Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066463.t1 | XP_003629040.1 | 75.4 | 179 | 44 | 0 | 1 | 179 | 1 | 179 | 1.20E-64 | 256.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066463.t1 | G7L7W4 | 75.4 | 179 | 44 | 0 | 1 | 179 | 1 | 179 | 8.4e-65 | 256.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene066463 | 0.811893 | 5.57E-51 | -1.69E-46 |
| MS.gene050826 | MS.gene066463 | 0.805272 | 1.45E-49 | -1.69E-46 |
| MS.gene053563 | MS.gene066463 | 0.804712 | 1.89E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene066463 | 0.800747 | 1.25E-48 | -1.69E-46 |
| MS.gene059439 | MS.gene066463 | 0.811636 | 6.34E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066463.t1 | MTR_8g072450 | 93.296 | 179 | 12 | 0 | 1 | 179 | 1 | 179 | 3.62e-99 | 284 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGCTAAAGTGGTTGAAAT+TGG | 0.250565 | 8.3:-29982513 | MS.gene066463:CDS |
| GCTAGAAAATCGAGTAATTC+AGG | 0.254707 | 8.3:-29983276 | MS.gene066463:intron |
| ATTGGATCGTCTTGAGCTTC+TGG | 0.310419 | 8.3:-29983616 | MS.gene066463:intron |
| ATTGGATGCAAATGAGAATA+TGG | 0.320746 | 8.3:-29982763 | MS.gene066463:CDS |
| ATAGCTGGAAAGCTAGAAAA+AGG | 0.330571 | 8.3:-29982795 | MS.gene066463:CDS |
| CATTAAACTCACCATCTTGC+AGG | 0.336387 | 8.3:-29982580 | MS.gene066463:CDS |
| AATCAAAGCAATGTCTAGAA+TGG | 0.406480 | 8.3:-29982549 | MS.gene066463:CDS |
| TGTTGCCATTCACAAATGTT+TGG | 0.407820 | 8.3:-29983381 | MS.gene066463:CDS |
| TGCAAATGAGAATATGGATA+TGG | 0.412766 | 8.3:-29982757 | MS.gene066463:CDS |
| TCACCACAAAGGCACGTTGC+TGG | 0.417918 | 8.3:-29983312 | MS.gene066463:CDS |
| TGTCTAGAATGGGTTACAAT+AGG | 0.431528 | 8.3:-29982538 | MS.gene066463:CDS |
| TTGTTCCAAACATTTGTGAA+TGG | 0.448955 | 8.3:+29983376 | None:intergenic |
| GATCCAGCAACGTGCCTTTG+TGG | 0.459396 | 8.3:+29983309 | None:intergenic |
| GAATGGGTTACAATAGGAAA+TGG | 0.469355 | 8.3:-29982532 | MS.gene066463:CDS |
| TCTTCCACATCTATAATAGC+TGG | 0.475039 | 8.3:-29982810 | MS.gene066463:CDS |
| TGGCTAAAGTGGTTGAAATT+GGG | 0.480310 | 8.3:-29982512 | MS.gene066463:CDS |
| ATCAAAGCAATGTCTAGAAT+GGG | 0.486283 | 8.3:-29982548 | MS.gene066463:CDS |
| GTAGGATAGCATTGTTGTGG+AGG | 0.497964 | 8.3:-29982609 | MS.gene066463:intron |
| TCTTCAGCTGAGTATTGACT+TGG | 0.499669 | 8.3:-29982859 | MS.gene066463:intron |
| ACAATAGGAAATGGCTAAAG+TGG | 0.506453 | 8.3:-29982523 | MS.gene066463:CDS |
| CTTGTCGATTCTCCAACGAT+TGG | 0.532394 | 8.3:-29983634 | MS.gene066463:CDS |
| CAAACACAGATGAAAACAAA+GGG | 0.536216 | 8.3:-29983671 | MS.gene066463:CDS |
| CTGAGTATTGACTTGGACAT+GGG | 0.547034 | 8.3:-29982852 | MS.gene066463:CDS |
| CTTTCCAGCTATTATAGATG+TGG | 0.555271 | 8.3:+29982806 | None:intergenic |
| GCTCAAGACGATCCAATCGT+TGG | 0.572417 | 8.3:+29983622 | None:intergenic |
| ATTAAACTCACCATCTTGCA+GGG | 0.587021 | 8.3:-29982579 | MS.gene066463:CDS |
| AAAGTTACTTGTGTTATGAG+AGG | 0.587359 | 8.3:+29982708 | None:intergenic |
| ATTCTATGTGCCCTGCAAGA+TGG | 0.593541 | 8.3:+29982569 | None:intergenic |
| ACAAACACAGATGAAAACAA+AGG | 0.596759 | 8.3:-29983672 | MS.gene066463:CDS |
| GCTGAGTATTGACTTGGACA+TGG | 0.601595 | 8.3:-29982853 | MS.gene066463:CDS |
| CAATCGTTGGAGAATCGACA+AGG | 0.612069 | 8.3:+29983635 | None:intergenic |
| TTTGAAGAGGTTCACCACAA+AGG | 0.635515 | 8.3:-29983323 | MS.gene066463:CDS |
| CTTGTAGGATAGCATTGTTG+TGG | 0.638221 | 8.3:-29982612 | MS.gene066463:intron |
| TGGACATGGGAAACACATCA+AGG | 0.640707 | 8.3:-29982839 | MS.gene066463:CDS |
| AACAAGAACAACAATGCAAG+AGG | 0.640851 | 8.3:-29983358 | MS.gene066463:CDS |
| AATCGTTGGAGAATCGACAA+GGG | 0.726740 | 8.3:+29983636 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAAAAAATAAAATTATG+TGG | + | chr8.3:29982941-29982960 | None:intergenic | 10.0% |
| !! | CTATTAGTAAAAAAATTATA+TGG | + | chr8.3:29983535-29983554 | None:intergenic | 10.0% |
| !! | TTTAATTAATGACCATTATA+TGG | + | chr8.3:29982715-29982734 | None:intergenic | 15.0% |
| !!! | AAAAGTATACATTTTTCTAA+AGG | - | chr8.3:29983096-29983115 | MS.gene066463:intron | 15.0% |
| !!! | AAATAATATCGATTTTACAT+TGG | + | chr8.3:29983133-29983152 | None:intergenic | 15.0% |
| !!! | TAGAAAAATGTATACTTTTT+TGG | + | chr8.3:29983095-29983114 | None:intergenic | 15.0% |
| !! | TTATGTTAAGATCCATATAA+TGG | - | chr8.3:29982700-29982719 | MS.gene066463:intron | 20.0% |
| !!! | AAAAATGTATACTTTTTTGG+AGG | + | chr8.3:29983092-29983111 | None:intergenic | 20.0% |
| !!! | TGAATTTTTATCTCTAACAT+GGG | + | chr8.3:29983216-29983235 | None:intergenic | 20.0% |
| !!! | TTGAATTTTTATCTCTAACA+TGG | + | chr8.3:29983217-29983236 | None:intergenic | 20.0% |
| ! | TATATATCACTTTGTGCTTA+TGG | + | chr8.3:29983261-29983280 | None:intergenic | 25.0% |
| !!! | ATTATATGGAAGTGTACTTT+TGG | + | chr8.3:29983521-29983540 | None:intergenic | 25.0% |
| !!! | TAATATCGATTTTACATTGG+AGG | + | chr8.3:29983130-29983149 | None:intergenic | 25.0% |
| !!! | TTATATGGAAGTGTACTTTT+GGG | + | chr8.3:29983520-29983539 | None:intergenic | 25.0% |
| AAAGTTACTTGTGTTATGAG+AGG | + | chr8.3:29983475-29983494 | None:intergenic | 30.0% | |
| AATCAAAGCAATGTCTAGAA+TGG | - | chr8.3:29983631-29983650 | MS.gene066463:CDS | 30.0% | |
| ACAAACACAGATGAAAACAA+AGG | - | chr8.3:29982508-29982527 | MS.gene066463:CDS | 30.0% | |
| ACGTGTTTGCAAAAAACATA+AGG | - | chr8.3:29983044-29983063 | MS.gene066463:intron | 30.0% | |
| ATTGGATGCAAATGAGAATA+TGG | - | chr8.3:29983417-29983436 | MS.gene066463:CDS | 30.0% | |
| CAAACACAGATGAAAACAAA+GGG | - | chr8.3:29982509-29982528 | MS.gene066463:CDS | 30.0% | |
| TGCAAATGAGAATATGGATA+TGG | - | chr8.3:29983423-29983442 | MS.gene066463:CDS | 30.0% | |
| TGTTTGCAAAAAACATAAGG+TGG | - | chr8.3:29983047-29983066 | MS.gene066463:intron | 30.0% | |
| TTGTTCCAAACATTTGTGAA+TGG | + | chr8.3:29982807-29982826 | None:intergenic | 30.0% | |
| ! | ATCAAAGCAATGTCTAGAAT+GGG | - | chr8.3:29983632-29983651 | MS.gene066463:CDS | 30.0% |
| !! | TATATGGAAGTGTACTTTTG+GGG | + | chr8.3:29983519-29983538 | None:intergenic | 30.0% |
| AAACACGTGAAACAAGAGTT+TGG | + | chr8.3:29983032-29983051 | None:intergenic | 35.0% | |
| AACAAGAACAACAATGCAAG+AGG | - | chr8.3:29982822-29982841 | MS.gene066463:CDS | 35.0% | |
| AACACGTGAAACAAGAGTTT+GGG | + | chr8.3:29983031-29983050 | None:intergenic | 35.0% | |
| ACAATAGGAAATGGCTAAAG+TGG | - | chr8.3:29983657-29983676 | MS.gene066463:CDS | 35.0% | |
| ACTTTGTGCTTATGGAACTT+AGG | + | chr8.3:29983253-29983272 | None:intergenic | 35.0% | |
| AGAAAAAGGATCAGGATCAT+TGG | - | chr8.3:29983399-29983418 | MS.gene066463:CDS | 35.0% | |
| AGTCATCTTTGTGATCTTGT+AGG | - | chr8.3:29983553-29983572 | MS.gene066463:intron | 35.0% | |
| ATAGCTGGAAAGCTAGAAAA+AGG | - | chr8.3:29983385-29983404 | MS.gene066463:CDS | 35.0% | |
| ATGGCTAAAGTGGTTGAAAT+TGG | - | chr8.3:29983667-29983686 | MS.gene066463:CDS | 35.0% | |
| ATTAAACTCACCATCTTGCA+GGG | - | chr8.3:29983601-29983620 | MS.gene066463:intron | 35.0% | |
| CTTTCCAGCTATTATAGATG+TGG | + | chr8.3:29983377-29983396 | None:intergenic | 35.0% | |
| GAAACAGTAGCTGCATTATT+AGG | + | chr8.3:29982739-29982758 | None:intergenic | 35.0% | |
| GAGGTGTAAAACAAGAGTTT+GGG | + | chr8.3:29983073-29983092 | None:intergenic | 35.0% | |
| GCTAGAAAATCGAGTAATTC+AGG | - | chr8.3:29982904-29982923 | MS.gene066463:intron | 35.0% | |
| GTGAACCTCTTCAAAAACAT+AGG | + | chr8.3:29982852-29982871 | None:intergenic | 35.0% | |
| TCTTCCACATCTATAATAGC+TGG | - | chr8.3:29983370-29983389 | MS.gene066463:CDS | 35.0% | |
| TGCTTATGGAACTTAGGATA+TGG | + | chr8.3:29983247-29983266 | None:intergenic | 35.0% | |
| TGGCTAAAGTGGTTGAAATT+GGG | - | chr8.3:29983668-29983687 | MS.gene066463:CDS | 35.0% | |
| TGTTGCCATTCACAAATGTT+TGG | - | chr8.3:29982799-29982818 | MS.gene066463:CDS | 35.0% | |
| !! | GAATGGGTTACAATAGGAAA+TGG | - | chr8.3:29983648-29983667 | MS.gene066463:CDS | 35.0% |
| !! | GTTGTCCTATGTTTTTGAAG+AGG | - | chr8.3:29982844-29982863 | MS.gene066463:CDS | 35.0% |
| !! | TGTCTAGAATGGGTTACAAT+AGG | - | chr8.3:29983642-29983661 | MS.gene066463:CDS | 35.0% |
| AATCGTTGGAGAATCGACAA+GGG | + | chr8.3:29982547-29982566 | None:intergenic | 40.0% | |
| ATCGTGTCTGCCAAAACATA+TGG | - | chr8.3:29983002-29983021 | MS.gene066463:intron | 40.0% | |
| CAGACACGATGATACATAGA+CGG | + | chr8.3:29982992-29983011 | None:intergenic | 40.0% | |
| CATTAAACTCACCATCTTGC+AGG | - | chr8.3:29983600-29983619 | MS.gene066463:intron | 40.0% | |
| CTTGTAGGATAGCATTGTTG+TGG | - | chr8.3:29983568-29983587 | MS.gene066463:intron | 40.0% | |
| GGAAAGCTAGAAAAAGGATC+AGG | - | chr8.3:29983391-29983410 | MS.gene066463:CDS | 40.0% | |
| GTAACATCTTCGTAGCTTGA+TGG | - | chr8.3:29983179-29983198 | MS.gene066463:intron | 40.0% | |
| TTTGAAGAGGTTCACCACAA+AGG | - | chr8.3:29982857-29982876 | MS.gene066463:intron | 40.0% | |
| ! | GGAGGTGTAAAACAAGAGTT+TGG | + | chr8.3:29983074-29983093 | None:intergenic | 40.0% |
| !! | CTGAGTATTGACTTGGACAT+GGG | - | chr8.3:29983328-29983347 | MS.gene066463:CDS | 40.0% |
| !! | TCTTCAGCTGAGTATTGACT+TGG | - | chr8.3:29983321-29983340 | MS.gene066463:CDS | 40.0% |
| ATTCTATGTGCCCTGCAAGA+TGG | + | chr8.3:29983614-29983633 | None:intergenic | 45.0% | |
| ATTGGATCGTCTTGAGCTTC+TGG | - | chr8.3:29982564-29982583 | MS.gene066463:CDS | 45.0% | |
| CAATCGTTGGAGAATCGACA+AGG | + | chr8.3:29982548-29982567 | None:intergenic | 45.0% | |
| GTAGGATAGCATTGTTGTGG+AGG | - | chr8.3:29983571-29983590 | MS.gene066463:intron | 45.0% | |
| TGGACATGGGAAACACATCA+AGG | - | chr8.3:29983341-29983360 | MS.gene066463:CDS | 45.0% | |
| ! | GTTTGGGTCGCCATATGTTT+TGG | + | chr8.3:29983015-29983034 | None:intergenic | 45.0% |
| !! | CTTGTCGATTCTCCAACGAT+TGG | - | chr8.3:29982546-29982565 | MS.gene066463:CDS | 45.0% |
| !! | GCTGAGTATTGACTTGGACA+TGG | - | chr8.3:29983327-29983346 | MS.gene066463:CDS | 45.0% |
| !!! | TTTTATTTTATTTTTTTGAA+AGG | - | chr8.3:29982664-29982683 | MS.gene066463:intron | 5.0% |
| GCTCAAGACGATCCAATCGT+TGG | + | chr8.3:29982561-29982580 | None:intergenic | 50.0% | |
| GATCCAGCAACGTGCCTTTG+TGG | + | chr8.3:29982874-29982893 | None:intergenic | 55.0% | |
| ! | TCACCACAAAGGCACGTTGC+TGG | - | chr8.3:29982868-29982887 | MS.gene066463:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 29982505 | 29983697 | 29982505 | ID=MS.gene066463 |
| chr8.3 | mRNA | 29982505 | 29983697 | 29982505 | ID=MS.gene066463.t1;Parent=MS.gene066463 |
| chr8.3 | exon | 29983617 | 29983697 | 29983617 | ID=MS.gene066463.t1.exon1;Parent=MS.gene066463.t1 |
| chr8.3 | CDS | 29983617 | 29983697 | 29983617 | ID=cds.MS.gene066463.t1;Parent=MS.gene066463.t1 |
| chr8.3 | exon | 29983277 | 29983453 | 29983277 | ID=MS.gene066463.t1.exon2;Parent=MS.gene066463.t1 |
| chr8.3 | CDS | 29983277 | 29983453 | 29983277 | ID=cds.MS.gene066463.t1;Parent=MS.gene066463.t1 |
| chr8.3 | exon | 29982716 | 29982874 | 29982716 | ID=MS.gene066463.t1.exon3;Parent=MS.gene066463.t1 |
| chr8.3 | CDS | 29982716 | 29982874 | 29982716 | ID=cds.MS.gene066463.t1;Parent=MS.gene066463.t1 |
| chr8.3 | exon | 29982505 | 29982627 | 29982505 | ID=MS.gene066463.t1.exon4;Parent=MS.gene066463.t1 |
| chr8.3 | CDS | 29982505 | 29982627 | 29982505 | ID=cds.MS.gene066463.t1;Parent=MS.gene066463.t1 |
| Gene Sequence |
| Protein sequence |
