AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054991


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054991.t1 MTR_7g066880 81.677 322 26 2 5 296 41 359 0.0 522
MS.gene054991.t1 MTR_7g066890 52.308 325 121 2 2 292 39 363 6.44e-106 313
MS.gene054991.t1 MTR_7g066890 52.469 324 120 2 3 292 142 465 1.30e-104 313
MS.gene054991.t1 MTR_8g098880 37.209 172 74 2 36 173 90 261 5.00e-30 115
MS.gene054991.t1 MTR_8g098880 44.737 76 32 1 32 107 45 110 3.57e-12 65.9
MS.gene054991.t1 MTR_7g066920 64.384 73 26 0 109 181 1 73 7.89e-27 101
MS.gene054991.t1 MTR_7g067580 34.091 176 81 2 33 173 9 184 4.00e-25 100
MS.gene054991.t1 MTR_7g066970 37.755 196 85 2 5 173 66 251 5.35e-23 96.3
MS.gene054991.t1 MTR_7g078120 28.889 225 130 6 78 274 312 534 1.27e-15 77.4
MS.gene054991.t1 MTR_7g078120 28.889 225 130 6 78 274 312 534 1.64e-15 77.0
MS.gene054991.t1 MTR_8g028905 26.064 188 108 3 137 293 389 576 1.86e-13 70.9
MS.gene054991.t1 MTR_1g073380 26.582 158 90 1 152 283 394 551 3.26e-13 70.1
MS.gene054991.t1 MTR_8g098645 26.286 175 103 1 142 290 132 306 7.90e-13 68.2
MS.gene054991.t1 MTR_1g073530 25.000 164 97 1 153 290 385 548 1.14e-11 65.5
MS.gene054991.t1 MTR_1g073590 25.000 164 97 1 153 290 382 545 1.24e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054991.t1 AT5G04110 31.892 185 90 3 132 280 341 525 1.82e-24 103
MS.gene054991.t1 AT5G04110 31.892 185 90 3 132 280 340 524 1.83e-24 103
MS.gene054991.t1 AT5G04110 31.892 185 90 3 132 280 340 524 1.83e-24 103
MS.gene054991.t1 AT5G04110 31.515 165 77 3 132 260 340 504 1.25e-18 86.3

Find 55 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACTTTGATGATTCTATTA+AGG 0.192630 7.4:+36713013 MS.gene054991:CDS
TATCCATGCTAGAGATTTAA+TGG 0.197752 7.4:+36713070 MS.gene054991:CDS
TTCAAGCTGAAGTTCGTAAA+TGG 0.225040 7.4:+36713240 MS.gene054991:CDS
TTAACACAATTAACTAATTC+AGG 0.246830 7.4:-36713317 None:intergenic
TGAGTTCCTTCTTTGATCTT+TGG 0.253488 7.4:-36712865 None:intergenic
GACTTTGATGATTCTATTAA+GGG 0.305104 7.4:+36713014 MS.gene054991:CDS
ACTTACCATCATTTATGTAT+TGG 0.365830 7.4:-36712791 None:intergenic
CTAGGTCCAATGGGAATAAC+TGG 0.367656 7.4:-36713215 None:intergenic
GTCTCTATACTCATACATCT+AGG 0.386269 7.4:-36713587 None:intergenic
TCATCATGCTAGTCATAGAT+TGG 0.411194 7.4:-36713541 None:intergenic
GAGATTTAATGGCGAGTAAT+CGG 0.415393 7.4:+36713081 MS.gene054991:CDS
AAGAGTATGCTTTAAAATCA+TGG 0.434500 7.4:+36713420 MS.gene054991:CDS
CCAATGGGAATAACTGGTCT+TGG 0.436450 7.4:-36713209 None:intergenic
TCAAGCTGAAGTTCGTAAAT+GGG 0.444773 7.4:+36713241 MS.gene054991:CDS
CGAACTTCAGCTTGAAACCT+AGG 0.456711 7.4:-36713233 None:intergenic
AGCTAGGGAATGCTTAAAAT+CGG 0.459249 7.4:+36713355 MS.gene054991:CDS
AAACTTCCGACTTGAATAAC+CGG 0.459854 7.4:-36713387 None:intergenic
CCAAGACCAGTTATTCCCAT+TGG 0.466479 7.4:+36713209 MS.gene054991:CDS
TCTATCATGTTCTGCACTAT+CGG 0.487951 7.4:+36712896 MS.gene054991:CDS
AATGACAATGCAAAGTTATG+TGG 0.501210 7.4:+36712960 MS.gene054991:CDS
CATCATGCTAGTCATAGATT+GGG 0.502282 7.4:-36713540 None:intergenic
GCGAGTAATCGGTATTTGTA+TGG 0.503013 7.4:+36713092 MS.gene054991:CDS
TTGCATTGTCATTTAGAGAA+GGG 0.520249 7.4:-36712950 None:intergenic
ATCGGTAAAGAGAAATATAG+TGG 0.522648 7.4:+36712834 MS.gene054991:CDS
TTTAACCAATACATAAATGA+TGG 0.525857 7.4:+36712786 MS.gene054991:CDS
TAGAATCATCAAAGTCACTT+AGG 0.526045 7.4:-36713007 None:intergenic
ATAGAGACAAGATCTCCCTT+TGG 0.528050 7.4:+36713602 MS.gene054991:CDS
AGCTTGAAACCTAGGTCCAA+TGG 0.530844 7.4:-36713225 None:intergenic
GAATAACTGGTCTTGGAAGA+TGG 0.535998 7.4:-36713202 None:intergenic
GCTTGAAACCTAGGTCCAAT+GGG 0.536651 7.4:-36713224 None:intergenic
TGGAAGATGGTTACCCCTAG+AGG 0.537765 7.4:-36713189 None:intergenic
TTTGCATTGTCATTTAGAGA+AGG 0.540742 7.4:-36712951 None:intergenic
CAGTTATTCCCATTGGACCT+AGG 0.541236 7.4:+36713216 MS.gene054991:CDS
AAAATGCCAAAGATCAAAGA+AGG 0.541347 7.4:+36712859 MS.gene054991:CDS
AAAACACATCGGTGAAGCTA+GGG 0.541382 7.4:+36713340 MS.gene054991:CDS
TTCACGATCAACTGCACCAA+AGG 0.541774 7.4:-36713618 None:intergenic
TGCATTGTCATTTAGAGAAG+GGG 0.543570 7.4:-36712949 None:intergenic
TGGTATCGAGACATCAATAG+AGG 0.552190 7.4:+36713112 MS.gene054991:CDS
TTAAAATCGGAGATTGATAC+CGG 0.555035 7.4:+36713368 MS.gene054991:CDS
TTGATACCGGTTATTCAAGT+CGG 0.555858 7.4:+36713381 MS.gene054991:CDS
TCATGTTCTGCACTATCGGA+TGG 0.557424 7.4:+36712900 MS.gene054991:CDS
TCTCTATACTCATACATCTA+GGG 0.558185 7.4:-36713586 None:intergenic
GATAGTGCAGAACATGATAG+AGG 0.563241 7.4:-36712894 None:intergenic
TCACGATCAACTGCACCAAA+GGG 0.567200 7.4:-36713617 None:intergenic
AATAGAGGAGAAATCGAAAG+AGG 0.567267 7.4:+36713127 MS.gene054991:CDS
GAGCATAGCATGTTTCATGT+TGG 0.569427 7.4:-36713273 None:intergenic
TCGCCATTAAATCTCTAGCA+TGG 0.577892 7.4:-36713073 None:intergenic
GAAATCGAAAGAGGAAGACA+TGG 0.578703 7.4:+36713136 MS.gene054991:CDS
GCATTGTCATTTAGAGAAGG+GGG 0.603152 7.4:-36712948 None:intergenic
ATGCTAGTCATAGATTGGGA+TGG 0.609590 7.4:-36713536 None:intergenic
GGAAGATGGTTACCCCTAGA+GGG 0.610440 7.4:-36713188 None:intergenic
GCTGAAGTTCGTAAATGGGA+TGG 0.617058 7.4:+36713245 MS.gene054991:CDS
GCACTATCGGATGGAACACA+AGG 0.625934 7.4:+36712909 MS.gene054991:CDS
TGCTTTAAAATCATGGACAG+TGG 0.644009 7.4:+36713427 MS.gene054991:CDS
TAAAGCTATCTTGATTCACA+AGG 0.651202 7.4:-36713046 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAGTATAATTTTCTAAAA+AGG + chr7.4:36712642-36712661 MS.gene054991:intron 10.0%
!!! AGAAAATAATTTTGATTCTT+AGG - chr7.4:36712733-36712752 None:intergenic 15.0%
!!! TATAATTTTCTAAAAAGGTA+AGG + chr7.4:36712647-36712666 MS.gene054991:intron 15.0%
!! AATTGTGTTAAAAAACACAT+CGG + chr7.4:36713329-36713348 MS.gene054991:CDS 20.0%
!! TTAACACAATTAACTAATTC+AGG - chr7.4:36713320-36713339 None:intergenic 20.0%
!! TTTAACCAATACATAAATGA+TGG + chr7.4:36712786-36712805 MS.gene054991:CDS 20.0%
!!! TTTTTTTTGTATATCAGAAG+AGG + chr7.4:36712760-36712779 MS.gene054991:intron 20.0%
! ACTTACCATCATTTATGTAT+TGG - chr7.4:36712794-36712813 None:intergenic 25.0%
!! AAAAGGTAAGGATTAACTTT+TGG + chr7.4:36712659-36712678 MS.gene054991:intron 25.0%
!! GACTTTGATGATTCTATTAA+GGG + chr7.4:36713014-36713033 MS.gene054991:CDS 25.0%
!! TGACTTTGATGATTCTATTA+AGG + chr7.4:36713013-36713032 MS.gene054991:CDS 25.0%
!!! AAGAGTATGCTTTAAAATCA+TGG + chr7.4:36713420-36713439 MS.gene054991:CDS 25.0%
AAAATGCCAAAGATCAAAGA+AGG + chr7.4:36712859-36712878 MS.gene054991:CDS 30.0%
AATGACAATGCAAAGTTATG+TGG + chr7.4:36712960-36712979 MS.gene054991:CDS 30.0%
ATCGGTAAAGAGAAATATAG+TGG + chr7.4:36712834-36712853 MS.gene054991:CDS 30.0%
TAAAGCTATCTTGATTCACA+AGG - chr7.4:36713049-36713068 None:intergenic 30.0%
TAGAATCATCAAAGTCACTT+AGG - chr7.4:36713010-36713029 None:intergenic 30.0%
TATCCATGCTAGAGATTTAA+TGG + chr7.4:36713070-36713089 MS.gene054991:CDS 30.0%
TCTCTATACTCATACATCTA+GGG - chr7.4:36713589-36713608 None:intergenic 30.0%
TTAAAATCGGAGATTGATAC+CGG + chr7.4:36713368-36713387 MS.gene054991:CDS 30.0%
! TTGCATTGTCATTTAGAGAA+GGG - chr7.4:36712953-36712972 None:intergenic 30.0%
! TTTGCATTGTCATTTAGAGA+AGG - chr7.4:36712954-36712973 None:intergenic 30.0%
AATAGAGGAGAAATCGAAAG+AGG + chr7.4:36713127-36713146 MS.gene054991:CDS 35.0%
AGCTAGGGAATGCTTAAAAT+CGG + chr7.4:36713355-36713374 MS.gene054991:CDS 35.0%
CATCATGCTAGTCATAGATT+GGG - chr7.4:36713543-36713562 None:intergenic 35.0%
GAGATTTAATGGCGAGTAAT+CGG + chr7.4:36713081-36713100 MS.gene054991:CDS 35.0%
GTCTCTATACTCATACATCT+AGG - chr7.4:36713590-36713609 None:intergenic 35.0%
TCAAGCTGAAGTTCGTAAAT+GGG + chr7.4:36713241-36713260 MS.gene054991:CDS 35.0%
TCATCATGCTAGTCATAGAT+TGG - chr7.4:36713544-36713563 None:intergenic 35.0%
TCTATCATGTTCTGCACTAT+CGG + chr7.4:36712896-36712915 MS.gene054991:CDS 35.0%
TGAGTTCCTTCTTTGATCTT+TGG - chr7.4:36712868-36712887 None:intergenic 35.0%
TTCAAGCTGAAGTTCGTAAA+TGG + chr7.4:36713240-36713259 MS.gene054991:CDS 35.0%
! AAACTTCCGACTTGAATAAC+CGG - chr7.4:36713390-36713409 None:intergenic 35.0%
! AAGAACTTTTTACCCTCTAG+GGG + chr7.4:36713176-36713195 MS.gene054991:CDS 35.0%
! CAAGAACTTTTTACCCTCTA+GGG + chr7.4:36713175-36713194 MS.gene054991:CDS 35.0%
! TCAAGAACTTTTTACCCTCT+AGG + chr7.4:36713174-36713193 MS.gene054991:CDS 35.0%
! TGCATTGTCATTTAGAGAAG+GGG - chr7.4:36712952-36712971 None:intergenic 35.0%
! TTGATACCGGTTATTCAAGT+CGG + chr7.4:36713381-36713400 MS.gene054991:CDS 35.0%
!! TGCTTTAAAATCATGGACAG+TGG + chr7.4:36713427-36713446 MS.gene054991:CDS 35.0%
!!! GCTACTAGTTTTGAACTCAT+CGG + chr7.4:36712816-36712835 MS.gene054991:CDS 35.0%
AAAAACACATCGGTGAAGCT+AGG + chr7.4:36713339-36713358 MS.gene054991:CDS 40.0%
AAAACACATCGGTGAAGCTA+GGG + chr7.4:36713340-36713359 MS.gene054991:CDS 40.0%
ATAGAGACAAGATCTCCCTT+TGG + chr7.4:36713602-36713621 MS.gene054991:CDS 40.0%
ATGCTAGTCATAGATTGGGA+TGG - chr7.4:36713539-36713558 None:intergenic 40.0%
GAAATCGAAAGAGGAAGACA+TGG + chr7.4:36713136-36713155 MS.gene054991:CDS 40.0%
GAGCATAGCATGTTTCATGT+TGG - chr7.4:36713276-36713295 None:intergenic 40.0%
GATAGTGCAGAACATGATAG+AGG - chr7.4:36712897-36712916 None:intergenic 40.0%
GCGAGTAATCGGTATTTGTA+TGG + chr7.4:36713092-36713111 MS.gene054991:CDS 40.0%
TCGCCATTAAATCTCTAGCA+TGG - chr7.4:36713076-36713095 None:intergenic 40.0%
! AGAATTTTCGAAGCTCGTCA+TGG + chr7.4:36713505-36713524 MS.gene054991:CDS 40.0%
! GCATTGTCATTTAGAGAAGG+GGG - chr7.4:36712951-36712970 None:intergenic 40.0%
! TGGTATCGAGACATCAATAG+AGG + chr7.4:36713112-36713131 MS.gene054991:CDS 40.0%
!! GAATAACTGGTCTTGGAAGA+TGG - chr7.4:36713205-36713224 None:intergenic 40.0%
AGCTTGAAACCTAGGTCCAA+TGG - chr7.4:36713228-36713247 None:intergenic 45.0%
CAGTTATTCCCATTGGACCT+AGG + chr7.4:36713216-36713235 MS.gene054991:CDS 45.0%
CCAAGACCAGTTATTCCCAT+TGG + chr7.4:36713209-36713228 MS.gene054991:CDS 45.0%
CGAACTTCAGCTTGAAACCT+AGG - chr7.4:36713236-36713255 None:intergenic 45.0%
CTAGGTCCAATGGGAATAAC+TGG - chr7.4:36713218-36713237 None:intergenic 45.0%
GCTGAAGTTCGTAAATGGGA+TGG + chr7.4:36713245-36713264 MS.gene054991:CDS 45.0%
GCTTGAAACCTAGGTCCAAT+GGG - chr7.4:36713227-36713246 None:intergenic 45.0%
TCACGATCAACTGCACCAAA+GGG - chr7.4:36713620-36713639 None:intergenic 45.0%
TCATGTTCTGCACTATCGGA+TGG + chr7.4:36712900-36712919 MS.gene054991:CDS 45.0%
TTCACGATCAACTGCACCAA+AGG - chr7.4:36713621-36713640 None:intergenic 45.0%
! CCAATGGGAATAACTGGTCT+TGG - chr7.4:36713212-36713231 None:intergenic 45.0%
GCACTATCGGATGGAACACA+AGG + chr7.4:36712909-36712928 MS.gene054991:CDS 50.0%
GGAAGATGGTTACCCCTAGA+GGG - chr7.4:36713191-36713210 None:intergenic 50.0%
TGGAAGATGGTTACCCCTAG+AGG - chr7.4:36713192-36713211 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 36712587 36713655 36712587 ID=MS.gene054991
chr7.4 mRNA 36712587 36713655 36712587 ID=MS.gene054991.t1;Parent=MS.gene054991
chr7.4 exon 36712587 36712598 36712587 ID=MS.gene054991.t1.exon1;Parent=MS.gene054991.t1
chr7.4 CDS 36712587 36712598 36712587 ID=cds.MS.gene054991.t1;Parent=MS.gene054991.t1
chr7.4 exon 36712777 36713655 36712777 ID=MS.gene054991.t1.exon2;Parent=MS.gene054991.t1
chr7.4 CDS 36712777 36713655 36712777 ID=cds.MS.gene054991.t1;Parent=MS.gene054991.t1
Gene Sequence

>MS.gene054991

ATGATTTCAAAAGTAAGGATTAACTTTTGATAATACAAACTCTAACAATGTTAATTAAAGTATAATTTTCTAAAAAGGTAAGGATTAACTTTTGGTAATATAATTTTCTAAAAAGTGTATAGCTTCATAAATAGTTTTATACTCCTAAGAATCAAAATTATTTTCTTTTTTTTTTTTTTTTGTATATCAGAAGAGGAAATTTAACCAATACATAAATGATGGTAAGTGCGCTACTAGTTTTGAACTCATCGGTAAAGAGAAATATAGTGGTGAAAATGCCAAAGATCAAAGAAGGAACTCATCTTATCCTCTATCATGTTCTGCACTATCGGATGGAACACAAGGAACACAAGAATATGTTCCCCCTTCTCTAAATGACAATGCAAAGTTATGTGGTATTTCTACACAAGACTGCTCTCACCTAAGTGACTTTGATGATTCTATTAAGGGTTCTAGTTACCTTGTGAATCAAGATAGCTTTAATATCCATGCTAGAGATTTAATGGCGAGTAATCGGTATTTGTATGGTATCGAGACATCAATAGAGGAGAAATCGAAAGAGGAAGACATGGAAGTCATTAACTCACTCAAGAACTTTTTACCCTCTAGGGGTAACCATCTTCCAAGACCAGTTATTCCCATTGGACCTAGGTTTCAAGCTGAAGTTCGTAAATGGGATGGCAAAGCCAACATGAAACATGCTATGCTCAACTCATGCACGTGTGACAATCCTGAATTAGTTAATTGTGTTAAAAAACACATCGGTGAAGCTAGGGAATGCTTAAAATCGGAGATTGATACCGGTTATTCAAGTCGGAAGTTTGATGACATAGAAGAGTATGCTTTAAAATCATGGACAGTGGAAGAGCAAAAGAAATTTAAATCTCTAAAAAATTTGAATTTGCTATCAAGTTACACAGAATTTTCGAAGCTCGTCATGGAGTACTTTCCATCCCAATCTATGACTAGCATGATGAATTACTATTATAATGTATACATCCCTAGATGTATGAGTATAGAGACAAGATCTCCCTTTGGTGCAGTTGATCGTGAATCAAAATTGAACTAG

Protein sequence

>MS.gene054991.t1

MISKKRKFNQYINDGKCATSFELIGKEKYSGENAKDQRRNSSYPLSCSALSDGTQGTQEYVPPSLNDNAKLCGISTQDCSHLSDFDDSIKGSSYLVNQDSFNIHARDLMASNRYLYGIETSIEEKSKEEDMEVINSLKNFLPSRGNHLPRPVIPIGPRFQAEVRKWDGKANMKHAMLNSCTCDNPELVNCVKKHIGEARECLKSEIDTGYSSRKFDDIEEYALKSWTVEEQKKFKSLKNLNLLSSYTEFSKLVMEYFPSQSMTSMMNYYYNVYIPRCMSIETRSPFGAVDRESKLN