AlfalfaGEDB | MS.gene06538

M. sativa cultivar XinJiangDaYe / MS.gene06538

KEGG: K14492
GO: GO:0000160 GO:0007165 GO:0008150 GO:0009987 GO:0035556 GO:0050789 GO:0050794 GO:0065007
PFAM: PF00072.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06538.t1	XP_013461496.1	92.4	157	9	2	1	155	1	156	1.00E-72	282.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06538.t1	Q9FPR6	72.3	130	36	0	19	148	21	150	6.3e-47	188.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06538.t1	A0A072V1G2	92.4	157	9	2	1	155	1	156	7.3e-73	282.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene06538.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049408	MS.gene06538	0.981158	6.51E-152	-1.69E-46
MS.gene050584	MS.gene06538	0.811897	5.56E-51	-1.69E-46
MS.gene051789	MS.gene06538	0.821718	3.48E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06538.t1	MTR_3g093860	92.357	157	9	2	1	155	1	156	2.73e-103	292
MS.gene06538.t1	MTR_1g049100	60.448	134	52	1	14	146	4	137	6.02e-55	171
MS.gene06538.t1	MTR_3g078613	60.769	130	50	1	19	148	24	152	9.77e-55	171
MS.gene06538.t1	MTR_3g078613	61.069	131	49	2	19	148	24	153	1.79e-53	168
MS.gene06538.t1	MTR_5g036480	60.606	132	47	2	19	146	17	147	4.98e-52	165
MS.gene06538.t1	MTR_8g038620	57.353	136	51	1	19	147	10	145	1.11e-51	162
MS.gene06538.t1	MTR_4g106590	60.305	131	50	2	19	148	27	156	1.64e-51	163
MS.gene06538.t1	MTR_7g490310	59.091	132	46	2	19	142	10	141	1.52e-50	162
MS.gene06538.t1	MTR_3g088630	53.191	141	48	2	19	142	23	162	6.96e-46	150
MS.gene06538.t1	MTR_3g015490	52.143	140	50	3	19	147	5	138	5.93e-43	140
MS.gene06538.t1	MTR_6g007460	40.659	182	54	3	19	147	12	192	6.62e-37	126
MS.gene06538.t1	MTR_2g050020	91.379	58	4	1	1	58	35	91	9.71e-31	107
MS.gene06538.t1	MTR_4g051330	56.863	51	22	0	97	147	77	127	3.71e-16	71.2
MS.gene06538.t1	MTR_4g061360	34.959	123	70	1	20	142	91	203	8.56e-16	73.9
MS.gene06538.t1	MTR_1g067110	34.959	123	70	1	20	142	87	199	6.41e-15	71.2
MS.gene06538.t1	MTR_1g067110	34.959	123	70	1	20	142	87	199	7.80e-15	70.9
MS.gene06538.t1	MTR_3g092780	30.645	124	74	2	20	142	58	170	7.26e-14	68.2
MS.gene06538.t1	MTR_3g092780	30.645	124	74	2	20	142	58	170	7.27e-14	68.2
MS.gene06538.t1	MTR_3g092780	30.645	124	74	2	20	142	58	170	7.41e-14	68.2
MS.gene06538.t1	MTR_3g092780	30.645	124	74	2	20	142	58	170	7.70e-14	68.2
MS.gene06538.t1	MTR_8g024260	30.894	123	75	1	20	142	38	150	3.14e-13	66.2
MS.gene06538.t1	MTR_7g118260	30.894	123	75	1	20	142	16	128	4.29e-13	65.9
MS.gene06538.t1	MTR_7g118260	30.894	123	75	1	20	142	16	128	5.02e-13	65.9
MS.gene06538.t1	MTR_8g079940	28.906	128	78	2	20	147	31	145	9.94e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06538.t1	AT3G56380	72.308	130	36	0	19	148	21	150	2.34e-64	194
MS.gene06538.t1	AT3G56380	72.519	131	35	1	19	148	21	151	4.69e-63	191
MS.gene06538.t1	AT2G40670	70.896	134	33	2	19	148	30	161	7.07e-63	191
MS.gene06538.t1	AT2G40670	71.111	135	32	3	19	148	30	162	1.45e-61	187
MS.gene06538.t1	AT1G10470	61.719	128	48	1	19	146	21	147	3.30e-53	169
MS.gene06538.t1	AT1G59940	58.647	133	54	1	14	146	29	160	4.81e-53	168
MS.gene06538.t1	AT1G10470	61.719	128	48	1	19	146	35	161	6.13e-53	168
MS.gene06538.t1	AT1G59940	58.647	133	54	1	14	146	121	252	7.17e-52	167
MS.gene06538.t1	AT3G48100	57.364	129	55	0	19	147	26	154	1.29e-51	162
MS.gene06538.t1	AT5G62920	58.955	134	54	1	19	152	26	158	3.10e-51	162
MS.gene06538.t1	AT3G57040	53.623	138	55	1	19	147	10	147	6.22e-51	162
MS.gene06538.t1	AT3G57040	53.623	138	55	1	19	147	10	147	6.22e-51	162
MS.gene06538.t1	AT2G41310	51.724	145	63	2	10	147	1	145	2.24e-50	161
MS.gene06538.t1	AT1G74890	59.091	132	53	1	19	150	19	149	5.77e-50	159
MS.gene06538.t1	AT1G19050	58.015	131	52	2	19	148	25	153	3.33e-49	157
MS.gene06538.t1	AT1G10470	50.000	74	33	3	75	146	14	85	3.10e-15	69.7
MS.gene06538.t1	AT5G24470	31.452	124	73	2	20	142	52	164	6.86e-14	68.2
MS.gene06538.t1	AT5G02810	31.707	123	74	1	20	142	80	192	2.07e-12	64.3
MS.gene06538.t1	AT5G60100	33.333	123	72	1	20	142	66	178	2.24e-12	63.9
MS.gene06538.t1	AT5G60100	33.333	123	72	1	20	142	66	178	2.24e-12	63.9
MS.gene06538.t1	AT5G60100	33.333	123	72	1	20	142	66	178	2.24e-12	63.9
MS.gene06538.t1	AT5G60100	33.333	123	72	1	20	142	66	178	2.25e-12	63.9
MS.gene06538.t1	AT5G60100	33.333	123	72	1	20	142	66	178	5.14e-12	62.8
MS.gene06538.t1	AT5G60100	33.333	123	72	1	20	142	66	178	5.14e-12	62.8
MS.gene06538.t1	AT5G60100	33.333	123	72	1	20	142	66	178	5.14e-12	62.8
MS.gene06538.t1	AT2G46790	28.226	124	79	1	19	142	38	151	1.85e-11	61.2
MS.gene06538.t1	AT2G46790	28.226	124	79	1	19	142	38	151	2.23e-11	61.2
MS.gene06538.t1	AT5G07210	30.081	123	72	3	20	142	18	126	3.21e-11	60.8

Find 39 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGAAGGCATTGGAGTATT+TGG	0.158019	3.2:-78715107	MS.gene06538:CDS
GACAGGCCAGCTGATGAATT+TGG	0.201243	3.2:-78714486	MS.gene06538:CDS
ACAGTGACAACTGCTGAAAA+TGG	0.248549	3.2:-78715130	MS.gene06538:intron
CTGAAGGCATTGGAGTATTT+GGG	0.249714	3.2:-78715106	MS.gene06538:CDS
GTAGGGACCCAAAGTGAATT+TGG	0.331645	3.2:-78714910	MS.gene06538:intron
AATTACTTGTTGATCCTTGT+TGG	0.359191	3.2:+78714662	None:intergenic
TCAAAGTGGGTGATGGAAAA+TGG	0.361841	3.2:-78715429	MS.gene06538:CDS
TGAGTTGCTTAAGAAAATTA+AGG	0.374516	3.2:-78714850	MS.gene06538:intron
ACAGGCCAGCTGATGAATTT+GGG	0.393986	3.2:-78714485	MS.gene06538:CDS
TACTGCATGCCAGGGATGAC+TGG	0.411671	3.2:-78714876	MS.gene06538:CDS
GTGATGACCAAATTCACTTT+GGG	0.424646	3.2:+78714903	None:intergenic
ATCACAGACTACTGCATGCC+AGG	0.428078	3.2:-78714885	MS.gene06538:CDS
TTCTTCTTCAAAGTGGGTGA+TGG	0.456607	3.2:-78715436	MS.gene06538:CDS
CTGATTCTTCTTCTTCAAAG+TGG	0.467692	3.2:-78715443	MS.gene06538:CDS
TGATTCTTCTTCTTCAAAGT+GGG	0.471982	3.2:-78715442	MS.gene06538:CDS
ACCTTCCCAAATTCATCAGC+TGG	0.476038	3.2:+78714480	None:intergenic
TCAGATGACATGATCACAAC+AGG	0.480037	3.2:+78714692	None:intergenic
TCAGCAATCATCTGTGATGA+AGG	0.481432	3.2:-78714720	MS.gene06538:intron
TGTGATGACCAAATTCACTT+TGG	0.486577	3.2:+78714902	None:intergenic
GAACAGAACACTTTGGAGAA+TGG	0.490659	3.2:-78715070	MS.gene06538:CDS
CAGTGACAACTGCTGAAAAT+GGG	0.502968	3.2:-78715129	MS.gene06538:CDS
TTTACATCAGATTGTTTGAG+AGG	0.513388	3.2:+78714515	None:intergenic
TGAAAATGGGCTGAAGGCAT+TGG	0.515465	3.2:-78715116	MS.gene06538:CDS
AACTCATAGCCAGTCATCCC+TGG	0.516855	3.2:+78714867	None:intergenic
AAAAGCAGGTGTATGGAAGA+AGG	0.535716	3.2:-78714560	MS.gene06538:intron
AAAGCAGGTGTATGGAAGAA+GGG	0.542181	3.2:-78714559	MS.gene06538:intron
AACTGCTGAAAATGGGCTGA+AGG	0.568007	3.2:-78715122	MS.gene06538:CDS
CTCAAGAATTCCTCTTGCAA+AGG	0.589496	3.2:-78715342	MS.gene06538:intron
GCCAGCTGATGAATTTGGGA+AGG	0.598518	3.2:-78714481	MS.gene06538:CDS
AAGCAGGTGTATGGAAGAAG+GGG	0.612241	3.2:-78714558	MS.gene06538:intron
TCACAGACTACTGCATGCCA+GGG	0.612620	3.2:-78714884	MS.gene06538:CDS
CTTTGCAAGAGGAATTCTTG+AGG	0.621330	3.2:+78715343	None:intergenic
TCTGATGTAAAACAACTGAC+AGG	0.621647	3.2:-78714503	MS.gene06538:CDS
TCATCAACAGCAAGAACATG+AGG	0.625979	3.2:+78715399	None:intergenic
AAGTGGTGAACAGAACACTT+TGG	0.626039	3.2:-78715077	MS.gene06538:CDS
GAGTATTTGGGCTTGTCAAG+TGG	0.627865	3.2:-78715094	MS.gene06538:CDS
GGTGTACGTACCTTTGCAAG+AGG	0.636893	3.2:+78715332	None:intergenic
GCAATCATCTGTGATGAAGG+AGG	0.667691	3.2:-78714717	MS.gene06538:CDS
CATCTGAGAATATACCAACA+AGG	0.683899	3.2:-78714676	MS.gene06538:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAGAATATAACAGAAAA+TGG	+	chr3.2:78714624-78714643	None:intergenic	15.0%
!!	AGAAAAAGATACAAAAAAAA+TGG	+	chr3.2:78715303-78715322	None:intergenic	15.0%
!!	CTGAAAATAATATCCAATTT+GGG	+	chr3.2:78715180-78715199	None:intergenic	20.0%
!!	TGAAAATAATATCCAATTTG+GGG	+	chr3.2:78715179-78715198	None:intergenic	20.0%
!!!	TGATGTTTGATGTTTTTTTT+TGG	-	chr3.2:78714711-78714730	MS.gene06538:CDS	20.0%
!!!	TTTTTTTTTGGTCCATATTT+TGG	-	chr3.2:78714723-78714742	MS.gene06538:intron	20.0%
!	GCTGAAAATAATATCCAATT+TGG	+	chr3.2:78715181-78715200	None:intergenic	25.0%
!	TGAGTTGCTTAAGAAAATTA+AGG	-	chr3.2:78715066-78715085	MS.gene06538:intron	25.0%
!!!	GTGTTTTGTTAGTAACATTT+TGG	+	chr3.2:78714904-78714923	None:intergenic	25.0%
	AAATAATATCCAATTTGGGG+TGG	+	chr3.2:78715176-78715195	None:intergenic	30.0%
	CAAACAGAATCACCAAAATA+TGG	+	chr3.2:78714738-78714757	None:intergenic	30.0%
	GTGATTCTGTTTGATTGAAT+GGG	-	chr3.2:78714745-78714764	MS.gene06538:intron	30.0%
	TGATTCTTCTTCTTCAAAGT+GGG	-	chr3.2:78714474-78714493	MS.gene06538:CDS	30.0%
	TTTACATCAGATTGTTTGAG+AGG	+	chr3.2:78715404-78715423	None:intergenic	30.0%
!	AATTACTTGTTGATCCTTGT+TGG	+	chr3.2:78715257-78715276	None:intergenic	30.0%
!!!	GACTATTTCTGGTTTTTGTA+GGG	-	chr3.2:78714989-78715008	MS.gene06538:intron	30.0%
!!!	TGACTATTTCTGGTTTTTGT+AGG	-	chr3.2:78714988-78715007	MS.gene06538:intron	30.0%
	AAAATGGTTGAGTGAGAGAT+AGG	+	chr3.2:78714608-78714627	None:intergenic	35.0%
	CATCTGAGAATATACCAACA+AGG	-	chr3.2:78715240-78715259	MS.gene06538:intron	35.0%
	CTGATTCTTCTTCTTCAAAG+TGG	-	chr3.2:78714473-78714492	MS.gene06538:CDS	35.0%
	GGTGATTCTGTTTGATTGAA+TGG	-	chr3.2:78714744-78714763	MS.gene06538:intron	35.0%
	GTGATGACCAAATTCACTTT+GGG	+	chr3.2:78715016-78715035	None:intergenic	35.0%
	TCTGATGTAAAACAACTGAC+AGG	-	chr3.2:78715413-78715432	MS.gene06538:CDS	35.0%
	TGCTAACAAGTGACTATTTC+TGG	-	chr3.2:78714978-78714997	MS.gene06538:intron	35.0%
	TGTGATGACCAAATTCACTT+TGG	+	chr3.2:78715017-78715036	None:intergenic	35.0%
!!	TGATTGAATGGGCTTTTTGT+TGG	-	chr3.2:78714756-78714775	MS.gene06538:intron	35.0%
	AAAAGCAGGTGTATGGAAGA+AGG	-	chr3.2:78715356-78715375	MS.gene06538:CDS	40.0%
	AAAGCAGGTGTATGGAAGAA+GGG	-	chr3.2:78715357-78715376	MS.gene06538:CDS	40.0%
	AAGTGGTGAACAGAACACTT+TGG	-	chr3.2:78714839-78714858	MS.gene06538:intron	40.0%
	ACAGTGACAACTGCTGAAAA+TGG	-	chr3.2:78714786-78714805	MS.gene06538:intron	40.0%
	CAGCACAACAATGATTGAGT+AGG	+	chr3.2:78715335-78715354	None:intergenic	40.0%
	CAGTGACAACTGCTGAAAAT+GGG	-	chr3.2:78714787-78714806	MS.gene06538:intron	40.0%
	CATTGTTGTGCTGAAAAAGC+AGG	-	chr3.2:78715342-78715361	MS.gene06538:intron	40.0%
	CTCAAGAATTCCTCTTGCAA+AGG	-	chr3.2:78714574-78714593	MS.gene06538:intron	40.0%
	CTTTGCAAGAGGAATTCTTG+AGG	+	chr3.2:78714576-78714595	None:intergenic	40.0%
	TAACACTAACCACCCCAAAT+TGG	-	chr3.2:78715164-78715183	MS.gene06538:intron	40.0%
	TCAGATGACATGATCACAAC+AGG	+	chr3.2:78715227-78715246	None:intergenic	40.0%
	TCAGCAATCATCTGTGATGA+AGG	-	chr3.2:78715196-78715215	MS.gene06538:intron	40.0%
	TCATCAACAGCAAGAACATG+AGG	+	chr3.2:78714520-78714539	None:intergenic	40.0%
	TTCTTCTTCAAAGTGGGTGA+TGG	-	chr3.2:78714480-78714499	MS.gene06538:CDS	40.0%
!	GAACAGAACACTTTGGAGAA+TGG	-	chr3.2:78714846-78714865	MS.gene06538:intron	40.0%
!!	CTGAAGGCATTGGAGTATTT+GGG	-	chr3.2:78714810-78714829	MS.gene06538:intron	40.0%
!!	TCAAAGTGGGTGATGGAAAA+TGG	-	chr3.2:78714487-78714506	MS.gene06538:CDS	40.0%
	AAGCAGGTGTATGGAAGAAG+GGG	-	chr3.2:78715358-78715377	MS.gene06538:CDS	45.0%
	ACAGGCCAGCTGATGAATTT+GGG	-	chr3.2:78715431-78715450	MS.gene06538:CDS	45.0%
	ACCTTCCCAAATTCATCAGC+TGG	+	chr3.2:78715439-78715458	None:intergenic	45.0%
	GAGTATTTGGGCTTGTCAAG+TGG	-	chr3.2:78714822-78714841	MS.gene06538:intron	45.0%
	GCAATCATCTGTGATGAAGG+AGG	-	chr3.2:78715199-78715218	MS.gene06538:intron	45.0%
	GTAGGGACCCAAAGTGAATT+TGG	-	chr3.2:78715006-78715025	MS.gene06538:intron	45.0%
	GTGCTGAAAAAGCAGGTGTA+TGG	-	chr3.2:78715349-78715368	MS.gene06538:CDS	45.0%
!!	AACTGCTGAAAATGGGCTGA+AGG	-	chr3.2:78714794-78714813	MS.gene06538:intron	45.0%
!!	GCTGAAGGCATTGGAGTATT+TGG	-	chr3.2:78714809-78714828	MS.gene06538:intron	45.0%
!!	TGAAAATGGGCTGAAGGCAT+TGG	-	chr3.2:78714800-78714819	MS.gene06538:intron	45.0%
	AACTCATAGCCAGTCATCCC+TGG	+	chr3.2:78715052-78715071	None:intergenic	50.0%
	ATCACAGACTACTGCATGCC+AGG	-	chr3.2:78715031-78715050	MS.gene06538:intron	50.0%
	GACAGGCCAGCTGATGAATT+TGG	-	chr3.2:78715430-78715449	MS.gene06538:CDS	50.0%
	GCCAGCTGATGAATTTGGGA+AGG	-	chr3.2:78715435-78715454	MS.gene06538:CDS	50.0%
	GGTGTACGTACCTTTGCAAG+AGG	+	chr3.2:78714587-78714606	None:intergenic	50.0%
	TCACAGACTACTGCATGCCA+GGG	-	chr3.2:78715032-78715051	MS.gene06538:intron	50.0%
	TACTGCATGCCAGGGATGAC+TGG	-	chr3.2:78715040-78715059	MS.gene06538:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	78714466	78715472	78714466	ID=MS.gene06538
chr3.2	mRNA	78714466	78715472	78714466	ID=MS.gene06538.t1;Parent=MS.gene06538
chr3.2	exon	78715343	78715472	78715343	ID=MS.gene06538.t1.exon1;Parent=MS.gene06538.t1
chr3.2	CDS	78715343	78715472	78715343	ID=cds.MS.gene06538.t1;Parent=MS.gene06538.t1
chr3.2	exon	78715069	78715148	78715069	ID=MS.gene06538.t1.exon2;Parent=MS.gene06538.t1
chr3.2	CDS	78715069	78715148	78715069	ID=cds.MS.gene06538.t1;Parent=MS.gene06538.t1
chr3.2	exon	78714851	78714928	78714851	ID=MS.gene06538.t1.exon3;Parent=MS.gene06538.t1
chr3.2	CDS	78714851	78714928	78714851	ID=cds.MS.gene06538.t1;Parent=MS.gene06538.t1
chr3.2	exon	78714668	78714738	78714668	ID=MS.gene06538.t1.exon4;Parent=MS.gene06538.t1
chr3.2	CDS	78714668	78714738	78714668	ID=cds.MS.gene06538.t1;Parent=MS.gene06538.t1
chr3.2	exon	78714466	78714574	78714466	ID=MS.gene06538.t1.exon5;Parent=MS.gene06538.t1
chr3.2	CDS	78714466	78714574	78714466	ID=cds.MS.gene06538.t1;Parent=MS.gene06538.t1

Gene Sequence

>MS.gene06538

ATGGCTTCTGATTCTTCTTCTTCAAAGTGGGTGATGGAAAATGGTGACATTCCTCATGTTCTTGCTGTTGATGACAATATCATTGATCGCACACTTGTTGAGAAACTCCTCAAGAATTCCTCTTGCAAAGGTACGTACACCTATCTCTCACTCAACCATTTTCTGTTATATTCTTTTTCACTTTTTCAAACTAACTACTTTTACACTACACGTATCTCTTCTCTTCTCATTTACACACATGCTTTTGATGTTTGATGTTTTTTTTTGGTCCATATTTTGGTGATTCTGTTTGATTGAATGGGCTTTTTGTTGGATTCACTACAGTGACAACTGCTGAAAATGGGCTGAAGGCATTGGAGTATTTGGGCTTGTCAAGTGGTGAACAGAACACTTTGGAGAATGGAGTATGTTTTCTCTTTATGTGCATACATTTTACCAAAATGTTACTAACAAAACACACAATGACACTGAATTTGACATCAAATTTTGATGTTTAAAATTCATTTCTCACTTGCTAACAAGTGACTATTTCTGGTTTTTGTAGGGACCCAAAGTGAATTTGGTCATCACAGACTACTGCATGCCAGGGATGACTGGCTATGAGTTGCTTAAGAAAATTAAGGTACTTGCTGCAAAATCTTGTATATTACTATTTTTTTTTCACAATACAGTATTGCTGCATTATATATATGATATAGTAACACTAACCACCCCAAATTGGATATTATTTTCAGCAATCATCTGTGATGAAGGAGGTTCCTGTTGTGATCATGTCATCTGAGAATATACCAACAAGGATCAACAAGTAATTATTTGTCTCTAGCACTAACAATGCCATTTTTTTTGTATCTTTTTCTTTTGTTTAACCTACTCAATCATTGTTGTGCTGAAAAAGCAGGTGTATGGAAGAAGGGGCTCAGATGTTCATACAAAAGCCTCTCAAACAATCTGATGTAAAACAACTGACAGGCCAGCTGATGAATTTGGGAAGGTTGCAAAAGAATTGA

Protein sequence

>MS.gene06538.t1

MASDSSSSKWVMENGDIPHVLAVDDNIIDRTLVEKLLKNSSCKVTTAENGLKALEYLGLSSGEQNTLENGGPKVNLVITDYCMPGMTGYELLKKIKQSSVMKEVPVVIMSSENIPTRINKCMEEGAQMFIQKPLKQSDVKQLTGQLMNLGRLQKN