Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057239.t1 | AES72622.2 | 89.2 | 83 | 9 | 0 | 1 | 83 | 1 | 83 | 1.50E-33 | 151.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057239.t1 | G7J8Y9 | 89.2 | 83 | 9 | 0 | 1 | 83 | 1 | 83 | 1.1e-33 | 151.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057239 | MS.gene06615 | 0.923583 | 2.19E-89 | -1.69E-46 |
| MS.gene057239 | MS.gene38598 | 0.819145 | 1.35E-52 | -1.69E-46 |
| MS.gene057239 | MS.gene82041 | 0.847524 | 1.10E-59 | -1.69E-46 |
| MS.gene057239 | MS.gene82549 | 0.842592 | 2.35E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057239.t1 | MTR_3g092630 | 89.157 | 83 | 9 | 0 | 1 | 83 | 1 | 83 | 2.14e-51 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTTCGGTGGCTTCCTTAT+TGG | 0.147653 | 3.2:-77923966 | None:intergenic |
| CCAAGGAAAAGCTCCATTTC+TGG | 0.240987 | 3.2:-77923910 | None:intergenic |
| GCTTCGGTGGCTTCCTTATT+GGG | 0.250655 | 3.2:-77923965 | None:intergenic |
| TGGATCTTTCCCTGTGACTT+TGG | 0.318656 | 3.2:-77923987 | None:intergenic |
| TGTAAATTCAAAGCCCAATA+AGG | 0.347980 | 3.2:+77923952 | MS.gene057239:CDS |
| TTTCCCTGTGACTTTGGCTT+CGG | 0.384184 | 3.2:-77923981 | None:intergenic |
| CCCTGTGACTTTGGCTTCGG+TGG | 0.411077 | 3.2:-77923978 | None:intergenic |
| CAAGGAAAAGCTCCATTTCT+GGG | 0.412637 | 3.2:-77923909 | None:intergenic |
| AGATCTCACAGGCCCAGAAA+TGG | 0.436634 | 3.2:+77923897 | MS.gene057239:CDS |
| GCCACCGAAGCCAAAGTCAC+AGG | 0.448021 | 3.2:+77923977 | MS.gene057239:CDS |
| ACCTTTGTCTCGAAGGAGCC+GGG | 0.459192 | 3.2:-77924042 | None:intergenic |
| ACAAAGGTTTGCGCCGGAGT+TGG | 0.470643 | 3.2:+77924057 | MS.gene057239:CDS |
| CTTGGGTTTGAGCCCGGCGA+AGG | 0.495601 | 3.2:+77923928 | MS.gene057239:CDS |
| TTTGAATTTACATCCTTCGC+CGG | 0.498083 | 3.2:-77923941 | None:intergenic |
| AACCTTTGTCTCGAAGGAGC+CGG | 0.502714 | 3.2:-77924043 | None:intergenic |
| TTGAATTTACATCCTTCGCC+GGG | 0.526420 | 3.2:-77923940 | None:intergenic |
| GGCGCAAACCTTTGTCTCGA+AGG | 0.535403 | 3.2:-77924049 | None:intergenic |
| AGGTTTGCGCCGGAGTTGGA+TGG | 0.539550 | 3.2:+77924061 | MS.gene057239:CDS |
| TTCGAGACAAAGGTTTGCGC+CGG | 0.543692 | 3.2:+77924051 | MS.gene057239:CDS |
| TCCCGGCTCCTTCGAGACAA+AGG | 0.553672 | 3.2:+77924041 | MS.gene057239:CDS |
| TAGTGGAGACAAGCAAATCC+CGG | 0.571930 | 3.2:+77924024 | MS.gene057239:CDS |
| CTTCGCCGGGCTCAAACCCA+AGG | 0.573462 | 3.2:-77923927 | None:intergenic |
| GGGATTTGCTTGTCTCCACT+AGG | 0.582962 | 3.2:-77924022 | None:intergenic |
| CCACCGAAGCCAAAGTCACA+GGG | 0.585566 | 3.2:+77923978 | MS.gene057239:CDS |
| CAGTGTAGGCCATCCAACTC+CGG | 0.599129 | 3.2:-77924070 | None:intergenic |
| TCCATTACTCAGAAGTCAGA+AGG | 0.600136 | 3.2:-77924109 | None:intergenic |
| CAGCCACTTCGAGATCTCAC+AGG | 0.609143 | 3.2:+77923886 | MS.gene057239:CDS |
| CTATGAATTCAAAACAGTGT+AGG | 0.640211 | 3.2:-77924084 | None:intergenic |
| GGGCCTGTGAGATCTCGAAG+TGG | 0.708935 | 3.2:-77923889 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTATGAATTCAAAACAGTGT+AGG | - | chr3.2:77924087-77924106 | None:intergenic | 30.0% | |
| TGTAAATTCAAAGCCCAATA+AGG | + | chr3.2:77923952-77923971 | MS.gene057239:CDS | 30.0% | |
| TTTGAATTTACATCCTTCGC+CGG | - | chr3.2:77923944-77923963 | None:intergenic | 35.0% | |
| CAAGGAAAAGCTCCATTTCT+GGG | - | chr3.2:77923912-77923931 | None:intergenic | 40.0% | |
| CCAAAAACATCAACACCTAG+TGG | + | chr3.2:77924007-77924026 | MS.gene057239:CDS | 40.0% | |
| TTGAATTTACATCCTTCGCC+GGG | - | chr3.2:77923943-77923962 | None:intergenic | 40.0% | |
| ! | CAGAAATGGAGCTTTTCCTT+GGG | + | chr3.2:77923911-77923930 | MS.gene057239:CDS | 40.0% |
| !!! | CCACTAGGTGTTGATGTTTT+TGG | - | chr3.2:77924010-77924029 | None:intergenic | 40.0% |
| CCAAGGAAAAGCTCCATTTC+TGG | - | chr3.2:77923913-77923932 | None:intergenic | 45.0% | |
| TAGTGGAGACAAGCAAATCC+CGG | + | chr3.2:77924024-77924043 | MS.gene057239:CDS | 45.0% | |
| TGGATCTTTCCCTGTGACTT+TGG | - | chr3.2:77923990-77924009 | None:intergenic | 45.0% | |
| ! | CCAGAAATGGAGCTTTTCCT+TGG | + | chr3.2:77923910-77923929 | MS.gene057239:CDS | 45.0% |
| ! | TTTCCCTGTGACTTTGGCTT+CGG | - | chr3.2:77923984-77924003 | None:intergenic | 45.0% |
| AACCTTTGTCTCGAAGGAGC+CGG | - | chr3.2:77924046-77924065 | None:intergenic | 50.0% | |
| AGATCTCACAGGCCCAGAAA+TGG | + | chr3.2:77923897-77923916 | MS.gene057239:CDS | 50.0% | |
| GCTTCGGTGGCTTCCTTATT+GGG | - | chr3.2:77923968-77923987 | None:intergenic | 50.0% | |
| TTCGAGACAAAGGTTTGCGC+CGG | + | chr3.2:77924051-77924070 | MS.gene057239:CDS | 50.0% | |
| ! | CTTTTCCTTGGGTTTGAGCC+CGG | + | chr3.2:77923922-77923941 | MS.gene057239:CDS | 50.0% |
| ! | GGGATTTGCTTGTCTCCACT+AGG | - | chr3.2:77924025-77924044 | None:intergenic | 50.0% |
| ACAAAGGTTTGCGCCGGAGT+TGG | + | chr3.2:77924057-77924076 | MS.gene057239:CDS | 55.0% | |
| ACCTTTGTCTCGAAGGAGCC+GGG | - | chr3.2:77924045-77924064 | None:intergenic | 55.0% | |
| CAGCCACTTCGAGATCTCAC+AGG | + | chr3.2:77923886-77923905 | MS.gene057239:CDS | 55.0% | |
| CAGTGTAGGCCATCCAACTC+CGG | - | chr3.2:77924073-77924092 | None:intergenic | 55.0% | |
| CCACCGAAGCCAAAGTCACA+GGG | + | chr3.2:77923978-77923997 | MS.gene057239:CDS | 55.0% | |
| GGCGCAAACCTTTGTCTCGA+AGG | - | chr3.2:77924052-77924071 | None:intergenic | 55.0% | |
| GGCTTCGGTGGCTTCCTTAT+TGG | - | chr3.2:77923969-77923988 | None:intergenic | 55.0% | |
| AGGTTTGCGCCGGAGTTGGA+TGG | + | chr3.2:77924061-77924080 | MS.gene057239:CDS | 60.0% | |
| GCCACCGAAGCCAAAGTCAC+AGG | + | chr3.2:77923977-77923996 | MS.gene057239:CDS | 60.0% | |
| GGGCCTGTGAGATCTCGAAG+TGG | - | chr3.2:77923892-77923911 | None:intergenic | 60.0% | |
| TCCCGGCTCCTTCGAGACAA+AGG | + | chr3.2:77924041-77924060 | MS.gene057239:CDS | 60.0% | |
| ! | CCCTGTGACTTTGGCTTCGG+TGG | - | chr3.2:77923981-77924000 | None:intergenic | 60.0% |
| CTTCGCCGGGCTCAAACCCA+AGG | - | chr3.2:77923930-77923949 | None:intergenic | 65.0% | |
| CTTGGGTTTGAGCCCGGCGA+AGG | + | chr3.2:77923928-77923947 | MS.gene057239:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 77923869 | 77924123 | 77923869 | ID=MS.gene057239 |
| chr3.2 | mRNA | 77923869 | 77924123 | 77923869 | ID=MS.gene057239.t1;Parent=MS.gene057239 |
| chr3.2 | exon | 77923869 | 77924123 | 77923869 | ID=MS.gene057239.t1.exon1;Parent=MS.gene057239.t1 |
| chr3.2 | CDS | 77923869 | 77924123 | 77923869 | ID=cds.MS.gene057239.t1;Parent=MS.gene057239.t1 |
| Gene Sequence |
| Protein sequence |
