Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050786.t1 | XP_003626160.2 | 97.9 | 1021 | 20 | 1 | 1 | 1021 | 1 | 1020 | 0.00E+00 | 1761.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050786.t1 | Q9SFU0 | 72.8 | 820 | 219 | 1 | 202 | 1021 | 223 | 1038 | 0.0e+00 | 1251.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050786.t1 | G7KWG6 | 97.9 | 1021 | 20 | 1 | 1 | 1021 | 1 | 1020 | 0.0e+00 | 1761.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050786 | MS.gene050839 | 0.81823 | 2.18E-52 | -1.69E-46 |
| MS.gene050786 | MS.gene053588 | 0.802127 | 6.50E-49 | -1.69E-46 |
| MS.gene050786 | MS.gene055784 | 0.827496 | 1.52E-54 | -1.69E-46 |
| MS.gene050786 | MS.gene055785 | 0.842392 | 2.66E-58 | -1.69E-46 |
| MS.gene050786 | MS.gene056037 | 0.80773 | 4.38E-50 | -1.69E-46 |
| MS.gene050786 | MS.gene057127 | 0.833733 | 4.50E-56 | -1.69E-46 |
| MS.gene050786 | MS.gene060810 | 0.841671 | 4.12E-58 | -1.69E-46 |
| MS.gene050786 | MS.gene061710 | 0.826504 | 2.62E-54 | -1.69E-46 |
| MS.gene050786 | MS.gene061859 | 0.82926 | 5.68E-55 | -1.69E-46 |
| MS.gene050786 | MS.gene063482 | 0.820025 | 8.53E-53 | -1.69E-46 |
| MS.gene050786 | MS.gene064164 | 0.813338 | 2.69E-51 | -1.69E-46 |
| MS.gene050786 | MS.gene064489 | 0.815268 | 1.01E-51 | -1.69E-46 |
| MS.gene050786 | MS.gene065040 | 0.838637 | 2.55E-57 | -1.69E-46 |
| MS.gene050786 | MS.gene06684 | 0.828145 | 1.06E-54 | -1.69E-46 |
| MS.gene050786 | MS.gene06895 | 0.839837 | 1.25E-57 | -1.69E-46 |
| MS.gene050786 | MS.gene072583 | 0.960729 | 6.90E-119 | -1.69E-46 |
| MS.gene050786 | MS.gene072642 | 0.833305 | 5.76E-56 | -1.69E-46 |
| MS.gene050786 | MS.gene073113 | 0.810526 | 1.10E-50 | -1.69E-46 |
| MS.gene050786 | MS.gene24018 | 0.819879 | 9.21E-53 | -1.69E-46 |
| MS.gene050786 | MS.gene24293 | 0.80779 | 4.25E-50 | -1.69E-46 |
| MS.gene050786 | MS.gene33771 | 0.829206 | 5.86E-55 | -1.69E-46 |
| MS.gene050786 | MS.gene36854 | 0.833793 | 4.35E-56 | -1.69E-46 |
| MS.gene050786 | MS.gene44059 | 0.816782 | 4.63E-52 | -1.69E-46 |
| MS.gene050786 | MS.gene44096 | 0.825112 | 5.60E-54 | -1.69E-46 |
| MS.gene050786 | MS.gene58406 | 0.833022 | 6.77E-56 | -1.69E-46 |
| MS.gene050786 | MS.gene59960 | 0.833438 | 5.33E-56 | -1.69E-46 |
| MS.gene050786 | MS.gene60145 | 0.804018 | 2.64E-49 | -1.69E-46 |
| MS.gene050786 | MS.gene64074 | 0.85656 | 2.96E-62 | -1.69E-46 |
| MS.gene050786 | MS.gene65381 | 0.821521 | 3.86E-53 | -1.69E-46 |
| MS.gene050786 | MS.gene69171 | 0.801255 | 9.81E-49 | -1.69E-46 |
| MS.gene050786 | MS.gene74958 | 0.875618 | 2.67E-68 | -1.69E-46 |
| MS.gene050786 | MS.gene76208 | 0.853555 | 2.21E-61 | -1.69E-46 |
| MS.gene050786 | MS.gene77452 | 0.856105 | 4.03E-62 | -1.69E-46 |
| MS.gene050786 | MS.gene88038 | 0.810445 | 1.15E-50 | -1.69E-46 |
| MS.gene050786 | MS.gene94813 | 0.826932 | 2.07E-54 | -1.69E-46 |
| MS.gene050786 | MS.gene99193 | 0.826611 | 2.47E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene050786 | MS.gene75647 | PPI |
| MS.gene050786 | MS.gene79918 | PPI |
| MS.gene050786 | MS.gene045327 | PPI |
| MS.gene050786 | MS.gene77559 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050786.t1 | MTR_7g111710 | 98.531 | 1021 | 14 | 1 | 1 | 1021 | 1 | 1020 | 0.0 | 2060 |
| MS.gene050786.t1 | MTR_1g112310 | 34.859 | 1027 | 577 | 22 | 10 | 1020 | 157 | 1107 | 0.0 | 556 |
| MS.gene050786.t1 | MTR_6g042060 | 88.406 | 69 | 8 | 0 | 603 | 671 | 1 | 69 | 1.22e-35 | 130 |
| MS.gene050786.t1 | MTR_1g068945 | 89.286 | 56 | 6 | 0 | 616 | 671 | 1 | 56 | 6.30e-29 | 110 |
| MS.gene050786.t1 | MTR_8g096550 | 90.244 | 41 | 4 | 0 | 631 | 671 | 11 | 51 | 5.44e-19 | 82.0 |
| MS.gene050786.t1 | MTR_4g052930 | 22.280 | 579 | 357 | 22 | 304 | 816 | 3 | 554 | 2.86e-17 | 87.4 |
| MS.gene050786.t1 | MTR_7g110640 | 21.598 | 676 | 435 | 26 | 298 | 933 | 134 | 754 | 6.33e-11 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050786.t1 | AT3G07100 | 63.860 | 1057 | 327 | 11 | 1 | 1021 | 1 | 1038 | 0.0 | 1316 |
| MS.gene050786.t1 | AT3G44340 | 36.874 | 819 | 476 | 13 | 227 | 1020 | 291 | 1093 | 5.81e-171 | 530 |
| MS.gene050786.t1 | AT3G44340 | 36.874 | 819 | 476 | 13 | 227 | 1020 | 291 | 1093 | 5.81e-171 | 530 |
| MS.gene050786.t1 | AT3G44340 | 36.874 | 819 | 476 | 13 | 227 | 1020 | 291 | 1093 | 5.81e-171 | 530 |
| MS.gene050786.t1 | AT4G32640 | 34.778 | 1038 | 584 | 23 | 45 | 1019 | 69 | 1076 | 1.05e-167 | 521 |
| MS.gene050786.t1 | AT4G32640 | 34.778 | 1038 | 584 | 23 | 45 | 1019 | 69 | 1076 | 1.05e-167 | 521 |
| MS.gene050786.t1 | AT3G44340 | 36.650 | 794 | 463 | 12 | 227 | 995 | 291 | 1069 | 2.15e-162 | 506 |
| MS.gene050786.t1 | AT3G44340 | 36.650 | 794 | 463 | 12 | 227 | 995 | 291 | 1069 | 2.15e-162 | 506 |
| MS.gene050786.t1 | AT2G27460 | 21.875 | 576 | 360 | 21 | 304 | 815 | 3 | 552 | 1.00e-16 | 85.9 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51799 | gene | 17214 | 27374 | 17214 | ID=MS.gene050786 |
| 51799 | mRNA | 17214 | 27374 | 17214 | ID=MS.gene050786.t1;Parent=MS.gene050786 |
| 51799 | exon | 17214 | 18227 | 17214 | ID=MS.gene050786.t1.exon1;Parent=MS.gene050786.t1 |
| 51799 | CDS | 17214 | 18227 | 17214 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 18311 | 18449 | 18311 | ID=MS.gene050786.t1.exon2;Parent=MS.gene050786.t1 |
| 51799 | CDS | 18311 | 18449 | 18311 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 18781 | 18971 | 18781 | ID=MS.gene050786.t1.exon3;Parent=MS.gene050786.t1 |
| 51799 | CDS | 18781 | 18971 | 18781 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 19753 | 19860 | 19753 | ID=MS.gene050786.t1.exon4;Parent=MS.gene050786.t1 |
| 51799 | CDS | 19753 | 19860 | 19753 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 19981 | 20184 | 19981 | ID=MS.gene050786.t1.exon5;Parent=MS.gene050786.t1 |
| 51799 | CDS | 19981 | 20184 | 19981 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 23833 | 24064 | 23833 | ID=MS.gene050786.t1.exon6;Parent=MS.gene050786.t1 |
| 51799 | CDS | 23833 | 24064 | 23833 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 24494 | 24643 | 24494 | ID=MS.gene050786.t1.exon7;Parent=MS.gene050786.t1 |
| 51799 | CDS | 24494 | 24643 | 24494 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 24787 | 24943 | 24787 | ID=MS.gene050786.t1.exon8;Parent=MS.gene050786.t1 |
| 51799 | CDS | 24787 | 24943 | 24787 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 25676 | 25803 | 25676 | ID=MS.gene050786.t1.exon9;Parent=MS.gene050786.t1 |
| 51799 | CDS | 25676 | 25803 | 25676 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 25879 | 26201 | 25879 | ID=MS.gene050786.t1.exon10;Parent=MS.gene050786.t1 |
| 51799 | CDS | 25879 | 26201 | 25879 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 26279 | 26467 | 26279 | ID=MS.gene050786.t1.exon11;Parent=MS.gene050786.t1 |
| 51799 | CDS | 26279 | 26467 | 26279 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| 51799 | exon | 27144 | 27374 | 27144 | ID=MS.gene050786.t1.exon12;Parent=MS.gene050786.t1 |
| 51799 | CDS | 27144 | 27374 | 27144 | ID=cds.MS.gene050786.t1;Parent=MS.gene050786.t1 |
| Gene Sequence |
| Protein sequence |
