AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051181


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051181.t1 MTR_1g492690 40.244 246 48 6 10 166 55 290 1.31e-38 135
MS.gene051181.t1 MTR_1g052715 41.216 148 35 2 1 148 203 298 1.59e-15 73.2
MS.gene051181.t1 MTR_0048s0080 41.509 106 59 2 43 148 203 305 2.58e-15 72.8
MS.gene051181.t1 MTR_2g040680 30.729 192 83 8 1 162 61 232 7.55e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAATCTAACCTCACTTA+TGG 0.327774 2.3:-48524569 None:intergenic
TTTCCCAATGCCTTGCTAGT+TGG 0.415166 2.3:-48525233 None:intergenic
CTCTTATAGGAAAACGTTGA+AGG 0.447177 2.3:+48525158 MS.gene051181:intron
GACTCCAACTAGCAAGGCAT+TGG 0.479496 2.3:+48525229 MS.gene051181:CDS
TGGAGCTGATTCCACTGATA+AGG 0.488659 2.3:+48525401 MS.gene051181:CDS
TAGCAAGGCATTGGGAAAGA+TGG 0.517908 2.3:+48525238 MS.gene051181:CDS
AATCAACCCGTCAATGATCA+TGG 0.522602 2.3:-48524337 None:intergenic
TGGTCCAACTGTAGTATCAT+TGG 0.525087 2.3:+48524284 MS.gene051181:CDS
ACTCCAACTAGCAAGGCATT+GGG 0.530070 2.3:+48525230 MS.gene051181:CDS
TAACCTCACTTATGGCATCA+AGG 0.538434 2.3:-48524561 None:intergenic
ATGAAGTATGGCAGGATGAA+TGG 0.541959 2.3:+48524222 None:intergenic
CTCACTTATGGCATCAAGGA+AGG 0.547124 2.3:-48524557 None:intergenic
TGTCTCGAGCTGTGGGAAGA+TGG 0.551168 2.3:+48524527 MS.gene051181:CDS
AAAGATGGTTGTTTGTGAGC+TGG 0.553610 2.3:+48525253 MS.gene051181:CDS
TCAGCCAATGATACTACAGT+TGG 0.557688 2.3:-48524288 None:intergenic
AGGATGACCATGATCATTGA+CGG 0.560152 2.3:+48524330 MS.gene051181:CDS
TCGTGTTGTGTCTCGAGCTG+TGG 0.565589 2.3:+48524519 MS.gene051181:CDS
CGTGTTGTGTCTCGAGCTGT+GGG 0.574221 2.3:+48524520 MS.gene051181:CDS
CGTCGAGACTGTTTGTGAGT+TGG 0.574832 2.3:+48525024 MS.gene051181:CDS
TATCAGTGGAATCAGCTCCA+TGG 0.576037 2.3:-48525398 None:intergenic
GGATGACCATGATCATTGAC+GGG 0.582784 2.3:+48524331 MS.gene051181:CDS
AGGAGTTGATTCAAGTAACA+AGG 0.598003 2.3:+48525178 MS.gene051181:CDS
AACGACATCGTCCTTATCAG+TGG 0.600855 2.3:-48525412 None:intergenic
CTTGCTAATGATGAGGAAGA+TGG 0.613159 2.3:+48524995 MS.gene051181:CDS
TATATAGCTTGCTAATGATG+AGG 0.617218 2.3:+48524988 MS.gene051181:intron
ATGATTTACTTCAAACTCAG+AGG 0.622816 2.3:+48524310 MS.gene051181:CDS
CAAGCTGTTAGTCAGATGAC+TGG 0.625318 2.3:+48524264 MS.gene051181:CDS
TGTTGTGACTCCAACTAGCA+AGG 0.627219 2.3:+48525223 MS.gene051181:CDS
CTTCCTTGATGCCATAAGTG+AGG 0.629909 2.3:+48524558 MS.gene051181:CDS
TAACAAGGACAAAGTTACTG+TGG 0.681233 2.3:+48525193 MS.gene051181:CDS
CGGGTTGATTGAGTCGACTG+AGG 0.691957 2.3:+48524350 MS.gene051181:CDS
GCTGGCTAATCAAAATTCAG+CGG 0.718491 2.3:+48525271 MS.gene051181:CDS
GTTGGCTGATGAAAATGCTG+TGG 0.730608 2.3:+48525042 MS.gene051181:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAGTAAAAAAAAGTCTA+TGG + chr2.3:48524788-48524807 MS.gene051181:intron 15.0%
!!! CATTTTTAATTAATATAGCA+TGG - chr2.3:48524380-48524399 None:intergenic 15.0%
!!! TTTTTCCTAAAACAAAAAAA+TGG - chr2.3:48525376-48525395 None:intergenic 15.0%
!! TGAAAGAAATGTAAATGAAT+TGG - chr2.3:48525081-48525100 None:intergenic 20.0%
!!! CAATATGTTATATTTTGTGT+AGG + chr2.3:48524657-48524676 MS.gene051181:intron 20.0%
!!! TTTTCCTATAAGAGAAAAAA+GGG - chr2.3:48525152-48525171 None:intergenic 20.0%
!! GTTTTCCTATAAGAGAAAAA+AGG - chr2.3:48525153-48525172 None:intergenic 25.0%
!!! AAATTATGCAGCTATTTTGA+AGG - chr2.3:48524612-48524631 None:intergenic 25.0%
!!! ACATATATAGCTTGCTAATA+TGG + chr2.3:48524937-48524956 MS.gene051181:intron 25.0%
!!! AGTTTTATAAATTGAAGTGG+AGG + chr2.3:48524466-48524485 MS.gene051181:intron 25.0%
!!! ATGCACCATTTTTTTGTTTT+AGG + chr2.3:48525368-48525387 MS.gene051181:intron 25.0%
!!! TCGAGTTTTATAAATTGAAG+TGG + chr2.3:48524463-48524482 MS.gene051181:intron 25.0%
!!! TTGTTTTAGGAAAAAATCCA+TGG + chr2.3:48525381-48525400 MS.gene051181:intron 25.0%
ATGATTTACTTCAAACTCAG+AGG + chr2.3:48524310-48524329 MS.gene051181:CDS 30.0%
ATTCATGAAACTCAACAGAT+AGG + chr2.3:48524585-48524604 MS.gene051181:intron 30.0%
TCAAACTTGACAAATGACTT+TGG - chr2.3:48524499-48524518 None:intergenic 30.0%
!! TATATAGCTTGCTAATGATG+AGG + chr2.3:48524988-48525007 MS.gene051181:intron 30.0%
AAATGGTGCATTCCTTTAGA+TGG - chr2.3:48525359-48525378 None:intergenic 35.0%
ACCATATAGAATTGGTTGAC+AGG - chr2.3:48524893-48524912 None:intergenic 35.0%
AGCGGTTTATATCACTACAT+GGG + chr2.3:48524826-48524845 MS.gene051181:intron 35.0%
CATGAATCTAACCTCACTTA+TGG - chr2.3:48524572-48524591 None:intergenic 35.0%
CTCTTATAGGAAAACGTTGA+AGG + chr2.3:48525158-48525177 MS.gene051181:intron 35.0%
TAACAAGGACAAAGTTACTG+TGG + chr2.3:48525193-48525212 MS.gene051181:CDS 35.0%
TAGCTCAGACCATATAGAAT+TGG - chr2.3:48524901-48524920 None:intergenic 35.0%
TAGTAGTAGCAACCATCTAA+AGG + chr2.3:48525344-48525363 MS.gene051181:intron 35.0%
! ACAAACATTTTGAGCTCAAG+AGG - chr2.3:48524703-48524722 None:intergenic 35.0%
! AGGAGTTGATTCAAGTAACA+AGG + chr2.3:48525178-48525197 MS.gene051181:CDS 35.0%
!!! TGCACCCTTTTTTCTCTTAT+AGG + chr2.3:48525145-48525164 MS.gene051181:intron 35.0%
AAAGATGGTTGTTTGTGAGC+TGG + chr2.3:48525253-48525272 MS.gene051181:CDS 40.0%
AATCAACCCGTCAATGATCA+TGG - chr2.3:48524340-48524359 None:intergenic 40.0%
AGGATGACCATGATCATTGA+CGG + chr2.3:48524330-48524349 MS.gene051181:CDS 40.0%
ATGCCAACCTCTATCGTAAT+AGG + chr2.3:48524758-48524777 MS.gene051181:intron 40.0%
CTTGCTAATGATGAGGAAGA+TGG + chr2.3:48524995-48525014 MS.gene051181:CDS 40.0%
GAGCGGTTTATATCACTACA+TGG + chr2.3:48524825-48524844 MS.gene051181:intron 40.0%
GCCTGTCAACCAATTCTATA+TGG + chr2.3:48524889-48524908 MS.gene051181:intron 40.0%
GCTGGCTAATCAAAATTCAG+CGG + chr2.3:48525271-48525290 MS.gene051181:CDS 40.0%
TAACCTCACTTATGGCATCA+AGG - chr2.3:48524564-48524583 None:intergenic 40.0%
TCAGCCAATGATACTACAGT+TGG - chr2.3:48524291-48524310 None:intergenic 40.0%
TGGATGACAGTATCATTGAG+CGG + chr2.3:48524808-48524827 MS.gene051181:intron 40.0%
TGGTCCAACTGTAGTATCAT+TGG + chr2.3:48524284-48524303 MS.gene051181:CDS 40.0%
AACGACATCGTCCTTATCAG+TGG - chr2.3:48525415-48525434 None:intergenic 45.0%
AGTTGCGCCTATTACGATAG+AGG - chr2.3:48524768-48524787 None:intergenic 45.0%
CAAGCTGTTAGTCAGATGAC+TGG + chr2.3:48524264-48524283 MS.gene051181:CDS 45.0%
CTCACTTATGGCATCAAGGA+AGG - chr2.3:48524560-48524579 None:intergenic 45.0%
CTTCCTTGATGCCATAAGTG+AGG + chr2.3:48524558-48524577 MS.gene051181:CDS 45.0%
GGATGACCATGATCATTGAC+GGG + chr2.3:48524331-48524350 MS.gene051181:CDS 45.0%
GTTGGCTGATGAAAATGCTG+TGG + chr2.3:48525042-48525061 MS.gene051181:CDS 45.0%
TATCAGTGGAATCAGCTCCA+TGG - chr2.3:48525401-48525420 None:intergenic 45.0%
TGTTGTGACTCCAACTAGCA+AGG + chr2.3:48525223-48525242 MS.gene051181:CDS 45.0%
TTTCCCAATGCCTTGCTAGT+TGG - chr2.3:48525236-48525255 None:intergenic 45.0%
! ACTCCAACTAGCAAGGCATT+GGG + chr2.3:48525230-48525249 MS.gene051181:CDS 45.0%
! TGGAGCTGATTCCACTGATA+AGG + chr2.3:48525401-48525420 MS.gene051181:CDS 45.0%
!! TAGCAAGGCATTGGGAAAGA+TGG + chr2.3:48525238-48525257 MS.gene051181:CDS 45.0%
CGTCGAGACTGTTTGTGAGT+TGG + chr2.3:48525024-48525043 MS.gene051181:CDS 50.0%
GACTCCAACTAGCAAGGCAT+TGG + chr2.3:48525229-48525248 MS.gene051181:CDS 50.0%
GCGCCTATTACGATAGAGGT+TGG - chr2.3:48524764-48524783 None:intergenic 50.0%
CGTGTTGTGTCTCGAGCTGT+GGG + chr2.3:48524520-48524539 MS.gene051181:CDS 55.0%
TCGTGTTGTGTCTCGAGCTG+TGG + chr2.3:48524519-48524538 MS.gene051181:CDS 55.0%
TGTCTCGAGCTGTGGGAAGA+TGG + chr2.3:48524527-48524546 MS.gene051181:CDS 55.0%
!! CGGGTTGATTGAGTCGACTG+AGG + chr2.3:48524350-48524369 MS.gene051181:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 48524237 48525446 48524237 ID=MS.gene051181
chr2.3 mRNA 48524237 48525446 48524237 ID=MS.gene051181.t1;Parent=MS.gene051181
chr2.3 exon 48524237 48524371 48524237 ID=MS.gene051181.t1.exon1;Parent=MS.gene051181.t1
chr2.3 CDS 48524237 48524371 48524237 ID=cds.MS.gene051181.t1;Parent=MS.gene051181.t1
chr2.3 exon 48524502 48524579 48524502 ID=MS.gene051181.t1.exon2;Parent=MS.gene051181.t1
chr2.3 CDS 48524502 48524579 48524502 ID=cds.MS.gene051181.t1;Parent=MS.gene051181.t1
chr2.3 exon 48524679 48524750 48524679 ID=MS.gene051181.t1.exon3;Parent=MS.gene051181.t1
chr2.3 CDS 48524679 48524750 48524679 ID=cds.MS.gene051181.t1;Parent=MS.gene051181.t1
chr2.3 exon 48524995 48525063 48524995 ID=MS.gene051181.t1.exon4;Parent=MS.gene051181.t1
chr2.3 CDS 48524995 48525063 48524995 ID=cds.MS.gene051181.t1;Parent=MS.gene051181.t1
chr2.3 exon 48525167 48525292 48525167 ID=MS.gene051181.t1.exon5;Parent=MS.gene051181.t1
chr2.3 CDS 48525167 48525292 48525167 ID=cds.MS.gene051181.t1;Parent=MS.gene051181.t1
chr2.3 exon 48525390 48525446 48525390 ID=MS.gene051181.t1.exon6;Parent=MS.gene051181.t1
chr2.3 CDS 48525390 48525446 48525390 ID=cds.MS.gene051181.t1;Parent=MS.gene051181.t1
Gene Sequence

>MS.gene051181

ATGAATGGTGCAAATGTTGAGCTCACACAAGCTGTTAGTCAGATGACTGGTCCAACTGTAGTATCATTGGCTGATGATTTACTTCAAACTCAGAGGATGACCATGATCATTGACGGGTTGATTGAGTCGACTGAGGTGAACCATGCTATATTAATTAAAAATGAATTTAAATATGAATTATCTGATTATTGAGATTGAATCTAAATATGAATTATCTGATTATTAATCGAGTTTTATAAATTGAAGTGGAGGTTTGAATCCAAAGTCATTTGTCAAGTTTGATCGTGTTGTGTCTCGAGCTGTGGGAAGATGGTCACAATCCTTCCTTGATGCCATAAGTGAGGTTAGATTCATGAAACTCAACAGATAGGACCTTCAAAATAGCTGCATAATTTTTCTCAAACTATACTTTTTACTTGACAATATGTTATATTTTGTGTAGGCAAACACTTCTGCTGAATGTCCTCTTGAGCTCAAAATGTTTGTTGACAAGCGAATGTTGTTCAAAGTTGAAGTGAGCGATGCCAACCTCTATCGTAATAGGCGCAACTATATAGTAAAAAAAAGTCTATGGATGACAGTATCATTGAGCGGTTTATATCACTACATGGGATAAGTGTAAAGTTCAACTAACATTATTTTCTTTTGAACTGCCTGTCAACCAATTCTATATGGTCTGAGCTATATTTTGTTCTTACTTACATATATAGCTTGCTAATATGGTCTGAGCTATATTTTTTTCTTACTTACATATATAGCTTGCTAATGATGAGGAAGATGGTGCATACGTCGAGACTGTTTGTGAGTTGGCTGATGAAAATGCTGTGGTATATTTTCATTTCCAATTCATTTACATTTCTTTCATGTATAAATGATTTTCTTATATATGAATCAACAAACTAATAAAGTGCACCCTTTTTTCTCTTATAGGAAAACGTTGAAGGAGTTGATTCAAGTAACAAGGACAAAGTTACTGTGGTTGATTCTGTTGTGACTCCAACTAGCAAGGCATTGGGAAAGATGGTTGTTTGTGAGCTGGCTAATCAAAATTCAGCGGTATATATTTGTATCAAATTAGATCAAATTATTTTGATTTGCTAACGAATTTAGTAGTAGCAACCATCTAAAGGAATGCACCATTTTTTTGTTTTAGGAAAAAATCCATGGAGCTGATTCCACTGATAAGGACGATGTCGTTATAGTTGATTAA

Protein sequence

>MS.gene051181.t1

MNGANVELTQAVSQMTGPTVVSLADDLLQTQRMTMIIDGLIESTESFVKFDRVVSRAVGRWSQSFLDAISEANTSAECPLELKMFVDKRMLFKVELANDEEDGAYVETVCELADENAVENVEGVDSSNKDKVTVVDSVVTPTSKALGKMVVCELANQNSAEKIHGADSTDKDDVVIVD