Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065610.t1 | GAU17025.1 | 89.9 | 99 | 9 | 1 | 1 | 98 | 1 | 99 | 4.90E-43 | 183.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065610.t1 | Q41015 | 48.8 | 123 | 52 | 4 | 1 | 118 | 1 | 117 | 2.7e-18 | 92.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065610.t1 | A0A2Z6LIM5 | 89.9 | 99 | 9 | 1 | 1 | 98 | 1 | 99 | 3.5e-43 | 183.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054277 | MS.gene065610 | 0.829594 | 4.72E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065610.t1 | MTR_6g045403 | 49.587 | 121 | 54 | 2 | 1 | 118 | 1 | 117 | 1.38e-30 | 108 |
| MS.gene065610.t1 | MTR_6g044780 | 48.361 | 122 | 55 | 2 | 1 | 118 | 1 | 118 | 4.10e-30 | 107 |
| MS.gene065610.t1 | MTR_6g045097 | 48.361 | 122 | 55 | 2 | 1 | 118 | 1 | 118 | 4.72e-30 | 107 |
| MS.gene065610.t1 | MTR_6g044810 | 46.721 | 122 | 57 | 2 | 1 | 118 | 1 | 118 | 9.13e-29 | 103 |
| MS.gene065610.t1 | MTR_6g045433 | 44.615 | 130 | 56 | 2 | 1 | 118 | 1 | 126 | 1.00e-27 | 101 |
| MS.gene065610.t1 | MTR_6g059760 | 47.107 | 121 | 53 | 4 | 1 | 118 | 1 | 113 | 1.21e-24 | 93.6 |
| MS.gene065610.t1 | MTR_6g059650 | 50.400 | 125 | 45 | 4 | 1 | 118 | 1 | 115 | 6.75e-22 | 86.3 |
| MS.gene065610.t1 | MTR_6g065460 | 45.455 | 121 | 57 | 4 | 1 | 118 | 1 | 115 | 3.47e-20 | 81.3 |
| MS.gene065610.t1 | MTR_6g059530 | 47.826 | 115 | 53 | 3 | 4 | 118 | 3 | 110 | 3.77e-20 | 81.6 |
| MS.gene065610.t1 | MTR_6g065460 | 45.455 | 121 | 57 | 4 | 1 | 118 | 1 | 115 | 6.52e-20 | 80.9 |
| MS.gene065610.t1 | MTR_6g017225 | 46.281 | 121 | 54 | 5 | 1 | 118 | 1 | 113 | 2.32e-19 | 82.0 |
| MS.gene065610.t1 | MTR_1g057110 | 52.174 | 69 | 30 | 1 | 1 | 66 | 1 | 69 | 1.32e-16 | 72.0 |
| MS.gene065610.t1 | MTR_6g059410 | 43.478 | 115 | 57 | 5 | 4 | 118 | 3 | 109 | 1.16e-15 | 69.7 |
| MS.gene065610.t1 | MTR_6g059730 | 41.667 | 120 | 62 | 3 | 1 | 117 | 1 | 115 | 2.89e-13 | 63.5 |
| MS.gene065610.t1 | MTR_6g059680 | 42.623 | 122 | 61 | 3 | 1 | 118 | 1 | 117 | 3.28e-13 | 63.5 |
| MS.gene065610.t1 | MTR_6g065570 | 47.826 | 69 | 28 | 3 | 13 | 77 | 9 | 73 | 7.89e-13 | 60.1 |
| MS.gene065610.t1 | MTR_6g066190 | 40.000 | 120 | 62 | 5 | 1 | 118 | 1 | 112 | 3.75e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGATGTCAAATACTTTATT+TGG | 0.154388 | 6.3:+63588171 | MS.gene065610:CDS |
| TCTACTATGGTAAATTTAAT+TGG | 0.206290 | 6.3:-63588311 | None:intergenic |
| ACTTGTTCTGTAAAGGCTAA+TGG | 0.267193 | 6.3:-63588119 | None:intergenic |
| CCACCAGTCGAAAAGGAATT+TGG | 0.267365 | 6.3:-63588194 | None:intergenic |
| TACTGGTAAGTGTGTAGATA+TGG | 0.306563 | 6.3:-63588354 | None:intergenic |
| GCTGTTGCTTATTTCTACTA+TGG | 0.344400 | 6.3:-63588324 | None:intergenic |
| GGTAAGTGATGTAGGCTTCA+TGG | 0.388637 | 6.3:-63588057 | None:intergenic |
| ATATCAAACTCAATAAATAC+TGG | 0.403900 | 6.3:-63588371 | None:intergenic |
| GTCCTTTACTTGTTCTGTAA+AGG | 0.421728 | 6.3:-63588126 | None:intergenic |
| AAATCTTGAAGTACAGTAGT+TGG | 0.464871 | 6.3:-63588266 | None:intergenic |
| GAAAGAGTGGTAAGTGATGT+AGG | 0.477308 | 6.3:-63588065 | None:intergenic |
| TGACACAAGGGAGTCGTGGC+AGG | 0.478078 | 6.3:+63588232 | MS.gene065610:CDS |
| GAACAAGTAAAGGACACAAA+TGG | 0.481025 | 6.3:+63588134 | MS.gene065610:CDS |
| CAGTCCACCACCAGTCGAAA+AGG | 0.495746 | 6.3:-63588201 | None:intergenic |
| TGTAAAGGCTAATGGAAAGT+AGG | 0.511536 | 6.3:-63588111 | None:intergenic |
| ACTGGTAAGTGTGTAGATAT+GGG | 0.531982 | 6.3:-63588353 | None:intergenic |
| AGCCTTTACAGAACAAGTAA+AGG | 0.533453 | 6.3:+63588124 | MS.gene065610:CDS |
| TAAATTTAATTGGCAAGCCA+TGG | 0.548633 | 6.3:-63588301 | None:intergenic |
| TTTCGACTGGTGGTGGACTG+AGG | 0.564095 | 6.3:+63588204 | MS.gene065610:CDS |
| ACTGGTGGTGGACTGAGGAT+TGG | 0.570318 | 6.3:+63588209 | MS.gene065610:CDS |
| GACTGAGGATTGGTGACACA+AGG | 0.587342 | 6.3:+63588219 | MS.gene065610:CDS |
| TAATGGAAAGTAGGTTGTGA+AGG | 0.599209 | 6.3:-63588102 | None:intergenic |
| TACAGTAGTTGGACATTCGT+CGG | 0.602808 | 6.3:-63588255 | None:intergenic |
| TTGGTGACACAAGGGAGTCG+TGG | 0.615771 | 6.3:+63588228 | MS.gene065610:CDS |
| AATGAGGAGCAAAGAAAGAG+TGG | 0.623665 | 6.3:-63588078 | None:intergenic |
| AGTAGGTTGTGAAGGCAATG+AGG | 0.647179 | 6.3:-63588094 | None:intergenic |
| ACTGAGGATTGGTGACACAA+GGG | 0.706089 | 6.3:+63588220 | MS.gene065610:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATCAAACTCAATAAATAC+TGG | - | chr6.3:63588374-63588393 | None:intergenic | 20.0% |
| !! | TCTACTATGGTAAATTTAAT+TGG | - | chr6.3:63588314-63588333 | None:intergenic | 20.0% |
| ! | ATTTGACATCAGCAAAAATA+GGG | - | chr6.3:63588163-63588182 | None:intergenic | 25.0% |
| ! | CTGATGTCAAATACTTTATT+TGG | + | chr6.3:63588171-63588190 | MS.gene065610:CDS | 25.0% |
| ! | TATTTGACATCAGCAAAAAT+AGG | - | chr6.3:63588164-63588183 | None:intergenic | 25.0% |
| AAATCTTGAAGTACAGTAGT+TGG | - | chr6.3:63588269-63588288 | None:intergenic | 30.0% | |
| TAAATTTAATTGGCAAGCCA+TGG | - | chr6.3:63588304-63588323 | None:intergenic | 30.0% | |
| ACTGGTAAGTGTGTAGATAT+GGG | - | chr6.3:63588356-63588375 | None:intergenic | 35.0% | |
| ACTTGTTCTGTAAAGGCTAA+TGG | - | chr6.3:63588122-63588141 | None:intergenic | 35.0% | |
| AGCCTTTACAGAACAAGTAA+AGG | + | chr6.3:63588124-63588143 | MS.gene065610:CDS | 35.0% | |
| GAACAAGTAAAGGACACAAA+TGG | + | chr6.3:63588134-63588153 | MS.gene065610:CDS | 35.0% | |
| GTCCTTTACTTGTTCTGTAA+AGG | - | chr6.3:63588129-63588148 | None:intergenic | 35.0% | |
| TAATGGAAAGTAGGTTGTGA+AGG | - | chr6.3:63588105-63588124 | None:intergenic | 35.0% | |
| TACTGGTAAGTGTGTAGATA+TGG | - | chr6.3:63588357-63588376 | None:intergenic | 35.0% | |
| TGTAAAGGCTAATGGAAAGT+AGG | - | chr6.3:63588114-63588133 | None:intergenic | 35.0% | |
| ! | GATTTTTCACAGAATGACCA+TGG | + | chr6.3:63588284-63588303 | MS.gene065610:CDS | 35.0% |
| !! | GCTGTTGCTTATTTCTACTA+TGG | - | chr6.3:63588327-63588346 | None:intergenic | 35.0% |
| AATGAGGAGCAAAGAAAGAG+TGG | - | chr6.3:63588081-63588100 | None:intergenic | 40.0% | |
| GAAAGAGTGGTAAGTGATGT+AGG | - | chr6.3:63588068-63588087 | None:intergenic | 40.0% | |
| TACAGTAGTTGGACATTCGT+CGG | - | chr6.3:63588258-63588277 | None:intergenic | 40.0% | |
| AGTAGGTTGTGAAGGCAATG+AGG | - | chr6.3:63588097-63588116 | None:intergenic | 45.0% | |
| CCACCAGTCGAAAAGGAATT+TGG | - | chr6.3:63588197-63588216 | None:intergenic | 45.0% | |
| ! | AATTCCTTTTCGACTGGTGG+TGG | + | chr6.3:63588197-63588216 | MS.gene065610:CDS | 45.0% |
| ! | ACTGAGGATTGGTGACACAA+GGG | + | chr6.3:63588220-63588239 | MS.gene065610:CDS | 45.0% |
| ! | CCAAATTCCTTTTCGACTGG+TGG | + | chr6.3:63588194-63588213 | MS.gene065610:CDS | 45.0% |
| ! | TGGCCAAATTCCTTTTCGAC+TGG | + | chr6.3:63588191-63588210 | MS.gene065610:CDS | 45.0% |
| ! | GACTGAGGATTGGTGACACA+AGG | + | chr6.3:63588219-63588238 | MS.gene065610:CDS | 50.0% |
| CAGTCCACCACCAGTCGAAA+AGG | - | chr6.3:63588204-63588223 | None:intergenic | 55.0% | |
| ! | TTGGTGACACAAGGGAGTCG+TGG | + | chr6.3:63588228-63588247 | MS.gene065610:CDS | 55.0% |
| !! | ACTGGTGGTGGACTGAGGAT+TGG | + | chr6.3:63588209-63588228 | MS.gene065610:CDS | 55.0% |
| !! | TTTCGACTGGTGGTGGACTG+AGG | + | chr6.3:63588204-63588223 | MS.gene065610:CDS | 55.0% |
| TGACACAAGGGAGTCGTGGC+AGG | + | chr6.3:63588232-63588251 | MS.gene065610:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 63588059 | 63588415 | 63588059 | ID=MS.gene065610 |
| chr6.3 | mRNA | 63588059 | 63588415 | 63588059 | ID=MS.gene065610.t1;Parent=MS.gene065610 |
| chr6.3 | exon | 63588059 | 63588415 | 63588059 | ID=MS.gene065610.t1.exon1;Parent=MS.gene065610.t1 |
| chr6.3 | CDS | 63588059 | 63588415 | 63588059 | ID=cds.MS.gene065610.t1;Parent=MS.gene065610.t1 |
| Gene Sequence |
| Protein sequence |
