AlfalfaGEDB | MS.gene04972

M. sativa cultivar XinJiangDaYe / MS.gene04972

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04972.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04972.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04972.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049596	MS.gene04972	0.8237	1.20E-53	-1.69E-46
MS.gene049705	MS.gene04972	0.808088	3.68E-50	-1.69E-46
MS.gene04972	MS.gene050297	0.826173	3.14E-54	-1.69E-46
MS.gene04972	MS.gene05124	0.812433	4.25E-51	-1.69E-46
MS.gene04972	MS.gene051263	0.817731	2.83E-52	-1.69E-46
MS.gene04972	MS.gene054643	0.827677	1.37E-54	-1.69E-46
MS.gene04972	MS.gene055957	0.81259	3.93E-51	-1.69E-46
MS.gene04972	MS.gene058818	0.838112	3.49E-57	-1.69E-46
MS.gene04972	MS.gene058844	0.825772	3.91E-54	-1.69E-46
MS.gene04972	MS.gene062890	0.81583	7.56E-52	-1.69E-46
MS.gene04972	MS.gene071185	0.81634	5.82E-52	-1.69E-46
MS.gene04972	MS.gene08109	0.828855	7.13E-55	-1.69E-46
MS.gene04972	MS.gene09234	0.806178	9.33E-50	-1.69E-46
MS.gene04972	MS.gene24699	0.830337	3.11E-55	-1.69E-46
MS.gene04972	MS.gene27823	0.837861	4.05E-57	-1.69E-46
MS.gene04972	MS.gene32703	0.816556	5.20E-52	-1.69E-46
MS.gene04972	MS.gene32915	0.800724	1.26E-48	-1.69E-46
MS.gene04972	MS.gene38714	0.822194	2.70E-53	-1.69E-46
MS.gene04972	MS.gene38942	0.802017	6.85E-49	-1.69E-46
MS.gene04972	MS.gene42665	0.807474	4.97E-50	-1.69E-46
MS.gene04972	MS.gene50734	0.827859	1.24E-54	-1.69E-46
MS.gene04972	MS.gene52703	0.807255	5.53E-50	-1.69E-46
MS.gene04972	MS.gene52975	0.803503	3.38E-49	-1.69E-46
MS.gene04972	MS.gene64883	0.808063	3.72E-50	-1.69E-46
MS.gene04972	MS.gene66158	0.800728	1.26E-48	-1.69E-46
MS.gene04972	MS.gene66159	0.814578	1.43E-51	-1.69E-46
MS.gene04972	MS.gene66433	0.811821	5.78E-51	-1.69E-46
MS.gene04972	MS.gene68101	0.804327	2.28E-49	-1.69E-46
MS.gene04972	MS.gene68103	0.809904	1.50E-50	-1.69E-46
MS.gene04972	MS.gene70896	0.806766	7.01E-50	-1.69E-46
MS.gene04972	MS.gene70898	0.817478	3.23E-52	-1.69E-46
MS.gene04972	MS.gene78972	0.8029	4.50E-49	-1.69E-46
MS.gene04972	MS.gene80180	0.806808	6.87E-50	-1.69E-46
MS.gene04972	MS.gene80183	0.810983	8.79E-51	-1.69E-46
MS.gene04972	MS.gene90876	0.812638	3.83E-51	-1.69E-46
MS.gene04972	MS.gene93102	0.814728	1.33E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene04392	MS.gene04972	PPI
MS.gene04068	MS.gene04972	PPI
MS.gene04434	MS.gene04972	PPI
MS.gene04972	MS.gene049705	PPI
MS.gene08121	MS.gene04972	PPI
MS.gene022932	MS.gene04972	PPI
MS.gene04972	MS.gene68545	PPI
MS.gene88681	MS.gene04972	PPI
MS.gene070244	MS.gene04972	PPI
MS.gene035896	MS.gene04972	PPI
MS.gene73136	MS.gene04972	PPI
MS.gene64920	MS.gene04972	PPI
MS.gene008276	MS.gene04972	PPI
MS.gene070907	MS.gene04972	PPI
MS.gene60093	MS.gene04972	PPI
MS.gene004124	MS.gene04972	PPI
MS.gene009027	MS.gene04972	PPI
MS.gene018578	MS.gene04972	PPI
MS.gene04972	MS.gene60093	PPI
MS.gene31196	MS.gene04972	PPI
MS.gene010219	MS.gene04972	PPI
MS.gene04052	MS.gene04972	PPI
MS.gene08122	MS.gene04972	PPI
MS.gene060465	MS.gene04972	PPI
MS.gene05623	MS.gene04972	PPI
MS.gene043967	MS.gene04972	PPI
MS.gene061867	MS.gene04972	PPI
MS.gene84536	MS.gene04972	PPI
MS.gene028629	MS.gene04972	PPI
MS.gene04972	MS.gene018578	PPI
MS.gene71215	MS.gene04972	PPI
MS.gene04972	MS.gene018580	PPI
MS.gene04972	MS.gene004124	PPI
MS.gene061866	MS.gene04972	PPI
MS.gene061870	MS.gene04972	PPI
MS.gene004121	MS.gene04972	PPI
MS.gene035718	MS.gene04972	PPI
MS.gene66225	MS.gene04972	PPI
MS.gene071136	MS.gene04972	PPI
MS.gene04972	MS.gene64650	PPI
MS.gene012390	MS.gene04972	PPI
MS.gene09873	MS.gene04972	PPI
MS.gene04972	MS.gene061874	PPI
MS.gene78578	MS.gene04972	PPI
MS.gene79195	MS.gene04972	PPI
MS.gene035721	MS.gene04972	PPI
MS.gene048705	MS.gene04972	PPI
MS.gene064557	MS.gene04972	PPI
MS.gene038469	MS.gene04972	PPI
MS.gene04972	MS.gene004121	PPI
MS.gene82546	MS.gene04972	PPI
MS.gene61017	MS.gene04972	PPI
MS.gene04433	MS.gene04972	PPI
MS.gene04452	MS.gene04972	PPI
MS.gene64650	MS.gene04972	PPI
MS.gene035787	MS.gene04972	PPI
MS.gene04067	MS.gene04972	PPI
MS.gene061874	MS.gene04972	PPI
MS.gene89487	MS.gene04972	PPI
MS.gene035719	MS.gene04972	PPI
MS.gene58854	MS.gene04972	PPI
MS.gene016624	MS.gene04972	PPI
MS.gene04972	MS.gene89488	PPI
MS.gene70955	MS.gene04972	PPI
MS.gene040383	MS.gene04972	PPI
MS.gene070242	MS.gene04972	PPI
MS.gene072493	MS.gene04972	PPI
MS.gene035720	MS.gene04972	PPI
MS.gene30920	MS.gene04972	PPI
MS.gene048746	MS.gene04972	PPI
MS.gene010220	MS.gene04972	PPI
MS.gene08053	MS.gene04972	PPI
MS.gene061872	MS.gene04972	PPI
MS.gene88680	MS.gene04972	PPI
MS.gene68545	MS.gene04972	PPI
MS.gene070241	MS.gene04972	PPI
MS.gene056215	MS.gene04972	PPI
MS.gene061869	MS.gene04972	PPI
MS.gene049821	MS.gene04972	PPI
MS.gene022946	MS.gene04972	PPI
MS.gene62520	MS.gene04972	PPI
MS.gene018580	MS.gene04972	PPI
MS.gene04064	MS.gene04972	PPI
MS.gene061876	MS.gene04972	PPI
MS.gene05619	MS.gene04972	PPI
MS.gene05608	MS.gene04972	PPI
MS.gene012391	MS.gene04972	PPI
MS.gene049705	MS.gene04972	PPI
MS.gene09871	MS.gene04972	PPI
MS.gene035897	MS.gene04972	PPI
MS.gene04972	MS.gene016624	PPI
MS.gene04576	MS.gene04972	PPI
MS.gene060463	MS.gene04972	PPI
MS.gene89488	MS.gene04972	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04972.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene04972.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene04972.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04972.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene04972.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 36 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGCCAATCGACGAATTGC+AGG	0.267346	3.3:+58985901	None:intergenic
GGAAGAACCCTCTATGGATT+TGG	0.370047	3.3:-58985715	MS.gene04972:CDS
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3.3:-58985721	MS.gene04972:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.3:-58985830	MS.gene04972:CDS
GGGAGGTAAGGGACTTGGAA+AGG	0.449159	3.3:-58985980	MS.gene04972:CDS
GGAGGTAAGGGACTTGGAAA+GGG	0.467307	3.3:-58985979	MS.gene04972:CDS
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	3.3:-58985985	MS.gene04972:CDS
TTCAACCTCCAAATCCATAG+AGG	0.476286	3.3:+58985707	None:intergenic
CTGTTGAAAATGTCAGGTCG+TGG	0.487154	3.3:-58986006	None:intergenic
CAATCGACGAATTGCAGGCT+TGG	0.491738	3.3:+58985906	None:intergenic
AGGTCGTGGAAAGGGAGGTA+AGG	0.492819	3.3:-58985992	MS.gene04972:CDS
AGTTCTCCGCGATAACATCC+AGG	0.504812	3.3:-58985935	MS.gene04972:CDS
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.3:-58985712	MS.gene04972:CDS
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.3:+58985834	None:intergenic
CAAGCCTGCAATTCGTCGAT+TGG	0.511374	3.3:-58985905	MS.gene04972:CDS
TTACATACACTGAGCATGCT+AGG	0.520723	3.3:-58985786	MS.gene04972:CDS
GAGGAAGACGGTGACAGCCA+TGG	0.546349	3.3:-58985764	MS.gene04972:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.547891	3.3:-58985960	MS.gene04972:CDS
AAAATGTCAGGTCGTGGAAA+GGG	0.548959	3.3:-58986000	MS.gene04972:CDS
GGTAAGGGACTTGGAAAGGG+AGG	0.560743	3.3:-58985976	MS.gene04972:CDS
GGTCGTGGAAAGGGAGGTAA+GGG	0.564245	3.3:-58985991	MS.gene04972:CDS
GTCTATGCTTTGAAGAGACA+AGG	0.570048	3.3:-58985736	MS.gene04972:CDS
GAAAATGTCAGGTCGTGGAA+AGG	0.584382	3.3:-58986001	None:intergenic
CGTCGATTGGCGAGAAGAGG+CGG	0.593362	3.3:-58985892	MS.gene04972:CDS
GGCGGTGTTAAGAGAATCAG+TGG	0.595979	3.3:-58985874	MS.gene04972:CDS
ATTCGTCGATTGGCGAGAAG+AGG	0.604517	3.3:-58985895	MS.gene04972:CDS
TTGCAGGCTTGGTGATACCC+TGG	0.613296	3.3:+58985917	None:intergenic
CAAAGCATAGACAACATCCA+TGG	0.629842	3.3:+58985747	None:intergenic
TCAACCTCCAAATCCATAGA+GGG	0.633476	3.3:+58985708	None:intergenic
ATGTCAGGTCGTGGAAAGGG+AGG	0.643397	3.3:-58985997	MS.gene04972:CDS
TGATACCCTGGATGTTATCG+CGG	0.665173	3.3:+58985929	None:intergenic
TGAGCATGCTAGGAGGAAGA+CGG	0.671956	3.3:-58985776	MS.gene04972:CDS
GTTCTCCGCGATAACATCCA+GGG	0.672360	3.3:-58985934	MS.gene04972:CDS
TTGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.3:-58985966	MS.gene04972:CDS
TTGATCTATGAAGAAACCAG+AGG	0.704889	3.3:-58985850	MS.gene04972:CDS
CATACACTGAGCATGCTAGG+AGG	0.707859	3.3:-58985783	MS.gene04972:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	-	chr3.3:58985855-58985874	MS.gene04972:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3.3:58985875-58985894	MS.gene04972:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.3:58985961-58985980	None:intergenic	40.0%
	TCAACCTCCAAATCCATAGA+GGG	+	chr3.3:58986000-58986019	None:intergenic	40.0%
	TTACATACACTGAGCATGCT+AGG	-	chr3.3:58985919-58985938	MS.gene04972:CDS	40.0%
!!	GTCTATGCTTTGAAGAGACA+AGG	-	chr3.3:58985969-58985988	MS.gene04972:CDS	40.0%
	AGAACCCTCTATGGATTTGG+AGG	-	chr3.3:58985993-58986012	MS.gene04972:CDS	45.0%
	AGACAAGGAAGAACCCTCTA+TGG	-	chr3.3:58985984-58986003	MS.gene04972:CDS	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3.3:58985874-58985893	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.3:58985990-58986009	MS.gene04972:CDS	45.0%
	TGATACCCTGGATGTTATCG+CGG	+	chr3.3:58985779-58985798	None:intergenic	45.0%
	AGTTCTCCGCGATAACATCC+AGG	-	chr3.3:58985770-58985789	MS.gene04972:CDS	50.0%
	CAAGCCTGCAATTCGTCGAT+TGG	-	chr3.3:58985800-58985819	MS.gene04972:CDS	50.0%
	CAATCGACGAATTGCAGGCT+TGG	+	chr3.3:58985802-58985821	None:intergenic	50.0%
	CATACACTGAGCATGCTAGG+AGG	-	chr3.3:58985922-58985941	MS.gene04972:CDS	50.0%
	GGAGGTAAGGGACTTGGAAA+GGG	-	chr3.3:58985726-58985745	MS.gene04972:CDS	50.0%
	GTTCTCCGCGATAACATCCA+GGG	-	chr3.3:58985771-58985790	MS.gene04972:CDS	50.0%
	TGAGCATGCTAGGAGGAAGA+CGG	-	chr3.3:58985929-58985948	MS.gene04972:CDS	50.0%
!	ATTCGTCGATTGGCGAGAAG+AGG	-	chr3.3:58985810-58985829	MS.gene04972:CDS	50.0%
!	GGCGGTGTTAAGAGAATCAG+TGG	-	chr3.3:58985831-58985850	MS.gene04972:CDS	50.0%
!	TTGGAAAGGGAGGTGCAAAG+AGG	-	chr3.3:58985739-58985758	MS.gene04972:CDS	50.0%
	AGGTCGTGGAAAGGGAGGTA+AGG	-	chr3.3:58985713-58985732	MS.gene04972:CDS	55.0%
	ATGTCAGGTCGTGGAAAGGG+AGG	-	chr3.3:58985708-58985727	MS.gene04972:CDS	55.0%
	CTCGCCAATCGACGAATTGC+AGG	+	chr3.3:58985807-58985826	None:intergenic	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr3.3:58985720-58985739	MS.gene04972:CDS	55.0%
	GGGAGGTAAGGGACTTGGAA+AGG	-	chr3.3:58985725-58985744	MS.gene04972:CDS	55.0%
	GGTAAGGGACTTGGAAAGGG+AGG	-	chr3.3:58985729-58985748	MS.gene04972:CDS	55.0%
	GGTCGTGGAAAGGGAGGTAA+GGG	-	chr3.3:58985714-58985733	MS.gene04972:CDS	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	-	chr3.3:58985745-58985764	MS.gene04972:CDS	55.0%
!!	TTGCAGGCTTGGTGATACCC+TGG	+	chr3.3:58985791-58985810	None:intergenic	55.0%
	GAGGAAGACGGTGACAGCCA+TGG	-	chr3.3:58985941-58985960	MS.gene04972:CDS	60.0%
!	CGTCGATTGGCGAGAAGAGG+CGG	-	chr3.3:58985813-58985832	MS.gene04972:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	58985708	58986019	58985708	ID=MS.gene04972
chr3.3	mRNA	58985708	58986019	58985708	ID=MS.gene04972.t1;Parent=MS.gene04972
chr3.3	exon	58985708	58986019	58985708	ID=MS.gene04972.t1.exon1;Parent=MS.gene04972.t1
chr3.3	CDS	58985708	58986019	58985708	ID=cds.MS.gene04972.t1;Parent=MS.gene04972.t1

Gene Sequence	>MS.gene04972 ATGTCAGGTCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGGCATAGGAAAGTTCTCCGCGATAACATCCAGGGTATCACCAAGCCTGCAATTCGTCGATTGGCGAGAAGAGGCGGTGTTAAGAGAATCAGTGGATTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAACGTCATTCGTGATGCAGTTACATACACTGAGCATGCTAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAAGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene04972.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG