Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064399.t1 | XP_013459842.1 | 98.7 | 305 | 2 | 1 | 3 | 305 | 2 | 306 | 1.60E-162 | 582 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064399.t1 | A7MB47 | 71.5 | 270 | 77 | 0 | 26 | 295 | 24 | 293 | 2.4e-106 | 386.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064399.t1 | A0A072UXT2 | 98.7 | 305 | 2 | 1 | 3 | 305 | 2 | 306 | 1.1e-162 | 582.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene064399.t1 | TR | Rcd1-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050750 | MS.gene064399 | -0.805584 | 1.24E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene064399 | -0.806774 | 6.99E-50 | -1.69E-46 |
| MS.gene054405 | MS.gene064399 | -0.802924 | 4.45E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene064399 | -0.822084 | 2.86E-53 | -1.69E-46 |
| MS.gene060137 | MS.gene064399 | 0.816196 | 6.26E-52 | -1.69E-46 |
| MS.gene060802 | MS.gene064399 | -0.806942 | 6.44E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064399.t1 | MTR_3g053170 | 98.689 | 305 | 2 | 1 | 3 | 305 | 2 | 306 | 0.0 | 610 |
| MS.gene064399.t1 | MTR_8g106940 | 60.131 | 306 | 119 | 1 | 1 | 303 | 11 | 316 | 1.52e-122 | 354 |
| MS.gene064399.t1 | MTR_6g065170 | 61.000 | 300 | 114 | 1 | 1 | 297 | 11 | 310 | 1.46e-120 | 349 |
| MS.gene064399.t1 | MTR_5g093460 | 59.643 | 280 | 112 | 1 | 17 | 296 | 159 | 437 | 1.06e-110 | 328 |
| MS.gene064399.t1 | MTR_5g093460 | 65.672 | 134 | 45 | 1 | 21 | 154 | 1 | 133 | 3.64e-50 | 172 |
| MS.gene064399.t1 | MTR_3g018640 | 57.093 | 289 | 120 | 1 | 4 | 288 | 13 | 301 | 5.71e-108 | 316 |
| MS.gene064399.t1 | MTR_1g056470 | 67.257 | 226 | 74 | 0 | 19 | 244 | 28 | 253 | 9.61e-106 | 309 |
| MS.gene064399.t1 | MTR_1g043540 | 56.000 | 125 | 54 | 1 | 129 | 253 | 9 | 132 | 8.48e-37 | 128 |
| MS.gene064399.t1 | MTR_1g043500 | 49.206 | 63 | 31 | 1 | 183 | 244 | 3 | 65 | 4.06e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064399.t1 | AT3G20800 | 85.333 | 300 | 42 | 2 | 5 | 303 | 18 | 316 | 0.0 | 519 |
| MS.gene064399.t1 | AT5G12980 | 79.443 | 287 | 53 | 2 | 20 | 305 | 30 | 311 | 1.99e-168 | 470 |
| MS.gene064399.t1 | AT2G32550 | 34.432 | 273 | 163 | 5 | 26 | 285 | 51 | 320 | 9.55e-44 | 152 |
| MS.gene064399.t1 | AT2G32550 | 35.060 | 251 | 149 | 4 | 46 | 285 | 34 | 281 | 1.94e-42 | 147 |
| MS.gene064399.t1 | AT2G32550 | 34.934 | 229 | 135 | 4 | 68 | 285 | 1 | 226 | 2.58e-37 | 133 |
Find 66 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGCTGTTGGTCGAGTTCT+TGG | 0.255938 | 3.3:+44055758 | MS.gene064399:CDS |
| AGGAATAGCATCCTTGTATC+AGG | 0.295510 | 3.3:-44053427 | None:intergenic |
| TGGATTTGCTTGTTGTGTTC+AGG | 0.306518 | 3.3:-44054401 | None:intergenic |
| TGCAGCCACTTCCTAGTTGT+TGG | 0.322430 | 3.3:-44056647 | None:intergenic |
| TCCGCTGTGCCTGCGCACTA+TGG | 0.351143 | 3.3:+44054900 | MS.gene064399:CDS |
| AGAAAGTAACATTACCATTC+AGG | 0.352299 | 3.3:-44053447 | None:intergenic |
| GCAGCCACTTCCTAGTTGTT+GGG | 0.391810 | 3.3:-44056646 | None:intergenic |
| CCATTGCTATGGAATTCATT+TGG | 0.401738 | 3.3:+44051895 | MS.gene064399:CDS |
| AACAACAACTCTTCTTCATT+CGG | 0.406492 | 3.3:+44049206 | MS.gene064399:CDS |
| GATTGTGCTGGAGTGAGGTT+GGG | 0.411334 | 3.3:-44052849 | None:intergenic |
| TCTGTTAGACGATGTGGGCT+TGG | 0.418307 | 3.3:+44055706 | MS.gene064399:CDS |
| TGATTGTGCTGGAGTGAGGT+TGG | 0.420676 | 3.3:-44052850 | None:intergenic |
| GTGCCTGCGCACTATGGAGA+TGG | 0.432141 | 3.3:+44054906 | MS.gene064399:CDS |
| GCTGTCTTCCTGACATGTTA+AGG | 0.435901 | 3.3:+44056028 | MS.gene064399:CDS |
| CCTCCTCCACCTTGCAGTGC+AGG | 0.438234 | 3.3:-44056704 | None:intergenic |
| CACACTCGATTTGATTGTGC+TGG | 0.438779 | 3.3:-44052861 | None:intergenic |
| TACTTGAGGCTTACTAGTCT+TGG | 0.443121 | 3.3:+44054435 | MS.gene064399:CDS |
| CAAATGAATTCCATAGCAAT+GGG | 0.454592 | 3.3:-44051894 | None:intergenic |
| TTGGAAGAAGCGGGAGAAGA+AGG | 0.455297 | 3.3:-44049236 | None:intergenic |
| CAATGCTCTTGCCCTGCTCC+AGG | 0.456652 | 3.3:+44052884 | MS.gene064399:CDS |
| TACTATTGCAGCACTTCTGC+AGG | 0.460816 | 3.3:+44051918 | MS.gene064399:CDS |
| CCTCAAGTACTCAAAAGGTC+TGG | 0.463934 | 3.3:-44054421 | None:intergenic |
| CTGTCTTCCTGACATGTTAA+GGG | 0.464598 | 3.3:+44056029 | MS.gene064399:CDS |
| TCCCGCTTCTTCCAACTCGA+TGG | 0.477236 | 3.3:+44049244 | MS.gene064399:CDS |
| TGCCTGCGCACTATGGAGAT+GGG | 0.477491 | 3.3:+44054907 | MS.gene064399:CDS |
| AAGATCTCGCCCCATTGCTA+TGG | 0.486631 | 3.3:+44051884 | MS.gene064399:CDS |
| GTAAGCCTCAAGTACTCAAA+AGG | 0.495117 | 3.3:-44054426 | None:intergenic |
| GTAGCATCCCTTAACATGTC+AGG | 0.500922 | 3.3:-44056036 | None:intergenic |
| CGCTCTTCTCGAACTCTCCA+AGG | 0.503850 | 3.3:+44049319 | MS.gene064399:CDS |
| CCTGCACTGCAAGGTGGAGG+AGG | 0.510437 | 3.3:+44056704 | MS.gene064399:CDS |
| AAACTCATCTAACCTGGAGC+AGG | 0.510559 | 3.3:-44052896 | None:intergenic |
| CAACAACTGATTCACAATGT+TGG | 0.517621 | 3.3:+44056668 | MS.gene064399:CDS |
| CCAAATGAATTCCATAGCAA+TGG | 0.523846 | 3.3:-44051895 | None:intergenic |
| AGCCATCGAGTTGGAAGAAG+CGG | 0.531152 | 3.3:-44049246 | None:intergenic |
| AAAGATTCTGTTAGACGATG+TGG | 0.532417 | 3.3:+44055700 | MS.gene064399:CDS |
| TTGCTTGTTGTGTTCAGGAA+CGG | 0.537424 | 3.3:-44054396 | None:intergenic |
| CTTACTAGTCTTGGTGTCAT+CGG | 0.543029 | 3.3:+44054444 | MS.gene064399:CDS |
| GGGGAGTGAGCTCTCTAAAA+CGG | 0.547334 | 3.3:+44054927 | MS.gene064399:CDS |
| GTTCCTGCACTGCAAGGTGG+AGG | 0.551104 | 3.3:+44056701 | MS.gene064399:CDS |
| TAGCATCTCACCCTGATACA+AGG | 0.553761 | 3.3:+44053416 | MS.gene064399:CDS |
| ACAAGGATGCTATTCCTGAA+TGG | 0.556336 | 3.3:+44053433 | MS.gene064399:CDS |
| GCCATCGAGTTGGAAGAAGC+GGG | 0.561952 | 3.3:-44049245 | None:intergenic |
| TGCTTCAACAGACGAGATGA+TGG | 0.564466 | 3.3:-44055809 | None:intergenic |
| TCCCCATCTCCATAGTGCGC+AGG | 0.574764 | 3.3:-44054909 | None:intergenic |
| AACTGATTCACAATGTTGGA+GGG | 0.575243 | 3.3:+44056672 | MS.gene064399:CDS |
| TTCAGAAGAAGCCATCGAGT+TGG | 0.578031 | 3.3:-44049255 | None:intergenic |
| AGGTTGGGAGGAGACAAAAC+AGG | 0.578541 | 3.3:-44052834 | None:intergenic |
| CAACTGATTCACAATGTTGG+AGG | 0.581651 | 3.3:+44056671 | MS.gene064399:CDS |
| TTCAGGAGGACCCAACAACT+AGG | 0.584464 | 3.3:+44056636 | MS.gene064399:intron |
| AAGATTCTGTTAGACGATGT+GGG | 0.593200 | 3.3:+44055701 | MS.gene064399:CDS |
| AGGACCCAACAACTAGGAAG+TGG | 0.596471 | 3.3:+44056642 | MS.gene064399:CDS |
| TGTGCTGGAGTGAGGTTGGG+AGG | 0.609651 | 3.3:-44052846 | None:intergenic |
| AATCGAGTTCCTGCACTGCA+AGG | 0.621933 | 3.3:+44056695 | MS.gene064399:CDS |
| TGGTCGAGTTCTTGGAAACA+TGG | 0.623015 | 3.3:+44055766 | MS.gene064399:CDS |
| GATTTGATTGTGCTGGAGTG+AGG | 0.624347 | 3.3:-44052854 | None:intergenic |
| AACTCATCTAACCTGGAGCA+GGG | 0.624665 | 3.3:-44052895 | None:intergenic |
| GGAATAGCATCCTTGTATCA+GGG | 0.625986 | 3.3:-44053426 | None:intergenic |
| TTCGTCTGTCAGATAACCCG+AGG | 0.629082 | 3.3:+44055849 | MS.gene064399:CDS |
| TGTCATCGGTGCACTAGTGA+AGG | 0.630356 | 3.3:+44054458 | MS.gene064399:CDS |
| GCCTGCGCACTATGGAGATG+GGG | 0.643265 | 3.3:+44054908 | MS.gene064399:CDS |
| CGAGTTCCTGCACTGCAAGG+TGG | 0.656169 | 3.3:+44056698 | MS.gene064399:CDS |
| TCCATAGTGCGCAGGCACAG+CGG | 0.666861 | 3.3:-44054901 | None:intergenic |
| TCACCAGGTTGATGATACTG+AGG | 0.679946 | 3.3:+44054846 | MS.gene064399:intron |
| TCGAGTTCTTGGAAACATGG+TGG | 0.707168 | 3.3:+44055769 | MS.gene064399:CDS |
| AGGAACGGATACAAATACAG+AGG | 0.734864 | 3.3:-44054381 | None:intergenic |
| AAATGAATTCCATAGCAATG+GGG | 0.758849 | 3.3:-44051893 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 44049203 | 44056751 | 44049203 | ID=MS.gene064399 |
| chr3.3 | mRNA | 44049203 | 44056751 | 44049203 | ID=MS.gene064399.t1;Parent=MS.gene064399 |
| chr3.3 | exon | 44049203 | 44049340 | 44049203 | ID=MS.gene064399.t1.exon1;Parent=MS.gene064399.t1 |
| chr3.3 | CDS | 44049203 | 44049340 | 44049203 | ID=cds.MS.gene064399.t1;Parent=MS.gene064399.t1 |
| chr3.3 | exon | 44051868 | 44051939 | 44051868 | ID=MS.gene064399.t1.exon2;Parent=MS.gene064399.t1 |
| chr3.3 | CDS | 44051868 | 44051939 | 44051868 | ID=cds.MS.gene064399.t1;Parent=MS.gene064399.t1 |
| chr3.3 | exon | 44052816 | 44052905 | 44052816 | ID=MS.gene064399.t1.exon3;Parent=MS.gene064399.t1 |
| chr3.3 | CDS | 44052816 | 44052905 | 44052816 | ID=cds.MS.gene064399.t1;Parent=MS.gene064399.t1 |
| chr3.3 | exon | 44053412 | 44053454 | 44053412 | ID=MS.gene064399.t1.exon4;Parent=MS.gene064399.t1 |
| chr3.3 | CDS | 44053412 | 44053454 | 44053412 | ID=cds.MS.gene064399.t1;Parent=MS.gene064399.t1 |
| chr3.3 | exon | 44054373 | 44054479 | 44054373 | ID=MS.gene064399.t1.exon5;Parent=MS.gene064399.t1 |
| chr3.3 | CDS | 44054373 | 44054479 | 44054373 | ID=cds.MS.gene064399.t1;Parent=MS.gene064399.t1 |
| chr3.3 | exon | 44054853 | 44054948 | 44054853 | ID=MS.gene064399.t1.exon6;Parent=MS.gene064399.t1 |
| chr3.3 | CDS | 44054853 | 44054948 | 44054853 | ID=cds.MS.gene064399.t1;Parent=MS.gene064399.t1 |
| chr3.3 | exon | 44055680 | 44055870 | 44055680 | ID=MS.gene064399.t1.exon7;Parent=MS.gene064399.t1 |
| chr3.3 | CDS | 44055680 | 44055870 | 44055680 | ID=cds.MS.gene064399.t1;Parent=MS.gene064399.t1 |
| chr3.3 | exon | 44056008 | 44056077 | 44056008 | ID=MS.gene064399.t1.exon8;Parent=MS.gene064399.t1 |
| chr3.3 | CDS | 44056008 | 44056077 | 44056008 | ID=cds.MS.gene064399.t1;Parent=MS.gene064399.t1 |
| chr3.3 | exon | 44056641 | 44056751 | 44056641 | ID=MS.gene064399.t1.exon9;Parent=MS.gene064399.t1 |
| chr3.3 | CDS | 44056641 | 44056751 | 44056641 | ID=cds.MS.gene064399.t1;Parent=MS.gene064399.t1 |
| Gene Sequence |
| Protein sequence |
