AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064659


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064659.t1 MTR_3g111780 94.366 284 15 1 1 284 1 283 2.06e-180 498
MS.gene064659.t1 MTR_8g103520 51.389 72 35 0 193 264 218 289 1.50e-20 89.4
MS.gene064659.t1 MTR_0907s0020 54.545 66 30 0 199 264 102 167 1.23e-19 84.7
MS.gene064659.t1 MTR_5g075210 43.000 100 57 0 164 263 245 344 4.89e-19 85.9
MS.gene064659.t1 MTR_7g072875 32.468 154 80 2 129 260 129 280 2.01e-18 83.6
MS.gene064659.t1 MTR_7g105790 53.623 69 32 0 202 270 113 181 6.05e-18 80.5
MS.gene064659.t1 MTR_4g052040 53.846 65 30 0 200 264 100 164 1.84e-17 78.6
MS.gene064659.t1 MTR_7g072630 26.829 287 155 8 27 260 58 342 4.44e-17 80.5
MS.gene064659.t1 MTR_5g079710 50.725 69 34 0 196 264 88 156 5.36e-17 79.0
MS.gene064659.t1 MTR_8g087530 47.692 65 34 0 199 263 258 322 6.48e-16 77.0
MS.gene064659.t1 MTR_2g018030 46.774 62 33 0 199 260 277 338 9.53e-16 76.6
MS.gene064659.t1 MTR_4g028930 50.820 61 30 0 200 260 315 375 6.92e-15 74.3
MS.gene064659.t1 MTR_4g052060 51.667 60 29 0 205 264 99 158 1.98e-13 67.8
MS.gene064659.t1 MTR_1g111640 45.161 62 34 0 198 259 136 197 3.74e-11 62.4
MS.gene064659.t1 MTR_4g132740 37.500 88 44 1 178 265 71 147 6.18e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064659.t1 AT5G19650 62.121 66 25 0 199 264 154 219 7.03e-23 94.4
MS.gene064659.t1 AT2G18500 61.538 65 25 0 199 263 226 290 2.81e-20 89.0
MS.gene064659.t1 AT2G30400 50.000 70 35 0 194 263 247 316 1.65e-18 84.3
MS.gene064659.t1 AT3G52525 56.452 62 27 0 200 261 67 128 6.94e-18 79.3
MS.gene064659.t1 AT5G58360 53.731 67 31 0 197 263 222 288 2.07e-17 80.9
MS.gene064659.t1 AT2G36026 55.000 60 27 0 205 264 92 151 5.15e-17 77.4
MS.gene064659.t1 AT1G06920 33.010 103 64 1 167 264 206 308 4.60e-16 77.4
MS.gene064659.t1 AT5G01840 48.529 68 35 0 203 270 202 269 4.30e-15 73.9
MS.gene064659.t1 AT5G22240 44.118 68 38 0 196 263 93 160 1.19e-13 68.6
MS.gene064659.t1 AT4G18830 45.902 61 33 0 199 259 282 342 8.61e-12 65.1

Find 66 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGCAGGGCCTGGATTCC+CGG 0.272464 3.4:+96928842 None:intergenic
CTCAAATTATAGTGATAAAA+AGG 0.274257 3.4:-96928538 MS.gene064659:CDS
GGTCCATTCTTCTAGTACTA+TGG 0.311039 3.4:-96928583 MS.gene064659:CDS
GGGGAAAGAGGAAAGGTTAT+TGG 0.328740 3.4:+96928863 None:intergenic
TGCGAGCTTTCATGGAGATT+TGG 0.332540 3.4:-96928161 MS.gene064659:CDS
AGTAATTGTGCGAGCTTTCA+TGG 0.344864 3.4:-96928169 MS.gene064659:CDS
GACACGTTGCATTGGTGTTC+CGG 0.349518 3.4:+96928753 None:intergenic
CACCGGAGAAAGACGGTATA+TGG 0.359937 3.4:+96928824 None:intergenic
TCCTCTTTCCCCGGGAATCC+AGG 0.367021 3.4:-96928852 MS.gene064659:CDS
TTTGGCAACAAATGTTCGTT+TGG 0.381425 3.4:-96928143 MS.gene064659:CDS
GGCGACGAAGCAGGAAGGTT+TGG 0.382600 3.4:+96928774 None:intergenic
TTATCACTATAATTTGAGAT+AGG 0.385663 3.4:+96928543 None:intergenic
ATACCCTATGTCATATGCTT+TGG 0.387292 3.4:+96928797 None:intergenic
GGTAAAAGAAAGCTTTGCTT+TGG 0.392617 3.4:-96928331 MS.gene064659:CDS
AGTGAGGTTTCAAAGTATTA+CGG 0.405418 3.4:-96928454 MS.gene064659:CDS
ATTTAACTAGCTCGAATAGA+AGG 0.424510 3.4:-96928506 MS.gene064659:CDS
ACTTGACATAACCTTCGTTT+CGG 0.428668 3.4:+96928368 None:intergenic
GATGTCCGAGGCCAAGGACT+TGG 0.437700 3.4:-96928241 MS.gene064659:CDS
ATGGCAGGGCCTGGATTCCC+GGG 0.439698 3.4:+96928843 None:intergenic
GTTAGAGATGATAGAAGAGA+TGG 0.441612 3.4:-96928265 MS.gene064659:CDS
TTCTTGCTTAGCAAGATTCT+CGG 0.449560 3.4:-96928616 MS.gene064659:CDS
AATAACCTTTCCTCTTTCCC+CGG 0.452894 3.4:-96928861 MS.gene064659:CDS
GAAGGTTATGTCAAGTAGAA+TGG 0.463702 3.4:-96928361 MS.gene064659:CDS
GAAGGTTATGTCAAGTAGAA+TGG 0.463702 3.4:-96928409 MS.gene064659:CDS
CTATGGATAGTACTAAAGTT+AGG 0.470027 3.4:-96928566 MS.gene064659:CDS
CAATAGTTGCTCCAAGTCCT+TGG 0.478606 3.4:+96928230 None:intergenic
TCAAACAAATGTTCAAACTA+AGG 0.481359 3.4:-96928097 MS.gene064659:CDS
AGACGGTATATGGCAGGGCC+TGG 0.484707 3.4:+96928834 None:intergenic
ACTAGAAGAATGGACCTTCT+CGG 0.488884 3.4:+96928590 None:intergenic
GCCTGGATTCCCGGGGAAAG+AGG 0.494617 3.4:+96928851 None:intergenic
TATCCATAGTACTAGAAGAA+TGG 0.495158 3.4:+96928580 None:intergenic
AGAAACTCACTTCTTCTTGA+GGG 0.501729 3.4:+96928678 None:intergenic
TGGTGTTCCGGCGACGAAGC+AGG 0.502249 3.4:+96928765 None:intergenic
GATGGAGATGTCCGAGGCCA+AGG 0.503048 3.4:-96928247 MS.gene064659:CDS
TGCCATATACCGTCTTTCTC+CGG 0.503136 3.4:-96928826 MS.gene064659:CDS
GAGCGACACATTTGAAAAGA+AGG 0.503682 3.4:+96928885 None:intergenic
GTCAAGTAGAATGGAGGAGA+AGG 0.514162 3.4:-96928352 MS.gene064659:CDS
AAACCTTCCTGCTTCGTCGC+CGG 0.514759 3.4:-96928772 MS.gene064659:CDS
TTGCTCCAAGTCCTTGGCCT+CGG 0.515968 3.4:+96928236 None:intergenic
AGCGACACATTTGAAAAGAA+GGG 0.522344 3.4:+96928886 None:intergenic
GGAGAAAGACGGTATATGGC+AGG 0.531009 3.4:+96928828 None:intergenic
TAGAAACTCACTTCTTCTTG+AGG 0.531127 3.4:+96928677 None:intergenic
ATAACCTTTCCTCTTTCCCC+GGG 0.540129 3.4:-96928860 MS.gene064659:CDS
TCATATGCTTTGGAGTTCAC+CGG 0.541099 3.4:+96928807 None:intergenic
CTAAATTCTAGAGATTATCA+TGG 0.544872 3.4:-96928192 MS.gene064659:CDS
GATTTCTTGAAGTCTTCGTA+AGG 0.562850 3.4:+96928291 None:intergenic
AATTGATAAAATCAATGAAG+AGG 0.573253 3.4:-96928655 MS.gene064659:CDS
AAGATTCTCGGAAGCCGAGA+AGG 0.578226 3.4:-96928604 MS.gene064659:CDS
AACTCCAAAGCATATGACAT+AGG 0.582440 3.4:-96928801 MS.gene064659:CDS
TCTTTGATGACACGTTGCAT+TGG 0.597146 3.4:+96928745 None:intergenic
TCTTCTTGAGGGAACGTCGG+AGG 0.601171 3.4:+96928689 None:intergenic
ACTCCAAAGCATATGACATA+GGG 0.601832 3.4:-96928800 MS.gene064659:CDS
TCGAAGAGGAACCGAAACGA+AGG 0.602284 3.4:-96928379 MS.gene064659:CDS
TGAAGAATGTTGAGAGAGTG+AGG 0.603540 3.4:-96928470 MS.gene064659:CDS
ATTAAGAAAGTGAAATCCAA+AGG 0.608408 3.4:-96928723 MS.gene064659:CDS
TGGAGTTCACCGGAGAAAGA+CGG 0.608559 3.4:+96928817 None:intergenic
GTTCCGGCGACGAAGCAGGA+AGG 0.630275 3.4:+96928769 None:intergenic
GAGAAAGACGGTATATGGCA+GGG 0.639308 3.4:+96928829 None:intergenic
GATTCCCGGGGAAAGAGGAA+AGG 0.642563 3.4:+96928856 None:intergenic
ACTTCTTCTTGAGGGAACGT+CGG 0.649804 3.4:+96928686 None:intergenic
AGTATTACGGAGAATCAAAG+AGG 0.650942 3.4:-96928441 MS.gene064659:CDS
TGGCAGGGCCTGGATTCCCG+GGG 0.653477 3.4:+96928844 None:intergenic
GGTTATGTCAAGTAGAATGG+AGG 0.656874 3.4:-96928358 MS.gene064659:CDS
AGAATGGAAGAGAATCGAAG+AGG 0.657497 3.4:-96928393 MS.gene064659:CDS
TCAAAGAGGAAAAGAAACGA+AGG 0.672215 3.4:-96928427 MS.gene064659:CDS
AGAAGAGATGGAGATGTCCG+AGG 0.675634 3.4:-96928253 MS.gene064659:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGATAAAATCAATGAAG+AGG - chr3.4:96928346-96928365 MS.gene064659:CDS 20.0%
!! CTCAAATTATAGTGATAAAA+AGG - chr3.4:96928463-96928482 MS.gene064659:CDS 20.0%
!!! TTATCACTATAATTTGAGAT+AGG + chr3.4:96928461-96928480 None:intergenic 20.0%
! ATTAAGAAAGTGAAATCCAA+AGG - chr3.4:96928278-96928297 MS.gene064659:CDS 25.0%
! TCAAACAAATGTTCAAACTA+AGG - chr3.4:96928904-96928923 MS.gene064659:CDS 25.0%
!! CTAAATTCTAGAGATTATCA+TGG - chr3.4:96928809-96928828 MS.gene064659:CDS 25.0%
!!! TGAAGATTTTTTACTGACTT+AGG + chr3.4:96928887-96928906 None:intergenic 25.0%
ATTTAACTAGCTCGAATAGA+AGG - chr3.4:96928495-96928514 MS.gene064659:CDS 30.0%
CTATGGATAGTACTAAAGTT+AGG - chr3.4:96928435-96928454 MS.gene064659:CDS 30.0%
TATCCATAGTACTAGAAGAA+TGG + chr3.4:96928424-96928443 None:intergenic 30.0%
! AGTGAGGTTTCAAAGTATTA+CGG - chr3.4:96928547-96928566 MS.gene064659:CDS 30.0%
AACTCCAAAGCATATGACAT+AGG - chr3.4:96928200-96928219 MS.gene064659:CDS 35.0%
ACTCCAAAGCATATGACATA+GGG - chr3.4:96928201-96928220 MS.gene064659:CDS 35.0%
ACTTGACATAACCTTCGTTT+CGG + chr3.4:96928588-96928607 None:intergenic 35.0%
AGAAACTCACTTCTTCTTGA+GGG + chr3.4:96928326-96928345 None:intergenic 35.0%
AGCGACACATTTGAAAAGAA+GGG + chr3.4:96928118-96928137 None:intergenic 35.0%
AGTATTACGGAGAATCAAAG+AGG - chr3.4:96928560-96928579 MS.gene064659:CDS 35.0%
ATACCCTATGTCATATGCTT+TGG + chr3.4:96928207-96928226 None:intergenic 35.0%
GAAGGTTATGTCAAGTAGAA+TGG - chr3.4:96928592-96928611 MS.gene064659:CDS 35.0%
GATTTCTTGAAGTCTTCGTA+AGG + chr3.4:96928713-96928732 None:intergenic 35.0%
GGTAAAAGAAAGCTTTGCTT+TGG - chr3.4:96928670-96928689 MS.gene064659:CDS 35.0%
GTTAGAGATGATAGAAGAGA+TGG - chr3.4:96928736-96928755 MS.gene064659:CDS 35.0%
TAGAAACTCACTTCTTCTTG+AGG + chr3.4:96928327-96928346 None:intergenic 35.0%
TCAAAGAGGAAAAGAAACGA+AGG - chr3.4:96928574-96928593 MS.gene064659:CDS 35.0%
TGCTTTGGTGAAAAAGTCAA+AGG - chr3.4:96928685-96928704 MS.gene064659:CDS 35.0%
TTCTTGCTTAGCAAGATTCT+CGG - chr3.4:96928385-96928404 MS.gene064659:CDS 35.0%
TTTGGCAACAAATGTTCGTT+TGG - chr3.4:96928858-96928877 MS.gene064659:CDS 35.0%
!!! GCAACTATTGCAGTGTTTTT+TGG - chr3.4:96928784-96928803 MS.gene064659:CDS 35.0%
AATAACCTTTCCTCTTTCCC+CGG - chr3.4:96928140-96928159 MS.gene064659:CDS 40.0%
ACTAGAAGAATGGACCTTCT+CGG + chr3.4:96928414-96928433 None:intergenic 40.0%
AGAATGGAAGAGAATCGAAG+AGG - chr3.4:96928608-96928627 MS.gene064659:CDS 40.0%
AGTAATTGTGCGAGCTTTCA+TGG - chr3.4:96928832-96928851 MS.gene064659:CDS 40.0%
GAGCGACACATTTGAAAAGA+AGG + chr3.4:96928119-96928138 None:intergenic 40.0%
GGTCCATTCTTCTAGTACTA+TGG - chr3.4:96928418-96928437 MS.gene064659:CDS 40.0%
GGTTATGTCAAGTAGAATGG+AGG - chr3.4:96928595-96928614 MS.gene064659:CDS 40.0%
TGAAGAATGTTGAGAGAGTG+AGG - chr3.4:96928531-96928550 MS.gene064659:CDS 40.0%
! TCTTTGATGACACGTTGCAT+TGG + chr3.4:96928259-96928278 None:intergenic 40.0%
!! TCATATGCTTTGGAGTTCAC+CGG + chr3.4:96928197-96928216 None:intergenic 40.0%
ACTTCTTCTTGAGGGAACGT+CGG + chr3.4:96928318-96928337 None:intergenic 45.0%
ATAACCTTTCCTCTTTCCCC+GGG - chr3.4:96928141-96928160 MS.gene064659:CDS 45.0%
CAATAGTTGCTCCAAGTCCT+TGG + chr3.4:96928774-96928793 None:intergenic 45.0%
GAGAAAGACGGTATATGGCA+GGG + chr3.4:96928175-96928194 None:intergenic 45.0%
GGGGAAAGAGGAAAGGTTAT+TGG + chr3.4:96928141-96928160 None:intergenic 45.0%
GTCAAGTAGAATGGAGGAGA+AGG - chr3.4:96928649-96928668 MS.gene064659:CDS 45.0%
TGCCATATACCGTCTTTCTC+CGG - chr3.4:96928175-96928194 MS.gene064659:CDS 45.0%
TGCGAGCTTTCATGGAGATT+TGG - chr3.4:96928840-96928859 MS.gene064659:CDS 45.0%
AAGATTCTCGGAAGCCGAGA+AGG - chr3.4:96928397-96928416 MS.gene064659:CDS 50.0%
AGAAGAGATGGAGATGTCCG+AGG - chr3.4:96928748-96928767 MS.gene064659:CDS 50.0%
CACCGGAGAAAGACGGTATA+TGG + chr3.4:96928180-96928199 None:intergenic 50.0%
GGAGAAAGACGGTATATGGC+AGG + chr3.4:96928176-96928195 None:intergenic 50.0%
TCGAAGAGGAACCGAAACGA+AGG - chr3.4:96928622-96928641 MS.gene064659:CDS 50.0%
TGGAGTTCACCGGAGAAAGA+CGG + chr3.4:96928187-96928206 None:intergenic 50.0%
! GACACGTTGCATTGGTGTTC+CGG + chr3.4:96928251-96928270 None:intergenic 50.0%
AAACCTTCCTGCTTCGTCGC+CGG - chr3.4:96928229-96928248 MS.gene064659:CDS 55.0%
GATTCCCGGGGAAAGAGGAA+AGG + chr3.4:96928148-96928167 None:intergenic 55.0%
TCTTCTTGAGGGAACGTCGG+AGG + chr3.4:96928315-96928334 None:intergenic 55.0%
TTGCTCCAAGTCCTTGGCCT+CGG + chr3.4:96928768-96928787 None:intergenic 55.0%
!!! GAACGTCGGAGGTGCTTTTG+CGG + chr3.4:96928304-96928323 None:intergenic 55.0%
AGACGGTATATGGCAGGGCC+TGG + chr3.4:96928170-96928189 None:intergenic 60.0%
GATGGAGATGTCCGAGGCCA+AGG - chr3.4:96928754-96928773 MS.gene064659:CDS 60.0%
GATGTCCGAGGCCAAGGACT+TGG - chr3.4:96928760-96928779 MS.gene064659:CDS 60.0%
GGCGACGAAGCAGGAAGGTT+TGG + chr3.4:96928230-96928249 None:intergenic 60.0%
TATGGCAGGGCCTGGATTCC+CGG + chr3.4:96928162-96928181 None:intergenic 60.0%
TCCTCTTTCCCCGGGAATCC+AGG - chr3.4:96928149-96928168 MS.gene064659:CDS 60.0%
!!! GGAGGTGCTTTTGCGGCCTT+TGG + chr3.4:96928297-96928316 None:intergenic 60.0%
ATGGCAGGGCCTGGATTCCC+GGG + chr3.4:96928161-96928180 None:intergenic 65.0%
GCCTGGATTCCCGGGGAAAG+AGG + chr3.4:96928153-96928172 None:intergenic 65.0%
GTTCCGGCGACGAAGCAGGA+AGG + chr3.4:96928235-96928254 None:intergenic 65.0%
TGGTGTTCCGGCGACGAAGC+AGG + chr3.4:96928239-96928258 None:intergenic 65.0%
TGGCAGGGCCTGGATTCCCG+GGG + chr3.4:96928160-96928179 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 96928083 96928940 96928083 ID=MS.gene064659
chr3.4 mRNA 96928083 96928940 96928083 ID=MS.gene064659.t1;Parent=MS.gene064659
chr3.4 exon 96928083 96928940 96928083 ID=MS.gene064659.t1.exon1;Parent=MS.gene064659.t1
chr3.4 CDS 96928083 96928940 96928083 ID=cds.MS.gene064659.t1;Parent=MS.gene064659.t1
Gene Sequence

>MS.gene064659

ATGTCTAAACGCTTTAAGATGAAATTTACAATCCCTTCTTTTCAAATGTGTCGCTCCAATAACCTTTCCTCTTTCCCCGGGAATCCAGGCCCTGCCATATACCGTCTTTCTCCGGTGAACTCCAAAGCATATGACATAGGGTATCCAAACCTTCCTGCTTCGTCGCCGGAACACCAATGCAACGTGTCATCAAAGATTAAGAAAGTGAAATCCAAAGGCCGCAAAAGCACCTCCGACGTTCCCTCAAGAAGAAGTGAGTTTCTAATTGATAAAATCAATGAAGAGGAAAGTGAATCATTGATTTCTTGCTTAGCAAGATTCTCGGAAGCCGAGAAGGTCCATTCTTCTAGTACTATGGATAGTACTAAAGTTAGGCCTATCTCAAATTATAGTGATAAAAAGGAGAATAAAGATTTAACTAGCTCGAATAGAAGGACGATAAGTCGTGTGAAGAATGTTGAGAGAGTGAGGTTTCAAAGTATTACGGAGAATCAAAGAGGAAAAGAAACGAAGGTTATGTCAAGTAGAATGGAAGAGAATCGAAGAGGAACCGAAACGAAGGTTATGTCAAGTAGAATGGAGGAGAAGGTAAAAGAAAGCTTTGCTTTGGTGAAAAAGTCAAAGGATCCTTACGAAGACTTCAAGAAATCAATGTTAGAGATGATAGAAGAGATGGAGATGTCCGAGGCCAAGGACTTGGAGCAACTATTGCAGTGTTTTTTGGCTCTAAATTCTAGAGATTATCATGGAGTAATTGTGCGAGCTTTCATGGAGATTTGGCAACAAATGTTCGTTTGGAATCCTAAGTCAGTAAAAAATCTTCAAACAAATGTTCAAACTAAGGATGTAGAAAATTAG

Protein sequence

>MS.gene064659.t1

MSKRFKMKFTIPSFQMCRSNNLSSFPGNPGPAIYRLSPVNSKAYDIGYPNLPASSPEHQCNVSSKIKKVKSKGRKSTSDVPSRRSEFLIDKINEEESESLISCLARFSEAEKVHSSSTMDSTKVRPISNYSDKKENKDLTSSNRRTISRVKNVERVRFQSITENQRGKETKVMSSRMEENRRGTETKVMSSRMEEKVKESFALVKKSKDPYEDFKKSMLEMIEEMEMSEAKDLEQLLQCFLALNSRDYHGVIVRAFMEIWQQMFVWNPKSVKNLQTNVQTKDVEN