Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065953.t1 | XP_013443646.1 | 95.9 | 170 | 5 | 2 | 1 | 169 | 62 | 230 | 1.30E-76 | 295.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065953.t1 | Q38847 | 46.7 | 169 | 73 | 2 | 1 | 169 | 62 | 213 | 9.3e-28 | 124.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065953.t1 | A0A072TKE2 | 95.9 | 170 | 5 | 2 | 1 | 169 | 62 | 230 | 9.1e-77 | 295.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055311 | MS.gene065953 | 0.823993 | 1.03E-53 | -1.69E-46 |
| MS.gene055313 | MS.gene065953 | 0.802084 | 6.63E-49 | -1.69E-46 |
| MS.gene058646 | MS.gene065953 | 0.804925 | 1.71E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGACACCACATTGCAATT+AGG | 0.257094 | 4.4:+63369433 | MS.gene065953:CDS |
| CAGACACCACATTGCAATTA+GGG | 0.281450 | 4.4:+63369434 | MS.gene065953:CDS |
| AAGAACTCCTTTGTAGATAA+TGG | 0.328915 | 4.4:+63369361 | MS.gene065953:CDS |
| TACTTCCGAAGGAAGATTCT+AGG | 0.353361 | 4.4:+63367899 | MS.gene065953:CDS |
| CTTATCTGGATTGGGAATAA+AGG | 0.357344 | 4.4:+63368100 | MS.gene065953:CDS |
| GGAATTACTCATGGAACAAC+TGG | 0.394806 | 4.4:+63368460 | MS.gene065953:CDS |
| CTGTTGGGAAAGGACTTATC+TGG | 0.395296 | 4.4:+63368086 | MS.gene065953:CDS |
| GGGAAAGGACTTATCTGGAT+TGG | 0.415856 | 4.4:+63368091 | MS.gene065953:CDS |
| GGCATAACATGTTCTGTCAT+TGG | 0.418254 | 4.4:-63369307 | None:intergenic |
| GGAAAGGACTTATCTGGATT+GGG | 0.419728 | 4.4:+63368092 | MS.gene065953:CDS |
| GAATGAAACTTTGCGAAGAC+AGG | 0.464677 | 4.4:+63369158 | MS.gene065953:CDS |
| CCAGGAGCAGAAGGCTATGC+TGG | 0.465487 | 4.4:+63369134 | MS.gene065953:intron |
| ACTGGAGCAGTCTCGAATAC+AGG | 0.492606 | 4.4:+63368478 | MS.gene065953:CDS |
| TGAGCAGTTTCTAGTCAAGC+TGG | 0.492674 | 4.4:-63369395 | None:intergenic |
| TGTGACTATAGACGACTGTT+GGG | 0.503182 | 4.4:+63368071 | MS.gene065953:intron |
| AAGGACCTTCCTCTCCTTCA+CGG | 0.505318 | 4.4:-63368165 | None:intergenic |
| TTGTGACTATAGACGACTGT+TGG | 0.511744 | 4.4:+63368070 | MS.gene065953:intron |
| ACCATCCTAGAATCTTCCTT+CGG | 0.523873 | 4.4:-63367904 | None:intergenic |
| TTCTTCAGAGAGTGCATCAG+TGG | 0.529288 | 4.4:+63367753 | MS.gene065953:CDS |
| TTATTCTTTCCAGGAGCAGA+AGG | 0.530019 | 4.4:+63369125 | MS.gene065953:intron |
| CTTACAGGAGGAATTACTCA+TGG | 0.556408 | 4.4:+63368451 | MS.gene065953:intron |
| TGGCGAAACTGAAGATAAGA+TGG | 0.563353 | 4.4:-63369328 | None:intergenic |
| TTATCTTCAGTTTCGCCATG+TGG | 0.585861 | 4.4:+63369333 | MS.gene065953:CDS |
| GAGCAGTTTCTAGTCAAGCT+GGG | 0.589240 | 4.4:-63369394 | None:intergenic |
| CTATAGACGACTGTTGGGAA+AGG | 0.593110 | 4.4:+63368076 | MS.gene065953:intron |
| AGGAGTTCTTTCTTTCCACA+TGG | 0.598924 | 4.4:-63369348 | None:intergenic |
| CTTAACACCATTATCTACAA+AGG | 0.604124 | 4.4:-63369368 | None:intergenic |
| ATCATCCGTGAAGGAGAGGA+AGG | 0.607253 | 4.4:+63368160 | MS.gene065953:CDS |
| GATTATCATCCGTGAAGGAG+AGG | 0.609896 | 4.4:+63368156 | MS.gene065953:CDS |
| TTCAGAGAGTGCATCAGTGG+AGG | 0.615743 | 4.4:+63367756 | MS.gene065953:CDS |
| CGAGGGATTATCATCCGTGA+AGG | 0.619271 | 4.4:+63368151 | MS.gene065953:CDS |
| TCCGAAGGAAGATTCTAGGA+TGG | 0.625002 | 4.4:+63367903 | MS.gene065953:CDS |
| CTAGAGCAGCAAATTAACGA+GGG | 0.653295 | 4.4:+63368134 | MS.gene065953:CDS |
| TGTTCATTAGATACTTCCGA+AGG | 0.661567 | 4.4:+63367888 | MS.gene065953:intron |
| TCTAGAGCAGCAAATTAACG+AGG | 0.688872 | 4.4:+63368133 | MS.gene065953:CDS |
| AGTGGAGGAATATAAAACAG+AGG | 0.706403 | 4.4:+63367771 | MS.gene065953:CDS |
| ATAATACCCTAATTGCAATG+TGG | 0.720756 | 4.4:-63369440 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATATCAAGAAAAATACA+TGG | - | chr4.4:63368725-63368744 | None:intergenic | 20.0% |
| !! | TAAAACAGAAAAAACAAAAG+AGG | - | chr4.4:63368870-63368889 | None:intergenic | 20.0% |
| !!! | TTAATACGATTTTAATCTTC+TGG | + | chr4.4:63369193-63369212 | MS.gene065953:intron | 20.0% |
| ! | AAAGACGAAAAAATAGGAAA+GGG | - | chr4.4:63368198-63368217 | None:intergenic | 25.0% |
| ! | AAATCAAAAAGGAGAAGAAA+AGG | - | chr4.4:63368901-63368920 | None:intergenic | 25.0% |
| ! | AAATCAGTAGTTAACATTTG+AGG | + | chr4.4:63368508-63368527 | MS.gene065953:intron | 25.0% |
| ! | AATCAAAAAGGAGAAGAAAA+GGG | - | chr4.4:63368900-63368919 | None:intergenic | 25.0% |
| ! | GGATAAGAAAGAAATCAAAA+AGG | - | chr4.4:63368912-63368931 | None:intergenic | 25.0% |
| ! | TTGCAATGACATATAAATTC+TGG | + | chr4.4:63368686-63368705 | MS.gene065953:intron | 25.0% |
| !! | TTGTTTGATTTATTCTTTCC+AGG | + | chr4.4:63369116-63369135 | MS.gene065953:intron | 25.0% |
| AAGAACTCCTTTGTAGATAA+TGG | + | chr4.4:63369361-63369380 | MS.gene065953:CDS | 30.0% | |
| AATAGGAAAGGGTTAAGATA+AGG | - | chr4.4:63368187-63368206 | None:intergenic | 30.0% | |
| AGAAATATTGCCTACCAAAT+AGG | - | chr4.4:63368643-63368662 | None:intergenic | 30.0% | |
| AGTTAGAACAATTGTTATGC+AGG | - | chr4.4:63367866-63367885 | None:intergenic | 30.0% | |
| ATCAAGAAAAATACATGGAC+TGG | - | chr4.4:63368720-63368739 | None:intergenic | 30.0% | |
| CTTAACACCATTATCTACAA+AGG | - | chr4.4:63369371-63369390 | None:intergenic | 30.0% | |
| GAAAGACGAAAAAATAGGAA+AGG | - | chr4.4:63368199-63368218 | None:intergenic | 30.0% | |
| ! | AAACCACAACTTAATTTTGG+TGG | - | chr4.4:63368387-63368406 | None:intergenic | 30.0% |
| ! | AAATCTGATAATGCTAAAGC+AGG | - | chr4.4:63368787-63368806 | None:intergenic | 30.0% |
| ! | ACTTTGAGAATAAGCCTATT+TGG | + | chr4.4:63368626-63368645 | MS.gene065953:intron | 30.0% |
| ! | CAGAAACCACAACTTAATTT+TGG | - | chr4.4:63368390-63368409 | None:intergenic | 30.0% |
| !! | AACCACAACTTAATTTTGGT+GGG | - | chr4.4:63368386-63368405 | None:intergenic | 30.0% |
| !!! | TTAACATTTGAGGTTTTGAG+TGG | + | chr4.4:63368518-63368537 | MS.gene065953:intron | 30.0% |
| AACAGGCAATTTGTAAAGGA+TGG | + | chr4.4:63368296-63368315 | MS.gene065953:intron | 35.0% | |
| AGTGGAGGAATATAAAACAG+AGG | + | chr4.4:63367771-63367790 | MS.gene065953:CDS | 35.0% | |
| ATTTGCATTACTGCGCAATA+AGG | + | chr4.4:63368022-63368041 | MS.gene065953:intron | 35.0% | |
| CGCAGTAATGCAAATACAAA+TGG | - | chr4.4:63368017-63368036 | None:intergenic | 35.0% | |
| GAAACAAACTGACAAAACAG+AGG | - | chr4.4:63368973-63368992 | None:intergenic | 35.0% | |
| GAACAGATGTCAAGGATTAA+AGG | - | chr4.4:63368432-63368451 | None:intergenic | 35.0% | |
| GGGAAGAAAGACGAAAAAAT+AGG | - | chr4.4:63368204-63368223 | None:intergenic | 35.0% | |
| TCGAAAAAGCAAAGAACAGA+AGG | - | chr4.4:63368933-63368952 | None:intergenic | 35.0% | |
| TGAGAATAAGCCTATTTGGT+AGG | + | chr4.4:63368630-63368649 | MS.gene065953:intron | 35.0% | |
| TGTTCATTAGATACTTCCGA+AGG | + | chr4.4:63367888-63367907 | MS.gene065953:intron | 35.0% | |
| ! | CTTATCTGGATTGGGAATAA+AGG | + | chr4.4:63368100-63368119 | MS.gene065953:CDS | 35.0% |
| !! | ACCACAACTTAATTTTGGTG+GGG | - | chr4.4:63368385-63368404 | None:intergenic | 35.0% |
| !! | GTGGTTATTGGCTTAGTTAT+AGG | + | chr4.4:63368537-63368556 | MS.gene065953:intron | 35.0% |
| !!! | TTGAGGTTTTGAGTGGTTAT+TGG | + | chr4.4:63368525-63368544 | MS.gene065953:intron | 35.0% |
| ACCATCCTAGAATCTTCCTT+CGG | - | chr4.4:63367907-63367926 | None:intergenic | 40.0% | |
| AGGAGTTCTTTCTTTCCACA+TGG | - | chr4.4:63369351-63369370 | None:intergenic | 40.0% | |
| CCTTTACAAATTGCCTGTTG+CGG | - | chr4.4:63368295-63368314 | None:intergenic | 40.0% | |
| CTAGAGCAGCAAATTAACGA+GGG | + | chr4.4:63368134-63368153 | MS.gene065953:CDS | 40.0% | |
| CTGACAAAACAGAGGAAAAG+AGG | - | chr4.4:63368965-63368984 | None:intergenic | 40.0% | |
| CTTACAGGAGGAATTACTCA+TGG | + | chr4.4:63368451-63368470 | MS.gene065953:intron | 40.0% | |
| CTTCAATCTGCAGTAGAGAT+TGG | - | chr4.4:63369270-63369289 | None:intergenic | 40.0% | |
| GAACAATTGTTATGCAGGCA+AGG | - | chr4.4:63367861-63367880 | None:intergenic | 40.0% | |
| GAATGAAACTTTGCGAAGAC+AGG | + | chr4.4:63369158-63369177 | MS.gene065953:CDS | 40.0% | |
| GATCGTATACGAGAAGAGAA+AGG | - | chr4.4:63368999-63369018 | None:intergenic | 40.0% | |
| GGAAAGGACTTATCTGGATT+GGG | + | chr4.4:63368092-63368111 | MS.gene065953:CDS | 40.0% | |
| GGAATTACTCATGGAACAAC+TGG | + | chr4.4:63368460-63368479 | MS.gene065953:CDS | 40.0% | |
| GGCATAACATGTTCTGTCAT+TGG | - | chr4.4:63369310-63369329 | None:intergenic | 40.0% | |
| TACTTCCGAAGGAAGATTCT+AGG | + | chr4.4:63367899-63367918 | MS.gene065953:CDS | 40.0% | |
| TCAGACACCACATTGCAATT+AGG | + | chr4.4:63369433-63369452 | MS.gene065953:CDS | 40.0% | |
| TCCTGTAAGAACAGATGTCA+AGG | - | chr4.4:63368440-63368459 | None:intergenic | 40.0% | |
| TCCTTGACATCTGTTCTTAC+AGG | + | chr4.4:63368436-63368455 | MS.gene065953:intron | 40.0% | |
| TGGCGAAACTGAAGATAAGA+TGG | - | chr4.4:63369331-63369350 | None:intergenic | 40.0% | |
| TGTGACTATAGACGACTGTT+GGG | + | chr4.4:63368071-63368090 | MS.gene065953:intron | 40.0% | |
| TTATCTTCAGTTTCGCCATG+TGG | + | chr4.4:63369333-63369352 | MS.gene065953:CDS | 40.0% | |
| TTATTCTTTCCAGGAGCAGA+AGG | + | chr4.4:63369125-63369144 | MS.gene065953:intron | 40.0% | |
| TTCTCTCTTCTGTGAAGCAA+GGG | + | chr4.4:63369055-63369074 | MS.gene065953:intron | 40.0% | |
| TTGACATCTGTTCTTACAGG+AGG | + | chr4.4:63368439-63368458 | MS.gene065953:intron | 40.0% | |
| TTGTGACTATAGACGACTGT+TGG | + | chr4.4:63368070-63368089 | MS.gene065953:intron | 40.0% | |
| ! | ACCCCACCAAAATTAAGTTG+TGG | + | chr4.4:63368381-63368400 | MS.gene065953:intron | 40.0% |
| ! | TCTAGAGCAGCAAATTAACG+AGG | + | chr4.4:63368133-63368152 | MS.gene065953:CDS | 40.0% |
| CCGCAACAGGCAATTTGTAA+AGG | + | chr4.4:63368292-63368311 | MS.gene065953:intron | 45.0% | |
| CTATAGACGACTGTTGGGAA+AGG | + | chr4.4:63368076-63368095 | MS.gene065953:intron | 45.0% | |
| CTGTTGGGAAAGGACTTATC+TGG | + | chr4.4:63368086-63368105 | MS.gene065953:CDS | 45.0% | |
| CTTCTCTCTTCTGTGAAGCA+AGG | + | chr4.4:63369054-63369073 | MS.gene065953:intron | 45.0% | |
| CTTCTGTGAAGCAAGGGAAA+GGG | + | chr4.4:63369061-63369080 | MS.gene065953:intron | 45.0% | |
| GATATTTGACAACGCTGGTG+AGG | - | chr4.4:63368230-63368249 | None:intergenic | 45.0% | |
| GATTATCATCCGTGAAGGAG+AGG | + | chr4.4:63368156-63368175 | MS.gene065953:CDS | 45.0% | |
| GGGAAAGGACTTATCTGGAT+TGG | + | chr4.4:63368091-63368110 | MS.gene065953:CDS | 45.0% | |
| GTCAAATATCAGCCATGCCA+CGG | + | chr4.4:63368240-63368259 | MS.gene065953:intron | 45.0% | |
| TCTTCTGTGAAGCAAGGGAA+AGG | + | chr4.4:63369060-63369079 | MS.gene065953:intron | 45.0% | |
| TGGCTGATATTTGACAACGC+TGG | - | chr4.4:63368235-63368254 | None:intergenic | 45.0% | |
| TTGCAGATGGCAGACCATTT+CGG | - | chr4.4:63368348-63368367 | None:intergenic | 45.0% | |
| ! | CAGAATTTGACTTCCGCAAC+AGG | + | chr4.4:63368279-63368298 | MS.gene065953:intron | 45.0% |
| ! | GCAGTGTTATCGATTGCAGA+TGG | - | chr4.4:63368361-63368380 | None:intergenic | 45.0% |
| ! | TCCGAAGGAAGATTCTAGGA+TGG | + | chr4.4:63367903-63367922 | MS.gene065953:CDS | 45.0% |
| ! | TTCTTCAGAGAGTGCATCAG+TGG | + | chr4.4:63367753-63367772 | MS.gene065953:CDS | 45.0% |
| !! | GAGCAGTTTCTAGTCAAGCT+GGG | - | chr4.4:63369397-63369416 | None:intergenic | 45.0% |
| !! | TGAGCAGTTTCTAGTCAAGC+TGG | - | chr4.4:63369398-63369417 | None:intergenic | 45.0% |
| AAGGACCTTCCTCTCCTTCA+CGG | - | chr4.4:63368168-63368187 | None:intergenic | 50.0% | |
| ACTGGAGCAGTCTCGAATAC+AGG | + | chr4.4:63368478-63368497 | MS.gene065953:CDS | 50.0% | |
| ATCATCCGTGAAGGAGAGGA+AGG | + | chr4.4:63368160-63368179 | MS.gene065953:CDS | 50.0% | |
| ATTTGACAACGCTGGTGAGG+TGG | - | chr4.4:63368227-63368246 | None:intergenic | 50.0% | |
| CATGCCACGGCTTATTGCAT+GGG | + | chr4.4:63368253-63368272 | MS.gene065953:intron | 50.0% | |
| CGAGGGATTATCATCCGTGA+AGG | + | chr4.4:63368151-63368170 | MS.gene065953:CDS | 50.0% | |
| GGATTCCAGGCTTTCCGAAA+TGG | + | chr4.4:63368331-63368350 | MS.gene065953:intron | 50.0% | |
| TTTGACAACGCTGGTGAGGT+GGG | - | chr4.4:63368226-63368245 | None:intergenic | 50.0% | |
| ! | TTCAGAGAGTGCATCAGTGG+AGG | + | chr4.4:63367756-63367775 | MS.gene065953:CDS | 50.0% |
| AAAGCCTGGAATCCGCCATG+GGG | - | chr4.4:63368325-63368344 | None:intergenic | 55.0% | |
| CCATGCAATAAGCCGTGGCA+TGG | - | chr4.4:63368255-63368274 | None:intergenic | 55.0% | |
| CCATGCCACGGCTTATTGCA+TGG | + | chr4.4:63368252-63368271 | MS.gene065953:intron | 55.0% | |
| GAAAGCCTGGAATCCGCCAT+GGG | - | chr4.4:63368326-63368345 | None:intergenic | 55.0% | |
| GCAGACCATTTCGGAAAGCC+TGG | - | chr4.4:63368339-63368358 | None:intergenic | 55.0% | |
| TGTAAAGGATGGCAGCCCCA+TGG | + | chr4.4:63368307-63368326 | MS.gene065953:intron | 55.0% | |
| TTGACAACGCTGGTGAGGTG+GGG | - | chr4.4:63368225-63368244 | None:intergenic | 55.0% | |
| AAAGGATGGCAGCCCCATGG+CGG | + | chr4.4:63368310-63368329 | MS.gene065953:intron | 60.0% | |
| CCAGCATAGCCTTCTGCTCC+TGG | - | chr4.4:63369137-63369156 | None:intergenic | 60.0% | |
| CCAGGAGCAGAAGGCTATGC+TGG | + | chr4.4:63369134-63369153 | MS.gene065953:intron | 60.0% | |
| GGAAAGCCTGGAATCCGCCA+TGG | - | chr4.4:63368327-63368346 | None:intergenic | 60.0% | |
| GTCGCCCATGCAATAAGCCG+TGG | - | chr4.4:63368260-63368279 | None:intergenic | 60.0% | |
| TGACAACGCTGGTGAGGTGG+GGG | - | chr4.4:63368224-63368243 | None:intergenic | 60.0% | |
| !! | GCAGCCCCATGGCGGATTCC+AGG | + | chr4.4:63368318-63368337 | MS.gene065953:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 63367714 | 63369455 | 63367714 | ID=MS.gene065953 |
| chr4.4 | mRNA | 63367714 | 63369455 | 63367714 | ID=MS.gene065953.t1;Parent=MS.gene065953 |
| chr4.4 | exon | 63367714 | 63367792 | 63367714 | ID=MS.gene065953.t1.exon1;Parent=MS.gene065953.t1 |
| chr4.4 | CDS | 63367714 | 63367792 | 63367714 | ID=cds.MS.gene065953.t1;Parent=MS.gene065953.t1 |
| chr4.4 | exon | 63367898 | 63367971 | 63367898 | ID=MS.gene065953.t1.exon2;Parent=MS.gene065953.t1 |
| chr4.4 | CDS | 63367898 | 63367971 | 63367898 | ID=cds.MS.gene065953.t1;Parent=MS.gene065953.t1 |
| chr4.4 | exon | 63368082 | 63368181 | 63368082 | ID=MS.gene065953.t1.exon3;Parent=MS.gene065953.t1 |
| chr4.4 | CDS | 63368082 | 63368181 | 63368082 | ID=cds.MS.gene065953.t1;Parent=MS.gene065953.t1 |
| chr4.4 | exon | 63368458 | 63368499 | 63368458 | ID=MS.gene065953.t1.exon4;Parent=MS.gene065953.t1 |
| chr4.4 | CDS | 63368458 | 63368499 | 63368458 | ID=cds.MS.gene065953.t1;Parent=MS.gene065953.t1 |
| chr4.4 | exon | 63369138 | 63369179 | 63369138 | ID=MS.gene065953.t1.exon5;Parent=MS.gene065953.t1 |
| chr4.4 | CDS | 63369138 | 63369179 | 63369138 | ID=cds.MS.gene065953.t1;Parent=MS.gene065953.t1 |
| chr4.4 | exon | 63369283 | 63369455 | 63369283 | ID=MS.gene065953.t1.exon6;Parent=MS.gene065953.t1 |
| chr4.4 | CDS | 63369283 | 63369455 | 63369283 | ID=cds.MS.gene065953.t1;Parent=MS.gene065953.t1 |
| Gene Sequence |
| Protein sequence |
