Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064164.t1 | XP_003609844.1 | 100 | 123 | 0 | 0 | 1 | 123 | 87 | 209 | 7.10E-61 | 243 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064164.t1 | G7JQ12 | 100.0 | 123 | 0 | 0 | 1 | 123 | 87 | 209 | 5.1e-61 | 243.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050786 | MS.gene064164 | 0.813338 | 2.69E-51 | -1.69E-46 |
| MS.gene059028 | MS.gene064164 | 0.810792 | 9.67E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064164.t1 | MTR_4g123630 | 100.000 | 123 | 0 | 0 | 1 | 123 | 87 | 209 | 1.43e-80 | 249 |
| MS.gene064164.t1 | MTR_2g032660 | 59.350 | 123 | 49 | 1 | 2 | 124 | 122 | 243 | 3.95e-35 | 127 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064164.t1 | AT1G48110 | 40.171 | 117 | 57 | 5 | 2 | 108 | 89 | 202 | 9.74e-15 | 69.7 |
| MS.gene064164.t1 | AT1G48110 | 40.171 | 117 | 57 | 5 | 2 | 108 | 89 | 202 | 9.74e-15 | 69.7 |
| MS.gene064164.t1 | AT3G17330 | 46.914 | 81 | 39 | 2 | 2 | 81 | 67 | 144 | 1.55e-12 | 63.2 |
| MS.gene064164.t1 | AT3G17330 | 46.914 | 81 | 39 | 2 | 2 | 81 | 67 | 144 | 1.55e-12 | 63.2 |
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCTGTCAATAGACTTGA+TGG | 0.366272 | 8.4:+11126299 | MS.gene064164:CDS |
| TATATTCCAGGTGCTATGAT+TGG | 0.373959 | 8.4:+11126134 | None:intergenic |
| AATATAAGTTGGCGAAGAAA+TGG | 0.379570 | 8.4:-11126216 | None:intergenic |
| ATTGGAGTTGATGGTTCATT+TGG | 0.388143 | 8.4:+11126152 | MS.gene064164:CDS |
| GCAAAGAAAGATTCGACAAA+TGG | 0.395866 | 8.4:+11126446 | MS.gene064164:CDS |
| ATATAAGTTGGCGAAGAAAT+GGG | 0.400966 | 8.4:-11126215 | None:intergenic |
| TGCTTCATCACCTTCCCATC+AGG | 0.416964 | 8.4:+11126499 | MS.gene064164:CDS |
| ATCAGACAAGTTCCTTCCTC+AGG | 0.430873 | 8.4:+11126393 | MS.gene064164:CDS |
| TTTGCCAAAGCATTAGCTCT+TGG | 0.434436 | 8.4:-11126428 | None:intergenic |
| GGTGCTATGATTGGAGTTGA+TGG | 0.439110 | 8.4:+11126143 | None:intergenic |
| GGAGTTGATGGTTCATTTGG+AGG | 0.459454 | 8.4:+11126155 | MS.gene064164:CDS |
| AGCATTAGCTCTTGGCCCCT+CGG | 0.460855 | 8.4:-11126420 | None:intergenic |
| AAATTAAGAAAACCACTGTT+AGG | 0.473007 | 8.4:-11126476 | None:intergenic |
| GAACTTGTCTGATTTGATGA+TGG | 0.508845 | 8.4:-11126383 | None:intergenic |
| GAACTATGATGAAAATTGTC+TGG | 0.512526 | 8.4:-11126251 | None:intergenic |
| GGGGCCAAGAGCTAATGCTT+TGG | 0.515241 | 8.4:+11126424 | MS.gene064164:CDS |
| GCATTAGCTCTTGGCCCCTC+GGG | 0.520251 | 8.4:-11126419 | None:intergenic |
| TGAACAAGAGGAATATAAGT+TGG | 0.522275 | 8.4:-11126227 | None:intergenic |
| GTCAATAGACTTGATGGAAG+AGG | 0.526422 | 8.4:+11126305 | MS.gene064164:CDS |
| TCCTTCCTCAGGGTACCCGA+GGG | 0.534598 | 8.4:+11126404 | MS.gene064164:CDS |
| TTCCTTCCTCAGGGTACCCG+AGG | 0.539305 | 8.4:+11126403 | MS.gene064164:CDS |
| GGGTAAGCTTATTACCTGAT+GGG | 0.560408 | 8.4:-11126513 | None:intergenic |
| AAATTGTCTGGTTGAACAAG+AGG | 0.565932 | 8.4:-11126239 | None:intergenic |
| TATAAGTTGGCGAAGAAATG+GGG | 0.569498 | 8.4:-11126214 | None:intergenic |
| AAGCTTATTACCTGATGGGA+AGG | 0.591653 | 8.4:-11126509 | None:intergenic |
| CCCCTCGGGTACCCTGAGGA+AGG | 0.595318 | 8.4:-11126405 | None:intergenic |
| TCAGACAAGTTCCTTCCTCA+GGG | 0.638045 | 8.4:+11126394 | MS.gene064164:CDS |
| AATGGAAGTGTTCCTAACAG+TGG | 0.638957 | 8.4:+11126464 | MS.gene064164:CDS |
| TTGGCCCCTCGGGTACCCTG+AGG | 0.673523 | 8.4:-11126409 | None:intergenic |
| CCTTCCTCAGGGTACCCGAG+GGG | 0.684367 | 8.4:+11126405 | MS.gene064164:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTAAGAAAACCACTGTT+AGG | - | chr8.4:11126479-11126498 | None:intergenic | 25.0% |
| GAACTATGATGAAAATTGTC+TGG | - | chr8.4:11126254-11126273 | None:intergenic | 30.0% | |
| TGAACAAGAGGAATATAAGT+TGG | - | chr8.4:11126230-11126249 | None:intergenic | 30.0% | |
| ! | AATATAAGTTGGCGAAGAAA+TGG | - | chr8.4:11126219-11126238 | None:intergenic | 30.0% |
| ! | ATATAAGTTGGCGAAGAAAT+GGG | - | chr8.4:11126218-11126237 | None:intergenic | 30.0% |
| !! | AAATGGGGTTTTGATAATTG+GGG | - | chr8.4:11126202-11126221 | None:intergenic | 30.0% |
| !! | AGAAATGGGGTTTTGATAAT+TGG | - | chr8.4:11126204-11126223 | None:intergenic | 30.0% |
| !! | GAAATGGGGTTTTGATAATT+GGG | - | chr8.4:11126203-11126222 | None:intergenic | 30.0% |
| AAATTGTCTGGTTGAACAAG+AGG | - | chr8.4:11126242-11126261 | None:intergenic | 35.0% | |
| GCAAAGAAAGATTCGACAAA+TGG | + | chr8.4:11126446-11126465 | MS.gene064164:CDS | 35.0% | |
| ! | GAACTTGTCTGATTTGATGA+TGG | - | chr8.4:11126386-11126405 | None:intergenic | 35.0% |
| ! | TATAAGTTGGCGAAGAAATG+GGG | - | chr8.4:11126217-11126236 | None:intergenic | 35.0% |
| !! | ATTGGAGTTGATGGTTCATT+TGG | + | chr8.4:11126152-11126171 | MS.gene064164:CDS | 35.0% |
| GCTTCTGTCAATAGACTTGA+TGG | + | chr8.4:11126299-11126318 | MS.gene064164:CDS | 40.0% | |
| GTCAATAGACTTGATGGAAG+AGG | + | chr8.4:11126305-11126324 | MS.gene064164:CDS | 40.0% | |
| ! | TTTGCCAAAGCATTAGCTCT+TGG | - | chr8.4:11126431-11126450 | None:intergenic | 40.0% |
| !! | AATGGAAGTGTTCCTAACAG+TGG | + | chr8.4:11126464-11126483 | MS.gene064164:CDS | 40.0% |
| ATCAGACAAGTTCCTTCCTC+AGG | + | chr8.4:11126393-11126412 | MS.gene064164:CDS | 45.0% | |
| TCAGACAAGTTCCTTCCTCA+GGG | + | chr8.4:11126394-11126413 | MS.gene064164:CDS | 45.0% | |
| !! | GGAGTTGATGGTTCATTTGG+AGG | + | chr8.4:11126155-11126174 | MS.gene064164:CDS | 45.0% |
| !! | GGGGTTTTGATAATTGGGGA+GGG | - | chr8.4:11126198-11126217 | None:intergenic | 45.0% |
| !! | TGGGGTTTTGATAATTGGGG+AGG | - | chr8.4:11126199-11126218 | None:intergenic | 45.0% |
| !!! | CAGCTTCTGCTGCTTTTACT+AGG | + | chr8.4:11126348-11126367 | MS.gene064164:CDS | 45.0% |
| GGGGCCAAGAGCTAATGCTT+TGG | + | chr8.4:11126424-11126443 | MS.gene064164:CDS | 55.0% | |
| ! | AGCATTAGCTCTTGGCCCCT+CGG | - | chr8.4:11126423-11126442 | None:intergenic | 55.0% |
| ! | GCATTAGCTCTTGGCCCCTC+GGG | - | chr8.4:11126422-11126441 | None:intergenic | 60.0% |
| ! | TCCTTCCTCAGGGTACCCGA+GGG | + | chr8.4:11126404-11126423 | MS.gene064164:CDS | 60.0% |
| ! | TTCCTTCCTCAGGGTACCCG+AGG | + | chr8.4:11126403-11126422 | MS.gene064164:CDS | 60.0% |
| ! | CCTTCCTCAGGGTACCCGAG+GGG | + | chr8.4:11126405-11126424 | MS.gene064164:CDS | 65.0% |
| CCCCTCGGGTACCCTGAGGA+AGG | - | chr8.4:11126408-11126427 | None:intergenic | 70.0% | |
| TTGGCCCCTCGGGTACCCTG+AGG | - | chr8.4:11126412-11126431 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 11126149 | 11126520 | 11126149 | ID=MS.gene064164 |
| chr8.4 | mRNA | 11126149 | 11126520 | 11126149 | ID=MS.gene064164.t1;Parent=MS.gene064164 |
| chr8.4 | exon | 11126149 | 11126520 | 11126149 | ID=MS.gene064164.t1.exon1;Parent=MS.gene064164.t1 |
| chr8.4 | CDS | 11126149 | 11126520 | 11126149 | ID=cds.MS.gene064164.t1;Parent=MS.gene064164.t1 |
| Gene Sequence |
| Protein sequence |
