AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06395

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06395.t1 MTR_3g096400 94.712 208 9 2 1 206 1 208 6.62e-143 397
MS.gene06395.t1 MTR_8g093790 57.843 204 58 9 5 206 8 185 4.86e-65 199
MS.gene06395.t1 MTR_4g094302 42.500 200 94 6 7 205 11 190 1.65e-46 152
MS.gene06395.t1 MTR_4g094302 49.180 122 59 3 7 127 11 130 1.23e-37 128
MS.gene06395.t1 MTR_5g041940 32.864 213 119 7 5 201 1 205 8.34e-30 111
MS.gene06395.t1 MTR_4g081870 37.888 161 84 6 6 158 8 160 3.06e-27 104
MS.gene06395.t1 MTR_2g080010 36.810 163 90 5 7 158 15 175 5.20e-27 105
MS.gene06395.t1 MTR_7g100990 36.810 163 87 6 5 158 9 164 7.45e-26 102
MS.gene06395.t1 MTR_4g108760 40.373 161 76 7 7 158 17 166 1.34e-25 102
MS.gene06395.t1 MTR_4g089135 34.286 175 90 5 7 158 15 187 3.23e-25 100
MS.gene06395.t1 MTR_2157s0010 31.677 161 100 3 7 158 8 167 3.53e-25 99.4
MS.gene06395.t1 MTR_3g435150 37.654 162 87 4 3 158 12 165 4.59e-25 99.8
MS.gene06395.t1 MTR_3g109340 40.373 161 74 6 7 158 22 169 1.59e-24 99.0
MS.gene06395.t1 MTR_2g079990 33.125 160 91 3 5 158 14 163 5.06e-24 97.8
MS.gene06395.t1 MTR_2g093810 36.420 162 81 7 7 156 14 165 5.88e-24 97.1
MS.gene06395.t1 MTR_3g109340 39.130 161 77 6 7 158 22 170 6.00e-24 97.4
MS.gene06395.t1 MTR_3g098810 32.515 163 96 4 4 157 11 168 9.53e-24 94.7
MS.gene06395.t1 MTR_2g062730 32.317 164 89 5 5 158 8 159 1.39e-23 96.3
MS.gene06395.t1 MTR_3g088110 32.515 163 96 4 1 157 1 155 1.58e-23 95.1
MS.gene06395.t1 MTR_3g070030 35.795 176 95 6 5 172 17 182 1.83e-23 96.3
MS.gene06395.t1 MTR_8g094580 32.121 165 95 4 1 157 1 156 2.72e-23 94.7
MS.gene06395.t1 MTR_1g008740 40.000 165 71 8 7 158 27 176 3.17e-23 95.5
MS.gene06395.t1 MTR_1g008740 38.547 179 80 9 7 170 13 176 3.67e-23 95.1
MS.gene06395.t1 MTR_7g005280 35.185 162 80 5 5 157 13 158 5.74e-23 93.2
MS.gene06395.t1 MTR_6g084430 37.500 160 78 6 7 158 5 150 1.43e-22 93.2
MS.gene06395.t1 MTR_3g064580 32.022 178 104 4 5 174 4 172 1.76e-22 95.1
MS.gene06395.t1 MTR_8g059170 32.022 178 105 4 5 176 12 179 1.94e-22 93.2
MS.gene06395.t1 MTR_8g099750 30.625 160 98 4 4 156 11 164 7.97e-22 89.4
MS.gene06395.t1 MTR_2g079990 30.726 179 98 4 5 158 14 191 1.30e-21 91.3
MS.gene06395.t1 MTR_7g085220 32.370 173 94 7 5 161 6 171 1.63e-21 90.5
MS.gene06395.t1 MTR_4g035590 32.222 180 97 7 7 175 8 173 2.14e-21 90.5
MS.gene06395.t1 MTR_2g078700 36.646 161 84 6 7 158 23 174 2.71e-21 90.5
MS.gene06395.t1 MTR_3g096920 31.447 159 95 4 5 157 5 155 3.83e-21 89.0
MS.gene06395.t1 MTR_2g064470 34.161 161 85 6 5 157 9 156 4.11e-21 89.0
MS.gene06395.t1 MTR_6g477900 36.641 131 74 3 5 127 4 133 6.32e-21 87.4
MS.gene06395.t1 MTR_8g024480 30.247 162 103 3 6 158 14 174 6.48e-21 89.7
MS.gene06395.t1 MTR_3g098810 34.307 137 79 3 4 131 11 145 8.89e-21 85.5
MS.gene06395.t1 MTR_7g085260 31.132 212 127 5 7 206 17 221 1.09e-20 89.0
MS.gene06395.t1 MTR_5g076850 32.787 183 105 5 5 179 4 176 1.40e-20 89.4
MS.gene06395.t1 MTR_8g467490 31.214 173 105 5 9 171 16 184 1.72e-20 88.6
MS.gene06395.t1 MTR_5g012080 29.341 167 99 4 1 158 1 157 2.37e-20 87.4
MS.gene06395.t1 MTR_1g069805 29.714 175 98 4 5 167 8 169 5.49e-20 85.5
MS.gene06395.t1 MTR_3g096140 30.846 201 115 8 1 189 1 189 8.57e-20 85.1
MS.gene06395.t1 MTR_8g093580 34.146 164 88 5 4 158 3 155 2.57e-19 85.5
MS.gene06395.t1 MTR_3g096140 30.846 201 115 8 1 189 1 189 2.58e-19 84.7
MS.gene06395.t1 MTR_4g101680 31.319 182 102 6 1 173 1 168 3.01e-19 84.7
MS.gene06395.t1 MTR_3g070040 33.140 172 102 4 5 171 6 169 3.91e-19 84.0
MS.gene06395.t1 MTR_7g097090 30.675 163 87 4 5 157 8 154 4.85e-19 83.2
MS.gene06395.t1 MTR_6g011860 29.114 158 106 1 7 158 15 172 1.12e-18 83.2
MS.gene06395.t1 MTR_2g064090 29.630 162 97 3 5 158 4 156 1.33e-18 82.8
MS.gene06395.t1 MTR_7g011120 32.530 166 93 4 5 158 6 164 1.33e-18 82.4
MS.gene06395.t1 MTR_6g012670 32.704 159 93 4 6 158 19 169 1.92e-18 82.0
MS.gene06395.t1 MTR_4g036030 30.178 169 93 6 1 158 1 155 2.37e-18 82.0
MS.gene06395.t1 MTR_5g040420 31.214 173 104 6 9 171 16 183 2.68e-18 82.4
MS.gene06395.t1 MTR_1g090720 32.353 170 102 5 7 168 42 206 3.89e-18 82.0
MS.gene06395.t1 MTR_1g096430 29.012 162 92 6 7 158 7 155 6.90e-18 80.9
MS.gene06395.t1 MTR_2g086690 31.395 172 95 7 1 159 1 162 8.76e-18 79.3
MS.gene06395.t1 MTR_2g068880 29.878 164 99 5 9 158 24 185 9.44e-18 80.1
MS.gene06395.t1 MTR_5g014300 27.835 194 114 3 5 184 4 185 1.22e-17 80.1
MS.gene06395.t1 MTR_8g063550 33.742 163 90 5 4 158 3 155 1.29e-17 80.5
MS.gene06395.t1 MTR_3g093050 33.750 160 88 4 7 158 22 171 1.43e-17 80.5
MS.gene06395.t1 MTR_8g023930 36.154 130 72 4 7 126 24 152 1.49e-17 80.5
MS.gene06395.t1 MTR_2g086880 32.540 126 77 2 9 127 20 144 1.53e-17 80.5
MS.gene06395.t1 MTR_3g116070 35.252 139 82 3 7 137 23 161 1.54e-17 79.7
MS.gene06395.t1 MTR_8g063550 31.795 195 108 8 4 184 3 186 1.66e-17 79.7
MS.gene06395.t1 MTR_2g086880 32.540 126 77 2 9 127 20 144 1.78e-17 80.5
MS.gene06395.t1 MTR_3g093050 33.750 160 88 4 7 158 22 171 1.90e-17 80.5
MS.gene06395.t1 MTR_7g011130 28.378 222 131 5 5 205 17 231 2.19e-17 79.3
MS.gene06395.t1 MTR_5g069030 32.919 161 90 5 6 158 24 174 2.27e-17 80.1
MS.gene06395.t1 MTR_8g063550 31.795 195 108 8 4 184 3 186 2.47e-17 79.7
MS.gene06395.t1 MTR_4g052620 30.000 170 100 5 1 159 1 162 2.62e-17 78.2
MS.gene06395.t1 MTR_2g068920 29.193 161 100 4 9 156 23 182 2.79e-17 78.6
MS.gene06395.t1 MTR_5g090970 27.673 159 105 4 9 158 20 177 3.63e-17 78.6
MS.gene06395.t1 MTR_8g023840 33.129 163 78 8 7 156 11 155 6.05e-17 78.6
MS.gene06395.t1 MTR_5g021710 28.221 163 92 6 7 158 8 156 8.74e-17 77.8
MS.gene06395.t1 MTR_1g090723 31.250 160 89 7 9 158 10 158 1.08e-16 78.2
MS.gene06395.t1 MTR_3g096920 30.534 131 83 2 5 129 5 133 1.56e-16 76.6
MS.gene06395.t1 MTR_3g093040 31.288 163 94 4 4 158 105 257 1.66e-16 76.6
MS.gene06395.t1 MTR_8g076110 30.534 131 79 4 7 127 6 134 1.97e-16 76.3
MS.gene06395.t1 MTR_1g090723 30.000 160 90 6 9 158 10 157 2.71e-16 77.0
MS.gene06395.t1 MTR_1g090723 30.000 160 90 6 9 158 10 157 2.81e-16 77.0
MS.gene06395.t1 MTR_6g032770 33.594 128 78 2 7 127 18 145 3.43e-16 76.3
MS.gene06395.t1 MTR_4g098630 30.357 168 96 5 5 162 4 160 7.06e-16 74.7
MS.gene06395.t1 MTR_4g134460 30.128 156 92 3 9 158 56 200 7.12e-16 75.5
MS.gene06395.t1 MTR_8g023900 30.357 168 81 7 7 156 188 337 7.58e-16 75.5
MS.gene06395.t1 MTR_5g090970 28.571 161 100 6 9 158 20 176 9.55e-16 74.3
MS.gene06395.t1 MTR_0036s0150 30.435 161 88 7 9 158 7 154 1.40e-15 74.7
MS.gene06395.t1 MTR_1g097300 30.709 127 79 2 9 127 21 146 3.19e-13 68.2
MS.gene06395.t1 MTR_7g105170 24.762 210 130 7 7 195 4 206 6.68e-13 65.9
MS.gene06395.t1 MTR_3g096140 28.889 180 104 8 22 189 1 168 2.50e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06395.t1 AT5G64530 42.995 207 92 6 5 205 1 187 3.41e-44 146
MS.gene06395.t1 AT5G64530 50.806 124 54 3 5 127 1 118 5.24e-35 121
MS.gene06395.t1 AT1G61110 36.257 171 94 4 7 165 16 183 3.50e-29 110
MS.gene06395.t1 AT2G24430 40.000 160 77 5 7 158 16 164 1.51e-26 103
MS.gene06395.t1 AT2G24430 40.000 160 77 5 7 158 16 164 1.51e-26 103
MS.gene06395.t1 AT1G01720 36.364 165 88 6 1 157 1 156 5.74e-26 101
MS.gene06395.t1 AT5G07680 39.623 159 78 6 7 157 3 151 7.77e-26 102
MS.gene06395.t1 AT5G07680 39.623 159 78 6 7 157 17 165 8.69e-26 102
MS.gene06395.t1 AT3G04070 35.976 164 89 5 7 158 10 169 5.70e-25 100
MS.gene06395.t1 AT3G04060 37.805 164 83 6 3 157 16 169 7.17e-25 99.8
MS.gene06395.t1 AT1G52890 33.750 160 90 3 5 158 12 161 1.85e-24 98.2
MS.gene06395.t1 AT1G69490 34.591 159 90 5 7 158 9 160 1.91e-24 97.4
MS.gene06395.t1 AT3G18400 38.750 160 77 5 7 158 5 151 2.79e-24 97.8
MS.gene06395.t1 AT3G04070 34.270 178 89 6 7 158 10 185 4.27e-24 98.2
MS.gene06395.t1 AT5G39610 35.366 164 84 4 5 158 18 169 4.47e-24 96.7
MS.gene06395.t1 AT1G77450 33.951 162 93 4 2 157 5 158 5.74e-24 95.9
MS.gene06395.t1 AT5G61430 37.107 159 84 5 7 158 16 165 7.82e-24 97.1
MS.gene06395.t1 AT4G27410 33.125 160 91 4 5 158 12 161 8.69e-24 96.3
MS.gene06395.t1 AT5G18270 36.196 163 87 5 3 157 17 170 1.57e-23 96.3
MS.gene06395.t1 AT5G08790 28.155 206 133 4 1 197 1 200 1.74e-23 95.1
MS.gene06395.t1 AT5G18270 35.583 163 88 5 3 157 17 170 2.67e-23 95.5
MS.gene06395.t1 AT3G15500 32.500 160 92 3 5 158 12 161 3.65e-23 94.7
MS.gene06395.t1 AT5G22290 36.029 136 79 3 9 137 23 157 8.66e-23 94.4
MS.gene06395.t1 AT5G46590 34.177 158 88 3 7 156 6 155 9.96e-23 93.2
MS.gene06395.t1 AT5G53950 37.349 166 86 6 2 158 12 168 1.75e-22 94.0
MS.gene06395.t1 AT3G15510 33.962 159 92 5 11 158 21 177 3.82e-22 92.8
MS.gene06395.t1 AT4G10350 31.000 200 112 7 7 191 9 197 4.30e-22 92.4
MS.gene06395.t1 AT3G61910 32.571 175 99 6 6 163 10 182 6.80e-22 91.7
MS.gene06395.t1 AT1G76420 37.423 163 75 7 7 157 22 169 1.30e-21 90.9
MS.gene06395.t1 AT3G10500 33.333 165 92 4 2 158 4 158 1.35e-21 92.4
MS.gene06395.t1 AT5G63790 32.164 171 100 5 1 163 44 206 1.45e-21 90.5
MS.gene06395.t1 AT5G63790 32.164 171 100 5 1 163 54 216 1.71e-21 90.5
MS.gene06395.t1 AT1G79580 31.138 167 92 5 2 158 12 165 4.06e-21 90.1
MS.gene06395.t1 AT1G79580 31.138 167 92 5 2 158 12 165 4.06e-21 90.1
MS.gene06395.t1 AT1G79580 31.138 167 92 5 2 158 12 165 4.06e-21 90.1
MS.gene06395.t1 AT1G79580 31.138 167 92 5 2 158 12 165 4.06e-21 90.1
MS.gene06395.t1 AT1G79580 31.138 167 92 5 2 158 12 165 4.06e-21 90.1
MS.gene06395.t1 AT3G44290 34.848 132 78 3 9 133 16 146 5.11e-21 87.4
MS.gene06395.t1 AT5G46590 36.154 130 75 2 7 128 6 135 6.87e-21 88.2
MS.gene06395.t1 AT3G12977 31.098 164 98 4 5 162 16 170 7.94e-21 87.8
MS.gene06395.t1 AT2G33480 34.783 161 84 6 4 157 12 158 9.22e-21 87.4
MS.gene06395.t1 AT5G04410 32.727 165 93 4 2 158 4 158 1.04e-20 89.7
MS.gene06395.t1 AT5G17260 32.716 162 92 3 5 158 4 156 1.04e-20 89.7
MS.gene06395.t1 AT4G27410 32.203 177 87 6 5 158 12 178 1.15e-20 88.2
MS.gene06395.t1 AT3G17730 35.878 131 75 3 5 127 4 133 1.48e-20 86.7
MS.gene06395.t1 AT2G46770 30.814 172 104 6 6 164 15 184 1.51e-20 88.2
MS.gene06395.t1 AT3G44290 34.848 132 78 3 9 133 16 146 1.88e-20 87.8
MS.gene06395.t1 AT5G13180 32.927 164 84 6 4 157 11 158 4.53e-20 85.5
MS.gene06395.t1 AT3G15170 35.669 157 87 4 7 156 20 169 4.84e-20 86.3
MS.gene06395.t1 AT1G33280 31.138 167 92 5 7 164 8 160 5.20e-20 86.3
MS.gene06395.t1 AT3G29035 40.458 131 67 4 5 127 22 149 7.15e-20 85.9
MS.gene06395.t1 AT4G35580 31.633 196 100 6 7 183 9 189 1.24e-19 86.7
MS.gene06395.t1 AT2G33480 34.161 161 83 6 4 157 12 156 1.30e-19 84.3
MS.gene06395.t1 AT4G35580 31.633 196 100 6 7 183 9 189 1.73e-19 86.3
MS.gene06395.t1 AT4G35580 31.633 196 100 6 7 183 9 189 1.75e-19 86.3
MS.gene06395.t1 AT1G32510 30.488 164 92 3 7 156 6 161 2.61e-19 84.0
MS.gene06395.t1 AT1G56010 31.677 161 94 5 5 157 17 169 2.68e-19 84.3
MS.gene06395.t1 AT1G65910 32.716 162 91 4 5 158 4 155 4.33e-19 85.1
MS.gene06395.t1 AT3G03200 30.864 162 95 3 5 158 4 156 5.14e-19 84.7
MS.gene06395.t1 AT1G32770 30.120 166 99 5 6 158 15 176 5.88e-19 84.0
MS.gene06395.t1 AT2G02450 28.421 190 115 3 9 188 53 231 6.64e-19 84.0
MS.gene06395.t1 AT1G12260 27.650 217 124 8 1 205 1 196 7.36e-19 83.6
MS.gene06395.t1 AT2G02450 28.421 190 115 3 9 188 53 231 9.61e-19 83.6
MS.gene06395.t1 AT5G62380 29.825 171 91 7 1 158 1 155 1.15e-18 83.2
MS.gene06395.t1 AT5G62380 29.825 171 91 7 1 158 1 155 1.15e-18 83.2
MS.gene06395.t1 AT1G12260 27.650 217 124 8 1 205 1 196 1.41e-18 83.2
MS.gene06395.t1 AT2G43000 28.205 156 97 3 9 158 20 166 1.81e-18 81.6
MS.gene06395.t1 AT5G24590 30.061 163 97 4 4 158 10 163 2.30e-18 82.8
MS.gene06395.t1 AT4G17980 32.727 165 93 5 7 162 6 161 2.59e-18 80.9
MS.gene06395.t1 AT1G52880 31.138 167 85 6 11 158 21 176 3.11e-18 81.6
MS.gene06395.t1 AT4G17980 32.704 159 91 4 7 156 6 157 3.42e-18 80.9
MS.gene06395.t1 AT1G33060 32.704 159 89 4 7 157 24 172 6.84e-18 81.6
MS.gene06395.t1 AT1G33060 32.704 159 89 4 7 157 24 172 7.12e-18 81.6
MS.gene06395.t1 AT2G27300 34.351 131 78 3 4 127 11 140 9.53e-18 80.5
MS.gene06395.t1 AT2G18060 29.012 162 92 6 7 158 9 157 1.14e-17 80.5
MS.gene06395.t1 AT2G18060 29.012 162 92 6 7 158 9 157 1.14e-17 80.5
MS.gene06395.t1 AT4G36160 28.395 162 93 5 7 158 14 162 1.94e-17 79.7
MS.gene06395.t1 AT4G36160 28.395 162 93 5 7 158 10 158 2.16e-17 79.7
MS.gene06395.t1 AT4G36160 28.395 162 93 5 7 158 10 158 2.16e-17 79.7
MS.gene06395.t1 AT4G28530 28.571 175 89 4 6 157 9 170 2.56e-17 79.3
MS.gene06395.t1 AT1G26870 30.247 162 100 4 7 158 16 174 3.53e-17 79.3
MS.gene06395.t1 AT5G22380 31.361 169 95 5 1 157 1 160 5.10e-17 77.0
MS.gene06395.t1 AT1G62700 29.167 168 96 6 1 158 1 155 6.75e-17 78.6
MS.gene06395.t1 AT1G62700 29.167 168 96 6 1 158 1 155 6.75e-17 78.6
MS.gene06395.t1 AT5G39820 28.177 181 109 6 9 169 22 201 1.35e-16 77.0
MS.gene06395.t1 AT1G71930 27.647 170 98 7 1 158 1 157 1.46e-16 77.0
MS.gene06395.t1 AT1G71930 27.647 170 98 7 1 158 1 157 1.46e-16 77.0
MS.gene06395.t1 AT1G54330 29.143 175 97 4 5 168 4 162 1.92e-16 76.3
MS.gene06395.t1 AT3G10480 32.759 174 97 6 7 172 27 188 2.05e-16 77.0
MS.gene06395.t1 AT3G12910 26.036 169 110 6 3 158 18 184 6.90e-16 74.7
MS.gene06395.t1 AT5G66300 27.778 162 95 6 7 158 12 161 7.89e-16 74.7
MS.gene06395.t1 AT1G32870 36.220 127 74 2 7 127 10 135 1.23e-15 75.1
MS.gene06395.t1 AT1G32870 36.220 127 74 2 7 127 10 135 1.27e-15 75.1
MS.gene06395.t1 AT5G04395 33.103 145 76 4 7 130 28 172 1.28e-15 72.0
MS.gene06395.t1 AT1G32870 36.220 127 74 2 7 127 46 171 1.71e-15 74.7
MS.gene06395.t1 AT3G49530 28.571 161 96 5 7 158 13 163 2.00e-15 74.3
MS.gene06395.t1 AT3G49530 28.571 161 96 5 7 158 13 163 3.17e-15 73.9
MS.gene06395.t1 AT3G10480 32.571 175 97 7 7 172 27 189 4.10e-15 73.6
MS.gene06395.t1 AT1G34190 32.283 127 77 2 9 127 18 143 7.54e-15 72.8
MS.gene06395.t1 AT1G02230 32.090 134 76 4 8 129 4 134 1.02e-14 72.0
MS.gene06395.t1 AT3G10490 32.000 175 98 7 7 172 27 189 1.10e-14 70.5
MS.gene06395.t1 AT5G14000 32.934 167 79 7 5 158 13 159 1.25e-14 70.1
MS.gene06395.t1 AT5G64060 28.235 170 103 4 7 168 6 164 5.37e-14 70.1
MS.gene06395.t1 AT3G10490 32.000 175 98 7 7 172 27 189 6.77e-14 70.1
MS.gene06395.t1 AT2G17040 32.143 140 83 4 1 129 1 139 2.57e-13 67.4
MS.gene06395.t1 AT5G14000 33.099 142 80 5 5 137 13 148 2.63e-13 65.5
MS.gene06395.t1 AT1G34180 31.496 127 78 2 9 127 18 143 6.14e-13 67.0
MS.gene06395.t1 AT1G34180 31.496 127 78 2 9 127 18 143 6.47e-13 67.0
MS.gene06395.t1 AT3G44350 28.395 162 91 6 10 157 8 158 7.70e-13 65.5
MS.gene06395.t1 AT5G09330 27.879 165 99 5 5 160 4 157 3.49e-12 65.1
MS.gene06395.t1 AT5G09330 27.879 165 99 5 5 160 4 157 3.49e-12 65.1
MS.gene06395.t1 AT5G09330 27.879 165 99 5 5 160 4 157 3.49e-12 65.1
MS.gene06395.t1 AT5G09330 27.879 165 99 5 5 160 4 157 3.49e-12 65.1
MS.gene06395.t1 AT5G09330 27.879 165 99 5 5 160 4 157 3.49e-12 65.1
MS.gene06395.t1 AT3G44350 29.412 136 79 4 10 131 8 140 5.16e-12 62.0
MS.gene06395.t1 AT5G61430 36.111 108 50 6 60 158 5 102 2.46e-11 61.6

Find 46 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCTTCATTCCCACTTTC+TGG 0.138453 3.2:+80470471 None:intergenic
AATAAATCCAGTACCGAAAA+TGG 0.244913 3.2:-80470542 MS.gene06395:CDS
ATTCGCCAATGTGGAATAAT+AGG 0.293651 3.2:+80470447 None:intergenic
CTTGTTCCTTGGGAATTAAA+TGG 0.298684 3.2:-80470989 MS.gene06395:intron
CCAGTACCGAAAATGGATAT+TGG 0.313420 3.2:-80470535 MS.gene06395:CDS
ATCTGTCTCTACTTGTTCCT+TGG 0.327451 3.2:-80471000 MS.gene06395:CDS
AATGGATATTGGGAGGAAAT+TGG 0.352799 3.2:-80470524 MS.gene06395:CDS
TCTGTCTCTACTTGTTCCTT+GGG 0.353376 3.2:-80470999 MS.gene06395:CDS
TGGTCAGTGCCTGAAAATAT+TGG 0.355149 3.2:+80470491 None:intergenic
GAATATCATATTTGCTCCTC+TGG 0.364606 3.2:-80470389 MS.gene06395:CDS
TCTAGATCAGGGATGAAGTT+TGG 0.373168 3.2:+80471022 None:intergenic
TGGCATGACAGGGTGAAAAT+TGG 0.385812 3.2:-80470197 MS.gene06395:intron
GATATTGTCAAGCTTCCTCC+TGG 0.398901 3.2:-80471121 MS.gene06395:CDS
CAGTACCGAAAATGGATATT+GGG 0.414415 3.2:-80470534 MS.gene06395:CDS
CAAAGTACTGTTTCATATCA+TGG 0.427460 3.2:-80470359 MS.gene06395:CDS
CACAAAGTTCTGAAACAAGT+TGG 0.433370 3.2:-80470421 MS.gene06395:CDS
AGTAGAGACAGATCTAGATC+AGG 0.454456 3.2:+80471010 None:intergenic
AGGATAAGTTCTTCATCTGT+TGG 0.457202 3.2:+80471085 None:intergenic
ACAGTGGAACTGATCTTTCA+TGG 0.461699 3.2:-80470086 MS.gene06395:CDS
AGGGTGAAAATTGGAGCGAA+TGG 0.500867 3.2:-80470188 MS.gene06395:CDS
GGGTGAAAATTGGAGCGAAT+GGG 0.512033 3.2:-80470187 MS.gene06395:CDS
ACAAAGTTCTGAAACAAGTT+GGG 0.512054 3.2:-80470420 MS.gene06395:CDS
TTCATATCATGGTGATAAAG+TGG 0.528044 3.2:-80470348 MS.gene06395:intron
TTCAGGCACTGACCAGAAAG+TGG 0.528880 3.2:-80470483 MS.gene06395:CDS
ATATTGTCAAGCTTCCTCCT+GGG 0.550955 3.2:-80471120 MS.gene06395:CDS
GGAGTACCATTTAATTCCCA+AGG 0.563304 3.2:+80470983 None:intergenic
CCTTAGAATAGAGAAAATGA+AGG 0.569373 3.2:+80471065 None:intergenic
CTATTTCTTCGCCAAAGTGA+AGG 0.570044 3.2:-80470567 MS.gene06395:CDS
TAGTGATTTACACCTTCTTG+AGG 0.571144 3.2:+80470126 None:intergenic
GTAGAGACAGATCTAGATCA+GGG 0.575051 3.2:+80471011 None:intergenic
AAGAACATGTATCCTCAAGA+AGG 0.582925 3.2:-80470138 MS.gene06395:CDS
TCAGGCACTGACCAGAAAGT+GGG 0.598459 3.2:-80470482 MS.gene06395:CDS
TACCGAAAATGGATATTGGG+AGG 0.624892 3.2:-80470531 MS.gene06395:CDS
TTTGGAGTTGGATGATGACA+TGG 0.636188 3.2:-80470043 MS.gene06395:CDS
TTTGTGCTAATTCGCCAATG+TGG 0.638216 3.2:+80470438 None:intergenic
ACAGATAAGGCATTGTGCGG+TGG 0.648627 3.2:-80470602 MS.gene06395:intron
CATACATAACTACTGAGGCA+TGG 0.664163 3.2:+80470011 None:intergenic
GAGTTGGATGATGACATGGA+GGG 0.672071 3.2:-80470039 MS.gene06395:CDS
AAGTACCTATTATTCCACAT+TGG 0.679443 3.2:-80470452 MS.gene06395:CDS
GTTGGAGAGAAGACAAACCC+AGG 0.681756 3.2:+80471103 None:intergenic
TAATGCATACATAACTACTG+AGG 0.692062 3.2:+80470006 None:intergenic
TGTACAGATAAGGCATTGTG+CGG 0.708392 3.2:-80470605 MS.gene06395:intron
GGAGAGAAGACAAACCCAGG+AGG 0.709023 3.2:+80471106 None:intergenic
AGTACTTTGATCAAATCCAG+AGG 0.713491 3.2:+80470373 None:intergenic
GGAGTTGGATGATGACATGG+AGG 0.713793 3.2:-80470040 MS.gene06395:CDS
AACAGCGATGATGATGACAG+TGG 0.723595 3.2:-80470102 MS.gene06395:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAAAATATTGAAGAAA+TGG + chr3.2:80470188-80470207 None:intergenic 10.0%
!! CTATAGTTTAATTAATTGAA+TGG - chr3.2:80470916-80470935 MS.gene06395:intron 15.0%
!! AAGAAAAAAAAAAAAAGACC+AGG - chr3.2:80470307-80470326 MS.gene06395:intron 20.0%
!! GATACATAACATAAGATATA+TGG + chr3.2:80470387-80470406 None:intergenic 20.0%
!! TATAGATATATAGCTAACTA+GGG + chr3.2:80470901-80470920 None:intergenic 20.0%
!! TTATTTAACATGTACAGATA+AGG - chr3.2:80470532-80470551 MS.gene06395:CDS 20.0%
! CTATAGATATATAGCTAACT+AGG + chr3.2:80470902-80470921 None:intergenic 25.0%
! TCATCGAAAGTAGTAAAATT+TGG + chr3.2:80470236-80470255 None:intergenic 25.0%
AAGTACCTATTATTCCACAT+TGG - chr3.2:80470695-80470714 MS.gene06395:intron 30.0%
CCTTAGAATAGAGAAAATGA+AGG + chr3.2:80470085-80470104 None:intergenic 30.0%
TTCATATCATGGTGATAAAG+TGG - chr3.2:80470799-80470818 MS.gene06395:intron 30.0%
TTCATCGAATATTCATGCAT+TGG + chr3.2:80470492-80470511 None:intergenic 30.0%
! AATAAATCCAGTACCGAAAA+TGG - chr3.2:80470605-80470624 MS.gene06395:intron 30.0%
! CCTTCATTTTCTCTATTCTA+AGG - chr3.2:80470082-80470101 MS.gene06395:CDS 30.0%
! GTTACAGTACCAATATTTTC+AGG - chr3.2:80470647-80470666 MS.gene06395:intron 30.0%
! TGTAGTATAAGTTCTAATCC+TGG + chr3.2:80470328-80470347 None:intergenic 30.0%
!! ACAAAGTTCTGAAACAAGTT+GGG - chr3.2:80470727-80470746 MS.gene06395:intron 30.0%
!! CAAAGTACTGTTTCATATCA+TGG - chr3.2:80470788-80470807 MS.gene06395:intron 30.0%
!! GGATTTATTTTCCTTCACTT+TGG + chr3.2:80470594-80470613 None:intergenic 30.0%
AAGAACATGTATCCTCAAGA+AGG - chr3.2:80471009-80471028 MS.gene06395:CDS 35.0%
AATGGATATTGGGAGGAAAT+TGG - chr3.2:80470623-80470642 MS.gene06395:intron 35.0%
ACTCCAAAGACAGAAAAACT+TGG + chr3.2:80471092-80471111 None:intergenic 35.0%
AGGATAAGTTCTTCATCTGT+TGG + chr3.2:80470065-80470084 None:intergenic 35.0%
AGTACTTTGATCAAATCCAG+AGG + chr3.2:80470777-80470796 None:intergenic 35.0%
ATTCGCCAATGTGGAATAAT+AGG + chr3.2:80470703-80470722 None:intergenic 35.0%
CTTGTTCCTTGGGAATTAAA+TGG - chr3.2:80470158-80470177 MS.gene06395:CDS 35.0%
GAATATCATATTTGCTCCTC+TGG - chr3.2:80470758-80470777 MS.gene06395:intron 35.0%
TAGTGATTTACACCTTCTTG+AGG + chr3.2:80471024-80471043 None:intergenic 35.0%
! AGACCAAGTTTTTCTGTCTT+TGG - chr3.2:80471086-80471105 MS.gene06395:CDS 35.0%
! CAGTACCGAAAATGGATATT+GGG - chr3.2:80470613-80470632 MS.gene06395:intron 35.0%
! TAGCTGTGTGTATGTGATTT+TGG + chr3.2:80470429-80470448 None:intergenic 35.0%
! TTCCTCCCAATATCCATTTT+CGG + chr3.2:80470621-80470640 None:intergenic 35.0%
!! CACAAAGTTCTGAAACAAGT+TGG - chr3.2:80470726-80470745 MS.gene06395:intron 35.0%
!!! AGTTTTTCTGTCTTTGGAGT+TGG - chr3.2:80471092-80471111 MS.gene06395:CDS 35.0%
ACAGTGGAACTGATCTTTCA+TGG - chr3.2:80471061-80471080 MS.gene06395:CDS 40.0%
ACTTCTTCATTCCCACTTTC+TGG + chr3.2:80470679-80470698 None:intergenic 40.0%
AGTAGAGACAGATCTAGATC+AGG + chr3.2:80470140-80470159 None:intergenic 40.0%
ATATTGTCAAGCTTCCTCCT+GGG - chr3.2:80470027-80470046 MS.gene06395:CDS 40.0%
ATCTGTCTCTACTTGTTCCT+TGG - chr3.2:80470147-80470166 MS.gene06395:CDS 40.0%
ATTGAATGGTGCATCATGCA+TGG - chr3.2:80470930-80470949 MS.gene06395:intron 40.0%
CATGCATTGGACATGTGTAT+TGG + chr3.2:80470479-80470498 None:intergenic 40.0%
GTAGAGACAGATCTAGATCA+GGG + chr3.2:80470139-80470158 None:intergenic 40.0%
TACCGAAAATGGATATTGGG+AGG - chr3.2:80470616-80470635 MS.gene06395:intron 40.0%
TCTAGATCAGGGATGAAGTT+TGG + chr3.2:80470128-80470147 None:intergenic 40.0%
TCTGTCTCTACTTGTTCCTT+GGG - chr3.2:80470148-80470167 MS.gene06395:CDS 40.0%
TGGTCAGTGCCTGAAAATAT+TGG + chr3.2:80470659-80470678 None:intergenic 40.0%
TTTGTGCTAATTCGCCAATG+TGG + chr3.2:80470712-80470731 None:intergenic 40.0%
! CCAATATCCATTTTCGGTAC+TGG + chr3.2:80470615-80470634 None:intergenic 40.0%
! CCAGTACCGAAAATGGATAT+TGG - chr3.2:80470612-80470631 MS.gene06395:intron 40.0%
! CTATTTCTTCGCCAAAGTGA+AGG - chr3.2:80470580-80470599 MS.gene06395:CDS 40.0%
! GGAGTACCATTTAATTCCCA+AGG + chr3.2:80470167-80470186 None:intergenic 40.0%
! TTTGGAGTTGGATGATGACA+TGG - chr3.2:80471104-80471123 MS.gene06395:CDS 40.0%
!! TGTACAGATAAGGCATTGTG+CGG - chr3.2:80470542-80470561 MS.gene06395:CDS 40.0%
AGGGTGAAAATTGGAGCGAA+TGG - chr3.2:80470959-80470978 MS.gene06395:intron 45.0%
GAGTTGGATGATGACATGGA+GGG - chr3.2:80471108-80471127 MS.gene06395:CDS 45.0%
GATATTGTCAAGCTTCCTCC+TGG - chr3.2:80470026-80470045 MS.gene06395:CDS 45.0%
GGGTGAAAATTGGAGCGAAT+GGG - chr3.2:80470960-80470979 MS.gene06395:intron 45.0%
TGGCATGACAGGGTGAAAAT+TGG - chr3.2:80470950-80470969 MS.gene06395:intron 45.0%
! AACAGCGATGATGATGACAG+TGG - chr3.2:80471045-80471064 MS.gene06395:CDS 45.0%
!! AATTTAATATATTGAATATA+TGG - chr3.2:80470360-80470379 MS.gene06395:CDS 5.0%
GCATCATGCATGGCATGACA+GGG - chr3.2:80470940-80470959 MS.gene06395:intron 50.0%
GGAGTTGGATGATGACATGG+AGG - chr3.2:80471107-80471126 MS.gene06395:CDS 50.0%
GTTGGAGAGAAGACAAACCC+AGG + chr3.2:80470047-80470066 None:intergenic 50.0%
TGCATCATGCATGGCATGAC+AGG - chr3.2:80470939-80470958 MS.gene06395:intron 50.0%
!! ACAGATAAGGCATTGTGCGG+TGG - chr3.2:80470545-80470564 MS.gene06395:CDS 50.0%
!! TCAGGCACTGACCAGAAAGT+GGG - chr3.2:80470665-80470684 MS.gene06395:intron 50.0%
!! TTCAGGCACTGACCAGAAAG+TGG - chr3.2:80470664-80470683 MS.gene06395:intron 50.0%
GGAGAGAAGACAAACCCAGG+AGG + chr3.2:80470044-80470063 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 80470020 80471149 80470020 ID=MS.gene06395
chr3.2 mRNA 80470020 80471149 80470020 ID=MS.gene06395.t1;Parent=MS.gene06395
chr3.2 exon 80470990 80471149 80470990 ID=MS.gene06395.t1.exon1;Parent=MS.gene06395.t1
chr3.2 CDS 80470990 80471149 80470990 ID=cds.MS.gene06395.t1;Parent=MS.gene06395.t1
chr3.2 exon 80470349 80470620 80470349 ID=MS.gene06395.t1.exon2;Parent=MS.gene06395.t1
chr3.2 CDS 80470349 80470620 80470349 ID=cds.MS.gene06395.t1;Parent=MS.gene06395.t1
chr3.2 exon 80470020 80470208 80470020 ID=MS.gene06395.t1.exon3;Parent=MS.gene06395.t1
chr3.2 CDS 80470020 80470208 80470020 ID=cds.MS.gene06395.t1;Parent=MS.gene06395.t1
Gene Sequence

>MS.gene06395

ATGGGAGATATTGTCAAGCTTCCTCCTGGGTTTGTCTTCTCTCCAACAGATGAAGAACTTATCCTTCATTTTCTCTATTCTAAGGCTTCTCTTATTCAATCTCATCCAAACTTCATCCCTGATCTAGATCTGTCTCTACTTGTTCCTTGGGAATTAAATGGTACTCCATTTCTTCAATATTTTTAATTGTCGTTTTAAGATTATTTATACGTACCAAATTTTACTACTTTCGATGAGATTGATTATATTTTTCTAGATTATTTTTATTTATTCTTTATTTCAAAAGAAAGAAAAAAAAAAAAAGACCAGGATTAGAACTTATACTACATTTTCACTAAAAAATTTAATATATTGAATATATGGACCATATATCTTATGTTATGTATCATTCATTTGATTCAATTATCCAAAATCACATACACACAGCTAGAGAAAGATTAAGCGACATATAATTAACCAATACACATGTCCAATGCATGAATATTCGATGAATGAACTTCATATTTAAATTCTTATTTAACATGTACAGATAAGGCATTGTGCGGTGGAAATGATGAATACTATTTCTTCGCCAAAGTGAAGGAAAATAAATCCAGTACCGAAAATGGATATTGGGAGGAAATTGGTGTTACAGTACCAATATTTTCAGGCACTGACCAGAAAGTGGGAATGAAGAAGTACCTATTATTCCACATTGGCGAATTAGCACAAAGTTCTGAAACAAGTTGGGTAATGCAAGAATATCATATTTGCTCCTCTGGATTTGATCAAAGTACTGTTTCATATCATGGTGATAAAGTGGTAAGTGACATTATTTATTTTGACAATAAGCTAATAAAATGATGCATTGATATCTAGCTAATTAAAAATTATTTTCTCCCTAGTTAGCTATATATCTATAGTTTAATTAATTGAATGGTGCATCATGCATGGCATGACAGGGTGAAAATTGGAGCGAATGGGTTTTATGCAGAGTCTATGAGATTGATAAGAACATGTATCCTCAAGAAGGTGTAAATCACTACAACAGCGATGATGATGACAGTGGAACTGATCTTTCATGGCAAGACCAAGTTTTTCTGTCTTTGGAGTTGGATGATGACATGGAGGGAATAACCATGCCTCAGTAG
Protein sequence

>MS.gene06395.t1

MGDIVKLPPGFVFSPTDEELILHFLYSKASLIQSHPNFIPDLDLSLLVPWELNDKALCGGNDEYYFFAKVKENKSSTENGYWEEIGVTVPIFSGTDQKVGMKKYLLFHIGELAQSSETSWVMQEYHICSSGFDQSTVSYHGDKVGENWSEWVLCRVYEIDKNMYPQEGVNHYNSDDDDSGTDLSWQDQVFLSLELDDDMEGITMPQ