AlfalfaGEDB | MS.gene064031

M. sativa cultivar XinJiangDaYe / MS.gene064031

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064031.t1	XP_003620872.2	69.8	159	32	2	45	187	708	866	1.10E-49	206.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064031.t1	A0A072UCR4	69.8	159	32	2	45	187	643	801	8.0e-50	206.5

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054291	MS.gene064031	-0.803966	2.71E-49	-1.69E-46
MS.gene056174	MS.gene064031	0.814583	1.43E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064031.t1	MTR_6g091690	69.811	159	32	2	45	187	643	801	6.44e-65	214
MS.gene064031.t1	MTR_6g091690	69.811	159	32	2	45	187	708	866	1.19e-64	214
MS.gene064031.t1	MTR_6g091710	64.286	98	27	2	26	121	50	141	7.71e-33	115

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 32 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGATTTACAACACAGTC+AGG	0.467267	6.1:+79324362	None:intergenic
AAATATACATCCGAAAGCCG+AGG	0.671474	6.1:-79323499	MS.gene064031:CDS
AACGATTTACAACACAGTCA+GGG	0.666758	6.1:+79324363	None:intergenic
AATACTGAAACCAGATCTTC+AGG	0.417052	6.1:-79323925	MS.gene064031:intron
ACTAATGAACCATCTTCAAC+AGG	0.475523	6.1:-79323775	MS.gene064031:CDS
AGATGATGTTTCAACTTCAG+TGG	0.545104	6.1:-79323596	MS.gene064031:CDS
AGCCGACATTTCTATTTCCT+CGG	0.444211	6.1:+79323482	None:intergenic
AGCCGAGGAAATAGAAATGT+CGG	0.590279	6.1:-79323484	MS.gene064031:CDS
AGCTACAGAACGTTGAAGGG+TGG	0.614968	6.1:-79323667	MS.gene064031:CDS
ATCTTGCGTAGAAAGTGGCA+TGG	0.614247	6.1:-79323695	MS.gene064031:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAAAAATACTGTCTAGCTG+AGG	-	chr6.1:79323492-79323511	MS.gene064031:CDS	30.0%
ATGATTATCTCCAAATGACT+TGG	+	chr6.1:79323539-79323558	None:intergenic	30.0%
TGATTATCTCCAAATGACTT+GGG	+	chr6.1:79323538-79323557	None:intergenic	30.0%
TTGTGTTGTAGATGTGAAAT+AGG	+	chr6.1:79323759-79323778	None:intergenic	30.0%
TTTGTATATGCATCATGCAT+TGG	+	chr6.1:79323788-79323807	None:intergenic	30.0%
AAACGATTTACAACACAGTC+AGG	+	chr6.1:79323447-79323466	None:intergenic	35.0%
AACGATTTACAACACAGTCA+GGG	+	chr6.1:79323446-79323465	None:intergenic	35.0%
AATACTGAAACCAGATCTTC+AGG	-	chr6.1:79323881-79323900	MS.gene064031:intron	35.0%
ACTAATGAACCATCTTCAAC+AGG	-	chr6.1:79324031-79324050	MS.gene064031:intron	35.0%
AGATGATGTTTCAACTTCAG+TGG	-	chr6.1:79324210-79324229	MS.gene064031:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	79323396	79324432	79323396	ID=MS.gene064031
chr6.1	mRNA	79323396	79324432	79323396	ID=MS.gene064031.t1;Parent=MS.gene064031
chr6.1	exon	79324366	79324432	79324366	ID=MS.gene064031.t1.exon1;Parent=MS.gene064031.t1
chr6.1	CDS	79324366	79324432	79324366	ID=cds.MS.gene064031.t1;Parent=MS.gene064031.t1
chr6.1	exon	79323926	79323978	79323926	ID=MS.gene064031.t1.exon2;Parent=MS.gene064031.t1
chr6.1	CDS	79323926	79323978	79323926	ID=cds.MS.gene064031.t1;Parent=MS.gene064031.t1
chr6.1	exon	79323396	79323839	79323396	ID=MS.gene064031.t1.exon3;Parent=MS.gene064031.t1
chr6.1	CDS	79323396	79323839	79323396	ID=cds.MS.gene064031.t1;Parent=MS.gene064031.t1

Gene Sequence

>MS.gene064031

ATGTCAAAGCAAGGAAAAGAATTTACCTCAGCAGCACTTGACCTACTCCCTGACTGTGTTGTAAATCGTTTGGCAAGTTTCTCTGGTCCAACGAAGATAAAAATACTGTCTAGCTGAGGAGCATAATATCAAATGGGAACCCAAGTCATTTGGAGATAATCATACAAAGGCTTCTCAAGACTTGCTGGTAGGATTTGTTTATTCCCTAGTTTATTTTGATAAAAAAAAGTTGTTACTATAGTTTATCATTTTGACACTATTTATATGTTAAGGTTGGGCCAGGTACTCTTGAAAATGCCTGCTTATGAGGAACCTTTTCAAGTTCATGTTCTACCACCTGTTCATGATGAAAAGCGTCCTCCTATTTCACATCTACAACACAACCTAAACCAATGCATGATGCATATACAAATTCGTTTGAGCAGAGTCTAAATACTGCTGCTTGAAAGGATAGTGCCAATAACTCTACAACATCAGGCATGCCTAATACTGAAACCAGATCTTCAGGTACTTTTTTAAAGAAAGTCATTACAATTGAATATCTAAGCTTCTTTTTTGTTCTTTACCTGAATTATTTGAGATTCTTTTCGCAGGAGGTGGAAGTCAAAAAAAATTATGGTTACGATGACTTGCCAACTAATGAACCATCTTCAACAGGAAGAAACTCGACATTGCAGTCACCTAATAATTTTGAGGATACTGAAACTTTGGAGAAATCTTGCGTAGAAAGTGGCATGGAATTAAGCTACAGAACGTTGAAGGGTGGTTTTCGCAATAAAGGTTGTACACAACCACCTTATATTAAGAATACATCAGATGATGTTTCAACTTCAGTGGGAAACATTTCAATCCAGAGTGAAAGATTGTGTGCAGTGAGGATTTCAACCAGTTTTGATGCTCCTGTTCAGGACAAATATACATCCGAAAGCCGAGGAAATAGAAATGTCGGCTTGAAAGCACACAACAAATCTTCTGATTCTGATAGCTACGATCTGGTTGCAGACTCACACCATGAACTTCACATTCAGAATGAGTAG

Protein sequence

>MS.gene064031.t1

MSKQGKEFTSAALDLLPDCVVNLPITLQHQACLILKPDLQEVEVKKNYGYDDLPTNEPSSTGRNSTLQSPNNFEDTETLEKSCVESGMELSYRTLKGGFRNKGCTQPPYIKNTSDDVSTSVGNISIQSERLCAVRISTSFDAPVQDKYTSESRGNRNVGLKAHNKSSDSDSYDLVADSHHELHIQNE