AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06599

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06599.t1 MTR_3g092890 96.104 154 6 0 1 154 1 154 7.39e-108 311
MS.gene06599.t1 MTR_8g024120 57.246 138 55 2 15 148 18 155 1.39e-51 167
MS.gene06599.t1 MTR_1g070135 38.816 152 89 2 1 148 1 152 1.49e-33 120
MS.gene06599.t1 MTR_1g070135 38.816 152 89 2 1 148 1 152 4.10e-33 120
MS.gene06599.t1 MTR_7g081710 41.135 141 78 2 13 148 23 163 3.94e-32 117
MS.gene06599.t1 MTR_1g062110 38.854 157 86 4 4 152 29 183 4.01e-32 117
MS.gene06599.t1 MTR_1g062110 38.854 157 86 4 4 152 29 183 9.33e-32 117
MS.gene06599.t1 MTR_7g102820 38.776 147 81 2 14 151 37 183 9.50e-30 112
MS.gene06599.t1 MTR_7g016090 36.601 153 95 2 1 151 1 153 1.10e-29 110
MS.gene06599.t1 MTR_7g016090 36.601 153 95 2 1 151 3 155 1.12e-29 110
MS.gene06599.t1 MTR_1g070120 37.931 145 84 3 8 148 10 152 1.52e-29 110
MS.gene06599.t1 MTR_1g048940 35.461 141 84 2 13 148 22 160 2.96e-26 102
MS.gene06599.t1 MTR_7g016080 36.129 155 95 3 1 151 1 155 3.05e-26 101
MS.gene06599.t1 MTR_1g070085 34.375 160 101 2 4 159 3 162 1.31e-25 100
MS.gene06599.t1 MTR_1g070085 34.375 160 101 2 4 159 3 162 1.31e-25 100
MS.gene06599.t1 MTR_1g070080 37.415 147 87 3 4 145 3 149 3.17e-25 99.0
MS.gene06599.t1 MTR_5g082750 37.931 145 81 4 15 151 25 168 2.06e-24 97.1
MS.gene06599.t1 MTR_3g070860 37.662 154 86 5 6 151 18 169 2.21e-24 97.1
MS.gene06599.t1 MTR_5g032880 37.037 135 78 3 15 142 353 487 7.14e-21 88.6
MS.gene06599.t1 MTR_5g032880 38.636 132 76 3 15 144 15 143 9.26e-19 82.4
MS.gene06599.t1 MTR_7g068650 32.237 152 88 5 13 151 24 173 1.90e-20 86.7
MS.gene06599.t1 MTR_6g015950 31.169 154 103 2 1 151 1 154 3.19e-20 85.5
MS.gene06599.t1 MTR_3g043900 36.842 133 79 2 15 143 29 160 4.21e-20 85.5
MS.gene06599.t1 MTR_4g015790 32.934 167 84 6 8 151 20 181 5.52e-20 85.1
MS.gene06599.t1 MTR_3g045230 36.134 119 72 1 15 129 29 147 6.49e-20 85.1
MS.gene06599.t1 MTR_7g102720 39.362 94 57 0 36 129 35 128 5.32e-19 82.4
MS.gene06599.t1 MTR_5g032870 35.507 138 76 3 15 142 15 149 1.28e-18 81.3
MS.gene06599.t1 MTR_7g045650 31.690 142 92 4 14 151 35 175 1.01e-17 78.6
MS.gene06599.t1 MTR_3g091350 37.097 124 65 5 37 152 58 176 1.65e-17 78.6
MS.gene06599.t1 MTR_3g058610 31.847 157 98 5 3 152 4 158 2.67e-17 77.8
MS.gene06599.t1 MTR_7g063730 33.088 136 86 4 14 145 39 173 5.91e-17 77.0
MS.gene06599.t1 MTR_5g065010 32.026 153 94 3 3 149 4 152 1.01e-16 75.9
MS.gene06599.t1 MTR_2g069300 40.860 93 50 1 38 125 54 146 1.04e-16 76.3
MS.gene06599.t1 MTR_3g091360 35.484 124 69 4 37 153 51 170 1.43e-16 75.9
MS.gene06599.t1 MTR_8g075890 30.556 144 93 3 15 152 16 158 2.73e-16 75.1
MS.gene06599.t1 MTR_5g055820 33.803 142 84 5 13 148 22 159 2.78e-16 74.7
MS.gene06599.t1 MTR_1g034140 31.757 148 78 4 18 148 7 148 2.80e-16 74.7
MS.gene06599.t1 MTR_5g022020 28.846 156 102 5 3 151 4 157 4.02e-16 74.3
MS.gene06599.t1 MTR_7g062570 31.507 146 95 4 10 151 26 170 4.37e-16 74.3
MS.gene06599.t1 MTR_7g062600 31.469 143 91 5 14 151 26 166 4.64e-16 74.3
MS.gene06599.t1 MTR_7g062550 31.690 142 92 4 14 151 35 175 5.62e-16 74.3
MS.gene06599.t1 MTR_0032s0040 31.852 135 78 5 5 127 31 163 1.99e-15 72.8
MS.gene06599.t1 MTR_8g075830 29.861 144 94 3 15 152 16 158 3.04e-15 72.0
MS.gene06599.t1 MTR_4g099380 32.824 131 84 2 5 133 141 269 3.10e-15 71.2
MS.gene06599.t1 MTR_5g059130 33.562 146 88 5 13 152 18 160 3.35e-15 71.6
MS.gene06599.t1 MTR_5g048550 31.008 129 84 3 14 139 23 149 3.38e-15 72.0
MS.gene06599.t1 MTR_5g048850 33.571 140 84 5 13 147 22 157 3.68e-15 71.2
MS.gene06599.t1 MTR_3g072820 30.282 142 94 4 15 152 18 158 4.60e-15 71.2
MS.gene06599.t1 MTR_4g087090 31.788 151 75 6 19 148 12 155 5.30e-15 71.2
MS.gene06599.t1 MTR_5g048850 33.813 139 83 5 13 146 22 156 6.32e-15 71.2
MS.gene06599.t1 MTR_7g062580 30.070 143 93 5 14 151 30 170 7.02e-15 70.9
MS.gene06599.t1 MTR_3g063180 30.380 158 98 6 5 154 13 166 9.46e-15 70.5
MS.gene06599.t1 MTR_2g069190 35.484 93 55 1 38 125 68 160 1.11e-14 70.5
MS.gene06599.t1 MTR_5g055690 28.571 126 88 1 13 136 22 147 1.27e-14 69.7
MS.gene06599.t1 MTR_1g032140 32.061 131 83 3 8 134 36 164 1.73e-14 70.1
MS.gene06599.t1 MTR_4g087100 32.716 162 82 7 13 151 2 159 1.79e-14 69.7
MS.gene06599.t1 MTR_2g069210 33.663 101 62 1 38 133 64 164 1.83e-14 69.7
MS.gene06599.t1 MTR_1g034060 38.384 99 49 3 14 101 3 100 1.83e-14 69.7
MS.gene06599.t1 MTR_1g034120 38.384 99 49 3 14 101 3 100 1.94e-14 69.7
MS.gene06599.t1 MTR_5g055690 27.737 137 93 2 13 143 22 158 2.30e-14 69.7
MS.gene06599.t1 MTR_3g072810 32.624 141 90 4 14 151 16 154 2.64e-14 69.3
MS.gene06599.t1 MTR_8g028435 34.314 102 61 1 38 133 60 161 2.84e-14 69.3
MS.gene06599.t1 MTR_2g068850 32.609 138 80 4 14 143 32 164 2.95e-14 67.8
MS.gene06599.t1 MTR_4g093840 28.873 142 88 6 4 133 24 164 3.25e-14 69.3
MS.gene06599.t1 MTR_1g032250 29.577 142 94 3 5 142 40 179 3.91e-14 68.9
MS.gene06599.t1 MTR_8g009110 32.110 109 68 1 38 140 60 168 4.47e-14 68.9
MS.gene06599.t1 MTR_7g029655 33.835 133 67 5 8 127 34 158 5.45e-14 68.6
MS.gene06599.t1 MTR_8g009160 33.333 102 62 1 38 133 60 161 5.98e-14 68.6
MS.gene06599.t1 MTR_2g069090 33.333 93 57 1 38 125 68 160 7.82e-14 68.2
MS.gene06599.t1 MTR_2g068840 32.624 141 76 4 14 143 32 164 8.93e-14 67.8
MS.gene06599.t1 MTR_3g088565 36.441 118 57 6 38 148 3 109 1.04e-13 67.4
MS.gene06599.t1 MTR_8g041600 36.735 98 55 1 37 127 67 164 1.79e-13 66.6
MS.gene06599.t1 MTR_5g048470 35.862 145 79 8 13 148 27 166 2.20e-13 66.6
MS.gene06599.t1 MTR_1g032220 28.873 142 95 3 5 142 40 179 2.26e-13 66.6
MS.gene06599.t1 MTR_8g041600 36.735 98 55 1 37 127 67 164 2.52e-13 66.6
MS.gene06599.t1 MTR_1g093120 30.435 138 85 3 5 133 32 167 3.16e-13 66.2
MS.gene06599.t1 MTR_8g009150 32.110 109 68 1 38 140 60 168 3.17e-13 66.2
MS.gene06599.t1 MTR_8g064200 34.646 127 73 5 13 133 27 149 3.36e-13 66.2
MS.gene06599.t1 MTR_8g064200 34.646 127 73 5 13 133 32 154 3.41e-13 66.2
MS.gene06599.t1 MTR_8g009120 32.110 109 68 1 38 140 60 168 3.63e-13 66.2
MS.gene06599.t1 MTR_5g059140 28.395 162 104 6 1 152 1 160 4.62e-13 65.9
MS.gene06599.t1 MTR_1g070185 32.847 137 84 4 5 135 37 171 5.69e-13 65.5
MS.gene06599.t1 MTR_0009s0410 29.771 131 85 4 13 139 21 148 5.95e-13 63.2
MS.gene06599.t1 MTR_2g033270 37.234 94 54 2 37 130 18 106 6.33e-13 65.5
MS.gene06599.t1 MTR_1g093140 27.660 141 90 3 5 133 33 173 7.58e-13 65.5
MS.gene06599.t1 MTR_4g087120 33.613 119 66 4 18 127 7 121 7.65e-13 65.1
MS.gene06599.t1 MTR_8g062600 32.353 102 64 1 37 133 66 167 8.26e-13 65.1
MS.gene06599.t1 MTR_0009s0050 29.286 140 90 3 14 151 23 155 8.46e-13 65.1
MS.gene06599.t1 MTR_2g072890 28.986 138 93 2 6 139 20 156 8.71e-13 65.1
MS.gene06599.t1 MTR_0009s0050 29.286 140 90 3 14 151 23 155 9.38e-13 65.1
MS.gene06599.t1 MTR_5g055680 31.707 123 79 3 14 133 23 143 1.10e-12 64.7
MS.gene06599.t1 MTR_8g009170 30.275 109 70 1 38 140 60 168 1.13e-12 64.3
MS.gene06599.t1 MTR_8g009170 30.275 109 70 1 38 140 60 168 1.44e-12 64.3
MS.gene06599.t1 MTR_4g100590 35.345 116 64 4 37 141 8 123 1.59e-12 63.9
MS.gene06599.t1 MTR_0328s0030 29.286 140 93 4 13 148 20 157 1.61e-12 64.3
MS.gene06599.t1 MTR_7g105070 30.216 139 89 4 1 133 22 158 1.73e-12 64.3
MS.gene06599.t1 MTR_4g100600 45.161 62 34 0 37 98 8 69 2.11e-12 63.2
MS.gene06599.t1 MTR_4g024660 27.397 146 98 3 13 153 42 184 2.24e-12 63.9
MS.gene06599.t1 MTR_2g068960 29.197 137 90 3 13 142 36 172 2.46e-12 63.9
MS.gene06599.t1 MTR_4g100600 45.161 62 34 0 37 98 8 69 3.27e-12 63.2
MS.gene06599.t1 MTR_1g093110 30.216 139 86 3 5 133 33 170 3.38e-12 63.5
MS.gene06599.t1 MTR_4g087140 37.895 95 49 3 17 102 6 99 3.47e-12 63.2
MS.gene06599.t1 MTR_5g055800 27.642 123 86 2 14 134 23 144 4.90e-12 62.8
MS.gene06599.t1 MTR_2g068950 31.532 111 71 2 37 142 62 172 5.81e-12 62.8
MS.gene06599.t1 MTR_0009s0480 28.800 125 84 3 13 134 21 143 5.99e-12 62.0
MS.gene06599.t1 MTR_8g041620 34.694 98 57 1 37 127 67 164 8.01e-12 62.4
MS.gene06599.t1 MTR_5g011250 28.302 159 97 7 8 152 14 169 9.09e-12 62.0
MS.gene06599.t1 MTR_0009s0480 28.800 125 84 3 13 134 21 143 9.38e-12 62.0
MS.gene06599.t1 MTR_8g009090 32.979 94 57 1 38 125 60 153 9.80e-12 62.0
MS.gene06599.t1 MTR_5g048740 26.531 147 97 4 2 139 3 147 1.05e-11 62.0
MS.gene06599.t1 MTR_2g072930 26.718 131 91 2 13 139 26 155 1.69e-11 59.7
MS.gene06599.t1 MTR_8g008930 32.039 103 63 2 38 133 60 162 1.79e-11 61.2
MS.gene06599.t1 MTR_5g055900 32.609 92 58 2 13 101 22 112 2.36e-11 60.8
MS.gene06599.t1 MTR_2g437380 26.429 140 90 6 15 145 28 163 2.92e-11 60.8
MS.gene06599.t1 MTR_4g116010 32.743 113 74 2 38 149 50 161 3.49e-11 60.5
MS.gene06599.t1 MTR_4g087130 32.203 118 70 3 18 126 7 123 3.81e-11 60.5
MS.gene06599.t1 MTR_1g033810 28.358 134 90 3 5 133 13 145 4.10e-11 60.5
MS.gene06599.t1 MTR_2g102570 29.323 133 87 4 14 144 27 154 4.48e-11 60.1
MS.gene06599.t1 MTR_8g009080 32.000 100 61 2 38 132 94 191 6.21e-11 59.7
MS.gene06599.t1 MTR_7g062560 30.657 137 73 6 15 142 38 161 7.19e-11 59.7
MS.gene06599.t1 MTR_8g009080 32.000 100 61 2 38 132 94 191 7.78e-11 59.3
MS.gene06599.t1 MTR_4g100610 45.161 62 34 0 37 98 8 69 7.80e-11 58.5
MS.gene06599.t1 MTR_8g009080 32.000 100 61 2 38 132 94 191 8.47e-11 59.3
MS.gene06599.t1 MTR_1g043760 25.850 147 97 4 5 146 23 162 8.84e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06599.t1 AT5G24530 61.589 151 58 0 1 151 1 151 1.14e-66 206
MS.gene06599.t1 AT4G10500 52.318 151 65 3 6 152 14 161 1.92e-49 162
MS.gene06599.t1 AT4G10490 52.113 142 64 2 15 152 18 159 4.07e-47 156
MS.gene06599.t1 AT2G36690 42.069 145 75 2 13 148 30 174 2.83e-33 120
MS.gene06599.t1 AT2G36690 42.069 145 75 2 13 148 30 174 2.83e-33 120
MS.gene06599.t1 AT2G44800 38.298 141 81 2 14 148 26 166 1.55e-29 110
MS.gene06599.t1 AT3G60290 40.714 140 77 3 15 148 27 166 2.96e-29 110
MS.gene06599.t1 AT5G07480 35.971 139 81 2 15 148 24 159 6.25e-25 98.6
MS.gene06599.t1 AT3G13610 39.316 117 69 2 15 129 38 154 4.82e-22 90.9
MS.gene06599.t1 AT5G12270 33.566 143 93 2 3 143 21 163 1.05e-20 87.0
MS.gene06599.t1 AT5G05600 37.162 148 82 5 13 151 36 181 2.04e-20 85.5
MS.gene06599.t1 AT5G05600 37.162 148 82 5 13 151 36 181 6.10e-20 85.1
MS.gene06599.t1 AT3G11180 39.161 143 75 5 13 144 65 206 8.86e-20 85.1
MS.gene06599.t1 AT3G11180 37.162 148 84 5 13 152 65 211 9.46e-20 85.1
MS.gene06599.t1 AT1G55290 34.351 131 83 2 15 142 37 167 2.75e-19 83.2
MS.gene06599.t1 AT3G51240 31.507 146 93 3 13 152 13 157 3.92e-18 80.1
MS.gene06599.t1 AT1G06620 36.275 102 60 1 37 133 61 162 1.42e-16 75.1
MS.gene06599.t1 AT3G55970 31.724 145 85 5 13 145 23 165 2.32e-16 74.7
MS.gene06599.t1 AT1G06620 36.275 102 60 1 37 133 61 162 2.83e-16 75.1
MS.gene06599.t1 AT2G38240 29.787 141 94 4 15 151 23 162 3.38e-16 74.7
MS.gene06599.t1 AT3G55970 32.168 143 83 5 15 145 25 165 4.74e-16 74.3
MS.gene06599.t1 AT3G12900 33.099 142 89 3 5 142 25 164 4.84e-16 74.3
MS.gene06599.t1 AT5G08640 28.289 152 104 4 5 152 9 159 5.18e-16 73.9
MS.gene06599.t1 AT5G08640 28.289 152 104 4 5 152 9 159 5.18e-16 73.9
MS.gene06599.t1 AT1G06640 32.609 138 79 4 5 132 29 162 2.16e-15 72.0
MS.gene06599.t1 AT1G06640 32.609 138 79 4 5 132 29 162 2.25e-15 72.0
MS.gene06599.t1 AT1G06640 32.609 138 79 4 5 132 29 162 3.30e-15 72.0
MS.gene06599.t1 AT3G19000 30.120 166 83 6 13 151 2 161 1.49e-14 69.3
MS.gene06599.t1 AT1G06650 28.777 139 87 3 5 133 29 165 2.41e-14 69.3
MS.gene06599.t1 AT1G06650 29.771 131 80 3 5 125 29 157 2.82e-14 69.3
MS.gene06599.t1 AT3G19000 30.120 166 83 6 13 151 2 161 3.17e-14 69.3
MS.gene06599.t1 AT2G30830 32.353 102 63 1 38 133 54 155 4.35e-14 68.9
MS.gene06599.t1 AT4G16330 30.833 120 76 4 37 151 66 183 4.90e-14 68.6
MS.gene06599.t1 AT5G43440 35.185 108 66 1 37 140 61 168 5.36e-14 68.2
MS.gene06599.t1 AT5G43440 35.514 107 65 1 38 140 62 168 7.87e-14 68.2
MS.gene06599.t1 AT1G06645 31.298 131 78 3 5 125 42 170 9.65e-14 67.8
MS.gene06599.t1 AT3G19010 43.243 74 38 1 33 102 23 96 1.11e-13 67.4
MS.gene06599.t1 AT1G77330 36.937 111 60 4 38 144 3 107 1.53e-13 67.0
MS.gene06599.t1 AT3G19010 43.243 74 38 1 33 102 23 96 1.62e-13 67.0
MS.gene06599.t1 AT3G19010 43.243 74 38 1 33 102 23 96 1.62e-13 67.0
MS.gene06599.t1 AT3G19010 43.243 74 38 1 33 102 23 96 2.20e-13 66.6
MS.gene06599.t1 AT5G43935 29.600 125 79 4 27 149 12 129 2.83e-13 66.2
MS.gene06599.t1 AT5G63600 31.206 141 78 5 23 152 11 143 3.89e-13 65.9
MS.gene06599.t1 AT5G63600 31.206 141 78 5 23 152 11 143 3.95e-13 65.9
MS.gene06599.t1 AT4G25300 31.496 127 81 3 13 134 26 151 4.75e-13 65.9
MS.gene06599.t1 AT3G21420 30.667 150 88 5 13 149 26 172 5.52e-13 65.9
MS.gene06599.t1 AT3G61400 32.168 143 78 5 5 133 28 165 5.74e-13 65.1
MS.gene06599.t1 AT3G61400 33.645 107 61 1 37 133 59 165 7.01e-13 65.5
MS.gene06599.t1 AT1G04350 32.558 129 80 5 11 133 29 156 7.17e-13 65.5
MS.gene06599.t1 AT5G63595 28.000 125 82 3 30 152 17 135 8.39e-13 64.7
MS.gene06599.t1 AT1G80340 27.972 143 91 3 6 144 18 152 1.29e-12 64.7
MS.gene06599.t1 AT5G63590 29.661 118 81 2 37 152 13 130 2.04e-12 63.9
MS.gene06599.t1 AT1G17010 31.250 128 81 3 13 134 27 153 2.92e-12 63.5
MS.gene06599.t1 AT1G15550 30.657 137 79 4 14 142 29 157 2.93e-12 63.5
MS.gene06599.t1 AT1G03410 31.159 138 84 5 5 133 23 158 3.03e-12 63.5
MS.gene06599.t1 AT1G03410 31.159 138 84 5 5 133 60 195 4.59e-12 63.2
MS.gene06599.t1 AT1G03400 30.928 97 64 1 37 133 55 148 7.82e-12 62.4
MS.gene06599.t1 AT5G54000 30.128 156 93 6 4 148 11 161 8.75e-12 62.4
MS.gene06599.t1 AT5G63580 30.252 119 77 4 36 151 17 132 9.02e-12 61.6
MS.gene06599.t1 AT5G63580 29.752 121 75 4 36 151 17 132 1.00e-11 61.6
MS.gene06599.t1 AT1G03400 30.928 97 64 1 37 133 55 148 1.20e-11 62.0
MS.gene06599.t1 AT1G17020 28.671 143 95 4 13 149 27 168 2.94e-11 60.8
MS.gene06599.t1 AT4G25310 30.645 124 78 3 15 134 29 148 4.48e-11 60.1
MS.gene06599.t1 AT1G04380 39.091 110 58 6 38 145 45 147 4.63e-11 59.7
MS.gene06599.t1 AT4G25310 30.645 124 78 3 15 134 29 148 4.86e-11 60.1
MS.gene06599.t1 AT1G04380 39.091 110 58 6 38 145 45 147 6.39e-11 59.7

Find 30 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGTTCCTATCATTGATT+TGG 0.233266 3.3:+77573504 MS.gene06599:CDS
TTCGGGTCTGATGTAGCTTT+CGG 0.284448 3.3:-77573443 None:intergenic
TCGGGTCTGATGTAGCTTTC+GGG 0.320790 3.3:-77573442 None:intergenic
GGAGGATTAGAAGGCCATTC+TGG 0.349419 3.3:-77574243 None:intergenic
TAGACTTCATTGCTATCCTT+TGG 0.360775 3.3:+77574209 MS.gene06599:CDS
GACACCAAAGTGCTTTCTTC+CGG 0.368362 3.3:+77573400 MS.gene06599:CDS
ACAAAGAGGAGGTGCATAAT+TGG 0.371306 3.3:+77574175 MS.gene06599:CDS
TTGAATGAAGGAGGATTAGA+AGG 0.374736 3.3:-77574252 None:intergenic
AATGTTCCTATCATTGATTT+GGG 0.394239 3.3:+77573505 MS.gene06599:CDS
ACACAGATTGTGCAGCAAAT+TGG 0.405459 3.3:+77573541 MS.gene06599:CDS
AAGACATGGTCTATCAGATT+CGG 0.410372 3.3:-77573461 None:intergenic
AAGGATAGCAATGAAGTCTA+AGG 0.423736 3.3:-77574206 None:intergenic
ATTGGACAGGTAGTTAATCA+TGG 0.442304 3.3:+77574018 MS.gene06599:intron
GGTGAAGCATGCAGCTCCTA+TGG 0.451140 3.3:+77573562 MS.gene06599:CDS
AGACATGGTCTATCAGATTC+GGG 0.453451 3.3:-77573460 None:intergenic
TGGATAATTATGTGCCAGAA+TGG 0.460872 3.3:+77574229 MS.gene06599:CDS
TGAGAACCCAAATCAATGAT+AGG 0.483199 3.3:-77573511 None:intergenic
TTGGACAGGTAGTTAATCAT+GGG 0.500876 3.3:+77574019 MS.gene06599:intron
GATAGTCTCATTGTCTTCGT+GGG 0.526905 3.3:-77574135 None:intergenic
AGATAGTCTCATTGTCTTCG+TGG 0.538477 3.3:-77574136 None:intergenic
GAGTGGACTTACTTGAATGA+AGG 0.551937 3.3:-77574264 None:intergenic
AAGAGGAGGTGCATAATTGG+AGG 0.579585 3.3:+77574178 MS.gene06599:CDS
TGGACTTACTTGAATGAAGG+AGG 0.584641 3.3:-77574261 None:intergenic
GATTCCGGAAGAAAGCACTT+TGG 0.619711 3.3:-77573404 None:intergenic
TTTAGCCACGTATCTATCAA+CGG 0.620363 3.3:+77574313 MS.gene06599:intron
AGAGGAGGTGCATAATTGGA+GGG 0.638458 3.3:+77574179 MS.gene06599:CDS
TCTGGCACATAATTATCCAA+AGG 0.648305 3.3:-77574225 None:intergenic
TGAGAGACTTGAGAAAGACA+TGG 0.680148 3.3:-77573475 None:intergenic
CTTGTCCGTTGATAGATACG+TGG 0.694743 3.3:-77574318 None:intergenic
TGGACAGGTAGTTAATCATG+GGG 0.699574 3.3:+77574020 MS.gene06599:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAAAAAATTGTAATATT+GGG - chr3.3:77573604-77573623 None:intergenic 10.0%
!! TAAACAAAAAATTGTAATAT+TGG - chr3.3:77573605-77573624 None:intergenic 10.0%
!! CCAAAAAAAAAAAAAGAATA+GGG + chr3.3:77573729-77573748 MS.gene06599:intron 15.0%
!! TCCAAAAAAAAAAAAAGAAT+AGG + chr3.3:77573728-77573747 MS.gene06599:intron 15.0%
!! AATTGTATTTAGTAGAAATG+AGG - chr3.3:77573761-77573780 None:intergenic 20.0%
!! ATATTGTTAATTAATTGGAC+AGG + chr3.3:77574005-77574024 MS.gene06599:intron 20.0%
!! CTATTCATTGTAATTAGAAT+AGG + chr3.3:77573692-77573711 MS.gene06599:intron 20.0%
!!! CAATGAAGATTATTTTAGAT+AGG + chr3.3:77573651-77573670 MS.gene06599:intron 20.0%
!!! CCCTATTCTTTTTTTTTTTT+TGG - chr3.3:77573732-77573751 None:intergenic 20.0%
!!! CTTTTTTTTTTTTTGGAGAA+GGG - chr3.3:77573725-77573744 None:intergenic 20.0%
!!! GATAGAATAAAATGTCATTT+TGG + chr3.3:77573874-77573893 MS.gene06599:intron 20.0%
!!! TAATTTTGAATAATGGATTC+CGG - chr3.3:77573422-77573441 None:intergenic 20.0%
!!! TCTTTTTTTTTTTTTGGAGA+AGG - chr3.3:77573726-77573745 None:intergenic 20.0%
! CTATCACTAAAAATTGAACT+GGG - chr3.3:77573859-77573878 None:intergenic 25.0%
! TAATCTTCATTGTTCATCTA+TGG - chr3.3:77573643-77573662 None:intergenic 25.0%
! TCTATCACTAAAAATTGAAC+TGG - chr3.3:77573860-77573879 None:intergenic 25.0%
!! AAATGTTCCTATCATTGATT+TGG + chr3.3:77573504-77573523 MS.gene06599:CDS 25.0%
!! ATATTAAACACCGTAAGTTT+TGG + chr3.3:77573969-77573988 MS.gene06599:intron 25.0%
!! TATTAAACACCGTAAGTTTT+GGG + chr3.3:77573970-77573989 MS.gene06599:intron 25.0%
!!! AAGTTTTAATGTGAACAAAG+AGG + chr3.3:77574161-77574180 MS.gene06599:CDS 25.0%
!!! AATGTTCCTATCATTGATTT+GGG + chr3.3:77573505-77573524 MS.gene06599:CDS 25.0%
!!! GTTTTTTTCAATTCTTCTAG+AGG - chr3.3:77574048-77574067 None:intergenic 25.0%
!!! TTTCGGGTAATTTTGAATAA+TGG - chr3.3:77573429-77573448 None:intergenic 25.0%
ATTGAAAAAAACAGCAGAAG+TGG + chr3.3:77574056-77574075 MS.gene06599:CDS 30.0%
! AATTAGTCTCCCAAAACTTA+CGG - chr3.3:77573982-77574001 None:intergenic 30.0%
! TTTTAATGTGAACAAAGAGG+AGG + chr3.3:77574164-77574183 MS.gene06599:CDS 30.0%
AAGACATGGTCTATCAGATT+CGG - chr3.3:77573464-77573483 None:intergenic 35.0%
AAGGATAGCAATGAAGTCTA+AGG - chr3.3:77574209-77574228 None:intergenic 35.0%
ATTGGACAGGTAGTTAATCA+TGG + chr3.3:77574018-77574037 MS.gene06599:intron 35.0%
TAAAAATTGAACTGGGTTCC+TGG - chr3.3:77573852-77573871 None:intergenic 35.0%
TAGACTTCATTGCTATCCTT+TGG + chr3.3:77574209-77574228 MS.gene06599:CDS 35.0%
TCTGGCACATAATTATCCAA+AGG - chr3.3:77574228-77574247 None:intergenic 35.0%
TGAGAACCCAAATCAATGAT+AGG - chr3.3:77573514-77573533 None:intergenic 35.0%
TGGATAATTATGTGCCAGAA+TGG + chr3.3:77574229-77574248 MS.gene06599:CDS 35.0%
TTGAATGAAGGAGGATTAGA+AGG - chr3.3:77574255-77574274 None:intergenic 35.0%
TTGGACAGGTAGTTAATCAT+GGG + chr3.3:77574019-77574038 MS.gene06599:intron 35.0%
TTTAGCCACGTATCTATCAA+CGG + chr3.3:77574313-77574332 MS.gene06599:intron 35.0%
ACACAGATTGTGCAGCAAAT+TGG + chr3.3:77573541-77573560 MS.gene06599:CDS 40.0%
ACCACTTATGTTACAACCAG+TGG - chr3.3:77573809-77573828 None:intergenic 40.0%
ACGGAGAAAAATGAAGAGAG+TGG - chr3.3:77574284-77574303 None:intergenic 40.0%
AGACATGGTCTATCAGATTC+GGG - chr3.3:77573463-77573482 None:intergenic 40.0%
AGATAGTCTCATTGTCTTCG+TGG - chr3.3:77574139-77574158 None:intergenic 40.0%
CGTGGCTAAAATTAGAGAGA+CGG - chr3.3:77574303-77574322 None:intergenic 40.0%
CTAGACCTGGAAAAAACCAT+AGG - chr3.3:77573581-77573600 None:intergenic 40.0%
GATAGTCTCATTGTCTTCGT+GGG - chr3.3:77574138-77574157 None:intergenic 40.0%
TGAGAGACTTGAGAAAGACA+TGG - chr3.3:77573478-77573497 None:intergenic 40.0%
TGGACAGGTAGTTAATCATG+GGG + chr3.3:77574020-77574039 MS.gene06599:intron 40.0%
TGGACTTACTTGAATGAAGG+AGG - chr3.3:77574264-77574283 None:intergenic 40.0%
! AACTTCATTTTCTCCTCCAC+TGG - chr3.3:77574102-77574121 None:intergenic 40.0%
! ACAAAGAGGAGGTGCATAAT+TGG + chr3.3:77574175-77574194 MS.gene06599:CDS 40.0%
! GAGTGGACTTACTTGAATGA+AGG - chr3.3:77574267-77574286 None:intergenic 40.0%
! TTGTAATATTGGGCTAGACC+TGG - chr3.3:77573594-77573613 None:intergenic 40.0%
!!! CGATTTTTTCAAGTTGCCAG+TGG + chr3.3:77574083-77574102 MS.gene06599:CDS 40.0%
!!! TTTTTTCAAGTTGCCAGTGG+AGG + chr3.3:77574086-77574105 MS.gene06599:CDS 40.0%
ATGTTACAACCAGTGGCATC+AGG - chr3.3:77573802-77573821 None:intergenic 45.0%
TTCGGGTCTGATGTAGCTTT+CGG - chr3.3:77573446-77573465 None:intergenic 45.0%
! AAGAGGAGGTGCATAATTGG+AGG + chr3.3:77574178-77574197 MS.gene06599:CDS 45.0%
! AGAGGAGGTGCATAATTGGA+GGG + chr3.3:77574179-77574198 MS.gene06599:CDS 45.0%
! CTTGTCCGTTGATAGATACG+TGG - chr3.3:77574321-77574340 None:intergenic 45.0%
! GACACCAAAGTGCTTTCTTC+CGG + chr3.3:77573400-77573419 MS.gene06599:CDS 45.0%
! GCCACTGGTTGTAACATAAG+TGG + chr3.3:77573805-77573824 MS.gene06599:intron 45.0%
!! GATTCCGGAAGAAAGCACTT+TGG - chr3.3:77573407-77573426 None:intergenic 45.0%
!!! CAGCTCCTATGGTTTTTTCC+AGG + chr3.3:77573573-77573592 MS.gene06599:CDS 45.0%
!! TTAATATATTGTTAATTAAT+TGG + chr3.3:77574000-77574019 MS.gene06599:intron 5.0%
ATGCGTAGTGTCTATGAGCC+AGG + chr3.3:77573831-77573850 MS.gene06599:intron 50.0%
GGAGGATTAGAAGGCCATTC+TGG - chr3.3:77574246-77574265 None:intergenic 50.0%
TCGGGTCTGATGTAGCTTTC+GGG - chr3.3:77573445-77573464 None:intergenic 50.0%
ACATGCAAGCCTGATGCCAC+TGG + chr3.3:77573790-77573809 MS.gene06599:intron 55.0%
GGTGAAGCATGCAGCTCCTA+TGG + chr3.3:77573562-77573581 MS.gene06599:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 77573397 77574357 77573397 ID=MS.gene06599
chr3.3 mRNA 77573397 77574357 77573397 ID=MS.gene06599.t1;Parent=MS.gene06599
chr3.3 exon 77573397 77573594 77573397 ID=MS.gene06599.t1.exon1;Parent=MS.gene06599.t1
chr3.3 CDS 77573397 77573594 77573397 ID=cds.MS.gene06599.t1;Parent=MS.gene06599.t1
chr3.3 exon 77574027 77574274 77574027 ID=MS.gene06599.t1.exon2;Parent=MS.gene06599.t1
chr3.3 CDS 77574027 77574274 77574027 ID=cds.MS.gene06599.t1;Parent=MS.gene06599.t1
chr3.3 exon 77574318 77574357 77574318 ID=MS.gene06599.t1.exon3;Parent=MS.gene06599.t1
chr3.3 CDS 77574318 77574357 77574318 ID=cds.MS.gene06599.t1;Parent=MS.gene06599.t1
Gene Sequence

>MS.gene06599

ATGGACACCAAAGTGCTTTCTTCCGGAATCCATTATTCAAAATTACCCGAAAGCTACATCAGACCCGAATCTGATAGACCATGTCTTTCTCAAGTCTCTCAGTTCGAAAATGTTCCTATCATTGATTTGGGTTCTCATAACAGAACACAGATTGTGCAGCAAATTGGTGAAGCATGCAGCTCCTATGGTTTTTTCCAGGTCTAGCCCAATATTACAATTTTTTGTTTATTCATTATTCAATAACCATAGATGAACAATGAAGATTATTTTAGATAGGCATAAGTCATAATTTTTGCTATTCATTGTAATTAGAATAGGATTAACTCCCTTCTCCAAAAAAAAAAAAAGAATAGGGTTAACTCCTCATTTCTACTAAATACAATTTGATTTGTCACATGCAAGCCTGATGCCACTGGTTGTAACATAAGTGGTTGATGCGTAGTGTCTATGAGCCAGGAACCCAGTTCAATTTTTAGTGATAGAATAAAATGTCATTTTGGTAATTAAGTGACATTGTGAATACAAAGCTAATTGAGTGATTGTGTAATGCTTAGTCACTGAATTAATTTTATATATTAAACACCGTAAGTTTTGGGAGACTAATTAATATATTGTTAATTAATTGGACAGGTAGTTAATCATGGGGTGCCTCTAGAAGAATTGAAAAAAACAGCAGAAGTGGCATACGATTTTTTCAAGTTGCCAGTGGAGGAGAAAATGAAGTTGTATTCAGACGATCCCACGAAGACAATGAGACTATCTACAAGTTTTAATGTGAACAAAGAGGAGGTGCATAATTGGAGGGACTACCTTAGACTTCATTGCTATCCTTTGGATAATTATGTGCCAGAATGGCCTTCTAATCCTCCTTCATTCAAGTAAGTCCACTCTCTTCATTTTTCTCCGTCTCTCTAATTTTAGCCACGTATCTATCAACGGACAAGTGTCACTGACTCACTGA
Protein sequence

>MS.gene06599.t1

MDTKVLSSGIHYSKLPESYIRPESDRPCLSQVSQFENVPIIDLGSHNRTQIVQQIGEACSSYGFFQVVNHGVPLEELKKTAEVAYDFFKLPVEEKMKLYSDDPTKTMRLSTSFNVNKEEVHNWRDYLRLHCYPLDNYVPEWPSNPPSFNHVSINGQVSLTH