AlfalfaGEDB | MS.gene050303

M. sativa cultivar XinJiangDaYe / MS.gene050303

KEGG: K11099
GO: GO:0000387 GO:0005575 GO:0005681 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0022618 GO:0032991 GO:0034622 GO:0043933 GO:0065003 GO:0071826 GO:0071840 GO:1990904
PFAM: PF01423.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050303.t1	XP_003625846.1	100	80	0	0	1	80	1	80	2.50E-35	157.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050303.t1	O82221	91.1	79	7	0	1	79	1	79	1.4e-34	146.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050303.t1	Q2HTF4	100.0	80	0	0	1	80	1	80	1.8e-35	157.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049825	MS.gene050303	0.825854	3.73E-54	-1.69E-46
MS.gene049873	MS.gene050303	0.815657	8.26E-52	-1.69E-46
MS.gene050277	MS.gene050303	0.855956	4.45E-62	-1.69E-46
MS.gene050303	MS.gene050623	-0.802088	6.62E-49	-1.69E-46
MS.gene050303	MS.gene050642	0.81	1.43E-50	-1.69E-46
MS.gene050303	MS.gene050719	0.816197	6.26E-52	-1.69E-46
MS.gene050303	MS.gene050721	0.817517	3.17E-52	-1.69E-46
MS.gene050303	MS.gene050724	0.810876	9.27E-51	-1.69E-46
MS.gene050303	MS.gene050815	0.814078	1.85E-51	-1.69E-46
MS.gene050303	MS.gene051088	0.800255	1.57E-48	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene009798	MS.gene050303	PPI
MS.gene013607	MS.gene050303	PPI
MS.gene015624	MS.gene050303	PPI
MS.gene028393	MS.gene050303	PPI
MS.gene029082	MS.gene050303	PPI
MS.gene030285	MS.gene050303	PPI
MS.gene030697	MS.gene050303	PPI
MS.gene032681	MS.gene050303	PPI
MS.gene046119	MS.gene050303	PPI
MS.gene04711	MS.gene050303	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050303.t1	MTR_7g104930	100.000	80	0	0	1	80	1	80	1.30e-53	161
MS.gene050303.t1	MTR_6g066250	90.476	63	6	0	14	76	15	77	5.20e-35	114
MS.gene050303.t1	MTR_7g065100	38.028	71	35	1	9	70	10	80	9.19e-13	58.5
MS.gene050303.t1	MTR_8g036205	34.722	72	38	1	9	71	10	81	5.11e-11	54.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050303.t1	AT2G23930	91.139	79	7	0	1	79	1	79	2.77e-49	150
MS.gene050303.t1	AT3G11500	91.139	79	7	0	1	79	1	79	4.56e-49	149
MS.gene050303.t1	AT3G11500	91.139	79	7	0	1	79	1	79	4.56e-49	149
MS.gene050303.t1	AT2G23930	88.679	53	6	0	27	79	1	53	1.63e-28	97.1
MS.gene050303.t1	AT2G03870	38.028	71	35	1	9	70	10	80	1.59e-12	57.8
MS.gene050303.t1	AT2G03870	38.028	71	35	1	9	70	10	80	1.59e-12	57.8

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Find 18 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAGTGTGGTCACTGTAG+AGG	0.581425	7.2:-8113273	MS.gene050303:CDS
AATGCAAATCGCATGATTGT+TGG	0.502115	7.2:-8113510	MS.gene050303:CDS
AATGCTTACTTCTTCAAATC+TGG	0.328903	7.2:+8114482	None:intergenic
AATTAGATGCTTCTGTTCAC+TGG	0.418853	7.2:+8113242	None:intergenic
ATGATTGTTGGTACTCTCCG+TGG	0.628986	7.2:-8113498	MS.gene050303:CDS
CAACAACCAAATTCATAAAT+TGG	0.293346	7.2:+8113469	None:intergenic
CGCTTTCTGCAGGTGATCAG+AGG	0.541314	7.2:-8113296	MS.gene050303:intron
CTTCGACCAATTTATGAATT+TGG	0.200064	7.2:-8113475	MS.gene050303:CDS
CTTTCACCATGAGTAGGTCA+GGG	0.590203	7.2:-8114511	None:intergenic
GACAATACTGTGGAGGTGAA+TGG	0.565835	7.2:-8113447	MS.gene050303:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGCTTACTTCTTCAAATC+TGG	+	chr7.2:8113268-8113287	None:intergenic	30.0%
AGGAATTATACTGAAGTATG+TGG	-	chr7.2:8114193-8114212	MS.gene050303:intron	30.0%
CATGTACATAGATACATATC+AGG	+	chr7.2:8114105-8114124	None:intergenic	30.0%
CTTCGACCAATTTATGAATT+TGG	-	chr7.2:8114272-8114291	MS.gene050303:intron	30.0%
GATATGTATCTATGTACATG+TGG	-	chr7.2:8114105-8114124	MS.gene050303:intron	30.0%
GATCCAATTAATAGAAGAAG+TGG	-	chr7.2:8113745-8113764	MS.gene050303:intron	30.0%
GCAATGAGAAAAATGACATA+GGG	-	chr7.2:8114322-8114341	MS.gene050303:intron	30.0%
GGGTTTAACATGTGTATAAA+TGG	-	chr7.2:8114126-8114145	MS.gene050303:intron	30.0%
TGTAATTTCTCTCGCTTATA+TGG	-	chr7.2:8113876-8113895	MS.gene050303:intron	30.0%
TTCATTCCTTTATGAATTGG+GGG	-	chr7.2:8113984-8114003	MS.gene050303:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	8113244	8114525	8113244	ID=MS.gene050303
chr7.2	mRNA	8113244	8114525	8113244	ID=MS.gene050303.t1;Parent=MS.gene050303
chr7.2	exon	8114491	8114525	8114491	ID=MS.gene050303.t1.exon1;Parent=MS.gene050303.t1
chr7.2	CDS	8114491	8114525	8114491	ID=cds.MS.gene050303.t1;Parent=MS.gene050303.t1
chr7.2	exon	8114360	8114382	8114360	ID=MS.gene050303.t1.exon2;Parent=MS.gene050303.t1
chr7.2	CDS	8114360	8114382	8114360	ID=cds.MS.gene050303.t1;Parent=MS.gene050303.t1
chr7.2	exon	8113419	8113540	8113419	ID=MS.gene050303.t1.exon3;Parent=MS.gene050303.t1
chr7.2	CDS	8113419	8113540	8113419	ID=cds.MS.gene050303.t1;Parent=MS.gene050303.t1
chr7.2	exon	8113244	8113306	8113244	ID=MS.gene050303.t1.exon4;Parent=MS.gene050303.t1
chr7.2	CDS	8113244	8113306	8113244	ID=cds.MS.gene050303.t1;Parent=MS.gene050303.t1

Gene Sequence

>MS.gene050303

ATGAGTAGGTCAGGGCAGCCACCAGATTTGAAGAAGTAAGCATTCTTCACACTTTTCAATTCATTTTTTCATTACAATTTTTCGTTGATTTTGAATTTCTACTGCACTTGGTCGATACTGATATGCATTTTTTTTTTCGATAGGTACATGGACAAACAGCTTCAAAGTAAGGTTCTTTCTGCTAATTGTTCTTACTTCTTATCTTCTTTCTTTCTTTGTTTTTTTTTTTTAAATCAAATTTGGTTTTTTGTAGTATTTGTTCAGTTCCTTTTTATATTTATATATATAGTTTGCCATTAAAAAAAAAAAAAAGGTTTTTTGTAGTTTGTGTGAAGATGGAGTGTTTAGTAGAATTGCAAAAATATAATTTTTTAACTGAATTTTTGGTTTTCACTATTGCTATGAGTTCAGTGGTTTCTAGGTACAAGATTGAGTGGGATTTTGATTTTTTATCAATGAATAGCTGAAGATTGTTATGTTCAACTTATAATATTTAAGCATGATCCAATTAATAGAAGAAGTGGTTAAGAATGTATGAGTGTCTGTATGTGAGAGCTTTTACTGGCTGCGAACATTCTTTGGATTGCAGAGAAAAAGTGTGGCATGTCTTTGCTACTGGTTGATACTTTGAATGTAATTTCTCTCGCTTATATGGTAGCTTATATTATTTGGCATTATAAAGTGAAGTTCGAGGAAGTCAATTACTCAATTACAGCTTAATCATTATTTTTTATCCGTTTTTCATTCCTTTATGAATTGGGGGATTACGCTTATTAGTGATTGTGTGAACTATTCATATAAATCTTTCTACGGGTTTGTTTAGTAGTTTTGAATTTTTAAACTGCCTGTCTGTTTGTTCCTGATATGTATCTATGTACATGTGGGTTTAACATGTGTATAAATGGATATTGGTGCTTCTTTGCTGAAAACATGTCTTATGATTTAGCTGAGGAATTATACTGAAGTATGTGGTATTATCTTGCAGTCAAGCTGAATGCAAATCGCATGATTGTTGGTACTCTCCGTGGCTTCGACCAATTTATGAATTTGGTTGTTGACAATACTGTGGAGGTGAATGGCAATGAGAAAAATGACATAGGGATGGTGGTAAGTCCTCATACTATGCATTATCAAGTTATAATATTAGTAGTTTATGTATAGCATCTTATAGAGCTTGAGCTAGGTTTTATATTTGTTAATTTTCACTCGCTTTCTGCAGGTGATCAGAGGAAATAGTGTGGTCACTGTAGAGGCGCTTGAACCAGTGAACAGAAGCATCTAA

Protein sequence

>MS.gene050303.t1

MSRSGQPPDLKKYMDKQLQIKLNANRMIVGTLRGFDQFMNLVVDNTVEVNGNEKNDIGMVVIRGNSVVTVEALEPVNRSI