Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065632.t1 | XP_024641890.1 | 97.7 | 175 | 4 | 0 | 1 | 175 | 4 | 178 | 2.20E-84 | 321.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065632.t1 | Q8VD57 | 35.5 | 166 | 89 | 3 | 9 | 170 | 6 | 157 | 1.2e-17 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065632.t1 | I3T2F0 | 97.7 | 175 | 4 | 0 | 1 | 175 | 4 | 178 | 1.6e-84 | 321.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051247 | MS.gene065632 | 0.819425 | 1.17E-52 | -1.69E-46 |
| MS.gene051594 | MS.gene065632 | 0.804976 | 1.67E-49 | -1.69E-46 |
| MS.gene051957 | MS.gene065632 | 0.816877 | 4.41E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065632.t1 | MTR_1g026920 | 55.063 | 158 | 64 | 1 | 18 | 175 | 12 | 162 | 3.58e-57 | 177 |
| MS.gene065632.t1 | MTR_6g064950 | 94.118 | 85 | 5 | 0 | 91 | 175 | 39 | 123 | 1.08e-53 | 167 |
| MS.gene065632.t1 | MTR_1g026920 | 52.174 | 115 | 48 | 1 | 18 | 132 | 12 | 119 | 3.36e-35 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065632.t1 | AT5G23550 | 75.429 | 175 | 40 | 2 | 1 | 175 | 4 | 175 | 5.20e-96 | 276 |
| MS.gene065632.t1 | AT5G24170 | 58.042 | 143 | 59 | 1 | 33 | 174 | 20 | 162 | 5.65e-54 | 169 |
| MS.gene065632.t1 | AT5G24170 | 55.000 | 100 | 44 | 1 | 33 | 131 | 20 | 119 | 1.57e-32 | 113 |
| MS.gene065632.t1 | AT4G26550 | 33.333 | 114 | 71 | 1 | 53 | 161 | 93 | 206 | 9.12e-11 | 58.9 |
Find 36 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAAAGCTCCTTCGCTTTCA+TGG | 0.196461 | 6.3:+64009348 | MS.gene065632:CDS |
| CATCATCGATACTTGCCTTT+TGG | 0.243479 | 6.3:-64015183 | None:intergenic |
| TGGAGTTTGGAGCACTAATT+TGG | 0.256506 | 6.3:+64015413 | MS.gene065632:CDS |
| TTCCTCAAACCAATCAAGTT+TGG | 0.259395 | 6.3:+64011865 | MS.gene065632:CDS |
| ATTTGTGACCAGAGATTATA+TGG | 0.287623 | 6.3:+64011577 | MS.gene065632:intron |
| CTTGCGATTATCTTGGAGTT+TGG | 0.300730 | 6.3:+64015400 | MS.gene065632:CDS |
| TCCTCAAACCAATCAAGTTT+GGG | 0.333791 | 6.3:+64011866 | MS.gene065632:CDS |
| TATCATACTTGCGATGTATA+TGG | 0.341256 | 6.3:-64015234 | None:intergenic |
| GACATTTCTTGCGATTATCT+TGG | 0.352149 | 6.3:+64015393 | MS.gene065632:CDS |
| AATGTTATCCCAAACTTGAT+TGG | 0.371483 | 6.3:-64011874 | None:intergenic |
| TTTGCCATTTGCTTTGCTGC+TGG | 0.373336 | 6.3:+64011601 | MS.gene065632:CDS |
| TCTTGGAAATTTGCTTTCAC+TGG | 0.383477 | 6.3:+64011900 | MS.gene065632:CDS |
| GTCCATGGTTTCAAGGATCA+TGG | 0.431757 | 6.3:+64016431 | MS.gene065632:CDS |
| TTTGGGATAACATTCACTCT+TGG | 0.432757 | 6.3:+64011883 | MS.gene065632:CDS |
| TTGCGAGGTCCATGGTTTCA+AGG | 0.447760 | 6.3:+64016424 | MS.gene065632:CDS |
| TTTCGAGAAAGTGAAAATAA+TGG | 0.449621 | 6.3:+64009276 | MS.gene065632:CDS |
| TCCCAAACTTGATTGGTTTG+AGG | 0.451750 | 6.3:-64011867 | None:intergenic |
| GAAACCATGGACCTCGCAAA+AGG | 0.453828 | 6.3:-64016420 | None:intergenic |
| CTTGGAAATTTGCTTTCACT+GGG | 0.482081 | 6.3:+64011901 | MS.gene065632:CDS |
| GCTGTTGCATAAATACGAAC+AGG | 0.487558 | 6.3:-64015212 | None:intergenic |
| AGAGGCACAAGCTGCTGCAT+TGG | 0.489920 | 6.3:+64009321 | MS.gene065632:CDS |
| AGTGAAAATAATGGTGGGAA+TGG | 0.497361 | 6.3:+64009285 | MS.gene065632:CDS |
| CAAACTTACCAACAGTGTAA+AGG | 0.506500 | 6.3:-64011629 | None:intergenic |
| TACTCCAGCAGCAAAGCAAA+TGG | 0.511667 | 6.3:-64011605 | None:intergenic |
| GAAATTTGCTTTCACTGGGA+AGG | 0.539615 | 6.3:+64011905 | MS.gene065632:CDS |
| CATTCCTCATAGGTCCCAAA+AGG | 0.540053 | 6.3:+64015168 | MS.gene065632:CDS |
| AAACCATGGACCTCGCAAAA+GGG | 0.541259 | 6.3:-64016419 | None:intergenic |
| CGAGAAAGTGAAAATAATGG+TGG | 0.545878 | 6.3:+64009279 | MS.gene065632:CDS |
| GGCAGCACAGCATTCCTCAT+AGG | 0.551773 | 6.3:+64015158 | MS.gene065632:intron |
| CTGTACTTTGTCTAGAACAC+AGG | 0.566493 | 6.3:+64009387 | MS.gene065632:CDS |
| TGGAGTAACCTTTACACTGT+TGG | 0.575811 | 6.3:+64011621 | MS.gene065632:CDS |
| AACCATGATCCTTGAAACCA+TGG | 0.581792 | 6.3:-64016433 | None:intergenic |
| TGGTTTGAGGAAAACAAGCA+TGG | 0.594589 | 6.3:-64011854 | None:intergenic |
| GAGAAAGTGAAAATAATGGT+GGG | 0.605154 | 6.3:+64009280 | MS.gene065632:CDS |
| GAAATCATCCATGAAAGCGA+AGG | 0.620762 | 6.3:-64009356 | None:intergenic |
| AATGGAAGTCGAAGACGAAG+AGG | 0.644986 | 6.3:+64009303 | MS.gene065632:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 64009265 | 64016479 | 64009265 | ID=MS.gene065632 |
| chr6.3 | mRNA | 64009265 | 64016479 | 64009265 | ID=MS.gene065632.t1;Parent=MS.gene065632 |
| chr6.3 | exon | 64009265 | 64009408 | 64009265 | ID=MS.gene065632.t1.exon1;Parent=MS.gene065632.t1 |
| chr6.3 | CDS | 64009265 | 64009408 | 64009265 | ID=cds.MS.gene065632.t1;Parent=MS.gene065632.t1 |
| chr6.3 | exon | 64011589 | 64011642 | 64011589 | ID=MS.gene065632.t1.exon2;Parent=MS.gene065632.t1 |
| chr6.3 | CDS | 64011589 | 64011642 | 64011589 | ID=cds.MS.gene065632.t1;Parent=MS.gene065632.t1 |
| chr6.3 | exon | 64011853 | 64011926 | 64011853 | ID=MS.gene065632.t1.exon3;Parent=MS.gene065632.t1 |
| chr6.3 | CDS | 64011853 | 64011926 | 64011853 | ID=cds.MS.gene065632.t1;Parent=MS.gene065632.t1 |
| chr6.3 | exon | 64015163 | 64015280 | 64015163 | ID=MS.gene065632.t1.exon4;Parent=MS.gene065632.t1 |
| chr6.3 | CDS | 64015163 | 64015280 | 64015163 | ID=cds.MS.gene065632.t1;Parent=MS.gene065632.t1 |
| chr6.3 | exon | 64015376 | 64015434 | 64015376 | ID=MS.gene065632.t1.exon5;Parent=MS.gene065632.t1 |
| chr6.3 | CDS | 64015376 | 64015434 | 64015376 | ID=cds.MS.gene065632.t1;Parent=MS.gene065632.t1 |
| chr6.3 | exon | 64016401 | 64016479 | 64016401 | ID=MS.gene065632.t1.exon6;Parent=MS.gene065632.t1 |
| chr6.3 | CDS | 64016401 | 64016479 | 64016401 | ID=cds.MS.gene065632.t1;Parent=MS.gene065632.t1 |
| Gene Sequence |
| Protein sequence |
