AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049853


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049853.t1 MTR_6g465600 72.581 124 19 2 1 109 1 124 8.16e-57 178
MS.gene049853.t1 MTR_1g052720 47.581 124 47 3 1 109 1 121 1.16e-30 110
MS.gene049853.t1 MTR_7g050355 50.000 112 41 2 1 97 1 112 1.72e-29 110
MS.gene049853.t1 MTR_0246s0040 62.319 69 26 0 41 109 82 150 7.21e-27 100
MS.gene049853.t1 MTR_4g082235 55.294 85 25 2 1 72 1 85 3.72e-22 87.0
MS.gene049853.t1 MTR_0091s0080 51.515 66 32 0 42 107 256 321 3.58e-18 78.6
MS.gene049853.t1 MTR_4g117740 47.619 63 33 0 45 107 65 127 1.84e-16 71.6
MS.gene049853.t1 MTR_5g075200 43.478 69 39 0 40 108 62 130 9.65e-15 67.0
MS.gene049853.t1 MTR_2g038740 44.444 63 35 0 45 107 54 116 7.08e-14 66.2
MS.gene049853.t1 MTR_5g066660 40.789 76 43 1 36 109 43 118 3.12e-13 63.9
MS.gene049853.t1 MTR_2g100360 43.077 65 37 0 45 109 54 118 1.15e-12 62.4
MS.gene049853.t1 MTR_4g062370 39.683 63 38 0 45 107 58 120 2.25e-12 62.0
MS.gene049853.t1 MTR_8g085330 40.678 59 35 0 50 108 85 143 4.21e-12 60.8
MS.gene049853.t1 MTR_1g105765 39.683 63 38 0 45 107 55 117 4.72e-12 60.8
MS.gene049853.t1 MTR_3g070250 39.062 64 39 0 45 108 59 122 8.73e-12 60.1
MS.gene049853.t1 MTR_1g105780 38.095 63 39 0 45 107 55 117 8.81e-12 60.1
MS.gene049853.t1 MTR_8g064930 55.814 43 19 0 42 84 8 50 1.58e-11 55.1
MS.gene049853.t1 MTR_4g032560 40.000 65 39 0 44 108 82 146 2.10e-11 58.9
MS.gene049853.t1 MTR_4g032580 40.000 65 39 0 44 108 82 146 2.28e-11 58.9
MS.gene049853.t1 MTR_1g084880 39.062 64 39 0 45 108 54 117 2.36e-11 58.9
MS.gene049853.t1 MTR_3g048970 39.062 64 39 0 45 108 54 117 3.53e-11 58.2
MS.gene049853.t1 MTR_7g110090 40.000 65 39 0 44 108 53 117 6.15e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTAAGCAATGGAAAATTT+TGG 0.183630 4.4:-43944628 None:intergenic
GCTTGCTTTGCAAGAGGTTT+TGG 0.191388 4.4:-43943200 MS.gene049853:CDS
CAGAGGTGTTAGGAGGGATT+TGG 0.326630 4.4:-43943608 MS.gene049853:intron
TGGCATATTAACTGGTGGTT+GGG 0.398772 4.4:-43943054 MS.gene049853:CDS
GTGGGATTGACAGAGGTGTT+AGG 0.418369 4.4:-43943618 MS.gene049853:CDS
ATGGCATATTAACTGGTGGT+TGG 0.434678 4.4:-43943055 MS.gene049853:CDS
CTGCCTGCTTGCTTTGCAAG+AGG 0.447643 4.4:-43943206 MS.gene049853:CDS
TGATCACGCAGCATGACATA+TGG 0.478460 4.4:+43943164 None:intergenic
ACCAACAGCCTCACGATCCT+TGG 0.494590 4.4:+43944588 None:intergenic
GGATTGACAGAGGTGTTAGG+AGG 0.526695 4.4:-43943615 MS.gene049853:CDS
AAACCTCTTGCAAAGCAAGC+AGG 0.559763 4.4:+43943203 None:intergenic
GATTGACAGAGGTGTTAGGA+GGG 0.565582 4.4:-43943614 MS.gene049853:CDS
TCATAACCACAATGAGATTG+AGG 0.578617 4.4:-43943144 MS.gene049853:CDS
ATTAACTGGTGGTTGGGTCA+CGG 0.583188 4.4:-43943048 MS.gene049853:CDS
GGTGCTATATGTTTGATGAA+AGG 0.589079 4.4:-43944568 MS.gene049853:intron
TCAACAGTTACAGGCACTGA+TGG 0.591514 4.4:-43943640 MS.gene049853:intron
CAGTTACAGGCACTGATGGT+GGG 0.592227 4.4:-43943636 MS.gene049853:CDS
ACAGTTACAGGCACTGATGG+TGG 0.593464 4.4:-43943637 MS.gene049853:intron
TTGATGTTTGTGCAGCGTAA+GGG 0.593593 4.4:-43943233 MS.gene049853:intron
GCCAAGGATCGTGAGGCTGT+TGG 0.611772 4.4:-43944589 MS.gene049853:CDS
GAACTTCACAATGTATGCCA+AGG 0.644450 4.4:-43944605 MS.gene049853:CDS
AATGTATGCCAAGGATCGTG+AGG 0.653339 4.4:-43944596 MS.gene049853:CDS
TGGCAACCTCAATCTCATTG+TGG 0.670176 4.4:+43943138 None:intergenic
TGTTTGTGCAGCGTAAGGGA+AGG 0.701195 4.4:-43943229 MS.gene049853:intron
ACTGATGGTGGGATTGACAG+AGG 0.753991 4.4:-43943625 MS.gene049853:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTTATTCATCTAATATAT+TGG - chr4.4:43943675-43943694 MS.gene049853:intron 15.0%
!! ATGAAGAATAATAAAAGAAT+AGG - chr4.4:43944228-43944247 MS.gene049853:intron 15.0%
!!! TCATTTTTGAAAAACTTTTA+TGG - chr4.4:43944576-43944595 MS.gene049853:CDS 15.0%
!! AAGGTATATGTTATTATGTT+AGG + chr4.4:43943919-43943938 None:intergenic 20.0%
!! AATAATAAAAGAATAGGTCT+GGG - chr4.4:43944234-43944253 MS.gene049853:intron 20.0%
!! AATCTTAGTTTCTTATCTAT+TGG - chr4.4:43944285-43944304 MS.gene049853:intron 20.0%
!! ATTCATCTAATATATTGGAA+AGG - chr4.4:43943680-43943699 MS.gene049853:intron 20.0%
!! ATTGTTGAATATTCTATGTA+GGG + chr4.4:43943338-43943357 None:intergenic 20.0%
!! TAATACGTAATTATGATGAA+AGG - chr4.4:43943377-43943396 MS.gene049853:intron 20.0%
!! TTCAACAAATAAAGTTCTTA+AGG + chr4.4:43943112-43943131 None:intergenic 20.0%
!!! GTTTTATTACAGAGTTATTT+TGG + chr4.4:43943805-43943824 None:intergenic 20.0%
! ACACTAACAGCAATAAATAA+AGG + chr4.4:43943963-43943982 None:intergenic 25.0%
! ATAGCTTCACAATTACAAAT+AGG + chr4.4:43943177-43943196 None:intergenic 25.0%
! CATGATTATTCTTTAAACCA+TGG - chr4.4:43944372-43944391 MS.gene049853:intron 25.0%
! CATTGTTGAATATTCTATGT+AGG + chr4.4:43943339-43943358 None:intergenic 25.0%
! GAATAATAAAAGAATAGGTC+TGG - chr4.4:43944233-43944252 MS.gene049853:intron 25.0%
! GCTTGAAAATGAAATTACAT+AGG - chr4.4:43943727-43943746 MS.gene049853:intron 25.0%
! TTGATGTCTTTATCATAACT+GGG - chr4.4:43943431-43943450 MS.gene049853:intron 25.0%
! TTTGATGTCTTTATCATAAC+TGG - chr4.4:43943430-43943449 MS.gene049853:intron 25.0%
!! AACTTTTATGGCATATTAAC+TGG - chr4.4:43944588-43944607 MS.gene049853:CDS 25.0%
!! GTAATTATGATGAAAGGTAT+TGG - chr4.4:43943383-43943402 MS.gene049853:intron 25.0%
!! TGCTATTTACATTTGAACTA+TGG - chr4.4:43943463-43943482 MS.gene049853:intron 25.0%
AACAATGATATCATAACCCT+CGG - chr4.4:43943352-43943371 MS.gene049853:intron 30.0%
ATAATTACGTATTAAGCCGA+GGG + chr4.4:43943371-43943390 None:intergenic 30.0%
ATACAAACACACAACACTTA+AGG + chr4.4:43943938-43943957 None:intergenic 30.0%
ATTATTCTTTAAACCATGGC+AGG - chr4.4:43944376-43944395 MS.gene049853:intron 30.0%
CATGTAAAGACTTTAACATC+TGG - chr4.4:43944330-43944349 MS.gene049853:intron 30.0%
GAAAATGAAATTACATAGGC+TGG - chr4.4:43943731-43943750 MS.gene049853:intron 30.0%
GTTGCCATTGAAAAAAAGAA+CGG - chr4.4:43944528-43944547 MS.gene049853:intron 30.0%
TAAAGACTTTAACATCTGGT+TGG - chr4.4:43944334-43944353 MS.gene049853:intron 30.0%
TGATGTCTTTATCATAACTG+GGG - chr4.4:43943432-43943451 MS.gene049853:intron 30.0%
TTATGAGCACATTAAAATCC+CGG + chr4.4:43943624-43943643 None:intergenic 30.0%
TTGCCATTGAAAAAAAGAAC+GGG - chr4.4:43944529-43944548 MS.gene049853:intron 30.0%
! TTTTATGGCATATTAACTGG+TGG - chr4.4:43944591-43944610 MS.gene049853:CDS 30.0%
!! ATGTTTTCTATAGTTCTACC+GGG - chr4.4:43943603-43943622 MS.gene049853:intron 30.0%
!! GATGTTTTCTATAGTTCTAC+CGG - chr4.4:43943602-43943621 MS.gene049853:intron 30.0%
!!! TTTCCCGTTCTTTTTTTCAA+TGG + chr4.4:43944535-43944554 None:intergenic 30.0%
CATAATTACGTATTAAGCCG+AGG + chr4.4:43943372-43943391 None:intergenic 35.0%
CATGAATTAATTTCCTGCCA+TGG + chr4.4:43944392-43944411 None:intergenic 35.0%
CTATACAGTTAGAAGTCAAC+TGG + chr4.4:43943882-43943901 None:intergenic 35.0%
GTAGCAAGTAAATCAACTCA+CGG + chr4.4:43944107-43944126 None:intergenic 35.0%
GTGTTTCTTTATCCCATCAA+AGG - chr4.4:43943245-43943264 MS.gene049853:intron 35.0%
TCACAATTACAAATAGGTGC+AGG + chr4.4:43943171-43943190 None:intergenic 35.0%
TCATAACCACAATGAGATTG+AGG - chr4.4:43944506-43944525 MS.gene049853:intron 35.0%
TGTTTGCTTGACATCATGTT+TGG - chr4.4:43944187-43944206 MS.gene049853:intron 35.0%
! AAGTCTATTTTGCTGATGGA+TGG - chr4.4:43944553-43944572 MS.gene049853:intron 35.0%
! GGTGCTATATGTTTGATGAA+AGG - chr4.4:43943082-43943101 MS.gene049853:CDS 35.0%
! TTTTTCCTGTCAACAGTTAC+AGG - chr4.4:43944001-43944020 MS.gene049853:intron 35.0%
!! AGCATAAGTCTTATTCCATG+TGG + chr4.4:43944084-43944103 None:intergenic 35.0%
!! ATGCTGCTTTGTTTAGTGTT+TGG - chr4.4:43943501-43943520 MS.gene049853:intron 35.0%
!! GGTTTGTGTTTGTTGTTCTT+TGG - chr4.4:43943296-43943315 MS.gene049853:intron 35.0%
ATGGCATATTAACTGGTGGT+TGG - chr4.4:43944595-43944614 MS.gene049853:CDS 40.0%
GAACTTCACAATGTATGCCA+AGG - chr4.4:43943045-43943064 MS.gene049853:CDS 40.0%
GTGAATGCAGAATCTCAAAC+AGG - chr4.4:43943269-43943288 MS.gene049853:intron 40.0%
TCTGCATTCACACCTTTGAT+GGG + chr4.4:43943260-43943279 None:intergenic 40.0%
TGGCATATTAACTGGTGGTT+GGG - chr4.4:43944596-43944615 MS.gene049853:CDS 40.0%
TTCTGCATTCACACCTTTGA+TGG + chr4.4:43943261-43943280 None:intergenic 40.0%
TTGATGTTTGTGCAGCGTAA+GGG - chr4.4:43944417-43944436 MS.gene049853:intron 40.0%
TTTGATGTTTGTGCAGCGTA+AGG - chr4.4:43944416-43944435 MS.gene049853:intron 40.0%
! AATAGGTCTGGGATACTACA+TGG - chr4.4:43944245-43944264 MS.gene049853:intron 40.0%
! GGGAAAGTCTATTTTGCTGA+TGG - chr4.4:43944549-43944568 MS.gene049853:intron 40.0%
!!! AGAGGTTTTGGTTTGCAAGT+TGG - chr4.4:43944462-43944481 MS.gene049853:intron 40.0%
AAACCTCTTGCAAAGCAAGC+AGG + chr4.4:43944450-43944469 None:intergenic 45.0%
AATGTATGCCAAGGATCGTG+AGG - chr4.4:43943054-43943073 MS.gene049853:CDS 45.0%
ACATCTGGTTGGAGTAGTCA+TGG - chr4.4:43944345-43944364 MS.gene049853:intron 45.0%
ATTAACTGGTGGTTGGGTCA+CGG - chr4.4:43944602-43944621 MS.gene049853:CDS 45.0%
CATCTGGTTGGAGTAGTCAT+GGG - chr4.4:43944346-43944365 MS.gene049853:intron 45.0%
GCAGAATCTCAAACAGGAAG+TGG - chr4.4:43943275-43943294 MS.gene049853:intron 45.0%
GGTGCAGGTTAAGAAAACTC+AGG + chr4.4:43943156-43943175 None:intergenic 45.0%
TGATCACGCAGCATGACATA+TGG + chr4.4:43944489-43944508 None:intergenic 45.0%
TGGCAACCTCAATCTCATTG+TGG + chr4.4:43944515-43944534 None:intergenic 45.0%
! GCTTGCTTTGCAAGAGGTTT+TGG - chr4.4:43944450-43944469 MS.gene049853:intron 45.0%
!! ATTCCATGTGGAAAGTGCTG+AGG + chr4.4:43944072-43944091 None:intergenic 45.0%
!! GATTGACAGAGGTGTTAGGA+GGG - chr4.4:43944036-43944055 MS.gene049853:intron 45.0%
!! TCAACAGTTACAGGCACTGA+TGG - chr4.4:43944010-43944029 MS.gene049853:intron 45.0%
CAGTGCCTGTAACTGTTGAC+AGG + chr4.4:43944009-43944028 None:intergenic 50.0%
TAGCCTCAGCACTTTCCACA+TGG - chr4.4:43944066-43944085 MS.gene049853:intron 50.0%
TGTTTGTGCAGCGTAAGGGA+AGG - chr4.4:43944421-43944440 MS.gene049853:intron 50.0%
!! ACAGTTACAGGCACTGATGG+TGG - chr4.4:43944013-43944032 MS.gene049853:intron 50.0%
!! ACTGATGGTGGGATTGACAG+AGG - chr4.4:43944025-43944044 MS.gene049853:intron 50.0%
!! CAGAGGTGTTAGGAGGGATT+TGG - chr4.4:43944042-43944061 MS.gene049853:intron 50.0%
!! CAGTTACAGGCACTGATGGT+GGG - chr4.4:43944014-43944033 MS.gene049853:intron 50.0%
!! GGATTGACAGAGGTGTTAGG+AGG - chr4.4:43944035-43944054 MS.gene049853:intron 50.0%
!! GTGGGATTGACAGAGGTGTT+AGG - chr4.4:43944032-43944051 MS.gene049853:intron 50.0%
ACCAACAGCCTCACGATCCT+TGG + chr4.4:43943065-43943084 None:intergenic 55.0%
CTGCCTGCTTGCTTTGCAAG+AGG - chr4.4:43944444-43944463 MS.gene049853:intron 55.0%
GCCAAGGATCGTGAGGCTGT+TGG - chr4.4:43943061-43943080 MS.gene049853:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 43943031 43944641 43943031 ID=MS.gene049853
chr4.4 mRNA 43943031 43944641 43943031 ID=MS.gene049853.t1;Parent=MS.gene049853
chr4.4 exon 43944569 43944641 43944569 ID=MS.gene049853.t1.exon1;Parent=MS.gene049853.t1
chr4.4 CDS 43944569 43944641 43944569 ID=cds.MS.gene049853.t1;Parent=MS.gene049853.t1
chr4.4 exon 43943609 43943655 43943609 ID=MS.gene049853.t1.exon2;Parent=MS.gene049853.t1
chr4.4 CDS 43943609 43943655 43943609 ID=cds.MS.gene049853.t1;Parent=MS.gene049853.t1
chr4.4 exon 43943031 43943240 43943031 ID=MS.gene049853.t1.exon3;Parent=MS.gene049853.t1
chr4.4 CDS 43943031 43943240 43943031 ID=cds.MS.gene049853.t1;Parent=MS.gene049853.t1
Gene Sequence

>MS.gene049853

ATGGAAAATTTTGGGAACTTCACAATGTATGCCAAGGATCGTGAGGCTGTTGGTGCTATATGTTTGATGAAAGGTGCACCTTAAGAACTTTATTTGTTGAATGCTTGATGTTTGATTCATAACCTGAGTTTTCTTAACCTGCACCTATTTGTAATTGTGAAGCTATTTTATCTTTTTGATAGTGTGTAAAGTTGTTGTTCAATTTCTTATATATGTGTTTCTTTATCCCATCAAAGGTGTGAATGCAGAATCTCAAACAGGAAGTGGTTTGTGTTTGTTGTTCTTTGGATTGCTGTAGTTTTTACCCTACATAGAATATTCAACAATGATATCATAACCCTCGGCTTAATACGTAATTATGATGAAAGGTATTGGTGAATTAAATATAGATGATGAATGTTTGATGTCTTTATCATAACTGGGGTTTGTTTTTGCTATTTACATTTGAACTATGGAATCAGAAATCATTTATGCTGCTTTGTTTAGTGTTTGGTTAATTTTTTGTGTCTAATTTTTATCACTAAGTGTGAATACTTATGATGTTTACTTTCAGTGATTTACGTGCTCATTAGATGTTTTCTATAGTTCTACCGGGATTTTAATGTGCTCATAACACTAATATGCAAATGTTTGCAACATTATCAAGTTTATTCATCTAATATATTGGAAAGGCAAAAGCATTCTCATTGATCAATTGCTTGAAAATGAAATTACATAGGCTGGTGTTTATATATAGATAAACACAGTTGACAAAAAACTAGTTTAAATCTGCCAAAATAACTCTGTAATAAAACTATTAATGACAGAATTAACTCTAATAAAAAAACTTATTAACTTGCTAGTAAAAACCAGTTGACTTCTAACTGTATAGCTGTGAGTTAATCTCCTAACATAATAACATATACCTTAAGTGTTGTGTGTTTGTATTTCCTTTATTTATTGCTGTTAGTGTGTTTTCTTATATGTGCAATTTTTCCTGTCAACAGTTACAGGCACTGATGGTGGGATTGACAGAGGTGTTAGGAGGGATTTGGTTAGCCTCAGCACTTTCCACATGGAATAAGACTTATGCTCCGTGAGTTGATTTACTTGCTACTAATTAGTAATTTCTTTAAGTATGTGCTATGTGCTACTTTGATTTCGTTCATTTGTTGTGTGTTTGCTTGACATCATGTTTGGTTTAAACATAATATGTGTATGAAGAATAATAAAAGAATAGGTCTGGGATACTACATGGTAAACATGTCATGATTTAATCTTAGTTTCTTATCTATTGGTGTTATTAAGATGCTCATCATACATGTAAAGACTTTAACATCTGGTTGGAGTAGTCATGGGTTGCATGATTATTCTTTAAACCATGGCAGGAAATTAATTCATGATCATTTGATGTTTGTGCAGCGTAAGGGAAGGCTGCCTGCTTGCTTTGCAAGAGGTTTTGGTTTGCAAGTTGGTCCATATGTCATGCTGCGTGATCATAACCACAATGAGATTGAGGTTGCCATTGAAAAAAAGAACGGGAAAGTCTATTTTGCTGATGGATGGTCATTTTTGAAAAACTTTTATGGCATATTAACTGGTGGTTGGGTCACGGTCATCTTTGCAAATTGA

Protein sequence

>MS.gene049853.t1

MENFGNFTMYAKDREAVGAICLMKVTGTDGGIDRGVRRDLRKGRLPACFARGFGLQVGPYVMLRDHNHNEIEVAIEKKNGKVYFADGWSFLKNFYGILTGGWVTVIFAN