Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066878.t1 | RHN40642.1 | 98.4 | 434 | 7 | 0 | 1 | 434 | 1 | 434 | 6.40E-242 | 846.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066878.t1 | B4RRY8 | 34.0 | 406 | 254 | 6 | 30 | 434 | 3 | 395 | 4.4e-58 | 226.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066878.t1 | A0A396GHL0 | 98.4 | 434 | 7 | 0 | 1 | 434 | 1 | 434 | 4.6e-242 | 846.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene066878 | 0.805158 | 1.53E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene066878 | 0.812727 | 3.66E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066878.t1 | AT1G54310 | 72.055 | 433 | 113 | 1 | 9 | 433 | 21 | 453 | 0.0 | 671 |
| MS.gene066878.t1 | AT1G54310 | 70.404 | 223 | 58 | 1 | 9 | 223 | 4 | 226 | 1.40e-112 | 331 |
Find 95 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACATACCTTTGTTCTTTC+AGG | 0.131889 | 8.4:-54373146 | None:intergenic |
| CTCTTTCAGATTATGGAAAA+TGG | 0.172930 | 8.4:+54374029 | MS.gene066878:intron |
| GGGGAAGACGCAACTCTTTA+AGG | 0.181754 | 8.4:+54372225 | MS.gene066878:CDS |
| TTGATGACCTGTTCCTGTTC+TGG | 0.234494 | 8.4:+54377204 | MS.gene066878:CDS |
| ACTATATCAATCATATAAAT+TGG | 0.237392 | 8.4:+54373048 | MS.gene066878:CDS |
| CATATTCATGCGTATCTTAC+AGG | 0.288345 | 8.4:+54377245 | MS.gene066878:CDS |
| GGGTTGATAGTTGATGTATT+TGG | 0.291687 | 8.4:+54372945 | MS.gene066878:CDS |
| AGTTGCATCTTCTTTCAAAA+AGG | 0.293841 | 8.4:-54375497 | None:intergenic |
| CCGCTGTTGAATTGCGTAGA+AGG | 0.300401 | 8.4:+54372569 | MS.gene066878:CDS |
| TAAACTGGCACCTTCGAAAA+AGG | 0.306564 | 8.4:+54375582 | MS.gene066878:CDS |
| CTTGATCTTTGTTGCTATAG+TGG | 0.307349 | 8.4:+54374155 | MS.gene066878:CDS |
| TTGGTTAGGTATGCGCCTTC+CGG | 0.315334 | 8.4:-54378344 | None:intergenic |
| TCCTTTAAAATATCAATAGA+TGG | 0.342814 | 8.4:-54373074 | None:intergenic |
| CTGAAACTCTTGATATAAAC+TGG | 0.352092 | 8.4:-54374118 | None:intergenic |
| AGAACAATATCGCCTGTCTT+AGG | 0.354908 | 8.4:-54372304 | None:intergenic |
| TGAACAAACGGAGAGGGATT+TGG | 0.364849 | 8.4:-54369674 | None:intergenic |
| TGGTTAGGTATGCGCCTTCC+GGG | 0.374670 | 8.4:-54378343 | None:intergenic |
| CTCTTATGAGAGATGAATCT+TGG | 0.380181 | 8.4:+54375536 | MS.gene066878:CDS |
| CGGTTTCTATGTTCTCTGTT+CGG | 0.387086 | 8.4:+54372374 | MS.gene066878:CDS |
| GCAATTTGTTGAAGGGTAGA+TGG | 0.392362 | 8.4:-54369700 | None:intergenic |
| CATCATTGATCCTCCTAAAC+TGG | 0.398344 | 8.4:+54375567 | MS.gene066878:CDS |
| CAGGCTCTACACGGTGCATC+TGG | 0.422808 | 8.4:+54377126 | MS.gene066878:intron |
| ATCACTGTTCTAAGAGATGC+TGG | 0.440246 | 8.4:+54378287 | MS.gene066878:CDS |
| TTGTACAAGCCCCAGCCTAT+TGG | 0.442809 | 8.4:-54372349 | None:intergenic |
| TAGTAGCGTCATCTGCTGCT+TGG | 0.443427 | 8.4:+54372979 | MS.gene066878:CDS |
| GATGAGAAGCAATTTGTTGA+AGG | 0.444343 | 8.4:-54369708 | None:intergenic |
| GTTGAATTGCGTAGAAGGTT+GGG | 0.444805 | 8.4:+54372574 | MS.gene066878:CDS |
| ACGCATGAATATGCCACTTT+GGG | 0.445671 | 8.4:-54377235 | None:intergenic |
| TGTTGAATTGCGTAGAAGGT+TGG | 0.459706 | 8.4:+54372573 | MS.gene066878:CDS |
| GGAGCTGTCGATAGAATTAT+CGG | 0.461674 | 8.4:+54372271 | MS.gene066878:CDS |
| TACGCATGAATATGCCACTT+TGG | 0.462765 | 8.4:-54377236 | None:intergenic |
| GTACAGAAATTTGAATTCGT+TGG | 0.477263 | 8.4:+54377152 | MS.gene066878:CDS |
| GACTGAGAAACCAATAGGCT+GGG | 0.478911 | 8.4:+54372339 | MS.gene066878:CDS |
| CTCTTAACAGAATGTTGGTT+AGG | 0.479278 | 8.4:-54378358 | None:intergenic |
| TCCGGGTATGATGGATCAAT+AGG | 0.479350 | 8.4:-54378326 | None:intergenic |
| TTGGGTCACAGCTCCAGAAC+AGG | 0.480265 | 8.4:-54377217 | None:intergenic |
| CACAGCTCCAGAACAGGAAC+AGG | 0.482052 | 8.4:-54377211 | None:intergenic |
| AGTAGCGTCATCTGCTGCTT+GGG | 0.483757 | 8.4:+54372980 | MS.gene066878:CDS |
| GGACTGAGAAACCAATAGGC+TGG | 0.486780 | 8.4:+54372338 | MS.gene066878:CDS |
| AAGAGTTTCAGATGGTCAAA+AGG | 0.488695 | 8.4:+54374130 | MS.gene066878:CDS |
| TCTTATGAGAGATGAATCTT+GGG | 0.497235 | 8.4:+54375537 | MS.gene066878:CDS |
| TAATCAATAGTGAGGGTGAC+AGG | 0.498128 | 8.4:+54372629 | MS.gene066878:CDS |
| TGAAACTCTTAACAGAATGT+TGG | 0.506506 | 8.4:-54378363 | None:intergenic |
| GGGTTTGGTAGGTGTTGCAA+AGG | 0.511341 | 8.4:+54372189 | MS.gene066878:intron |
| AGATGCAACTCAATTTATGA+AGG | 0.511957 | 8.4:+54375510 | MS.gene066878:CDS |
| TCACTGGCAGGACAGAAGAC+AGG | 0.512939 | 8.4:+54374068 | MS.gene066878:CDS |
| GTACTTAACAACATTGATCC+AGG | 0.515249 | 8.4:+54375466 | MS.gene066878:CDS |
| GATGGGACTGAGAAACCAAT+AGG | 0.515526 | 8.4:+54372334 | MS.gene066878:CDS |
| TATGCGCCTTCCGGGTATGA+TGG | 0.518026 | 8.4:-54378335 | None:intergenic |
| TTCGAAGGTGCCAGTTTAGG+AGG | 0.519285 | 8.4:-54375577 | None:intergenic |
| ACAGCTCCACTGTATACCAT+TGG | 0.521242 | 8.4:-54372256 | None:intergenic |
| AGTTAATGTGTCTGATTCAA+AGG | 0.524828 | 8.4:+54373103 | MS.gene066878:CDS |
| CAGCTCCACTGTATACCATT+GGG | 0.525162 | 8.4:-54372255 | None:intergenic |
| TGCAAAGGTTGTGCTAAAGA+AGG | 0.528824 | 8.4:+54372204 | MS.gene066878:CDS |
| CAGAGATAGAGACATGCATA+AGG | 0.530424 | 8.4:+54373018 | MS.gene066878:CDS |
| AATTGTGTACACAATTTCAC+TGG | 0.536833 | 8.4:+54374052 | MS.gene066878:CDS |
| GGCGGTGCTCTAAATGTCAC+TGG | 0.537655 | 8.4:+54374203 | MS.gene066878:CDS |
| GATATTGTTCTAGTATCAGA+TGG | 0.544300 | 8.4:+54372316 | MS.gene066878:CDS |
| GTGTACACAATTTCACTGGC+AGG | 0.546304 | 8.4:+54374056 | MS.gene066878:CDS |
| GCAAAGGTTGTGCTAAAGAA+GGG | 0.548470 | 8.4:+54372205 | MS.gene066878:CDS |
| GGTAGGCCGCCTCCTAAGAC+AGG | 0.557659 | 8.4:+54372292 | MS.gene066878:CDS |
| GATCTTTGTTGCTATAGTGG+TGG | 0.558098 | 8.4:+54374158 | MS.gene066878:CDS |
| ATGAGAAGCAATTTGTTGAA+GGG | 0.559389 | 8.4:-54369707 | None:intergenic |
| AGCTTGACGACGAAGCTACC+AGG | 0.559455 | 8.4:+54372404 | MS.gene066878:CDS |
| TCTTTAGCAAGCTCAAGAGC+TGG | 0.568312 | 8.4:-54375436 | None:intergenic |
| ATATCAATCATATAAATTGG+AGG | 0.571811 | 8.4:+54373051 | MS.gene066878:CDS |
| TTAGACGATACGCATTCGTA+TGG | 0.574225 | 8.4:-54372609 | None:intergenic |
| GGTAGATGGCAATGAACAAA+CGG | 0.576025 | 8.4:-54369686 | None:intergenic |
| TGGCAATGAACAAACGGAGA+GGG | 0.579038 | 8.4:-54369680 | None:intergenic |
| TTCAGCATGTGCATTGAACA+TGG | 0.582218 | 8.4:+54372519 | MS.gene066878:CDS |
| TACGCATTCGTATGGACAGA+AGG | 0.584507 | 8.4:-54372601 | None:intergenic |
| TCCTATTGATCCATCATACC+CGG | 0.585246 | 8.4:+54378325 | MS.gene066878:CDS |
| TTTATATCAAGAGTTTCAGA+TGG | 0.586095 | 8.4:+54374122 | MS.gene066878:CDS |
| ATATCGCCTGTCTTAGGAGG+CGG | 0.587671 | 8.4:-54372298 | None:intergenic |
| ACAATATCGCCTGTCTTAGG+AGG | 0.593173 | 8.4:-54372301 | None:intergenic |
| CACTGGCAGGACAGAAGACA+GGG | 0.599330 | 8.4:+54374069 | MS.gene066878:CDS |
| AGGCTGGGGCTTGTACAACT+CGG | 0.599441 | 8.4:+54372354 | MS.gene066878:CDS |
| CTTTAAGGATGGCAACCCAA+TGG | 0.617852 | 8.4:+54372240 | MS.gene066878:CDS |
| AAGACGCAACTCTTTAAGGA+TGG | 0.618837 | 8.4:+54372229 | MS.gene066878:CDS |
| ACTGAGAAACCAATAGGCTG+GGG | 0.620801 | 8.4:+54372340 | MS.gene066878:CDS |
| TCTGGAGCTGTGACCCAAAG+TGG | 0.628403 | 8.4:+54377222 | MS.gene066878:CDS |
| GATGCAACTCAATTTATGAA+GGG | 0.634394 | 8.4:+54375511 | MS.gene066878:CDS |
| TACTTGTCCTGAAAGAACAA+AGG | 0.638279 | 8.4:+54373139 | MS.gene066878:CDS |
| CAAAGGTTGTGCTAAAGAAG+GGG | 0.644925 | 8.4:+54372206 | MS.gene066878:CDS |
| GCTCTAAATGCAGTGCGTGG+CGG | 0.645141 | 8.4:+54374185 | MS.gene066878:CDS |
| GTATCGTCTAATCAATAGTG+AGG | 0.646042 | 8.4:+54372621 | MS.gene066878:CDS |
| ATATTGTTCTAGTATCAGAT+GGG | 0.654354 | 8.4:+54372317 | MS.gene066878:CDS |
| ATTGCTTCTCATCAACCCAA+AGG | 0.664853 | 8.4:+54369718 | MS.gene066878:CDS |
| GGCAACCCAATGGTATACAG+TGG | 0.667523 | 8.4:+54372250 | MS.gene066878:CDS |
| ATTGATCCATCATACCCGGA+AGG | 0.668472 | 8.4:+54378329 | MS.gene066878:CDS |
| TATCGTCTAATCAATAGTGA+GGG | 0.677759 | 8.4:+54372622 | MS.gene066878:CDS |
| CTGTCGATAGAATTATCGGT+AGG | 0.678010 | 8.4:+54372275 | MS.gene066878:CDS |
| TTTGCTCTAAATGCAGTGCG+TGG | 0.702286 | 8.4:+54374182 | MS.gene066878:CDS |
| ATGGCAATGAACAAACGGAG+AGG | 0.727516 | 8.4:-54369681 | None:intergenic |
| CCTTCTACGCAATTCAACAG+CGG | 0.768044 | 8.4:-54372569 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 54369658 | 54378388 | 54369658 | ID=MS.gene066878 |
| chr8.4 | mRNA | 54369658 | 54378388 | 54369658 | ID=MS.gene066878.t1;Parent=MS.gene066878 |
| chr8.4 | exon | 54369658 | 54369739 | 54369658 | ID=MS.gene066878.t1.exon1;Parent=MS.gene066878.t1 |
| chr8.4 | CDS | 54369658 | 54369739 | 54369658 | ID=cds.MS.gene066878.t1;Parent=MS.gene066878.t1 |
| chr8.4 | exon | 54372200 | 54372425 | 54372200 | ID=MS.gene066878.t1.exon2;Parent=MS.gene066878.t1 |
| chr8.4 | CDS | 54372200 | 54372425 | 54372200 | ID=cds.MS.gene066878.t1;Parent=MS.gene066878.t1 |
| chr8.4 | exon | 54372516 | 54372650 | 54372516 | ID=MS.gene066878.t1.exon3;Parent=MS.gene066878.t1 |
| chr8.4 | CDS | 54372516 | 54372650 | 54372516 | ID=cds.MS.gene066878.t1;Parent=MS.gene066878.t1 |
| chr8.4 | exon | 54372938 | 54373160 | 54372938 | ID=MS.gene066878.t1.exon4;Parent=MS.gene066878.t1 |
| chr8.4 | CDS | 54372938 | 54373160 | 54372938 | ID=cds.MS.gene066878.t1;Parent=MS.gene066878.t1 |
| chr8.4 | exon | 54374038 | 54374224 | 54374038 | ID=MS.gene066878.t1.exon5;Parent=MS.gene066878.t1 |
| chr8.4 | CDS | 54374038 | 54374224 | 54374038 | ID=cds.MS.gene066878.t1;Parent=MS.gene066878.t1 |
| chr8.4 | exon | 54375419 | 54375603 | 54375419 | ID=MS.gene066878.t1.exon6;Parent=MS.gene066878.t1 |
| chr8.4 | CDS | 54375419 | 54375603 | 54375419 | ID=cds.MS.gene066878.t1;Parent=MS.gene066878.t1 |
| chr8.4 | exon | 54377129 | 54377266 | 54377129 | ID=MS.gene066878.t1.exon7;Parent=MS.gene066878.t1 |
| chr8.4 | CDS | 54377129 | 54377266 | 54377129 | ID=cds.MS.gene066878.t1;Parent=MS.gene066878.t1 |
| chr8.4 | exon | 54378260 | 54378388 | 54378260 | ID=MS.gene066878.t1.exon8;Parent=MS.gene066878.t1 |
| chr8.4 | CDS | 54378260 | 54378388 | 54378260 | ID=cds.MS.gene066878.t1;Parent=MS.gene066878.t1 |
| Gene Sequence |
| Protein sequence |
