AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064526

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064526.t1 MTR_6g090290 91.284 436 38 0 1 436 1 436 0.0 789
MS.gene064526.t1 MTR_8g096990 70.252 437 125 2 1 437 1 432 0.0 630
MS.gene064526.t1 MTR_5g080890 69.476 439 127 5 1 437 1 434 0.0 616
MS.gene064526.t1 MTR_5g080960 69.704 439 126 5 1 437 1 434 0.0 616
MS.gene064526.t1 MTR_8g038280 66.819 437 144 1 1 437 1 436 0.0 589
MS.gene064526.t1 MTR_5g044770 64.989 437 147 3 1 437 1 431 0.0 567
MS.gene064526.t1 MTR_8g038250 63.205 443 130 4 1 437 1 416 0.0 557
MS.gene064526.t1 MTR_8g038310 58.124 437 158 5 1 436 1 413 4.80e-174 493
MS.gene064526.t1 MTR_4g109760 61.556 437 130 6 1 437 1 399 1.27e-171 487
MS.gene064526.t1 MTR_3g028120 49.763 422 193 9 27 437 26 439 2.12e-137 401
MS.gene064526.t1 MTR_5g038360 47.597 437 212 10 9 436 8 436 2.93e-130 383
MS.gene064526.t1 MTR_6g088760 49.878 411 189 10 6 407 7 409 1.27e-128 382
MS.gene064526.t1 MTR_2g067830 48.786 412 198 9 32 436 1 406 7.73e-121 358
MS.gene064526.t1 MTR_0024s0040 48.325 418 197 10 30 436 1 410 3.38e-120 356
MS.gene064526.t1 MTR_5g044570 43.498 446 207 10 1 436 1 411 2.27e-115 344
MS.gene064526.t1 MTR_2g105300 46.455 409 184 11 32 433 1 381 5.41e-109 327
MS.gene064526.t1 MTR_0036s0050 40.899 445 232 13 9 436 5 435 4.29e-104 316
MS.gene064526.t1 MTR_0036s0070 39.374 447 239 12 1 436 1 426 1.12e-103 315
MS.gene064526.t1 MTR_2g059490 42.318 371 200 10 75 437 47 411 1.25e-91 284
MS.gene064526.t1 MTR_5g008070 40.521 422 225 10 21 435 23 425 2.93e-91 283
MS.gene064526.t1 MTR_0036s0110 37.118 458 250 12 1 436 1 442 6.47e-91 282
MS.gene064526.t1 MTR_5g008150 39.637 386 216 9 59 436 36 412 4.22e-90 279
MS.gene064526.t1 MTR_2g059460 39.762 420 203 14 26 436 28 406 3.11e-89 277
MS.gene064526.t1 MTR_5g008110 39.037 374 212 8 70 436 51 415 1.14e-87 273
MS.gene064526.t1 MTR_3g097150 33.481 451 264 11 6 437 16 449 6.20e-75 241
MS.gene064526.t1 MTR_1g060750 34.152 407 226 11 40 436 2 376 1.92e-64 212
MS.gene064526.t1 MTR_4g045857 44.130 247 129 7 74 315 46 288 1.50e-57 191
MS.gene064526.t1 MTR_5g008090 35.855 304 178 8 141 436 20 314 2.19e-55 186
MS.gene064526.t1 MTR_1g115210 33.100 429 257 11 21 434 73 486 2.87e-55 191
MS.gene064526.t1 MTR_7g117430 32.798 436 254 13 19 434 68 484 5.15e-55 190
MS.gene064526.t1 MTR_1g039270 33.180 434 241 17 28 434 58 469 8.74e-55 189
MS.gene064526.t1 MTR_3g046300 33.505 388 235 11 68 437 79 461 2.79e-46 166
MS.gene064526.t1 MTR_1g111350 33.884 363 202 14 85 434 130 467 1.52e-45 164
MS.gene064526.t1 MTR_2g028190 30.736 462 278 17 4 434 1 451 3.30e-44 160
MS.gene064526.t1 MTR_6g085010 32.861 353 216 10 86 434 156 491 1.48e-43 159
MS.gene064526.t1 MTR_3g053710 31.593 364 222 10 85 434 116 466 4.02e-42 155
MS.gene064526.t1 MTR_8g103880 30.851 376 237 12 66 434 96 455 8.59e-42 154
MS.gene064526.t1 MTR_6g088765 39.331 239 106 6 162 399 5 205 2.11e-41 147
MS.gene064526.t1 MTR_4g007740 30.175 401 250 11 49 435 94 478 8.81e-41 151
MS.gene064526.t1 MTR_5g091130 30.591 389 246 10 68 437 173 556 1.27e-40 152
MS.gene064526.t1 MTR_2g055840 27.273 374 232 12 87 434 14 373 9.27e-33 127
MS.gene064526.t1 MTR_3g053980 30.769 364 219 9 85 434 141 485 4.19e-31 125
MS.gene064526.t1 MTR_4g008110 54.167 120 28 1 158 277 45 137 6.93e-29 110
MS.gene064526.t1 MTR_2g070430 26.216 473 293 20 2 436 3 457 8.76e-28 115
MS.gene064526.t1 MTR_1g078240 26.110 383 231 15 85 435 68 430 3.68e-27 113
MS.gene064526.t1 MTR_7g062360 27.136 398 244 16 72 437 45 428 2.73e-26 110
MS.gene064526.t1 MTR_7g080230 24.215 446 273 16 30 435 11 431 3.05e-26 110
MS.gene064526.t1 MTR_3g068000 26.425 386 224 13 89 434 76 441 4.07e-26 110
MS.gene064526.t1 MTR_4g095270 25.000 416 267 17 41 437 37 426 7.32e-26 110
MS.gene064526.t1 MTR_4g095270 25.000 416 267 17 41 437 37 426 7.99e-26 110
MS.gene064526.t1 MTR_1g078240 26.042 384 231 16 85 435 68 431 1.36e-25 108
MS.gene064526.t1 MTR_8g075090 27.513 378 217 16 87 436 113 461 1.43e-23 103
MS.gene064526.t1 MTR_5g016260 23.656 372 241 15 85 437 87 434 1.54e-23 103
MS.gene064526.t1 MTR_5g016260 23.656 372 241 15 85 437 87 434 1.64e-23 103
MS.gene064526.t1 MTR_2g063110 25.250 400 241 13 85 434 117 508 1.93e-23 102
MS.gene064526.t1 MTR_3g065160 24.416 385 235 14 83 437 71 429 2.52e-23 102
MS.gene064526.t1 MTR_2g084550 27.749 382 226 16 85 435 76 438 3.62e-23 102
MS.gene064526.t1 MTR_4g056420 26.904 394 219 19 82 435 73 437 3.95e-23 101
MS.gene064526.t1 MTR_8g094590 25.459 381 231 13 85 437 72 427 5.60e-23 100
MS.gene064526.t1 MTR_8g094590 29.720 286 164 10 85 358 72 332 1.84e-22 99.4
MS.gene064526.t1 MTR_8g094590 31.061 264 151 9 85 342 72 310 1.91e-22 97.4
MS.gene064526.t1 MTR_8g075090 27.177 379 213 17 87 434 113 459 2.42e-22 99.4
MS.gene064526.t1 MTR_1g037630 25.587 426 278 16 26 435 27 429 2.65e-22 99.0
MS.gene064526.t1 MTR_8g039540 27.716 451 283 18 6 435 6 434 2.73e-22 99.0
MS.gene064526.t1 MTR_1g061940 24.416 471 281 22 8 435 7 445 3.19e-22 99.4
MS.gene064526.t1 MTR_7g021510 23.560 382 243 13 85 435 71 434 3.68e-22 99.0
MS.gene064526.t1 MTR_1g029810 26.042 384 226 19 85 437 56 412 4.76e-22 98.2
MS.gene064526.t1 MTR_5g061690 29.111 371 211 19 78 422 89 433 6.49e-22 98.6
MS.gene064526.t1 MTR_1g029810 25.521 384 228 18 85 437 56 412 6.89e-22 97.8
MS.gene064526.t1 MTR_7g105850 25.255 491 274 22 9 436 7 467 1.36e-21 97.1
MS.gene064526.t1 MTR_0945s0010 37.795 127 74 3 314 437 1 125 2.57e-21 90.5
MS.gene064526.t1 MTR_3g087780 25.383 457 272 20 24 437 23 453 3.02e-21 96.7
MS.gene064526.t1 MTR_3g014290 28.134 359 226 13 87 435 81 417 3.12e-21 95.5
MS.gene064526.t1 MTR_1g078240 27.129 317 187 13 85 377 68 364 5.93e-21 94.4
MS.gene064526.t1 MTR_8g075090 27.714 350 198 15 87 409 113 434 7.07e-21 95.1
MS.gene064526.t1 MTR_7g102750 25.191 393 221 19 85 435 80 441 7.52e-21 95.1
MS.gene064526.t1 MTR_2g103340 40.000 125 30 2 21 145 34 113 3.53e-20 88.2
MS.gene064526.t1 MTR_6g084710 24.490 392 251 14 71 434 176 550 8.14e-20 92.4
MS.gene064526.t1 MTR_3g096930 24.219 384 231 16 85 437 83 437 1.59e-19 90.9
MS.gene064526.t1 MTR_6g084710 26.246 301 194 11 71 360 176 459 1.12e-18 88.6
MS.gene064526.t1 MTR_5g022880 25.266 376 226 14 87 434 113 461 4.32e-18 87.0
MS.gene064526.t1 MTR_5g077850 22.798 386 244 14 83 437 78 440 1.87e-17 84.7
MS.gene064526.t1 MTR_7g080230 23.653 334 207 13 129 435 49 361 6.74e-17 82.8
MS.gene064526.t1 MTR_4g023630 24.485 388 235 14 84 437 59 422 1.28e-16 82.0
MS.gene064526.t1 MTR_4g100990 25.236 424 237 18 78 437 66 473 1.44e-16 82.0
MS.gene064526.t1 MTR_7g102750 25.532 329 178 16 85 377 80 377 3.41e-16 80.9
MS.gene064526.t1 MTR_5g061690 28.956 297 174 15 138 422 23 294 3.50e-16 80.1
MS.gene064526.t1 MTR_4g023650 24.679 389 233 18 84 437 59 422 9.95e-16 79.3
MS.gene064526.t1 MTR_3g087780 25.846 325 205 12 128 437 44 347 1.18e-15 79.0
MS.gene064526.t1 MTR_3g087780 25.846 325 205 12 128 437 8 311 1.24e-15 78.6
MS.gene064526.t1 MTR_5g077920 23.907 389 250 14 76 436 58 428 1.72e-15 78.6
MS.gene064526.t1 MTR_5g012490 25.806 496 271 26 6 435 6 470 1.89e-15 78.6
MS.gene064526.t1 MTR_8g075010 23.913 368 235 14 93 437 107 452 3.18e-15 78.2
MS.gene064526.t1 MTR_2g037710 27.570 214 126 8 227 434 15 205 2.68e-12 66.2
MS.gene064526.t1 MTR_1g093100 24.936 393 232 19 67 419 17 386 2.36e-11 65.9
MS.gene064526.t1 MTR_3g100500 25.952 420 259 21 40 434 45 437 2.99e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064526.t1 AT1G64830 46.988 415 212 4 25 437 23 431 1.23e-131 386
MS.gene064526.t1 AT5G33340 47.101 414 210 5 27 437 30 437 3.35e-124 367
MS.gene064526.t1 AT2G35615 42.921 445 232 8 7 435 6 444 1.99e-110 333
MS.gene064526.t1 AT1G31450 41.685 451 238 10 1 435 1 442 2.07e-104 317
MS.gene064526.t1 AT2G28010 37.130 439 220 11 5 437 6 394 1.35e-80 254
MS.gene064526.t1 AT2G28040 38.084 428 207 15 16 437 18 393 1.33e-76 244
MS.gene064526.t1 AT2G28040 38.609 417 198 15 27 437 20 384 2.20e-76 243
MS.gene064526.t1 AT2G28030 40.056 357 181 9 87 437 61 390 1.42e-74 239
MS.gene064526.t1 AT2G28220 35.963 431 221 10 15 437 17 400 3.55e-73 235
MS.gene064526.t1 AT2G03200 35.023 434 247 10 25 437 42 461 3.22e-72 234
MS.gene064526.t1 AT2G28225 35.714 378 209 8 69 437 48 400 3.50e-67 220
MS.gene064526.t1 AT1G01300 36.813 364 199 12 85 435 140 485 1.04e-54 189
MS.gene064526.t1 AT3G61820 34.783 391 229 11 55 434 108 483 3.63e-52 182
MS.gene064526.t1 AT4G30030 33.777 376 221 16 70 434 61 419 8.85e-51 177
MS.gene064526.t1 AT2G23945 28.200 461 280 16 9 437 10 451 1.33e-50 177
MS.gene064526.t1 AT3G20015 30.023 443 259 15 20 434 51 470 3.61e-48 171
MS.gene064526.t1 AT3G18490 31.591 421 242 13 23 434 117 500 5.99e-47 169
MS.gene064526.t1 AT5G10770 33.241 361 215 9 85 435 130 474 4.03e-46 166
MS.gene064526.t1 AT1G25510 33.889 360 206 12 85 434 146 483 5.16e-45 163
MS.gene064526.t1 AT5G10760 33.756 394 227 11 57 434 89 464 2.02e-44 161
MS.gene064526.t1 AT4G30040 29.730 444 263 17 9 429 10 427 5.01e-42 154
MS.gene064526.t1 AT1G79720 33.062 369 207 15 86 434 134 482 3.17e-40 150
MS.gene064526.t1 AT3G25700 28.176 433 254 15 39 435 39 450 6.86e-40 149
MS.gene064526.t1 AT2G42980 30.206 437 264 17 31 437 102 527 8.56e-40 150
MS.gene064526.t1 AT5G36260 29.787 376 222 14 85 435 76 434 7.95e-38 144
MS.gene064526.t1 AT3G59080 28.827 392 242 13 72 437 155 535 5.07e-36 139
MS.gene064526.t1 AT3G12700 26.573 429 274 14 30 435 51 461 1.63e-30 123
MS.gene064526.t1 AT2G39710 29.288 379 210 16 89 429 67 425 2.21e-30 122
MS.gene064526.t1 AT1G65240 26.667 375 232 13 85 435 72 427 8.71e-30 121
MS.gene064526.t1 AT4G12920 27.013 385 242 14 61 434 31 387 2.55e-29 118
MS.gene064526.t1 AT3G02740 27.763 389 229 17 74 435 77 440 1.58e-27 114
MS.gene064526.t1 AT3G54400 27.400 427 257 16 30 435 31 425 2.42e-27 113
MS.gene064526.t1 AT3G02740 27.394 376 226 16 87 435 10 365 5.03e-27 112
MS.gene064526.t1 AT5G43100 23.990 396 260 15 63 437 45 420 5.24e-27 113
MS.gene064526.t1 AT3G59080 27.907 387 216 12 72 437 155 499 6.14e-27 113
MS.gene064526.t1 AT5G43100 23.990 396 260 15 63 437 45 420 9.87e-27 113
MS.gene064526.t1 AT5G02190 28.418 373 221 15 96 434 82 442 2.17e-26 111
MS.gene064526.t1 AT3G50050 26.829 410 248 18 55 437 55 439 2.95e-26 112
MS.gene064526.t1 AT5G45120 25.926 405 234 15 87 434 83 478 3.59e-26 110
MS.gene064526.t1 AT1G09750 27.123 365 233 13 85 434 102 448 1.24e-24 105
MS.gene064526.t1 AT5G37540 25.858 379 242 13 81 434 74 438 2.16e-24 105
MS.gene064526.t1 AT3G52500 29.500 400 214 20 85 435 88 468 2.92e-24 105
MS.gene064526.t1 AT1G66180 25.248 404 242 17 69 437 51 429 8.69e-24 103
MS.gene064526.t1 AT1G05840 24.147 381 240 12 85 435 78 439 1.92e-22 99.8
MS.gene064526.t1 AT5G22850 26.385 379 235 15 85 435 79 441 2.23e-22 99.8
MS.gene064526.t1 AT4G33490 23.773 387 231 17 85 437 58 414 1.65e-21 96.7
MS.gene064526.t1 AT1G44130 24.190 401 251 16 68 437 24 402 5.09e-21 95.1
MS.gene064526.t1 AT2G36670 24.138 377 240 14 87 435 34 392 5.79e-21 95.1
MS.gene064526.t1 AT2G36670 24.138 377 240 14 87 435 34 392 5.79e-21 95.1
MS.gene064526.t1 AT2G36670 24.274 379 241 14 85 435 98 458 6.90e-21 95.5
MS.gene064526.t1 AT1G08210 24.934 381 235 15 85 435 130 489 1.06e-20 95.1
MS.gene064526.t1 AT1G08210 24.934 381 235 15 85 435 116 475 1.28e-20 94.7
MS.gene064526.t1 AT2G36670 24.138 377 240 14 87 435 105 463 1.35e-20 94.4
MS.gene064526.t1 AT1G08210 24.934 381 235 15 85 435 82 441 1.52e-20 94.4
MS.gene064526.t1 AT3G51350 26.222 450 267 19 27 436 30 454 4.31e-20 93.2
MS.gene064526.t1 AT2G17760 27.123 365 220 16 92 434 109 449 6.78e-20 92.4
MS.gene064526.t1 AT1G77480 23.760 383 237 18 85 437 65 422 1.33e-19 90.9
MS.gene064526.t1 AT1G77480 23.760 383 237 18 85 437 65 422 2.41e-19 90.5
MS.gene064526.t1 AT5G07030 23.517 472 292 18 2 435 15 455 6.39e-19 89.0
MS.gene064526.t1 AT1G49050 21.138 369 235 14 104 436 49 397 1.10e-17 85.1
MS.gene064526.t1 AT1G49050 21.483 391 249 15 84 436 200 570 2.73e-17 84.7
MS.gene064526.t1 AT4G33490 29.947 187 109 7 85 263 55 227 2.91e-17 83.6
MS.gene064526.t1 AT3G42550 22.751 378 218 12 77 435 68 390 1.97e-16 81.3
MS.gene064526.t1 AT3G25700 29.121 182 108 6 39 207 39 212 2.57e-16 80.5
MS.gene064526.t1 AT1G08210 23.529 408 234 16 85 435 82 468 6.07e-16 80.5
MS.gene064526.t1 AT5G10080 27.708 397 217 22 11 362 7 378 7.68e-16 80.1
MS.gene064526.t1 AT1G49050 24.046 262 173 9 84 333 200 447 6.82e-14 73.9
MS.gene064526.t1 AT4G35880 22.554 368 236 15 93 437 113 454 7.26e-14 73.9
MS.gene064526.t1 AT4G35880 22.941 340 213 15 93 409 113 426 5.56e-11 64.7

Find 67 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTCCTAAAACTGTTAT+AGG 0.142168 6.2:+87982497 MS.gene064526:CDS
GGAGATGCCGCAATCGTTTC+TGG 0.188883 6.2:+87982704 MS.gene064526:CDS
TGTGAATATTCTATTAATTA+TGG 0.194092 6.2:+87982401 MS.gene064526:CDS
TCTAAGGATGTTTCCATCTT+TGG 0.213342 6.2:+87983124 MS.gene064526:CDS
GGGTCGAGTAAACTCAATTT+TGG 0.266363 6.2:+87982683 MS.gene064526:CDS
TTAAAATGTGCGGTCATATA+AGG 0.272062 6.2:-87983022 None:intergenic
ACAATACCATATGACTTAAT+TGG 0.286512 6.2:-87982209 None:intergenic
CTAAGGATGTTTCCATCTTT+GGG 0.301201 6.2:+87983125 MS.gene064526:CDS
GAATTCAGCAATGGTAGATT+TGG 0.327057 6.2:+87982919 MS.gene064526:CDS
TAGGAGATTACAGTTGATTC+AGG 0.336604 6.2:-87982146 None:intergenic
CACATCTCTAATAACACAAT+TGG 0.340665 6.2:+87982589 MS.gene064526:CDS
CATAGACTGAATTGTTGATT+AGG 0.346843 6.2:-87982965 None:intergenic
GATTAAACTTAGGAGGGGTT+TGG 0.366519 6.2:-87982289 None:intergenic
TTGTAACTTGATGACTTTGA+TGG 0.396449 6.2:-87982311 None:intergenic
AGCTCTGGTGTAGTTGGCCT+TGG 0.403335 6.2:+87982557 MS.gene064526:CDS
CCTAAAACTGTTATAGGATG+TGG 0.410701 6.2:+87982503 MS.gene064526:CDS
CGCGTAAGCTCTGGTGTAGT+TGG 0.418544 6.2:+87982551 MS.gene064526:CDS
ACACAATTGGGACCGTCAAT+TGG 0.426973 6.2:+87982602 MS.gene064526:CDS
GACAAAAGAGTAGAATTTCC+AGG 0.431627 6.2:+87982809 MS.gene064526:CDS
TGGTAAATTCTCTTATTGTT+TGG 0.432097 6.2:+87982625 MS.gene064526:CDS
ACCCCACCAATTAAGTCATA+TGG 0.439961 6.2:+87982203 MS.gene064526:CDS
GAAGTGACCGACACGATTGA+TGG 0.448925 6.2:-87982099 None:intergenic
TCTGGTGTAGTTGGCCTTGG+AGG 0.451277 6.2:+87982560 MS.gene064526:CDS
TCGTTTAAACGCGTAAGCTC+TGG 0.455409 6.2:+87982542 MS.gene064526:CDS
ACAATAGAAGCATTTAGTGT+TGG 0.456653 6.2:+87982785 MS.gene064526:CDS
TTCAATTTAGTATAAACATC+AGG 0.457412 6.2:-87982899 None:intergenic
GGTGTAGTTGGCCTTGGAGG+TGG 0.457901 6.2:+87982563 MS.gene064526:CDS
GGATGTGGAACTAACAATAC+AGG 0.467964 6.2:+87982518 MS.gene064526:CDS
GACTTTGATGGATTAAACTT+AGG 0.476937 6.2:-87982299 None:intergenic
TTGAGCTAAGCTCCCAAAGA+TGG 0.481930 6.2:-87983137 None:intergenic
AAAAGAAATACTAAAACAAA+AGG 0.486755 6.2:-87981958 None:intergenic
ATACAGGTAGTGACATTGTA+TGG 0.494388 6.2:+87982234 MS.gene064526:CDS
TCATATGGTATTGTCGATAC+AGG 0.494460 6.2:+87982218 MS.gene064526:CDS
TTTGATGGATTAAACTTAGG+AGG 0.495954 6.2:-87982296 None:intergenic
ACATCTCTAATAACACAATT+GGG 0.500209 6.2:+87982590 MS.gene064526:CDS
GAATTTCCAGGGTCCTCAAA+AGG 0.506670 6.2:+87982821 MS.gene064526:CDS
CTTAGCTCAACAAGATATGT+TGG 0.510416 6.2:+87983150 MS.gene064526:CDS
GCTCAACAAGATATGTTGGT+TGG 0.518263 6.2:+87983154 MS.gene064526:CDS
GAGAATTTACCACCAATTGA+CGG 0.523865 6.2:-87982614 None:intergenic
TCCTCAAAAGGTGTTGAAGA+AGG 0.536448 6.2:+87982833 MS.gene064526:CDS
TACCATATGACTTAATTGGT+GGG 0.543258 6.2:-87982205 None:intergenic
CAATTGTGTTATTAGAGATG+TGG 0.543750 6.2:-87982588 None:intergenic
AAATGAAATAGGACGGCCAT+TGG 0.543813 6.2:-87982480 None:intergenic
TTGATGGATTAAACTTAGGA+GGG 0.554944 6.2:-87982295 None:intergenic
AATTGTGTTATTAGAGATGT+GGG 0.556322 6.2:-87982587 None:intergenic
TACTAAATTGAATTCAGCAA+TGG 0.567220 6.2:+87982910 MS.gene064526:CDS
ATACCATATGACTTAATTGG+TGG 0.567621 6.2:-87982206 None:intergenic
CAATTGGGACCGTCAATTGG+TGG 0.579391 6.2:+87982605 MS.gene064526:CDS
TCAACTGTAATCTCCTACGA+GGG 0.579524 6.2:+87982152 MS.gene064526:CDS
TGATGGATTAAACTTAGGAG+GGG 0.584520 6.2:-87982294 None:intergenic
TGGTTGTAACATTGTTCACA+AGG 0.591014 6.2:-87982269 None:intergenic
GAGATGTGGGTCCACCTCCA+AGG 0.595636 6.2:-87982574 None:intergenic
ACCATATGACTTAATTGGTG+GGG 0.597911 6.2:-87982204 None:intergenic
TATATCATGAGCTATTCAGT+TGG 0.598495 6.2:+87982179 MS.gene064526:CDS
CTTCAACCCTCATTACACTT+CGG 0.599430 6.2:-87983224 None:intergenic
ATCCGCACCTTTAAAATGTG+CGG 0.612439 6.2:-87983032 None:intergenic
ACAAAAGAGTAGAATTTCCA+GGG 0.643770 6.2:+87982810 MS.gene064526:CDS
CATGATATAATCACCCTCGT+AGG 0.657331 6.2:-87982165 None:intergenic
AATTTAGTATAAACATCAGG+TGG 0.660177 6.2:-87982896 None:intergenic
GGCAATCAATCACATTCACA+AGG 0.662936 6.2:+87982422 MS.gene064526:CDS
CTTACATTAGAATCTACCAA+TGG 0.676774 6.2:+87982464 MS.gene064526:CDS
CGTCGTTCCATCAATCGTGT+CGG 0.677525 6.2:+87982092 MS.gene064526:CDS
GATGTGGAACTAACAATACA+GGG 0.681631 6.2:+87982519 MS.gene064526:CDS
GTACTGCATTGACAACATGT+TGG 0.687626 6.2:-87982070 None:intergenic
GATGGAAACATCCTTAGACG+AGG 0.728844 6.2:-87983119 None:intergenic
ATCAACTGTAATCTCCTACG+AGG 0.740288 6.2:+87982151 MS.gene064526:CDS
ATTGTGACCAGAAACGATTG+CGG 0.753392 6.2:-87982711 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAAAAAATATGTCAAT+GGG + chr6.2:87982662-87982681 MS.gene064526:CDS 10.0%
!! TATATTAAAAAATATGTCAA+TGG + chr6.2:87982661-87982680 MS.gene064526:CDS 10.0%
!! AAAAGAAATACTAAAACAAA+AGG - chr6.2:87981961-87981980 None:intergenic 15.0%
!! TATTAAAAAATATGTCAATG+GGG + chr6.2:87982663-87982682 MS.gene064526:CDS 15.0%
!! TGTGAATATTCTATTAATTA+TGG + chr6.2:87982401-87982420 MS.gene064526:CDS 15.0%
!! AACAAAAGGAAAAGAAAAAA+AGG - chr6.2:87981947-87981966 None:intergenic 20.0%
!! ACAAAAGGAAAAGAAAAAAA+GGG - chr6.2:87981946-87981965 None:intergenic 20.0%
!! TCAAATAGTAGAAAAATGAA+GGG - chr6.2:87982766-87982785 None:intergenic 20.0%
!! TTCAATTTAGTATAAACATC+AGG - chr6.2:87982902-87982921 None:intergenic 20.0%
! AAAGAAAAAAAGGGTAAGAA+AGG - chr6.2:87981937-87981956 None:intergenic 25.0%
! AATTTAGTATAAACATCAGG+TGG - chr6.2:87982899-87982918 None:intergenic 25.0%
! ACAATACCATATGACTTAAT+TGG - chr6.2:87982212-87982231 None:intergenic 25.0%
! ACATCTCTAATAACACAATT+GGG + chr6.2:87982590-87982609 MS.gene064526:CDS 25.0%
! GTCAAATAGTAGAAAAATGA+AGG - chr6.2:87982767-87982786 None:intergenic 25.0%
! TACTAAATTGAATTCAGCAA+TGG + chr6.2:87982910-87982929 MS.gene064526:CDS 25.0%
! TCATTTCCTAAAACTGTTAT+AGG + chr6.2:87982497-87982516 MS.gene064526:CDS 25.0%
! TGGTAAATTCTCTTATTGTT+TGG + chr6.2:87982625-87982644 MS.gene064526:CDS 25.0%
!! AATTGTGTTATTAGAGATGT+GGG - chr6.2:87982590-87982609 None:intergenic 25.0%
!!! ACAGTTTTAGGAAATGAAAT+AGG - chr6.2:87982494-87982513 None:intergenic 25.0%
!!! TGTTGGTATTTATTTTGTGT+AGG - chr6.2:87982056-87982075 None:intergenic 25.0%
!!! TTTCTTTTTCTCAAGTTGTA+AGG + chr6.2:87981973-87981992 MS.gene064526:CDS 25.0%
!!! TTTTAGGAAATGAAATAGGA+CGG - chr6.2:87982490-87982509 None:intergenic 25.0%
ACAAAAGAGTAGAATTTCCA+GGG + chr6.2:87982810-87982829 MS.gene064526:CDS 30.0%
ATACCATATGACTTAATTGG+TGG - chr6.2:87982209-87982228 None:intergenic 30.0%
CACATCTCTAATAACACAAT+TGG + chr6.2:87982589-87982608 MS.gene064526:CDS 30.0%
CTTACATTAGAATCTACCAA+TGG + chr6.2:87982464-87982483 MS.gene064526:CDS 30.0%
TACCATATGACTTAATTGGT+GGG - chr6.2:87982208-87982227 None:intergenic 30.0%
TATATCATGAGCTATTCAGT+TGG + chr6.2:87982179-87982198 MS.gene064526:CDS 30.0%
TTAAAATGTGCGGTCATATA+AGG - chr6.2:87983025-87983044 None:intergenic 30.0%
! CAATTGTGTTATTAGAGATG+TGG - chr6.2:87982591-87982610 None:intergenic 30.0%
! CATAGACTGAATTGTTGATT+AGG - chr6.2:87982968-87982987 None:intergenic 30.0%
! TATATGACCGCACATTTTAA+AGG + chr6.2:87983025-87983044 MS.gene064526:CDS 30.0%
! TTGTAACTTGATGACTTTGA+TGG - chr6.2:87982314-87982333 None:intergenic 30.0%
! TTTTGTGTAGGCTTGTAAAA+TGG - chr6.2:87982044-87982063 None:intergenic 30.0%
!! ACAATAGAAGCATTTAGTGT+TGG + chr6.2:87982785-87982804 MS.gene064526:CDS 30.0%
!! GACTTTGATGGATTAAACTT+AGG - chr6.2:87982302-87982321 None:intergenic 30.0%
!! TTGATGGATTAAACTTAGGA+GGG - chr6.2:87982298-87982317 None:intergenic 30.0%
!! TTTGATGGATTAAACTTAGG+AGG - chr6.2:87982299-87982318 None:intergenic 30.0%
!! TTTTCTCAAGTTGTAAGGAA+TGG + chr6.2:87981978-87981997 MS.gene064526:CDS 30.0%
!!! AAGACACAGTTTTTTGTTGA+AGG - chr6.2:87983186-87983205 None:intergenic 30.0%
!!! GAAGGGTCTTTTTTAACTAT+AGG - chr6.2:87982749-87982768 None:intergenic 30.0%
ACCATATGACTTAATTGGTG+GGG - chr6.2:87982207-87982226 None:intergenic 35.0%
ATACAGGTAGTGACATTGTA+TGG + chr6.2:87982234-87982253 MS.gene064526:CDS 35.0%
CCTAAAACTGTTATAGGATG+TGG + chr6.2:87982503-87982522 MS.gene064526:CDS 35.0%
CTAAGGATGTTTCCATCTTT+GGG + chr6.2:87983125-87983144 MS.gene064526:CDS 35.0%
CTTAGCTCAACAAGATATGT+TGG + chr6.2:87983150-87983169 MS.gene064526:CDS 35.0%
GAATTCAGCAATGGTAGATT+TGG + chr6.2:87982919-87982938 MS.gene064526:CDS 35.0%
GACAAAAGAGTAGAATTTCC+AGG + chr6.2:87982809-87982828 MS.gene064526:CDS 35.0%
GAGAATTTACCACCAATTGA+CGG - chr6.2:87982617-87982636 None:intergenic 35.0%
GATGTGGAACTAACAATACA+GGG + chr6.2:87982519-87982538 MS.gene064526:CDS 35.0%
TCATATGGTATTGTCGATAC+AGG + chr6.2:87982218-87982237 MS.gene064526:CDS 35.0%
TCTAAGGATGTTTCCATCTT+TGG + chr6.2:87983124-87983143 MS.gene064526:CDS 35.0%
TGGTTGTAACATTGTTCACA+AGG - chr6.2:87982272-87982291 None:intergenic 35.0%
! CCACATCCTATAACAGTTTT+AGG - chr6.2:87982506-87982525 None:intergenic 35.0%
! TAGGAGATTACAGTTGATTC+AGG - chr6.2:87982149-87982168 None:intergenic 35.0%
!! ATGGTGATTTTAACGAGTCA+CGG - chr6.2:87982025-87982044 None:intergenic 35.0%
!! TGATGGATTAAACTTAGGAG+GGG - chr6.2:87982297-87982316 None:intergenic 35.0%
AAATGAAATAGGACGGCCAT+TGG - chr6.2:87982483-87982502 None:intergenic 40.0%
ACCCCACCAATTAAGTCATA+TGG + chr6.2:87982203-87982222 MS.gene064526:CDS 40.0%
ATCAACTGTAATCTCCTACG+AGG + chr6.2:87982151-87982170 MS.gene064526:CDS 40.0%
ATTGTGACCAGAAACGATTG+CGG - chr6.2:87982714-87982733 None:intergenic 40.0%
CATGATATAATCACCCTCGT+AGG - chr6.2:87982168-87982187 None:intergenic 40.0%
GATTAAACTTAGGAGGGGTT+TGG - chr6.2:87982292-87982311 None:intergenic 40.0%
GCTCAACAAGATATGTTGGT+TGG + chr6.2:87983154-87983173 MS.gene064526:CDS 40.0%
GGATGTGGAACTAACAATAC+AGG + chr6.2:87982518-87982537 MS.gene064526:CDS 40.0%
GGCAATCAATCACATTCACA+AGG + chr6.2:87982422-87982441 MS.gene064526:CDS 40.0%
TCAACTGTAATCTCCTACGA+GGG + chr6.2:87982152-87982171 MS.gene064526:CDS 40.0%
! ACCTTCTTCAACACCTTTTG+AGG - chr6.2:87982837-87982856 None:intergenic 40.0%
! GGGTCGAGTAAACTCAATTT+TGG + chr6.2:87982683-87982702 MS.gene064526:CDS 40.0%
! GTACTGCATTGACAACATGT+TGG - chr6.2:87982073-87982092 None:intergenic 40.0%
!! ATCCGCACCTTTAAAATGTG+CGG - chr6.2:87983035-87983054 None:intergenic 40.0%
!! TCCTCAAAAGGTGTTGAAGA+AGG + chr6.2:87982833-87982852 MS.gene064526:CDS 40.0%
ACACAATTGGGACCGTCAAT+TGG + chr6.2:87982602-87982621 MS.gene064526:CDS 45.0%
GAATTTCCAGGGTCCTCAAA+AGG + chr6.2:87982821-87982840 MS.gene064526:CDS 45.0%
GATGGAAACATCCTTAGACG+AGG - chr6.2:87983122-87983141 None:intergenic 45.0%
TCGTTTAAACGCGTAAGCTC+TGG + chr6.2:87982542-87982561 MS.gene064526:CDS 45.0%
TTGAGCTAAGCTCCCAAAGA+TGG - chr6.2:87983140-87983159 None:intergenic 45.0%
!! CTTTGCTTTTGCCTCGTCTA+AGG + chr6.2:87983108-87983127 MS.gene064526:CDS 45.0%
!! GACCGCACATTTTAAAGGTG+CGG + chr6.2:87983030-87983049 MS.gene064526:CDS 45.0%
CAATTGGGACCGTCAATTGG+TGG + chr6.2:87982605-87982624 MS.gene064526:CDS 50.0%
CGTCGTTCCATCAATCGTGT+CGG + chr6.2:87982092-87982111 MS.gene064526:CDS 50.0%
GAAGTGACCGACACGATTGA+TGG - chr6.2:87982102-87982121 None:intergenic 50.0%
!! TCAACACCTTTTGAGGACCC+TGG - chr6.2:87982830-87982849 None:intergenic 50.0%
GGAGATGCCGCAATCGTTTC+TGG + chr6.2:87982704-87982723 MS.gene064526:CDS 55.0%
! AGCTCTGGTGTAGTTGGCCT+TGG + chr6.2:87982557-87982576 MS.gene064526:CDS 55.0%
! CGCGTAAGCTCTGGTGTAGT+TGG + chr6.2:87982551-87982570 MS.gene064526:CDS 55.0%
!! TCTGGTGTAGTTGGCCTTGG+AGG + chr6.2:87982560-87982579 MS.gene064526:CDS 55.0%
GAGATGTGGGTCCACCTCCA+AGG - chr6.2:87982577-87982596 None:intergenic 60.0%
!! GGTGTAGTTGGCCTTGGAGG+TGG + chr6.2:87982563-87982582 MS.gene064526:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 87981921 87983234 87981921 ID=MS.gene064526
chr6.2 mRNA 87981921 87983234 87981921 ID=MS.gene064526.t1;Parent=MS.gene064526
chr6.2 exon 87981921 87983234 87981921 ID=MS.gene064526.t1.exon1;Parent=MS.gene064526.t1
chr6.2 CDS 87981921 87983234 87981921 ID=cds.MS.gene064526.t1;Parent=MS.gene064526.t1
Gene Sequence

>MS.gene064526

ATGAACACACTTTCCTTTCTTACCCTTTTTTTCTTTTCCTTTTGTTTTAGTATTTCTTTTTCTCAAGTTGTAAGGAATGGTTTTAGTGTTGAACTTATACACCGTGACTCGTTAAAATCACCATTTTACAAGCCTACACAAAATAAATACCAACATGTTGTCAATGCAGTACGTCGTTCCATCAATCGTGTCGGTCACTTCTACAAGAATTCTCTCGCAAGCACACCTGAATCAACTGTAATCTCCTACGAGGGTGATTATATCATGAGCTATTCAGTTGGTACCCCACCAATTAAGTCATATGGTATTGTCGATACAGGTAGTGACATTGTATGGCTACAATGCGAGCCTTGTGAACAATGTTACAACCAAACCCCTCCTAAGTTTAATCCATCAAAGTCATCAAGTTACAAAAACATTTCTTGTTCGTCTAAACTATGTCAATCTGTGAAAGATATCTCTTGCAATGACAAAAAAAATTGTGAATATTCTATTAATTATGGCAATCAATCACATTCACAAGGAGATCTTAGTGTTGAGACTCTTACATTAGAATCTACCAATGGCCGTCCTATTTCATTTCCTAAAACTGTTATAGGATGTGGAACTAACAATACAGGGTCGTTTAAACGCGTAAGCTCTGGTGTAGTTGGCCTTGGAGGTGGACCCACATCTCTAATAACACAATTGGGACCGTCAATTGGTGGTAAATTCTCTTATTGTTTGGTGCGAATGTCAATTATATTAAAAAATATGTCAATGGGGTCGAGTAAACTCAATTTTGGAGATGCCGCAATCGTTTCTGGTCACAATGTATTGTCAACTCCTATAGTTAAAAAAGACCCTTCATTTTTCTACTATTTGACAATAGAAGCATTTAGTGTTGGAGACAAAAGAGTAGAATTTCCAGGGTCCTCAAAAGGTGTTGAAGAAGGTAACATCATAATTGATTCAAGTACAATTGTGACATTTGTACCACCTGATGTTTATACTAAATTGAATTCAGCAATGGTAGATTTGGTGACACTAGAACGTGTTGACGATCCTAATCAACAATTCAGTCTATGTTATAATTCGTCATCAGATGAAGAATATGATTTTCCTTATATGACCGCACATTTTAAAGGTGCGGATATTTTGTTGTATGCTACTAATACATTTATCGAAGTTGCTAGAGATGTTCTTTGCTTTGCTTTTGCCTCGTCTAAGGATGTTTCCATCTTTGGGAGCTTAGCTCAACAAGATATGTTGGTTGGTTATGACCTTCAACAAAAAACTGTGTCTTTCAAGTCTGTAGATTGTACCGAAGTGTAA
Protein sequence

>MS.gene064526.t1

MNTLSFLTLFFFSFCFSISFSQVVRNGFSVELIHRDSLKSPFYKPTQNKYQHVVNAVRRSINRVGHFYKNSLASTPESTVISYEGDYIMSYSVGTPPIKSYGIVDTGSDIVWLQCEPCEQCYNQTPPKFNPSKSSSYKNISCSSKLCQSVKDISCNDKKNCEYSINYGNQSHSQGDLSVETLTLESTNGRPISFPKTVIGCGTNNTGSFKRVSSGVVGLGGGPTSLITQLGPSIGGKFSYCLVRMSIILKNMSMGSSKLNFGDAAIVSGHNVLSTPIVKKDPSFFYYLTIEAFSVGDKRVEFPGSSKGVEEGNIIIDSSTIVTFVPPDVYTKLNSAMVDLVTLERVDDPNQQFSLCYNSSSDEEYDFPYMTAHFKGADILLYATNTFIEVARDVLCFAFASSKDVSIFGSLAQQDMLVGYDLQQKTVSFKSVDCTEV