Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065191.t1 | XP_013464717.1 | 97.1 | 580 | 14 | 1 | 1 | 577 | 54 | 633 | 1.8e-309 | 1071.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065191.t1 | Q94AN2 | 68.8 | 583 | 176 | 3 | 1 | 577 | 63 | 645 | 4.3e-240 | 832.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065191.t1 | A0A072VAC4 | 97.1 | 580 | 14 | 1 | 1 | 577 | 54 | 633 | 1.3e-309 | 1071.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057669 | MS.gene065191 | 0.817277 | 3.59E-52 | -1.69E-46 |
| MS.gene059843 | MS.gene065191 | 0.981495 | 9.96E-153 | -1.69E-46 |
| MS.gene059844 | MS.gene065191 | 0.88465 | 1.60E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 142 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAGGAGTTTCTCATTTC+TGG | 0.112317 | 2.4:+18107971 | None:intergenic |
| GTTCTACATGTTATGGATTT+TGG | 0.116753 | 2.4:-18105148 | MS.gene065191:CDS |
| TTCACCTGTTTGGTTGTTAT+TGG | 0.137699 | 2.4:-18104972 | MS.gene065191:CDS |
| GTCATATTTCCTAGTGTTAA+TGG | 0.160201 | 2.4:-18107923 | MS.gene065191:intron |
| TTCTCACTCAGCTGTCTTAA+AGG | 0.173733 | 2.4:-18107499 | MS.gene065191:intron |
| ATCTGTCGCCTCTTATTATT+GGG | 0.194594 | 2.4:-18104905 | MS.gene065191:CDS |
| TTGAATTGGCTTCATTCTTT+TGG | 0.195265 | 2.4:+18100008 | None:intergenic |
| GATCTGTCGCCTCTTATTAT+TGG | 0.203692 | 2.4:-18104906 | MS.gene065191:CDS |
| CGCGTGAAGATTCACTTGTC+TGG | 0.248425 | 2.4:-18108078 | MS.gene065191:CDS |
| ATCGAACGAAAAGGAATTTC+AGG | 0.255259 | 2.4:+18104630 | None:intergenic |
| GCCATCCTCTTTCACCTGTT+TGG | 0.266801 | 2.4:-18104982 | MS.gene065191:CDS |
| TCACCTGTTTGGTTGTTATT+GGG | 0.270892 | 2.4:-18104971 | MS.gene065191:CDS |
| CCCGGCCGGAGCTGAATAAA+TGG | 0.271388 | 2.4:+18105118 | None:intergenic |
| ACAATGTTCTACTACCTAAA+AGG | 0.276175 | 2.4:-18106999 | MS.gene065191:intron |
| TTGCAGTGTTCTACATGTTA+TGG | 0.282895 | 2.4:-18105155 | MS.gene065191:CDS |
| CTTTGTTTCTCTCTGTGATT+TGG | 0.295140 | 2.4:-18107100 | MS.gene065191:CDS |
| TGGTTTATTAGGCTTACTTT+TGG | 0.316715 | 2.4:-18108265 | None:intergenic |
| GATCCTTACATTCACATATA+TGG | 0.329782 | 2.4:-18106142 | MS.gene065191:CDS |
| ATCAGCTATTGCAACAGATT+TGG | 0.332889 | 2.4:-18104106 | MS.gene065191:CDS |
| CTTTGTTAATATAGAATCTT+GGG | 0.341746 | 2.4:-18100049 | MS.gene065191:intron |
| ACTTTGTTAATATAGAATCT+TGG | 0.352673 | 2.4:-18100050 | MS.gene065191:intron |
| TTTCTGCAGATTGCTATAAC+TGG | 0.353625 | 2.4:-18104147 | MS.gene065191:intron |
| TATTGTTCGGCGCATCCTTA+TGG | 0.353828 | 2.4:-18105932 | MS.gene065191:CDS |
| ATTCCGGTTCACTGATTTGA+TGG | 0.357743 | 2.4:+18104404 | None:intergenic |
| AGTGAATCTTCACGCGGAAC+AGG | 0.360197 | 2.4:+18108085 | None:intergenic |
| CTTCATGTCTTTCAGTTGGA+TGG | 0.371868 | 2.4:-18106204 | MS.gene065191:intron |
| CAAATGTGTCTTTAAGCCTT+TGG | 0.373843 | 2.4:-18107590 | MS.gene065191:CDS |
| TTGAAGCTCTAGCAATGAAT+TGG | 0.374024 | 2.4:+18107610 | None:intergenic |
| TATGGCAACTTCTGTCCTTA+AGG | 0.379154 | 2.4:-18105914 | MS.gene065191:intron |
| AGGATACCATATGGTATGAT+TGG | 0.381943 | 2.4:+18105177 | None:intergenic |
| ATCAATTGGAGCTCAAATAC+TGG | 0.383717 | 2.4:-18108195 | MS.gene065191:CDS |
| AGTATTGGTTCTCTAGCTCT+TGG | 0.387761 | 2.4:-18104576 | MS.gene065191:CDS |
| CCAGTCATGTGCCTGACAAC+TGG | 0.392677 | 2.4:-18104482 | MS.gene065191:CDS |
| GCATGCAGGCCTTCATCAAT+TGG | 0.395402 | 2.4:-18108209 | MS.gene065191:CDS |
| GTAACACATAAGTCTCTTCA+TGG | 0.400123 | 2.4:+18104599 | None:intergenic |
| AACTCCTCTCTTCAACTTCA+AGG | 0.407170 | 2.4:-18107959 | MS.gene065191:CDS |
| GTCCGTTACACACCGCATAT+TGG | 0.408374 | 2.4:-18105009 | MS.gene065191:CDS |
| TTTAAGCCTTTGGCAGCAGA+TGG | 0.410816 | 2.4:-18107580 | MS.gene065191:CDS |
| TTACCATGATATAAGCATTC+CGG | 0.413773 | 2.4:+18104388 | None:intergenic |
| TCCATTTATTCAGCTCCGGC+CGG | 0.414225 | 2.4:-18105119 | MS.gene065191:CDS |
| GAAACAACAATATCTCCTTC+AGG | 0.416166 | 2.4:+18108043 | None:intergenic |
| TTACATCAATTGCTATTGTT+CGG | 0.423458 | 2.4:-18105945 | MS.gene065191:CDS |
| TTGTTGTTTCAATGGATGAT+TGG | 0.424634 | 2.4:-18108030 | MS.gene065191:CDS |
| GGCTCATCAACAGTGATTGC+TGG | 0.426887 | 2.4:-18104928 | MS.gene065191:CDS |
| TGCTCGCAGTATATGCTTGT+TGG | 0.427523 | 2.4:-18108113 | MS.gene065191:CDS |
| CTTCCATATATGTGAATGTA+AGG | 0.429797 | 2.4:+18106139 | None:intergenic |
| TATAGAAAACATACCACAAC+AGG | 0.431860 | 2.4:+18103919 | None:intergenic |
| TAATATAGAATCTTGGGATA+AGG | 0.433751 | 2.4:-18100043 | MS.gene065191:intron |
| AGACTTATGTGTTACAGTAT+TGG | 0.440425 | 2.4:-18104591 | MS.gene065191:CDS |
| AGGAGATATTGTTGTTTCAA+TGG | 0.446951 | 2.4:-18108038 | MS.gene065191:CDS |
| ATTTCGTTGCTGCAATGCCT+TGG | 0.449324 | 2.4:-18107061 | MS.gene065191:CDS |
| GCCTTCCCGATCCAGTTGTC+AGG | 0.450311 | 2.4:+18104471 | None:intergenic |
| CCATTTATTCAGCTCCGGCC+GGG | 0.453264 | 2.4:-18105118 | MS.gene065191:CDS |
| GCGTGAAGATTCACTTGTCT+GGG | 0.458171 | 2.4:-18108077 | MS.gene065191:CDS |
| GGAGATGTTATATTGTTCCT+TGG | 0.458625 | 2.4:-18104042 | MS.gene065191:CDS |
| TGTCTTTCAGTTGGATGGAT+AGG | 0.459269 | 2.4:-18106199 | MS.gene065191:intron |
| ATGTGCCTGACAACTGGATC+GGG | 0.459928 | 2.4:-18104476 | MS.gene065191:CDS |
| CCAGTTGTCAGGCACATGAC+TGG | 0.460652 | 2.4:+18104482 | None:intergenic |
| GACTGGAGACTTTCAGTTTG+CGG | 0.460819 | 2.4:+18104499 | None:intergenic |
| AAGAATAACATCCTACGGAT+TGG | 0.462361 | 2.4:-18104081 | MS.gene065191:CDS |
| ATCTCCACTCTTGCCTGTTG+TGG | 0.469830 | 2.4:-18103932 | MS.gene065191:intron |
| GTTACACAATTTCTCATAGC+AGG | 0.473826 | 2.4:-18104949 | MS.gene065191:CDS |
| TGGCCTGTGTTGATCGTTTG+TGG | 0.475648 | 2.4:-18107134 | MS.gene065191:CDS |
| AATAGCAATTGATGTAAGAA+TGG | 0.476234 | 2.4:+18105951 | None:intergenic |
| AAGGCATTGCAGCAACGAAA+TGG | 0.477500 | 2.4:+18107063 | None:intergenic |
| CAACCATGATAAAAGTCATA+AGG | 0.481385 | 2.4:+18105977 | None:intergenic |
| AGAATAACATCCTACGGATT+GGG | 0.481832 | 2.4:-18104080 | MS.gene065191:CDS |
| TTTCTCCATTTATTCAGCTC+CGG | 0.482821 | 2.4:-18105123 | MS.gene065191:CDS |
| TCTAATCAAGTTTATCAAAG+TGG | 0.482823 | 2.4:-18108166 | MS.gene065191:CDS |
| ATCATGACCCCAATAATAAG+AGG | 0.483599 | 2.4:+18104897 | None:intergenic |
| GGATACCATATGGTATGATT+GGG | 0.485559 | 2.4:+18105178 | None:intergenic |
| ATTGGGCATGTCAATGTGAT+TGG | 0.488268 | 2.4:-18104063 | MS.gene065191:CDS |
| CATATAACCTCATGGAAAAG+AGG | 0.493772 | 2.4:-18105062 | MS.gene065191:CDS |
| AGTTTATCAAAGTGGATTGA+AGG | 0.494393 | 2.4:-18108158 | MS.gene065191:CDS |
| TAACCCAATAACAACCAAAC+AGG | 0.495118 | 2.4:+18104968 | None:intergenic |
| GGACCATCAAATCAGTGAAC+CGG | 0.495664 | 2.4:-18104407 | MS.gene065191:CDS |
| AACAACCAAACAGGTGAAAG+AGG | 0.496513 | 2.4:+18104977 | None:intergenic |
| TGAATGGACTTATCAATGAC+AGG | 0.497226 | 2.4:+18107534 | None:intergenic |
| AGGATCATGCTCACCAATGA+TGG | 0.503621 | 2.4:+18106159 | None:intergenic |
| CTGTGTTGATCGTTTGTGGT+GGG | 0.508004 | 2.4:-18107130 | MS.gene065191:CDS |
| GATGCTATAACACCCATCAT+TGG | 0.509677 | 2.4:-18106172 | MS.gene065191:CDS |
| CTGCTGCTCATATAACCTCA+TGG | 0.512269 | 2.4:-18105070 | MS.gene065191:CDS |
| CTCCAATATGCGGTGTGTAA+CGG | 0.517980 | 2.4:+18105007 | None:intergenic |
| ATTTGAGCTCCAATTGATGA+AGG | 0.519919 | 2.4:+18108200 | None:intergenic |
| TGGTTAAGAATAACATCCTA+CGG | 0.524720 | 2.4:-18104086 | MS.gene065191:CDS |
| GAGGAAAAGATCGAACGAAA+AGG | 0.525477 | 2.4:+18104621 | None:intergenic |
| TAGGTTGCTGCAAAAGTGAT+AGG | 0.528922 | 2.4:-18105225 | MS.gene065191:CDS |
| GTGATCTGTGATCATTGTTG+TGG | 0.533821 | 2.4:-18105039 | MS.gene065191:CDS |
| CAGAGATACATGGCTGACAT+AGG | 0.535311 | 2.4:-18107164 | MS.gene065191:CDS |
| TGCTGCAATGCCTTGGATAA+CGG | 0.535787 | 2.4:-18107054 | MS.gene065191:CDS |
| GAACCGGAATGCTTATATCA+TGG | 0.540249 | 2.4:-18104391 | MS.gene065191:intron |
| AACACTGCAAGGATACCATA+TGG | 0.542211 | 2.4:+18105168 | None:intergenic |
| TAAGGACAGAAGTTGCCATA+AGG | 0.544009 | 2.4:+18105917 | None:intergenic |
| GTCATTCTGAACAACCCGGC+CGG | 0.546462 | 2.4:+18105104 | None:intergenic |
| TTAAGCCTTTGGCAGCAGAT+GGG | 0.546941 | 2.4:-18107579 | MS.gene065191:CDS |
| AGAATTGATATACTTGTGAA+TGG | 0.547515 | 2.4:+18107518 | None:intergenic |
| CATACCACAACAGGCAAGAG+TGG | 0.553297 | 2.4:+18103928 | None:intergenic |
| AGCCTTTGGCAGCAGATGGG+TGG | 0.556537 | 2.4:-18107576 | MS.gene065191:CDS |
| AATCTATTTACAGAGATACA+TGG | 0.560146 | 2.4:-18107174 | MS.gene065191:intron |
| TGATCTGTGATCATTGTTGT+GGG | 0.560312 | 2.4:-18105038 | MS.gene065191:CDS |
| AGTTTCTCATTTCTGGTGTG+AGG | 0.565020 | 2.4:+18107978 | None:intergenic |
| CTCCACCCATCTGCTGCCAA+AGG | 0.570395 | 2.4:+18107574 | None:intergenic |
| TATATGAGCAGCAGTTAGAG+TGG | 0.582084 | 2.4:+18105079 | None:intergenic |
| AATAAAACCACCGTTATCCA+AGG | 0.582647 | 2.4:+18107044 | None:intergenic |
| CTTACATTCACATATATGGA+AGG | 0.582819 | 2.4:-18106138 | MS.gene065191:CDS |
| GGATCATGCTCACCAATGAT+GGG | 0.585240 | 2.4:+18106160 | None:intergenic |
| ATATAACCTCATGGAAAAGA+GGG | 0.587592 | 2.4:-18105061 | MS.gene065191:CDS |
| CATGTGCCTGACAACTGGAT+CGG | 0.591232 | 2.4:-18104477 | MS.gene065191:CDS |
| GACTACAAAGGAAATGTGTG+TGG | 0.591493 | 2.4:-18108238 | MS.gene065191:CDS |
| ACCAAACAGGTGAAAGAGGA+TGG | 0.592919 | 2.4:+18104981 | None:intergenic |
| TTGGGGTCATGATGAAGCAT+CGG | 0.601645 | 2.4:-18104887 | MS.gene065191:intron |
| TGGGTTTGTGATTATCCTGA+AGG | 0.603089 | 2.4:-18108058 | MS.gene065191:CDS |
| CAAATCACAGAGAGAAACAA+AGG | 0.607234 | 2.4:+18107101 | None:intergenic |
| GTGAATCTTCACGCGGAACA+GGG | 0.608187 | 2.4:+18108086 | None:intergenic |
| CCTGTGTTGATCGTTTGTGG+TGG | 0.608362 | 2.4:-18107131 | MS.gene065191:CDS |
| GTGTGTGGGGACAAGCATGC+AGG | 0.612803 | 2.4:-18108223 | MS.gene065191:CDS |
| CATTGACATGCCCAATCCGT+AGG | 0.613058 | 2.4:+18104070 | None:intergenic |
| GGATAGTCAGTTGAAACTTG+CGG | 0.621722 | 2.4:-18108137 | MS.gene065191:CDS |
| ACTACAAAGGAAATGTGTGT+GGG | 0.628516 | 2.4:-18108237 | MS.gene065191:CDS |
| TTACATTCACATATATGGAA+GGG | 0.631269 | 2.4:-18106137 | MS.gene065191:intron |
| ACTCTATGCACTCGTTACAC+AGG | 0.632524 | 2.4:+18100480 | None:intergenic |
| ATTATCTCACCATTAACACT+AGG | 0.639078 | 2.4:+18107914 | None:intergenic |
| GGAAATATGACAGGATAACA+AGG | 0.643638 | 2.4:+18107935 | None:intergenic |
| TTAACACTAGGAAATATGAC+AGG | 0.644351 | 2.4:+18107926 | None:intergenic |
| ATACATGGCTGACATAGGAA+AGG | 0.648575 | 2.4:-18107159 | MS.gene065191:CDS |
| TGCAATGCCTTGGATAACGG+TGG | 0.651035 | 2.4:-18107051 | MS.gene065191:CDS |
| ATAACATGTAGAACACTGCA+AGG | 0.651424 | 2.4:+18105157 | None:intergenic |
| AGGTCCTTGAAGTTGAAGAG+AGG | 0.651499 | 2.4:+18107955 | None:intergenic |
| TCTGTCGCCTCTTATTATTG+GGG | 0.654026 | 2.4:-18104904 | MS.gene065191:CDS |
| GGCAGTCATTCTGAACAACC+CGG | 0.660821 | 2.4:+18105100 | None:intergenic |
| GCATTGCAGCAACGAAATGG+CGG | 0.662646 | 2.4:+18107066 | None:intergenic |
| GCCTGACAACTGGATCGGGA+AGG | 0.663993 | 2.4:-18104472 | MS.gene065191:CDS |
| CCACCACAAACGATCAACAC+AGG | 0.667874 | 2.4:+18107131 | None:intergenic |
| AACGAGTGCATAGAGTGGCA+AGG | 0.670778 | 2.4:-18100473 | MS.gene065191:CDS |
| GAATGGACTTATCAATGACA+GGG | 0.677790 | 2.4:+18107535 | None:intergenic |
| TGTGTAACGAGTGCATAGAG+TGG | 0.680598 | 2.4:-18100478 | MS.gene065191:CDS |
| TGGCTGACATAGGAAAGGCA+TGG | 0.696862 | 2.4:-18107154 | MS.gene065191:CDS |
| CTACAAAGGAAATGTGTGTG+GGG | 0.698776 | 2.4:-18108236 | MS.gene065191:CDS |
| AGGATGGCAACTCCAATATG+CGG | 0.707650 | 2.4:+18104997 | None:intergenic |
| ACACATAAGTCTCTTCATGG+AGG | 0.723284 | 2.4:+18104602 | None:intergenic |
| CAGACAAGTGAATCTTCACG+CGG | 0.725966 | 2.4:+18108079 | None:intergenic |
| ACTTGTGAGTGTCTAACATG+AGG | 0.749930 | 2.4:+18103980 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 18100019 | 18108276 | 18100019 | ID=MS.gene065191 |
| chr2.4 | mRNA | 18100019 | 18108276 | 18100019 | ID=MS.gene065191.t1;Parent=MS.gene065191 |
| chr2.4 | exon | 18107924 | 18108276 | 18107924 | ID=MS.gene065191.t1.exon1;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18107924 | 18108276 | 18107924 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| chr2.4 | exon | 18107500 | 18107645 | 18107500 | ID=MS.gene065191.t1.exon2;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18107500 | 18107645 | 18107500 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| chr2.4 | exon | 18107000 | 18107183 | 18107000 | ID=MS.gene065191.t1.exon3;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18107000 | 18107183 | 18107000 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| chr2.4 | exon | 18106138 | 18106211 | 18106138 | ID=MS.gene065191.t1.exon4;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18106138 | 18106211 | 18106138 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| chr2.4 | exon | 18105915 | 18106028 | 18105915 | ID=MS.gene065191.t1.exon5;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18105915 | 18106028 | 18105915 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| chr2.4 | exon | 18104888 | 18105244 | 18104888 | ID=MS.gene065191.t1.exon6;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18104888 | 18105244 | 18104888 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| chr2.4 | exon | 18104392 | 18104652 | 18104392 | ID=MS.gene065191.t1.exon7;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18104392 | 18104652 | 18104392 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| chr2.4 | exon | 18103933 | 18104160 | 18103933 | ID=MS.gene065191.t1.exon8;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18103933 | 18104160 | 18103933 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| chr2.4 | exon | 18100418 | 18100513 | 18100418 | ID=MS.gene065191.t1.exon9;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18100418 | 18100513 | 18100418 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| chr2.4 | exon | 18100019 | 18100057 | 18100019 | ID=MS.gene065191.t1.exon10;Parent=MS.gene065191.t1 |
| chr2.4 | CDS | 18100019 | 18100057 | 18100019 | ID=cds.MS.gene065191.t1;Parent=MS.gene065191.t1 |
| Gene Sequence |
| Protein sequence |
