AlfalfaGEDB | MS.gene066463

M. sativa cultivar XinJiangDaYe / MS.gene066463

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066463.t1	XP_003629040.1	75.4	179	44	0	1	179	1	179	1.20E-64	256.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066463.t1	G7L7W4	75.4	179	44	0	1	179	1	179	8.4e-65	256.1

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050807	MS.gene066463	0.811893	5.57E-51	-1.69E-46
MS.gene050826	MS.gene066463	0.805272	1.45E-49	-1.69E-46
MS.gene053563	MS.gene066463	0.804712	1.89E-49	-1.69E-46
MS.gene053581	MS.gene066463	0.800747	1.25E-48	-1.69E-46
MS.gene059439	MS.gene066463	0.811636	6.34E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066463.t1	MTR_8g072450	93.296	179	12	0	1	179	1	179	3.62e-99	284

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 36 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTACTTGTGTTATGAG+AGG	0.587359	8.3:+29982708	None:intergenic
AACAAGAACAACAATGCAAG+AGG	0.640851	8.3:-29983358	MS.gene066463:CDS
AATCAAAGCAATGTCTAGAA+TGG	0.406480	8.3:-29982549	MS.gene066463:CDS
AATCGTTGGAGAATCGACAA+GGG	0.726740	8.3:+29983636	None:intergenic
ACAAACACAGATGAAAACAA+AGG	0.596759	8.3:-29983672	MS.gene066463:CDS
ACAATAGGAAATGGCTAAAG+TGG	0.506453	8.3:-29982523	MS.gene066463:CDS
ATAGCTGGAAAGCTAGAAAA+AGG	0.330571	8.3:-29982795	MS.gene066463:CDS
ATCAAAGCAATGTCTAGAAT+GGG	0.486283	8.3:-29982548	MS.gene066463:CDS
ATGGCTAAAGTGGTTGAAAT+TGG	0.250565	8.3:-29982513	MS.gene066463:CDS
ATTAAACTCACCATCTTGCA+GGG	0.587021	8.3:-29982579	MS.gene066463:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGTTACTTGTGTTATGAG+AGG	+	chr8.3:29983475-29983494	None:intergenic	30.0%
AATCAAAGCAATGTCTAGAA+TGG	-	chr8.3:29983631-29983650	MS.gene066463:CDS	30.0%
ACAAACACAGATGAAAACAA+AGG	-	chr8.3:29982508-29982527	MS.gene066463:CDS	30.0%
ACGTGTTTGCAAAAAACATA+AGG	-	chr8.3:29983044-29983063	MS.gene066463:intron	30.0%
ATTGGATGCAAATGAGAATA+TGG	-	chr8.3:29983417-29983436	MS.gene066463:CDS	30.0%
CAAACACAGATGAAAACAAA+GGG	-	chr8.3:29982509-29982528	MS.gene066463:CDS	30.0%
TGCAAATGAGAATATGGATA+TGG	-	chr8.3:29983423-29983442	MS.gene066463:CDS	30.0%
TGTTTGCAAAAAACATAAGG+TGG	-	chr8.3:29983047-29983066	MS.gene066463:intron	30.0%
TTGTTCCAAACATTTGTGAA+TGG	+	chr8.3:29982807-29982826	None:intergenic	30.0%
AAACACGTGAAACAAGAGTT+TGG	+	chr8.3:29983032-29983051	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	29982505	29983697	29982505	ID=MS.gene066463
chr8.3	mRNA	29982505	29983697	29982505	ID=MS.gene066463.t1;Parent=MS.gene066463
chr8.3	exon	29983617	29983697	29983617	ID=MS.gene066463.t1.exon1;Parent=MS.gene066463.t1
chr8.3	CDS	29983617	29983697	29983617	ID=cds.MS.gene066463.t1;Parent=MS.gene066463.t1
chr8.3	exon	29983277	29983453	29983277	ID=MS.gene066463.t1.exon2;Parent=MS.gene066463.t1
chr8.3	CDS	29983277	29983453	29983277	ID=cds.MS.gene066463.t1;Parent=MS.gene066463.t1
chr8.3	exon	29982716	29982874	29982716	ID=MS.gene066463.t1.exon3;Parent=MS.gene066463.t1
chr8.3	CDS	29982716	29982874	29982716	ID=cds.MS.gene066463.t1;Parent=MS.gene066463.t1
chr8.3	exon	29982505	29982627	29982505	ID=MS.gene066463.t1.exon4;Parent=MS.gene066463.t1
chr8.3	CDS	29982505	29982627	29982505	ID=cds.MS.gene066463.t1;Parent=MS.gene066463.t1

Gene Sequence

>MS.gene066463

ATGACAAACACAGATGAAAACAAAGGGCTTAATAATCAACCCTTGTCGATTCTCCAACGATTGGATCGTCTTGAGCTTCTGGTAAATTAAAATCAAATATTTCTTCTCTAGAGTTTTACATTAATGTATTTTTATTTTATTTTTTGATAGAAAAATGTATTTTATTTTATTTTTTTGAAAGGAGAAAAATGTATTTTATGTTAAGATCCATATAATGGTCATTAATTAAATCCTAATAATGCAGCTACTGTTTCTTGAAAAAAAGCAATGTCACACAATAGAAGATTCTTCTGATGTTGCCATTCACAAATGTTTGGAACAAGAACAACAATGCAAGAGGTTGTCCTATGTTTTTGAAGAGGTTCACCACAAAGGCACGTTGCTGGATCGAGTAGCATTGCTAGAAAATCGAGTAATTCAGGTTTTTGAAGATCCACATAATTTTATTTTTTGTTTAGTGTCATATGTTTTAAAATTTGATATGCCGTCTATGTATCATCGTGTCTGCCAAAACATATGGCGACCCAAACTCTTGTTTCACGTGTTTGCAAAAAACATAAGGTGGCCCAAACTCTTGTTTTACACCTCCAAAAAAGTATACATTTTTCTAAAGGTCTTTAAGCCTCCAATGTAAAATCGATATTATTTAATACTAATAAGTTGATACTTTTTGCGTAACATCTTCGTAGCTTGATGGTCGAAGCAAAACCCATGTTAGAGATAAAAATTCAAATTTCAACCATATCCTAAGTTCCATAAGCACAAAGTGATATATACACATCTATATTATTTATGTATGCTTAATTAGTTTTTGATTCTTCAGCTGAGTATTGACTTGGACATGGGAAACACATCAAGGTCAAGTTCTTCCACATCTATAATAGCTGGAAAGCTAGAAAAAGGATCAGGATCATTGGATGCAAATGAGAATATGGATATGGTCACTAAACTTCAAGAGAAGCATGATCCTCTCATAACACAAGTAACTTTTTGTTAAACTATTGTATATGTCCCCAAAAGTACACTTCCATATAATTTTTTTACTAATAGTCATCTTTGTGATCTTGTAGGATAGCATTGTTGTGGAGGCTTGTTCATTAAACTCACCATCTTGCAGGGCACATAGAATCAAAGCAATGTCTAGAATGGGTTACAATAGGAAATGGCTAAAGTGGTTGAAATTGGGATGTTGA

Protein sequence

>MS.gene066463.t1

MTNTDENKGLNNQPLSILQRLDRLELLLLFLEKKQCHTIEDSSDVAIHKCLEQEQQCKRLSYVFEEVHHKGTLLDRVALLENRVIQLSIDLDMGNTSRSSSSTSIIAGKLEKGSGSLDANENMDMVTKLQEKHDPLITQDSIVVEACSLNSPSCRAHRIKAMSRMGYNRKWLKWLKLGC