Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065266.t1 | RHN75073.1 | 91.6 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 5.40E-34 | 153.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065266.t1 | A0A072VB89 | 91.6 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 3.9e-34 | 153.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059789 | MS.gene065266 | 0.882856 | 7.33E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065266.t1 | MTR_2g079000 | 91.566 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 4.55e-42 | 137 |
| MS.gene065266.t1 | MTR_5g012820 | 66.279 | 86 | 26 | 3 | 3 | 87 | 7 | 90 | 4.28e-24 | 93.6 |
| MS.gene065266.t1 | MTR_5g012820 | 67.949 | 78 | 23 | 2 | 11 | 87 | 4 | 80 | 6.44e-23 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065266.t1 | AT5G05210 | 57.627 | 59 | 24 | 1 | 29 | 87 | 24 | 81 | 1.91e-13 | 64.3 |
| MS.gene065266.t1 | AT5G05210 | 57.627 | 59 | 24 | 1 | 29 | 87 | 16 | 73 | 2.21e-13 | 63.9 |
Find 18 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACGAGAAGGCATGGTTTC+AGG | 0.217653 | 2.4:+19340697 | MS.gene065266:CDS |
| TTTATTACAATGAGAGATTC+CGG | 0.340961 | 2.4:+19340530 | None:intergenic |
| CAGAAGGGTGTTGCAGATAT+TGG | 0.354363 | 2.4:+19340575 | MS.gene065266:CDS |
| GTTGGTAGATAAAACTTAGC+TGG | 0.463279 | 2.4:-19340668 | None:intergenic |
| AACGAGAAGGCATGGTTTCA+GGG | 0.469679 | 2.4:+19340698 | MS.gene065266:CDS |
| TATTGGTGCTTGTCAAGACT+TGG | 0.497123 | 2.4:+19340592 | MS.gene065266:CDS |
| GTTAGGGTCTGAACTCTAAA+CGG | 0.521104 | 2.4:-19340790 | None:intergenic |
| GCCTTCTCGTTATCATCAGT+TGG | 0.521411 | 2.4:-19340686 | None:intergenic |
| AATGAGAGATTCCGGAGTGA+TGG | 0.528245 | 2.4:+19340538 | None:intergenic |
| GGAGATTAAGAAGAAGCAGA+AGG | 0.532482 | 2.4:+19340559 | MS.gene065266:CDS |
| GAGATTAAGAAGAAGCAGAA+GGG | 0.542455 | 2.4:+19340560 | MS.gene065266:CDS |
| AAGCAAGGGTGCAAAAGCTA+AGG | 0.564446 | 2.4:+19340724 | MS.gene065266:CDS |
| TAGACAATATTATGAAGTCT+CGG | 0.586642 | 2.4:+19340762 | MS.gene065266:CDS |
| ATGGTTTCAGGGTCTAAGCA+AGG | 0.591717 | 2.4:+19340709 | MS.gene065266:CDS |
| CTTCTTAATCTCCATCACTC+CGG | 0.599988 | 2.4:-19340549 | None:intergenic |
| CTGATGATAACGAGAAGGCA+TGG | 0.624704 | 2.4:+19340690 | MS.gene065266:CDS |
| TGGTTTCAGGGTCTAAGCAA+GGG | 0.633440 | 2.4:+19340710 | MS.gene065266:CDS |
| ACCAACTGATGATAACGAGA+AGG | 0.659759 | 2.4:+19340685 | MS.gene065266:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATCAATTTGTCGAAAAAAT+GGG | - | chr2.4:19340639-19340658 | None:intergenic | 25.0% |
| ! | TAGACAATATTATGAAGTCT+CGG | + | chr2.4:19340762-19340781 | MS.gene065266:CDS | 25.0% |
| CGATCAATTTGTCGAAAAAA+TGG | - | chr2.4:19340640-19340659 | None:intergenic | 30.0% | |
| GAGATTAAGAAGAAGCAGAA+GGG | + | chr2.4:19340560-19340579 | MS.gene065266:CDS | 35.0% | |
| ! | GTTGGTAGATAAAACTTAGC+TGG | - | chr2.4:19340671-19340690 | None:intergenic | 35.0% |
| CTTCTTAATCTCCATCACTC+CGG | - | chr2.4:19340552-19340571 | None:intergenic | 40.0% | |
| GGAGATTAAGAAGAAGCAGA+AGG | + | chr2.4:19340559-19340578 | MS.gene065266:CDS | 40.0% | |
| ! | ACCAACTGATGATAACGAGA+AGG | + | chr2.4:19340685-19340704 | MS.gene065266:CDS | 40.0% |
| ! | TATTGGTGCTTGTCAAGACT+TGG | + | chr2.4:19340592-19340611 | MS.gene065266:CDS | 40.0% |
| ATGGTTTCAGGGTCTAAGCA+AGG | + | chr2.4:19340709-19340728 | MS.gene065266:CDS | 45.0% | |
| GCCTTCTCGTTATCATCAGT+TGG | - | chr2.4:19340689-19340708 | None:intergenic | 45.0% | |
| TGGTTTCAGGGTCTAAGCAA+GGG | + | chr2.4:19340710-19340729 | MS.gene065266:CDS | 45.0% | |
| ! | AACGAGAAGGCATGGTTTCA+GGG | + | chr2.4:19340698-19340717 | MS.gene065266:CDS | 45.0% |
| ! | AAGCAAGGGTGCAAAAGCTA+AGG | + | chr2.4:19340724-19340743 | MS.gene065266:CDS | 45.0% |
| ! | CTGATGATAACGAGAAGGCA+TGG | + | chr2.4:19340690-19340709 | MS.gene065266:CDS | 45.0% |
| ! | TAACGAGAAGGCATGGTTTC+AGG | + | chr2.4:19340697-19340716 | MS.gene065266:CDS | 45.0% |
| !! | CAGAAGGGTGTTGCAGATAT+TGG | + | chr2.4:19340575-19340594 | MS.gene065266:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 19340539 | 19340811 | 19340539 | ID=MS.gene065266 |
| chr2.4 | mRNA | 19340539 | 19340811 | 19340539 | ID=MS.gene065266.t1;Parent=MS.gene065266 |
| chr2.4 | exon | 19340539 | 19340811 | 19340539 | ID=MS.gene065266.t1.exon1;Parent=MS.gene065266.t1 |
| chr2.4 | CDS | 19340539 | 19340811 | 19340539 | ID=cds.MS.gene065266.t1;Parent=MS.gene065266.t1 |
| Gene Sequence |
| Protein sequence |
