Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062890.t1 | XP_003606627.1 | 97.3 | 148 | 4 | 0 | 1 | 148 | 1 | 148 | 3.80E-45 | 191 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062890.t1 | O22582 | 92.8 | 111 | 8 | 0 | 38 | 148 | 37 | 147 | 3.0e-46 | 186.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062890.t1 | G7JTQ7 | 97.3 | 148 | 4 | 0 | 1 | 148 | 1 | 148 | 2.8e-45 | 191.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04972 | MS.gene062890 | 0.81583 | 7.56E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062890.t1 | MTR_4g063220 | 99.324 | 148 | 1 | 0 | 1 | 148 | 1 | 148 | 3.84e-102 | 289 |
| MS.gene062890.t1 | MTR_4g063990 | 99.324 | 148 | 1 | 0 | 1 | 148 | 1 | 148 | 3.84e-102 | 289 |
| MS.gene062890.t1 | MTR_4g063200 | 99.324 | 148 | 1 | 0 | 1 | 148 | 1 | 148 | 3.84e-102 | 289 |
| MS.gene062890.t1 | MTR_4g064020 | 99.324 | 148 | 1 | 0 | 1 | 148 | 1 | 148 | 3.84e-102 | 289 |
| MS.gene062890.t1 | MTR_4g071180 | 99.324 | 148 | 1 | 0 | 1 | 148 | 1 | 148 | 8.19e-102 | 288 |
| MS.gene062890.t1 | MTR_4g070240 | 99.324 | 148 | 1 | 0 | 1 | 148 | 1 | 148 | 8.19e-102 | 288 |
| MS.gene062890.t1 | MTR_4g070245 | 97.973 | 148 | 3 | 0 | 1 | 148 | 1 | 148 | 4.03e-100 | 284 |
| MS.gene062890.t1 | MTR_4g063240 | 98.649 | 148 | 1 | 1 | 1 | 148 | 1 | 147 | 1.11e-99 | 283 |
| MS.gene062890.t1 | MTR_4g063275 | 96.622 | 148 | 5 | 0 | 1 | 148 | 1 | 148 | 8.15e-99 | 280 |
| MS.gene062890.t1 | MTR_4g064983 | 98.611 | 144 | 2 | 0 | 1 | 144 | 1 | 144 | 7.90e-97 | 279 |
| MS.gene062890.t1 | MTR_4g088025 | 93.919 | 148 | 4 | 1 | 1 | 148 | 1 | 143 | 7.97e-89 | 255 |
| MS.gene062890.t1 | MTR_1g090780 | 88.514 | 148 | 6 | 3 | 1 | 148 | 1 | 137 | 1.62e-78 | 229 |
| MS.gene062890.t1 | MTR_1g492910 | 87.838 | 148 | 7 | 3 | 1 | 148 | 1 | 137 | 8.01e-78 | 227 |
| MS.gene062890.t1 | MTR_1g068600 | 92.793 | 111 | 4 | 1 | 38 | 148 | 30 | 136 | 3.27e-64 | 192 |
| MS.gene062890.t1 | MTR_7g099960 | 92.793 | 111 | 5 | 1 | 38 | 148 | 31 | 138 | 6.03e-64 | 192 |
| MS.gene062890.t1 | MTR_2g084480 | 61.798 | 89 | 34 | 0 | 59 | 147 | 136 | 224 | 4.66e-37 | 127 |
| MS.gene062890.t1 | MTR_8g069990 | 68.817 | 93 | 21 | 3 | 15 | 107 | 4 | 88 | 3.43e-34 | 115 |
| MS.gene062890.t1 | MTR_4g063270 | 90.196 | 51 | 5 | 0 | 86 | 136 | 1 | 51 | 5.07e-27 | 95.9 |
| MS.gene062890.t1 | MTR_7g114580 | 90.244 | 41 | 4 | 0 | 108 | 148 | 11 | 51 | 9.18e-20 | 77.4 |
| MS.gene062890.t1 | MTR_4g032777 | 36.792 | 106 | 63 | 2 | 31 | 132 | 5 | 110 | 1.69e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062890.t1 | AT3G45980 | 88.742 | 151 | 13 | 2 | 1 | 148 | 1 | 150 | 1.41e-83 | 242 |
| MS.gene062890.t1 | AT2G28720 | 86.093 | 151 | 18 | 1 | 1 | 148 | 1 | 151 | 9.31e-81 | 235 |
| MS.gene062890.t1 | AT1G07790 | 86.928 | 153 | 10 | 3 | 1 | 148 | 1 | 148 | 7.39e-80 | 233 |
| MS.gene062890.t1 | AT5G59910 | 91.176 | 136 | 9 | 2 | 13 | 148 | 18 | 150 | 2.55e-77 | 226 |
| MS.gene062890.t1 | AT3G46030 | 94.444 | 126 | 6 | 1 | 23 | 148 | 21 | 145 | 8.32e-76 | 222 |
| MS.gene062890.t1 | AT2G37470 | 82.432 | 148 | 15 | 3 | 1 | 147 | 1 | 138 | 5.77e-71 | 210 |
| MS.gene062890.t1 | AT3G53650 | 81.208 | 149 | 16 | 3 | 1 | 148 | 1 | 138 | 4.43e-69 | 205 |
| MS.gene062890.t1 | AT5G22880 | 87.586 | 145 | 16 | 2 | 4 | 148 | 3 | 145 | 4.09e-67 | 200 |
| MS.gene062890.t1 | AT5G02570 | 79.730 | 148 | 14 | 1 | 1 | 148 | 1 | 132 | 9.33e-67 | 199 |
| MS.gene062890.t1 | AT3G09480 | 84.127 | 126 | 16 | 1 | 23 | 148 | 5 | 126 | 2.05e-62 | 187 |
| MS.gene062890.t1 | AT1G08170 | 61.176 | 85 | 33 | 0 | 61 | 145 | 151 | 235 | 2.87e-37 | 127 |
Find 44 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGGCTTCTTCTCTCCTTT+TGG | 0.266976 | 4.1:-31908120 | None:intergenic |
| GGTAGCTTCTTTCCAGCCTT+TGG | 0.299167 | 4.1:-31908207 | None:intergenic |
| GCTTGTTCTGCCCGGTGAAT+TGG | 0.328727 | 4.1:+31908482 | MS.gene062890:CDS |
| TTGCTTGAGATACCAATGTC+TGG | 0.335174 | 4.1:-31908333 | None:intergenic |
| TCTGCGGGTTTCTTCTCTGC+TGG | 0.399254 | 4.1:-31908138 | None:intergenic |
| GCCTTTGGCTTCTTCTCCGC+CGG | 0.402654 | 4.1:-31908192 | None:intergenic |
| GCGGCTGAACCACCTTCCTT+TGG | 0.410543 | 4.1:-31908228 | None:intergenic |
| GTGGACTTCTTCTCCTCTGC+GGG | 0.437960 | 4.1:-31908153 | None:intergenic |
| TCTCTTCTTCTTCTTGTCTC+CGG | 0.444808 | 4.1:-31908250 | None:intergenic |
| TGCTGTTTCTGAGGGTACTA+AGG | 0.448307 | 4.1:+31908512 | MS.gene062890:CDS |
| ATACAAGATCTACATCTTCA+AGG | 0.451479 | 4.1:+31908293 | MS.gene062890:CDS |
| CATCTTCAAGGTTCTGAAGC+AGG | 0.454934 | 4.1:+31908305 | MS.gene062890:CDS |
| GGGAAATTCAGACCGCGGTC+AGG | 0.473209 | 4.1:+31908460 | MS.gene062890:CDS |
| ATCCTTTCAATGGCGCCAAA+AGG | 0.496211 | 4.1:+31908105 | None:intergenic |
| GCGGAGAAGAAGCCAAAGGC+TGG | 0.504484 | 4.1:+31908195 | MS.gene062890:CDS |
| AAGGAAGGTGGTTCAGCCGC+CGG | 0.504946 | 4.1:+31908231 | MS.gene062890:CDS |
| CGCCGGAGCTTTCTCTGCGA+CGG | 0.521178 | 4.1:-31908175 | None:intergenic |
| GGCTAAGCATGCTGTTTCTG+AGG | 0.531832 | 4.1:+31908503 | MS.gene062890:CDS |
| TGCTTGAGATACCAATGTCT+GGG | 0.532971 | 4.1:-31908332 | None:intergenic |
| TGGTATCTCAAGCAAAGCCA+TGG | 0.533917 | 4.1:+31908341 | MS.gene062890:CDS |
| CATCTTCGAGAAGCTTGCTC+AGG | 0.534069 | 4.1:+31908389 | MS.gene062890:CDS |
| AGCATGCTTAGCCAATTCAC+CGG | 0.550997 | 4.1:-31908493 | None:intergenic |
| AGAAGCCGACGATCACTTCA+AGG | 0.553571 | 4.1:+31908439 | MS.gene062890:CDS |
| GCTAAGCATGCTGTTTCTGA+GGG | 0.554009 | 4.1:+31908504 | MS.gene062890:CDS |
| GCGGTCAGGCTTGTTCTGCC+CGG | 0.555879 | 4.1:+31908474 | MS.gene062890:CDS |
| ATTTCCCTTGAAGTGATCGT+CGG | 0.564140 | 4.1:-31908444 | None:intergenic |
| CTTCTTCTTCTTGTCTCCGG+CGG | 0.574610 | 4.1:-31908247 | None:intergenic |
| CACCGTCGCAGAGAAAGCTC+CGG | 0.578048 | 4.1:+31908173 | MS.gene062890:CDS |
| GCATGCTTAGCCAATTCACC+GGG | 0.596462 | 4.1:-31908492 | None:intergenic |
| AGGAATCTTCAAGACTCGCT+AGG | 0.600474 | 4.1:+31908409 | MS.gene062890:CDS |
| AAGCAGGTTCACCCAGACAT+TGG | 0.601452 | 4.1:+31908321 | MS.gene062890:CDS |
| CGGAGCTTTCTCTGCGACGG+TGG | 0.603516 | 4.1:-31908172 | None:intergenic |
| GGAAAGAAGCTACCAAAGGA+AGG | 0.624050 | 4.1:+31908216 | MS.gene062890:CDS |
| GAAACTGTTCATGATTCCCA+TGG | 0.633384 | 4.1:-31908358 | None:intergenic |
| GGCTGGAAAGAAGCTACCAA+AGG | 0.634160 | 4.1:+31908212 | MS.gene062890:CDS |
| GAAGCCGACGATCACTTCAA+GGG | 0.638033 | 4.1:+31908440 | MS.gene062890:CDS |
| CGTCGCAGAGAAAGCTCCGG+CGG | 0.643171 | 4.1:+31908176 | MS.gene062890:CDS |
| GGTATCTCAAGCAAAGCCAT+GGG | 0.654333 | 4.1:+31908342 | MS.gene062890:CDS |
| AAGAAGCTACCAAAGGAAGG+TGG | 0.666846 | 4.1:+31908219 | MS.gene062890:CDS |
| GGTGGACTTCTTCTCCTCTG+CGG | 0.668086 | 4.1:-31908154 | None:intergenic |
| TCCGGCGGAGAAGAAGCCAA+AGG | 0.682003 | 4.1:+31908191 | MS.gene062890:CDS |
| TTCAAGGGAAATTCAGACCG+CGG | 0.690059 | 4.1:+31908455 | MS.gene062890:CDS |
| GGGCAGAACAAGCCTGACCG+CGG | 0.695125 | 4.1:-31908472 | None:intergenic |
| AGCAGAGAAGAAACCCGCAG+AGG | 0.698236 | 4.1:+31908140 | MS.gene062890:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATACAAGATCTACATCTTCA+AGG | + | chr4.1:31908293-31908312 | MS.gene062890:CDS | 30.0% | |
| ATTTCCCTTGAAGTGATCGT+CGG | - | chr4.1:31908447-31908466 | None:intergenic | 40.0% | |
| GAAACTGTTCATGATTCCCA+TGG | - | chr4.1:31908361-31908380 | None:intergenic | 40.0% | |
| TCTCTTCTTCTTCTTGTCTC+CGG | - | chr4.1:31908253-31908272 | None:intergenic | 40.0% | |
| TGCTTGAGATACCAATGTCT+GGG | - | chr4.1:31908335-31908354 | None:intergenic | 40.0% | |
| TTGCTTGAGATACCAATGTC+TGG | - | chr4.1:31908336-31908355 | None:intergenic | 40.0% | |
| AAGAAGCTACCAAAGGAAGG+TGG | + | chr4.1:31908219-31908238 | MS.gene062890:CDS | 45.0% | |
| AGCATGCTTAGCCAATTCAC+CGG | - | chr4.1:31908496-31908515 | None:intergenic | 45.0% | |
| AGGAATCTTCAAGACTCGCT+AGG | + | chr4.1:31908409-31908428 | MS.gene062890:CDS | 45.0% | |
| GGAAAGAAGCTACCAAAGGA+AGG | + | chr4.1:31908216-31908235 | MS.gene062890:CDS | 45.0% | |
| GGTATCTCAAGCAAAGCCAT+GGG | + | chr4.1:31908342-31908361 | MS.gene062890:CDS | 45.0% | |
| TGGTATCTCAAGCAAAGCCA+TGG | + | chr4.1:31908341-31908360 | MS.gene062890:CDS | 45.0% | |
| TTCAAGGGAAATTCAGACCG+CGG | + | chr4.1:31908455-31908474 | MS.gene062890:CDS | 45.0% | |
| !! | CATCTTCAAGGTTCTGAAGC+AGG | + | chr4.1:31908305-31908324 | MS.gene062890:CDS | 45.0% |
| !! | GCTAAGCATGCTGTTTCTGA+GGG | + | chr4.1:31908504-31908523 | MS.gene062890:CDS | 45.0% |
| !! | TGCTGTTTCTGAGGGTACTA+AGG | + | chr4.1:31908512-31908531 | MS.gene062890:CDS | 45.0% |
| AAGCAGGTTCACCCAGACAT+TGG | + | chr4.1:31908321-31908340 | MS.gene062890:CDS | 50.0% | |
| AGAAGCCGACGATCACTTCA+AGG | + | chr4.1:31908439-31908458 | MS.gene062890:CDS | 50.0% | |
| CTTCTTCTTCTTGTCTCCGG+CGG | - | chr4.1:31908250-31908269 | None:intergenic | 50.0% | |
| GAAGCCGACGATCACTTCAA+GGG | + | chr4.1:31908440-31908459 | MS.gene062890:CDS | 50.0% | |
| GCATGCTTAGCCAATTCACC+GGG | - | chr4.1:31908495-31908514 | None:intergenic | 50.0% | |
| GGCTGGAAAGAAGCTACCAA+AGG | + | chr4.1:31908212-31908231 | MS.gene062890:CDS | 50.0% | |
| GGTAGCTTCTTTCCAGCCTT+TGG | - | chr4.1:31908210-31908229 | None:intergenic | 50.0% | |
| ! | CATCTTCGAGAAGCTTGCTC+AGG | + | chr4.1:31908389-31908408 | MS.gene062890:CDS | 50.0% |
| !! | GCTGGCTTCTTCTCTCCTTT+TGG | - | chr4.1:31908123-31908142 | None:intergenic | 50.0% |
| !! | GGCTAAGCATGCTGTTTCTG+AGG | + | chr4.1:31908503-31908522 | MS.gene062890:CDS | 50.0% |
| AGCAGAGAAGAAACCCGCAG+AGG | + | chr4.1:31908140-31908159 | MS.gene062890:CDS | 55.0% | |
| GCTTGTTCTGCCCGGTGAAT+TGG | + | chr4.1:31908482-31908501 | MS.gene062890:CDS | 55.0% | |
| GTGGACTTCTTCTCCTCTGC+GGG | - | chr4.1:31908156-31908175 | None:intergenic | 55.0% | |
| TCTGCGGGTTTCTTCTCTGC+TGG | - | chr4.1:31908141-31908160 | None:intergenic | 55.0% | |
| ! | GGTGGACTTCTTCTCCTCTG+CGG | - | chr4.1:31908157-31908176 | None:intergenic | 55.0% |
| AAGGAAGGTGGTTCAGCCGC+CGG | + | chr4.1:31908231-31908250 | MS.gene062890:CDS | 60.0% | |
| CACCGTCGCAGAGAAAGCTC+CGG | + | chr4.1:31908173-31908192 | MS.gene062890:CDS | 60.0% | |
| GCGGAGAAGAAGCCAAAGGC+TGG | + | chr4.1:31908195-31908214 | MS.gene062890:CDS | 60.0% | |
| GCGGCTGAACCACCTTCCTT+TGG | - | chr4.1:31908231-31908250 | None:intergenic | 60.0% | |
| GGGAAATTCAGACCGCGGTC+AGG | + | chr4.1:31908460-31908479 | MS.gene062890:CDS | 60.0% | |
| TCCGGCGGAGAAGAAGCCAA+AGG | + | chr4.1:31908191-31908210 | MS.gene062890:CDS | 60.0% | |
| ! | GCCTTTGGCTTCTTCTCCGC+CGG | - | chr4.1:31908195-31908214 | None:intergenic | 60.0% |
| CGCCGGAGCTTTCTCTGCGA+CGG | - | chr4.1:31908178-31908197 | None:intergenic | 65.0% | |
| CGGAGCTTTCTCTGCGACGG+TGG | - | chr4.1:31908175-31908194 | None:intergenic | 65.0% | |
| CGTCGCAGAGAAAGCTCCGG+CGG | + | chr4.1:31908176-31908195 | MS.gene062890:CDS | 65.0% | |
| GGGCAGAACAAGCCTGACCG+CGG | - | chr4.1:31908475-31908494 | None:intergenic | 65.0% | |
| ! | GCGGTCAGGCTTGTTCTGCC+CGG | + | chr4.1:31908474-31908493 | MS.gene062890:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 31908114 | 31908560 | 31908114 | ID=MS.gene062890 |
| chr4.1 | mRNA | 31908114 | 31908560 | 31908114 | ID=MS.gene062890.t1;Parent=MS.gene062890 |
| chr4.1 | exon | 31908114 | 31908560 | 31908114 | ID=MS.gene062890.t1.exon1;Parent=MS.gene062890.t1 |
| chr4.1 | CDS | 31908114 | 31908560 | 31908114 | ID=cds.MS.gene062890.t1;Parent=MS.gene062890.t1 |
| Gene Sequence |
| Protein sequence |
