Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066075.t1 | XP_013470234.1 | 100 | 71 | 0 | 0 | 6 | 76 | 324 | 394 | 1.20E-31 | 145.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066075.t1 | A0A072VR59 | 100.0 | 71 | 0 | 0 | 6 | 76 | 324 | 394 | 8.9e-32 | 145.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05216 | MS.gene066075 | 0.808207 | 3.47E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene066075 | 0.804758 | 1.85E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066075.t1 | MTR_1g111550 | 100.000 | 71 | 0 | 0 | 6 | 76 | 324 | 394 | 3.07e-43 | 147 |
| MS.gene066075.t1 | MTR_1g111510 | 84.507 | 71 | 11 | 0 | 6 | 76 | 326 | 396 | 1.46e-35 | 125 |
| MS.gene066075.t1 | MTR_1g111540 | 84.507 | 71 | 11 | 0 | 6 | 76 | 326 | 396 | 1.46e-35 | 125 |
| MS.gene066075.t1 | MTR_1g111530 | 81.690 | 71 | 13 | 0 | 6 | 76 | 322 | 392 | 4.05e-35 | 124 |
| MS.gene066075.t1 | MTR_7g102880 | 81.690 | 71 | 13 | 0 | 6 | 76 | 338 | 408 | 1.57e-33 | 120 |
| MS.gene066075.t1 | MTR_1g050550 | 65.217 | 69 | 24 | 0 | 8 | 76 | 335 | 403 | 3.69e-26 | 99.8 |
| MS.gene066075.t1 | MTR_1g059080 | 62.319 | 69 | 26 | 0 | 8 | 76 | 326 | 394 | 4.48e-25 | 96.7 |
| MS.gene066075.t1 | MTR_7g029015 | 55.882 | 68 | 28 | 1 | 9 | 76 | 342 | 407 | 3.71e-18 | 77.0 |
| MS.gene066075.t1 | MTR_4g073690 | 48.529 | 68 | 33 | 1 | 9 | 76 | 317 | 382 | 1.17e-14 | 67.0 |
| MS.gene066075.t1 | MTR_4g073690 | 48.529 | 68 | 33 | 1 | 9 | 76 | 317 | 382 | 1.40e-14 | 67.0 |
| MS.gene066075.t1 | MTR_5g022380 | 44.118 | 68 | 36 | 1 | 9 | 76 | 195 | 260 | 4.60e-13 | 62.4 |
| MS.gene066075.t1 | MTR_5g022380 | 44.118 | 68 | 36 | 1 | 9 | 76 | 305 | 370 | 4.69e-13 | 62.4 |
| MS.gene066075.t1 | MTR_5g090570 | 40.299 | 67 | 40 | 0 | 10 | 76 | 308 | 374 | 9.11e-13 | 61.6 |
| MS.gene066075.t1 | MTR_3g099150 | 44.118 | 68 | 36 | 1 | 9 | 76 | 315 | 380 | 1.24e-12 | 61.2 |
| MS.gene066075.t1 | MTR_2g100400 | 43.750 | 64 | 36 | 0 | 13 | 76 | 317 | 380 | 3.09e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066075.t1 | AT2G39210 | 81.690 | 71 | 13 | 0 | 6 | 76 | 325 | 395 | 1.16e-35 | 126 |
| MS.gene066075.t1 | AT2G28120 | 70.423 | 71 | 21 | 0 | 6 | 76 | 316 | 386 | 2.80e-30 | 111 |
| MS.gene066075.t1 | AT5G50520 | 51.220 | 82 | 32 | 2 | 1 | 76 | 295 | 374 | 3.17e-16 | 71.6 |
| MS.gene066075.t1 | AT5G50630 | 51.220 | 82 | 32 | 2 | 1 | 76 | 295 | 374 | 3.17e-16 | 71.6 |
| MS.gene066075.t1 | AT3G01930 | 55.882 | 68 | 28 | 1 | 9 | 76 | 352 | 417 | 4.12e-16 | 71.2 |
| MS.gene066075.t1 | AT3G01930 | 55.882 | 68 | 28 | 1 | 9 | 76 | 239 | 304 | 4.26e-16 | 71.2 |
| MS.gene066075.t1 | AT3G01930 | 55.882 | 68 | 28 | 1 | 9 | 76 | 352 | 417 | 4.48e-16 | 71.2 |
| MS.gene066075.t1 | AT2G16660 | 48.529 | 68 | 33 | 1 | 9 | 76 | 313 | 378 | 1.00e-15 | 70.1 |
| MS.gene066075.t1 | AT2G16660 | 48.529 | 68 | 33 | 1 | 9 | 76 | 313 | 378 | 1.04e-15 | 70.1 |
| MS.gene066075.t1 | AT5G14120 | 54.412 | 68 | 29 | 1 | 9 | 76 | 347 | 412 | 3.00e-15 | 68.9 |
| MS.gene066075.t1 | AT4G34950 | 47.059 | 68 | 34 | 1 | 9 | 76 | 334 | 399 | 3.68e-15 | 68.6 |
| MS.gene066075.t1 | AT1G74780 | 47.222 | 72 | 36 | 1 | 7 | 76 | 303 | 374 | 6.15e-14 | 65.1 |
| MS.gene066075.t1 | AT2G34355 | 44.615 | 65 | 36 | 0 | 12 | 76 | 151 | 215 | 2.30e-13 | 63.5 |
| MS.gene066075.t1 | AT2G34355 | 44.615 | 65 | 36 | 0 | 12 | 76 | 301 | 365 | 2.79e-13 | 63.2 |
| MS.gene066075.t1 | AT1G18940 | 38.806 | 67 | 41 | 0 | 10 | 76 | 301 | 367 | 6.71e-13 | 62.0 |
| MS.gene066075.t1 | AT2G34350 | 43.750 | 64 | 36 | 0 | 13 | 76 | 304 | 367 | 3.20e-12 | 60.1 |
| MS.gene066075.t1 | AT2G34350 | 43.750 | 64 | 36 | 0 | 13 | 76 | 304 | 367 | 3.20e-12 | 60.1 |
Find 14 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTCATTAGTGAGTATT+TGG | 0.206900 | 1.4:-85486754 | MS.gene066075:CDS |
| ATTGATAATTTAGGTCAAAT+TGG | 0.249106 | 1.4:-85486815 | MS.gene066075:CDS |
| TGTAAAATCTTCACCTTGTT+CGG | 0.272593 | 1.4:+85486919 | None:intergenic |
| TTAACAGCAATTGATAATTT+AGG | 0.275096 | 1.4:-85486824 | MS.gene066075:CDS |
| ATGTTATTGCTATTCTTTGC+TGG | 0.355922 | 1.4:-85486869 | MS.gene066075:CDS |
| GTAAAATCTTCACCTTGTTC+GGG | 0.366683 | 1.4:+85486920 | None:intergenic |
| ATAACATATCTATGCTGAAA+AGG | 0.416968 | 1.4:+85486885 | None:intergenic |
| GGTCAAATTGGAACCTCACT+TGG | 0.457053 | 1.4:-85486803 | MS.gene066075:CDS |
| TAACATATCTATGCTGAAAA+GGG | 0.471235 | 1.4:+85486886 | None:intergenic |
| TTTGCTGGTACATGTGGTGT+TGG | 0.472904 | 1.4:-85486854 | MS.gene066075:CDS |
| GCTGGTACATGTGGTGTTGG+TGG | 0.500330 | 1.4:-85486851 | MS.gene066075:CDS |
| CTATTCTTTGCTGGTACATG+TGG | 0.516615 | 1.4:-85486860 | MS.gene066075:CDS |
| GTGAGTATTTGGAATTACCT+AGG | 0.563575 | 1.4:-85486743 | MS.gene066075:CDS |
| AAATCTTCACCTTGTTCGGG+TGG | 0.607960 | 1.4:+85486923 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGATAATTTAGGTCAAAT+TGG | - | chr1.4:85486868-85486887 | MS.gene066075:CDS | 20.0% |
| !!! | TTAACAGCAATTGATAATTT+AGG | - | chr1.4:85486859-85486878 | MS.gene066075:CDS | 20.0% |
| ! | ATAACATATCTATGCTGAAA+AGG | + | chr1.4:85486801-85486820 | None:intergenic | 25.0% |
| ! | TAACATATCTATGCTGAAAA+GGG | + | chr1.4:85486800-85486819 | None:intergenic | 25.0% |
| ! | TTGTTTCATTAGTGAGTATT+TGG | - | chr1.4:85486929-85486948 | MS.gene066075:CDS | 25.0% |
| TGTAAAATCTTCACCTTGTT+CGG | + | chr1.4:85486767-85486786 | None:intergenic | 30.0% | |
| ! | ATGTTATTGCTATTCTTTGC+TGG | - | chr1.4:85486814-85486833 | MS.gene066075:CDS | 30.0% |
| !! | AATGTGCTTATGCTTGTTTT+TGG | + | chr1.4:85486910-85486929 | None:intergenic | 30.0% |
| !! | ATGTGCTTATGCTTGTTTTT+GGG | + | chr1.4:85486909-85486928 | None:intergenic | 30.0% |
| GTAAAATCTTCACCTTGTTC+GGG | + | chr1.4:85486766-85486785 | None:intergenic | 35.0% | |
| ! | GTGAGTATTTGGAATTACCT+AGG | - | chr1.4:85486940-85486959 | MS.gene066075:CDS | 35.0% |
| CTATTCTTTGCTGGTACATG+TGG | - | chr1.4:85486823-85486842 | MS.gene066075:CDS | 40.0% | |
| !!! | TATTTTAGTCCACCCGAACA+AGG | - | chr1.4:85486751-85486770 | MS.gene066075:CDS | 40.0% |
| !!! | TGTTTTTGGGTATCCAAGTG+AGG | + | chr1.4:85486896-85486915 | None:intergenic | 40.0% |
| AAATCTTCACCTTGTTCGGG+TGG | + | chr1.4:85486763-85486782 | None:intergenic | 45.0% | |
| GGTCAAATTGGAACCTCACT+TGG | - | chr1.4:85486880-85486899 | MS.gene066075:CDS | 45.0% | |
| TTTGCTGGTACATGTGGTGT+TGG | - | chr1.4:85486829-85486848 | MS.gene066075:CDS | 45.0% | |
| ! | GCTGGTACATGTGGTGTTGG+TGG | - | chr1.4:85486832-85486851 | MS.gene066075:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 85486739 | 85486966 | 85486739 | ID=MS.gene066075 |
| chr1.4 | mRNA | 85486739 | 85486966 | 85486739 | ID=MS.gene066075.t1;Parent=MS.gene066075 |
| chr1.4 | exon | 85486739 | 85486966 | 85486739 | ID=MS.gene066075.t1.exon1;Parent=MS.gene066075.t1 |
| chr1.4 | CDS | 85486739 | 85486966 | 85486739 | ID=cds.MS.gene066075.t1;Parent=MS.gene066075.t1 |
| Gene Sequence |
| Protein sequence |
