Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066177.t1 | AFK36222.1 | 94.4 | 71 | 4 | 0 | 34 | 104 | 169 | 239 | 2.00E-25 | 125.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066177.t1 | Q8GX05 | 67.1 | 73 | 22 | 1 | 34 | 104 | 168 | 240 | 2.0e-18 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066177.t1 | G7L5U6 | 94.4 | 71 | 4 | 0 | 34 | 104 | 169 | 239 | 1.5e-25 | 125.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054236 | MS.gene066177 | 0.803703 | 3.07E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066177.t1 | MTR_7g089090 | 93.056 | 72 | 5 | 0 | 34 | 105 | 169 | 240 | 2.13e-37 | 133 |
| MS.gene066177.t1 | MTR_5g088860 | 58.333 | 72 | 30 | 0 | 34 | 105 | 168 | 239 | 1.85e-20 | 87.0 |
| MS.gene066177.t1 | MTR_5g088860 | 58.333 | 72 | 30 | 0 | 34 | 105 | 190 | 261 | 1.90e-20 | 87.0 |
| MS.gene066177.t1 | MTR_3g045180 | 55.556 | 72 | 32 | 0 | 34 | 105 | 164 | 235 | 1.46e-18 | 81.6 |
| MS.gene066177.t1 | MTR_6g079040 | 84.091 | 44 | 6 | 1 | 34 | 77 | 120 | 162 | 3.41e-15 | 69.3 |
| MS.gene066177.t1 | MTR_4g107580 | 84.375 | 32 | 5 | 0 | 5 | 36 | 568 | 599 | 2.78e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066177.t1 | AT3G56480 | 67.123 | 73 | 22 | 1 | 34 | 104 | 168 | 240 | 5.68e-25 | 99.8 |
| MS.gene066177.t1 | AT2G40820 | 69.444 | 72 | 20 | 1 | 34 | 103 | 61 | 132 | 6.35e-24 | 95.9 |
| MS.gene066177.t1 | AT2G40820 | 69.444 | 72 | 20 | 1 | 34 | 103 | 171 | 242 | 1.49e-23 | 95.5 |
| MS.gene066177.t1 | AT2G40820 | 69.444 | 72 | 20 | 1 | 34 | 103 | 171 | 242 | 1.49e-23 | 95.5 |
| MS.gene066177.t1 | AT2G40820 | 69.444 | 72 | 20 | 1 | 34 | 103 | 196 | 267 | 1.52e-23 | 95.9 |
| MS.gene066177.t1 | AT2G40820 | 69.444 | 72 | 20 | 1 | 34 | 103 | 183 | 254 | 1.59e-23 | 95.5 |
| MS.gene066177.t1 | AT2G26770 | 58.442 | 77 | 31 | 1 | 34 | 109 | 168 | 244 | 5.96e-21 | 88.2 |
| MS.gene066177.t1 | AT2G26770 | 58.442 | 77 | 31 | 1 | 34 | 109 | 168 | 244 | 5.96e-21 | 88.2 |
| MS.gene066177.t1 | AT2G26770 | 58.442 | 77 | 31 | 1 | 34 | 109 | 168 | 244 | 5.96e-21 | 88.2 |
Find 0 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCATTTATATATTCACAT+TGG | + | 15248:5971-5990 | MS.gene066177:intron | 15.0% |
| !! | ATATATGTTGAACTTTATGT+GGG | + | 15248:6735-6754 | MS.gene066177:intron | 20.0% |
| !! | GAACAAAAATATAGATCATA+TGG | - | 15248:6577-6596 | MS.gene066177:intergenic | 20.0% |
| !! | TGAATATATAAATGAAAGAG+AGG | - | 15248:5968-5987 | MS.gene066177:intergenic | 20.0% |
| ! | ATCATCATCAAAACTGTTAA+TGG | + | 15248:6236-6255 | MS.gene066177:intron | 25.0% |
| ! | ATGTAAATATTACTGTTTGC+AGG | + | 15248:6836-6855 | MS.gene066177:intron | 25.0% |
| ! | CATATATGTTGAACTTTATG+TGG | + | 15248:6734-6753 | MS.gene066177:intron | 25.0% |
| ! | CTAGCAGTGAAAAATAAATA+TGG | + | 15248:6145-6164 | MS.gene066177:intron | 25.0% |
| ! | TATGCAGAAAATTCATTTGA+AGG | - | 15248:6718-6737 | MS.gene066177:intergenic | 25.0% |
| ! | TTCTAAACCTGATTAAATGA+TGG | - | 15248:6799-6818 | MS.gene066177:intergenic | 25.0% |
| ! | TTGTAGATAACAGTAACAAA+TGG | - | 15248:5939-5958 | MS.gene066177:intergenic | 25.0% |
| ! | TTTATGCAGTTATCTATTCA+AGG | + | 15248:6880-6899 | MS.gene066177:CDS | 25.0% |
| !! | TCTTTTGTATCTTCATTACT+TGG | + | 15248:6074-6093 | MS.gene066177:intron | 25.0% |
| !!! | GATCTATATTTTTGTTCTTC+CGG | + | 15248:6580-6599 | MS.gene066177:intron | 25.0% |
| !!! | TGATTCCATTTTTGTTCTAT+AGG | + | 15248:5464-5483 | MS.gene066177:intron | 25.0% |
| AGAAGCCTATAGAACAAAAA+TGG | - | 15248:5472-5491 | MS.gene066177:intergenic | 30.0% | |
| AGTTAACGTAACATAATAGC+TGG | - | 15248:5786-5805 | MS.gene066177:intergenic | 30.0% | |
| ATTAAGCAATGTACCTTACT+TGG | - | 15248:5905-5924 | MS.gene066177:intergenic | 30.0% | |
| CATTACTTGGACTATGAATA+GGG | + | 15248:6087-6106 | MS.gene066177:intron | 30.0% | |
| CTCTATACCATCATTTAATC+AGG | + | 15248:6789-6808 | MS.gene066177:intron | 30.0% | |
| TAACAGCAAGAATTAGACAT+TGG | - | 15248:6469-6488 | MS.gene066177:intergenic | 30.0% | |
| TCATTACTTGGACTATGAAT+AGG | + | 15248:6086-6105 | MS.gene066177:intron | 30.0% | |
| TCTAGCATTATGCATTGTTT+GGG | + | 15248:6621-6640 | MS.gene066177:intron | 30.0% | |
| TTGATTATTGTGTCTTTGTC+TGG | + | 15248:6345-6364 | MS.gene066177:intron | 30.0% | |
| ! | ATTTTCTTGAAGCACAAAAC+TGG | - | 15248:6656-6675 | MS.gene066177:intergenic | 30.0% |
| ! | TATTGTTGTAGCACTTTTGA+TGG | + | 15248:5431-5450 | MS.gene066177:intron | 30.0% |
| ! | TTTTCTTGAAGCACAAAACT+GGG | - | 15248:6655-6674 | MS.gene066177:intergenic | 30.0% |
| !! | AAGTCTCTGAAATGTAAGAT+TGG | + | 15248:6398-6417 | MS.gene066177:CDS | 30.0% |
| !! | ATATTTTTGTTCTTCCGGAA+AGG | + | 15248:6585-6604 | MS.gene066177:intron | 30.0% |
| AATTGTGCAATCAAAGAGCA+TGG | - | 15248:5636-5655 | MS.gene066177:intergenic | 35.0% | |
| CTCTAGCATTATGCATTGTT+TGG | + | 15248:6620-6639 | MS.gene066177:intron | 35.0% | |
| GTCTAAACAATCTGAACCAA+AGG | + | 15248:5375-5394 | MS.gene066177:CDS | 35.0% | |
| TAAGGTGGTATAGGTACAAA+GGG | + | 15248:5305-5324 | MS.gene066177:CDS | 35.0% | |
| TTAAGGTGGTATAGGTACAA+AGG | + | 15248:5304-5323 | MS.gene066177:CDS | 35.0% | |
| TTAGTGGTGTTTAGTGGTTA+AGG | + | 15248:5398-5417 | MS.gene066177:intron | 35.0% | |
| ! | TTCTGAAGATGCTAAAAGGT+TGG | + | 15248:5488-5507 | MS.gene066177:CDS | 35.0% |
| ! | TTGTGTGTTTTCTTACTGTC+AGG | + | 15248:5820-5839 | MS.gene066177:intron | 35.0% |
| !! | TCTAATTCTTGCTGTTACTG+AGG | + | 15248:6472-6491 | MS.gene066177:intron | 35.0% |
| !! | TCTCTGAAATGTAAGATTGG+AGG | + | 15248:6401-6420 | MS.gene066177:CDS | 35.0% |
| AATGTAAGATTGGAGGTGAG+TGG | + | 15248:6408-6427 | MS.gene066177:CDS | 40.0% | |
| ACTGCATAAAAGCAGATAGC+AGG | - | 15248:6870-6889 | MS.gene066177:intergenic | 40.0% | |
| AGAGCAAGACAAAACCTTTC+CGG | - | 15248:6602-6621 | MS.gene066177:intergenic | 40.0% | |
| AGATACTATCCATCATCGCA+TGG | - | 15248:6539-6558 | MS.gene066177:intergenic | 40.0% | |
| AGGTACAAAGGGAATGATGA+TGG | + | 15248:5316-5335 | MS.gene066177:CDS | 40.0% | |
| ATGTAAGATTGGAGGTGAGT+GGG | + | 15248:6409-6428 | MS.gene066177:CDS | 40.0% | |
| CAAAGGTTAGTGGTGTTTAG+TGG | + | 15248:5392-5411 | MS.gene066177:intron | 40.0% | |
| CAATCTGAACCAAAGGTTAG+TGG | + | 15248:5382-5401 | MS.gene066177:intron | 40.0% | |
| CACTAAACACCACTAACCTT+TGG | - | 15248:5394-5413 | MS.gene066177:intergenic | 40.0% | |
| GTGATCTTAGTTAGCTGATG+AGG | - | 15248:6320-6339 | MS.gene066177:intergenic | 40.0% | |
| TACACAAGCTTCTAAACCAC+AGG | + | 15248:5605-5624 | MS.gene066177:CDS | 40.0% | |
| ! | AAAGAGCATGGTAAAACCTG+TGG | - | 15248:5624-5643 | MS.gene066177:intergenic | 40.0% |
| ! | AGGCTTCTGAAGATGCTAAA+AGG | + | 15248:5484-5503 | MS.gene066177:intron | 40.0% |
| ! | GTTTTCTTACTGTCAGGATG+TGG | + | 15248:5826-5845 | MS.gene066177:intron | 40.0% |
| ! | TTTTAACAGAAGCCTGACCA+TGG | - | 15248:6290-6309 | MS.gene066177:intergenic | 40.0% |
| !! | GATGGTACTGATCAAGTTAG+TGG | + | 15248:5334-5353 | MS.gene066177:CDS | 40.0% |
| !! | GCAGTTGCAGTTTGATTTGA+AGG | + | 15248:6676-6695 | MS.gene066177:intron | 40.0% |
| ATTGCTGCATCAGCCAAGTA+AGG | + | 15248:5889-5908 | MS.gene066177:CDS | 45.0% | |
| TCTTACTGTCAGGATGTGGA+TGG | + | 15248:5830-5849 | MS.gene066177:intron | 45.0% | |
| TGCTCTAGCACTCTCGATTT+CGG | - | 15248:5541-5560 | MS.gene066177:intergenic | 45.0% | |
| TGGCAAGCTTAAGGTGGTAT+AGG | + | 15248:5296-5315 | MS.gene066177:CDS | 45.0% | |
| TGTAAGATTGGAGGTGAGTG+GGG | + | 15248:6410-6429 | MS.gene066177:CDS | 45.0% | |
| !! | TCTTGCTGTTACTGAGGAGA+GGG | + | 15248:6478-6497 | MS.gene066177:intron | 45.0% |
| !! | TTCTTGCTGTTACTGAGGAG+AGG | + | 15248:6477-6496 | MS.gene066177:intron | 45.0% |
| ACTGGTGGAAGAGGTTCAAG+AGG | + | 15248:5853-5872 | MS.gene066177:CDS | 50.0% | |
| GAATGCTTTGCAAAGCACGC+AGG | + | 15248:6432-6451 | MS.gene066177:CDS | 50.0% | |
| GTTCGTTGACCATGCGATGA+TGG | + | 15248:6527-6546 | MS.gene066177:intron | 50.0% | |
| ! | TTTTCAACGCAGCTCTGCCA+TGG | + | 15248:6270-6289 | MS.gene066177:intron | 50.0% |
| !! | AATCGAGAGTGCTAGAGCAG+TGG | + | 15248:5542-5561 | MS.gene066177:CDS | 50.0% |
| AGTGCTAGAGCAGTGGTGCT+GGG | + | 15248:5549-5568 | MS.gene066177:CDS | 55.0% | |
| CTGTCAGGATGTGGATGGAC+TGG | + | 15248:5835-5854 | MS.gene066177:intron | 55.0% | |
| ! | AGAGCAGTGGTGCTGGGAAT+TGG | + | 15248:5555-5574 | MS.gene066177:CDS | 55.0% |
| ! | AGCAGTGGTGCTGGGAATTG+GGG | + | 15248:5557-5576 | MS.gene066177:CDS | 55.0% |
| ! | GAGCAGTGGTGCTGGGAATT+GGG | + | 15248:5556-5575 | MS.gene066177:CDS | 55.0% |
| !! | TCAGGATGTGGATGGACTGG+TGG | + | 15248:5838-5857 | MS.gene066177:intron | 55.0% |
| !! | TGTGGATGGACTGGTGGAAG+AGG | + | 15248:5844-5863 | MS.gene066177:CDS | 55.0% |
| AACGCAGCTCTGCCATGGTC+AGG | + | 15248:6275-6294 | MS.gene066177:intron | 60.0% | |
| GAGTGCTAGAGCAGTGGTGC+TGG | + | 15248:5548-5567 | MS.gene066177:CDS | 60.0% | |
| ! | AGTGGTGCTGGGAATTGGGG+AGG | + | 15248:5560-5579 | MS.gene066177:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15248 | gene | 5295 | 6918 | 5295 | ID=MS.gene066177 |
| 15248 | mRNA | 5295 | 6918 | 5295 | ID=MS.gene066177.t1;Parent=MS.gene066177 |
| 15248 | exon | 5295 | 5396 | 5295 | ID=MS.gene066177.t1.exon1;Parent=MS.gene066177.t1 |
| 15248 | CDS | 5295 | 5396 | 5295 | ID=cds.MS.gene066177.t1;Parent=MS.gene066177.t1 |
| 15248 | exon | 5486 | 5626 | 5486 | ID=MS.gene066177.t1.exon2;Parent=MS.gene066177.t1 |
| 15248 | CDS | 5486 | 5626 | 5486 | ID=cds.MS.gene066177.t1;Parent=MS.gene066177.t1 |
| 15248 | exon | 5842 | 5910 | 5842 | ID=MS.gene066177.t1.exon3;Parent=MS.gene066177.t1 |
| 15248 | CDS | 5842 | 5910 | 5842 | ID=cds.MS.gene066177.t1;Parent=MS.gene066177.t1 |
| 15248 | exon | 6371 | 6453 | 6371 | ID=MS.gene066177.t1.exon4;Parent=MS.gene066177.t1 |
| 15248 | CDS | 6371 | 6453 | 6371 | ID=cds.MS.gene066177.t1;Parent=MS.gene066177.t1 |
| 15248 | exon | 6858 | 6918 | 6858 | ID=MS.gene066177.t1.exon5;Parent=MS.gene066177.t1 |
| 15248 | CDS | 6858 | 6918 | 6858 | ID=cds.MS.gene066177.t1;Parent=MS.gene066177.t1 |
| Gene Sequence |
| Protein sequence |
