Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065356.t1 | XP_003625486.1 | 100 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.70E-21 | 111.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065356.t1 | Q2HT99 | 100.0 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.0e-21 | 111.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050584 | MS.gene065356 | 0.817418 | 3.33E-52 | -1.69E-46 |
| MS.gene059006 | MS.gene065356 | 0.818575 | 1.82E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065356.t1 | MTR_7g099670 | 98.077 | 104 | 2 | 0 | 1 | 104 | 1 | 104 | 3.16e-60 | 179 |
| MS.gene065356.t1 | MTR_1g069085 | 68.000 | 50 | 14 | 2 | 1 | 49 | 1 | 49 | 5.67e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGGTGGACTTGGTGGTTT+GGG | 0.209445 | 7.4:-10597162 | MS.gene065356:CDS |
| ACATTTGCTGGTGGTTATTC+AGG | 0.231052 | 7.4:-10597258 | MS.gene065356:CDS |
| GGAGGACTTGGTGGGGTTGG+TGG | 0.235350 | 7.4:-10597141 | MS.gene065356:CDS |
| CCTAGGTGGACTTGGTGGTT+TGG | 0.244833 | 7.4:-10597163 | MS.gene065356:CDS |
| CTTGGTGGTTTGGGAGGACT+TGG | 0.272357 | 7.4:-10597153 | MS.gene065356:CDS |
| GGACTTGGTGGGGTTGGTGG+TGG | 0.293034 | 7.4:-10597138 | MS.gene065356:CDS |
| GGTGGAGCTGGTGGTGGTGT+TGG | 0.293326 | 7.4:-10597120 | MS.gene065356:CDS |
| GGTGGAATCGGTGGTGATTT+AGG | 0.312961 | 7.4:-10597198 | MS.gene065356:CDS |
| GGTGGTTTGGGAGGACTTGG+TGG | 0.314590 | 7.4:-10597150 | MS.gene065356:CDS |
| TTGGGAGGACTTGGTGGGGT+TGG | 0.314725 | 7.4:-10597144 | MS.gene065356:CDS |
| GGTGGGGTTGGTGGTGGAGC+TGG | 0.316689 | 7.4:-10597132 | MS.gene065356:CDS |
| GTTGGTGGTGGAGCTGGTGG+TGG | 0.321018 | 7.4:-10597126 | MS.gene065356:CDS |
| GGAAACAATGGACTTCCTTT+TGG | 0.322482 | 7.4:-10597231 | MS.gene065356:CDS |
| TTAGGTGGTGGTGGTGGCCT+AGG | 0.323708 | 7.4:-10597180 | MS.gene065356:CDS |
| GGTGGTGGCCTAGGTGGACT+TGG | 0.331166 | 7.4:-10597171 | MS.gene065356:CDS |
| GGTGGTGTTGGAGGTGGTGT+TGG | 0.331290 | 7.4:-10597108 | MS.gene065356:CDS |
| GGGGTTGGTGGTGGAGCTGG+TGG | 0.333006 | 7.4:-10597129 | MS.gene065356:CDS |
| GCTGGTGGTTATTCAGGAAT+AGG | 0.341133 | 7.4:-10597252 | MS.gene065356:CDS |
| GTTGGAGGTGGTGTTGGAAC+TGG | 0.344227 | 7.4:-10597102 | MS.gene065356:CDS |
| CAAATGTCATGAAGTTCTTC+TGG | 0.354882 | 7.4:+10597274 | None:intergenic |
| GCTGGTGGTGGTGTTGGAGG+TGG | 0.373109 | 7.4:-10597114 | MS.gene065356:CDS |
| AAGAACTTCATGACATTTGC+TGG | 0.375624 | 7.4:-10597270 | MS.gene065356:CDS |
| GGAGCTGGTGGTGGTGTTGG+AGG | 0.379910 | 7.4:-10597117 | MS.gene065356:CDS |
| GGTGGTGTTGGAACTGGTGT+TGG | 0.385912 | 7.4:-10597096 | MS.gene065356:CDS |
| GGTGGAATTGGAGGTGGAAT+CGG | 0.386910 | 7.4:-10597210 | MS.gene065356:CDS |
| GTGGTTTGGGAGGACTTGGT+GGG | 0.431797 | 7.4:-10597149 | MS.gene065356:CDS |
| TGGTTTGGGAGGACTTGGTG+GGG | 0.448017 | 7.4:-10597148 | MS.gene065356:CDS |
| GGTGTTGGAACTGGTGTTGG+TGG | 0.458137 | 7.4:-10597093 | MS.gene065356:CDS |
| AAAGTGGTGTATGTTGTTGT+TGG | 0.463389 | 7.4:-10597361 | MS.gene065356:CDS |
| GGTGGCCTAGGTGGACTTGG+TGG | 0.480801 | 7.4:-10597168 | MS.gene065356:CDS |
| GGTGGACTTGGTGGTTTGGG+AGG | 0.508576 | 7.4:-10597159 | MS.gene065356:CDS |
| GTTGGTTCTTGCTCTTGTTG+TGG | 0.516173 | 7.4:-10597343 | MS.gene065356:CDS |
| GGTGGTGGTGGTGGCCTAGG+TGG | 0.516203 | 7.4:-10597177 | MS.gene065356:CDS |
| ACCAACTGATGCTGGTTTGA+AGG | 0.518003 | 7.4:-10597298 | MS.gene065356:CDS |
| GGTGATTTAGGTGGTGGTGG+TGG | 0.527759 | 7.4:-10597186 | MS.gene065356:CDS |
| ATCGGTGGTGATTTAGGTGG+TGG | 0.527781 | 7.4:-10597192 | MS.gene065356:CDS |
| CCACCTCCAATTCCACCAAA+AGG | 0.542402 | 7.4:+10597216 | None:intergenic |
| GGAATTGGAGGTGGAATCGG+TGG | 0.552447 | 7.4:-10597207 | MS.gene065356:CDS |
| GGTGGTGATTTAGGTGGTGG+TGG | 0.559090 | 7.4:-10597189 | MS.gene065356:CDS |
| GAATACTGATCTTAAGGGAA+AGG | 0.564229 | 7.4:+10597063 | None:intergenic |
| AGAAATGTACCAACTGATGC+TGG | 0.575990 | 7.4:-10597306 | MS.gene065356:CDS |
| TATTCAGGAATAGGAAACAA+TGG | 0.605549 | 7.4:-10597243 | MS.gene065356:CDS |
| GGAATCGGTGGTGATTTAGG+TGG | 0.612801 | 7.4:-10597195 | MS.gene065356:CDS |
| GAGTGAGAGATATGGCAAAG+TGG | 0.614546 | 7.4:-10597377 | None:intergenic |
| AACTTCATGACATTTGCTGG+TGG | 0.621619 | 7.4:-10597267 | MS.gene065356:CDS |
| TCCTTCAAACCAGCATCAGT+TGG | 0.622405 | 7.4:+10597297 | None:intergenic |
| CCAAACCACCAAGTCCACCT+AGG | 0.637542 | 7.4:+10597163 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTCAGGAATAGGAAACAA+TGG | - | chr7.4:10597197-10597216 | MS.gene065356:CDS | 30.0% | |
| AAAGTGGTGTATGTTGTTGT+TGG | - | chr7.4:10597079-10597098 | MS.gene065356:CDS | 35.0% | |
| AAGAACTTCATGACATTTGC+TGG | - | chr7.4:10597170-10597189 | MS.gene065356:CDS | 35.0% | |
| CAAATGTCATGAAGTTCTTC+TGG | + | chr7.4:10597169-10597188 | None:intergenic | 35.0% | |
| AGAAATGTACCAACTGATGC+TGG | - | chr7.4:10597134-10597153 | MS.gene065356:CDS | 40.0% | |
| ! | AACTTCATGACATTTGCTGG+TGG | - | chr7.4:10597173-10597192 | MS.gene065356:CDS | 40.0% |
| ! | ACATTTGCTGGTGGTTATTC+AGG | - | chr7.4:10597182-10597201 | MS.gene065356:CDS | 40.0% |
| !! | AACAATGGACTTCCTTTTGG+TGG | - | chr7.4:10597212-10597231 | MS.gene065356:CDS | 40.0% |
| !! | GGAAACAATGGACTTCCTTT+TGG | - | chr7.4:10597209-10597228 | MS.gene065356:CDS | 40.0% |
| GCTGGTGGTTATTCAGGAAT+AGG | - | chr7.4:10597188-10597207 | MS.gene065356:CDS | 45.0% | |
| TCCTTCAAACCAGCATCAGT+TGG | + | chr7.4:10597146-10597165 | None:intergenic | 45.0% | |
| ! | CTTCCTTTTGGTGGAATTGG+AGG | - | chr7.4:10597221-10597240 | MS.gene065356:CDS | 45.0% |
| ! | GGACTTCCTTTTGGTGGAAT+TGG | - | chr7.4:10597218-10597237 | MS.gene065356:CDS | 45.0% |
| ! | GTTGGTTCTTGCTCTTGTTG+TGG | - | chr7.4:10597097-10597116 | MS.gene065356:CDS | 45.0% |
| !! | ACCAACTGATGCTGGTTTGA+AGG | - | chr7.4:10597142-10597161 | MS.gene065356:CDS | 45.0% |
| ATCGGTGGTGATTTAGGTGG+TGG | - | chr7.4:10597248-10597267 | MS.gene065356:CDS | 50.0% | |
| CCACCTCCAATTCCACCAAA+AGG | + | chr7.4:10597227-10597246 | None:intergenic | 50.0% | |
| GGAATCGGTGGTGATTTAGG+TGG | - | chr7.4:10597245-10597264 | MS.gene065356:CDS | 50.0% | |
| GGTGGAATCGGTGGTGATTT+AGG | - | chr7.4:10597242-10597261 | MS.gene065356:CDS | 50.0% | |
| GGTGGAATTGGAGGTGGAAT+CGG | - | chr7.4:10597230-10597249 | MS.gene065356:CDS | 50.0% | |
| ! | CCTTTTGGTGGAATTGGAGG+TGG | - | chr7.4:10597224-10597243 | MS.gene065356:CDS | 50.0% |
| !! | CTAGGTGGACTTGGTGGTTT+GGG | - | chr7.4:10597278-10597297 | MS.gene065356:CDS | 50.0% |
| CCAAACCACCAAGTCCACCT+AGG | + | chr7.4:10597280-10597299 | None:intergenic | 55.0% | |
| GGAATTGGAGGTGGAATCGG+TGG | - | chr7.4:10597233-10597252 | MS.gene065356:CDS | 55.0% | |
| ! | CTTGGTGGTTTGGGAGGACT+TGG | - | chr7.4:10597287-10597306 | MS.gene065356:CDS | 55.0% |
| ! | GGTGGTGATTTAGGTGGTGG+TGG | - | chr7.4:10597251-10597270 | MS.gene065356:CDS | 55.0% |
| ! | GGTGGTGTTGGAACTGGTGT+TGG | - | chr7.4:10597344-10597363 | MS.gene065356:CDS | 55.0% |
| ! | GGTGTTGGAACTGGTGTTGG+TGG | - | chr7.4:10597347-10597366 | MS.gene065356:CDS | 55.0% |
| ! | GTGGTTTGGGAGGACTTGGT+GGG | - | chr7.4:10597291-10597310 | MS.gene065356:CDS | 55.0% |
| ! | GTTGGAGGTGGTGTTGGAAC+TGG | - | chr7.4:10597338-10597357 | MS.gene065356:CDS | 55.0% |
| !! | CCTAGGTGGACTTGGTGGTT+TGG | - | chr7.4:10597277-10597296 | MS.gene065356:CDS | 55.0% |
| !! | GGTGATTTAGGTGGTGGTGG+TGG | - | chr7.4:10597254-10597273 | MS.gene065356:CDS | 55.0% |
| !! | TGGTTTGGGAGGACTTGGTG+GGG | - | chr7.4:10597292-10597311 | MS.gene065356:CDS | 55.0% |
| ! | GGTGGTGTTGGAGGTGGTGT+TGG | - | chr7.4:10597332-10597351 | MS.gene065356:CDS | 60.0% |
| ! | GGTGGTTTGGGAGGACTTGG+TGG | - | chr7.4:10597290-10597309 | MS.gene065356:CDS | 60.0% |
| !! | GGTGGACTTGGTGGTTTGGG+AGG | - | chr7.4:10597281-10597300 | MS.gene065356:CDS | 60.0% |
| !! | TTAGGTGGTGGTGGTGGCCT+AGG | - | chr7.4:10597260-10597279 | MS.gene065356:CDS | 60.0% |
| !! | TTGGGAGGACTTGGTGGGGT+TGG | - | chr7.4:10597296-10597315 | MS.gene065356:CDS | 60.0% |
| ! | GGTGGCCTAGGTGGACTTGG+TGG | - | chr7.4:10597272-10597291 | MS.gene065356:CDS | 65.0% |
| ! | GGTGGTGGCCTAGGTGGACT+TGG | - | chr7.4:10597269-10597288 | MS.gene065356:CDS | 65.0% |
| !! | GCTGGTGGTGGTGTTGGAGG+TGG | - | chr7.4:10597326-10597345 | MS.gene065356:CDS | 65.0% |
| !! | GGACTTGGTGGGGTTGGTGG+TGG | - | chr7.4:10597302-10597321 | MS.gene065356:CDS | 65.0% |
| !! | GGAGCTGGTGGTGGTGTTGG+AGG | - | chr7.4:10597323-10597342 | MS.gene065356:CDS | 65.0% |
| !! | GGAGGACTTGGTGGGGTTGG+TGG | - | chr7.4:10597299-10597318 | MS.gene065356:CDS | 65.0% |
| !! | GGTGGAGCTGGTGGTGGTGT+TGG | - | chr7.4:10597320-10597339 | MS.gene065356:CDS | 65.0% |
| !! | GTTGGTGGTGGAGCTGGTGG+TGG | - | chr7.4:10597314-10597333 | MS.gene065356:CDS | 65.0% |
| !! | GGGGTTGGTGGTGGAGCTGG+TGG | - | chr7.4:10597311-10597330 | MS.gene065356:CDS | 70.0% |
| !! | GGTGGGGTTGGTGGTGGAGC+TGG | - | chr7.4:10597308-10597327 | MS.gene065356:CDS | 70.0% |
| !! | GGTGGTGGTGGTGGCCTAGG+TGG | - | chr7.4:10597263-10597282 | MS.gene065356:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10597074 | 10597388 | 10597074 | ID=MS.gene065356 |
| chr7.4 | mRNA | 10597074 | 10597388 | 10597074 | ID=MS.gene065356.t1;Parent=MS.gene065356 |
| chr7.4 | exon | 10597074 | 10597388 | 10597074 | ID=MS.gene065356.t1.exon1;Parent=MS.gene065356.t1 |
| chr7.4 | CDS | 10597074 | 10597388 | 10597074 | ID=cds.MS.gene065356.t1;Parent=MS.gene065356.t1 |
| Gene Sequence |
| Protein sequence |
