Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065777.t1 | PNX61557.1 | 57.5 | 80 | 21 | 2 | 4 | 83 | 34 | 100 | 6.30E-13 | 83.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065777.t1 | A2RVP7 | 76.9 | 26 | 6 | 0 | 57 | 82 | 147 | 172 | 6.4e-06 | 51.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065777.t1 | A0A2K3K5J7 | 57.5 | 80 | 21 | 2 | 4 | 83 | 34 | 100 | 4.5e-13 | 83.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055808 | MS.gene065777 | 0.806457 | 8.15E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene065777 | 0.833189 | 6.15E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065777.t1 | MTR_4g077950 | 70.455 | 44 | 8 | 1 | 40 | 83 | 132 | 170 | 1.73e-13 | 62.0 |
| MS.gene065777.t1 | MTR_2g070380 | 70.455 | 44 | 8 | 1 | 40 | 83 | 132 | 170 | 1.75e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCGTCATAGAGAAGTTTC+TGG | 0.329705 | 4.4:-32572786 | MS.gene065777:CDS |
| AGAAGAAAGTTGTGCTTTGT+AGG | 0.359959 | 4.4:-32573036 | MS.gene065777:CDS |
| TGGTCCACAAGCTGCATGTA+TGG | 0.406432 | 4.4:-32572834 | MS.gene065777:CDS |
| TCATCCAGGGCACTTCTAGA+AGG | 0.415331 | 4.4:-32573341 | MS.gene065777:intron |
| TCTATTGAGTGTGGTACAAA+GGG | 0.453200 | 4.4:-32573397 | MS.gene065777:CDS |
| GCACAAAGTGATGTTCATCC+AGG | 0.457102 | 4.4:-32573355 | MS.gene065777:CDS |
| GTTTCTGGAGAGGCATCAAT+AGG | 0.471586 | 4.4:-32572771 | None:intergenic |
| CTCTATTGAGTGTGGTACAA+AGG | 0.510090 | 4.4:-32573398 | MS.gene065777:CDS |
| AACGCAAATTGATGTAAAAG+AGG | 0.515061 | 4.4:-32573001 | MS.gene065777:CDS |
| CTCTAATTCTCTATTGAGTG+TGG | 0.523105 | 4.4:-32573406 | MS.gene065777:CDS |
| GCTGCATGTATGGGATGTGC+TGG | 0.545754 | 4.4:-32572824 | MS.gene065777:CDS |
| GAAACCTTCTAGAAGTGCCC+TGG | 0.550153 | 4.4:+32573337 | None:intergenic |
| CATCCCATACATGCAGCTTG+TGG | 0.593009 | 4.4:+32572830 | None:intergenic |
| CACAAAGTGATGTTCATCCA+GGG | 0.608884 | 4.4:-32573354 | MS.gene065777:CDS |
| CTCTATGACGACAGAGAATG+CGG | 0.617557 | 4.4:+32572796 | None:intergenic |
| GGTCCACAAGCTGCATGTAT+GGG | 0.631485 | 4.4:-32572833 | MS.gene065777:CDS |
| TCATAGAGAAGTTTCTGGAG+AGG | 0.660052 | 4.4:-32572781 | MS.gene065777:CDS |
| TATTGAGTGTGGTACAAAGG+GGG | 0.683621 | 4.4:-32573395 | MS.gene065777:CDS |
| CTATTGAGTGTGGTACAAAG+GGG | 0.695389 | 4.4:-32573396 | MS.gene065777:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAGAAAACAACTTTGAAA+AGG | + | chr4.4:32572956-32572975 | None:intergenic | 20.0% |
| !!! | ATAGAAAACAACTTTGAAAA+GGG | + | chr4.4:32572955-32572974 | None:intergenic | 20.0% |
| !! | CAATTGTGTATTCTGATTTT+TGG | + | chr4.4:32573008-32573027 | None:intergenic | 25.0% |
| !! | CTAACAATAACTTCTGTTTT+GGG | + | chr4.4:32572917-32572936 | None:intergenic | 25.0% |
| !! | TCTAACAATAACTTCTGTTT+TGG | + | chr4.4:32572918-32572937 | None:intergenic | 25.0% |
| AACGCAAATTGATGTAAAAG+AGG | - | chr4.4:32573188-32573207 | MS.gene065777:intron | 30.0% | |
| ATTGAGCACTAAATCCATAT+TGG | + | chr4.4:32573070-32573089 | None:intergenic | 30.0% | |
| TTGAGCACTAAATCCATATT+GGG | + | chr4.4:32573069-32573088 | None:intergenic | 30.0% | |
| ACAACTTTCTTCTGCAATGA+GGG | + | chr4.4:32573146-32573165 | None:intergenic | 35.0% | |
| AGAAGAAAGTTGTGCTTTGT+AGG | - | chr4.4:32573153-32573172 | MS.gene065777:intron | 35.0% | |
| CTCTAATTCTCTATTGAGTG+TGG | - | chr4.4:32572783-32572802 | MS.gene065777:CDS | 35.0% | |
| GGAGAAAAGAAACAGTAATC+AGG | - | chr4.4:32573209-32573228 | MS.gene065777:intron | 35.0% | |
| TACTGTTTCCAATGTTTGTG+TGG | - | chr4.4:32573325-32573344 | MS.gene065777:intron | 35.0% | |
| TCTATTGAGTGTGGTACAAA+GGG | - | chr4.4:32572792-32572811 | MS.gene065777:CDS | 35.0% | |
| ! | CTAGAAGGTTTCTGTCTTTT+TGG | - | chr4.4:32572863-32572882 | MS.gene065777:intron | 35.0% |
| ! | TCGTTTTCCAGTTCATTATC+TGG | + | chr4.4:32573235-32573254 | None:intergenic | 35.0% |
| AACCGCAAGTTCTCAAATCA+TGG | - | chr4.4:32573029-32573048 | MS.gene065777:CDS | 40.0% | |
| AATCAGGCCAGATAATGAAC+TGG | - | chr4.4:32573225-32573244 | MS.gene065777:intron | 40.0% | |
| AATGTTTGTGTGGTTGTAGG+TGG | - | chr4.4:32573335-32573354 | MS.gene065777:intron | 40.0% | |
| ACCGCAAGTTCTCAAATCAT+GGG | - | chr4.4:32573030-32573049 | MS.gene065777:CDS | 40.0% | |
| ACCTACAACCACACAAACAT+TGG | + | chr4.4:32573336-32573355 | None:intergenic | 40.0% | |
| CACAAAGTGATGTTCATCCA+GGG | - | chr4.4:32572835-32572854 | MS.gene065777:CDS | 40.0% | |
| CACAACTTTCTTCTGCAATG+AGG | + | chr4.4:32573147-32573166 | None:intergenic | 40.0% | |
| CTATTGAGTGTGGTACAAAG+GGG | - | chr4.4:32572793-32572812 | MS.gene065777:CDS | 40.0% | |
| CTCTATTGAGTGTGGTACAA+AGG | - | chr4.4:32572791-32572810 | MS.gene065777:CDS | 40.0% | |
| TATTGAGTGTGGTACAAAGG+GGG | - | chr4.4:32572794-32572813 | MS.gene065777:CDS | 40.0% | |
| TCCAATGTTTGTGTGGTTGT+AGG | - | chr4.4:32573332-32573351 | MS.gene065777:intron | 40.0% | |
| TGTCGTCATAGAGAAGTTTC+TGG | - | chr4.4:32573403-32573422 | MS.gene065777:CDS | 40.0% | |
| ! | GAAGGTTTCTGTCTTTTTGG+TGG | - | chr4.4:32572866-32572885 | MS.gene065777:intron | 40.0% |
| ! | TCATAGAGAAGTTTCTGGAG+AGG | - | chr4.4:32573408-32573427 | MS.gene065777:CDS | 40.0% |
| CCCCATGATTTGAGAACTTG+CGG | + | chr4.4:32573034-32573053 | None:intergenic | 45.0% | |
| CCGCAAGTTCTCAAATCATG+GGG | - | chr4.4:32573031-32573050 | MS.gene065777:CDS | 45.0% | |
| CTCTATGACGACAGAGAATG+CGG | + | chr4.4:32573396-32573415 | None:intergenic | 45.0% | |
| GCACAAAGTGATGTTCATCC+AGG | - | chr4.4:32572834-32572853 | MS.gene065777:CDS | 45.0% | |
| ! | AAATCATGGGGTTCTGACAG+TGG | - | chr4.4:32573043-32573062 | MS.gene065777:intron | 45.0% |
| CATCCCATACATGCAGCTTG+TGG | + | chr4.4:32573362-32573381 | None:intergenic | 50.0% | |
| GGTCCACAAGCTGCATGTAT+GGG | - | chr4.4:32573356-32573375 | MS.gene065777:CDS | 50.0% | |
| GTTCTGACAGTGGCCCAATA+TGG | - | chr4.4:32573053-32573072 | MS.gene065777:intron | 50.0% | |
| TGGTCCACAAGCTGCATGTA+TGG | - | chr4.4:32573355-32573374 | MS.gene065777:CDS | 50.0% | |
| ! | TCATCCAGGGCACTTCTAGA+AGG | - | chr4.4:32572848-32572867 | MS.gene065777:intron | 50.0% |
| !! | GAAACCTTCTAGAAGTGCCC+TGG | + | chr4.4:32572855-32572874 | None:intergenic | 50.0% |
| AGTGTGGTACAAAGGGGGTC+TGG | - | chr4.4:32572799-32572818 | MS.gene065777:CDS | 55.0% | |
| CAAAGGGGGTCTGGTGTGAA+TGG | - | chr4.4:32572808-32572827 | MS.gene065777:CDS | 55.0% | |
| GCTGCATGTATGGGATGTGC+TGG | - | chr4.4:32573365-32573384 | MS.gene065777:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 32572772 | 32573439 | 32572772 | ID=MS.gene065777 |
| chr4.4 | mRNA | 32572772 | 32573439 | 32572772 | ID=MS.gene065777.t1;Parent=MS.gene065777 |
| chr4.4 | exon | 32573342 | 32573439 | 32573342 | ID=MS.gene065777.t1.exon1;Parent=MS.gene065777.t1 |
| chr4.4 | CDS | 32573342 | 32573439 | 32573342 | ID=cds.MS.gene065777.t1;Parent=MS.gene065777.t1 |
| chr4.4 | exon | 32572989 | 32573056 | 32572989 | ID=MS.gene065777.t1.exon2;Parent=MS.gene065777.t1 |
| chr4.4 | CDS | 32572989 | 32573056 | 32572989 | ID=cds.MS.gene065777.t1;Parent=MS.gene065777.t1 |
| chr4.4 | exon | 32572772 | 32572857 | 32572772 | ID=MS.gene065777.t1.exon3;Parent=MS.gene065777.t1 |
| chr4.4 | CDS | 32572772 | 32572857 | 32572772 | ID=cds.MS.gene065777.t1;Parent=MS.gene065777.t1 |
| Gene Sequence |
| Protein sequence |
