AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057255

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057255.t1 MTR_3g092890 96.104 154 6 0 1 154 1 154 9.93e-108 311
MS.gene057255.t1 MTR_8g024120 57.246 138 55 2 15 148 18 155 1.76e-51 167
MS.gene057255.t1 MTR_1g070135 38.158 152 90 2 1 148 1 152 4.96e-33 118
MS.gene057255.t1 MTR_7g081710 41.844 141 77 2 13 148 23 163 1.16e-32 119
MS.gene057255.t1 MTR_1g070135 38.158 152 90 2 1 148 1 152 1.23e-32 119
MS.gene057255.t1 MTR_1g062110 38.217 157 87 4 4 152 29 183 1.76e-31 115
MS.gene057255.t1 MTR_1g062110 38.217 157 87 4 4 152 29 183 3.93e-31 115
MS.gene057255.t1 MTR_7g016090 36.601 153 95 2 1 151 3 155 7.41e-30 111
MS.gene057255.t1 MTR_7g016090 36.601 153 95 2 1 151 1 153 7.72e-30 111
MS.gene057255.t1 MTR_7g102820 38.776 147 81 2 14 151 37 183 1.19e-29 111
MS.gene057255.t1 MTR_1g070120 37.241 145 85 3 8 148 10 152 4.92e-29 109
MS.gene057255.t1 MTR_7g016080 36.129 155 95 3 1 151 1 155 2.26e-26 102
MS.gene057255.t1 MTR_1g048940 35.461 141 84 2 13 148 22 160 6.56e-26 101
MS.gene057255.t1 MTR_1g070085 33.750 160 102 2 4 159 3 162 4.88e-25 98.6
MS.gene057255.t1 MTR_1g070085 33.750 160 102 2 4 159 3 162 4.88e-25 98.6
MS.gene057255.t1 MTR_1g070080 36.735 147 88 3 4 145 3 149 1.19e-24 97.4
MS.gene057255.t1 MTR_5g082750 37.931 145 81 4 15 151 25 168 2.10e-24 97.1
MS.gene057255.t1 MTR_3g070860 37.013 154 87 5 6 151 18 169 1.11e-23 95.1
MS.gene057255.t1 MTR_5g032880 36.296 135 79 3 15 142 353 487 2.21e-20 87.0
MS.gene057255.t1 MTR_5g032880 37.879 132 77 3 15 144 15 143 3.21e-18 80.9
MS.gene057255.t1 MTR_7g068650 31.579 152 89 5 13 151 24 173 3.51e-20 85.9
MS.gene057255.t1 MTR_6g015950 31.169 154 103 2 1 151 1 154 4.99e-20 85.1
MS.gene057255.t1 MTR_3g043900 36.842 133 79 2 15 143 29 160 5.31e-20 85.1
MS.gene057255.t1 MTR_3g045230 36.134 119 72 1 15 129 29 147 7.87e-20 84.7
MS.gene057255.t1 MTR_4g015790 32.335 167 85 6 8 151 20 181 1.75e-19 84.0
MS.gene057255.t1 MTR_7g102720 38.298 94 58 0 36 129 35 128 1.81e-18 80.9
MS.gene057255.t1 MTR_5g032870 34.783 138 77 3 15 142 15 149 4.64e-18 79.7
MS.gene057255.t1 MTR_3g091350 34.677 124 68 4 37 152 58 176 5.27e-17 77.0
MS.gene057255.t1 MTR_5g055820 34.507 142 83 5 13 148 22 159 6.08e-17 76.6
MS.gene057255.t1 MTR_7g045650 30.282 142 94 4 14 151 35 175 7.89e-17 76.3
MS.gene057255.t1 MTR_5g065010 32.026 153 94 3 3 149 4 152 1.19e-16 75.9
MS.gene057255.t1 MTR_7g063730 32.353 136 87 4 14 145 39 173 2.30e-16 75.1
MS.gene057255.t1 MTR_3g058610 30.573 157 100 5 3 152 4 158 2.80e-16 74.7
MS.gene057255.t1 MTR_2g069300 39.785 93 51 1 38 125 54 146 3.22e-16 74.7
MS.gene057255.t1 MTR_3g091360 34.677 124 70 4 37 153 51 170 4.62e-16 74.3
MS.gene057255.t1 MTR_8g075890 29.861 144 94 3 15 152 16 158 9.68e-16 73.6
MS.gene057255.t1 MTR_7g062570 30.822 146 96 4 10 151 26 170 1.70e-15 72.8
MS.gene057255.t1 MTR_1g034140 30.405 148 80 4 18 148 7 148 2.55e-15 72.0
MS.gene057255.t1 MTR_7g062600 30.070 143 93 5 14 151 26 166 4.28e-15 71.6
MS.gene057255.t1 MTR_5g022020 27.564 156 104 5 3 151 4 157 4.36e-15 71.6
MS.gene057255.t1 MTR_7g062550 30.282 142 94 4 14 151 35 175 4.52e-15 71.6
MS.gene057255.t1 MTR_0032s0040 31.111 135 79 5 5 127 31 163 5.85e-15 71.2
MS.gene057255.t1 MTR_8g075830 29.167 144 95 3 15 152 16 158 9.69e-15 70.5
MS.gene057255.t1 MTR_5g048550 30.233 129 85 3 14 139 23 149 1.07e-14 70.5
MS.gene057255.t1 MTR_4g099380 32.061 131 85 2 5 133 141 269 1.09e-14 69.7
MS.gene057255.t1 MTR_5g048850 32.857 140 85 5 13 147 22 157 1.09e-14 69.7
MS.gene057255.t1 MTR_3g063180 30.380 158 98 6 5 154 13 166 1.15e-14 70.5
MS.gene057255.t1 MTR_5g059130 32.877 146 89 5 13 152 18 160 1.16e-14 70.1
MS.gene057255.t1 MTR_3g072810 33.333 141 89 4 14 151 16 154 1.36e-14 70.1
MS.gene057255.t1 MTR_4g087090 31.126 151 76 6 19 148 12 155 1.73e-14 69.7
MS.gene057255.t1 MTR_5g048850 31.387 137 89 3 13 146 22 156 1.79e-14 69.7
MS.gene057255.t1 MTR_1g034060 38.384 99 49 3 14 101 3 100 1.85e-14 69.7
MS.gene057255.t1 MTR_4g087100 32.716 162 82 7 13 151 2 159 1.86e-14 69.7
MS.gene057255.t1 MTR_1g034120 38.384 99 49 3 14 101 3 100 2.02e-14 69.7
MS.gene057255.t1 MTR_3g072820 28.369 141 98 3 15 152 18 158 2.48e-14 69.3
MS.gene057255.t1 MTR_1g032140 31.298 131 84 3 8 134 36 164 3.02e-14 69.3
MS.gene057255.t1 MTR_1g032220 29.577 142 94 3 5 142 40 179 3.09e-14 69.3
MS.gene057255.t1 MTR_5g055690 27.778 126 89 1 13 136 22 147 3.50e-14 68.6
MS.gene057255.t1 MTR_2g069190 34.409 93 56 1 38 125 68 160 3.93e-14 68.9
MS.gene057255.t1 MTR_4g093840 28.873 142 88 6 4 133 24 164 5.14e-14 68.6
MS.gene057255.t1 MTR_7g029655 33.835 133 67 5 8 127 34 158 5.72e-14 68.6
MS.gene057255.t1 MTR_1g032250 28.873 142 95 3 5 142 40 179 5.72e-14 68.6
MS.gene057255.t1 MTR_7g062580 28.671 143 95 5 14 151 30 170 6.14e-14 68.2
MS.gene057255.t1 MTR_5g055690 27.007 137 94 2 13 143 22 158 6.27e-14 68.2
MS.gene057255.t1 MTR_2g069210 32.673 101 63 1 38 133 64 164 6.28e-14 68.2
MS.gene057255.t1 MTR_2g068850 31.915 141 77 4 14 143 32 164 9.27e-14 66.6
MS.gene057255.t1 MTR_1g093120 31.159 138 84 3 5 133 32 167 9.36e-14 67.8
MS.gene057255.t1 MTR_8g028435 33.333 102 62 1 38 133 60 161 1.12e-13 67.8
MS.gene057255.t1 MTR_5g059140 29.012 162 103 6 1 152 1 160 1.21e-13 67.4
MS.gene057255.t1 MTR_8g009110 31.193 109 69 1 38 140 60 168 1.59e-13 67.0
MS.gene057255.t1 MTR_1g093140 28.369 141 89 3 5 133 33 173 2.10e-13 67.0
MS.gene057255.t1 MTR_8g009160 32.353 102 63 1 38 133 60 161 2.21e-13 66.6
MS.gene057255.t1 MTR_2g068840 31.915 141 77 4 14 143 32 164 2.35e-13 66.6
MS.gene057255.t1 MTR_2g069090 32.258 93 58 1 38 125 68 160 2.72e-13 66.6
MS.gene057255.t1 MTR_3g088565 35.593 118 58 6 38 148 3 109 3.25e-13 65.9
MS.gene057255.t1 MTR_5g048470 35.172 145 80 8 13 148 27 166 5.71e-13 65.5
MS.gene057255.t1 MTR_8g041600 35.714 98 56 1 37 127 67 164 6.57e-13 65.1
MS.gene057255.t1 MTR_2g072890 28.986 138 93 2 6 139 20 156 8.06e-13 65.1
MS.gene057255.t1 MTR_4g087120 33.613 119 66 4 18 127 7 121 8.11e-13 65.1
MS.gene057255.t1 MTR_8g041600 35.714 98 56 1 37 127 67 164 8.99e-13 65.1
MS.gene057255.t1 MTR_8g064200 33.858 127 74 5 13 133 27 149 9.74e-13 65.1
MS.gene057255.t1 MTR_8g064200 33.858 127 74 5 13 133 32 154 1.03e-12 64.7
MS.gene057255.t1 MTR_8g009150 31.193 109 69 1 38 140 60 168 1.15e-12 64.7
MS.gene057255.t1 MTR_8g009120 31.193 109 69 1 38 140 60 168 1.41e-12 64.3
MS.gene057255.t1 MTR_0009s0410 29.008 131 86 4 13 139 21 148 1.51e-12 62.4
MS.gene057255.t1 MTR_0009s0050 28.571 140 91 3 14 151 23 155 2.07e-12 63.9
MS.gene057255.t1 MTR_1g070185 32.117 137 85 4 5 135 37 171 2.11e-12 63.9
MS.gene057255.t1 MTR_0009s0050 28.571 140 91 3 14 151 23 155 2.23e-12 63.9
MS.gene057255.t1 MTR_2g033270 36.170 94 55 2 37 130 18 106 2.27e-12 63.5
MS.gene057255.t1 MTR_8g062600 31.373 102 65 1 37 133 66 167 2.40e-12 63.9
MS.gene057255.t1 MTR_5g055680 30.894 123 80 3 14 133 23 143 2.63e-12 63.5
MS.gene057255.t1 MTR_8g009090 34.043 94 56 1 38 125 60 153 3.15e-12 63.5
MS.gene057255.t1 MTR_2g068960 29.197 137 90 3 13 142 36 172 3.62e-12 63.2
MS.gene057255.t1 MTR_0328s0030 28.571 140 94 4 13 148 20 157 4.06e-12 63.2
MS.gene057255.t1 MTR_8g009170 29.358 109 71 1 38 140 60 168 4.17e-12 62.8
MS.gene057255.t1 MTR_4g100590 34.483 116 65 4 37 141 8 123 4.84e-12 62.8
MS.gene057255.t1 MTR_8g009170 29.358 109 71 1 38 140 60 168 5.24e-12 62.8
MS.gene057255.t1 MTR_8g008930 33.010 103 62 2 38 133 60 162 5.49e-12 62.8
MS.gene057255.t1 MTR_4g100600 43.548 62 35 0 37 98 8 69 6.87e-12 61.6
MS.gene057255.t1 MTR_4g024660 26.712 146 99 3 13 153 42 184 7.83e-12 62.4
MS.gene057255.t1 MTR_2g068950 31.532 111 71 2 37 142 62 172 9.07e-12 62.0
MS.gene057255.t1 MTR_4g100600 43.548 62 35 0 37 98 8 69 1.05e-11 62.0
MS.gene057255.t1 MTR_1g093110 29.496 139 87 3 5 133 33 170 1.21e-11 62.0
MS.gene057255.t1 MTR_5g055800 26.829 123 87 2 14 134 23 144 1.27e-11 61.6
MS.gene057255.t1 MTR_7g105070 28.777 139 91 4 1 133 22 158 1.38e-11 61.6
MS.gene057255.t1 MTR_2g072930 26.718 131 91 2 13 139 26 155 1.57e-11 59.7
MS.gene057255.t1 MTR_0009s0480 28.000 125 85 3 13 134 21 143 1.58e-11 60.8
MS.gene057255.t1 MTR_5g011250 27.673 159 98 7 8 152 14 169 1.74e-11 61.2
MS.gene057255.t1 MTR_2g437380 26.429 140 90 6 15 145 28 163 2.50e-11 60.8
MS.gene057255.t1 MTR_0009s0480 28.000 125 85 3 13 134 21 143 2.57e-11 60.8
MS.gene057255.t1 MTR_8g041620 33.673 98 58 1 37 127 67 164 2.58e-11 60.8
MS.gene057255.t1 MTR_5g048740 25.850 147 98 4 2 139 3 147 2.70e-11 60.8
MS.gene057255.t1 MTR_4g087140 35.789 95 51 3 17 102 6 99 2.80e-11 60.8
MS.gene057255.t1 MTR_4g087130 32.203 118 70 3 18 126 7 123 4.24e-11 60.1
MS.gene057255.t1 MTR_5g055900 31.522 92 59 2 13 101 22 112 6.40e-11 59.7
MS.gene057255.t1 MTR_2g102570 29.323 133 87 4 14 144 27 154 6.53e-11 59.7
MS.gene057255.t1 MTR_4g119410 28.358 134 89 3 5 133 36 167 8.66e-11 59.3
MS.gene057255.t1 MTR_4g116010 31.858 113 75 2 38 149 50 161 9.64e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057255.t1 AT5G24530 60.265 151 60 0 1 151 1 151 1.41e-65 204
MS.gene057255.t1 AT4G10500 50.633 158 71 3 6 159 14 168 2.24e-49 162
MS.gene057255.t1 AT4G10490 52.113 142 64 2 15 152 18 159 5.32e-47 156
MS.gene057255.t1 AT2G36690 41.379 145 76 2 13 148 30 174 1.35e-32 119
MS.gene057255.t1 AT2G36690 41.379 145 76 2 13 148 30 174 1.35e-32 119
MS.gene057255.t1 AT3G60290 41.429 140 76 3 15 148 27 166 8.11e-30 111
MS.gene057255.t1 AT2G44800 37.589 141 82 2 14 148 26 166 5.52e-29 109
MS.gene057255.t1 AT5G07480 35.971 139 81 2 15 148 24 159 1.34e-24 97.8
MS.gene057255.t1 AT3G13610 39.316 117 69 2 15 129 38 154 6.22e-22 90.5
MS.gene057255.t1 AT5G05600 37.162 148 82 5 13 151 36 181 1.47e-20 85.9
MS.gene057255.t1 AT5G12270 32.867 143 94 2 3 143 21 163 4.02e-20 85.5
MS.gene057255.t1 AT5G05600 37.162 148 82 5 13 151 36 181 4.08e-20 85.9
MS.gene057255.t1 AT3G11180 35.570 149 85 5 13 152 65 211 2.01e-19 84.0
MS.gene057255.t1 AT3G11180 37.500 144 76 5 13 144 65 206 2.15e-19 84.0
MS.gene057255.t1 AT1G55290 34.351 131 83 2 15 142 37 167 3.01e-19 83.2
MS.gene057255.t1 AT3G51240 30.822 146 94 3 13 152 13 157 1.67e-17 78.6
MS.gene057255.t1 AT5G08640 28.289 152 104 4 5 152 9 159 4.98e-16 73.9
MS.gene057255.t1 AT5G08640 28.289 152 104 4 5 152 9 159 4.98e-16 73.9
MS.gene057255.t1 AT1G06620 35.294 102 61 1 37 133 61 162 5.71e-16 73.6
MS.gene057255.t1 AT2G38240 29.078 141 95 4 15 151 23 162 1.06e-15 73.2
MS.gene057255.t1 AT1G06620 35.294 102 61 1 37 133 61 162 1.13e-15 73.2
MS.gene057255.t1 AT3G12900 32.394 142 90 3 5 142 25 164 1.38e-15 73.2
MS.gene057255.t1 AT3G55970 30.345 145 87 5 13 145 23 165 2.14e-15 72.0
MS.gene057255.t1 AT3G55970 30.769 143 85 5 15 145 25 165 3.88e-15 71.6
MS.gene057255.t1 AT1G06640 31.884 138 80 4 5 132 29 162 1.01e-14 70.1
MS.gene057255.t1 AT1G06640 31.884 138 80 4 5 132 29 162 1.14e-14 70.1
MS.gene057255.t1 AT1G06640 31.884 138 80 4 5 132 29 162 1.37e-14 70.1
MS.gene057255.t1 AT3G19000 29.518 166 84 6 13 151 2 161 2.64e-14 68.9
MS.gene057255.t1 AT3G19000 29.518 166 84 6 13 151 2 161 6.43e-14 68.2
MS.gene057255.t1 AT1G06650 28.058 139 88 3 5 133 29 165 9.51e-14 67.8
MS.gene057255.t1 AT1G06650 29.008 131 81 3 5 125 29 157 1.18e-13 67.8
MS.gene057255.t1 AT2G30830 31.373 102 64 1 38 133 54 155 1.44e-13 67.4
MS.gene057255.t1 AT4G16330 30.000 120 77 4 37 151 66 183 2.00e-13 67.0
MS.gene057255.t1 AT5G43440 34.259 108 67 1 37 140 61 168 2.03e-13 66.6
MS.gene057255.t1 AT5G43440 34.259 108 67 1 37 140 61 168 2.77e-13 66.6
MS.gene057255.t1 AT5G43935 29.600 125 79 4 27 149 12 129 3.09e-13 65.9
MS.gene057255.t1 AT1G06645 30.534 131 79 3 5 125 42 170 3.77e-13 66.2
MS.gene057255.t1 AT5G63600 30.935 139 81 4 23 152 11 143 3.99e-13 65.9
MS.gene057255.t1 AT1G77330 36.036 111 61 4 38 144 3 107 4.05e-13 65.9
MS.gene057255.t1 AT5G63600 30.935 139 81 4 23 152 11 143 4.33e-13 65.9
MS.gene057255.t1 AT5G63595 28.000 125 82 3 30 152 17 135 7.98e-13 64.7
MS.gene057255.t1 AT3G19010 42.254 71 37 1 36 102 26 96 8.28e-13 65.1
MS.gene057255.t1 AT3G19010 42.254 71 37 1 36 102 26 96 1.09e-12 64.7
MS.gene057255.t1 AT3G19010 42.254 71 37 1 36 102 26 96 1.09e-12 64.7
MS.gene057255.t1 AT3G19010 42.254 71 37 1 36 102 26 96 1.36e-12 64.3
MS.gene057255.t1 AT1G04350 31.206 141 87 6 2 133 17 156 1.45e-12 64.3
MS.gene057255.t1 AT3G21420 30.000 150 89 5 13 149 26 172 1.59e-12 64.3
MS.gene057255.t1 AT4G25300 30.709 127 82 3 13 134 26 151 1.81e-12 64.3
MS.gene057255.t1 AT5G63590 29.661 118 81 2 37 152 13 130 1.89e-12 63.9
MS.gene057255.t1 AT3G61400 32.710 107 62 1 37 133 59 165 2.05e-12 63.5
MS.gene057255.t1 AT3G61400 32.710 107 62 1 37 133 59 165 2.43e-12 63.9
MS.gene057255.t1 AT1G80340 27.737 137 87 3 12 144 24 152 3.32e-12 63.5
MS.gene057255.t1 AT5G54000 30.769 156 92 6 4 148 11 161 5.59e-12 62.8
MS.gene057255.t1 AT1G15550 30.657 137 79 4 14 142 29 157 6.36e-12 62.8
MS.gene057255.t1 AT5G63580 29.752 121 75 4 36 151 17 132 9.02e-12 61.6
MS.gene057255.t1 AT5G63580 29.752 121 75 4 36 151 17 132 9.52e-12 61.6
MS.gene057255.t1 AT1G17010 30.469 128 82 3 13 134 27 153 1.38e-11 61.6
MS.gene057255.t1 AT1G03400 29.897 97 65 1 37 133 55 148 2.34e-11 60.8
MS.gene057255.t1 AT1G03410 29.710 138 86 5 5 133 23 158 2.96e-11 60.8
MS.gene057255.t1 AT1G03400 29.897 97 65 1 37 133 55 148 3.52e-11 60.5
MS.gene057255.t1 AT1G03410 29.710 138 86 5 5 133 60 195 4.55e-11 60.5
MS.gene057255.t1 AT1G17020 27.972 143 96 4 13 149 27 168 6.50e-11 59.7
MS.gene057255.t1 AT2G30840 30.097 103 65 1 38 133 57 159 7.64e-11 59.7
MS.gene057255.t1 AT2G30840 30.097 103 65 1 38 133 57 159 8.17e-11 59.3
MS.gene057255.t1 AT5G51810 31.092 119 78 4 13 130 40 155 9.63e-11 58.9

Find 30 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGTTCCTATCATTGATT+TGG 0.233266 3.2:+78152055 MS.gene057255:CDS
TTCGGGTCTGATGTAGCTTT+CGG 0.284448 3.2:-78151994 None:intergenic
TCGGGTCTGATGTAGCTTTC+GGG 0.320790 3.2:-78151993 None:intergenic
GGAGGATTAGAAGGCCATTC+TGG 0.349419 3.2:-78152962 None:intergenic
TAGACTTCATTGCTATCCTT+TGG 0.360775 3.2:+78152928 MS.gene057255:CDS
GACACCAAAGTGCTTTCTTC+CGG 0.368362 3.2:+78151951 MS.gene057255:CDS
TTGAATGAAGGAGGATTAGA+AGG 0.374736 3.2:-78152971 None:intergenic
AATGTTCCTATCATTGATTT+GGG 0.394239 3.2:+78152056 MS.gene057255:CDS
ACACAGATTGTGCAGCAAAT+TGG 0.405459 3.2:+78152092 MS.gene057255:CDS
AAGACATGGTCTATCAGATT+CGG 0.410372 3.2:-78152012 None:intergenic
AAGGATAGCAATGAAGTCTA+AGG 0.423736 3.2:-78152925 None:intergenic
ACAAAGAGGAGGTGCACAAT+TGG 0.436674 3.2:+78152894 MS.gene057255:CDS
ATTGGACAGGTAGTTAATCA+TGG 0.442304 3.2:+78152737 MS.gene057255:intron
GGTGAAGCATGCAGCTCCTA+TGG 0.451140 3.2:+78152113 MS.gene057255:CDS
AGACATGGTCTATCAGATTC+GGG 0.453451 3.2:-78152011 None:intergenic
TGGATAATTATGTGCCAGAA+TGG 0.460872 3.2:+78152948 MS.gene057255:CDS
TGAGAACCCAAATCAATGAT+AGG 0.480759 3.2:-78152062 None:intergenic
TTGGACAGGTAGTTAATCAT+GGG 0.500876 3.2:+78152738 MS.gene057255:intron
GATAGTCTCATTGTCTTCGT+GGG 0.526905 3.2:-78152854 None:intergenic
AGATAGTCTCATTGTCTTCG+TGG 0.538477 3.2:-78152855 None:intergenic
AAGAGGAGGTGCACAATTGG+AGG 0.546447 3.2:+78152897 MS.gene057255:CDS
GAGTGGACTTACTTGAATGA+AGG 0.551937 3.2:-78152983 None:intergenic
TGGACTTACTTGAATGAAGG+AGG 0.584641 3.2:-78152980 None:intergenic
GATTCCGGAAGAAAGCACTT+TGG 0.619711 3.2:-78151955 None:intergenic
TTCAGCCACGTATCTATCAA+CGG 0.629093 3.2:+78153032 MS.gene057255:intron
AGAGGAGGTGCACAATTGGA+GGG 0.630346 3.2:+78152898 MS.gene057255:CDS
TCTGGCACATAATTATCCAA+AGG 0.648305 3.2:-78152944 None:intergenic
TCAGAGACTTGAGAAAGACA+TGG 0.682588 3.2:-78152026 None:intergenic
CTTGTCCGTTGATAGATACG+TGG 0.692724 3.2:-78153037 None:intergenic
TGGACAGGTAGTTAATCATG+GGG 0.699574 3.2:+78152739 MS.gene057255:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAAAAAATTGTAATATT+GGG - chr3.2:78152155-78152174 None:intergenic 10.0%
!! TAAACAAAAAATTGTAATAT+TGG - chr3.2:78152156-78152175 None:intergenic 10.0%
!! TCCAAAAAAAAAAAAAGAAT+AGG + chr3.2:78152279-78152298 MS.gene057255:intron 15.0%
!!! TCCTATTCTTTTTTTTTTTT+TGG - chr3.2:78152283-78152302 None:intergenic 15.0%
!! AATTGTATTTAGTAGAAATG+AGG - chr3.2:78152312-78152331 None:intergenic 20.0%
!! ATATTGTTAATTAATTGGAC+AGG + chr3.2:78152724-78152743 MS.gene057255:intron 20.0%
!! ATGTATGTAGTCTTTATTAT+AGG - chr3.2:78152461-78152480 None:intergenic 20.0%
!! CTATTCATTGTAATTAGAAT+AGG + chr3.2:78152243-78152262 MS.gene057255:intron 20.0%
!! TAAGCAAAAATTCAATTTCT+AGG - chr3.2:78152499-78152518 None:intergenic 20.0%
!!! CAATGAAGATTATTTTAGAT+AGG + chr3.2:78152202-78152221 MS.gene057255:intron 20.0%
!!! CTTTTTTTTTTTTTGGAGAA+GGG - chr3.2:78152276-78152295 None:intergenic 20.0%
!!! GATAGAATAAAATGTCATTT+TGG + chr3.2:78152593-78152612 MS.gene057255:intron 20.0%
!!! TAATTTTGAATAATGGATTC+CGG - chr3.2:78151973-78151992 None:intergenic 20.0%
!!! TCTTTTTTTTTTTTTGGAGA+AGG - chr3.2:78152277-78152296 None:intergenic 20.0%
! CTATCACTAAAAATTGAACT+GGG - chr3.2:78152578-78152597 None:intergenic 25.0%
! TAATCTTCATTGTTCATCTA+TGG - chr3.2:78152194-78152213 None:intergenic 25.0%
! TATTGAATGAACCTAGAAAA+TGG - chr3.2:78152424-78152443 None:intergenic 25.0%
! TCTATCACTAAAAATTGAAC+TGG - chr3.2:78152579-78152598 None:intergenic 25.0%
!! AAATGTTCCTATCATTGATT+TGG + chr3.2:78152055-78152074 MS.gene057255:CDS 25.0%
!! ATATTAAACACCGTAAGTTT+TGG + chr3.2:78152688-78152707 MS.gene057255:intron 25.0%
!! TAAGCAAAAATCCATTTTCT+AGG + chr3.2:78152410-78152429 MS.gene057255:intron 25.0%
!! TATTAAACACCGTAAGTTTT+GGG + chr3.2:78152689-78152708 MS.gene057255:intron 25.0%
!! TTTTTGCTTATATTAGTGAC+CGG + chr3.2:78152509-78152528 MS.gene057255:intron 25.0%
!! TTTTTGCTTATATTAGTGAC+CGG - chr3.2:78152528-78152509 None:intergenic 25.0%
!!! AAGTTTTAATGTGAACAAAG+AGG + chr3.2:78152880-78152899 MS.gene057255:CDS 25.0%
!!! AATGTTCCTATCATTGATTT+GGG + chr3.2:78152056-78152075 MS.gene057255:CDS 25.0%
!!! GTTTTTTTCAATTCTTCTAG+AGG - chr3.2:78152767-78152786 None:intergenic 25.0%
!!! TTTCGGGTAATTTTGAATAA+TGG - chr3.2:78151980-78151999 None:intergenic 25.0%
ATTGAAAAAAACAGCAGAAG+TGG + chr3.2:78152775-78152794 MS.gene057255:CDS 30.0%
! AATTAGTCTCCCAAAACTTA+CGG - chr3.2:78152701-78152720 None:intergenic 30.0%
! TTTTAATGTGAACAAAGAGG+AGG + chr3.2:78152883-78152902 MS.gene057255:CDS 30.0%
AAGACATGGTCTATCAGATT+CGG - chr3.2:78152015-78152034 None:intergenic 35.0%
AAGGATAGCAATGAAGTCTA+AGG - chr3.2:78152928-78152947 None:intergenic 35.0%
ATTGGACAGGTAGTTAATCA+TGG + chr3.2:78152737-78152756 MS.gene057255:intron 35.0%
TAGACTTCATTGCTATCCTT+TGG + chr3.2:78152928-78152947 MS.gene057255:CDS 35.0%
TCTGGCACATAATTATCCAA+AGG - chr3.2:78152947-78152966 None:intergenic 35.0%
TGAGAACCCAAATCAATGAT+AGG - chr3.2:78152065-78152084 None:intergenic 35.0%
TGGATAATTATGTGCCAGAA+TGG + chr3.2:78152948-78152967 MS.gene057255:CDS 35.0%
TTGAATGAAGGAGGATTAGA+AGG - chr3.2:78152974-78152993 None:intergenic 35.0%
TTGGACAGGTAGTTAATCAT+GGG + chr3.2:78152738-78152757 MS.gene057255:intron 35.0%
! AACTTCATTTTCTCATCCAC+TGG - chr3.2:78152821-78152840 None:intergenic 35.0%
!!! TGATTTTTTCAAGTTGCCAG+TGG + chr3.2:78152802-78152821 MS.gene057255:CDS 35.0%
ACACAGATTGTGCAGCAAAT+TGG + chr3.2:78152092-78152111 MS.gene057255:CDS 40.0%
ACGGAGAAAAATGAAGAGAG+TGG - chr3.2:78153003-78153022 None:intergenic 40.0%
AGACATGGTCTATCAGATTC+GGG - chr3.2:78152014-78152033 None:intergenic 40.0%
AGATAGTCTCATTGTCTTCG+TGG - chr3.2:78152858-78152877 None:intergenic 40.0%
CTAGACCTGGAAAAAACCAT+AGG - chr3.2:78152132-78152151 None:intergenic 40.0%
GATAGTCTCATTGTCTTCGT+GGG - chr3.2:78152857-78152876 None:intergenic 40.0%
TCAGAGACTTGAGAAAGACA+TGG - chr3.2:78152029-78152048 None:intergenic 40.0%
TGCTTATATTAGTGACCGGA+GGG - chr3.2:78152532-78152513 None:intergenic 40.0%
TGGACAGGTAGTTAATCATG+GGG + chr3.2:78152739-78152758 MS.gene057255:intron 40.0%
TGGACTTACTTGAATGAAGG+AGG - chr3.2:78152983-78153002 None:intergenic 40.0%
TTCAGCCACGTATCTATCAA+CGG + chr3.2:78153032-78153051 MS.gene057255:intron 40.0%
TTGCTTATATTAGTGACCGG+AGG + chr3.2:78152512-78152531 MS.gene057255:intron 40.0%
TTGCTTATATTAGTGACCGG+AGG - chr3.2:78152531-78152512 None:intergenic 40.0%
! GAGTGGACTTACTTGAATGA+AGG - chr3.2:78152986-78153005 None:intergenic 40.0%
! TTGTAATATTGGGCTAGACC+TGG - chr3.2:78152145-78152164 None:intergenic 40.0%
!!! ATTTTAGAACCAGTGGCATC+AGG - chr3.2:78152353-78152372 None:intergenic 40.0%
AGGGTGTATGTTACAACCAG+TGG - chr3.2:78152377-78152396 None:intergenic 45.0%
CATCAACCACTTACATCCTC+CGG - chr3.2:78152531-78152550 None:intergenic 45.0%
CGTGGCTGAAATTAGAGAGA+CGG - chr3.2:78153022-78153041 None:intergenic 45.0%
GCGTATAGTGTCTATGAGTC+AGG + chr3.2:78152550-78152569 MS.gene057255:intron 45.0%
TGGTTGTAACATACACCCTC+CGG + chr3.2:78152378-78152397 MS.gene057255:intron 45.0%
TTCGGGTCTGATGTAGCTTT+CGG - chr3.2:78151997-78152016 None:intergenic 45.0%
! ACAAAGAGGAGGTGCACAAT+TGG + chr3.2:78152894-78152913 MS.gene057255:CDS 45.0%
! CTTGTCCGTTGATAGATACG+TGG - chr3.2:78153040-78153059 None:intergenic 45.0%
! GACACCAAAGTGCTTTCTTC+CGG + chr3.2:78151951-78151970 MS.gene057255:CDS 45.0%
!! CACTGGTTCTAAAATGCCAC+TGG + chr3.2:78152358-78152377 MS.gene057255:intron 45.0%
!! GATTCCGGAAGAAAGCACTT+TGG - chr3.2:78151958-78151977 None:intergenic 45.0%
!!! CAGCTCCTATGGTTTTTTCC+AGG + chr3.2:78152124-78152143 MS.gene057255:CDS 45.0%
!!! CAGTGGCATTTTAGAACCAG+TGG - chr3.2:78152360-78152379 None:intergenic 45.0%
!! TTAATATATTGTTAATTAAT+TGG + chr3.2:78152719-78152738 MS.gene057255:intron 5.0%
GGAGGATTAGAAGGCCATTC+TGG - chr3.2:78152965-78152984 None:intergenic 50.0%
TAGTGACCGGAGGATGTAAG+TGG + chr3.2:78152522-78152541 MS.gene057255:intron 50.0%
TCGGGTCTGATGTAGCTTTC+GGG - chr3.2:78151996-78152015 None:intergenic 50.0%
! AAGAGGAGGTGCACAATTGG+AGG + chr3.2:78152897-78152916 MS.gene057255:CDS 50.0%
! AGAGGAGGTGCACAATTGGA+GGG + chr3.2:78152898-78152917 MS.gene057255:CDS 50.0%
ACATGCAAGCCTGATGCCAC+TGG + chr3.2:78152341-78152360 MS.gene057255:intron 55.0%
GGTGAAGCATGCAGCTCCTA+TGG + chr3.2:78152113-78152132 MS.gene057255:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 78151948 78153076 78151948 ID=MS.gene057255
chr3.2 mRNA 78151948 78153076 78151948 ID=MS.gene057255.t1;Parent=MS.gene057255
chr3.2 exon 78151948 78152145 78151948 ID=MS.gene057255.t1.exon1;Parent=MS.gene057255.t1
chr3.2 CDS 78151948 78152145 78151948 ID=cds.MS.gene057255.t1;Parent=MS.gene057255.t1
chr3.2 exon 78152746 78152993 78152746 ID=MS.gene057255.t1.exon2;Parent=MS.gene057255.t1
chr3.2 CDS 78152746 78152993 78152746 ID=cds.MS.gene057255.t1;Parent=MS.gene057255.t1
chr3.2 exon 78153037 78153076 78153037 ID=MS.gene057255.t1.exon3;Parent=MS.gene057255.t1
chr3.2 CDS 78153037 78153076 78153037 ID=cds.MS.gene057255.t1;Parent=MS.gene057255.t1
Gene Sequence

>MS.gene057255

ATGGACACCAAAGTGCTTTCTTCCGGAATCCATTATTCAAAATTACCCGAAAGCTACATCAGACCCGAATCTGATAGACCATGTCTTTCTCAAGTCTCTGAGTTCGAAAATGTTCCTATCATTGATTTGGGTTCTCACAACAGAACACAGATTGTGCAGCAAATTGGTGAAGCATGCAGCTCCTATGGTTTTTTCCAGGTCTAGCCCAATATTACAATTTTTTGTTTATTCATTATTCAATAACCATAGATGAACAATGAAGATTATTTTAGATAGGCATAAGTCATAATTTTTGCTATTCATTGTAATTAGAATAGGATTAACTCCCTTCTCCAAAAAAAAAAAAAGAATAGGATTAACTCCTCATTTCTACTAAATACAATTTGATTTGTCACATGCAAGCCTGATGCCACTGGTTCTAAAATGCCACTGGTTGTAACATACACCCTCCGGTCACTAATATAAGCAAAAATCCATTTTCTAGGTTCATTCAATAAATGATGTATGTAGCCTATAATAAAGACTACATACATCATTAATTGAATGAACCTAGAAATTGAATTTTTGCTTATATTAGTGACCGGAGGATGTAAGTGGTTGATGCGTATAGTGTCTATGAGTCAGGAACCCAGTTCAATTTTTAGTGATAGAATAAAATGTCATTTTGGTAATTAAGTGACATTGTGAATACAAAGCTAATTGAGTGATTGTGTAATGCTTAGTCACTGAATTAATTTTATATATTAAACACCGTAAGTTTTGGGAGACTAATTAATATATTGTTAATTAATTGGACAGGTAGTTAATCATGGGGTGCCTCTAGAAGAATTGAAAAAAACAGCAGAAGTGGCATATGATTTTTTCAAGTTGCCAGTGGATGAGAAAATGAAGTTGTATTCAGACGATCCCACGAAGACAATGAGACTATCTACAAGTTTTAATGTGAACAAAGAGGAGGTGCACAATTGGAGGGACTACCTTAGACTTCATTGCTATCCTTTGGATAATTATGTGCCAGAATGGCCTTCTAATCCTCCTTCATTCAAGTAAGTCCACTCTCTTCATTTTTCTCCGTCTCTCTAATTTCAGCCACGTATCTATCAACGGACAAGTGTCACTGACTCACTGA
Protein sequence

>MS.gene057255.t1

MDTKVLSSGIHYSKLPESYIRPESDRPCLSQVSEFENVPIIDLGSHNRTQIVQQIGEACSSYGFFQVVNHGVPLEELKKTAEVAYDFFKLPVDEKMKLYSDDPTKTMRLSTSFNVNKEEVHNWRDYLRLHCYPLDNYVPEWPSNPPSFNHVSINGQVSLTH