Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065408.t1 | XP_003624490.2 | 96.8 | 557 | 18 | 0 | 1 | 557 | 1 | 557 | 1.80E-297 | 1031.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065408.t1 | Q9LYU8 | 79.6 | 486 | 98 | 1 | 72 | 556 | 80 | 565 | 5.1e-208 | 725.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065408.t1 | G7KX84 | 96.8 | 557 | 18 | 0 | 1 | 557 | 1 | 557 | 1.3e-297 | 1031.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052115 | MS.gene065408 | 0.81398 | 1.95E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065408.t1 | MTR_7g083920 | 98.205 | 557 | 10 | 0 | 1 | 557 | 1 | 557 | 0.0 | 1122 |
| MS.gene065408.t1 | MTR_7g083920 | 98.144 | 431 | 8 | 0 | 127 | 557 | 20 | 450 | 0.0 | 866 |
| MS.gene065408.t1 | MTR_6g033260 | 81.139 | 509 | 89 | 2 | 51 | 553 | 47 | 554 | 0.0 | 825 |
| MS.gene065408.t1 | MTR_6g033260 | 80.822 | 511 | 89 | 3 | 51 | 553 | 47 | 556 | 0.0 | 820 |
| MS.gene065408.t1 | MTR_6g033260 | 83.120 | 468 | 73 | 1 | 51 | 512 | 47 | 514 | 0.0 | 786 |
| MS.gene065408.t1 | MTR_3g084500 | 28.216 | 482 | 300 | 12 | 81 | 534 | 79 | 542 | 7.59e-50 | 185 |
| MS.gene065408.t1 | MTR_8g083210 | 25.142 | 529 | 373 | 11 | 21 | 534 | 32 | 552 | 2.34e-48 | 181 |
| MS.gene065408.t1 | MTR_8g083210 | 26.501 | 483 | 330 | 12 | 71 | 534 | 69 | 545 | 5.40e-48 | 179 |
| MS.gene065408.t1 | MTR_3g084500 | 28.297 | 364 | 226 | 9 | 192 | 534 | 23 | 372 | 9.54e-38 | 148 |
| MS.gene065408.t1 | MTR_3g084500 | 28.882 | 322 | 197 | 8 | 234 | 534 | 12 | 322 | 2.24e-34 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065408.t1 | AT5G13280 | 81.070 | 486 | 91 | 1 | 72 | 556 | 80 | 565 | 0.0 | 789 |
| MS.gene065408.t1 | AT5G14060 | 80.388 | 464 | 89 | 1 | 74 | 535 | 78 | 541 | 0.0 | 754 |
| MS.gene065408.t1 | AT5G14060 | 80.388 | 464 | 89 | 1 | 74 | 535 | 78 | 541 | 0.0 | 754 |
| MS.gene065408.t1 | AT3G02020 | 74.950 | 499 | 118 | 2 | 46 | 541 | 55 | 549 | 0.0 | 754 |
| MS.gene065408.t1 | AT5G14060 | 81.167 | 377 | 71 | 0 | 74 | 450 | 78 | 454 | 0.0 | 632 |
| MS.gene065408.t1 | AT4G19710 | 27.140 | 479 | 307 | 11 | 81 | 534 | 91 | 552 | 7.94e-49 | 182 |
| MS.gene065408.t1 | AT4G19710 | 27.140 | 479 | 307 | 11 | 81 | 534 | 91 | 552 | 1.62e-48 | 181 |
| MS.gene065408.t1 | AT1G31230 | 27.801 | 482 | 300 | 12 | 81 | 534 | 86 | 547 | 1.79e-48 | 181 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGCGAGGGAAAGTGATTT+TGG | 0.193404 | 7.4:+23158515 | MS.gene065408:CDS |
| GCTGCATATCTTAATAAAAT+AGG | 0.208899 | 7.4:+23160553 | MS.gene065408:CDS |
| TACAGTGTTGTTATGAAATT+TGG | 0.211688 | 7.4:+23158569 | MS.gene065408:CDS |
| TTTGCTTGTGGGCATATATT+TGG | 0.219591 | 7.4:-23162982 | None:intergenic |
| TTGCAGGCCTTTCAAGTTCT+TGG | 0.229855 | 7.4:+23164591 | MS.gene065408:intron |
| CTTGCTCATGTCTCTTGTTT+TGG | 0.231285 | 7.4:-23163315 | None:intergenic |
| CTTCACAAATGCGGACATTT+TGG | 0.242277 | 7.4:+23160766 | MS.gene065408:CDS |
| CCTGCAATTCCAATTGTTAC+AGG | 0.246978 | 7.4:+23160842 | MS.gene065408:CDS |
| GTTTCCAATAAGAGAGATTA+TGG | 0.267691 | 7.4:-23164230 | None:intergenic |
| ATAAGGGTACCCGAAGCTTT+AGG | 0.282438 | 7.4:-23163290 | None:intergenic |
| AGATCCATAATCTCTCTTAT+TGG | 0.284492 | 7.4:+23164226 | MS.gene065408:CDS |
| CAAGATTACTTGGTTTCATT+TGG | 0.286947 | 7.4:+23160505 | MS.gene065408:CDS |
| ATCAGAACATCTAGAAGAAT+TGG | 0.321161 | 7.4:+23160432 | MS.gene065408:intron |
| AATGAATCCATACCTGAAAA+TGG | 0.329910 | 7.4:+23166136 | MS.gene065408:CDS |
| GATTTCTCAGGGTGCATCTA+AGG | 0.330625 | 7.4:+23164638 | MS.gene065408:CDS |
| ACTAGGACTGCGAGAGATTC+AGG | 0.335705 | 7.4:+23161082 | MS.gene065408:CDS |
| TTTAGGCATATCAATTGATA+TGG | 0.338260 | 7.4:+23163619 | MS.gene065408:CDS |
| TATCATTGTTCTTTCTGCTA+TGG | 0.342008 | 7.4:+23158667 | MS.gene065408:CDS |
| CCTGTAACAATTGGAATTGC+AGG | 0.347455 | 7.4:-23160842 | None:intergenic |
| GAGAAGTTGATATTCCTGTT+AGG | 0.349283 | 7.4:+23163249 | MS.gene065408:CDS |
| CGAAGAGGTTACATAGTGAT+TGG | 0.349771 | 7.4:+23160810 | MS.gene065408:CDS |
| TAAGGGTACCCGAAGCTTTA+GGG | 0.355181 | 7.4:-23163289 | None:intergenic |
| TCAGATGTCGTCACTGATAC+TGG | 0.365559 | 7.4:+23164255 | MS.gene065408:CDS |
| TTTCAAGTTCTTGGATCCCT+TGG | 0.372283 | 7.4:+23164600 | MS.gene065408:CDS |
| CCCGCATGCTTGGTGCGGTT+GGG | 0.373066 | 7.4:+23163484 | MS.gene065408:CDS |
| TTCCTACAACCCTAAAGCTT+CGG | 0.373093 | 7.4:+23163280 | MS.gene065408:CDS |
| CCAAGGAAGCCTGTAACAAT+TGG | 0.375737 | 7.4:-23160851 | None:intergenic |
| GATTTGACAGCTACAACAAT+TGG | 0.381611 | 7.4:+23161053 | MS.gene065408:CDS |
| CCAATTGTTACAGGCTTCCT+TGG | 0.388858 | 7.4:+23160851 | MS.gene065408:CDS |
| ATCATTGTTCTTTCTGCTAT+GGG | 0.389637 | 7.4:+23158668 | MS.gene065408:CDS |
| CAATGTTCAAATGATTTCTC+AGG | 0.391517 | 7.4:+23164626 | MS.gene065408:CDS |
| TGACTATGTTGTTGAAGAAC+TGG | 0.392763 | 7.4:+23164171 | MS.gene065408:CDS |
| TCCACGGACTCAAGATTACT+TGG | 0.393753 | 7.4:+23160495 | MS.gene065408:CDS |
| ACCCGCATGCTTGGTGCGGT+TGG | 0.395178 | 7.4:+23163483 | MS.gene065408:CDS |
| ACAATGATAGGCCTCTCTTC+AGG | 0.405492 | 7.4:-23158653 | None:intergenic |
| CTTTGTTACCGTGTCGAATC+GGG | 0.405814 | 7.4:+23158414 | MS.gene065408:CDS |
| TCTTTGTTACCGTGTCGAAT+CGG | 0.407129 | 7.4:+23158413 | MS.gene065408:CDS |
| TCCTACAACCCTAAAGCTTC+GGG | 0.420383 | 7.4:+23163281 | MS.gene065408:CDS |
| TCTTAATAAAATAGGTGTTA+AGG | 0.422364 | 7.4:+23160561 | MS.gene065408:CDS |
| AGAAGTTGATATTCCTGTTA+GGG | 0.422980 | 7.4:+23163250 | MS.gene065408:CDS |
| TCTGTTCGTACATTATCTTA+AGG | 0.426617 | 7.4:+23166160 | MS.gene065408:CDS |
| ATATAAAAGACTTGCATCTC+AGG | 0.437550 | 7.4:+23159695 | MS.gene065408:CDS |
| CAGTATGATGCATTCGAGAT+TGG | 0.438747 | 7.4:+23160725 | MS.gene065408:intron |
| CTCTTTCATCCTCACAGCAT+TGG | 0.439923 | 7.4:-23158606 | None:intergenic |
| AAATACCAGTAAAAGTTTGT+TGG | 0.449301 | 7.4:-23158699 | None:intergenic |
| ATAGCAGAAAGAACAATGAT+AGG | 0.458776 | 7.4:-23158665 | None:intergenic |
| TCAAATGTCAAATACGGAAC+AGG | 0.467011 | 7.4:-23163006 | None:intergenic |
| TGGGCATATATTTGGATCAC+AGG | 0.467793 | 7.4:-23162974 | None:intergenic |
| TGCGGTTGGGTTCCTTGCTA+AGG | 0.471236 | 7.4:+23163497 | MS.gene065408:CDS |
| TTCAGGACAGTGGAGGAACT+TGG | 0.473654 | 7.4:+23159835 | MS.gene065408:intron |
| TGTAACCTCTTCGCAACAGC+TGG | 0.480027 | 7.4:-23160800 | None:intergenic |
| AGCACCGAAGTATGCAAGTT+CGG | 0.480996 | 7.4:-23163037 | None:intergenic |
| ACCCGAAGCTTTAGGGTTGT+AGG | 0.482694 | 7.4:-23163282 | None:intergenic |
| GAAGAGGTTACATAGTGATT+GGG | 0.482865 | 7.4:+23160811 | MS.gene065408:CDS |
| GCCTCGTCAAATGTCAAATA+CGG | 0.487587 | 7.4:-23163012 | None:intergenic |
| CACTCGACACTTTCTTCACA+AGG | 0.494686 | 7.4:-23158463 | None:intergenic |
| ATTGTAAGCACCCGCATGCT+TGG | 0.497902 | 7.4:+23163474 | MS.gene065408:CDS |
| ATTTGACAGCTACAACAATT+GGG | 0.500909 | 7.4:+23161054 | MS.gene065408:CDS |
| CAAGGACAACATTGGGTAGA+GGG | 0.503097 | 7.4:+23161024 | MS.gene065408:CDS |
| ACTTGCATACTTCGGTGCTC+AGG | 0.503358 | 7.4:+23163041 | MS.gene065408:CDS |
| GTCTCATAGACTGTGGATGC+AGG | 0.505639 | 7.4:-23163220 | None:intergenic |
| TCATGTGCAAGGACAACATT+GGG | 0.510884 | 7.4:+23161017 | MS.gene065408:CDS |
| ACGAAGCAGAGCAGTCTGTC+AGG | 0.511744 | 7.4:+23166065 | MS.gene065408:CDS |
| CACAAAATCTTCTTTGAGTG+TGG | 0.514746 | 7.4:+23166094 | MS.gene065408:CDS |
| TAGCTCTCTGCTCCACAGCT+TGG | 0.525311 | 7.4:-23163684 | None:intergenic |
| ACAATCATGGCAACGCAAGT+TGG | 0.536528 | 7.4:+23158332 | None:intergenic |
| AGAATTGGAGCAACTTCTGA+AGG | 0.538970 | 7.4:+23160447 | MS.gene065408:CDS |
| GGAATATCAACTTCTCGAGC+AGG | 0.541819 | 7.4:-23163242 | None:intergenic |
| CTCGACACTTTCTTCACAAG+GGG | 0.544534 | 7.4:-23158461 | None:intergenic |
| CAGGTATGGAAAGATGTTGA+TGG | 0.545348 | 7.4:+23162943 | MS.gene065408:intron |
| GGTTAGTATTTCCTTGACGT+TGG | 0.549960 | 7.4:+23163655 | MS.gene065408:CDS |
| AGTGTTGTTATGAAATTTGG+TGG | 0.553672 | 7.4:+23158572 | MS.gene065408:CDS |
| CTTTCTTCACAAGGGGAACG+TGG | 0.553771 | 7.4:-23158454 | None:intergenic |
| TGTTACAGGCTTCCTTGGAA+AGG | 0.555752 | 7.4:+23160856 | MS.gene065408:CDS |
| CCCAACCGCACCAAGCATGC+GGG | 0.559440 | 7.4:-23163484 | None:intergenic |
| TTGCTTTGTATTCAGGACAG+TGG | 0.559449 | 7.4:+23159825 | MS.gene065408:intron |
| GCAAGGACAACATTGGGTAG+AGG | 0.560908 | 7.4:+23161023 | MS.gene065408:CDS |
| TTGGAGAGTGCATGTCCACT+AGG | 0.565887 | 7.4:+23160524 | MS.gene065408:CDS |
| CGTTGGATCCGTCCAAGCTG+TGG | 0.567000 | 7.4:+23163672 | MS.gene065408:CDS |
| ACAACAATTGGGAAAGCACT+AGG | 0.567209 | 7.4:+23161065 | MS.gene065408:CDS |
| GTTGTTGCAGCAACAATGCG+AGG | 0.567594 | 7.4:+23158501 | MS.gene065408:CDS |
| ACCCAACCGCACCAAGCATG+CGG | 0.572715 | 7.4:-23163485 | None:intergenic |
| AATGTTCAAATGATTTCTCA+GGG | 0.575084 | 7.4:+23164627 | MS.gene065408:CDS |
| AAGGGATCCAAGAACTTGAA+AGG | 0.580115 | 7.4:-23164598 | None:intergenic |
| TCTCTGCTCCACAGCTTGGA+CGG | 0.582859 | 7.4:-23163680 | None:intergenic |
| GCCGCCGAACTTGCATACTT+CGG | 0.588568 | 7.4:+23163033 | MS.gene065408:CDS |
| ACCAAGTAATCTTGAGTCCG+TGG | 0.593123 | 7.4:-23160496 | None:intergenic |
| ACTGAGCTCGTCGATACTAT+CGG | 0.597616 | 7.4:-23159671 | None:intergenic |
| AATTGGAGCAACTTCTGAAG+GGG | 0.602670 | 7.4:+23160449 | MS.gene065408:CDS |
| CATATCCAGCTGTTGCGAAG+AGG | 0.603370 | 7.4:+23160795 | MS.gene065408:CDS |
| ATCATGTGCAAGGACAACAT+TGG | 0.605197 | 7.4:+23161016 | MS.gene065408:CDS |
| GAATTGGAGCAACTTCTGAA+GGG | 0.606271 | 7.4:+23160448 | MS.gene065408:CDS |
| CTTTGTATTCAGGACAGTGG+AGG | 0.607780 | 7.4:+23159828 | MS.gene065408:intron |
| AAGGACAACATTGGGTAGAG+GGG | 0.613013 | 7.4:+23161025 | MS.gene065408:CDS |
| GTCGTCACTGATACTGGAGA+AGG | 0.617116 | 7.4:+23164261 | MS.gene065408:CDS |
| GTGTTGTTATGAAATTTGGT+GGG | 0.620539 | 7.4:+23158573 | MS.gene065408:CDS |
| CTTCTGTTGCCAATGCTGTG+AGG | 0.626415 | 7.4:+23158597 | MS.gene065408:CDS |
| GAAACGTAATGTTACCACGT+TGG | 0.628216 | 7.4:+23163449 | MS.gene065408:CDS |
| TTTCTTCACAAGGGGAACGT+GGG | 0.633560 | 7.4:-23158453 | None:intergenic |
| AACTGATGACTTCACAAATG+CGG | 0.637078 | 7.4:+23160757 | MS.gene065408:CDS |
| TCCGTATTTGACATTTGACG+AGG | 0.644289 | 7.4:+23163011 | MS.gene065408:CDS |
| AGGCTCGGAAATCATGTGCA+AGG | 0.645724 | 7.4:+23161006 | MS.gene065408:intron |
| AAGCACCCGCATGCTTGGTG+CGG | 0.646180 | 7.4:+23163479 | MS.gene065408:CDS |
| AGGACAACATTGGGTAGAGG+GGG | 0.646851 | 7.4:+23161026 | MS.gene065408:CDS |
| ACTCGACACTTTCTTCACAA+GGG | 0.646854 | 7.4:-23158462 | None:intergenic |
| CGAGCAGGTCTCATAGACTG+TGG | 0.649718 | 7.4:-23163227 | None:intergenic |
| TCATTTGAACATTGACACCA+AGG | 0.650137 | 7.4:-23164617 | None:intergenic |
| TGATGAAAGAACTGACTCCA+CGG | 0.656783 | 7.4:+23160479 | MS.gene065408:CDS |
| CATTTGAACATTGACACCAA+GGG | 0.662883 | 7.4:-23164616 | None:intergenic |
| TTGTTGCAGCAACAATGCGA+GGG | 0.676152 | 7.4:+23158502 | MS.gene065408:CDS |
| GCTGTGTACCCGATTCGACA+CGG | 0.676916 | 7.4:-23158422 | None:intergenic |
| ATATGCAGCAAAGATCCTAG+TGG | 0.678193 | 7.4:-23160539 | None:intergenic |
| CTTGGACGGATCCAACGTCA+AGG | 0.680723 | 7.4:-23163666 | None:intergenic |
| ACCGAAGTATGCAAGTTCGG+CGG | 0.683245 | 7.4:-23163034 | None:intergenic |
| CAAAACAAGAGACATGAGCA+AGG | 0.697597 | 7.4:+23163316 | MS.gene065408:CDS |
| TGATATGGTAGCTACAAGTG+AGG | 0.705689 | 7.4:+23163634 | MS.gene065408:CDS |
| GGTGCTTACAATATCCAACG+TGG | 0.806316 | 7.4:-23163463 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 23158338 | 23166180 | 23158338 | ID=MS.gene065408 |
| chr7.4 | mRNA | 23158338 | 23166180 | 23158338 | ID=MS.gene065408.t1;Parent=MS.gene065408 |
| chr7.4 | exon | 23158338 | 23158715 | 23158338 | ID=MS.gene065408.t1.exon1;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23158338 | 23158715 | 23158338 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23159634 | 23159716 | 23159634 | ID=MS.gene065408.t1.exon2;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23159634 | 23159716 | 23159634 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23159840 | 23159880 | 23159840 | ID=MS.gene065408.t1.exon3;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23159840 | 23159880 | 23159840 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23160437 | 23160591 | 23160437 | ID=MS.gene065408.t1.exon4;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23160437 | 23160591 | 23160437 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23160728 | 23160877 | 23160728 | ID=MS.gene065408.t1.exon5;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23160728 | 23160877 | 23160728 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23161008 | 23161103 | 23161008 | ID=MS.gene065408.t1.exon6;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23161008 | 23161103 | 23161008 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23162946 | 23163062 | 23162946 | ID=MS.gene065408.t1.exon7;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23162946 | 23163062 | 23162946 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23163218 | 23163337 | 23163218 | ID=MS.gene065408.t1.exon8;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23163218 | 23163337 | 23163218 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23163426 | 23163518 | 23163426 | ID=MS.gene065408.t1.exon9;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23163426 | 23163518 | 23163426 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23163599 | 23163715 | 23163599 | ID=MS.gene065408.t1.exon10;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23163599 | 23163715 | 23163599 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23164166 | 23164282 | 23164166 | ID=MS.gene065408.t1.exon11;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23164166 | 23164282 | 23164166 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23164597 | 23164659 | 23164597 | ID=MS.gene065408.t1.exon12;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23164597 | 23164659 | 23164597 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| chr7.4 | exon | 23166037 | 23166180 | 23166037 | ID=MS.gene065408.t1.exon13;Parent=MS.gene065408.t1 |
| chr7.4 | CDS | 23166037 | 23166180 | 23166037 | ID=cds.MS.gene065408.t1;Parent=MS.gene065408.t1 |
| Gene Sequence |
| Protein sequence |
