AlfalfaGEDB | MS.gene066165

M. sativa cultivar XinJiangDaYe / MS.gene066165

KEGG: -
GO: -
PFAM: PF12734.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066165.t1	PNX66177.1	88.5	78	9	0	1	78	1	78	3.20E-27	130.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066165.t1	A0A2K3KIR8	88.5	78	9	0	1	78	1	78	2.3e-27	130.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050363	MS.gene066165	0.821609	3.69E-53	-1.69E-46
MS.gene056408	MS.gene066165	0.816622	5.03E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066165.t1	MTR_2g069420	89.744	78	8	0	1	78	1	78	3.15e-39	124
MS.gene066165.t1	MTR_2g069430	91.026	78	5	1	1	78	1	76	1.67e-38	122
MS.gene066165.t1	MTR_1g040735	79.747	79	15	1	1	78	58	136	3.30e-36	119
MS.gene066165.t1	MTR_5g042360	78.481	79	16	1	1	78	1	79	4.45e-35	114
MS.gene066165.t1	MTR_1g040775	78.481	79	16	1	1	78	1	79	4.45e-35	114
MS.gene066165.t1	MTR_4g091090	83.750	80	11	1	1	78	1	80	7.60e-34	111
MS.gene066165.t1	MTR_2g069400	57.143	63	19	3	1	62	1	56	5.41e-14	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066165.t1	AT2G32190	50.000	78	32	4	1	78	1	71	1.34e-17	70.1
MS.gene066165.t1	AT2G32210	50.000	78	32	4	1	78	1	71	1.06e-16	67.8
MS.gene066165.t1	AT2G32200	43.210	81	36	5	1	78	1	74	2.22e-11	54.3
MS.gene066165.t1	AT2G32200	43.421	76	34	4	6	78	39	108	2.66e-11	55.1

Find 32 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTGGTGGTGGATATGATAC+TGG	0.277131	2.3:+26677305	None:intergenic
CATCACCCCTGCTAGTAGTT+TGG	0.398219	2.3:+26677183	None:intergenic
GGTCTGGAATCCTTTGTTGA+GGG	0.435636	2.3:+26677204	None:intergenic
CTGGTGGTGGATATGATACT+GGG	0.436288	2.3:+26677306	None:intergenic
GGTGGTGGGGCAGTTACATA+TGG	0.448724	2.3:+26677263	None:intergenic
GAAGGATAACCCATTGGTGG+TGG	0.456925	2.3:+26677248	None:intergenic
TGTAACTGCCCCACCACCAA+TGG	0.466214	2.3:-26677258	MS.gene066165:CDS
CCCCTGCTAGTAGTTTGGTC+TGG	0.475028	2.3:+26677188	None:intergenic
TAGCTTAAAAGCATATATCA+AGG	0.479451	2.3:+26677117	None:intergenic
TTCCAGACCAAACTACTAGC+AGG	0.528828	2.3:-26677190	MS.gene066165:CDS
GTAACTGCCCCACCACCAAT+GGG	0.530036	2.3:-26677257	MS.gene066165:CDS
TGGTCTGGAATCCTTTGTTG+AGG	0.537190	2.3:+26677203	None:intergenic
GAATAAGCCTCACCTGGTGG+TGG	0.537792	2.3:+26677293	None:intergenic
CCTTCCAAGGATGTCTCTGA+AGG	0.548959	2.3:-26677230	MS.gene066165:CDS
GTACCCTTCAGAGACATCCT+TGG	0.558950	2.3:+26677226	None:intergenic
AAGGATAACCCATTGGTGGT+GGG	0.561789	2.3:+26677249	None:intergenic
AGGATAACCCATTGGTGGTG+GGG	0.564664	2.3:+26677250	None:intergenic
TTGGAAGGATAACCCATTGG+TGG	0.578593	2.3:+26677245	None:intergenic
ACCAATGGGTTATCCTTCCA+AGG	0.585220	2.3:-26677243	MS.gene066165:CDS
CAAACTACTAGCAGGGGTGA+TGG	0.586318	2.3:-26677182	MS.gene066165:CDS
TCCAGACCAAACTACTAGCA+GGG	0.592593	2.3:-26677189	MS.gene066165:CDS
CCAGTATCATATCCACCACC+AGG	0.598096	2.3:-26677305	MS.gene066165:CDS
TCCTTGGAAGGATAACCCAT+TGG	0.604020	2.3:+26677242	None:intergenic
ATCATATCCACCACCAGGTG+AGG	0.605203	2.3:-26677300	MS.gene066165:CDS
GCTGAAGAATAAGCCTCACC+TGG	0.611288	2.3:+26677287	None:intergenic
CTTCCAAGGATGTCTCTGAA+GGG	0.620825	2.3:-26677229	MS.gene066165:CDS
AGCTTAAAAGCATATATCAA+GGG	0.622395	2.3:+26677118	None:intergenic
CCTTCAGAGACATCCTTGGA+AGG	0.637821	2.3:+26677230	None:intergenic
CCAGACCAAACTACTAGCAG+GGG	0.657589	2.3:-26677188	MS.gene066165:CDS
TGGTGGTGGATATGATACTG+GGG	0.671764	2.3:+26677307	None:intergenic
CTGAAGGGTACCCTCAACAA+AGG	0.674821	2.3:-26677214	MS.gene066165:CDS
GAAGAATAAGCCTCACCTGG+TGG	0.693478	2.3:+26677290	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTCCAAGGATGTCTCTGAA+GGG	-	chr2.3:26677227-26677246	MS.gene066165:CDS	45.0%
	TCCAGACCAAACTACTAGCA+GGG	-	chr2.3:26677267-26677286	MS.gene066165:CDS	45.0%
	TCCTTGGAAGGATAACCCAT+TGG	+	chr2.3:26677217-26677236	None:intergenic	45.0%
	TTCCAGACCAAACTACTAGC+AGG	-	chr2.3:26677266-26677285	MS.gene066165:CDS	45.0%
	TTGGAAGGATAACCCATTGG+TGG	+	chr2.3:26677214-26677233	None:intergenic	45.0%
!	AAGGATAACCCATTGGTGGT+GGG	+	chr2.3:26677210-26677229	None:intergenic	45.0%
!	GGTCTGGAATCCTTTGTTGA+GGG	+	chr2.3:26677255-26677274	None:intergenic	45.0%
!	TGGTCTGGAATCCTTTGTTG+AGG	+	chr2.3:26677256-26677275	None:intergenic	45.0%
!!	ACCAATGGGTTATCCTTCCA+AGG	-	chr2.3:26677213-26677232	MS.gene066165:CDS	45.0%
!!	AGGGGTGATGGTTTTTGGAA+GGG	-	chr2.3:26677286-26677305	MS.gene066165:CDS	45.0%
!!	CTGGTGGTGGATATGATACT+GGG	+	chr2.3:26677153-26677172	None:intergenic	45.0%
!!	TGGTGGTGGATATGATACTG+GGG	+	chr2.3:26677152-26677171	None:intergenic	45.0%
	ATCATATCCACCACCAGGTG+AGG	-	chr2.3:26677156-26677175	MS.gene066165:CDS	50.0%
	CAAACTACTAGCAGGGGTGA+TGG	-	chr2.3:26677274-26677293	MS.gene066165:CDS	50.0%
	CATCACCCCTGCTAGTAGTT+TGG	+	chr2.3:26677276-26677295	None:intergenic	50.0%
	CCAGACCAAACTACTAGCAG+GGG	-	chr2.3:26677268-26677287	MS.gene066165:CDS	50.0%
	CCAGTATCATATCCACCACC+AGG	-	chr2.3:26677151-26677170	MS.gene066165:CDS	50.0%
	CCTTCAGAGACATCCTTGGA+AGG	+	chr2.3:26677229-26677248	None:intergenic	50.0%
	CCTTCCAAGGATGTCTCTGA+AGG	-	chr2.3:26677226-26677245	MS.gene066165:CDS	50.0%
	GAAGAATAAGCCTCACCTGG+TGG	+	chr2.3:26677169-26677188	None:intergenic	50.0%
	GAAGGATAACCCATTGGTGG+TGG	+	chr2.3:26677211-26677230	None:intergenic	50.0%
	GCTGAAGAATAAGCCTCACC+TGG	+	chr2.3:26677172-26677191	None:intergenic	50.0%
	GTACCCTTCAGAGACATCCT+TGG	+	chr2.3:26677233-26677252	None:intergenic	50.0%
!!	AGGATAACCCATTGGTGGTG+GGG	+	chr2.3:26677209-26677228	None:intergenic	50.0%
!!	CAGGGGTGATGGTTTTTGGA+AGG	-	chr2.3:26677285-26677304	MS.gene066165:CDS	50.0%
!!	CCTGGTGGTGGATATGATAC+TGG	+	chr2.3:26677154-26677173	None:intergenic	50.0%
!!	CTAGCAGGGGTGATGGTTTT+TGG	-	chr2.3:26677281-26677300	MS.gene066165:CDS	50.0%
!!	CTGAAGGGTACCCTCAACAA+AGG	-	chr2.3:26677242-26677261	MS.gene066165:CDS	50.0%
	CCCCTGCTAGTAGTTTGGTC+TGG	+	chr2.3:26677271-26677290	None:intergenic	55.0%
	GAATAAGCCTCACCTGGTGG+TGG	+	chr2.3:26677166-26677185	None:intergenic	55.0%
	GTAACTGCCCCACCACCAAT+GGG	-	chr2.3:26677199-26677218	MS.gene066165:CDS	55.0%
	TGTAACTGCCCCACCACCAA+TGG	-	chr2.3:26677198-26677217	MS.gene066165:CDS	55.0%
!!	GGTGGTGGGGCAGTTACATA+TGG	+	chr2.3:26677196-26677215	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	26677121	26677357	26677121	ID=MS.gene066165
chr2.3	mRNA	26677121	26677357	26677121	ID=MS.gene066165.t1;Parent=MS.gene066165
chr2.3	exon	26677121	26677357	26677121	ID=MS.gene066165.t1.exon1;Parent=MS.gene066165.t1
chr2.3	CDS	26677121	26677357	26677121	ID=cds.MS.gene066165.t1;Parent=MS.gene066165.t1

Gene Sequence	>MS.gene066165 ATGAGTCACTTTAACAATCAGCAACAAACCCCAGTATCATATCCACCACCAGGTGAGGCTTATTCTTCAGCTCCATATGTAACTGCCCCACCACCAATGGGTTATCCTTCCAAGGATGTCTCTGAAGGGTACCCTCAACAAAGGATTCCAGACCAAACTACTAGCAGGGGTGATGGTTTTTGGAAGGGATGTTGTGCTGCTATATGTTGCTGTTGTGCCCTTGATATATGCTTTTAA
Protein sequence	>MS.gene066165.t1 MSHFNNQQQTPVSYPPPGEAYSSAPYVTAPPPMGYPSKDVSEGYPQQRIPDQTTSRGDGFWKGCCAAICCCCALDICF