AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066204

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066204.t1 MTR_2g067640 96.858 541 17 0 1 541 1 541 0.0 1070
MS.gene066204.t1 MTR_2g062430 61.302 553 189 8 3 541 12 553 0.0 672
MS.gene066204.t1 MTR_2g062410 58.545 550 200 8 10 541 47 586 0.0 645
MS.gene066204.t1 MTR_2g062370 55.738 549 211 8 15 541 52 590 0.0 615
MS.gene066204.t1 MTR_4g131710 56.600 553 202 10 15 541 46 586 0.0 614
MS.gene066204.t1 MTR_2g062340 55.898 551 196 9 1 540 2 516 0.0 599
MS.gene066204.t1 MTR_2g062380 53.618 539 219 8 24 541 32 560 0.0 573
MS.gene066204.t1 MTR_4g077620 47.509 562 266 9 3 540 11 567 5.62e-179 516
MS.gene066204.t1 MTR_5g007860 47.913 551 255 8 19 540 58 605 3.24e-173 503
MS.gene066204.t1 MTR_1g080370 45.652 552 263 10 13 540 20 558 7.95e-169 490
MS.gene066204.t1 MTR_1g080410 45.652 552 263 10 13 540 20 558 7.95e-169 490
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066204.t1 AT2G44230 56.115 556 215 9 1 541 1 542 0.0 628
MS.gene066204.t1 AT2G44260 55.160 562 220 8 1 540 1 552 0.0 625
MS.gene066204.t1 AT2G44260 52.365 592 220 9 1 540 1 582 0.0 610
MS.gene066204.t1 AT3G01880 50.275 545 242 9 14 540 56 589 0.0 543
MS.gene066204.t1 AT3G01870 50.184 544 242 8 14 540 52 583 0.0 537
MS.gene066204.t1 AT5G43950 47.818 550 263 10 13 540 17 564 8.27e-174 503
MS.gene066204.t1 AT5G18490 45.956 544 256 11 20 541 25 552 2.24e-165 481
MS.gene066204.t1 AT1G04090 45.893 560 265 14 13 540 17 570 5.84e-162 473
MS.gene066204.t1 AT3G04350 44.029 561 277 12 6 541 18 566 5.90e-161 470
MS.gene066204.t1 AT3G04350 44.052 538 270 11 29 541 13 544 2.51e-155 455

Find 127 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAAATTACTTATAATTT+AGG 0.099121 2.2:+25498893 MS.gene066204:CDS
AATTGTTTCTGGGGAGTATT+TGG 0.142107 2.2:+25498820 MS.gene066204:CDS
AGTGATTTGGGTATTGATTT+TGG 0.158866 2.2:+25498785 MS.gene066204:CDS
CCTAATGGTTATAGCCCTTT+AGG 0.202069 2.2:+25497831 MS.gene066204:CDS
TGAGATTAATTCATGGATTT+GGG 0.203465 2.2:+25497932 MS.gene066204:CDS
GTGAGATTAATTCATGGATT+TGG 0.207795 2.2:+25497931 MS.gene066204:CDS
GCCTAATTTACCCCAAATTA+AGG 0.223955 2.2:+25498136 MS.gene066204:CDS
CTTGTGCTAGTGCCTTAATT+TGG 0.229784 2.2:-25498148 None:intergenic
GCCTTAATTTGGGGTAAATT+AGG 0.239469 2.2:-25498137 None:intergenic
TCTCTATTAGCTTTATCTTT+TGG 0.243499 2.2:-25498356 None:intergenic
GTGGCTTATTCTTCATTAAA+TGG 0.252619 2.2:+25498686 MS.gene066204:CDS
TTGTGCTAGTGCCTTAATTT+GGG 0.273123 2.2:-25498147 None:intergenic
TGAAGAACCAATCCAGCTTT+TGG 0.276809 2.2:-25498722 None:intergenic
TGCTCCATTTGACTCATCTT+TGG 0.277282 2.2:-25497715 None:intergenic
AATGTGCCAGGCCCATTAAA+AGG 0.295091 2.2:-25498479 None:intergenic
GTCATCTTGTCCACCTTCTA+AGG 0.303756 2.2:-25497586 None:intergenic
AGGTGATGAGTTGGAGAAGT+TGG 0.308524 2.2:+25498913 MS.gene066204:CDS
GACCTAAGCAGAAGAGAAAT+TGG 0.314600 2.2:+25498996 MS.gene066204:CDS
TGAAACTAAGAAGAGTGATT+TGG 0.317355 2.2:+25498772 MS.gene066204:CDS
ATCAAATGGTATTTCACAAA+TGG 0.321572 2.2:+25498224 MS.gene066204:CDS
CTAATGGTTATAGCCCTTTA+GGG 0.328617 2.2:+25497832 MS.gene066204:CDS
CCAAACAACAAACCTCTCTT+TGG 0.335542 2.2:+25497678 MS.gene066204:CDS
CCAAAGAGAGGTTTGTTGTT+TGG 0.338726 2.2:-25497678 None:intergenic
GAAGCAATGATGGTGCTTAT+TGG 0.344274 2.2:+25498324 MS.gene066204:CDS
CAATTGATTACACACTTGTT+TGG 0.344510 2.2:+25497748 MS.gene066204:CDS
CAAGCTCCTAGTGTTCTTGT+TGG 0.352506 2.2:+25498023 MS.gene066204:CDS
CCTCAAGGTTTCTTCACATT+AGG 0.352539 2.2:+25497642 MS.gene066204:CDS
TATCAACATTCAACGAAATC+TGG 0.353989 2.2:+25497559 MS.gene066204:CDS
GAAACTAAGAAGAGTGATTT+GGG 0.354458 2.2:+25498773 MS.gene066204:CDS
TGAGGAAGATTTGAGCCTAA+TGG 0.365782 2.2:-25498296 None:intergenic
ATTGTTTCTGGGGAGTATTT+GGG 0.370952 2.2:+25498821 MS.gene066204:CDS
TGGTGGAACATTTACTGATT+TGG 0.371624 2.2:+25498439 MS.gene066204:CDS
TTCAACCATGTTCAATCAAA+TGG 0.371740 2.2:+25498210 MS.gene066204:CDS
GGTAATGGAATCATTGATCT+AGG 0.375117 2.2:+25497516 MS.gene066204:CDS
GTTGGAGAAGTTGGAGAAGT+TGG 0.375686 2.2:+25498922 MS.gene066204:CDS
AACATTTCACTTGGTAAGAT+AGG 0.380922 2.2:+25498521 MS.gene066204:CDS
CAAACAAGTGTGTAATCAAT+TGG 0.381962 2.2:-25497747 None:intergenic
TCAAATGGTATTTCACAAAT+GGG 0.383417 2.2:+25498225 MS.gene066204:CDS
TTCTTCACCTATCAACTCAT+TGG 0.384297 2.2:-25498963 None:intergenic
AAGATAAAGATGGTTACATT+TGG 0.391775 2.2:+25497796 MS.gene066204:CDS
AAGTTGGAGAAGTTGGTTCC+TGG 0.393530 2.2:+25498929 MS.gene066204:CDS
TTTCATGTAAAACCAATGTT+TGG 0.395827 2.2:+25498419 MS.gene066204:CDS
CAACAAACCTCTCTTTGGTT+GGG 0.398679 2.2:+25497683 MS.gene066204:CDS
CTTCAAGGTACTAGTGAGAT+TGG 0.399867 2.2:+25498740 MS.gene066204:CDS
TTCAAACAAGAAATAGGAAG+AGG 0.408402 2.2:-25498083 None:intergenic
CTCTTCTGCTTAGGTCCAGT+GGG 0.408957 2.2:-25498989 None:intergenic
GAAATCTGGACAACCTTAGA+AGG 0.413008 2.2:+25497573 MS.gene066204:CDS
ACCAATGTGAGATTAATTCA+TGG 0.416401 2.2:+25497925 MS.gene066204:CDS
ACAACAAACCTCTCTTTGGT+TGG 0.417138 2.2:+25497682 MS.gene066204:CDS
CACAACATAGTAATGGTCAA+TGG 0.421062 2.2:+25498621 MS.gene066204:CDS
TGGTTACCAATAGCACCTAA+TGG 0.422006 2.2:+25497816 MS.gene066204:CDS
CTATAACCATTAGGTGCTAT+TGG 0.424957 2.2:-25497822 None:intergenic
GTTTGTATAGGTGGTGGATT+TGG 0.429168 2.2:+25497495 MS.gene066204:intron
GCTTGAATACCTCTATTGAT+TGG 0.429359 2.2:-25498005 None:intergenic
GCAACTTTCTTTGAACCAAC+AGG 0.429396 2.2:+25497615 MS.gene066204:CDS
TAGGTGAACATATTGGTGAT+TGG 0.434346 2.2:+25498540 MS.gene066204:CDS
AGGTGAACATATTGGTGATT+GGG 0.441048 2.2:+25498541 MS.gene066204:CDS
CCTAAAGGGCTATAACCATT+AGG 0.444178 2.2:-25497831 None:intergenic
CCTAATGGATCAATTTCCAT+TGG 0.445375 2.2:-25498281 None:intergenic
TGTTGTTTGGTTGACTATAG+TGG 0.446756 2.2:-25497665 None:intergenic
TATTTATCCAAAAGCTGGAT+TGG 0.447098 2.2:+25498715 MS.gene066204:CDS
CATGTAAAACCAATGTTTGG+TGG 0.453406 2.2:+25498422 MS.gene066204:CDS
ATCAAATTGTTGAGCCAGGT+TGG 0.455958 2.2:+25498846 MS.gene066204:CDS
CATGCTATTTATCCAAAAGC+TGG 0.456591 2.2:+25498710 MS.gene066204:CDS
AAAGCTGGATTGGTTCTTCA+AGG 0.458586 2.2:+25498725 MS.gene066204:CDS
AACCAATTTCTCTTCTGCTT+AGG 0.465571 2.2:-25498998 None:intergenic
GGGGCATTGCTTTATAAGAA+AGG 0.466177 2.2:+25498245 MS.gene066204:CDS
TTAAGAGTAAGTAACTTCAA+TGG 0.466888 2.2:+25498575 MS.gene066204:CDS
TTATAATTTAGGTGATGAGT+TGG 0.471376 2.2:+25498904 MS.gene066204:CDS
TCAAATTGTTGAGCCAGGTT+GGG 0.471665 2.2:+25498847 MS.gene066204:CDS
GGGCCTGGCACATTAAAAGT+TGG 0.476627 2.2:+25498488 MS.gene066204:CDS
CCTCAAGGTGGAAGCAATGA+TGG 0.478383 2.2:+25498314 MS.gene066204:CDS
ACAACATAGTAATGGTCAAT+GGG 0.479231 2.2:+25498622 MS.gene066204:CDS
TGAGTACCTTGTGGCAAAGT+TGG 0.484565 2.2:-25497301 None:intergenic
CCAATGGAAATTGATCCATT+AGG 0.485356 2.2:+25498281 MS.gene066204:CDS
GGAAATCAAATTGTTGAGCC+AGG 0.489502 2.2:+25498842 MS.gene066204:CDS
TCTTCACCTATCAACTCATT+GGG 0.491681 2.2:-25498962 None:intergenic
GTTGTTACAATATGCCCTAA+AGG 0.493241 2.2:-25497846 None:intergenic
GGCCTGGCACATTAAAAGTT+GGG 0.493640 2.2:+25498489 MS.gene066204:CDS
GGTAAGATAGGTGAACATAT+TGG 0.495274 2.2:+25498533 MS.gene066204:CDS
CTCATAGATAACATTTCACT+TGG 0.495794 2.2:+25498512 MS.gene066204:CDS
AAGCTTAAATGTGGTGTCAA+TGG 0.498242 2.2:-25497266 None:intergenic
GGCAAAGTTGGTATGTTAGC+TGG 0.501820 2.2:-25497289 None:intergenic
TCTCTTCTGCTTAGGTCCAG+TGG 0.506056 2.2:-25498990 None:intergenic
ATGTGCCAGGCCCATTAAAA+GGG 0.511536 2.2:-25498478 None:intergenic
AAGCAGAAGAGAAATTGGTT+TGG 0.512029 2.2:+25499001 MS.gene066204:CDS
TTGTTACAATATGCCCTAAA+GGG 0.516168 2.2:-25497845 None:intergenic
ATAGGTGGTGGATTTGGTAA+TGG 0.516311 2.2:+25497501 MS.gene066204:intron
TTAGGATGCAAGTACATGAA+TGG 0.525866 2.2:-25498179 None:intergenic
AGCTTAAATGTGGTGTCAAT+GGG 0.528640 2.2:-25497265 None:intergenic
TCATTGGGCAATTTCAAGCC+AGG 0.529228 2.2:-25498947 None:intergenic
GTTGCCAAAGATGAGTCAAA+TGG 0.531167 2.2:+25497711 MS.gene066204:CDS
TTTAATGAAGAATAAGCCAC+TGG 0.534311 2.2:-25498683 None:intergenic
GAAGGTGGACAAGATGACTT+AGG 0.543468 2.2:+25497591 MS.gene066204:CDS
AATGAGTTGATAGGTGAAGA+AGG 0.545267 2.2:+25498965 MS.gene066204:CDS
AAACCTTGAGGTATTCCTGT+TGG 0.546596 2.2:-25497630 None:intergenic
ATAGGTGAAGAAGGGCCCAC+TGG 0.547362 2.2:+25498974 MS.gene066204:CDS
AAAATCAAACAAGATAAAGA+TGG 0.551254 2.2:+25497786 MS.gene066204:CDS
AAATTGCCCAATGAGTTGAT+AGG 0.552488 2.2:+25498956 MS.gene066204:CDS
CAATGATGGTGCTTATTGGT+TGG 0.558172 2.2:+25498328 MS.gene066204:CDS
TCAGAAGTTGAATTTCAAAG+TGG 0.558316 2.2:+25498653 MS.gene066204:CDS
GTAAATGTTCCACCAAACAT+TGG 0.562694 2.2:-25498431 None:intergenic
TCAAACAAGAAATAGGAAGA+GGG 0.564288 2.2:-25498082 None:intergenic
AGGAGAAGAATCAAATCCAA+TGG 0.569336 2.2:+25498265 MS.gene066204:CDS
CATTTACTGATTTGGCCATG+TGG 0.588074 2.2:+25498447 MS.gene066204:CDS
TATGTTAGACCAATCAATAG+AGG 0.593203 2.2:+25497996 MS.gene066204:CDS
GAACCAACAGGAATACCTCA+AGG 0.594921 2.2:+25497627 MS.gene066204:CDS
AATGTGGTGTCAATGGGAAG+AGG 0.596119 2.2:-25497259 None:intergenic
AAAGTTCCAACAAGAACACT+AGG 0.596544 2.2:-25498029 None:intergenic
TGTGCTAGTGCCTTAATTTG+GGG 0.596705 2.2:-25498146 None:intergenic
CCATCATTGCTTCCACCTTG+AGG 0.597806 2.2:-25498314 None:intergenic
ATGAGTTGATAGGTGAAGAA+GGG 0.608495 2.2:+25498966 MS.gene066204:CDS
CATTGGATTCTATCAAGTGA+AGG 0.610351 2.2:-25497882 None:intergenic
TTAGGCTCAAATCTTCCTCA+AGG 0.614815 2.2:+25498299 MS.gene066204:CDS
GAGATTAATTCATGGATTTG+GGG 0.616547 2.2:+25497933 MS.gene066204:CDS
GGCTCAAATCTTCCTCAAGG+TGG 0.618174 2.2:+25498302 MS.gene066204:CDS
AAATACCATTTGATTGAACA+TGG 0.621650 2.2:-25498215 None:intergenic
ATCTGGACAACCTTAGAAGG+TGG 0.623634 2.2:+25497576 MS.gene066204:CDS
TCCATGAATTAATCTCACAT+TGG 0.628335 2.2:-25497926 None:intergenic
CAAATGGTATTTCACAAATG+GGG 0.632002 2.2:+25498226 MS.gene066204:CDS
AACATACCAACTTTGCCACA+AGG 0.638221 2.2:+25497295 MS.gene066204:CDS
TGAGATCTGATCTCACACAT+TGG 0.647996 2.2:-25497899 None:intergenic
ACTAGAACCCAACCAAAGAG+AGG 0.659753 2.2:-25497690 None:intergenic
TCAAAGTGGAAATAAACCAG+TGG 0.680780 2.2:+25498667 MS.gene066204:CDS
AATGGAATCATTGATCTAGG+TGG 0.694511 2.2:+25497519 MS.gene066204:CDS
CCTAATGTGAAGAAACCTTG+AGG 0.698728 2.2:-25497642 None:intergenic
GTTAGCTGGAAGCTTAAATG+TGG 0.712615 2.2:-25497275 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCTAAAATTACTTATAATTT+AGG + chr2.2:25498893-25498912 MS.gene066204:CDS 15.0%
!! GATAAAAAAATTGATGAAAA+AGG + chr2.2:25497960-25497979 MS.gene066204:CDS 15.0%
!!! CCTAAATTATAAGTAATTTT+AGG - chr2.2:25498896-25498915 None:intergenic 15.0%
!!! TTAATTTTGAAATTGTTTCT+GGG + chr2.2:25498810-25498829 MS.gene066204:CDS 15.0%
!! AAAATCAAACAAGATAAAGA+TGG + chr2.2:25497786-25497805 MS.gene066204:CDS 20.0%
!!! AGATTTTTCAAACAAGAAAT+AGG - chr2.2:25498092-25498111 None:intergenic 20.0%
!!! GTTAATTTTGAAATTGTTTC+TGG + chr2.2:25498809-25498828 MS.gene066204:CDS 20.0%
!!! TAATTTTGAAATTGTTTCTG+GGG + chr2.2:25498811-25498830 MS.gene066204:CDS 20.0%
!!! TATTTTTCACAACATAGTAA+TGG + chr2.2:25498614-25498633 MS.gene066204:CDS 20.0%
! AAATACCATTTGATTGAACA+TGG - chr2.2:25498218-25498237 None:intergenic 25.0%
! AAGATAAAGATGGTTACATT+TGG + chr2.2:25497796-25497815 MS.gene066204:CDS 25.0%
! AATAGAGAAAGAGTCAAAAA+AGG + chr2.2:25498371-25498390 MS.gene066204:CDS 25.0%
! ATCAAATGGTATTTCACAAA+TGG + chr2.2:25498224-25498243 MS.gene066204:CDS 25.0%
! TCAAATGGTATTTCACAAAT+GGG + chr2.2:25498225-25498244 MS.gene066204:CDS 25.0%
! TGAGATTAATTCATGGATTT+GGG + chr2.2:25497932-25497951 MS.gene066204:CDS 25.0%
! TTAAGAGTAAGTAACTTCAA+TGG + chr2.2:25498575-25498594 MS.gene066204:CDS 25.0%
! TTATAATTTAGGTGATGAGT+TGG + chr2.2:25498904-25498923 MS.gene066204:CDS 25.0%
! TTTCATGTAAAACCAATGTT+TGG + chr2.2:25498419-25498438 MS.gene066204:CDS 25.0%
!! TTGATATTTGTGACACTTTT+AGG - chr2.2:25497545-25497564 None:intergenic 25.0%
!!! GGATTTTTTACCCTTTTAAT+GGG + chr2.2:25498468-25498487 MS.gene066204:CDS 25.0%
!!! GGTGAATTTTTTTAGACAAT+GGG + chr2.2:25498868-25498887 MS.gene066204:CDS 25.0%
!!! GTGAATTTTTTTAGACAATG+GGG + chr2.2:25498869-25498888 MS.gene066204:CDS 25.0%
!!! TCTCTATTAGCTTTATCTTT+TGG - chr2.2:25498359-25498378 None:intergenic 25.0%
!!! TGGATTTTTTACCCTTTTAA+TGG + chr2.2:25498467-25498486 MS.gene066204:CDS 25.0%
AAAAGGGTAAAAAATCCACA+TGG - chr2.2:25498465-25498484 None:intergenic 30.0%
AACATTTCACTTGGTAAGAT+AGG + chr2.2:25498521-25498540 MS.gene066204:CDS 30.0%
AACTTTGCATGTGTTTGTAT+AGG + chr2.2:25497483-25497502 MS.gene066204:intron 30.0%
ACAACATAGTAATGGTCAAT+GGG + chr2.2:25498622-25498641 MS.gene066204:CDS 30.0%
ACCAATGTGAGATTAATTCA+TGG + chr2.2:25497925-25497944 MS.gene066204:CDS 30.0%
CAAACAAGTGTGTAATCAAT+TGG - chr2.2:25497750-25497769 None:intergenic 30.0%
CAAATGGTATTTCACAAATG+GGG + chr2.2:25498226-25498245 MS.gene066204:CDS 30.0%
CAATTGATTACACACTTGTT+TGG + chr2.2:25497748-25497767 MS.gene066204:CDS 30.0%
CTCATAGATAACATTTCACT+TGG + chr2.2:25498512-25498531 MS.gene066204:CDS 30.0%
GAAACTAAGAAGAGTGATTT+GGG + chr2.2:25498773-25498792 MS.gene066204:CDS 30.0%
GAGATTAATTCATGGATTTG+GGG + chr2.2:25497933-25497952 MS.gene066204:CDS 30.0%
GATAATTTCTTGTCGGAATT+TGG - chr2.2:25497359-25497378 None:intergenic 30.0%
GGTGATTTCAAAAATTGTCA+AGG + chr2.2:25498392-25498411 MS.gene066204:CDS 30.0%
GTGAGATTAATTCATGGATT+TGG + chr2.2:25497931-25497950 MS.gene066204:CDS 30.0%
GTGGCTTATTCTTCATTAAA+TGG + chr2.2:25498686-25498705 MS.gene066204:CDS 30.0%
TATCAACATTCAACGAAATC+TGG + chr2.2:25497559-25497578 MS.gene066204:CDS 30.0%
TATGTTAGACCAATCAATAG+AGG + chr2.2:25497996-25498015 MS.gene066204:CDS 30.0%
TATTTATCCAAAAGCTGGAT+TGG + chr2.2:25498715-25498734 MS.gene066204:CDS 30.0%
TCAAACAAGAAATAGGAAGA+GGG - chr2.2:25498085-25498104 None:intergenic 30.0%
TCAGAAGTTGAATTTCAAAG+TGG + chr2.2:25498653-25498672 MS.gene066204:CDS 30.0%
TCCATGAATTAATCTCACAT+TGG - chr2.2:25497929-25497948 None:intergenic 30.0%
TGAAACTAAGAAGAGTGATT+TGG + chr2.2:25498772-25498791 MS.gene066204:CDS 30.0%
TGGTGAAAATACTATCAAGA+AGG - chr2.2:25497339-25497358 None:intergenic 30.0%
TTCAAACAAGAAATAGGAAG+AGG - chr2.2:25498086-25498105 None:intergenic 30.0%
TTCAACCATGTTCAATCAAA+TGG + chr2.2:25498210-25498229 MS.gene066204:CDS 30.0%
TTGTTACAATATGCCCTAAA+GGG - chr2.2:25497848-25497867 None:intergenic 30.0%
TTTAATGAAGAATAAGCCAC+TGG - chr2.2:25498686-25498705 None:intergenic 30.0%
! CATGGTTGAAACTTTTCATT+AGG - chr2.2:25498200-25498219 None:intergenic 30.0%
! TCGACAGATGTTTATCTTTT+CGG - chr2.2:25497384-25497403 None:intergenic 30.0%
!!! AGTGATTTGGGTATTGATTT+TGG + chr2.2:25498785-25498804 MS.gene066204:CDS 30.0%
!!! GGGTGAATTTTTTTAGACAA+TGG + chr2.2:25498867-25498886 MS.gene066204:CDS 30.0%
AAAGTTCCAACAAGAACACT+AGG - chr2.2:25498032-25498051 None:intergenic 35.0%
AACCAATTTCTCTTCTGCTT+AGG - chr2.2:25499001-25499020 None:intergenic 35.0%
AAGCAGAAGAGAAATTGGTT+TGG + chr2.2:25499001-25499020 MS.gene066204:CDS 35.0%
AATGGAATCATTGATCTAGG+TGG + chr2.2:25497519-25497538 MS.gene066204:CDS 35.0%
AGGAGAAGAATCAAATCCAA+TGG + chr2.2:25498265-25498284 MS.gene066204:CDS 35.0%
CACAACATAGTAATGGTCAA+TGG + chr2.2:25498621-25498640 MS.gene066204:CDS 35.0%
CATGTAAAACCAATGTTTGG+TGG + chr2.2:25498422-25498441 MS.gene066204:CDS 35.0%
CATTGGATTCTATCAAGTGA+AGG - chr2.2:25497885-25497904 None:intergenic 35.0%
CCAATGGAAATTGATCCATT+AGG + chr2.2:25498281-25498300 MS.gene066204:CDS 35.0%
CTAAAAAAATTCACCCAACC+TGG - chr2.2:25498863-25498882 None:intergenic 35.0%
CTAATGGTTATAGCCCTTTA+GGG + chr2.2:25497832-25497851 MS.gene066204:CDS 35.0%
GCCTAATTTACCCCAAATTA+AGG + chr2.2:25498136-25498155 MS.gene066204:CDS 35.0%
GCTTGAATACCTCTATTGAT+TGG - chr2.2:25498008-25498027 None:intergenic 35.0%
GGTAAGATAGGTGAACATAT+TGG + chr2.2:25498533-25498552 MS.gene066204:CDS 35.0%
GTAAATGTTCCACCAAACAT+TGG - chr2.2:25498434-25498453 None:intergenic 35.0%
GTTGTTACAATATGCCCTAA+AGG - chr2.2:25497849-25497868 None:intergenic 35.0%
TCAAAGTGGAAATAAACCAG+TGG + chr2.2:25498667-25498686 MS.gene066204:CDS 35.0%
TCTTCACCTATCAACTCATT+GGG - chr2.2:25498965-25498984 None:intergenic 35.0%
TTAGGATGCAAGTACATGAA+TGG - chr2.2:25498182-25498201 None:intergenic 35.0%
TTCGGTCGATAATTTCTTGT+CGG - chr2.2:25497366-25497385 None:intergenic 35.0%
TTCTTCACCTATCAACTCAT+TGG - chr2.2:25498966-25498985 None:intergenic 35.0%
TTGTGCTAGTGCCTTAATTT+GGG - chr2.2:25498150-25498169 None:intergenic 35.0%
TTTGCATGTGTTTGTATAGG+TGG + chr2.2:25497486-25497505 MS.gene066204:intron 35.0%
! AAGCTTAAATGTGGTGTCAA+TGG - chr2.2:25497269-25497288 None:intergenic 35.0%
! AGCTTAAATGTGGTGTCAAT+GGG - chr2.2:25497268-25497287 None:intergenic 35.0%
! AGGTGAACATATTGGTGATT+GGG + chr2.2:25498541-25498560 MS.gene066204:CDS 35.0%
! CATGCTATTTATCCAAAAGC+TGG + chr2.2:25498710-25498729 MS.gene066204:CDS 35.0%
! CCTAATGGATCAATTTCCAT+TGG - chr2.2:25498284-25498303 None:intergenic 35.0%
! CTATAACCATTAGGTGCTAT+TGG - chr2.2:25497825-25497844 None:intergenic 35.0%
! GCCTTAATTTGGGGTAAATT+AGG - chr2.2:25498140-25498159 None:intergenic 35.0%
! TAGGTGAACATATTGGTGAT+TGG + chr2.2:25498540-25498559 MS.gene066204:CDS 35.0%
! TGGTGGAACATTTACTGATT+TGG + chr2.2:25498439-25498458 MS.gene066204:CDS 35.0%
! TGTTGTTTGGTTGACTATAG+TGG - chr2.2:25497668-25497687 None:intergenic 35.0%
!! AAATTGCCCAATGAGTTGAT+AGG + chr2.2:25498956-25498975 MS.gene066204:CDS 35.0%
!! AATGAGTTGATAGGTGAAGA+AGG + chr2.2:25498965-25498984 MS.gene066204:CDS 35.0%
!! AATTGTTTCTGGGGAGTATT+TGG + chr2.2:25498820-25498839 MS.gene066204:CDS 35.0%
!! ATGAGTTGATAGGTGAAGAA+GGG + chr2.2:25498966-25498985 MS.gene066204:CDS 35.0%
!! ATTGTTTCTGGGGAGTATTT+GGG + chr2.2:25498821-25498840 MS.gene066204:CDS 35.0%
!! GGTAATGGAATCATTGATCT+AGG + chr2.2:25497516-25497535 MS.gene066204:CDS 35.0%
!!! GGAACTTTTTTAGCACATGT+TGG + chr2.2:25498044-25498063 MS.gene066204:CDS 35.0%
AACATACCAACTTTGCCACA+AGG + chr2.2:25497295-25497314 MS.gene066204:CDS 40.0%
ACAACAAACCTCTCTTTGGT+TGG + chr2.2:25497682-25497701 MS.gene066204:CDS 40.0%
ATCAAATTGTTGAGCCAGGT+TGG + chr2.2:25498846-25498865 MS.gene066204:CDS 40.0%
CAACAAACCTCTCTTTGGTT+GGG + chr2.2:25497683-25497702 MS.gene066204:CDS 40.0%
CCAAACAACAAACCTCTCTT+TGG + chr2.2:25497678-25497697 MS.gene066204:CDS 40.0%
CCTAAAGGGCTATAACCATT+AGG - chr2.2:25497834-25497853 None:intergenic 40.0%
CCTAATGGTTATAGCCCTTT+AGG + chr2.2:25497831-25497850 MS.gene066204:CDS 40.0%
CCTAATGTGAAGAAACCTTG+AGG - chr2.2:25497645-25497664 None:intergenic 40.0%
CCTCAAGGTTTCTTCACATT+AGG + chr2.2:25497642-25497661 MS.gene066204:CDS 40.0%
CTTGTGCTAGTGCCTTAATT+TGG - chr2.2:25498151-25498170 None:intergenic 40.0%
GAAATCTGGACAACCTTAGA+AGG + chr2.2:25497573-25497592 MS.gene066204:CDS 40.0%
GACCTAAGCAGAAGAGAAAT+TGG + chr2.2:25498996-25499015 MS.gene066204:CDS 40.0%
GCAACTTTCTTTGAACCAAC+AGG + chr2.2:25497615-25497634 MS.gene066204:CDS 40.0%
GGAAATCAAATTGTTGAGCC+AGG + chr2.2:25498842-25498861 MS.gene066204:CDS 40.0%
GTTAGCTGGAAGCTTAAATG+TGG - chr2.2:25497278-25497297 None:intergenic 40.0%
GTTGCCAAAGATGAGTCAAA+TGG + chr2.2:25497711-25497730 MS.gene066204:CDS 40.0%
TCAAATTGTTGAGCCAGGTT+GGG + chr2.2:25498847-25498866 MS.gene066204:CDS 40.0%
TGAAAGAGATGAGTACCTTG+TGG - chr2.2:25497313-25497332 None:intergenic 40.0%
TGAGATCTGATCTCACACAT+TGG - chr2.2:25497902-25497921 None:intergenic 40.0%
TGAGGAAGATTTGAGCCTAA+TGG - chr2.2:25498299-25498318 None:intergenic 40.0%
TGCTCCATTTGACTCATCTT+TGG - chr2.2:25497718-25497737 None:intergenic 40.0%
TGGTTACCAATAGCACCTAA+TGG + chr2.2:25497816-25497835 MS.gene066204:CDS 40.0%
TTAGGCTCAAATCTTCCTCA+AGG + chr2.2:25498299-25498318 MS.gene066204:CDS 40.0%
! AAACCTTGAGGTATTCCTGT+TGG - chr2.2:25497633-25497652 None:intergenic 40.0%
! CAATGATGGTGCTTATTGGT+TGG + chr2.2:25498328-25498347 MS.gene066204:CDS 40.0%
! CTATCAAGTGAAGGCTTTTC+AGG - chr2.2:25497876-25497895 None:intergenic 40.0%
! CTTCAAGGTACTAGTGAGAT+TGG + chr2.2:25498740-25498759 MS.gene066204:CDS 40.0%
! GAAGCAATGATGGTGCTTAT+TGG + chr2.2:25498324-25498343 MS.gene066204:CDS 40.0%
! TGAAGAACCAATCCAGCTTT+TGG - chr2.2:25498725-25498744 None:intergenic 40.0%
! TGTGCTAGTGCCTTAATTTG+GGG - chr2.2:25498149-25498168 None:intergenic 40.0%
!! AAAGCTGGATTGGTTCTTCA+AGG + chr2.2:25498725-25498744 MS.gene066204:CDS 40.0%
!! ATAGGTGGTGGATTTGGTAA+TGG + chr2.2:25497501-25497520 MS.gene066204:intron 40.0%
!! CATTTACTGATTTGGCCATG+TGG + chr2.2:25498447-25498466 MS.gene066204:CDS 40.0%
!! CCAAAGAGAGGTTTGTTGTT+TGG - chr2.2:25497681-25497700 None:intergenic 40.0%
!! GGGGCATTGCTTTATAAGAA+AGG + chr2.2:25498245-25498264 MS.gene066204:CDS 40.0%
!! GTTTGTATAGGTGGTGGATT+TGG + chr2.2:25497495-25497514 MS.gene066204:intron 40.0%
!! TTTTACCCTTTTAATGGGCC+TGG + chr2.2:25498473-25498492 MS.gene066204:CDS 40.0%
AATGTGCCAGGCCCATTAAA+AGG - chr2.2:25498482-25498501 None:intergenic 45.0%
ACTAGAACCCAACCAAAGAG+AGG - chr2.2:25497693-25497712 None:intergenic 45.0%
ATCTGGACAACCTTAGAAGG+TGG + chr2.2:25497576-25497595 MS.gene066204:CDS 45.0%
ATGTGCCAGGCCCATTAAAA+GGG - chr2.2:25498481-25498500 None:intergenic 45.0%
CAAGCTCCTAGTGTTCTTGT+TGG + chr2.2:25498023-25498042 MS.gene066204:CDS 45.0%
GAACCAACAGGAATACCTCA+AGG + chr2.2:25497627-25497646 MS.gene066204:CDS 45.0%
GAAGGTGGACAAGATGACTT+AGG + chr2.2:25497591-25497610 MS.gene066204:CDS 45.0%
GCATGTGTTTGTATAGGTGG+TGG + chr2.2:25497489-25497508 MS.gene066204:intron 45.0%
GGCCTGGCACATTAAAAGTT+GGG + chr2.2:25498489-25498508 MS.gene066204:CDS 45.0%
GTCATCTTGTCCACCTTCTA+AGG - chr2.2:25497589-25497608 None:intergenic 45.0%
GTTGGAGAAGTTGGAGAAGT+TGG + chr2.2:25498922-25498941 MS.gene066204:CDS 45.0%
TCATTGGGCAATTTCAAGCC+AGG - chr2.2:25498950-25498969 None:intergenic 45.0%
TGAGTACCTTGTGGCAAAGT+TGG - chr2.2:25497304-25497323 None:intergenic 45.0%
! AAGTTGGAGAAGTTGGTTCC+TGG + chr2.2:25498929-25498948 MS.gene066204:CDS 45.0%
! AATGTGGTGTCAATGGGAAG+AGG - chr2.2:25497262-25497281 None:intergenic 45.0%
! AGCCCAACTTTTAATGTGCC+AGG - chr2.2:25498494-25498513 None:intergenic 45.0%
! AGGTGATGAGTTGGAGAAGT+TGG + chr2.2:25498913-25498932 MS.gene066204:CDS 45.0%
!! CAATGGGAAGAGGCTTTTTC+TGG - chr2.2:25497252-25497271 None:intergenic 45.0%
!! GGCAAAGTTGGTATGTTAGC+TGG - chr2.2:25497292-25497311 None:intergenic 45.0%
!!! AAGTGAAGGCTTTTCAGGTG+TGG - chr2.2:25497871-25497890 None:intergenic 45.0%
CCATCATTGCTTCCACCTTG+AGG - chr2.2:25498317-25498336 None:intergenic 50.0%
CCTCAAGGTGGAAGCAATGA+TGG + chr2.2:25498314-25498333 MS.gene066204:CDS 50.0%
CTCTTCTGCTTAGGTCCAGT+GGG - chr2.2:25498992-25499011 None:intergenic 50.0%
GGCTCAAATCTTCCTCAAGG+TGG + chr2.2:25498302-25498321 MS.gene066204:CDS 50.0%
GGGCCTGGCACATTAAAAGT+TGG + chr2.2:25498488-25498507 MS.gene066204:CDS 50.0%
TCTCTTCTGCTTAGGTCCAG+TGG - chr2.2:25498993-25499012 None:intergenic 50.0%
ATAGGTGAAGAAGGGCCCAC+TGG + chr2.2:25498974-25498993 MS.gene066204:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 25497223 25499036 25497223 ID=MS.gene066204
chr2.2 mRNA 25497223 25499036 25497223 ID=MS.gene066204.t1;Parent=MS.gene066204
chr2.2 exon 25497223 25497316 25497223 ID=MS.gene066204.t1.exon1;Parent=MS.gene066204.t1
chr2.2 CDS 25497223 25497316 25497223 ID=cds.MS.gene066204.t1;Parent=MS.gene066204.t1
chr2.2 exon 25497505 25499036 25497505 ID=MS.gene066204.t1.exon2;Parent=MS.gene066204.t1
chr2.2 CDS 25497505 25499036 25497505 ID=cds.MS.gene066204.t1;Parent=MS.gene066204.t1
Gene Sequence

>MS.gene066204

ATGGGGAACAATTCATCTTCTTCTAACCAGAAAAAGCCTCTTCCCATTGACACCACATTTAAGCTTCCAGCTAACATACCAACTTTGCCACAAGGTACTCATCTCTTTCACTTCCTTCTTGATAGTATTTTCACCAAATTCCGACAAGAAATTATCGACCGAAAAGATAAACATCTGTCGATAATTCTCTTGTCACTAATTAAATTTGTTCTAGTAGTGATAATTGTGTCATTCTAATTTCTAGTATTTTTTTTAATGATAACTTTGCATGTGTTTGTATAGGTGGTGGATTTGGTAATGGAATCATTGATCTAGGTGGCCTAAAAGTGTCACAAATATCAACATTCAACGAAATCTGGACAACCTTAGAAGGTGGACAAGATGACTTAGGAGCAACTTTCTTTGAACCAACAGGAATACCTCAAGGTTTCTTCACATTAGGCCACTATAGTCAACCAAACAACAAACCTCTCTTTGGTTGGGTTCTAGTTGCCAAAGATGAGTCAAATGGAGCATTGAAAAAACCAATTGATTACACACTTGTTTGGAGTAGCAAGTCAGAAAAAATCAAACAAGATAAAGATGGTTACATTTGGTTACCAATAGCACCTAATGGTTATAGCCCTTTAGGGCATATTGTAACAACCACACCTGAAAAGCCTTCACTTGATAGAATCCAATGTGTGAGATCAGATCTCACTGACCAATGTGAGATTAATTCATGGATTTGGGGTAAAGATAAAAAAATTGATGAAAAAGGTTTCAATGTTCATTATGTTAGACCAATCAATAGAGGTATTCAAGCTCCTAGTGTTCTTGTTGGAACTTTTTTAGCACATGTTGGTGAAATTACAAATAGCCCTCTTCCTATTTCTTGTTTGAAAAATCTCAACTTTATGAATTTTTCATCAATGCCTAATTTACCCCAAATTAAGGCACTAGCACAAGCTTATTCTCCATTCATGTACTTGCATCCTAATGAAAAGTTTCAACCATGTTCAATCAAATGGTATTTCACAAATGGGGCATTGCTTTATAAGAAAGGAGAAGAATCAAATCCAATGGAAATTGATCCATTAGGCTCAAATCTTCCTCAAGGTGGAAGCAATGATGGTGCTTATTGGTTGGATCTTCCAAAAGATAAAGCTAATAGAGAAAGAGTCAAAAAAGGTGATTTCAAAAATTGTCAAGGTTATTTTCATGTAAAACCAATGTTTGGTGGAACATTTACTGATTTGGCCATGTGGATTTTTTACCCTTTTAATGGGCCTGGCACATTAAAAGTTGGGCTCATAGATAACATTTCACTTGGTAAGATAGGTGAACATATTGGTGATTGGGAGCATGTGACTTTAAGAGTAAGTAACTTCAATGGAGAGTTAAAAAGTGTTTATTTTTCACAACATAGTAATGGTCAATGGGTTGATGCTTCAGAAGTTGAATTTCAAAGTGGAAATAAACCAGTGGCTTATTCTTCATTAAATGGTCATGCTATTTATCCAAAAGCTGGATTGGTTCTTCAAGGTACTAGTGAGATTGGAATAAAGAATGAAACTAAGAAGAGTGATTTGGGTATTGATTTTGGAGTTAATTTTGAAATTGTTTCTGGGGAGTATTTGGGAAATCAAATTGTTGAGCCAGGTTGGGTGAATTTTTTTAGACAATGGGGTCCTAAAATTACTTATAATTTAGGTGATGAGTTGGAGAAGTTGGAGAAGTTGGTTCCTGGCTTGAAATTGCCCAATGAGTTGATAGGTGAAGAAGGGCCCACTGGACCTAAGCAGAAGAGAAATTGGTTTGGTGATGAAATTTGA
Protein sequence

>MS.gene066204.t1

MGNNSSSSNQKKPLPIDTTFKLPANIPTLPQGGGFGNGIIDLGGLKVSQISTFNEIWTTLEGGQDDLGATFFEPTGIPQGFFTLGHYSQPNNKPLFGWVLVAKDESNGALKKPIDYTLVWSSKSEKIKQDKDGYIWLPIAPNGYSPLGHIVTTTPEKPSLDRIQCVRSDLTDQCEINSWIWGKDKKIDEKGFNVHYVRPINRGIQAPSVLVGTFLAHVGEITNSPLPISCLKNLNFMNFSSMPNLPQIKALAQAYSPFMYLHPNEKFQPCSIKWYFTNGALLYKKGEESNPMEIDPLGSNLPQGGSNDGAYWLDLPKDKANRERVKKGDFKNCQGYFHVKPMFGGTFTDLAMWIFYPFNGPGTLKVGLIDNISLGKIGEHIGDWEHVTLRVSNFNGELKSVYFSQHSNGQWVDASEVEFQSGNKPVAYSSLNGHAIYPKAGLVLQGTSEIGIKNETKKSDLGIDFGVNFEIVSGEYLGNQIVEPGWVNFFRQWGPKITYNLGDELEKLEKLVPGLKLPNELIGEEGPTGPKQKRNWFGDEI