Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059789.t1 | PNY13417.1 | 53.1 | 254 | 54 | 5 | 1 | 195 | 1 | 248 | 2.40E-50 | 208.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059789.t1 | A0A2K3PDP7 | 53.1 | 254 | 54 | 5 | 1 | 195 | 1 | 248 | 1.7e-50 | 208.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050227 | MS.gene059789 | 0.801607 | 8.31E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene059789 | 0.806977 | 6.33E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene059789 | 0.808992 | 2.36E-50 | -1.69E-46 |
| MS.gene050719 | MS.gene059789 | 0.814451 | 1.53E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene059789 | 0.81129 | 7.54E-51 | -1.69E-46 |
| MS.gene050724 | MS.gene059789 | 0.810464 | 1.14E-50 | -1.69E-46 |
| MS.gene050844 | MS.gene059789 | 0.810965 | 8.87E-51 | -1.69E-46 |
| MS.gene051265 | MS.gene059789 | 0.811805 | 5.83E-51 | -1.69E-46 |
| MS.gene052190 | MS.gene059789 | 0.845449 | 4.03E-59 | -1.69E-46 |
| MS.gene052519 | MS.gene059789 | 0.802047 | 6.75E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene059789 | 0.82092 | 5.32E-53 | -1.69E-46 |
| MS.gene052896 | MS.gene059789 | 0.815779 | 7.76E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene059789 | 0.808331 | 3.26E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene059789 | 0.812773 | 3.58E-51 | -1.69E-46 |
| MS.gene055356 | MS.gene059789 | 0.80736 | 5.25E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene059789 | 0.821633 | 3.64E-53 | -1.69E-46 |
| MS.gene055701 | MS.gene059789 | 0.811342 | 7.35E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene059789 | 0.843078 | 1.75E-58 | -1.69E-46 |
| MS.gene056506 | MS.gene059789 | 0.82228 | 2.58E-53 | -1.69E-46 |
| MS.gene057026 | MS.gene059789 | 0.82974 | 4.35E-55 | -1.69E-46 |
| MS.gene058037 | MS.gene059789 | 0.803324 | 3.68E-49 | -1.69E-46 |
| MS.gene058139 | MS.gene059789 | 0.806324 | 8.69E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene060322 | 0.846203 | 2.52E-59 | -1.69E-46 |
| MS.gene059789 | MS.gene060572 | 0.824947 | 6.12E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene060683 | 0.824441 | 8.06E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene060685 | 0.823462 | 1.37E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene060803 | 0.808325 | 3.27E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene061040 | -0.8156 | 8.51E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene061333 | 0.827566 | 1.46E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene061334 | 0.82024 | 7.62E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene061658 | -0.801125 | 1.04E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene062672 | 0.801764 | 7.72E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene062775 | 0.800585 | 1.34E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene064944 | 0.807048 | 6.11E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene065261 | 0.920772 | 8.38E-88 | -1.69E-46 |
| MS.gene059789 | MS.gene065262 | 0.96083 | 5.29E-119 | -1.69E-46 |
| MS.gene059789 | MS.gene065266 | 0.882856 | 7.33E-71 | -1.69E-46 |
| MS.gene059789 | MS.gene065267 | 0.961038 | 3.06E-119 | -1.69E-46 |
| MS.gene059789 | MS.gene065906 | 0.800139 | 1.66E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene066433 | 0.806924 | 6.49E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene06705 | 0.822479 | 2.32E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene068385 | 0.803837 | 2.88E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene068526 | 0.805566 | 1.25E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene068726 | 0.888284 | 6.77E-73 | -1.69E-46 |
| MS.gene059789 | MS.gene068727 | 0.829445 | 5.13E-55 | -1.69E-46 |
| MS.gene059789 | MS.gene068777 | 0.82284 | 1.91E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene069142 | 0.802868 | 4.57E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene06921 | 0.820542 | 6.49E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene06922 | 0.827626 | 1.41E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene069754 | 0.800502 | 1.40E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene069868 | -0.813249 | 2.82E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene07115 | 0.800489 | 1.41E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene071438 | -0.800982 | 1.12E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene071529 | 0.81078 | 9.72E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene072098 | 0.817995 | 2.47E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene072397 | -0.814849 | 1.25E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene072624 | 0.803735 | 3.03E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene072627 | 0.800086 | 1.70E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene072630 | 0.802133 | 6.48E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene072646 | 0.818163 | 2.26E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene07344 | 0.826615 | 2.46E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene073460 | 0.837621 | 4.67E-57 | -1.69E-46 |
| MS.gene059789 | MS.gene073753 | 0.806159 | 9.42E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene073853 | 0.809356 | 1.97E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene074026 | 0.827224 | 1.76E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene074257 | 0.805238 | 1.47E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene07733 | 0.831462 | 1.65E-55 | -1.69E-46 |
| MS.gene059789 | MS.gene08853 | 0.806915 | 6.52E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene20184 | 0.822184 | 2.71E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene20635 | 0.828184 | 1.04E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene21881 | 0.818504 | 1.89E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene22311 | -0.813043 | 3.13E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene22773 | 0.801758 | 7.74E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene23190 | 0.804585 | 2.01E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene23644 | 0.801096 | 1.06E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene23681 | 0.808118 | 3.62E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene23893 | 0.804033 | 2.62E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene24003 | 0.81491 | 1.21E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene24067 | 0.809719 | 1.65E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene24642 | 0.819662 | 1.03E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene24651 | 0.826721 | 2.32E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene24741 | 0.815842 | 7.52E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene27903 | 0.816457 | 5.48E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene28498 | 0.818537 | 1.86E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene28514 | 0.835015 | 2.15E-56 | -1.69E-46 |
| MS.gene059789 | MS.gene28638 | 0.831817 | 1.35E-55 | -1.69E-46 |
| MS.gene059789 | MS.gene28922 | -0.802116 | 6.54E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene31283 | 0.815503 | 8.94E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene31397 | 0.823687 | 1.21E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene31479 | 0.810569 | 1.08E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene32556 | 0.807091 | 5.99E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene33127 | -0.814035 | 1.89E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene33131 | 0.800294 | 1.54E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene33527 | 0.811349 | 7.32E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene33564 | 0.830903 | 2.26E-55 | -1.69E-46 |
| MS.gene059789 | MS.gene35408 | 0.835877 | 1.30E-56 | -1.69E-46 |
| MS.gene059789 | MS.gene35611 | 0.801086 | 1.06E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene38876 | 0.812248 | 4.67E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene41121 | 0.816168 | 6.35E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene42160 | 0.814082 | 1.85E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene43040 | 0.813059 | 3.10E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene43886 | 0.83702 | 6.65E-57 | -1.69E-46 |
| MS.gene059789 | MS.gene44986 | 0.84739 | 1.19E-59 | -1.69E-46 |
| MS.gene059789 | MS.gene45090 | 0.817477 | 3.23E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene45193 | 0.820824 | 5.59E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene45194 | 0.832839 | 7.52E-56 | -1.69E-46 |
| MS.gene059789 | MS.gene45578 | 0.807948 | 3.94E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene45918 | 0.810188 | 1.30E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene48577 | 0.803041 | 4.21E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene49003 | 0.801647 | 8.16E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene49486 | 0.838993 | 2.07E-57 | -1.69E-46 |
| MS.gene059789 | MS.gene49665 | 0.828716 | 7.70E-55 | -1.69E-46 |
| MS.gene059789 | MS.gene50031 | 0.828841 | 7.18E-55 | -1.69E-46 |
| MS.gene059789 | MS.gene50546 | 0.80005 | 1.73E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene51459 | 0.802516 | 5.41E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene51549 | -0.850314 | 1.84E-60 | -1.69E-46 |
| MS.gene059789 | MS.gene52059 | 0.809732 | 1.64E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene52851 | 0.805102 | 1.57E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene53586 | 0.824238 | 9.00E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene54876 | 0.812623 | 3.86E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene55111 | 0.801373 | 9.29E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene55219 | -0.825711 | 4.04E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene55539 | 0.825565 | 4.37E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene55540 | 0.828114 | 1.08E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene56431 | 0.825827 | 3.79E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene56537 | 0.824733 | 6.88E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene57160 | -0.812217 | 4.74E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene57162 | -0.802628 | 5.13E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene57469 | 0.837905 | 3.94E-57 | -1.69E-46 |
| MS.gene059789 | MS.gene58686 | 0.832581 | 8.71E-56 | -1.69E-46 |
| MS.gene059789 | MS.gene59840 | 0.801411 | 9.12E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene60903 | 0.811527 | 6.70E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene62076 | 0.813662 | 2.29E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene62393 | 0.809728 | 1.64E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene63412 | 0.811242 | 7.72E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene64249 | 0.818192 | 2.23E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene64645 | 0.825198 | 5.34E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene66964 | 0.802811 | 4.70E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene66969 | 0.800003 | 1.77E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene67105 | 0.803149 | 4.00E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene67738 | 0.825533 | 4.45E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene67746 | 0.81097 | 8.84E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene68333 | 0.809051 | 2.29E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene68824 | 0.800709 | 1.27E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene68826 | 0.809397 | 1.93E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene68923 | 0.810009 | 1.43E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene69794 | 0.841262 | 5.29E-58 | -1.69E-46 |
| MS.gene059789 | MS.gene70189 | 0.804004 | 2.66E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene70651 | 0.800973 | 1.12E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene71178 | 0.965182 | 2.83E-124 | -1.69E-46 |
| MS.gene059789 | MS.gene71179 | 0.904609 | 1.03E-79 | -1.69E-46 |
| MS.gene059789 | MS.gene71871 | 0.819551 | 1.09E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene72268 | 0.834399 | 3.07E-56 | -1.69E-46 |
| MS.gene059789 | MS.gene73902 | 0.805562 | 1.26E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene74370 | 0.801643 | 8.17E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene75490 | 0.836347 | 9.87E-57 | -1.69E-46 |
| MS.gene059789 | MS.gene75509 | 0.800609 | 1.33E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene75619 | -0.826564 | 2.53E-54 | -1.69E-46 |
| MS.gene059789 | MS.gene76356 | 0.800604 | 1.33E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene76403 | 0.805198 | 1.50E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene76533 | -0.809186 | 2.14E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene77588 | 0.800487 | 1.41E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene77781 | 0.804849 | 1.77E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene78492 | 0.805072 | 1.59E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene79202 | 0.809185 | 2.14E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene81329 | 0.803174 | 3.95E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene81370 | 0.821523 | 3.86E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene83476 | 0.81304 | 3.13E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene84326 | 0.834621 | 2.70E-56 | -1.69E-46 |
| MS.gene059789 | MS.gene84388 | 0.809248 | 2.08E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene84705 | 0.800502 | 1.40E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene85149 | -0.808015 | 3.81E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene85287 | 0.818855 | 1.58E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene87377 | 0.83151 | 1.60E-55 | -1.69E-46 |
| MS.gene059789 | MS.gene87987 | 0.803134 | 4.03E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene88243 | 0.802719 | 4.91E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene88672 | -0.803089 | 4.12E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene90917 | 0.806122 | 9.59E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene91807 | 0.81085 | 9.39E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene91809 | 0.841402 | 4.86E-58 | -1.69E-46 |
| MS.gene059789 | MS.gene91907 | 0.809643 | 1.71E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene93041 | 0.816237 | 6.13E-52 | -1.69E-46 |
| MS.gene059789 | MS.gene93311 | 0.832988 | 6.90E-56 | -1.69E-46 |
| MS.gene059789 | MS.gene93888 | 0.803813 | 2.91E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene94474 | 0.811652 | 6.29E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene96791 | 0.823312 | 1.48E-53 | -1.69E-46 |
| MS.gene059789 | MS.gene97157 | 0.80282 | 4.68E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene98087 | 0.807727 | 4.39E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene98088 | 0.801073 | 1.07E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene98121 | -0.807697 | 4.45E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene99802 | 0.80707 | 6.05E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059789.t1 | MTR_2g079000 | 63.959 | 197 | 38 | 3 | 1 | 195 | 1 | 166 | 4.38e-71 | 215 |
| MS.gene059789.t1 | MTR_5g012820 | 52.400 | 250 | 55 | 7 | 5 | 191 | 4 | 252 | 3.90e-63 | 199 |
| MS.gene059789.t1 | MTR_5g012820 | 52.400 | 250 | 55 | 7 | 5 | 191 | 14 | 262 | 6.97e-63 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059789.t1 | AT5G05210 | 38.506 | 174 | 66 | 7 | 43 | 191 | 122 | 279 | 1.82e-18 | 82.4 |
| MS.gene059789.t1 | AT5G05210 | 38.506 | 174 | 66 | 7 | 43 | 191 | 130 | 287 | 1.99e-18 | 82.4 |
Find 47 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACGAGAAGGCATGGTTTC+AGG | 0.217653 | 2.4:+19313854 | MS.gene059789:CDS |
| TGAAAGAAAGATAGAGTTTC+CGG | 0.308185 | 2.4:+19314192 | MS.gene059789:CDS |
| AGGTCCTAACTGTGCAAATC+TGG | 0.332531 | 2.4:+19314150 | MS.gene059789:CDS |
| CAGAAGGGTGTTGCAGATAT+TGG | 0.354363 | 2.4:+19313732 | MS.gene059789:CDS |
| GCAGTTAAGCCTTTCATGTC+GGG | 0.372922 | 2.4:+19314049 | MS.gene059789:CDS |
| TGAAAGAGCTAAGAAGTTAG+AGG | 0.400614 | 2.4:+19314330 | MS.gene059789:CDS |
| GCAATAGCTTCACCCTTCTC+AGG | 0.419537 | 2.4:-19314370 | None:intergenic |
| TCTTTAAAAGAGCTTGTGTT+TGG | 0.437000 | 2.4:+19314244 | MS.gene059789:CDS |
| TCCTTTACCACCTTCTCCTC+CGG | 0.440386 | 2.4:-19314211 | None:intergenic |
| ATCTGGAAAATGCTAATAAG+AGG | 0.444036 | 2.4:+19314167 | MS.gene059789:CDS |
| TGTCCTTCTCAAAGAATGCT+TGG | 0.450243 | 2.4:+19313988 | MS.gene059789:intron |
| GTTGGTAGATAAAACTTAGC+TGG | 0.463279 | 2.4:-19313825 | None:intergenic |
| AACGAGAAGGCATGGTTTCA+GGG | 0.469679 | 2.4:+19313855 | MS.gene059789:CDS |
| GTCCTTCTCAAAGAATGCTT+GGG | 0.470258 | 2.4:+19313989 | MS.gene059789:intron |
| TCTTTCAAGTTCCTTGTGCT+TGG | 0.476271 | 2.4:-19314314 | None:intergenic |
| GGCAATGTTAAACTTCAGAA+TGG | 0.487362 | 2.4:+19314265 | MS.gene059789:CDS |
| GATAGAGTTTCCGGAGGAGA+AGG | 0.496889 | 2.4:+19314201 | MS.gene059789:CDS |
| TATTGGTGCTTGTCAAGACT+TGG | 0.497123 | 2.4:+19313749 | MS.gene059789:CDS |
| AGGGTGAAGCTATTGCTAAA+AGG | 0.503520 | 2.4:+19314377 | MS.gene059789:CDS |
| TCCGGAGGAGAAGGTGGTAA+AGG | 0.513098 | 2.4:+19314210 | MS.gene059789:CDS |
| GCCTTCTCGTTATCATCAGT+TGG | 0.521411 | 2.4:-19313843 | None:intergenic |
| AAACTTGAAGAGTTTCAGTC+AGG | 0.526291 | 2.4:+19314130 | MS.gene059789:CDS |
| GGAGATTAAGAAGAAGCAGA+AGG | 0.532482 | 2.4:+19313716 | MS.gene059789:CDS |
| TAAGCCTTTCATGTCGGGGT+TGG | 0.538275 | 2.4:+19314054 | MS.gene059789:CDS |
| TGCAGTTAAGCCTTTCATGT+CGG | 0.538494 | 2.4:+19314048 | MS.gene059789:CDS |
| GAGATTAAGAAGAAGCAGAA+GGG | 0.542455 | 2.4:+19313717 | MS.gene059789:CDS |
| CCTTTCGCGTAGTTCTTCAA+AGG | 0.546785 | 2.4:-19314098 | None:intergenic |
| GCTATTGCTAAAAGGAAGCA+TGG | 0.549443 | 2.4:+19314385 | MS.gene059789:CDS |
| GCTTCCAACCCCGACATGAA+AGG | 0.556004 | 2.4:-19314058 | None:intergenic |
| CGGGGTTGGAAGCAGATGAT+CGG | 0.561569 | 2.4:+19314068 | MS.gene059789:CDS |
| CTTCAGAATGGTGAAATGCA+AGG | 0.562609 | 2.4:+19314277 | MS.gene059789:CDS |
| TTCAGAATGGTGAAATGCAA+GGG | 0.568164 | 2.4:+19314278 | MS.gene059789:CDS |
| AGAGTTTCCGGAGGAGAAGG+TGG | 0.570130 | 2.4:+19314204 | MS.gene059789:CDS |
| AATGACAGCGATGGAGAGGA+AGG | 0.580649 | 2.4:+19314025 | MS.gene059789:CDS |
| AATGAATGACAGCGATGGAG+AGG | 0.585376 | 2.4:+19314021 | MS.gene059789:CDS |
| GAAGAAGAATGATCCTGAGA+AGG | 0.589096 | 2.4:+19314357 | MS.gene059789:CDS |
| AAGAAGAATGATCCTGAGAA+GGG | 0.591026 | 2.4:+19314358 | MS.gene059789:CDS |
| ATGGTTTCAGGGTCTAAGCA+AGG | 0.591717 | 2.4:+19313866 | MS.gene059789:CDS |
| CAGTTAAGCCTTTCATGTCG+GGG | 0.594657 | 2.4:+19314050 | MS.gene059789:CDS |
| TCTGGAAAATGCTAATAAGA+GGG | 0.594774 | 2.4:+19314168 | MS.gene059789:CDS |
| CTGATGATAACGAGAAGGCA+TGG | 0.624704 | 2.4:+19313847 | MS.gene059789:CDS |
| CCTTTGAAGAACTACGCGAA+AGG | 0.632563 | 2.4:+19314098 | MS.gene059789:CDS |
| TGGTTTCAGGGTCTAAGCAA+GGG | 0.633440 | 2.4:+19313867 | MS.gene059789:CDS |
| GAGAAAAGTTTCCAAGCACA+AGG | 0.645702 | 2.4:+19314303 | MS.gene059789:CDS |
| GGAGGAGAAGGTGGTAAAGG+AGG | 0.646856 | 2.4:+19314213 | MS.gene059789:CDS |
| AAGAAAGATAGAGTTTCCGG+AGG | 0.655190 | 2.4:+19314195 | MS.gene059789:CDS |
| ACCAACTGATGATAACGAGA+AGG | 0.659759 | 2.4:+19313842 | MS.gene059789:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAGACATATTATGAAGTCT+CGG | + | chr2.4:19313918-19313937 | MS.gene059789:intron | 25.0% |
| ! | GATCAATTTGTCGAAAAAAT+GGG | - | chr2.4:19313796-19313815 | None:intergenic | 25.0% |
| ATCTGGAAAATGCTAATAAG+AGG | + | chr2.4:19314167-19314186 | MS.gene059789:CDS | 30.0% | |
| CGATCAATTTGTCGAAAAAA+TGG | - | chr2.4:19313797-19313816 | None:intergenic | 30.0% | |
| TCTGGAAAATGCTAATAAGA+GGG | + | chr2.4:19314168-19314187 | MS.gene059789:CDS | 30.0% | |
| TGAAAGAAAGATAGAGTTTC+CGG | + | chr2.4:19314192-19314211 | MS.gene059789:CDS | 30.0% | |
| ! | TCTTTAAAAGAGCTTGTGTT+TGG | + | chr2.4:19314244-19314263 | MS.gene059789:CDS | 30.0% |
| AAACTTGAAGAGTTTCAGTC+AGG | + | chr2.4:19314130-19314149 | MS.gene059789:CDS | 35.0% | |
| AAGAAGAATGATCCTGAGAA+GGG | + | chr2.4:19314358-19314377 | MS.gene059789:CDS | 35.0% | |
| GAAAAAATGAATGACAGCGA+TGG | + | chr2.4:19314016-19314035 | MS.gene059789:CDS | 35.0% | |
| GAGATTAAGAAGAAGCAGAA+GGG | + | chr2.4:19313717-19313736 | MS.gene059789:CDS | 35.0% | |
| GGCAATGTTAAACTTCAGAA+TGG | + | chr2.4:19314265-19314284 | MS.gene059789:CDS | 35.0% | |
| TGAAAGAGCTAAGAAGTTAG+AGG | + | chr2.4:19314330-19314349 | MS.gene059789:CDS | 35.0% | |
| TTCAGAATGGTGAAATGCAA+GGG | + | chr2.4:19314278-19314297 | MS.gene059789:CDS | 35.0% | |
| ! | GTTGGTAGATAAAACTTAGC+TGG | - | chr2.4:19313828-19313847 | None:intergenic | 35.0% |
| ! | TTTTCCAGATTTGCACAGTT+AGG | - | chr2.4:19314157-19314176 | None:intergenic | 35.0% |
| !! | AAGCTCTTTTAAAGACTCTG+TGG | - | chr2.4:19314239-19314258 | None:intergenic | 35.0% |
| AAGAAAGATAGAGTTTCCGG+AGG | + | chr2.4:19314195-19314214 | MS.gene059789:CDS | 40.0% | |
| AGGGTGAAGCTATTGCTAAA+AGG | + | chr2.4:19314377-19314396 | MS.gene059789:CDS | 40.0% | |
| CTTCAGAATGGTGAAATGCA+AGG | + | chr2.4:19314277-19314296 | MS.gene059789:CDS | 40.0% | |
| GAAGAAGAATGATCCTGAGA+AGG | + | chr2.4:19314357-19314376 | MS.gene059789:CDS | 40.0% | |
| GAGAAAAGTTTCCAAGCACA+AGG | + | chr2.4:19314303-19314322 | MS.gene059789:CDS | 40.0% | |
| GCTATTGCTAAAAGGAAGCA+TGG | + | chr2.4:19314385-19314404 | MS.gene059789:CDS | 40.0% | |
| GGAGATTAAGAAGAAGCAGA+AGG | + | chr2.4:19313716-19313735 | MS.gene059789:CDS | 40.0% | |
| GTCCTTCTCAAAGAATGCTT+GGG | + | chr2.4:19313989-19314008 | MS.gene059789:intron | 40.0% | |
| GTTAGGGTCTGAACTCTAAA+CGG | - | chr2.4:19313949-19313968 | None:intergenic | 40.0% | |
| TGCAGTTAAGCCTTTCATGT+CGG | + | chr2.4:19314048-19314067 | MS.gene059789:CDS | 40.0% | |
| TGTCCTTCTCAAAGAATGCT+TGG | + | chr2.4:19313988-19314007 | MS.gene059789:intron | 40.0% | |
| ! | AAGCATTCTTTGAGAAGGAC+AGG | - | chr2.4:19313989-19314008 | None:intergenic | 40.0% |
| ! | ACCAACTGATGATAACGAGA+AGG | + | chr2.4:19313842-19313861 | MS.gene059789:CDS | 40.0% |
| ! | ATTCTTTGAGAAGGACAGGA+TGG | - | chr2.4:19313985-19314004 | None:intergenic | 40.0% |
| ! | TATTGGTGCTTGTCAAGACT+TGG | + | chr2.4:19313749-19313768 | MS.gene059789:CDS | 40.0% |
| ! | TTCCCAAGCATTCTTTGAGA+AGG | - | chr2.4:19313994-19314013 | None:intergenic | 40.0% |
| !! | TCTTTCAAGTTCCTTGTGCT+TGG | - | chr2.4:19314317-19314336 | None:intergenic | 40.0% |
| AATGAATGACAGCGATGGAG+AGG | + | chr2.4:19314021-19314040 | MS.gene059789:CDS | 45.0% | |
| AGGTCCTAACTGTGCAAATC+TGG | + | chr2.4:19314150-19314169 | MS.gene059789:CDS | 45.0% | |
| ATGGTTTCAGGGTCTAAGCA+AGG | + | chr2.4:19313866-19313885 | MS.gene059789:CDS | 45.0% | |
| CAGTTAAGCCTTTCATGTCG+GGG | + | chr2.4:19314050-19314069 | MS.gene059789:CDS | 45.0% | |
| CCTTTCGCGTAGTTCTTCAA+AGG | - | chr2.4:19314101-19314120 | None:intergenic | 45.0% | |
| GCAGTTAAGCCTTTCATGTC+GGG | + | chr2.4:19314049-19314068 | MS.gene059789:CDS | 45.0% | |
| GCCTTCTCGTTATCATCAGT+TGG | - | chr2.4:19313846-19313865 | None:intergenic | 45.0% | |
| TGGTTTCAGGGTCTAAGCAA+GGG | + | chr2.4:19313867-19313886 | MS.gene059789:CDS | 45.0% | |
| ! | AACGAGAAGGCATGGTTTCA+GGG | + | chr2.4:19313855-19313874 | MS.gene059789:CDS | 45.0% |
| ! | AAGCAAGGGTGCAAAAGCTA+AGG | + | chr2.4:19313881-19313900 | MS.gene059789:intron | 45.0% |
| ! | CCTTTGAAGAACTACGCGAA+AGG | + | chr2.4:19314098-19314117 | MS.gene059789:CDS | 45.0% |
| ! | CTGATGATAACGAGAAGGCA+TGG | + | chr2.4:19313847-19313866 | MS.gene059789:CDS | 45.0% |
| ! | TAACGAGAAGGCATGGTTTC+AGG | + | chr2.4:19313854-19313873 | MS.gene059789:CDS | 45.0% |
| !! | ATGGTTGCAGTTGGCTTGTT+AGG | - | chr2.4:19313966-19313985 | None:intergenic | 45.0% |
| !! | CAGAAGGGTGTTGCAGATAT+TGG | + | chr2.4:19313732-19313751 | MS.gene059789:CDS | 45.0% |
| !! | TGGTTGCAGTTGGCTTGTTA+GGG | - | chr2.4:19313965-19313984 | None:intergenic | 45.0% |
| AAGGACAGGATGGTTGCAGT+TGG | - | chr2.4:19313975-19313994 | None:intergenic | 50.0% | |
| AATGACAGCGATGGAGAGGA+AGG | + | chr2.4:19314025-19314044 | MS.gene059789:CDS | 50.0% | |
| GATAGAGTTTCCGGAGGAGA+AGG | + | chr2.4:19314201-19314220 | MS.gene059789:CDS | 50.0% | |
| GCAATAGCTTCACCCTTCTC+AGG | - | chr2.4:19314373-19314392 | None:intergenic | 50.0% | |
| TAAGCCTTTCATGTCGGGGT+TGG | + | chr2.4:19314054-19314073 | MS.gene059789:CDS | 50.0% | |
| TCCTTTACCACCTTCTCCTC+CGG | - | chr2.4:19314214-19314233 | None:intergenic | 50.0% | |
| AGAGTTTCCGGAGGAGAAGG+TGG | + | chr2.4:19314204-19314223 | MS.gene059789:CDS | 55.0% | |
| CGGGGTTGGAAGCAGATGAT+CGG | + | chr2.4:19314068-19314087 | MS.gene059789:CDS | 55.0% | |
| GCTTCCAACCCCGACATGAA+AGG | - | chr2.4:19314061-19314080 | None:intergenic | 55.0% | |
| GGAGGAGAAGGTGGTAAAGG+AGG | + | chr2.4:19314213-19314232 | MS.gene059789:CDS | 55.0% | |
| TCCGGAGGAGAAGGTGGTAA+AGG | + | chr2.4:19314210-19314229 | MS.gene059789:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 19313714 | 19314420 | 19313714 | ID=MS.gene059789 |
| chr2.4 | mRNA | 19313714 | 19314420 | 19313714 | ID=MS.gene059789.t1;Parent=MS.gene059789 |
| chr2.4 | exon | 19313714 | 19313888 | 19313714 | ID=MS.gene059789.t1.exon1;Parent=MS.gene059789.t1 |
| chr2.4 | CDS | 19313714 | 19313888 | 19313714 | ID=cds.MS.gene059789.t1;Parent=MS.gene059789.t1 |
| chr2.4 | exon | 19314002 | 19314420 | 19314002 | ID=MS.gene059789.t1.exon2;Parent=MS.gene059789.t1 |
| chr2.4 | CDS | 19314002 | 19314420 | 19314002 | ID=cds.MS.gene059789.t1;Parent=MS.gene059789.t1 |
| Gene Sequence |
| Protein sequence |
