Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064701.t1 | PNX61034.1 | 68.2 | 66 | 17 | 1 | 1 | 66 | 1 | 62 | 4.00E-16 | 94 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064701.t1 | A0A2K3MI54 | 68.2 | 66 | 17 | 1 | 1 | 66 | 4 | 65 | 2.9e-16 | 94.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049853 | MS.gene064701 | 0.809578 | 1.77E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064701.t1 | MTR_1g009820 | 50.000 | 86 | 33 | 2 | 1 | 86 | 1 | 76 | 1.12e-21 | 82.0 |
| MS.gene064701.t1 | MTR_8g089760 | 56.061 | 66 | 25 | 1 | 1 | 66 | 78 | 139 | 4.29e-19 | 77.0 |
| MS.gene064701.t1 | MTR_4g007830 | 56.061 | 66 | 25 | 1 | 1 | 66 | 261 | 322 | 2.78e-17 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGTTTCAAATCAACTTA+TGG | 0.227227 | 3.4:+97296971 | MS.gene064701:CDS |
| TAGGAATGATTGTGCGATTT+GGG | 0.304560 | 3.4:+97297163 | MS.gene064701:intron |
| TTTCATGGAAAACTTGTTGA+TGG | 0.314872 | 3.4:+97296925 | None:intergenic |
| ATCAAATGGATGATGGAAAA+AGG | 0.314997 | 3.4:+97297188 | MS.gene064701:CDS |
| TTAGGAATGATTGTGCGATT+TGG | 0.345924 | 3.4:+97297162 | MS.gene064701:intron |
| GTGCGATTTGGGTAATCAAA+TGG | 0.465432 | 3.4:+97297174 | MS.gene064701:CDS |
| ATGGATCCCTCTTTCTATGT+GGG | 0.495254 | 3.4:+97296944 | MS.gene064701:CDS |
| TTTGTTGCTCCACGTGGTTA+CGG | 0.500598 | 3.4:+97297016 | MS.gene064701:CDS |
| GATGGATCCCTCTTTCTATG+TGG | 0.506410 | 3.4:+97296943 | MS.gene064701:CDS |
| TTGGGTAATCAAATGGATGA+TGG | 0.515751 | 3.4:+97297181 | MS.gene064701:CDS |
| CACACCTGTTAGTGTAATTG+TGG | 0.546180 | 3.4:-97297305 | None:intergenic |
| GATCCCTCTTTCTATGTGGG+TGG | 0.554391 | 3.4:+97296947 | MS.gene064701:CDS |
| TGAACCACCCACATAGAAAG+AGG | 0.556566 | 3.4:-97296951 | None:intergenic |
| CCACGTGGTTACGGAAGTCA+AGG | 0.569221 | 3.4:+97297025 | MS.gene064701:CDS |
| TTTAAGTTTGTTGCTCCACG+TGG | 0.650344 | 3.4:+97297010 | MS.gene064701:CDS |
| GAACCACCCACATAGAAAGA+GGG | 0.662397 | 3.4:-97296950 | None:intergenic |
| CCTTGACTTCCGTAACCACG+TGG | 0.696038 | 3.4:-97297025 | None:intergenic |
| CACGTGGTTACGGAAGTCAA+GGG | 0.703622 | 3.4:+97297026 | MS.gene064701:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATACTCATAATTTTTTATTT+AGG | + | chr3.4:97297144-97297163 | MS.gene064701:intron | 10.0% |
| !!! | TTTTTTATATCTTACGAGTT+TGG | - | chr3.4:97297052-97297071 | None:intergenic | 20.0% |
| ! | TCAAGTTTCAAATCAACTTA+TGG | + | chr3.4:97296971-97296990 | MS.gene064701:CDS | 25.0% |
| !! | ATCAAATGGATGATGGAAAA+AGG | + | chr3.4:97297188-97297207 | MS.gene064701:CDS | 30.0% |
| TAGGAATGATTGTGCGATTT+GGG | + | chr3.4:97297163-97297182 | MS.gene064701:intron | 35.0% | |
| TTAGGAATGATTGTGCGATT+TGG | + | chr3.4:97297162-97297181 | MS.gene064701:intron | 35.0% | |
| TTGGGTAATCAAATGGATGA+TGG | + | chr3.4:97297181-97297200 | MS.gene064701:CDS | 35.0% | |
| ATGGATCCCTCTTTCTATGT+GGG | + | chr3.4:97296944-97296963 | MS.gene064701:CDS | 40.0% | |
| ! | GTGCGATTTGGGTAATCAAA+TGG | + | chr3.4:97297174-97297193 | MS.gene064701:CDS | 40.0% |
| !! | TTTAAGTTTGTTGCTCCACG+TGG | + | chr3.4:97297010-97297029 | MS.gene064701:CDS | 40.0% |
| GAACCACCCACATAGAAAGA+GGG | - | chr3.4:97296953-97296972 | None:intergenic | 45.0% | |
| GATGGATCCCTCTTTCTATG+TGG | + | chr3.4:97296943-97296962 | MS.gene064701:CDS | 45.0% | |
| TGAACCACCCACATAGAAAG+AGG | - | chr3.4:97296954-97296973 | None:intergenic | 45.0% | |
| TTTGTTGCTCCACGTGGTTA+CGG | + | chr3.4:97297016-97297035 | MS.gene064701:CDS | 45.0% | |
| CACGTGGTTACGGAAGTCAA+GGG | + | chr3.4:97297026-97297045 | MS.gene064701:CDS | 50.0% | |
| GATCCCTCTTTCTATGTGGG+TGG | + | chr3.4:97296947-97296966 | MS.gene064701:CDS | 50.0% | |
| CCACGTGGTTACGGAAGTCA+AGG | + | chr3.4:97297025-97297044 | MS.gene064701:CDS | 55.0% | |
| CCTTGACTTCCGTAACCACG+TGG | - | chr3.4:97297028-97297047 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 97296929 | 97297319 | 97296929 | ID=MS.gene064701 |
| chr3.4 | mRNA | 97296929 | 97297319 | 97296929 | ID=MS.gene064701.t1;Parent=MS.gene064701 |
| chr3.4 | exon | 97296929 | 97297056 | 97296929 | ID=MS.gene064701.t1.exon1;Parent=MS.gene064701.t1 |
| chr3.4 | CDS | 97296929 | 97297056 | 97296929 | ID=cds.MS.gene064701.t1;Parent=MS.gene064701.t1 |
| chr3.4 | exon | 97297166 | 97297319 | 97297166 | ID=MS.gene064701.t1.exon2;Parent=MS.gene064701.t1 |
| chr3.4 | CDS | 97297166 | 97297319 | 97297166 | ID=cds.MS.gene064701.t1;Parent=MS.gene064701.t1 |
| Gene Sequence |
| Protein sequence |
