AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067300.t1 MTR_8g027140 97.416 1006 26 0 1 1006 1 1006 0.0 2002
MS.gene067300.t1 MTR_7g117890 70.348 1005 294 2 2 1006 1 1001 0.0 1423
MS.gene067300.t1 MTR_1g076400 28.332 1013 676 20 11 996 8 997 7.02e-105 351
MS.gene067300.t1 MTR_3g019360 34.639 664 418 6 345 1006 178 827 2.64e-104 345
MS.gene067300.t1 MTR_3g115670 28.086 794 517 24 257 1006 27 810 1.56e-71 254
MS.gene067300.t1 MTR_7g078330 29.492 295 178 7 263 555 278 544 1.93e-19 94.4
MS.gene067300.t1 MTR_7g059405 25.895 363 220 9 261 601 266 601 4.90e-19 92.8
MS.gene067300.t1 MTR_4g063800 29.086 361 211 12 263 590 259 607 3.83e-18 90.1
MS.gene067300.t1 MTR_4g485520 24.523 367 225 9 261 612 107 436 2.56e-16 83.6
MS.gene067300.t1 MTR_4g485520 24.523 367 225 9 261 612 275 604 1.17e-15 82.0
MS.gene067300.t1 MTR_7g053260 26.471 340 213 12 263 595 82 391 1.20e-15 81.3
MS.gene067300.t1 MTR_8g077205 23.229 353 217 9 23 329 1 345 4.60e-15 80.5
MS.gene067300.t1 MTR_2g018010 22.930 471 304 10 89 510 112 572 1.40e-14 79.0
MS.gene067300.t1 MTR_3g466220 25.959 339 200 13 19 338 6 312 9.71e-14 76.3
MS.gene067300.t1 MTR_5g077510 24.569 232 143 6 263 467 39 265 2.53e-13 73.9
MS.gene067300.t1 MTR_3g466220 31.351 185 113 5 156 338 119 291 3.33e-13 74.3
MS.gene067300.t1 MTR_3g095730 24.274 379 204 13 263 602 17 351 1.44e-12 71.2
MS.gene067300.t1 MTR_4g028960 22.676 441 272 16 88 478 112 533 2.17e-12 71.6
MS.gene067300.t1 MTR_4g091880 34.783 92 49 2 261 351 7 88 3.10e-12 69.7
MS.gene067300.t1 MTR_3g008280 32.335 167 89 5 174 328 645 799 3.14e-12 71.2
MS.gene067300.t1 MTR_3g008280 32.335 167 89 5 174 328 653 807 3.24e-12 71.2
MS.gene067300.t1 MTR_4g091880 34.783 92 49 2 261 351 7 88 3.48e-12 69.7
MS.gene067300.t1 MTR_2g011140 24.022 358 243 9 260 596 16 365 6.59e-12 68.9
MS.gene067300.t1 MTR_3g078160 27.111 225 147 5 263 487 279 486 6.94e-12 70.1
MS.gene067300.t1 MTR_3g078160 27.111 225 147 5 263 487 279 486 7.20e-12 70.1
MS.gene067300.t1 MTR_8g092870 43.077 65 37 0 263 327 14 78 1.20e-11 68.6
MS.gene067300.t1 MTR_3g078160 27.111 225 147 5 263 487 279 486 1.68e-11 68.6
MS.gene067300.t1 MTR_1g094215 27.946 297 175 8 263 550 68 334 2.02e-11 67.8
MS.gene067300.t1 MTR_3g096370 34.579 107 61 2 263 360 14 120 2.23e-11 67.4
MS.gene067300.t1 MTR_8g077205 24.561 228 137 5 133 325 2 229 3.08e-11 67.8
MS.gene067300.t1 MTR_4g107010 24.096 332 215 9 239 541 261 584 3.24e-11 67.8
MS.gene067300.t1 MTR_3g065080 39.726 73 44 0 262 334 39 111 3.88e-11 67.0
MS.gene067300.t1 MTR_8g103227 28.467 137 94 3 194 329 333 466 3.91e-11 68.2
MS.gene067300.t1 MTR_5g034440 24.111 253 169 9 93 331 109 352 4.45e-11 67.4
MS.gene067300.t1 MTR_1g017770 32.857 140 84 3 263 402 49 178 4.62e-11 66.6
MS.gene067300.t1 MTR_7g005940 46.269 67 35 1 261 326 597 663 4.97e-11 67.4
MS.gene067300.t1 MTR_7g005940 46.269 67 35 1 261 326 597 663 5.17e-11 67.4
MS.gene067300.t1 MTR_4g085720 39.474 76 39 1 257 325 5 80 6.17e-11 66.2
MS.gene067300.t1 MTR_1g094215 27.645 293 181 7 263 550 251 517 6.47e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067300.t1 AT1G76390 28.535 785 518 25 261 1006 27 807 9.39e-62 226
MS.gene067300.t1 AT1G76390 28.535 785 518 25 261 1006 27 807 9.39e-62 226
MS.gene067300.t1 AT1G20780 27.821 780 522 22 261 1006 25 797 2.79e-57 213
MS.gene067300.t1 AT1G20780 27.821 780 522 22 261 1006 25 797 2.79e-57 213
MS.gene067300.t1 AT1G20780 27.821 780 522 22 261 1006 25 797 2.79e-57 213
MS.gene067300.t1 AT1G68940 24.719 890 597 27 170 1006 216 1085 2.54e-52 200
MS.gene067300.t1 AT1G68940 25.556 810 543 25 170 935 216 1009 8.49e-51 195
MS.gene067300.t1 AT1G68940 25.464 809 545 24 170 935 163 956 1.05e-50 195
MS.gene067300.t1 AT5G42340 25.720 521 305 18 16 492 54 536 1.50e-20 97.8
MS.gene067300.t1 AT1G71020 26.099 364 225 11 261 612 97 428 5.18e-18 89.0
MS.gene067300.t1 AT1G29340 23.983 467 302 10 89 509 124 583 5.63e-18 89.7
MS.gene067300.t1 AT5G67340 22.735 585 373 15 50 591 48 596 6.64e-18 89.4
MS.gene067300.t1 AT5G67340 22.735 585 373 15 50 591 48 596 6.64e-18 89.4
MS.gene067300.t1 AT1G71020 26.099 364 225 11 261 612 245 576 1.22e-17 88.6
MS.gene067300.t1 AT1G60190 23.425 508 338 15 13 479 27 524 3.41e-17 87.0
MS.gene067300.t1 AT3G46510 22.394 518 338 13 13 486 6 503 3.83e-17 87.0
MS.gene067300.t1 AT1G24330 22.712 612 345 17 20 512 3 605 7.69e-17 86.3
MS.gene067300.t1 AT1G24330 22.712 612 345 17 20 512 3 605 7.69e-17 86.3
MS.gene067300.t1 AT1G23030 26.361 349 231 7 263 590 245 588 9.87e-17 85.5
MS.gene067300.t1 AT2G28830 30.233 258 151 7 256 486 181 436 1.55e-15 81.6
MS.gene067300.t1 AT5G01830 23.195 457 294 17 88 505 109 547 6.71e-15 79.7
MS.gene067300.t1 AT1G67530 24.419 344 212 10 25 329 8 342 3.31e-14 77.4
MS.gene067300.t1 AT1G67530 24.198 343 214 9 25 329 8 342 7.22e-14 76.6
MS.gene067300.t1 AT1G67530 24.198 343 214 9 25 329 8 342 7.22e-14 76.6
MS.gene067300.t1 AT1G67530 24.198 343 214 9 25 329 8 342 7.22e-14 76.6
MS.gene067300.t1 AT3G54790 25.279 269 160 10 91 348 86 324 6.38e-13 73.6
MS.gene067300.t1 AT3G54790 25.279 269 160 10 91 348 86 324 6.38e-13 73.6
MS.gene067300.t1 AT1G49780 42.105 76 37 1 257 325 5 80 7.55e-13 72.4
MS.gene067300.t1 AT3G54790 25.279 269 160 10 91 348 50 288 1.05e-12 72.8
MS.gene067300.t1 AT3G07360 23.971 413 231 14 187 587 4 345 1.75e-12 71.2
MS.gene067300.t1 AT4G36550 45.070 71 39 0 261 331 221 291 2.09e-12 71.6
MS.gene067300.t1 AT1G10560 24.784 347 220 11 14 332 28 361 1.52e-11 68.9
MS.gene067300.t1 AT5G51270 36.937 111 52 3 223 329 708 804 1.93e-11 68.9
MS.gene067300.t1 AT5G51270 36.937 111 52 3 223 329 708 804 1.93e-11 68.9
MS.gene067300.t1 AT3G61390 42.647 68 39 0 258 325 277 344 3.65e-11 66.6
MS.gene067300.t1 AT2G23140 29.143 175 111 4 156 329 141 303 3.85e-11 67.8
MS.gene067300.t1 AT2G23140 27.604 192 138 1 401 591 527 718 7.05e-11 67.0
MS.gene067300.t1 AT2G23140 29.143 175 111 4 156 329 138 300 4.01e-11 67.8
MS.gene067300.t1 AT2G23140 27.604 192 138 1 401 591 524 715 7.16e-11 67.0
MS.gene067300.t1 AT2G23140 29.143 175 111 4 156 329 138 300 4.01e-11 67.8
MS.gene067300.t1 AT2G23140 27.604 192 138 1 401 591 524 715 7.16e-11 67.0
MS.gene067300.t1 AT3G61390 42.647 68 39 0 258 325 352 419 5.39e-11 66.6
MS.gene067300.t1 AT2G23140 25.751 233 163 3 401 627 335 563 8.25e-11 66.6
MS.gene067300.t1 AT2G23140 25.751 233 163 3 401 627 335 563 8.25e-11 66.6
MS.gene067300.t1 AT5G09800 41.538 65 38 0 263 327 15 79 9.91e-11 65.5

Find 158 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCGCGCCAAATCATATT+TGG 0.186259 8.2:+70801093 None:intergenic
TGAGTTATCTGATCAATTTC+TGG 0.187668 8.2:+70799317 None:intergenic
TAAGGAACTTAGTTCTTATT+TGG 0.189177 8.2:-70802386 MS.gene067300:CDS
TATGGAGAAAGCTGGATTTA+AGG 0.218387 8.2:-70802077 MS.gene067300:CDS
GCATACACACATCAAGTTTA+TGG 0.223246 8.2:-70800341 MS.gene067300:CDS
TTCTCAGCTCCAAGTGAAAT+TGG 0.232487 8.2:-70798987 MS.gene067300:CDS
GGTCTTTCAGCCGGAATATT+AGG 0.242472 8.2:-70802124 MS.gene067300:CDS
CAAGTTCTTATACAAATTCT+TGG 0.261077 8.2:-70801374 MS.gene067300:CDS
TGGAGTCTGTGAGGCTTCTT+TGG 0.269871 8.2:-70798862 MS.gene067300:CDS
TTTAATTTCTCCTAATATTC+CGG 0.271592 8.2:+70802114 None:intergenic
TCTATCTTTCCATTCTTCTA+TGG 0.273578 8.2:+70801544 None:intergenic
GTTCTTACATAATGATCTTC+AGG 0.295740 8.2:-70800478 MS.gene067300:CDS
ACTTTATAGCACTTGCTCTT+AGG 0.295870 8.2:+70799486 None:intergenic
AAGTCCATTTCTCTTTAAAC+TGG 0.307135 8.2:+70800419 None:intergenic
TGAATTATCCAAATATGATT+TGG 0.309894 8.2:-70801101 MS.gene067300:CDS
GGTTGTGTCACACTGATTAA+AGG 0.311802 8.2:+70800210 None:intergenic
TTCCTCAATTCTTTCAAAAT+CGG 0.318543 8.2:+70802352 None:intergenic
TCAAGGTTGCATACTTCTAT+TGG 0.318559 8.2:-70801056 MS.gene067300:CDS
AATATTAGGAGAAATTAAAA+AGG 0.324360 8.2:-70802110 MS.gene067300:CDS
TTTGCGAAACGGCAATCTTT+AGG 0.329672 8.2:-70801770 MS.gene067300:CDS
GGATCAAATCATTGCTTTGT+TGG 0.331319 8.2:-70801840 MS.gene067300:CDS
TGCTCGTGTTGATAAGAAAA+AGG 0.337238 8.2:-70801879 MS.gene067300:CDS
TTTCTTAGTTCTACATCTCT+TGG 0.339374 8.2:-70798750 MS.gene067300:CDS
TCACACAACCTAACCAATTC+TGG 0.340227 8.2:+70799524 None:intergenic
TTAGTGCAGTTGCTTGAAAT+TGG 0.341628 8.2:-70799116 MS.gene067300:CDS
AATCTTGGAGAATCTGATTA+TGG 0.345438 8.2:-70798882 MS.gene067300:CDS
GGCCAATACATTAGCCGAAA+TGG 0.346085 8.2:-70800565 MS.gene067300:CDS
ATCTTTAGGTACTCGAATCT+TGG 0.353618 8.2:-70801756 MS.gene067300:CDS
TGCAGCAAGTTGCATAATTA+TGG 0.355094 8.2:+70800305 None:intergenic
AAGTACACCACCATCAAATA+AGG 0.363823 8.2:+70800518 None:intergenic
TTTATTTGTTAATGAATTGT+AGG 0.364173 8.2:-70802222 MS.gene067300:CDS
TTCCATTTCGGCTAATGTAT+TGG 0.367156 8.2:+70800563 None:intergenic
TCTCAAATCATAGAAACTAT+TGG 0.367335 8.2:-70802457 MS.gene067300:CDS
TGAACATATTTATAAATGAT+TGG 0.370188 8.2:+70799269 None:intergenic
TGACTGACACAAGGCGTAAA+AGG 0.371641 8.2:+70800161 None:intergenic
CTTCTTCCTTTAGCCGCATC+AGG 0.373069 8.2:-70802145 MS.gene067300:CDS
GGAATTAACTGACCACAATA+AGG 0.387274 8.2:-70800544 MS.gene067300:CDS
AGACCGATTCCGAAGCAAAA+AGG 0.388092 8.2:-70799023 MS.gene067300:CDS
GAATCGATCGTCCATTCTCT+TGG 0.399241 8.2:-70801166 MS.gene067300:CDS
CTATCCAGTTTAAAGAGAAA+TGG 0.399393 8.2:-70800423 MS.gene067300:CDS
GAAAATCGAGTCAGGGATAA+GGG 0.403660 8.2:-70802026 MS.gene067300:CDS
CATACACACATCAAGTTTAT+GGG 0.404356 8.2:-70800340 MS.gene067300:CDS
AGATGATGTAAAGATGATTA+TGG 0.405222 8.2:-70800586 MS.gene067300:CDS
GAAATTGGCTGTAAAGTTCA+TGG 0.408788 8.2:-70798972 MS.gene067300:CDS
GTGGTCAGTTAATTCCATTT+CGG 0.414212 8.2:+70800551 None:intergenic
CTTCATCAGATGAGGATTGT+AGG 0.416308 8.2:+70799378 None:intergenic
CAGCTATACCAGAATTGGTT+AGG 0.419391 8.2:-70799532 MS.gene067300:CDS
AATAAGGAGTCCTTATTTGA+TGG 0.424896 8.2:-70800528 MS.gene067300:CDS
TGATCTGATGAAGTTTCAAC+AGG 0.425758 8.2:+70801683 None:intergenic
AGCTGCAAACCTACTTGCTC+TGG 0.425914 8.2:-70802266 MS.gene067300:CDS
CTAGTAACAAAACCGGTGTT+TGG 0.427966 8.2:+70800262 None:intergenic
TCTGATCAATTTCTGGAAGA+TGG 0.428541 8.2:+70799324 None:intergenic
GTGCTCAGCTATACCAGAAT+TGG 0.431004 8.2:-70799537 MS.gene067300:CDS
TAGATTCTTAAGAGCCTTTG+TGG 0.431900 8.2:+70800443 None:intergenic
AGAAAATCGAGTCAGGGATA+AGG 0.432371 8.2:-70802027 MS.gene067300:CDS
CCATTCTCTTGGACGGCGTC+AGG 0.433963 8.2:-70801155 MS.gene067300:CDS
TTCTTAGTTCTACATCTCTT+GGG 0.436279 8.2:-70798749 MS.gene067300:CDS
TATTTCAGTTTCCTTTCGTT+CGG 0.443580 8.2:+70801794 None:intergenic
GGACCACTCACAAGAAATCT+TGG 0.446995 8.2:-70798897 MS.gene067300:CDS
TCCTATCACACATGATGTTA+TGG 0.452740 8.2:-70801711 MS.gene067300:CDS
TTCGCGATTCATAATATCAA+TGG 0.454754 8.2:+70802294 None:intergenic
AAAGGCTGAAGCTATGCAAA+TGG 0.456067 8.2:-70801861 MS.gene067300:CDS
AATCTCATTGCTAACGCTGT+TGG 0.459825 8.2:-70801965 MS.gene067300:CDS
AGTCTCTACATGCTTTAGAA+AGG 0.467873 8.2:-70798716 MS.gene067300:CDS
ATCAACATTCTCTACTATGA+TGG 0.468562 8.2:+70799430 None:intergenic
ACCATAACATCATGTGTGAT+AGG 0.470871 8.2:+70801710 None:intergenic
GTTGTGTCACACTGATTAAA+GGG 0.473597 8.2:+70800211 None:intergenic
TGGATTTAAGGCTGCGGTAG+CGG 0.475565 8.2:-70802065 MS.gene067300:CDS
CTCAAAACCGATGTATCTAA+AGG 0.478675 8.2:+70801590 None:intergenic
ATTGTAGGTCGATTGTAAAT+CGG 0.479909 8.2:-70802207 MS.gene067300:CDS
GAACACATTGGAAAAGTTCA+AGG 0.482125 8.2:-70801073 MS.gene067300:CDS
TCACACTGATTAAAGGGAAA+AGG 0.486884 8.2:+70800217 None:intergenic
TAGAGATGCAACTGATGTAT+TGG 0.486984 8.2:-70800999 MS.gene067300:CDS
TGTGATAATATGGAGAAAGC+TGG 0.488266 8.2:-70802085 MS.gene067300:CDS
GCAGAGTTCAAACAGTTGTA+TGG 0.490896 8.2:-70798681 MS.gene067300:CDS
GTGAGCAGAGAGAGCAACAC+AGG 0.492544 8.2:-70801424 MS.gene067300:CDS
AGAGGAAATACATTCTGCTA+TGG 0.492784 8.2:-70799357 MS.gene067300:CDS
CTATTGGTGACTATGTCTAA+TGG 0.493151 8.2:-70801040 MS.gene067300:CDS
AGAGAAGTGCAATAGAGAAG+TGG 0.495497 8.2:-70801652 MS.gene067300:CDS
CCTGACGCCGTCCAAGAGAA+TGG 0.499586 8.2:+70801155 None:intergenic
TTACTGCAACGTCTCTCGAC+AGG 0.500509 8.2:-70800914 MS.gene067300:intron
GTTCGAGAAACCGTGACATA+AGG 0.502733 8.2:-70801352 MS.gene067300:CDS
ATAGAGAAGTGGTTTGAAGA+AGG 0.503302 8.2:-70801641 MS.gene067300:CDS
GCTTTATTAACATTGATTGA+AGG 0.507058 8.2:-70798837 MS.gene067300:CDS
TGCCAATACTCTTGCTGACA+TGG 0.507732 8.2:+70798599 None:intergenic
TGTAAGAACAAGTGAAGAAG+AGG 0.509834 8.2:+70800492 None:intergenic
GCCGCATCAGGTCTTTCAGC+CGG 0.509865 8.2:-70802133 MS.gene067300:CDS
AGAGAGAGCAACACAGGGAA+TGG 0.509905 8.2:-70801418 MS.gene067300:CDS
GAAAGACCTGATGCGGCTAA+AGG 0.513497 8.2:+70802139 None:intergenic
ACTTTACAGCCAATTTCACT+TGG 0.514907 8.2:+70798978 None:intergenic
AACTGAAATACTTTGCGAAA+CGG 0.517151 8.2:-70801781 MS.gene067300:CDS
ATCCATGTCAGCAAGAGTAT+TGG 0.518034 8.2:-70798601 MS.gene067300:CDS
TTTCCTTTCGTTCGGTGATG+AGG 0.520051 8.2:+70801802 None:intergenic
TCAGAATCTAGTAACAAAAC+CGG 0.522197 8.2:+70800255 None:intergenic
AGTTACTCCTTTGAAGATCT+CGG 0.524906 8.2:+70799234 None:intergenic
ACAATCCTCATCTGATGAAG+AGG 0.529667 8.2:-70799375 MS.gene067300:CDS
AAAAGATAATGAAGAAGCTA+AGG 0.530212 8.2:-70801297 MS.gene067300:intron
TGAAACAATCCATAGAAGAA+TGG 0.531982 8.2:-70801553 MS.gene067300:CDS
GAGGAAATACATTCTGCTAT+GGG 0.532753 8.2:-70799356 MS.gene067300:CDS
TCTCCAAGATTTCTTGTGAG+TGG 0.533221 8.2:+70798894 None:intergenic
TTGATGTGTGTATGCTATGA+TGG 0.534750 8.2:+70800349 None:intergenic
TCTAAAGGAATCAGCGTCAA+AGG 0.539843 8.2:+70801605 None:intergenic
AAATGAGAAGTCGACGATGA+AGG 0.546935 8.2:-70801936 MS.gene067300:CDS
GACTTAACTCAGAGGAGTAA+TGG 0.550076 8.2:-70798633 MS.gene067300:CDS
TATTTCCTCTTCATCAGATG+AGG 0.554394 8.2:+70799370 None:intergenic
GAGATCAAGCAACGGTCGCA+CGG 0.556041 8.2:+70800377 None:intergenic
TGAGAAATTGCAGTTGAGGT+AGG 0.556553 8.2:+70802475 None:intergenic
CAGTTCCAACGCACTTGGAG+TGG 0.557899 8.2:-70799175 MS.gene067300:CDS
GAAGGTGAAAGACTAGAGAG+TGG 0.557912 8.2:-70798819 MS.gene067300:CDS
ACTCAATGGATTCTAGATGC+TGG 0.565189 8.2:-70799299 MS.gene067300:CDS
GCTGATTCCTTTAGATACAT+CGG 0.566870 8.2:-70801597 MS.gene067300:CDS
CAGCCTCATCACCGAACGAA+AGG 0.569243 8.2:-70801805 MS.gene067300:CDS
TGATCAGAATGTTATACTGA+TGG 0.569744 8.2:-70800960 MS.gene067300:CDS
TTAATCAGTGTGACACAACC+TGG 0.570231 8.2:-70800207 MS.gene067300:CDS
TGGAACTGTAAAACGACGTA+AGG 0.573612 8.2:+70799190 None:intergenic
GATTTGAGAAATTGCAGTTG+AGG 0.578462 8.2:+70802471 None:intergenic
TTCTCTTGGACGGCGTCAGG+AGG 0.580890 8.2:-70801152 MS.gene067300:CDS
AACTTAGTCGAGAAAGCAGT+CGG 0.581164 8.2:-70799215 MS.gene067300:CDS
TTGGACGGCGTCAGGAGGAG+AGG 0.587550 8.2:-70801147 MS.gene067300:CDS
CGATCGTCCATTCTCTTGGA+CGG 0.587926 8.2:-70801162 MS.gene067300:CDS
AAGGAGATAAGCAAGGCGCT+TGG 0.588368 8.2:-70802169 MS.gene067300:CDS
TGTGTATGCTATGATGGTAA+AGG 0.588633 8.2:+70800355 None:intergenic
AACTAGAGGCATTTGAGCTG+AGG 0.594631 8.2:+70798656 None:intergenic
TCCGGCTGAAAGACCTGATG+CGG 0.596788 8.2:+70802132 None:intergenic
TATCAAGACGAAGCTAAATG+AGG 0.597576 8.2:-70800124 MS.gene067300:intron
GAATGTTATACTGATGGCAA+AGG 0.598582 8.2:-70800954 MS.gene067300:CDS
GGTGAAAGACTAGAGAGTGG+TGG 0.599671 8.2:-70798816 MS.gene067300:CDS
GGACAATAAAATGACTGTAG+AGG 0.605850 8.2:+70801731 None:intergenic
CAAATAAGGACTCCTTATTG+TGG 0.607893 8.2:+70800532 None:intergenic
GAAAGCTGGATTTAAGGCTG+CGG 0.609553 8.2:-70802071 MS.gene067300:CDS
GTAGAGACCGAGATCTTCAA+AGG 0.609913 8.2:-70799241 MS.gene067300:CDS
AAATTGATCAGATAACTCAA+TGG 0.612214 8.2:-70799313 MS.gene067300:CDS
GTACATCATTAGCACTGCAT+AGG 0.612506 8.2:+70802428 None:intergenic
GAGCTCGATTCCTTATGTCA+CGG 0.612669 8.2:+70801342 None:intergenic
AAGGAGTCCTTATTTGATGG+TGG 0.615690 8.2:-70800525 MS.gene067300:CDS
ATTTATAAATATGTTCAAGA+CGG 0.616007 8.2:-70799263 MS.gene067300:CDS
CTCTGAGTTAAGTCAACTAG+AGG 0.627340 8.2:+70798642 None:intergenic
ACTTGAAATGATTCGACAAG+GGG 0.629255 8.2:-70800400 MS.gene067300:CDS
GGTGAAAACAGTGGCCACAA+AGG 0.630926 8.2:-70800457 MS.gene067300:CDS
GACTTGAAATGATTCGACAA+GGG 0.634541 8.2:-70800401 MS.gene067300:CDS
CTCTCAAGATGTCCAAACAC+CGG 0.634839 8.2:-70800274 MS.gene067300:CDS
GGACTTGAAATGATTCGACA+AGG 0.635847 8.2:-70800402 MS.gene067300:CDS
TACGACATTCCAGAGCAAGT+AGG 0.646446 8.2:+70802257 None:intergenic
AGACGAAGGTGACTGACACA+AGG 0.652496 8.2:+70800152 None:intergenic
GATTCATAATATCAATGGCA+CGG 0.654224 8.2:+70802299 None:intergenic
TATCACACATGATGTTATGG+TGG 0.655301 8.2:-70801708 MS.gene067300:CDS
GATTTGGCGCGAGAACACAT+TGG 0.659835 8.2:-70801085 MS.gene067300:CDS
CAGGGAATGGATGATACTTG+AGG 0.659956 8.2:-70801405 MS.gene067300:CDS
TGAGCAGAGAGAGCAACACA+GGG 0.661066 8.2:-70801423 MS.gene067300:CDS
ATCTGATTATGGAGTCTGTG+AGG 0.673837 8.2:-70798871 MS.gene067300:CDS
GTCTTGATATTTGAAGACGA+AGG 0.676219 8.2:+70800138 None:intergenic
TCACACGAAGGAGATAAGCA+AGG 0.684993 8.2:-70802176 MS.gene067300:CDS
CTCTAGTTGACTTAACTCAG+AGG 0.686980 8.2:-70798641 MS.gene067300:CDS
TGATCTTCAGGTGAAAACAG+TGG 0.704631 8.2:-70800466 MS.gene067300:CDS
TCGGTTAGAAAATCACACGA+AGG 0.714647 8.2:-70802188 MS.gene067300:CDS
GGAATGTCGTAAGAAGAGCA+AGG 0.723044 8.2:-70802245 MS.gene067300:CDS
TGGTAAAGGAGATCAAGCAA+CGG 0.735449 8.2:+70800369 None:intergenic
ATTGGCTGTAAAGTTCATGG+AGG 0.759451 8.2:-70798969 MS.gene067300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTAAGAAATTTTATTATTAA+TGG + chr8.2:70802303-70802322 None:intergenic 10.0%
!! AATATTAGGAGAAATTAAAA+AGG - chr8.2:70798955-70798974 MS.gene067300:CDS 15.0%
!! ACTAGTATTAAAATAGAAAA+TGG + chr8.2:70799814-70799833 None:intergenic 15.0%
!! ATTTATAAATATGTTCAAGA+CGG - chr8.2:70801802-70801821 MS.gene067300:CDS 15.0%
!! TGAACATATTTATAAATGAT+TGG + chr8.2:70801799-70801818 None:intergenic 15.0%
!!! AATGACATTTTATAATTTTG+GGG + chr8.2:70801083-70801102 None:intergenic 15.0%
!!! GAATGACATTTTATAATTTT+GGG + chr8.2:70801084-70801103 None:intergenic 15.0%
!!! TGAATGACATTTTATAATTT+TGG + chr8.2:70801085-70801104 None:intergenic 15.0%
!!! TTTATTTGTTAATGAATTGT+AGG - chr8.2:70798843-70798862 MS.gene067300:CDS 15.0%
!! AAAGAAAAAATACATCAGTT+TGG - chr8.2:70799571-70799590 MS.gene067300:intron 20.0%
!! AATTTCAACAACAATTTAGA+TGG - chr8.2:70800315-70800334 MS.gene067300:CDS 20.0%
!! ATGAAAATAGTTTCAACATT+CGG + chr8.2:70801454-70801473 None:intergenic 20.0%
!! ATTTGCAAGTAATTAATATC+TGG + chr8.2:70801275-70801294 None:intergenic 20.0%
!! CTAATTTACCTATGAAATTT+AGG - chr8.2:70801212-70801231 MS.gene067300:intron 20.0%
!! GTTAACTGAATATAAAATTC+AGG + chr8.2:70801312-70801331 None:intergenic 20.0%
!! TAAAAAGGTTTGTGATAATA+TGG - chr8.2:70798970-70798989 MS.gene067300:CDS 20.0%
!! TATAATGACATTAAACAATG+TGG + chr8.2:70800438-70800457 None:intergenic 20.0%
!! TCCAAAACTATTTATAACTA+CGG + chr8.2:70800982-70801001 None:intergenic 20.0%
!! TGAATTATCCAAATATGATT+TGG - chr8.2:70799964-70799983 MS.gene067300:intron 20.0%
!! TGTCATATTTATTAATCATC+AGG + chr8.2:70800376-70800395 None:intergenic 20.0%
!!! TGTGAATAAAGATAGTTTTA+AGG - chr8.2:70798661-70798680 MS.gene067300:CDS 20.0%
!!! TTTAATTTCTCCTAATATTC+CGG + chr8.2:70798954-70798973 None:intergenic 20.0%
! AAAAGATAATGAAGAAGCTA+AGG - chr8.2:70799768-70799787 MS.gene067300:intron 25.0%
! AAATTGATCAGATAACTCAA+TGG - chr8.2:70801752-70801771 MS.gene067300:CDS 25.0%
! AACAATTTAGATGGTTAACA+CGG - chr8.2:70800324-70800343 MS.gene067300:CDS 25.0%
! AGATGATGTAAAGATGATTA+TGG - chr8.2:70800479-70800498 MS.gene067300:CDS 25.0%
! CAAGTTCTTATACAAATTCT+TGG - chr8.2:70799691-70799710 MS.gene067300:intron 25.0%
! GATCGAAAATTTACAATCTT+CGG - chr8.2:70801379-70801398 MS.gene067300:CDS 25.0%
! TATAGAAAAAGAACTAGCAA+TGG + chr8.2:70800229-70800248 None:intergenic 25.0%
! TCGACAATAAAGTTAACAAA+TGG - chr8.2:70801348-70801367 MS.gene067300:CDS 25.0%
! TCTCAAATCATAGAAACTAT+TGG - chr8.2:70798608-70798627 MS.gene067300:CDS 25.0%
! TTCCTCAATTCTTTCAAAAT+CGG + chr8.2:70798716-70798735 None:intergenic 25.0%
!! ACCGTAGTTATAAATAGTTT+TGG - chr8.2:70800978-70800997 MS.gene067300:CDS 25.0%
!! GCTTTATTAACATTGATTGA+AGG - chr8.2:70802228-70802247 MS.gene067300:CDS 25.0%
!! TAAGGAACTTAGTTCTTATT+TGG - chr8.2:70798679-70798698 MS.gene067300:CDS 25.0%
!! TTATACATGATTTTCTATGC+AGG - chr8.2:70800453-70800472 MS.gene067300:CDS 25.0%
!!! GATTGTTTCAAAGTTTTGTT+AGG + chr8.2:70799502-70799521 None:intergenic 25.0%
!!! TAAATTAGTTTCTAGTGAAC+TGG + chr8.2:70801200-70801219 None:intergenic 25.0%
!!! TATAAAGTTGTTTTCATGCT+TGG - chr8.2:70801594-70801613 MS.gene067300:CDS 25.0%
AACTGAAATACTTTGCGAAA+CGG - chr8.2:70799284-70799303 MS.gene067300:CDS 30.0%
AAGTCCATTTCTCTTTAAAC+TGG + chr8.2:70800649-70800668 None:intergenic 30.0%
AAGTTGTCTAAAAACTCTTG+AGG - chr8.2:70799612-70799631 MS.gene067300:intron 30.0%
AATCTTGGAGAATCTGATTA+TGG - chr8.2:70802183-70802202 MS.gene067300:CDS 30.0%
ACAATGTGGTTAATTGTGTT+CGG + chr8.2:70800424-70800443 None:intergenic 30.0%
ACATACATTAGTTGAGTCAT+TGG - chr8.2:70800193-70800212 MS.gene067300:CDS 30.0%
ATAGTTTCAACATTCGGAAA+AGG + chr8.2:70801448-70801467 None:intergenic 30.0%
ATCAACATTCTCTACTATGA+TGG + chr8.2:70801638-70801657 None:intergenic 30.0%
ATCATCGAAAATGCAAACAT+CGG - chr8.2:70801018-70801037 MS.gene067300:CDS 30.0%
ATGCAAACATCGGTATAAAT+AGG - chr8.2:70801028-70801047 MS.gene067300:CDS 30.0%
ATTAGTTGAGTCATTGGTAT+TGG - chr8.2:70800199-70800218 MS.gene067300:CDS 30.0%
ATTGTAGGTCGATTGTAAAT+CGG - chr8.2:70798858-70798877 MS.gene067300:CDS 30.0%
CAAAAAATTGTAGCTGAAAC+TGG - chr8.2:70801913-70801932 MS.gene067300:CDS 30.0%
CATACACACATCAAGTTTAT+GGG - chr8.2:70800725-70800744 MS.gene067300:intron 30.0%
CTATCCAGTTTAAAGAGAAA+TGG - chr8.2:70800642-70800661 MS.gene067300:intron 30.0%
GATTCATAATATCAATGGCA+CGG + chr8.2:70798769-70798788 None:intergenic 30.0%
GTTCTTACATAATGATCTTC+AGG - chr8.2:70800587-70800606 MS.gene067300:CDS 30.0%
TATTTCAGTTTCCTTTCGTT+CGG + chr8.2:70799274-70799293 None:intergenic 30.0%
TCAGAATCTAGTAACAAAAC+CGG + chr8.2:70800813-70800832 None:intergenic 30.0%
TCTATCTTTCCATTCTTCTA+TGG + chr8.2:70799524-70799543 None:intergenic 30.0%
TGAAACAATCCATAGAAGAA+TGG - chr8.2:70799512-70799531 MS.gene067300:CDS 30.0%
TGAGTTATCTGATCAATTTC+TGG + chr8.2:70801751-70801770 None:intergenic 30.0%
TGATCAGAATGTTATACTGA+TGG - chr8.2:70800105-70800124 MS.gene067300:intron 30.0%
TGTCATTCAAAATAGTCCAT+CGG - chr8.2:70801096-70801115 MS.gene067300:CDS 30.0%
TTCGCGATTCATAATATCAA+TGG + chr8.2:70798774-70798793 None:intergenic 30.0%
TTGCCAATTCGAAAAAATGA+GGG + chr8.2:70801054-70801073 None:intergenic 30.0%
TTTGCCAATTCGAAAAAATG+AGG + chr8.2:70801055-70801074 None:intergenic 30.0%
! AACTAAACTTTCTTTTCTGC+AGG - chr8.2:70799847-70799866 MS.gene067300:intron 30.0%
! AATAAGGAGTCCTTATTTGA+TGG - chr8.2:70800537-70800556 MS.gene067300:CDS 30.0%
! CAAGTCATCATTTTGTCTTT+TGG - chr8.2:70802131-70802150 MS.gene067300:CDS 30.0%
! TATCTTTTGCTAGAATGCAA+AGG + chr8.2:70799756-70799775 None:intergenic 30.0%
! TATGAAATTTAGGTTACCTC+CGG - chr8.2:70801222-70801241 MS.gene067300:intron 30.0%
!! AAGAAAGTTTAGTTCTCGAA+TGG + chr8.2:70799841-70799860 None:intergenic 30.0%
!! AGAAAGTTTAGTTCTCGAAT+GGG + chr8.2:70799840-70799859 None:intergenic 30.0%
!! ATAATGTTTTGTTGTACACC+AGG + chr8.2:70800879-70800898 None:intergenic 30.0%
!! ATTTTAGAGAAAATCGAGTC+AGG - chr8.2:70799031-70799050 MS.gene067300:CDS 30.0%
!! TTCTTAGTTCTACATCTCTT+GGG - chr8.2:70802316-70802335 MS.gene067300:CDS 30.0%
!! TTTCTTAGTTCTACATCTCT+TGG - chr8.2:70802315-70802334 MS.gene067300:CDS 30.0%
!! TTTTAGAGAAAATCGAGTCA+GGG - chr8.2:70799032-70799051 MS.gene067300:CDS 30.0%
AAGTACACCACCATCAAATA+AGG + chr8.2:70800550-70800569 None:intergenic 35.0%
ACCATAACATCATGTGTGAT+AGG + chr8.2:70799358-70799377 None:intergenic 35.0%
ACTTGAAATGATTCGACAAG+GGG - chr8.2:70800665-70800684 MS.gene067300:intron 35.0%
ACTTTACAGCCAATTTCACT+TGG + chr8.2:70802090-70802109 None:intergenic 35.0%
ACTTTATAGCACTTGCTCTT+AGG + chr8.2:70801582-70801601 None:intergenic 35.0%
AGAGGAAATACATTCTGCTA+TGG - chr8.2:70801708-70801727 MS.gene067300:CDS 35.0%
ATAGAGAAGTGGTTTGAAGA+AGG - chr8.2:70799424-70799443 MS.gene067300:CDS 35.0%
ATCTTTAGGTACTCGAATCT+TGG - chr8.2:70799309-70799328 MS.gene067300:CDS 35.0%
CAAATAAGGACTCCTTATTG+TGG + chr8.2:70800536-70800555 None:intergenic 35.0%
CTCAAAACCGATGTATCTAA+AGG + chr8.2:70799478-70799497 None:intergenic 35.0%
GAAATTGGCTGTAAAGTTCA+TGG - chr8.2:70802093-70802112 MS.gene067300:CDS 35.0%
GAACACATTGGAAAAGTTCA+AGG - chr8.2:70799992-70800011 MS.gene067300:intron 35.0%
GAATGTTATACTGATGGCAA+AGG - chr8.2:70800111-70800130 MS.gene067300:intron 35.0%
GACTTGAAATGATTCGACAA+GGG - chr8.2:70800664-70800683 MS.gene067300:intron 35.0%
GAGGAAATACATTCTGCTAT+GGG - chr8.2:70801709-70801728 MS.gene067300:CDS 35.0%
GATTTGAGAAATTGCAGTTG+AGG + chr8.2:70798597-70798616 None:intergenic 35.0%
GCATACACACATCAAGTTTA+TGG - chr8.2:70800724-70800743 MS.gene067300:intron 35.0%
GGAATTAACTGACCACAATA+AGG - chr8.2:70800521-70800540 MS.gene067300:CDS 35.0%
GGACAATAAAATGACTGTAG+AGG + chr8.2:70799337-70799356 None:intergenic 35.0%
GGAGGTAACCTAAATTTCAT+AGG + chr8.2:70801223-70801242 None:intergenic 35.0%
GGATCAAATCATTGCTTTGT+TGG - chr8.2:70799225-70799244 MS.gene067300:CDS 35.0%
GTCTTGATATTTGAAGACGA+AGG + chr8.2:70800930-70800949 None:intergenic 35.0%
GTGGTCAGTTAATTCCATTT+CGG + chr8.2:70800517-70800536 None:intergenic 35.0%
GTTAACACGGTTCAAATATG+TGG - chr8.2:70800337-70800356 MS.gene067300:CDS 35.0%
TAGAGATGCAACTGATGTAT+TGG - chr8.2:70800066-70800085 MS.gene067300:intron 35.0%
TAGATTCTTAAGAGCCTTTG+TGG + chr8.2:70800625-70800644 None:intergenic 35.0%
TATCAAGACGAAGCTAAATG+AGG - chr8.2:70800941-70800960 MS.gene067300:CDS 35.0%
TATCACACATGATGTTATGG+TGG - chr8.2:70799357-70799376 MS.gene067300:CDS 35.0%
TATGGAGAAAGCTGGATTTA+AGG - chr8.2:70798988-70799007 MS.gene067300:CDS 35.0%
TATTTCCTCTTCATCAGATG+AGG + chr8.2:70801698-70801717 None:intergenic 35.0%
TCAAGGTTGCATACTTCTAT+TGG - chr8.2:70800009-70800028 MS.gene067300:intron 35.0%
TCCTATCACACATGATGTTA+TGG - chr8.2:70799354-70799373 MS.gene067300:CDS 35.0%
TGAAAAAGTGAGTGATTCTG+AGG - chr8.2:70798736-70798755 MS.gene067300:CDS 35.0%
TGATCTGATGAAGTTTCAAC+AGG + chr8.2:70799385-70799404 None:intergenic 35.0%
TGCAGCAAGTTGCATAATTA+TGG + chr8.2:70800763-70800782 None:intergenic 35.0%
TGTAAGAACAAGTGAAGAAG+AGG + chr8.2:70800576-70800595 None:intergenic 35.0%
TGTGATAATATGGAGAAAGC+TGG - chr8.2:70798980-70798999 MS.gene067300:CDS 35.0%
TGTGTATGCTATGATGGTAA+AGG + chr8.2:70800713-70800732 None:intergenic 35.0%
TTAGTGCAGTTGCTTGAAAT+TGG - chr8.2:70801949-70801968 MS.gene067300:CDS 35.0%
TTCCATTTCGGCTAATGTAT+TGG + chr8.2:70800505-70800524 None:intergenic 35.0%
TTGATGTGTGTATGCTATGA+TGG + chr8.2:70800719-70800738 None:intergenic 35.0%
! AGTTACTCCTTTGAAGATCT+CGG + chr8.2:70801834-70801853 None:intergenic 35.0%
! ATGAAATTTAGGTTACCTCC+GGG - chr8.2:70801223-70801242 MS.gene067300:intron 35.0%
! CATAGTAGTCCTTTTTGCTT+CGG + chr8.2:70802054-70802073 None:intergenic 35.0%
! CTATTGGTGACTATGTCTAA+TGG - chr8.2:70800025-70800044 MS.gene067300:intron 35.0%
! GCTGATTCCTTTAGATACAT+CGG - chr8.2:70799468-70799487 MS.gene067300:CDS 35.0%
! GTTGCAGATTTTCTTAGTTC+TGG - chr8.2:70798566-70798585 MS.gene067300:CDS 35.0%
! GTTGTGTCACACTGATTAAA+GGG + chr8.2:70800857-70800876 None:intergenic 35.0%
! TCACACTGATTAAAGGGAAA+AGG + chr8.2:70800851-70800870 None:intergenic 35.0%
! TCTGATCAATTTCTGGAAGA+TGG + chr8.2:70801744-70801763 None:intergenic 35.0%
! TGCTCGTGTTGATAAGAAAA+AGG - chr8.2:70799186-70799205 MS.gene067300:CDS 35.0%
!! AGTCTCTACATGCTTTAGAA+AGG - chr8.2:70802349-70802368 MS.gene067300:CDS 35.0%
!! CGCTGAATTTTCTAAAAGCT+CGG - chr8.2:70802008-70802027 MS.gene067300:CDS 35.0%
AAAGGCTGAAGCTATGCAAA+TGG - chr8.2:70799204-70799223 MS.gene067300:CDS 40.0%
AAATGAGAAGTCGACGATGA+AGG - chr8.2:70799129-70799148 MS.gene067300:CDS 40.0%
AACTTAGTCGAGAAAGCAGT+CGG - chr8.2:70801850-70801869 MS.gene067300:CDS 40.0%
AATCTCATTGCTAACGCTGT+TGG - chr8.2:70799100-70799119 MS.gene067300:CDS 40.0%
AATTACTGACAAACTTGCCC+CGG + chr8.2:70801244-70801263 None:intergenic 40.0%
ACAATCCTCATCTGATGAAG+AGG - chr8.2:70801690-70801709 MS.gene067300:CDS 40.0%
AGAAAATCGAGTCAGGGATA+AGG - chr8.2:70799038-70799057 MS.gene067300:CDS 40.0%
ATCTGATTATGGAGTCTGTG+AGG - chr8.2:70802194-70802213 MS.gene067300:CDS 40.0%
ATTGGCTGTAAAGTTCATGG+AGG - chr8.2:70802096-70802115 MS.gene067300:CDS 40.0%
CAGCTATACCAGAATTGGTT+AGG - chr8.2:70801533-70801552 MS.gene067300:CDS 40.0%
CTAGTAACAAAACCGGTGTT+TGG + chr8.2:70800806-70800825 None:intergenic 40.0%
CTATGATGTGGCACGTTAAT+TGG - chr8.2:70801139-70801158 MS.gene067300:CDS 40.0%
CTCTGAGTTAAGTCAACTAG+AGG + chr8.2:70802426-70802445 None:intergenic 40.0%
CTTCATCAGATGAGGATTGT+AGG + chr8.2:70801690-70801709 None:intergenic 40.0%
GAAAATCGAGTCAGGGATAA+GGG - chr8.2:70799039-70799058 MS.gene067300:CDS 40.0%
GACTTAACTCAGAGGAGTAA+TGG - chr8.2:70802432-70802451 MS.gene067300:CDS 40.0%
GCAGAGTTCAAACAGTTGTA+TGG - chr8.2:70802384-70802403 MS.gene067300:CDS 40.0%
GGACTTGAAATGATTCGACA+AGG - chr8.2:70800663-70800682 MS.gene067300:intron 40.0%
GTACATCATTAGCACTGCAT+AGG + chr8.2:70798640-70798659 None:intergenic 40.0%
TAACGACAGAAGTTAACCGA+TGG + chr8.2:70801115-70801134 None:intergenic 40.0%
TCACACAACCTAACCAATTC+TGG + chr8.2:70801544-70801563 None:intergenic 40.0%
TCGGTTAGAAAATCACACGA+AGG - chr8.2:70798877-70798896 MS.gene067300:CDS 40.0%
TCTAAAGGAATCAGCGTCAA+AGG + chr8.2:70799463-70799482 None:intergenic 40.0%
TCTCCAAGATTTCTTGTGAG+TGG + chr8.2:70802174-70802193 None:intergenic 40.0%
TGAGAAATTGCAGTTGAGGT+AGG + chr8.2:70798593-70798612 None:intergenic 40.0%
TGATCTTCAGGTGAAAACAG+TGG - chr8.2:70800599-70800618 MS.gene067300:intron 40.0%
TGGAACTGTAAAACGACGTA+AGG + chr8.2:70801878-70801897 None:intergenic 40.0%
TGGTAAAGGAGATCAAGCAA+CGG + chr8.2:70800699-70800718 None:intergenic 40.0%
TTAATCAGTGTGACACAACC+TGG - chr8.2:70800858-70800877 MS.gene067300:intron 40.0%
TTCTCAGCTCCAAGTGAAAT+TGG - chr8.2:70802078-70802097 MS.gene067300:CDS 40.0%
TTCTCGCGCCAAATCATATT+TGG + chr8.2:70799975-70799994 None:intergenic 40.0%
TTTGCGAAACGGCAATCTTT+AGG - chr8.2:70799295-70799314 MS.gene067300:CDS 40.0%
! AAGGAGTCCTTATTTGATGG+TGG - chr8.2:70800540-70800559 MS.gene067300:CDS 40.0%
! ACTCAATGGATTCTAGATGC+TGG - chr8.2:70801766-70801785 MS.gene067300:CDS 40.0%
! AGAGAAGTGCAATAGAGAAG+TGG - chr8.2:70799413-70799432 MS.gene067300:CDS 40.0%
! AGTCCTTTTTGCTTCGGAAT+CGG + chr8.2:70802048-70802067 None:intergenic 40.0%
! ATCCATGTCAGCAAGAGTAT+TGG - chr8.2:70802464-70802483 MS.gene067300:CDS 40.0%
! CTCTAGTTGACTTAACTCAG+AGG - chr8.2:70802424-70802443 MS.gene067300:CDS 40.0%
! GAGCACTGTTCAAACATAAG+TGG + chr8.2:70801514-70801533 None:intergenic 40.0%
! GGTTGTGTCACACTGATTAA+AGG + chr8.2:70800858-70800877 None:intergenic 40.0%
! TGAAATTTAGGTTACCTCCG+GGG - chr8.2:70801224-70801243 MS.gene067300:intron 40.0%
! TTTTACAGTTCCAACGCACT+TGG - chr8.2:70801885-70801904 MS.gene067300:CDS 40.0%
!!! AGGCCCTCATTTTTTCGAAT+TGG - chr8.2:70801048-70801067 MS.gene067300:CDS 40.0%
!!! CGCCGATTTTGAAAGAATTG+AGG - chr8.2:70798711-70798730 MS.gene067300:CDS 40.0%
ATTCGGAAAAGGCAAGTGCA+TGG + chr8.2:70801437-70801456 None:intergenic 45.0%
CAGGGAATGGATGATACTTG+AGG - chr8.2:70799660-70799679 MS.gene067300:intron 45.0%
CGGTTAACTTCTGTCGTTAG+AGG - chr8.2:70801116-70801135 MS.gene067300:CDS 45.0%
CTCTCAAGATGTCCAAACAC+CGG - chr8.2:70800791-70800810 MS.gene067300:intron 45.0%
GAAGGTGAAAGACTAGAGAG+TGG - chr8.2:70802246-70802265 MS.gene067300:CDS 45.0%
GAATCGATCGTCCATTCTCT+TGG - chr8.2:70799899-70799918 MS.gene067300:intron 45.0%
GAGCTCGATTCCTTATGTCA+CGG + chr8.2:70799726-70799745 None:intergenic 45.0%
GGAATGTCGTAAGAAGAGCA+AGG - chr8.2:70798820-70798839 MS.gene067300:CDS 45.0%
GGACCACTCACAAGAAATCT+TGG - chr8.2:70802168-70802187 MS.gene067300:CDS 45.0%
GGCCAATACATTAGCCGAAA+TGG - chr8.2:70800500-70800519 MS.gene067300:CDS 45.0%
GGTCTTTCAGCCGGAATATT+AGG - chr8.2:70798941-70798960 MS.gene067300:CDS 45.0%
GTAGAGACCGAGATCTTCAA+AGG - chr8.2:70801824-70801843 MS.gene067300:CDS 45.0%
GTGCTCAGCTATACCAGAAT+TGG - chr8.2:70801528-70801547 MS.gene067300:CDS 45.0%
GTTCGAGAAACCGTGACATA+AGG - chr8.2:70799713-70799732 MS.gene067300:intron 45.0%
TACGACATTCCAGAGCAAGT+AGG + chr8.2:70798811-70798830 None:intergenic 45.0%
TCACACGAAGGAGATAAGCA+AGG - chr8.2:70798889-70798908 MS.gene067300:CDS 45.0%
TGACTGACACAAGGCGTAAA+AGG + chr8.2:70800907-70800926 None:intergenic 45.0%
TGCCAATACTCTTGCTGACA+TGG + chr8.2:70802469-70802488 None:intergenic 45.0%
TGTCGTTAGAGGCTATGATG+TGG - chr8.2:70801127-70801146 MS.gene067300:CDS 45.0%
! AGACCGATTCCGAAGCAAAA+AGG - chr8.2:70802042-70802061 MS.gene067300:CDS 45.0%
! CTTTTGGCAGCTGAAGCAAT+TGG - chr8.2:70802147-70802166 MS.gene067300:CDS 45.0%
! GAAAGCTGGATTTAAGGCTG+CGG - chr8.2:70798994-70799013 MS.gene067300:CDS 45.0%
! TTTCCTTTCGTTCGGTGATG+AGG + chr8.2:70799266-70799285 None:intergenic 45.0%
!! AACTAGAGGCATTTGAGCTG+AGG + chr8.2:70802412-70802431 None:intergenic 45.0%
AGACGAAGGTGACTGACACA+AGG + chr8.2:70800916-70800935 None:intergenic 50.0%
AGAGAGAGCAACACAGGGAA+TGG - chr8.2:70799647-70799666 MS.gene067300:intron 50.0%
AGCTGCAAACCTACTTGCTC+TGG - chr8.2:70798799-70798818 MS.gene067300:CDS 50.0%
CGATCGTCCATTCTCTTGGA+CGG - chr8.2:70799903-70799922 MS.gene067300:intron 50.0%
CTTCTTCCTTTAGCCGCATC+AGG - chr8.2:70798920-70798939 MS.gene067300:CDS 50.0%
GAAAGACCTGATGCGGCTAA+AGG + chr8.2:70798929-70798948 None:intergenic 50.0%
GATTTGGCGCGAGAACACAT+TGG - chr8.2:70799980-70799999 MS.gene067300:intron 50.0%
GGTGAAAACAGTGGCCACAA+AGG - chr8.2:70800608-70800627 MS.gene067300:intron 50.0%
GGTGAAAGACTAGAGAGTGG+TGG - chr8.2:70802249-70802268 MS.gene067300:CDS 50.0%
TGAGCAGAGAGAGCAACACA+GGG - chr8.2:70799642-70799661 MS.gene067300:intron 50.0%
TGGAGTCTGTGAGGCTTCTT+TGG - chr8.2:70802203-70802222 MS.gene067300:CDS 50.0%
TTACTGCAACGTCTCTCGAC+AGG - chr8.2:70800151-70800170 MS.gene067300:CDS 50.0%
! AAGGAGATAAGCAAGGCGCT+TGG - chr8.2:70798896-70798915 MS.gene067300:CDS 50.0%
! TGGATTTAAGGCTGCGGTAG+CGG - chr8.2:70799000-70799019 MS.gene067300:CDS 50.0%
! TTTTGCCACTCCAAGTGCGT+TGG + chr8.2:70801898-70801917 None:intergenic 50.0%
!! CGCTGCACGTTGTTTTGTCA+AGG + chr8.2:70801980-70801999 None:intergenic 50.0%
CAGCCTCATCACCGAACGAA+AGG - chr8.2:70799260-70799279 MS.gene067300:CDS 55.0%
CAGTTCCAACGCACTTGGAG+TGG - chr8.2:70801890-70801909 MS.gene067300:CDS 55.0%
GAGATCAAGCAACGGTCGCA+CGG + chr8.2:70800691-70800710 None:intergenic 55.0%
GTGAGCAGAGAGAGCAACAC+AGG - chr8.2:70799641-70799660 MS.gene067300:intron 55.0%
TACTGACAAACTTGCCCCGG+AGG + chr8.2:70801241-70801260 None:intergenic 55.0%
TCCGGCTGAAAGACCTGATG+CGG + chr8.2:70798936-70798955 None:intergenic 55.0%
CCATTCTCTTGGACGGCGTC+AGG - chr8.2:70799910-70799929 MS.gene067300:intron 60.0%
CCTGACGCCGTCCAAGAGAA+TGG + chr8.2:70799913-70799932 None:intergenic 60.0%
GCCGCATCAGGTCTTTCAGC+CGG - chr8.2:70798932-70798951 MS.gene067300:CDS 60.0%
TTCTCTTGGACGGCGTCAGG+AGG - chr8.2:70799913-70799932 MS.gene067300:intron 60.0%
TTGGACGGCGTCAGGAGGAG+AGG - chr8.2:70799918-70799937 MS.gene067300:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 70798560 70802527 70798560 ID=MS.gene067300
chr8.2 mRNA 70798560 70802527 70798560 ID=MS.gene067300.t1;Parent=MS.gene067300
chr8.2 exon 70801298 70802527 70801298 ID=MS.gene067300.t1.exon1;Parent=MS.gene067300.t1
chr8.2 CDS 70801298 70802527 70801298 ID=cds.MS.gene067300.t1;Parent=MS.gene067300.t1
chr8.2 exon 70800915 70801218 70800915 ID=MS.gene067300.t1.exon2;Parent=MS.gene067300.t1
chr8.2 CDS 70800915 70801218 70800915 ID=cds.MS.gene067300.t1;Parent=MS.gene067300.t1
chr8.2 exon 70800125 70800612 70800125 ID=MS.gene067300.t1.exon3;Parent=MS.gene067300.t1
chr8.2 CDS 70800125 70800612 70800125 ID=cds.MS.gene067300.t1;Parent=MS.gene067300.t1
chr8.2 exon 70798560 70799558 70798560 ID=MS.gene067300.t1.exon4;Parent=MS.gene067300.t1
chr8.2 CDS 70798560 70799558 70798560 ID=cds.MS.gene067300.t1;Parent=MS.gene067300.t1
Gene Sequence

>MS.gene067300

ATGATGGTTGCAGATTTTCTTAGTTCTGGTCCTACCTCAACTGCAATTTCTCAAATCATAGAAACTATTGGTGAGTTCCTATGCAGTGCTAATGATGTACTTGTGAATAAAGATAGTTTTAAGGAACTTAGTTCTTATTTGGAAAGAATTACGCCGATTTTGAAAGAATTGAGGAATGAAAAAGTGAGTGATTCTGAGGCATTCAACCGTGCCATTGATATTATGAATCGCGAAACGAAAGCTGCAAACCTACTTGCTCTGGAATGTCGTAAGAAGAGCAAGGTTTATTTGTTAATGAATTGTAGGTCGATTGTAAATCGGTTAGAAAATCACACGAAGGAGATAAGCAAGGCGCTTGGTCTTCTTCCTTTAGCCGCATCAGGTCTTTCAGCCGGAATATTAGGAGAAATTAAAAAGGTTTGTGATAATATGGAGAAAGCTGGATTTAAGGCTGCGGTAGCGGAAGAAGAGATTTTAGAGAAAATCGAGTCAGGGATAAGGGAAAACAGTTTTGATCGTAAGCATGCAAATAATTTAATAAATCTCATTGCTAACGCTGTTGGAATTACAAATGAGAAGTCGACGATGAAGGCTGAACTTGAAGAGTTTAAGAAAGAAATCGAAAATGCTCGTGTTGATAAGAAAAAGGCTGAAGCTATGCAAATGGATCAAATCATTGCTTTGTTGGAAAGAGCTGATGCAGCCTCATCACCGAACGAAAGGAAACTGAAATACTTTGCGAAACGGCAATCTTTAGGTACTCGAATCTTGGAGCCTCTACAGTCATTTTATTGTCCTATCACACATGATGTTATGGTGGAACCTGTTGAAACTTCATCAGATCAAACATTTGAGAGAAGTGCAATAGAGAAGTGGTTTGAAGAAGGAAACAAGTTGTGTCCTTTGACGCTGATTCCTTTAGATACATCGGTTTTGAGACCTAACAAAACTTTGAAACAATCCATAGAAGAATGGAAAGATAGAAATACAATGATTACAATTGCTACTTTGAAAGAAAAAATACATCAGTTTGGAGATGATGATGAAGTGATAAGTTGTCTAAAAACTCTTGAGGATTTGTGTGAGCAGAGAGAGCAACACAGGGAATGGATGATACTTGAGGACTACATACAAGTTCTTATACAAATTCTTGGTTCGAGAAACCGTGACATAAGGAATCGAGCTCTTGTCATCCTTTGCATTCTAGCAAAAGATAATGAAGAAGCTAAGGTACTTAAATGAAAATACTATCCATTTTCTATTTTAATACTAGTTTACCCATTCGAGAACTAAACTTTCTTTTCTGCAGGAAAGAATTGTAACAGTTGATAATGCGATTGAATCGATCGTCCATTCTCTTGGACGGCGTCAGGAGGAGAGGAAGTTAGCAGTTGCATTACTGCTTGAATTATCCAAATATGATTTGGCGCGAGAACACATTGGAAAAGTTCAAGGTTGCATACTTCTATTGGTGACTATGTCTAATGGTGATGACAATCAAGCTGCTAGAGATGCAACTGATGTATTGGATAATCTTTCATACTCTGATCAGAATGTTATACTGATGGCAAAGGCAAACTATTTCAAGCATTTACTGCAACGTCTCTCGACAGGTTTGTTTAATCTTTTATCTACATACATTAGTTGAGTCATTGGTATTGGTGTTCCATTGCTAGTTCTTTTTCTATAAAAGATATGTGTGTGTAACATAAAAGAGCAGTGATACAAATATCAGAAATGACACTTTGACGCACAAATTTCAACAACAATTTAGATGGTTAACACGGTTCAAATATGTGGTTAATTACGTTCACCTGATGATTAATAAATATGACAAGTTAATGAAACTCGTTAATCTTTACCGAACACAATTAACCACATTGTTTAATGTCATTATACATGATTTTCTATGCAGGAGCAGATGATGTAAAGATGATTATGGCCAATACATTAGCCGAAATGGAATTAACTGACCACAATAAGGAGTCCTTATTTGATGGTGGTGTACTTGCTCCTCTTCTTCACTTGTTCTTACATAATGATCTTCAGGTGAAAACAGTGGCCACAAAGGCTCTTAAGAATCTATCCAGTTTAAAGAGAAATGGACTTGAAATGATTCGACAAGGGGCCGTGCGACCGTTGCTTGATCTCCTTTACCATCATAGCATACACACATCAAGTTTATGGGAAGATGTAGCAGCCATAATTATGCAACTTGCTGCATCAACTATCTCTCAAGATGTCCAAACACCGGTTTTGTTACTAGATTCTGATGATGATGTTTTTAACCTTTTCCCTTTAATCAGTGTGACACAACCTGGTGTACAACAAAACATTATTCAGACCTTTTACGCCTTGTGTCAGTCACCTTCGTCTTCAAATATCAAGACGAAGCTAAATGAGGTATGAATCTTACTGACCGTAGTTATAAATAGTTTTGGAGTGAATATGCAGATTGATCATCGAAAATGCAAACATCGGTATAAATAGGCCCTCATTTTTTCGAATTGGCAAATACATCCCCAAAATTATAAAATGTCATTCAAAATAGTCCATCGGTTAACTTCTGTCGTTAGAGGCTATGATGTGGCACGTTAATTGGACATGTCAAATTGCACATTAATCTCACATCGATGTCCAGTTCACTAGAAACTAATTTACCTATGAAATTTAGGTTACCTCCGGGGCAAGTTTGTCAGTAATTGTAATTGTCCAGATATTAATTACTTGCAAATTAGTCTTTCAGAGACCTGAATTTTATATTCAGTTAACGTGTCGCGTCGTAGTCTCGACAATAAAGTTAACAAATGGACTATTTTGATCGAAAATTTACAATCTTCGGTTTTTAAAGTTCATTCTCTACTATTCTATCATCCATGCACTTGCCTTTTCCGAATGTTGAAACTATTTTCATGTTTTTGTTTATGTCAATATACTGTAACTAAACGAGTCCACTTATGTTTGAACAGTGCTCAGCTATACCAGAATTGGTTAGGTTGTGTGAGAATGAGAATCTAAACCTAAGAGCAAGTGCTATAAAGTTGTTTTCATGCTTGGTAGAGAGTTGTGATGAATCCATCATAGTAGAGAATGTTGATCAGAAATGCATCAATAATTTACTTCAGATCCTACAATCCTCATCTGATGAAGAGGAAATACATTCTGCTATGGGAATAATATGCCATCTTCCAGAAATTGATCAGATAACTCAATGGATTCTAGATGCTGGCGTACTTCCAATCATTTATAAATATGTTCAAGACGGTAGAGACCGAGATCTTCAAAGGAGTAACTTAGTCGAGAAAGCAGTCGGTGCCTTACGTCGTTTTACAGTTCCAACGCACTTGGAGTGGCAAAAAATTGTAGCTGAAACTGGTATTATAACTGTTTTAGTGCAGTTGCTTGAAATTGGATCAACCTTGACAAAACAACGTGCAGCGTTATGTCTCGCTGAATTTTCTAAAAGCTCGGTTAGTCTCAGCAGACCGATTCCGAAGCAAAAAGGACTACTATGTTGTTTCTCAGCTCCAAGTGAAATTGGCTGTAAAGTTCATGGAGGAGTATGCACTGTCAAGTCATCATTTTGTCTTTTGGCAGCTGAAGCAATTGGACCACTCACAAGAAATCTTGGAGAATCTGATTATGGAGTCTGTGAGGCTTCTTTGGATGCTTTATTAACATTGATTGAAGGTGAAAGACTAGAGAGTGGTGGTAATGTGCTTGCTAAAGCAAATGCAATACCATTAATAATAAAATTTCTTAGTTCTACATCTCTTGGGTTGCAAGAAAAGTCTCTACATGCTTTAGAAAGGATTTTTCAGCTAGCAGAGTTCAAACAGTTGTATGGAGCCTCAGCTCAAATGCCTCTAGTTGACTTAACTCAGAGGAGTAATGGTAGAGTTAGATCCATGTCAGCAAGAGTATTGGCACATTTGAATGTTCTTCATGATCAGTCTTCATACTTTTAA
Protein sequence

>MS.gene067300.t1

MMVADFLSSGPTSTAISQIIETIGEFLCSANDVLVNKDSFKELSSYLERITPILKELRNEKVSDSEAFNRAIDIMNRETKAANLLALECRKKSKVYLLMNCRSIVNRLENHTKEISKALGLLPLAASGLSAGILGEIKKVCDNMEKAGFKAAVAEEEILEKIESGIRENSFDRKHANNLINLIANAVGITNEKSTMKAELEEFKKEIENARVDKKKAEAMQMDQIIALLERADAASSPNERKLKYFAKRQSLGTRILEPLQSFYCPITHDVMVEPVETSSDQTFERSAIEKWFEEGNKLCPLTLIPLDTSVLRPNKTLKQSIEEWKDRNTMITIATLKEKIHQFGDDDEVISCLKTLEDLCEQREQHREWMILEDYIQVLIQILGSRNRDIRNRALVILCILAKDNEEAKERIVTVDNAIESIVHSLGRRQEERKLAVALLLELSKYDLAREHIGKVQGCILLLVTMSNGDDNQAARDATDVLDNLSYSDQNVILMAKANYFKHLLQRLSTGADDVKMIMANTLAEMELTDHNKESLFDGGVLAPLLHLFLHNDLQVKTVATKALKNLSSLKRNGLEMIRQGAVRPLLDLLYHHSIHTSSLWEDVAAIIMQLAASTISQDVQTPVLLLDSDDDVFNLFPLISVTQPGVQQNIIQTFYALCQSPSSSNIKTKLNECSAIPELVRLCENENLNLRASAIKLFSCLVESCDESIIVENVDQKCINNLLQILQSSSDEEEIHSAMGIICHLPEIDQITQWILDAGVLPIIYKYVQDGRDRDLQRSNLVEKAVGALRRFTVPTHLEWQKIVAETGIITVLVQLLEIGSTLTKQRAALCLAEFSKSSVSLSRPIPKQKGLLCCFSAPSEIGCKVHGGVCTVKSSFCLLAAEAIGPLTRNLGESDYGVCEASLDALLTLIEGERLESGGNVLAKANAIPLIIKFLSSTSLGLQEKSLHALERIFQLAEFKQLYGASAQMPLVDLTQRSNGRVRSMSARVLAHLNVLHDQSSYF