0%
TCGTGTTGTGTCTCGAGCTG+TGG - chr5.2:52477788-52477807 MS.gene062799:intron 55.0%
TGTCTCGAGCTGTGGGAAGA+TGG - chr5.2:52477796-52477815 MS.gene062799:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 52476520 52478716 52476520 ID=MS.gene062799
chr5.2 mRNA 52476520 52478716 52476520 ID=MS.gene062799.t1;Parent=MS.gene062799
chr5.2 exon 52478458 52478716 52478458 ID=MS.gene062799.t1.exon1;Parent=MS.gene062799.t1
chr5.2 CDS 52478458 52478716 52478458 ID=cds.MS.gene062799.t1;Parent=MS.gene062799.t1
chr5.2 exon 52478272 52478368 52478272 ID=MS.gene062799.t1.exon2;Parent=MS.gene062799.t1
chr5.2 CDS 52478272 52478368 52478272 ID=cds.MS.gene062799.t1;Parent=MS.gene062799.t1
chr5.2 exon 52478057 52478184 52478057 ID=MS.gene062799.t1.exon3;Parent=MS.gene062799.t1
chr5.2 CDS 52478057 52478184 52478057 ID=cds.MS.gene062799.t1;Parent=MS.gene062799.t1
chr5.2 exon 52477829 52477898 52477829 ID=MS.gene062799.t1.exon4;Parent=MS.gene062799.t1
chr5.2 CDS 52477829 52477898 52477829 ID=cds.MS.gene062799.t1;Parent=MS.gene062799.t1
chr5.2 exon 52477388 52477484 52477388 ID=MS.gene062799.t1.exon5;Parent=MS.gene062799.t1
chr5.2 CDS 52477388 52477484 52477388 ID=cds.MS.gene062799.t1;Parent=MS.gene062799.t1
chr5.2 exon 52476903 52476971 52476903 ID=MS.gene062799.t1.exon6;Parent=MS.gene062799.t1
chr5.2 CDS 52476903 52476971 52476903 ID=cds.MS.gene062799.t1;Parent=MS.gene062799.t1
chr5.2 exon 52476674 52476799 52476674 ID=MS.gene062799.t1.exon7;Parent=MS.gene062799.t1
chr5.2 CDS 52476674 52476799 52476674 ID=cds.MS.gene062799.t1;Parent=MS.gene062799.t1
chr5.2 exon 52476520 52476576 52476520 ID=MS.gene062799.t1.exon8;Parent=MS.gene062799.t1
chr5.2 CDS 52476520 52476576 52476520 ID=cds.MS.gene062799.t1;Parent=MS.gene062799.t1
Gene Sequence

>MS.gene062799

ATGATTCATGCAACTATCAGGAAAAACCTCCTGCCAATCTTCAAGGATCAAATAGAAGAGGGTTCGACTTATGTTTTGGAGAGATTTATGGTTGCTCAGAATGACCCTATGTTCAAAACAATTGCTCAGAAGTATAAGTTGAACTTCATGGGAGGAACAAATGTTTTTAAGGTCAATGCTACTGAAATCCCAGAACGACTTTTCAAGTTTTTTCTTTTCCTGGATATCTTGGCTACTCACAAGGAGGATCGTTTGCTGGGTAAATTTTTTGCATAGCATTCAATGGTTTTAACTTTGATGCACTTTTAGAAAACTCATATTTATCTTATAAATATTTTGAAACTTCAGATGTTATTGGTCATGTGGTTGAGAAAGACACTATCAAAGAAACTGTGAAGGATGGCAAGAGTAACAAGGTCTTGGATGCGACTTTGGAAGATTTAGAGTAAGTTGACTGAAAGTGACCATGAAATGATATTCAGTTTATTCTGAATTTTAATCACGTTGGTAATTATATTTATGCGGTTTGTAGGGGAAATAGAATTCATTGTACCATGTGGGATGATTATGCCGTGAAAATGCAGCATTGCTTGGATACGCATGATTCCTCGCTTCCTATGGTTGTTATATTTCAGCTATGCAAGCTTAAGAAGTATTACGGTAAGCCTAATTTGTTGGGTACTATGTCTACTTTTAATTGGTGATAGGGTTAGATAATCATTTTTAATGAGTATAAAGCTTTAAGAAAGATAATGTTGTGAAGTAATACCTAGCATTATAGTCTAATGAATTCTAGAAAATTTGTTATTAAATTTCAGGTGTCATGGGTTATGGCACGAAAATGATGGTGAATCCTAACATACTTGAAGAGTCTGATTACATAGAGAGGTGTGACACATGTGTTTTAAATGTGTAGTTAGTCTCGATATTTTCACTGTTATAAGATGAAGTATTGATTAGATTATTAATGCCATGAAGTATGGCAGGATGAATGATGCAAATGTTGAGCTCACACAAGTTGTAGTCAGATGACTGGTCCAACTGTAATATCATTGGCTGATGATTTACTTCAAACTCAGAGGATGACCATGACCATTGACGGTTTGATTGAGTCGACCGAGGTGAACCATGCTATATTAATTAAAAATGAATTTAAATATGAATTATCTGATTATTGAGATTGAATCTAAATATGAATTATCTGATTATTAATCGAGTTTTATAAATTGAAGTGGAGGTTTGAATCCAAAGTCATTTGTCAAGTTTGATCGTGTTGTGTCTCGAGCTGTGGGAAGATGGTCACAATCCTTCCTTGATGCCATAAGTGAGGTTAGATTCATGAAACTCAACAGATAGGACCTTCAAAATAGCTGCATAATTTTTCTCAAACTATACTTTTTACTTGACAATATGTTATATTTTGTGTGGGCAAACACTTCTACTGAATGTCCTCTTGAGCTCAAAATGTTTGTTGACAAGCGAATGTTGTTCAAAGTTGAAGTGAGCGATGCCAACCTCTATCGTAATAGGCGTAACTATATAGTAAAAAAAAAGTCTATGGATGACAGTATCATTGAGCGGTTTATATCACTACATGGGATAAGTGTAAAGTTCAACTAACATTATTTTCTTTTGAACTGCCTGTCAACCAATTCTATATGGTCTGAGCTATATTTTGTTCTTACTTACATATATATCTTGCTAATATGGTCTGAGCTATATTTTTTTCTTACTTACATATATAGCTTGCTAATGATGAGGAAGATGGTGCATACGTCGAGACTGTTTGTGAGTTGGCTGATGAAAATGCTGTGGTATATTTTCATTTCCAATTCATTTACATTTCTTTCATGTATAAATGATTTTCTTATATATGAATCAACAAACTAATAAAGTGCACCCTTTTTTCTCTTATAGGAAAACGTTGAAGGAGTTGATTCAAGTAACAAGGACGAAGTTACTGTGGTTGATTCTGTTGTGACTCCAACTAGCAAGGCATTGGGAAAGATGGTTGTTTGTGAGCTGGCTAATCAAAATTTAGCGGTATATATTTATATCAAATTAGATCAAATTATTTTGATTTGTTAACGAATTTAGTAGTAGCAACCATCTAAAGGAATGCACCATTTTTTTGTTTTAGGAAAAAATCCATGGAGCTGATTCCACTGATAAGGACGATGTCGCTATAGTTGATTAA
Protein sequence

>MS.gene062799.t1

MIHATIRKNLLPIFKDQIEEGSTYVLERFMVAQNDPMFKTIAQKYKLNFMGGTNVFKVNATEIPERLFKFFLFLDILATHKEDRLLDVIGHVVEKDTIKETVKDGKSNKVLDATLEDLEGNRIHCTMWDDYAVKMQHCLDTHDSSLPMVVIFQLCKLKKYYGVMGYGTKMMVNPNILEESDYIESGGLNPKSFVKFDRVVSRAVGRWSQSFLDAISELANDEEDGAYVETVCELADENAVENVEGVDSSNKDEVTVVDSVVTPTSKALGKMVVCELANQNLAEKIHGADSTDKDDVAIVD