AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066277

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066277.t1 MTR_8g038745 55.882 68 30 0 22 89 1 68 1.02e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCATTTCGAGATGATTTC+GGG 0.227385 8.3:-68328292 MS.gene066277:CDS
GAATATGGAACCAATGTTTC+AGG 0.255948 8.3:-68328818 MS.gene066277:CDS
TACTGTAATGGTTGTGTTCC+TGG 0.263116 8.3:-68329220 MS.gene066277:CDS
ACAAATACGCAAATAGTAAA+AGG 0.269504 8.3:-68329348 MS.gene066277:CDS
GCATCATTTCGAGATGATTT+CGG 0.275502 8.3:-68328293 MS.gene066277:CDS
TTTACAGTGTGGCAATGTTC+TGG 0.292487 8.3:-68328363 MS.gene066277:intron
AGAGGTACAGACAATGTTAT+TGG 0.332752 8.3:-68329262 MS.gene066277:CDS
TGGTCATGACCGTAAGATTA+AGG 0.341101 8.3:-68329287 MS.gene066277:CDS
TTTCTTGAACATCCTAGTTC+AGG 0.346685 8.3:-68328326 MS.gene066277:CDS
GTTCGAACCTTGGGACTTAC+CGG 0.351183 8.3:-68329504 MS.gene066277:CDS
GACATGAAGGTGGCTGGTCT+TGG 0.374690 8.3:+68328155 None:intergenic
CATATCGATGTGTCCACTTT+CGG 0.387067 8.3:+68328693 None:intergenic
TTCCTGGTGTTAAAAGCAAC+TGG 0.389751 8.3:-68329204 MS.gene066277:CDS
TGGCTGGTCTTGGATGATAC+TGG 0.427061 8.3:+68328165 None:intergenic
GAAGGACACAGTGTGGATAC+TGG 0.428296 8.3:-68329318 MS.gene066277:CDS
TCGTTGAGAATGATCTACTA+TGG 0.438845 8.3:-68328546 MS.gene066277:CDS
TGTTTGCAAGTTCGAACCTT+GGG 0.438935 8.3:-68329513 MS.gene066277:CDS
TGATGAGGTTCCTGAAACAT+TGG 0.440724 8.3:+68328808 None:intergenic
AATGTTTCAGGAACCTCATC+AGG 0.458637 8.3:-68328806 MS.gene066277:CDS
TCGGAACTCATGTGTTTACT+TGG 0.464802 8.3:+68328963 None:intergenic
TCCTCGTCAATAGTTTCAAA+CGG 0.474217 8.3:+68328664 None:intergenic
GACCGTAAGATTAAGGTTAG+AGG 0.484303 8.3:-68329280 MS.gene066277:CDS
GAAAATCAAGAAGCAGTTGA+AGG 0.489171 8.3:-68328936 MS.gene066277:CDS
TACCTCTAACCTTAATCTTA+CGG 0.491526 8.3:+68329278 None:intergenic
TTCAATCTCTGAAAATGAGA+TGG 0.499943 8.3:-68328749 MS.gene066277:CDS
GCATAGTATTCTCCTGAACT+AGG 0.503449 8.3:+68328314 None:intergenic
GAAGACTGGTATGTTGAGAA+AGG 0.511939 8.3:+68328769 None:intergenic
TTGTTTCTTCGTAGAGTGGA+AGG 0.518122 8.3:+68328191 None:intergenic
AAAACACTTGTGTACTGTAA+TGG 0.519556 8.3:-68329232 MS.gene066277:CDS
GATAGAATGATATGTGATGA+AGG 0.532647 8.3:-68328990 MS.gene066277:CDS
GAAGTATCTTTATCGAACTC+AGG 0.533769 8.3:-68328727 MS.gene066277:CDS
TAGAATGATATGTGATGAAG+GGG 0.540119 8.3:-68328988 MS.gene066277:CDS
TTCGAACCTTGGGACTTACC+GGG 0.549304 8.3:-68329503 MS.gene066277:intron
ATAGAATGATATGTGATGAA+GGG 0.555622 8.3:-68328989 MS.gene066277:CDS
ACAAACCTGGTTATCATCAA+AGG 0.564326 8.3:+68329156 None:intergenic
CTCGACTGCAGATAAACCTC+GGG 0.565331 8.3:-68328246 MS.gene066277:CDS
TACCACCTTTGATGATAACC+AGG 0.567592 8.3:-68329161 MS.gene066277:intron
ATCAATTCGATGTTAAATGA+CGG 0.567730 8.3:-68328631 MS.gene066277:CDS
ATGTTTGCAAGTTCGAACCT+TGG 0.568862 8.3:-68329514 MS.gene066277:CDS
ATAGTAAAAGGTTTAACAGA+AGG 0.579914 8.3:-68329336 MS.gene066277:CDS
CAATGTTCTGGATACTTCAA+CGG 0.597908 8.3:-68328351 MS.gene066277:CDS
ATCTGCAGTCGAGTCACAAG+AGG 0.603959 8.3:+68328256 None:intergenic
TGTGGATACTGGAAAGCCAC+TGG 0.617140 8.3:-68329307 MS.gene066277:CDS
AATCTTACGGTCATGACCAG+TGG 0.621150 8.3:+68329291 None:intergenic
ACTCGACTGCAGATAAACCT+CGG 0.628338 8.3:-68328247 MS.gene066277:CDS
TCCGTTTGAAACTATTGACG+AGG 0.648885 8.3:-68328665 MS.gene066277:CDS
TAAAATCCGATGATCCCGAA+TGG 0.649145 8.3:-68329393 MS.gene066277:CDS
ATCATTTCGAGATGATTTCG+GGG 0.650121 8.3:-68328291 MS.gene066277:CDS
AACCTGGTTATCATCAAAGG+TGG 0.663139 8.3:+68329159 None:intergenic
GAATGATATGTGATGAAGGG+GGG 0.665909 8.3:-68328986 MS.gene066277:CDS
TCCTGGTGTTAAAAGCAACT+GGG 0.666261 8.3:-68329203 MS.gene066277:CDS
TCATTTCGAGATGATTTCGG+GGG 0.671935 8.3:-68328290 MS.gene066277:CDS
GTAGATCATTCTCAACGACT+CGG 0.673640 8.3:+68328551 None:intergenic
TTTGCGTATTTGTAATCACG+AGG 0.676971 8.3:+68329358 None:intergenic
ACACAATTGATCATCTCCCG+AGG 0.677154 8.3:+68328230 None:intergenic
TTTAACAGAAGGACACAGTG+TGG 0.694289 8.3:-68329325 MS.gene066277:CDS
AGAATGATATGTGATGAAGG+GGG 0.758539 8.3:-68328987 MS.gene066277:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTATCAAAGAAAATATTTA+CGG + chr8.3:68329159-68329178 None:intergenic 15.0%
!!! TTTTATGAGAAAATAATGAT+GGG + chr8.3:68328184-68328203 None:intergenic 15.0%
!!! TTTTTATGAGAAAATAATGA+TGG + chr8.3:68328185-68328204 None:intergenic 15.0%
!! GTATCAAAGAAAATATTTAC+GGG + chr8.3:68329158-68329177 None:intergenic 20.0%
!! TTTATGAGAAAATAATGATG+GGG + chr8.3:68328183-68328202 None:intergenic 20.0%
!!! ATGTTTTAACTTTATGAGTA+TGG - chr8.3:68328523-68328542 MS.gene066277:CDS 20.0%
! ACAAATACGCAAATAGTAAA+AGG - chr8.3:68328296-68328315 MS.gene066277:CDS 25.0%
! ATAGAATGATATGTGATGAA+GGG - chr8.3:68328655-68328674 MS.gene066277:CDS 25.0%
! ATAGTAAAAGGTTTAACAGA+AGG - chr8.3:68328308-68328327 MS.gene066277:CDS 25.0%
! ATCAATTCGATGTTAAATGA+CGG - chr8.3:68329013-68329032 MS.gene066277:CDS 25.0%
!! AAATAATGATGGGGTTTATT+TGG + chr8.3:68328174-68328193 None:intergenic 25.0%
!!! AGGATAATCAACATTTTTTC+CGG + chr8.3:68328560-68328579 None:intergenic 25.0%
!!! TCTTCTAAAATTTTACAGTG+TGG - chr8.3:68329270-68329289 MS.gene066277:CDS 25.0%
AAAACACTTGTGTACTGTAA+TGG - chr8.3:68328412-68328431 MS.gene066277:intron 30.0%
AAGCATGTTTCATGTAAGAA+AGG + chr8.3:68328805-68328824 None:intergenic 30.0%
AGGACTAAAGAAAAACCATT+CGG + chr8.3:68328269-68328288 None:intergenic 30.0%
CATGAAACATGCTTAGAATA+TGG - chr8.3:68328811-68328830 MS.gene066277:CDS 30.0%
CATTCTAGTAGCATTTCAAA+AGG + chr8.3:68329230-68329249 None:intergenic 30.0%
GATAGAATGATATGTGATGA+AGG - chr8.3:68328654-68328673 MS.gene066277:CDS 30.0%
TACCTCTAACCTTAATCTTA+CGG + chr8.3:68328369-68328388 None:intergenic 30.0%
TAGAATGATATGTGATGAAG+GGG - chr8.3:68328656-68328675 MS.gene066277:CDS 30.0%
TTGAAGGAATTCTTGATGTA+AGG - chr8.3:68328724-68328743 MS.gene066277:CDS 30.0%
TTGTTGTAACAAAACAAACC+TGG + chr8.3:68328504-68328523 None:intergenic 30.0%
! AAAAGAAGACACACTTTTGT+CGG - chr8.3:68329055-68329074 MS.gene066277:intron 30.0%
! AAAAGTCCTCTACGAAATTT+TGG + chr8.3:68328583-68328602 None:intergenic 30.0%
! TTCAATCTCTGAAAATGAGA+TGG - chr8.3:68328895-68328914 MS.gene066277:intron 30.0%
!! GTTTTATTCATACTGTGATG+TGG - chr8.3:68329191-68329210 MS.gene066277:CDS 30.0%
!!! CTTTTGAAATGCTACTAGAA+TGG - chr8.3:68329228-68329247 MS.gene066277:CDS 30.0%
!!! GGTTTATTTGGTTTTTTACC+CGG + chr8.3:68328162-68328181 None:intergenic 30.0%
!!! TGAGAAAGGAAAGTACTTTT+CGG + chr8.3:68328864-68328883 None:intergenic 30.0%
ACAAACCTGGTTATCATCAA+AGG + chr8.3:68328491-68328510 None:intergenic 35.0%
AGAATGATATGTGATGAAGG+GGG - chr8.3:68328657-68328676 MS.gene066277:CDS 35.0%
ATCATTTCGAGATGATTTCG+GGG - chr8.3:68329353-68329372 MS.gene066277:CDS 35.0%
CATCATTTCGAGATGATTTC+GGG - chr8.3:68329352-68329371 MS.gene066277:CDS 35.0%
GAAAATCAAGAAGCAGTTGA+AGG - chr8.3:68328708-68328727 MS.gene066277:CDS 35.0%
GAATATGGAACCAATGTTTC+AGG - chr8.3:68328826-68328845 MS.gene066277:intron 35.0%
GCATCATTTCGAGATGATTT+CGG - chr8.3:68329351-68329370 MS.gene066277:CDS 35.0%
GGACTAAAGAAAAACCATTC+GGG + chr8.3:68328268-68328287 None:intergenic 35.0%
TATCCTCCAAAATTTCGTAG+AGG - chr8.3:68328574-68328593 MS.gene066277:intron 35.0%
TCCTCGTCAATAGTTTCAAA+CGG + chr8.3:68328983-68329002 None:intergenic 35.0%
TCGTTGAGAATGATCTACTA+TGG - chr8.3:68329098-68329117 MS.gene066277:intron 35.0%
TTTCTTGAACATCCTAGTTC+AGG - chr8.3:68329318-68329337 MS.gene066277:CDS 35.0%
TTTGCGTATTTGTAATCACG+AGG + chr8.3:68328289-68328308 None:intergenic 35.0%
! AGAGGTACAGACAATGTTAT+TGG - chr8.3:68328382-68328401 MS.gene066277:intron 35.0%
! CAATGTTCTGGATACTTCAA+CGG - chr8.3:68329293-68329312 MS.gene066277:CDS 35.0%
! CATTTTCAGAGATTGAAGAC+TGG + chr8.3:68328892-68328911 None:intergenic 35.0%
! GAAGTATCTTTATCGAACTC+AGG - chr8.3:68328917-68328936 MS.gene066277:intron 35.0%
! GAGTATGGTATGAGTTTTTC+CGG - chr8.3:68328538-68328557 MS.gene066277:CDS 35.0%
! GGATTTTATGATTCCGAAAG+TGG - chr8.3:68328938-68328957 MS.gene066277:CDS 35.0%
!! GTTTTTGTTTCTTCGTAGAG+TGG + chr8.3:68329460-68329479 None:intergenic 35.0%
!!! TTCCTCTTCAGCTTTTTTCT+CGG + chr8.3:68328631-68328650 None:intergenic 35.0%
AACCTGGTTATCATCAAAGG+TGG + chr8.3:68328488-68328507 None:intergenic 40.0%
AATGTTTCAGGAACCTCATC+AGG - chr8.3:68328838-68328857 MS.gene066277:intron 40.0%
ATGTTTGCAAGTTCGAACCT+TGG - chr8.3:68328130-68328149 MS.gene066277:CDS 40.0%
CATATCGATGTGTCCACTTT+CGG + chr8.3:68328954-68328973 None:intergenic 40.0%
GAAAAACCATTCGGGATCAT+CGG + chr8.3:68328260-68328279 None:intergenic 40.0%
GAAGACTGGTATGTTGAGAA+AGG + chr8.3:68328878-68328897 None:intergenic 40.0%
GAATGATATGTGATGAAGGG+GGG - chr8.3:68328658-68328677 MS.gene066277:CDS 40.0%
GACCGTAAGATTAAGGTTAG+AGG - chr8.3:68328364-68328383 MS.gene066277:intron 40.0%
GCATAGTATTCTCCTGAACT+AGG + chr8.3:68329333-68329352 None:intergenic 40.0%
GTAGATCATTCTCAACGACT+CGG + chr8.3:68329096-68329115 None:intergenic 40.0%
TAAAATCCGATGATCCCGAA+TGG - chr8.3:68328251-68328270 MS.gene066277:CDS 40.0%
TACTGTAATGGTTGTGTTCC+TGG - chr8.3:68328424-68328443 MS.gene066277:intron 40.0%
TCATTTCGAGATGATTTCGG+GGG - chr8.3:68329354-68329373 MS.gene066277:CDS 40.0%
TCGGAACTCATGTGTTTACT+TGG + chr8.3:68328684-68328703 None:intergenic 40.0%
TGATGAGGTTCCTGAAACAT+TGG + chr8.3:68328839-68328858 None:intergenic 40.0%
TGGTCATGACCGTAAGATTA+AGG - chr8.3:68328357-68328376 MS.gene066277:CDS 40.0%
TGTTTGCAAGTTCGAACCTT+GGG - chr8.3:68328131-68328150 MS.gene066277:CDS 40.0%
TTTAACAGAAGGACACAGTG+TGG - chr8.3:68328319-68328338 MS.gene066277:CDS 40.0%
TTTACAGTGTGGCAATGTTC+TGG - chr8.3:68329281-68329300 MS.gene066277:CDS 40.0%
! AGCAGCTCTTTTTGACATGA+AGG + chr8.3:68329505-68329524 None:intergenic 40.0%
! AGCTCTTTTTGACATGAAGG+TGG + chr8.3:68329502-68329521 None:intergenic 40.0%
! AGTCCTCTACGAAATTTTGG+AGG + chr8.3:68328580-68328599 None:intergenic 40.0%
! TACCACCTTTGATGATAACC+AGG - chr8.3:68328483-68328502 MS.gene066277:intron 40.0%
! TCCGTTTGAAACTATTGACG+AGG - chr8.3:68328979-68328998 MS.gene066277:CDS 40.0%
! TCCTGGTGTTAAAAGCAACT+GGG - chr8.3:68328441-68328460 MS.gene066277:intron 40.0%
! TGCTTCTTGATTTTCGCGAT+CGG + chr8.3:68328703-68328722 None:intergenic 40.0%
! TTCCTGGTGTTAAAAGCAAC+TGG - chr8.3:68328440-68328459 MS.gene066277:intron 40.0%
! TTGTTTCTTCGTAGAGTGGA+AGG + chr8.3:68329456-68329475 None:intergenic 40.0%
AATCTTACGGTCATGACCAG+TGG + chr8.3:68328356-68328375 None:intergenic 45.0%
ACACAATTGATCATCTCCCG+AGG + chr8.3:68329417-68329436 None:intergenic 45.0%
ACTCGACTGCAGATAAACCT+CGG - chr8.3:68329397-68329416 MS.gene066277:CDS 45.0%
AGATGATTTCGGGGGATTTG+AGG - chr8.3:68329362-68329381 MS.gene066277:CDS 45.0%
AGCCGAGAAAAAAGCTGAAG+AGG - chr8.3:68328626-68328645 MS.gene066277:CDS 45.0%
! AAGTACTTTTCGGCCTGATG+AGG + chr8.3:68328854-68328873 None:intergenic 45.0%
! CTTTTTGACATGAAGGTGGC+TGG + chr8.3:68329498-68329517 None:intergenic 45.0%
! TTTTTACCCGGTAAGTCCCA+AGG + chr8.3:68328150-68328169 None:intergenic 45.0%
!!! ACCCAGTTGCTTTTAACACC+AGG + chr8.3:68328445-68328464 None:intergenic 45.0%
ATCTGCAGTCGAGTCACAAG+AGG + chr8.3:68329391-68329410 None:intergenic 50.0%
CTCGACTGCAGATAAACCTC+GGG - chr8.3:68329398-68329417 MS.gene066277:CDS 50.0%
GAAGGACACAGTGTGGATAC+TGG - chr8.3:68328326-68328345 MS.gene066277:CDS 50.0%
GTTCGAACCTTGGGACTTAC+CGG - chr8.3:68328140-68328159 MS.gene066277:CDS 50.0%
TGTGGATACTGGAAAGCCAC+TGG - chr8.3:68328337-68328356 MS.gene066277:CDS 50.0%
TTCGAACCTTGGGACTTACC+GGG - chr8.3:68328141-68328160 MS.gene066277:CDS 50.0%
! TCATTCTCAACGACTCGGAC+TGG + chr8.3:68329091-68329110 None:intergenic 50.0%
!! TGGCTGGTCTTGGATGATAC+TGG + chr8.3:68329482-68329501 None:intergenic 50.0%
!! GACATGAAGGTGGCTGGTCT+TGG + chr8.3:68329492-68329511 None:intergenic 55.0%
! CTCAACGACTCGGACTGGAC+AGG + chr8.3:68329086-68329105 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 68328121 68329545 68328121 ID=MS.gene066277
chr8.3 mRNA 68328121 68329545 68328121 ID=MS.gene066277.t1;Parent=MS.gene066277
chr8.3 exon 68329504 68329545 68329504 ID=MS.gene066277.t1.exon1;Parent=MS.gene066277.t1
chr8.3 CDS 68329504 68329545 68329504 ID=cds.MS.gene066277.t1;Parent=MS.gene066277.t1
chr8.3 exon 68329162 68329427 68329162 ID=MS.gene066277.t1.exon2;Parent=MS.gene066277.t1
chr8.3 CDS 68329162 68329427 68329162 ID=cds.MS.gene066277.t1;Parent=MS.gene066277.t1
chr8.3 exon 68328926 68329072 68328926 ID=MS.gene066277.t1.exon3;Parent=MS.gene066277.t1
chr8.3 CDS 68328926 68329072 68328926 ID=cds.MS.gene066277.t1;Parent=MS.gene066277.t1
chr8.3 exon 68328624 68328839 68328624 ID=MS.gene066277.t1.exon4;Parent=MS.gene066277.t1
chr8.3 CDS 68328624 68328839 68328624 ID=cds.MS.gene066277.t1;Parent=MS.gene066277.t1
chr8.3 exon 68328509 68328574 68328509 ID=MS.gene066277.t1.exon5;Parent=MS.gene066277.t1
chr8.3 CDS 68328509 68328574 68328509 ID=cds.MS.gene066277.t1;Parent=MS.gene066277.t1
chr8.3 exon 68328121 68328378 68328121 ID=MS.gene066277.t1.exon6;Parent=MS.gene066277.t1
chr8.3 CDS 68328121 68328378 68328121 ID=cds.MS.gene066277.t1;Parent=MS.gene066277.t1
Gene Sequence

>MS.gene066277

CTGAAGTCGATGTTTGCAAGTTCGAACCTTGGGACTTACCGGGTAAAAAACCAAATAAACCCCATCATTATTTTCTCATAAAAATGCAATCTTTTTAATGTTTTTGATACTTTTTCAGCTTGTTCAATCATAAAATCCGATGATCCCGAATGGTTTTTCTTTAGTCCTCGTGATTACAAATACGCAAATAGTAAAAGGTTTAACAGAAGGACACAGTGTGGATACTGGAAAGCCACTGGTCATGACCGTAAGATTAAGGTTAGAGGTACAGACAATGTTATTGGAACAAAAAAAACACTTGTGTACTGTAATGGTTGTGTTCCTGGTGTTAAAAGCAACTGGGTGATTCATGAATATCATTCTACCACCTTTGATGATAACCAGGTTTGTTTTGTTACAACAATGTTTTAACTTTATGAGTATGGTATGAGTTTTTCCGGAAAAAATGTTGATTATCCTCCAAAATTTCGTAGAGGACTTTTGTTTTGTGTCGCTTGATGAAGAAAGCCGAGAAAAAAGCTGAAGAGGAAACTGATAGAATGATATGTGATGAAGGGGGGCCAAGTAAACACATGAGTTCCGATCGCGAAAATCAAGAAGCAGTTGAAGGAATTCTTGATGTAAGGATTTCATTCTTGATAAATGACAACAGAAATTTTGAGAACAAAGTCTTAATTTAAACCTTTCTTACATGAAACATGCTTAGAATATGGAACCAATGTTTCAGGAACCTCATCAGGCCGAAAAGTACTTTCCTTTCTCAACATACCAGTCTTCAATCTCTGAAAATGAGATGGAAGTATCTTTATCGAACTCAGGATTTTATGATTCCGAAAGTGGACACATCGATATGCAGAGTCCGTTTGAAACTATTGACGAGGAAAATCAGTTCATCAATTCGATGTTAAATGACGGATATCTTGTTAATAGCGAAAAAAGAAGACACACTTTTGTCGGCTTGTCCTGTCCAGTCCGAGTCGTTGAGAATGATCTACTATGGAAGCAGTGACACAGATGTTGAATTAGTCTCTACGCCCGTAAATATTTTCTTTGATACATTCAGAAGTTCTGTTTTATTCATACTGTGATGTGGTTGTTTTTCTCTTCCTTTTGAAATGCTACTAGAATGGAATTTCATATTGTTTATTTTCTTCTAAAATTTTACAGTGTGGCAATGTTCTGGATACTTCAACGGCATTTCTTGAACATCCTAGTTCAGGAGAATACTATGCATCATTTCGAGATGATTTCGGGGGATTTGAGGCATCCTCTTGTGACTCGACTGCAGATAAACCTCGGGAGATGATCAATTGTGTTGAAATTTCTAGTGCACCTTCCACTCTACGAAGAAACAAAAACCAGTATCATCCAAGACCAGCCACCTTCATGTCAAAAAGAGCTGCTTCAAGACGATCTCAAACATAG
Protein sequence

>MS.gene066277.t1

LKSMFASSNLGTYRLVQSUNPMIPNGFSLVLVITNTQIVKGLTEGHSVDTGKPLVMTVRLRLEVQTMLLEQKKHLCTVMVVFLVLKATGUFMNIILPPLMITRGLLFCVAUURKPRKKLKRKLIEUYVMKGGQVNTUVPIAKIKKQLKEFLIIWNQCFRNLIRPKSTFLSQHTSLQSLKMRWKYLYRTQDFMIPKVDTSICRVRLKLLTRKISSSIRCUMTDIFPSRUEUSTMEAVTQMLNUSLRPVAMFWILQRHFLNILVQENTMHHFEMISGDLRHPLVTRLQINLGRUSIVLKFLVHLPLYEETKTSIIQDQPPSCQKELLQDDLKH