Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064040.t1 | XP_003620882.1 | 91.7 | 278 | 22 | 1 | 1 | 278 | 1 | 277 | 9.30E-136 | 493 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064040.t1 | G7KJ82 | 91.7 | 278 | 22 | 1 | 1 | 278 | 1 | 277 | 6.7e-136 | 493.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050885 | MS.gene064040 | 0.811264 | 7.64E-51 | -1.69E-46 |
| MS.gene056403 | MS.gene064040 | 0.86342 | 2.52E-64 | -1.69E-46 |
| MS.gene056605 | MS.gene064040 | 0.859901 | 3.00E-63 | -1.69E-46 |
| MS.gene05689 | MS.gene064040 | 0.800811 | 1.21E-48 | -1.69E-46 |
| MS.gene060830 | MS.gene064040 | 0.836832 | 7.42E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064040.t1 | MTR_6g091790 | 91.367 | 278 | 23 | 1 | 1 | 278 | 1 | 277 | 1.32e-179 | 500 |
| MS.gene064040.t1 | MTR_6g091790 | 91.367 | 278 | 23 | 1 | 1 | 278 | 1 | 277 | 8.62e-178 | 501 |
| MS.gene064040.t1 | MTR_6g092160 | 90.647 | 278 | 24 | 1 | 1 | 276 | 1 | 278 | 2.18e-167 | 466 |
| MS.gene064040.t1 | MTR_6g092160 | 91.304 | 276 | 22 | 1 | 1 | 274 | 1 | 276 | 4.19e-167 | 465 |
| MS.gene064040.t1 | MTR_6g091900 | 80.645 | 279 | 32 | 3 | 1 | 278 | 1 | 258 | 1.01e-150 | 429 |
| MS.gene064040.t1 | MTR_6g092190 | 75.097 | 257 | 30 | 4 | 1 | 256 | 1 | 224 | 5.69e-123 | 353 |
| MS.gene064040.t1 | MTR_1g016740 | 32.014 | 278 | 165 | 6 | 14 | 278 | 14 | 280 | 5.18e-31 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064040.t1 | AT5G11780 | 30.037 | 273 | 169 | 8 | 18 | 281 | 21 | 280 | 1.01e-27 | 112 |
Find 0 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATAAGTATGTTTAATT+TGG | - | 42523:12213-12232 | None:intergenic | 10.0% |
| !! | ACATTATCTCAAATTATTAT+CGG | - | 42523:11970-11989 | None:intergenic | 15.0% |
| !! | ACTAATATCCATAAAAATAA+TGG | - | 42523:12453-12472 | None:intergenic | 15.0% |
| !! | AATTATTTGTGAAGGTAAAT+TGG | - | 42523:12896-12915 | None:intergenic | 20.0% |
| !!! | TTAGTTATTGACTTATTGTA+TGG | + | 42523:12745-12764 | MS.gene064040:intron | 20.0% |
| !!! | TTGTGTTTGTTTTTTGATAT+GGG | + | 42523:11467-11486 | MS.gene064040:CDS | 20.0% |
| !!! | TTTAAGGTAAAATATGTAGT+AGG | - | 42523:11903-11922 | None:intergenic | 20.0% |
| ! | ACCAGTTTAATTATTTGTGA+AGG | - | 42523:12904-12923 | None:intergenic | 25.0% |
| ! | ACCTTCACAAATAATTAAAC+TGG | + | 42523:12900-12919 | MS.gene064040:intron | 25.0% |
| ! | CAGATATGGAATAAATAACA+AGG | - | 42523:12378-12397 | None:intergenic | 25.0% |
| ! | CTATGTATCATAAAAACAAC+AGG | - | 42523:11131-11150 | None:intergenic | 25.0% |
| ! | GTTGAATGTAACTATATCTT+TGG | + | 42523:11703-11722 | MS.gene064040:intron | 25.0% |
| ! | TAGAATCATCTCTTATAGTA+AGG | + | 42523:12858-12877 | MS.gene064040:intron | 25.0% |
| ! | TGAAGCATAATTTCTGTTAA+TGG | + | 42523:12810-12829 | MS.gene064040:intron | 25.0% |
| !! | AATTTCTGAAGAGAAAGATT+TGG | + | 42523:11256-11275 | MS.gene064040:CDS | 25.0% |
| !! | ACATAGCTTAACTTTTTATC+TGG | + | 42523:12041-12060 | MS.gene064040:intron | 25.0% |
| !! | CATAGCTTAAAACTTTTATC+TGG | + | 42523:11804-11823 | MS.gene064040:intron | 25.0% |
| !! | TAAAACTTTTATCTGGATGA+TGG | + | 42523:11811-11830 | MS.gene064040:intron | 25.0% |
| !! | TCAATGTTTCATAGGTTTTA+AGG | - | 42523:11636-11655 | None:intergenic | 25.0% |
| !! | TTAACTTTTTATCTGGATGA+GGG | + | 42523:12048-12067 | MS.gene064040:intron | 25.0% |
| !! | TTAGACATTTTTGCAATACA+CGG | - | 42523:12428-12447 | None:intergenic | 25.0% |
| !! | TTTATCTTTTGTACAATCAG+AGG | + | 42523:12289-12308 | MS.gene064040:intron | 25.0% |
| !! | TTTTGCTAAAAGCTATACTT+TGG | - | 42523:12140-12159 | None:intergenic | 25.0% |
| !!! | ATCAGTGTTTCATAGTTTTA+AGG | - | 42523:11919-11938 | None:intergenic | 25.0% |
| !!! | GTCTAACACCATTATTTTTA+TGG | + | 42523:12442-12461 | MS.gene064040:intron | 25.0% |
| !!! | GTTGTGTTTGTTTTTTGATA+TGG | + | 42523:11466-11485 | MS.gene064040:CDS | 25.0% |
| !!! | TGTTTTTATGATACATAGTG+TGG | + | 42523:11133-11152 | MS.gene064040:CDS | 25.0% |
| !!! | TTTCAGAATGTGTACTTTTT+GGG | + | 42523:11551-11570 | MS.gene064040:CDS | 25.0% |
| !!! | TTTGTTTTTTGATATGGGAA+TGG | + | 42523:11472-11491 | MS.gene064040:CDS | 25.0% |
| AATGAAGGAAGTGAAATCTT+TGG | + | 42523:11193-11212 | MS.gene064040:CDS | 30.0% | |
| AATGGAATGAGAAAATTCAG+GGG | - | 42523:10907-10926 | None:intergenic | 30.0% | |
| AGGAACAGTTTGAATTGAAA+AGG | + | 42523:11159-11178 | MS.gene064040:CDS | 30.0% | |
| CAATGGAATGAGAAAATTCA+GGG | - | 42523:10908-10927 | None:intergenic | 30.0% | |
| CACATGTGTAATAATCAATC+TGG | - | 42523:12698-12717 | None:intergenic | 30.0% | |
| CAGATTGATTATTACACATG+TGG | + | 42523:12696-12715 | MS.gene064040:intron | 30.0% | |
| CCATTAAAGCTGTTAACATT+TGG | + | 42523:12549-12568 | MS.gene064040:intron | 30.0% | |
| CTTCACATTGAATGTACATT+CGG | - | 42523:11773-11792 | None:intergenic | 30.0% | |
| GGAACAGTTTGAATTGAAAA+GGG | + | 42523:11160-11179 | MS.gene064040:CDS | 30.0% | |
| GTACATTCAATGTGAAGTAA+TGG | + | 42523:11776-11795 | MS.gene064040:intron | 30.0% | |
| GTCTATTCTGCAATCAAATT+TGG | + | 42523:10984-11003 | MS.gene064040:CDS | 30.0% | |
| TACTTACTTATAAGTGCTCT+CGG | - | 42523:12979-12998 | None:intergenic | 30.0% | |
| TATGATACATAGTGTGGTTA+AGG | + | 42523:11139-11158 | MS.gene064040:CDS | 30.0% | |
| TCAATGGAATGAGAAAATTC+AGG | - | 42523:10909-10928 | None:intergenic | 30.0% | |
| TCTGTTAATGGTTTGATGTA+GGG | + | 42523:12822-12841 | MS.gene064040:intron | 30.0% | |
| TGAAGAGAAAGATTTGGATT+TGG | + | 42523:11262-11281 | MS.gene064040:CDS | 30.0% | |
| TGTAATAATCAATCTGGTCA+AGG | - | 42523:12692-12711 | None:intergenic | 30.0% | |
| TGTCAGTATCAATGTTTCAT+AGG | - | 42523:11644-11663 | None:intergenic | 30.0% | |
| TTCTGATAGAATTTCTTGCA+TGG | - | 42523:10871-10890 | None:intergenic | 30.0% | |
| TTCTGTTAATGGTTTGATGT+AGG | + | 42523:12821-12840 | MS.gene064040:intron | 30.0% | |
| TTTGATATGGGAATGGAAAA+GGG | + | 42523:11479-11498 | MS.gene064040:CDS | 30.0% | |
| TTTGTTTGATGCTGTACTAT+TGG | + | 42523:12618-12637 | MS.gene064040:intron | 30.0% | |
| ! | AGACTTTCATGTTGGTTATT+TGG | + | 42523:11409-11428 | MS.gene064040:CDS | 30.0% |
| ! | ATTCCATTCAATGCACTTTT+TGG | + | 42523:11838-11857 | MS.gene064040:intron | 30.0% |
| ! | CCAAATGTTAACAGCTTTAA+TGG | - | 42523:12552-12571 | None:intergenic | 30.0% |
| ! | CTTAACTTTTTATCTGGATG+AGG | + | 42523:12047-12066 | MS.gene064040:intron | 30.0% |
| ! | CTTTCAGAATGTGTACTTTT+TGG | + | 42523:11550-11569 | MS.gene064040:CDS | 30.0% |
| ! | CTTTGGTTGATATGATTCTT+GGG | + | 42523:11210-11229 | MS.gene064040:CDS | 30.0% |
| ! | GTGTTGTTCTTTGTTTAATG+TGG | + | 42523:12265-12284 | MS.gene064040:intron | 30.0% |
| ! | TAGACATTTTTGCAATACAC+GGG | - | 42523:12427-12446 | None:intergenic | 30.0% |
| ! | TCTTTGGTTGATATGATTCT+TGG | + | 42523:11209-11228 | MS.gene064040:CDS | 30.0% |
| ! | TTAATTTGGTCTGTCTGAAT+TGG | - | 42523:12199-12218 | None:intergenic | 30.0% |
| ! | TTTTGATATGGGAATGGAAA+AGG | + | 42523:11478-11497 | MS.gene064040:CDS | 30.0% |
| !! | GGTTTTGTCTTTGAATGATT+TGG | + | 42523:11283-11302 | MS.gene064040:CDS | 30.0% |
| !! | TTCCATTCAATGCACTTTTT+GGG | + | 42523:11839-11858 | MS.gene064040:intron | 30.0% |
| !!! | GGTGAGGTTTATTTTGTTTT+TGG | + | 42523:11572-11591 | MS.gene064040:intron | 30.0% |
| AAGGGAGAAGAAAGTAATGA+AGG | + | 42523:11178-11197 | MS.gene064040:CDS | 35.0% | |
| AGAAAATTCAGGGGATTCTT+TGG | - | 42523:10898-10917 | None:intergenic | 35.0% | |
| ATACCAACAATTCACACCAA+AGG | - | 42523:12020-12039 | None:intergenic | 35.0% | |
| ATCAAAAGATGGGTCAAGTT+TGG | - | 42523:10955-10974 | None:intergenic | 35.0% | |
| ATTATCGGTCTTAATACGTG+TGG | - | 42523:11955-11974 | None:intergenic | 35.0% | |
| CAGTTCATGTAATGCATCAA+TGG | - | 42523:10925-10944 | None:intergenic | 35.0% | |
| GAAAATTCAGGGGATTCTTT+GGG | - | 42523:10897-10916 | None:intergenic | 35.0% | |
| GGTTGCAGAAAATCTCTTAA+AGG | + | 42523:11005-11024 | MS.gene064040:CDS | 35.0% | |
| TACAATCAGAGGTTCTTATG+AGG | + | 42523:12300-12319 | MS.gene064040:intron | 35.0% | |
| TGTGGTATAGACTTTCATGT+TGG | + | 42523:11401-11420 | MS.gene064040:CDS | 35.0% | |
| ! | AAACTGGTTTCAAACCAGAA+TGG | + | 42523:12916-12935 | MS.gene064040:intron | 35.0% |
| ! | ACAAATTTGGTTCCTCATGT+TGG | + | 42523:10816-10835 | MS.gene064040:CDS | 35.0% |
| ! | TAGCTTTTAGCAAAATCACG+AGG | + | 42523:12145-12164 | MS.gene064040:intron | 35.0% |
| ! | TTTCATGTTGGTTATTTGGC+TGG | + | 42523:11413-11432 | MS.gene064040:CDS | 35.0% |
| !! | TGAGTTTGTTAAAATCACCG+TGG | + | 42523:12100-12119 | MS.gene064040:intron | 35.0% |
| ACCCACATCACATCAAAAGA+TGG | - | 42523:10966-10985 | None:intergenic | 40.0% | |
| ATTTGGTCTGTCTGAATTGG+CGG | - | 42523:12196-12215 | None:intergenic | 40.0% | |
| CAAACATCACTACTCACAAG+AGG | - | 42523:11371-11390 | None:intergenic | 40.0% | |
| CCCACATCACATCAAAAGAT+GGG | - | 42523:10965-10984 | None:intergenic | 40.0% | |
| CTGTGATTACACTGCAGATA+TGG | - | 42523:12392-12411 | None:intergenic | 40.0% | |
| CTTGTGAGTAGTGATGTTTG+CGG | + | 42523:11371-11390 | MS.gene064040:CDS | 40.0% | |
| GGATGAATTCACTGTATGCT+TGG | + | 42523:12069-12088 | MS.gene064040:intron | 40.0% | |
| GTTTGATGCTGTACTATTGG+TGG | + | 42523:12621-12640 | MS.gene064040:intron | 40.0% | |
| TAATCAATCTGGTCAAGGCA+AGG | - | 42523:12687-12706 | None:intergenic | 40.0% | |
| TATCTTTGGATGTGTCGTGT+CGG | + | 42523:11717-11736 | MS.gene064040:intron | 40.0% | |
| TCACTGTATGCTTGGATTGA+CGG | + | 42523:12077-12096 | MS.gene064040:intron | 40.0% | |
| TGGAAATGTGCCTTAAGAGT+TGG | + | 42523:11507-11526 | MS.gene064040:CDS | 40.0% | |
| TTCAAACCAGAATGGTATCG+AGG | + | 42523:12924-12943 | MS.gene064040:intron | 40.0% | |
| TTTGCCTCTGAACTCAATAC+TGG | + | 42523:12340-12359 | MS.gene064040:CDS | 40.0% | |
| ! | AATGGTATCGAGGAAAATGC+CGG | + | 42523:12934-12953 | MS.gene064040:CDS | 40.0% |
| ! | ACCCATCTTTTGATGTGATG+TGG | + | 42523:10961-10980 | MS.gene064040:CDS | 40.0% |
| ! | ACGATGATGATGCAAATGAG+GGG | + | 42523:11330-11349 | MS.gene064040:CDS | 40.0% |
| ! | ATGCCTTTGGTGTGAATTGT+TGG | + | 42523:12014-12033 | MS.gene064040:intron | 40.0% |
| ! | ATGTGGCTGTAGAGTATCTT+AGG | + | 42523:12713-12732 | MS.gene064040:intron | 40.0% |
| ! | ATTCACACCAAAGGCATATC+CGG | - | 42523:12011-12030 | None:intergenic | 40.0% |
| ! | CACCCAAAAAGTGCATTGAA+TGG | - | 42523:11844-11863 | None:intergenic | 40.0% |
| ! | CATTTTCCTCGATACCATTC+TGG | - | 42523:12933-12952 | None:intergenic | 40.0% |
| ! | CCCATCTTTTGATGTGATGT+GGG | + | 42523:10962-10981 | MS.gene064040:CDS | 40.0% |
| ! | CTAACCAGTATTGAGTTCAG+AGG | - | 42523:12347-12366 | None:intergenic | 40.0% |
| ! | GAATGATTTGGCTCGTCTTT+GGG | + | 42523:11295-11314 | MS.gene064040:CDS | 40.0% |
| ! | GACGATGATGATGCAAATGA+GGG | + | 42523:11329-11348 | MS.gene064040:CDS | 40.0% |
| ! | GGCAAGGCTTTTTATGTTCT+TGG | - | 42523:12671-12690 | None:intergenic | 40.0% |
| ! | GTGTAGTTTTCACCAACATG+AGG | - | 42523:10831-10850 | None:intergenic | 40.0% |
| ! | TGAATGATTTGGCTCGTCTT+TGG | + | 42523:11294-11313 | MS.gene064040:CDS | 40.0% |
| !! | CATGGTGTTTTTCAGCTTCA+GGG | - | 42523:10853-10872 | None:intergenic | 40.0% |
| !!! | GAATGTGTACTTTTTGGGTG+AGG | + | 42523:11556-11575 | MS.gene064040:intron | 40.0% |
| !!! | TTCAATGCACTTTTTGGGTG+AGG | + | 42523:11844-11863 | MS.gene064040:intron | 40.0% |
| ACTTATAAGTGCTCTCGGTG+AGG | - | 42523:12974-12993 | None:intergenic | 45.0% | |
| AGCCAACACTCCAACTCTTA+AGG | - | 42523:11520-11539 | None:intergenic | 45.0% | |
| GATGTTTGCGGTTGGATTTG+TGG | + | 42523:11383-11402 | MS.gene064040:CDS | 45.0% | |
| GCTTGTTCAGCTTCTGTTAG+TGG | + | 42523:11080-11099 | MS.gene064040:CDS | 45.0% | |
| GGGAATGGAAAAGGGTGAAT+TGG | + | 42523:11487-11506 | MS.gene064040:CDS | 45.0% | |
| GGTCTTAATACGTGTGGTGT+AGG | - | 42523:11949-11968 | None:intergenic | 45.0% | |
| GTCTGAATTGGCGGTGAAAT+TGG | - | 42523:12187-12206 | None:intergenic | 45.0% | |
| TATCGATCCATGACGTGTAC+CGG | + | 42523:11989-12008 | MS.gene064040:intron | 45.0% | |
| TGAGTAGTGATGTTTGCGGT+TGG | + | 42523:11375-11394 | MS.gene064040:CDS | 45.0% | |
| TGCCTTAAGAGTTGGAGTGT+TGG | + | 42523:11515-11534 | MS.gene064040:CDS | 45.0% | |
| TGTGCGTCATTGACACACAT+CGG | + | 42523:11743-11762 | MS.gene064040:intron | 45.0% | |
| ! | CGACGATGATGATGCAAATG+AGG | + | 42523:11328-11347 | MS.gene064040:CDS | 45.0% |
| ! | TGGAGCATCACAAAACACCA+CGG | - | 42523:12120-12139 | None:intergenic | 45.0% |
| !! | GCATGGTGTTTTTCAGCTTC+AGG | - | 42523:10854-10873 | None:intergenic | 45.0% |
| !! | TTTGGCTGGTGCTGTTATGA+TGG | + | 42523:11427-11446 | MS.gene064040:CDS | 45.0% |
| ACGTGTACCGGATATGCCTT+TGG | + | 42523:12001-12020 | MS.gene064040:intron | 50.0% | |
| GAAAATGCCGGCAAGGCAAA+TGG | + | 42523:12946-12965 | MS.gene064040:CDS | 50.0% | |
| TATCGAGGAAAATGCCGGCA+AGG | + | 42523:12939-12958 | MS.gene064040:CDS | 50.0% | |
| TGAGGATCCATTTGCCTTGC+CGG | - | 42523:12956-12975 | None:intergenic | 50.0% | |
| CTTTGGATGTGTCGTGTCGG+TGG | + | 42523:11720-11739 | MS.gene064040:intron | 55.0% | |
| GGCATATCCGGTACACGTCA+TGG | - | 42523:11999-12018 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42523 | gene | 10807 | 12999 | 10807 | ID=MS.gene064040 |
| 42523 | mRNA | 10807 | 12999 | 10807 | ID=MS.gene064040.t1;Parent=MS.gene064040 |
| 42523 | exon | 10807 | 11572 | 10807 | ID=MS.gene064040.t1.exon1;Parent=MS.gene064040.t1 |
| 42523 | CDS | 10807 | 11572 | 10807 | ID=cds.MS.gene064040.t1;Parent=MS.gene064040.t1 |
| 42523 | exon | 12309 | 12361 | 12309 | ID=MS.gene064040.t1.exon2;Parent=MS.gene064040.t1 |
| 42523 | CDS | 12309 | 12361 | 12309 | ID=cds.MS.gene064040.t1;Parent=MS.gene064040.t1 |
| 42523 | exon | 12934 | 12999 | 12934 | ID=MS.gene064040.t1.exon3;Parent=MS.gene064040.t1 |
| 42523 | CDS | 12934 | 12999 | 12934 | ID=cds.MS.gene064040.t1;Parent=MS.gene064040.t1 |
| Gene Sequence |
| Protein sequence |
