AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049408


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049408.t1 MTR_3g093860 92.357 157 9 2 1 155 1 156 2.73e-103 292
MS.gene049408.t1 MTR_1g049100 60.448 134 52 1 14 146 4 137 6.02e-55 171
MS.gene049408.t1 MTR_3g078613 60.769 130 50 1 19 148 24 152 9.77e-55 171
MS.gene049408.t1 MTR_3g078613 61.069 131 49 2 19 148 24 153 1.79e-53 168
MS.gene049408.t1 MTR_5g036480 60.606 132 47 2 19 146 17 147 4.98e-52 165
MS.gene049408.t1 MTR_8g038620 57.353 136 51 1 19 147 10 145 1.11e-51 162
MS.gene049408.t1 MTR_4g106590 60.305 131 50 2 19 148 27 156 1.64e-51 163
MS.gene049408.t1 MTR_7g490310 59.091 132 46 2 19 142 10 141 1.52e-50 162
MS.gene049408.t1 MTR_3g088630 53.191 141 48 2 19 142 23 162 6.96e-46 150
MS.gene049408.t1 MTR_3g015490 52.143 140 50 3 19 147 5 138 5.93e-43 140
MS.gene049408.t1 MTR_6g007460 40.659 182 54 3 19 147 12 192 6.62e-37 126
MS.gene049408.t1 MTR_2g050020 91.379 58 4 1 1 58 35 91 9.71e-31 107
MS.gene049408.t1 MTR_4g051330 56.863 51 22 0 97 147 77 127 3.71e-16 71.2
MS.gene049408.t1 MTR_4g061360 34.959 123 70 1 20 142 91 203 8.56e-16 73.9
MS.gene049408.t1 MTR_1g067110 34.959 123 70 1 20 142 87 199 6.41e-15 71.2
MS.gene049408.t1 MTR_1g067110 34.959 123 70 1 20 142 87 199 7.80e-15 70.9
MS.gene049408.t1 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.26e-14 68.2
MS.gene049408.t1 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.27e-14 68.2
MS.gene049408.t1 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.41e-14 68.2
MS.gene049408.t1 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.70e-14 68.2
MS.gene049408.t1 MTR_8g024260 30.894 123 75 1 20 142 38 150 3.14e-13 66.2
MS.gene049408.t1 MTR_7g118260 30.894 123 75 1 20 142 16 128 4.29e-13 65.9
MS.gene049408.t1 MTR_7g118260 30.894 123 75 1 20 142 16 128 5.02e-13 65.9
MS.gene049408.t1 MTR_8g079940 28.906 128 78 2 20 147 31 145 9.94e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049408.t1 AT3G56380 72.308 130 36 0 19 148 21 150 2.34e-64 194
MS.gene049408.t1 AT3G56380 72.519 131 35 1 19 148 21 151 4.69e-63 191
MS.gene049408.t1 AT2G40670 70.896 134 33 2 19 148 30 161 7.07e-63 191
MS.gene049408.t1 AT2G40670 71.111 135 32 3 19 148 30 162 1.45e-61 187
MS.gene049408.t1 AT1G10470 61.719 128 48 1 19 146 21 147 3.30e-53 169
MS.gene049408.t1 AT1G59940 58.647 133 54 1 14 146 29 160 4.81e-53 168
MS.gene049408.t1 AT1G10470 61.719 128 48 1 19 146 35 161 6.13e-53 168
MS.gene049408.t1 AT1G59940 58.647 133 54 1 14 146 121 252 7.17e-52 167
MS.gene049408.t1 AT3G48100 57.364 129 55 0 19 147 26 154 1.29e-51 162
MS.gene049408.t1 AT5G62920 58.955 134 54 1 19 152 26 158 3.10e-51 162
MS.gene049408.t1 AT3G57040 53.623 138 55 1 19 147 10 147 6.22e-51 162
MS.gene049408.t1 AT3G57040 53.623 138 55 1 19 147 10 147 6.22e-51 162
MS.gene049408.t1 AT2G41310 51.724 145 63 2 10 147 1 145 2.24e-50 161
MS.gene049408.t1 AT1G74890 59.091 132 53 1 19 150 19 149 5.77e-50 159
MS.gene049408.t1 AT1G19050 58.015 131 52 2 19 148 25 153 3.33e-49 157
MS.gene049408.t1 AT1G10470 50.000 74 33 3 75 146 14 85 3.10e-15 69.7
MS.gene049408.t1 AT5G24470 31.452 124 73 2 20 142 52 164 6.86e-14 68.2
MS.gene049408.t1 AT5G02810 31.707 123 74 1 20 142 80 192 2.07e-12 64.3
MS.gene049408.t1 AT5G60100 33.333 123 72 1 20 142 66 178 2.24e-12 63.9
MS.gene049408.t1 AT5G60100 33.333 123 72 1 20 142 66 178 2.24e-12 63.9
MS.gene049408.t1 AT5G60100 33.333 123 72 1 20 142 66 178 2.24e-12 63.9
MS.gene049408.t1 AT5G60100 33.333 123 72 1 20 142 66 178 2.25e-12 63.9
MS.gene049408.t1 AT5G60100 33.333 123 72 1 20 142 66 178 5.14e-12 62.8
MS.gene049408.t1 AT5G60100 33.333 123 72 1 20 142 66 178 5.14e-12 62.8
MS.gene049408.t1 AT5G60100 33.333 123 72 1 20 142 66 178 5.14e-12 62.8
MS.gene049408.t1 AT2G46790 28.226 124 79 1 19 142 38 151 1.85e-11 61.2
MS.gene049408.t1 AT2G46790 28.226 124 79 1 19 142 38 151 2.23e-11 61.2
MS.gene049408.t1 AT5G07210 30.081 123 72 3 20 142 18 126 3.21e-11 60.8

Find 39 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGAAGGCATTGGAGTATT+TGG 0.158019 3.1:-75092325 MS.gene049408:CDS
GACAGGCCAGCTGATGAATT+TGG 0.201243 3.1:-75091704 MS.gene049408:CDS
ACAGTGACAACTGCTGAAAA+TGG 0.248549 3.1:-75092348 MS.gene049408:intron
CTGAAGGCATTGGAGTATTT+GGG 0.249714 3.1:-75092324 MS.gene049408:CDS
GTAGGGACCCAAAGTGAATT+TGG 0.331645 3.1:-75092128 MS.gene049408:intron
AATTACTTGTTGATCCTTGT+TGG 0.359191 3.1:+75091880 None:intergenic
TCAAAGTGGGTGATGGAAAA+TGG 0.361841 3.1:-75092647 MS.gene049408:CDS
TGAGTTGCTTAAGAAAATTA+AGG 0.374516 3.1:-75092068 MS.gene049408:intron
ACAGGCCAGCTGATGAATTT+GGG 0.393986 3.1:-75091703 MS.gene049408:CDS
TACTGCATGCCAGGGATGAC+TGG 0.411671 3.1:-75092094 MS.gene049408:CDS
GTGATGACCAAATTCACTTT+GGG 0.424646 3.1:+75092121 None:intergenic
ATCACAGACTACTGCATGCC+AGG 0.428078 3.1:-75092103 MS.gene049408:CDS
TTCTTCTTCAAAGTGGGTGA+TGG 0.456607 3.1:-75092654 MS.gene049408:CDS
CTGATTCTTCTTCTTCAAAG+TGG 0.467692 3.1:-75092661 MS.gene049408:CDS
TGATTCTTCTTCTTCAAAGT+GGG 0.471982 3.1:-75092660 MS.gene049408:CDS
ACCTTCCCAAATTCATCAGC+TGG 0.476038 3.1:+75091698 None:intergenic
TCAGATGACATGATCACAAC+AGG 0.480037 3.1:+75091910 None:intergenic
TCAGCAATCATCTGTGATGA+AGG 0.481432 3.1:-75091938 MS.gene049408:intron
TGTGATGACCAAATTCACTT+TGG 0.486577 3.1:+75092120 None:intergenic
GAACAGAACACTTTGGAGAA+TGG 0.490659 3.1:-75092288 MS.gene049408:CDS
CAGTGACAACTGCTGAAAAT+GGG 0.502968 3.1:-75092347 MS.gene049408:CDS
TTTACATCAGATTGTTTGAG+AGG 0.513388 3.1:+75091733 None:intergenic
TGAAAATGGGCTGAAGGCAT+TGG 0.515465 3.1:-75092334 MS.gene049408:CDS
AACTCATAGCCAGTCATCCC+TGG 0.516855 3.1:+75092085 None:intergenic
AAAAGCAGGTGTATGGAAGA+AGG 0.535716 3.1:-75091778 MS.gene049408:intron
AAAGCAGGTGTATGGAAGAA+GGG 0.542181 3.1:-75091777 MS.gene049408:intron
AACTGCTGAAAATGGGCTGA+AGG 0.568007 3.1:-75092340 MS.gene049408:CDS
CTCAAGAATTCCTCTTGCAA+AGG 0.589496 3.1:-75092560 MS.gene049408:intron
GCCAGCTGATGAATTTGGGA+AGG 0.598518 3.1:-75091699 MS.gene049408:CDS
AAGCAGGTGTATGGAAGAAG+GGG 0.612241 3.1:-75091776 MS.gene049408:intron
TCACAGACTACTGCATGCCA+GGG 0.612620 3.1:-75092102 MS.gene049408:CDS
CTTTGCAAGAGGAATTCTTG+AGG 0.621330 3.1:+75092561 None:intergenic
TCTGATGTAAAACAACTGAC+AGG 0.621647 3.1:-75091721 MS.gene049408:CDS
TCATCAACAGCAAGAACATG+AGG 0.625979 3.1:+75092617 None:intergenic
AAGTGGTGAACAGAACACTT+TGG 0.626039 3.1:-75092295 MS.gene049408:CDS
GAGTATTTGGGCTTGTCAAG+TGG 0.627865 3.1:-75092312 MS.gene049408:CDS
GGTGTACGTACCTTTGCAAG+AGG 0.636893 3.1:+75092550 None:intergenic
GCAATCATCTGTGATGAAGG+AGG 0.667691 3.1:-75091935 MS.gene049408:CDS
CATCTGAGAATATACCAACA+AGG 0.683899 3.1:-75091894 MS.gene049408:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGAATATAACAGAAAA+TGG + chr3.1:75091842-75091861 None:intergenic 15.0%
!! AGAAAAAGATACAAAAAAAA+TGG + chr3.1:75092521-75092540 None:intergenic 15.0%
!! CTGAAAATAATATCCAATTT+GGG + chr3.1:75092398-75092417 None:intergenic 20.0%
!! TGAAAATAATATCCAATTTG+GGG + chr3.1:75092397-75092416 None:intergenic 20.0%
!!! TAAAAGTAGTTAGTTTGAAA+AGG + chr3.1:75091867-75091886 None:intergenic 20.0%
!!! TGATGTTTGATGTTTTTTTT+TGG - chr3.1:75091929-75091948 MS.gene049408:CDS 20.0%
!!! TTTTTTTTTGGTCCATATTT+TGG - chr3.1:75091941-75091960 MS.gene049408:intron 20.0%
! GCTGAAAATAATATCCAATT+TGG + chr3.1:75092399-75092418 None:intergenic 25.0%
! TGAGTTGCTTAAGAAAATTA+AGG - chr3.1:75092284-75092303 MS.gene049408:intron 25.0%
!!! GTGTTTTGTTAGTAACATTT+TGG + chr3.1:75092122-75092141 None:intergenic 25.0%
AAATAATATCCAATTTGGGG+TGG + chr3.1:75092394-75092413 None:intergenic 30.0%
CAAACAGAATCACCAAAATA+TGG + chr3.1:75091956-75091975 None:intergenic 30.0%
GTGATTCTGTTTGATTGAAT+GGG - chr3.1:75091963-75091982 MS.gene049408:intron 30.0%
TGATTCTTCTTCTTCAAAGT+GGG - chr3.1:75091692-75091711 MS.gene049408:CDS 30.0%
TTTACATCAGATTGTTTGAG+AGG + chr3.1:75092622-75092641 None:intergenic 30.0%
! AATTACTTGTTGATCCTTGT+TGG + chr3.1:75092475-75092494 None:intergenic 30.0%
!!! GACTATTTCTGGTTTTTGTA+GGG - chr3.1:75092207-75092226 MS.gene049408:intron 30.0%
!!! TGACTATTTCTGGTTTTTGT+AGG - chr3.1:75092206-75092225 MS.gene049408:intron 30.0%
AAAAATGGCATTGTTAGTGC+TGG + chr3.1:75092506-75092525 None:intergenic 35.0%
AAAATGGTTGAGTGAGAGAT+AGG + chr3.1:75091826-75091845 None:intergenic 35.0%
CATCTGAGAATATACCAACA+AGG - chr3.1:75092458-75092477 MS.gene049408:intron 35.0%
CTGATTCTTCTTCTTCAAAG+TGG - chr3.1:75091691-75091710 MS.gene049408:CDS 35.0%
GGTGATTCTGTTTGATTGAA+TGG - chr3.1:75091962-75091981 MS.gene049408:intron 35.0%
GTGATGACCAAATTCACTTT+GGG + chr3.1:75092234-75092253 None:intergenic 35.0%
TCTGATGTAAAACAACTGAC+AGG - chr3.1:75092631-75092650 MS.gene049408:CDS 35.0%
TGCTAACAAGTGACTATTTC+TGG - chr3.1:75092196-75092215 MS.gene049408:intron 35.0%
TGTGATGACCAAATTCACTT+TGG + chr3.1:75092235-75092254 None:intergenic 35.0%
!! TGATTGAATGGGCTTTTTGT+TGG - chr3.1:75091974-75091993 MS.gene049408:intron 35.0%
AAAAGCAGGTGTATGGAAGA+AGG - chr3.1:75092574-75092593 MS.gene049408:CDS 40.0%
AAAGCAGGTGTATGGAAGAA+GGG - chr3.1:75092575-75092594 MS.gene049408:CDS 40.0%
AAGTGGTGAACAGAACACTT+TGG - chr3.1:75092057-75092076 MS.gene049408:intron 40.0%
ACAGTGACAACTGCTGAAAA+TGG - chr3.1:75092004-75092023 MS.gene049408:intron 40.0%
CAGCACAACAATGATTGAGT+AGG + chr3.1:75092553-75092572 None:intergenic 40.0%
CAGTGACAACTGCTGAAAAT+GGG - chr3.1:75092005-75092024 MS.gene049408:intron 40.0%
CATTGTTGTGCTGAAAAAGC+AGG - chr3.1:75092560-75092579 MS.gene049408:intron 40.0%
CTCAAGAATTCCTCTTGCAA+AGG - chr3.1:75091792-75091811 MS.gene049408:intron 40.0%
CTTTGCAAGAGGAATTCTTG+AGG + chr3.1:75091794-75091813 None:intergenic 40.0%
TAACACTAACCACCCCAAAT+TGG - chr3.1:75092382-75092401 MS.gene049408:intron 40.0%
TCAGATGACATGATCACAAC+AGG + chr3.1:75092445-75092464 None:intergenic 40.0%
TCAGCAATCATCTGTGATGA+AGG - chr3.1:75092414-75092433 MS.gene049408:intron 40.0%
TCATCAACAGCAAGAACATG+AGG + chr3.1:75091738-75091757 None:intergenic 40.0%
TTCTTCTTCAAAGTGGGTGA+TGG - chr3.1:75091698-75091717 MS.gene049408:CDS 40.0%
! GAACAGAACACTTTGGAGAA+TGG - chr3.1:75092064-75092083 MS.gene049408:intron 40.0%
!! CTGAAGGCATTGGAGTATTT+GGG - chr3.1:75092028-75092047 MS.gene049408:intron 40.0%
!! TCAAAGTGGGTGATGGAAAA+TGG - chr3.1:75091705-75091724 MS.gene049408:CDS 40.0%
AAGCAGGTGTATGGAAGAAG+GGG - chr3.1:75092576-75092595 MS.gene049408:CDS 45.0%
ACAGGCCAGCTGATGAATTT+GGG - chr3.1:75092649-75092668 MS.gene049408:CDS 45.0%
ACCTTCCCAAATTCATCAGC+TGG + chr3.1:75092657-75092676 None:intergenic 45.0%
GAGTATTTGGGCTTGTCAAG+TGG - chr3.1:75092040-75092059 MS.gene049408:intron 45.0%
GCAATCATCTGTGATGAAGG+AGG - chr3.1:75092417-75092436 MS.gene049408:intron 45.0%
GTAGGGACCCAAAGTGAATT+TGG - chr3.1:75092224-75092243 MS.gene049408:intron 45.0%
GTGCTGAAAAAGCAGGTGTA+TGG - chr3.1:75092567-75092586 MS.gene049408:CDS 45.0%
!! AACTGCTGAAAATGGGCTGA+AGG - chr3.1:75092012-75092031 MS.gene049408:intron 45.0%
!! GCTGAAGGCATTGGAGTATT+TGG - chr3.1:75092027-75092046 MS.gene049408:intron 45.0%
!! TGAAAATGGGCTGAAGGCAT+TGG - chr3.1:75092018-75092037 MS.gene049408:intron 45.0%
AACTCATAGCCAGTCATCCC+TGG + chr3.1:75092270-75092289 None:intergenic 50.0%
ATCACAGACTACTGCATGCC+AGG - chr3.1:75092249-75092268 MS.gene049408:intron 50.0%
GACAGGCCAGCTGATGAATT+TGG - chr3.1:75092648-75092667 MS.gene049408:CDS 50.0%
GCCAGCTGATGAATTTGGGA+AGG - chr3.1:75092653-75092672 MS.gene049408:CDS 50.0%
GGTGTACGTACCTTTGCAAG+AGG + chr3.1:75091805-75091824 None:intergenic 50.0%
TCACAGACTACTGCATGCCA+GGG - chr3.1:75092250-75092269 MS.gene049408:intron 50.0%
TACTGCATGCCAGGGATGAC+TGG - chr3.1:75092258-75092277 MS.gene049408:intron 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 75091684 75092690 75091684 ID=MS.gene049408
chr3.1 mRNA 75091684 75092690 75091684 ID=MS.gene049408.t1;Parent=MS.gene049408
chr3.1 exon 75092561 75092690 75092561 ID=MS.gene049408.t1.exon1;Parent=MS.gene049408.t1
chr3.1 CDS 75092561 75092690 75092561 ID=cds.MS.gene049408.t1;Parent=MS.gene049408.t1
chr3.1 exon 75092287 75092366 75092287 ID=MS.gene049408.t1.exon2;Parent=MS.gene049408.t1
chr3.1 CDS 75092287 75092366 75092287 ID=cds.MS.gene049408.t1;Parent=MS.gene049408.t1
chr3.1 exon 75092069 75092146 75092069 ID=MS.gene049408.t1.exon3;Parent=MS.gene049408.t1
chr3.1 CDS 75092069 75092146 75092069 ID=cds.MS.gene049408.t1;Parent=MS.gene049408.t1
chr3.1 exon 75091886 75091956 75091886 ID=MS.gene049408.t1.exon4;Parent=MS.gene049408.t1
chr3.1 CDS 75091886 75091956 75091886 ID=cds.MS.gene049408.t1;Parent=MS.gene049408.t1
chr3.1 exon 75091684 75091792 75091684 ID=MS.gene049408.t1.exon5;Parent=MS.gene049408.t1
chr3.1 CDS 75091684 75091792 75091684 ID=cds.MS.gene049408.t1;Parent=MS.gene049408.t1
Gene Sequence

>MS.gene049408

ATGGCTTCTGATTCTTCTTCTTCAAAGTGGGTGATGGAAAATGGTGACATTCCTCATGTTCTTGCTGTTGATGACAATATCATTGATCGCACACTTGTTGAGAAACTCCTCAAGAATTCCTCTTGCAAAGGTACGTACACCTATCTCTCACTCAACCATTTTCTGTTATATTCTTTTTCACCTTTTCAAACTAACTACTTTTACACTACACGTATCTCTTCTCTTCTCATTTACACACATGCTTTTGATGTTTGATGTTTTTTTTTGGTCCATATTTTGGTGATTCTGTTTGATTGAATGGGCTTTTTGTTGGATACACTACAGTGACAACTGCTGAAAATGGGCTGAAGGCATTGGAGTATTTGGGCTTGTCAAGTGGTGAACAGAACACTTTGGAGAATGGAGTATGTTTTCTCTTTATGTGCATACATTTTACCAAAATGTTACTAACAAAACACACAATGACACTGAATTTGACATCAAATTTTGATGTTTAAAATTCATTTCTCACTTGCTAACAAGTGACTATTTCTGGTTTTTGTAGGGACCCAAAGTGAATTTGGTCATCACAGACTACTGCATGCCAGGGATGACTGGCTATGAGTTGCTTAAGAAAATTAAGGTACTTGCTGCAAAATCTTGTATATTACTATTTTTTTTTCACAATACAGTATTGCTGCATTATATATATGATATAGTAACACTAACCACCCCAAATTGGATATTATTTTCAGCAATCATCTGTGATGAAGGAGGTTCCTGTTGTGATCATGTCATCTGAGAATATACCAACAAGGATCAACAAGTAATTATTTGTCTCCAGCACTAACAATGCCATTTTTTTTGTATCTTTTTCTTTTGTTTAACCTACTCAATCATTGTTGTGCTGAAAAAGCAGGTGTATGGAAGAAGGGGCTCAGATGTTCATACAAAAGCCTCTCAAACAATCTGATGTAAAACAACTGACAGGCCAGCTGATGAATTTGGGAAGGTTGCAAAAGAATTGA

Protein sequence

>MS.gene049408.t1

MASDSSSSKWVMENGDIPHVLAVDDNIIDRTLVEKLLKNSSCKVTTAENGLKALEYLGLSSGEQNTLENGGPKVNLVITDYCMPGMTGYELLKKIKQSSVMKEVPVVIMSSENIPTRINKCMEEGAQMFIQKPLKQSDVKQLTGQLMNLGRLQKN