Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06615.t1 | AES72622.2 | 89.2 | 83 | 9 | 0 | 1 | 83 | 1 | 83 | 1.50E-33 | 151.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06615.t1 | G7J8Y9 | 89.2 | 83 | 9 | 0 | 1 | 83 | 1 | 83 | 1.1e-33 | 151.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057239 | MS.gene06615 | 0.923583 | 2.19E-89 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06615.t1 | MTR_3g092630 | 89.157 | 83 | 9 | 0 | 1 | 83 | 1 | 83 | 2.14e-51 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTTCGGTGGCTTCCTTAT+TGG | 0.147653 | 3.3:-77309965 | None:intergenic |
| CCAAGGAAAAGCTCCATTTC+TGG | 0.240987 | 3.3:-77309909 | None:intergenic |
| GCTTCGGTGGCTTCCTTATT+GGG | 0.250655 | 3.3:-77309964 | None:intergenic |
| TGGATCTTTCCCTGTGACTT+TGG | 0.318656 | 3.3:-77309986 | None:intergenic |
| TGTAAATTCAAAGCCCAATA+AGG | 0.347980 | 3.3:+77309951 | MS.gene06615:CDS |
| TTTCCCTGTGACTTTGGCTT+CGG | 0.384184 | 3.3:-77309980 | None:intergenic |
| CCCTGTGACTTTGGCTTCGG+TGG | 0.411077 | 3.3:-77309977 | None:intergenic |
| CAAGGAAAAGCTCCATTTCT+GGG | 0.412637 | 3.3:-77309908 | None:intergenic |
| AGATCTCACAGGCCCAGAAA+TGG | 0.436634 | 3.3:+77309896 | MS.gene06615:CDS |
| GCCACCGAAGCCAAAGTCAC+AGG | 0.448021 | 3.3:+77309976 | MS.gene06615:CDS |
| ACCTTTGTCTCGAAGGAGCC+GGG | 0.459192 | 3.3:-77310041 | None:intergenic |
| ACAAAGGTTTGCGCCGGAGT+TGG | 0.470643 | 3.3:+77310056 | MS.gene06615:CDS |
| CTTGGGTTTGAGCCCGGCGA+AGG | 0.495601 | 3.3:+77309927 | MS.gene06615:CDS |
| TTTGAATTTACATCCTTCGC+CGG | 0.498083 | 3.3:-77309940 | None:intergenic |
| AACCTTTGTCTCGAAGGAGC+CGG | 0.502714 | 3.3:-77310042 | None:intergenic |
| TTGAATTTACATCCTTCGCC+GGG | 0.526420 | 3.3:-77309939 | None:intergenic |
| GGCGCAAACCTTTGTCTCGA+AGG | 0.535403 | 3.3:-77310048 | None:intergenic |
| AGGTTTGCGCCGGAGTTGGA+TGG | 0.539550 | 3.3:+77310060 | MS.gene06615:CDS |
| TTCGAGACAAAGGTTTGCGC+CGG | 0.543692 | 3.3:+77310050 | MS.gene06615:CDS |
| TCCCGGCTCCTTCGAGACAA+AGG | 0.553672 | 3.3:+77310040 | MS.gene06615:CDS |
| TAGTGGAGACAAGCAAATCC+CGG | 0.571930 | 3.3:+77310023 | MS.gene06615:CDS |
| CTTCGCCGGGCTCAAACCCA+AGG | 0.573462 | 3.3:-77309926 | None:intergenic |
| GGGATTTGCTTGTCTCCACT+AGG | 0.582962 | 3.3:-77310021 | None:intergenic |
| CCACCGAAGCCAAAGTCACA+GGG | 0.585566 | 3.3:+77309977 | MS.gene06615:CDS |
| CAGTGTAGGCCATCCAACTC+CGG | 0.599129 | 3.3:-77310069 | None:intergenic |
| TCCATTACTCAGAAGTCAGA+AGG | 0.600136 | 3.3:-77310108 | None:intergenic |
| CAGCCACTTCGAGATCTCAC+AGG | 0.609143 | 3.3:+77309885 | MS.gene06615:CDS |
| CTATGAATTCAAAACAGTGT+AGG | 0.640211 | 3.3:-77310083 | None:intergenic |
| GGGCCTGTGAGATCTCGAAG+TGG | 0.708935 | 3.3:-77309888 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTATGAATTCAAAACAGTGT+AGG | - | chr3.3:77310086-77310105 | None:intergenic | 30.0% | |
| TGTAAATTCAAAGCCCAATA+AGG | + | chr3.3:77309951-77309970 | MS.gene06615:CDS | 30.0% | |
| TTTGAATTTACATCCTTCGC+CGG | - | chr3.3:77309943-77309962 | None:intergenic | 35.0% | |
| CAAGGAAAAGCTCCATTTCT+GGG | - | chr3.3:77309911-77309930 | None:intergenic | 40.0% | |
| CCAAAAACATCAACACCTAG+TGG | + | chr3.3:77310006-77310025 | MS.gene06615:CDS | 40.0% | |
| TTGAATTTACATCCTTCGCC+GGG | - | chr3.3:77309942-77309961 | None:intergenic | 40.0% | |
| ! | CAGAAATGGAGCTTTTCCTT+GGG | + | chr3.3:77309910-77309929 | MS.gene06615:CDS | 40.0% |
| !!! | CCACTAGGTGTTGATGTTTT+TGG | - | chr3.3:77310009-77310028 | None:intergenic | 40.0% |
| CCAAGGAAAAGCTCCATTTC+TGG | - | chr3.3:77309912-77309931 | None:intergenic | 45.0% | |
| TAGTGGAGACAAGCAAATCC+CGG | + | chr3.3:77310023-77310042 | MS.gene06615:CDS | 45.0% | |
| TGGATCTTTCCCTGTGACTT+TGG | - | chr3.3:77309989-77310008 | None:intergenic | 45.0% | |
| ! | CCAGAAATGGAGCTTTTCCT+TGG | + | chr3.3:77309909-77309928 | MS.gene06615:CDS | 45.0% |
| ! | TTTCCCTGTGACTTTGGCTT+CGG | - | chr3.3:77309983-77310002 | None:intergenic | 45.0% |
| AACCTTTGTCTCGAAGGAGC+CGG | - | chr3.3:77310045-77310064 | None:intergenic | 50.0% | |
| AGATCTCACAGGCCCAGAAA+TGG | + | chr3.3:77309896-77309915 | MS.gene06615:CDS | 50.0% | |
| GCTTCGGTGGCTTCCTTATT+GGG | - | chr3.3:77309967-77309986 | None:intergenic | 50.0% | |
| TTCGAGACAAAGGTTTGCGC+CGG | + | chr3.3:77310050-77310069 | MS.gene06615:CDS | 50.0% | |
| ! | CTTTTCCTTGGGTTTGAGCC+CGG | + | chr3.3:77309921-77309940 | MS.gene06615:CDS | 50.0% |
| ! | GGGATTTGCTTGTCTCCACT+AGG | - | chr3.3:77310024-77310043 | None:intergenic | 50.0% |
| ACAAAGGTTTGCGCCGGAGT+TGG | + | chr3.3:77310056-77310075 | MS.gene06615:CDS | 55.0% | |
| ACCTTTGTCTCGAAGGAGCC+GGG | - | chr3.3:77310044-77310063 | None:intergenic | 55.0% | |
| CAGCCACTTCGAGATCTCAC+AGG | + | chr3.3:77309885-77309904 | MS.gene06615:CDS | 55.0% | |
| CAGTGTAGGCCATCCAACTC+CGG | - | chr3.3:77310072-77310091 | None:intergenic | 55.0% | |
| CCACCGAAGCCAAAGTCACA+GGG | + | chr3.3:77309977-77309996 | MS.gene06615:CDS | 55.0% | |
| GGCGCAAACCTTTGTCTCGA+AGG | - | chr3.3:77310051-77310070 | None:intergenic | 55.0% | |
| GGCTTCGGTGGCTTCCTTAT+TGG | - | chr3.3:77309968-77309987 | None:intergenic | 55.0% | |
| AGGTTTGCGCCGGAGTTGGA+TGG | + | chr3.3:77310060-77310079 | MS.gene06615:CDS | 60.0% | |
| GCCACCGAAGCCAAAGTCAC+AGG | + | chr3.3:77309976-77309995 | MS.gene06615:CDS | 60.0% | |
| GGGCCTGTGAGATCTCGAAG+TGG | - | chr3.3:77309891-77309910 | None:intergenic | 60.0% | |
| TCCCGGCTCCTTCGAGACAA+AGG | + | chr3.3:77310040-77310059 | MS.gene06615:CDS | 60.0% | |
| ! | CCCTGTGACTTTGGCTTCGG+TGG | - | chr3.3:77309980-77309999 | None:intergenic | 60.0% |
| CTTCGCCGGGCTCAAACCCA+AGG | - | chr3.3:77309929-77309948 | None:intergenic | 65.0% | |
| CTTGGGTTTGAGCCCGGCGA+AGG | + | chr3.3:77309927-77309946 | MS.gene06615:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 77309868 | 77310122 | 77309868 | ID=MS.gene06615 |
| chr3.3 | mRNA | 77309868 | 77310122 | 77309868 | ID=MS.gene06615.t1;Parent=MS.gene06615 |
| chr3.3 | exon | 77309868 | 77310122 | 77309868 | ID=MS.gene06615.t1.exon1;Parent=MS.gene06615.t1 |
| chr3.3 | CDS | 77309868 | 77310122 | 77309868 | ID=cds.MS.gene06615.t1;Parent=MS.gene06615.t1 |
| Gene Sequence |
| Protein sequence |
