AlfalfaGEDB | MS.gene063016

KEGG: K09422
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063016.t1	XP_003607200.1	97.8	91	2	0	5	95	195	285	4.20E-45	190.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063016.t1	P81393	56.5	62	21	2	10	65	171	232	1.2e-11	70.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063016.t1	G7JNF6	97.8	91	2	0	5	95	195	285	3.0e-45	190.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051720	MS.gene063016	0.800215	1.60E-48	-1.69E-46
MS.gene052584	MS.gene063016	0.802876	4.56E-49	-1.69E-46
MS.gene054991	MS.gene063016	0.827151	1.83E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063016.t1	MTR_4g073420	97.895	95	2	0	1	95	191	285	1.64e-65	199

Find 21 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCACCTGCTTATGATTTCT+TGG	0.191888	4.2:-35081632	MS.gene063016:CDS
TGGGTTTGAAAGGTGGTGTT+TGG	0.257123	4.2:-35081612	MS.gene063016:CDS
ATGAACACAAACATGGTTAA+AGG	0.278944	4.2:-35081850	MS.gene063016:CDS
GGGTTTGAAAGGTGGTGTTT+GGG	0.359186	4.2:-35081611	MS.gene063016:CDS
GCACCTGCTTATGATTTCTT+GGG	0.393603	4.2:-35081631	MS.gene063016:CDS
GGAAATTCTAGCAGTGGAAA+TGG	0.412132	4.2:-35081661	MS.gene063016:CDS
GTTAGCTCAAGATTCAAATC+AGG	0.414650	4.2:+35081808	None:intergenic
TTTGGGTTTGCAGAATAGTA+AGG	0.428430	4.2:-35081707	MS.gene063016:CDS
TGAACACAAACATGGTTAAA+GGG	0.446158	4.2:-35081849	MS.gene063016:CDS
CACAAACATGGTTAAAGGGT+TGG	0.452473	4.2:-35081845	MS.gene063016:CDS
TATGATTTCTTGGGTTTGAA+AGG	0.475287	4.2:-35081622	MS.gene063016:CDS
ATTTCCAACAATTTCATCAC+AGG	0.514829	4.2:+35081678	None:intergenic
TGTACCTGTGATGAAATTGT+TGG	0.517658	4.2:-35081682	MS.gene063016:CDS
GATTTCTTGGGTTTGAAAGG+TGG	0.519673	4.2:-35081619	MS.gene063016:CDS
ATTGTTGGAAATTCTAGCAG+TGG	0.566489	4.2:-35081667	MS.gene063016:CDS
TCGTGTTCTTGAACAAGTGG+TGG	0.576080	4.2:+35081778	None:intergenic
AAACCCAAGAAATCATAAGC+AGG	0.597600	4.2:+35081628	None:intergenic
TCATCGTGTTCTTGAACAAG+TGG	0.617174	4.2:+35081775	None:intergenic
GGTGGTGTTTGGGATTACAA+AGG	0.622062	4.2:-35081601	MS.gene063016:CDS
AACAATTCAGAAACAGAGAG+AGG	0.655779	4.2:-35081753	MS.gene063016:CDS
TCAAGAAATGAACACAAACA+TGG	0.660042	4.2:-35081857	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTGTTTTGTTTGTAGTTT+GGG	-	chr4.2:35081711-35081730	MS.gene063016:CDS	25.0%
!!!	TTTCTGTTTTGTTTGTAGTT+TGG	-	chr4.2:35081710-35081729	MS.gene063016:CDS	25.0%
	ATGAACACAAACATGGTTAA+AGG	-	chr4.2:35081585-35081604	MS.gene063016:CDS	30.0%
	ATTTCCAACAATTTCATCAC+AGG	+	chr4.2:35081760-35081779	None:intergenic	30.0%
	TATGATTTCTTGGGTTTGAA+AGG	-	chr4.2:35081813-35081832	MS.gene063016:CDS	30.0%
	TGAACACAAACATGGTTAAA+GGG	-	chr4.2:35081586-35081605	MS.gene063016:CDS	30.0%
	AAACCCAAGAAATCATAAGC+AGG	+	chr4.2:35081810-35081829	None:intergenic	35.0%
	AACAATTCAGAAACAGAGAG+AGG	-	chr4.2:35081682-35081701	MS.gene063016:CDS	35.0%
	ATTGTTGGAAATTCTAGCAG+TGG	-	chr4.2:35081768-35081787	MS.gene063016:CDS	35.0%
	GTTAGCTCAAGATTCAAATC+AGG	+	chr4.2:35081630-35081649	None:intergenic	35.0%
	TGTACCTGTGATGAAATTGT+TGG	-	chr4.2:35081753-35081772	MS.gene063016:CDS	35.0%
!	TTTGGGTTTGCAGAATAGTA+AGG	-	chr4.2:35081728-35081747	MS.gene063016:CDS	35.0%
	CACAAACATGGTTAAAGGGT+TGG	-	chr4.2:35081590-35081609	MS.gene063016:CDS	40.0%
	GATTTCTTGGGTTTGAAAGG+TGG	-	chr4.2:35081816-35081835	MS.gene063016:CDS	40.0%
	GCACCTGCTTATGATTTCTT+GGG	-	chr4.2:35081804-35081823	MS.gene063016:CDS	40.0%
	GGAAATTCTAGCAGTGGAAA+TGG	-	chr4.2:35081774-35081793	MS.gene063016:CDS	40.0%
	TCATCGTGTTCTTGAACAAG+TGG	+	chr4.2:35081663-35081682	None:intergenic	40.0%
	TGCACCTGCTTATGATTTCT+TGG	-	chr4.2:35081803-35081822	MS.gene063016:CDS	40.0%
	GGGTTTGAAAGGTGGTGTTT+GGG	-	chr4.2:35081824-35081843	MS.gene063016:CDS	45.0%
	TCGTGTTCTTGAACAAGTGG+TGG	+	chr4.2:35081660-35081679	None:intergenic	45.0%
	TGGGTTTGAAAGGTGGTGTT+TGG	-	chr4.2:35081823-35081842	MS.gene063016:CDS	45.0%
!	GGTGGTGTTTGGGATTACAA+AGG	-	chr4.2:35081834-35081853	MS.gene063016:CDS	45.0%

Gene Sequence	>MS.gene063016 ATGAACACAAACATGGTTAAAGGGTTGGTTTTAGAACGTTGTCCTGATTTGAATCTTGAGCTAACAATTAGTCCACCACTTGTTCAAGAACACGATGAACAATTCAGAAACAGAGAGAGGAACAATTTCTGTTTTGTTTGTAGTTTGGGTTTGCAGAATAGTAAGGATTGTACCTGTGATGAAATTGTTGGAAATTCTAGCAGTGGAAATGGTTCTACTGCACCTGCTTATGATTTCTTGGGTTTGAAAGGTGGTGTTTGGGATTACAAAGGCTTAGAAATGAAATGA
Protein sequence	>MS.gene063016.t1 MNTNMVKGLVLERCPDLNLELTISPPLVQEHDEQFRNRERNNFCFVCSLGLQNSKDCTCDEIVGNSSSGNGSTAPAYDFLGLKGGVWDYKGLEMK

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	35081585	35081872	35081585	ID=MS.gene063016
chr4.2	mRNA	35081585	35081872	35081585	ID=MS.gene063016.t1;Parent=MS.gene063016
chr4.2	exon	35081585	35081872	35081585	ID=MS.gene063016.t1.exon1;Parent=MS.gene063016.t1
chr4.2	CDS	35081585	35081872	35081585	ID=cds.MS.gene063016.t1;Parent=MS.gene063016.t1

M. sativa cultivar XinJiangDaYe / MS.gene063016