AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067303

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067303.t1 MTR_8g027200 90.307 423 15 1 1 423 59 455 0.0 767
MS.gene067303.t1 MTR_7g117950 63.397 418 125 9 1 416 61 452 0.0 526
MS.gene067303.t1 MTR_3g092060 52.785 413 185 6 4 414 66 470 3.18e-156 451
MS.gene067303.t1 MTR_2g061820 36.410 390 212 5 1 356 57 444 1.17e-86 273
MS.gene067303.t1 MTR_4g128260 33.810 420 225 9 1 371 59 474 5.83e-71 233
MS.gene067303.t1 MTR_8g076920 35.912 362 199 10 4 338 81 436 4.75e-68 224
MS.gene067303.t1 MTR_5g020980 34.031 382 217 6 34 388 100 473 3.28e-66 219
MS.gene067303.t1 MTR_1g022250 34.264 394 206 10 7 357 69 452 8.40e-66 218
MS.gene067303.t1 MTR_2g076690 36.257 342 182 10 41 356 104 435 2.75e-65 216
MS.gene067303.t1 MTR_2g030420 34.190 389 208 10 7 357 72 450 5.92e-65 215
MS.gene067303.t1 MTR_6g009720 33.770 382 194 8 7 335 71 446 1.12e-64 216
MS.gene067303.t1 MTR_2g076840 32.199 382 220 10 1 356 58 426 1.68e-64 220
MS.gene067303.t1 MTR_2g076840 41.935 248 129 6 122 357 460 704 5.57e-58 202
MS.gene067303.t1 MTR_6g009520 32.658 395 219 10 7 357 71 462 7.62e-64 214
MS.gene067303.t1 MTR_1g022245 34.188 351 179 7 34 335 91 438 1.06e-63 213
MS.gene067303.t1 MTR_2g076970 36.824 296 170 5 70 356 141 428 2.01e-63 211
MS.gene067303.t1 MTR_7g008040 34.133 375 197 9 29 356 89 460 6.52e-63 212
MS.gene067303.t1 MTR_2g076710 34.604 341 194 8 41 356 102 438 6.71e-63 209
MS.gene067303.t1 MTR_4g107370 32.326 430 216 10 31 403 95 506 6.93e-63 211
MS.gene067303.t1 MTR_6g009770 31.695 407 216 9 7 357 48 448 9.60e-63 210
MS.gene067303.t1 MTR_6g009553 31.785 409 215 9 7 357 47 449 1.08e-62 210
MS.gene067303.t1 MTR_2g076920 34.670 349 193 9 33 356 90 428 2.03e-62 209
MS.gene067303.t1 MTR_6g009540 31.671 401 227 9 1 357 63 460 2.34e-62 210
MS.gene067303.t1 MTR_5g020990 32.902 386 203 10 17 356 95 470 6.52e-62 208
MS.gene067303.t1 MTR_2g076900 30.971 381 233 8 1 356 58 433 1.80e-60 203
MS.gene067303.t1 MTR_2g076990 30.287 383 230 8 1 356 1 373 1.75e-59 200
MS.gene067303.t1 MTR_6g042290 33.239 355 204 8 7 335 81 428 3.59e-58 198
MS.gene067303.t1 MTR_3g103150 39.759 249 133 5 122 356 31 276 6.10e-58 192
MS.gene067303.t1 MTR_6g009510 31.622 370 201 8 1 356 75 406 2.52e-54 187
MS.gene067303.t1 MTR_6g009660 29.223 373 190 11 29 357 70 412 6.94e-44 159
MS.gene067303.t1 MTR_5g021020 31.731 312 178 12 56 356 118 405 3.11e-39 145
MS.gene067303.t1 MTR_5g020720 37.415 147 77 4 191 324 25 169 4.60e-21 90.9
MS.gene067303.t1 MTR_5g021000 31.214 173 103 5 251 421 1 159 5.94e-18 81.3
MS.gene067303.t1 MTR_1g011680 35.417 96 60 2 240 335 18 111 1.26e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067303.t1 AT2G46620 58.548 427 160 6 1 415 60 481 5.74e-167 478
MS.gene067303.t1 AT4G25835 37.280 397 205 8 1 356 59 452 8.91e-76 245
MS.gene067303.t1 AT5G57480 35.349 430 217 10 4 375 62 488 2.36e-74 241
MS.gene067303.t1 AT5G17760 41.391 302 151 8 71 356 163 454 5.52e-69 227
MS.gene067303.t1 AT3G28580 36.182 351 180 7 30 338 91 439 3.51e-68 225
MS.gene067303.t1 AT2G18193 38.690 336 171 9 34 343 101 427 4.12e-67 222
MS.gene067303.t1 AT5G40010 35.054 368 189 9 27 348 91 454 1.55e-66 221
MS.gene067303.t1 AT4G30250 34.250 400 214 8 4 356 62 459 2.24e-66 221
MS.gene067303.t1 AT4G30250 34.250 400 214 8 4 356 64 461 2.34e-66 221
MS.gene067303.t1 AT3G50930 35.933 359 189 10 34 357 148 500 3.61e-66 221
MS.gene067303.t1 AT3G28580 36.280 328 189 6 30 338 91 417 1.92e-65 217
MS.gene067303.t1 AT3G28610 36.905 336 174 10 37 343 106 432 5.35e-65 216
MS.gene067303.t1 AT3G28600 36.888 347 185 11 37 356 107 446 5.93e-65 216
MS.gene067303.t1 AT3G50940 35.758 330 180 7 34 338 102 424 9.23e-65 214
MS.gene067303.t1 AT5G17750 36.364 341 179 7 13 322 57 390 3.21e-64 211
MS.gene067303.t1 AT5G17730 36.086 327 176 9 73 386 155 461 5.71e-64 213
MS.gene067303.t1 AT5G17740 31.529 425 231 12 3 390 71 472 1.02e-63 214
MS.gene067303.t1 AT2G18190 36.474 329 181 7 34 338 102 426 1.26e-63 213
MS.gene067303.t1 AT5G40000 35.652 345 191 11 37 356 111 449 6.70e-61 205
MS.gene067303.t1 AT3G28540 33.721 344 182 8 33 335 96 434 8.46e-61 206
MS.gene067303.t1 AT3G28540 33.721 344 182 8 33 335 96 434 1.04e-60 205
MS.gene067303.t1 AT3G28510 32.970 367 200 8 33 356 95 458 1.19e-60 206
MS.gene067303.t1 AT4G05380 37.500 336 176 8 47 356 120 447 1.85e-60 203
MS.gene067303.t1 AT3G28600 39.568 278 141 7 104 356 132 407 2.55e-59 200
MS.gene067303.t1 AT3G28570 33.807 352 210 9 22 356 88 433 3.68e-59 200
MS.gene067303.t1 AT3G28570 33.807 352 210 9 22 356 95 440 4.71e-59 199
MS.gene067303.t1 AT1G43910 33.951 324 180 6 47 343 119 435 9.45e-54 186
MS.gene067303.t1 AT3G28520 32.945 343 188 8 33 338 85 422 3.84e-52 182
MS.gene067303.t1 AT5G17760 48.428 159 75 2 71 229 163 314 2.73e-40 147
MS.gene067303.t1 AT3G29800 28.267 329 207 10 27 342 55 367 8.07e-37 140
MS.gene067303.t1 AT4G05340 47.826 69 35 1 242 310 27 94 1.91e-13 66.2

Find 101 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATGGTTTGTTTAGATTA+AGG 0.215457 8.2:-70823065 MS.gene067303:CDS
AAGGATTTGAGGAAGATTTA+TGG 0.229987 8.2:-70823081 MS.gene067303:CDS
TTCTCGGCGCCACGGTTTAT+TGG 0.279842 8.2:-70824115 MS.gene067303:CDS
TCGGTTTATAACGGCGGAAT+TGG 0.290314 8.2:-70823580 MS.gene067303:CDS
GAATAGGTCGGGTAATTTAT+TGG 0.293961 8.2:-70823031 MS.gene067303:CDS
GCTGAGTATTCTGCGTTTAT+CGG 0.300204 8.2:+70824034 None:intergenic
ACAAATGCACCTGTTTGATT+TGG 0.324681 8.2:+70824074 None:intergenic
AACATTTGAAACGATGTTGA+TGG 0.338402 8.2:-70823874 MS.gene067303:CDS
AATGTTGATGGATGATTAAA+CGG 0.346660 8.2:+70823891 None:intergenic
TAAGGAATGTTAAGAGGAAT+AGG 0.348823 8.2:-70823047 MS.gene067303:CDS
AAACGGAGCTTCTTGTTATA+CGG 0.355478 8.2:-70823765 MS.gene067303:CDS
CCGGCGACGGTTACGGCGGC+GGG 0.362053 8.2:-70823552 MS.gene067303:CDS
ATAAAAGAATCGGTCGTGTT+TGG 0.378794 8.2:-70823788 MS.gene067303:CDS
TGCACCTGTTTGATTTGGTT+CGG 0.381073 8.2:+70824079 None:intergenic
GACTTCACCAATCTCATTAC+CGG 0.382732 8.2:-70824197 MS.gene067303:CDS
GAAAATCATTATTCTTTCTT+CGG 0.384048 8.2:+70823488 None:intergenic
ATTATTTCCGGTAATGAGAT+TGG 0.397632 8.2:+70824190 None:intergenic
CGGGCTATTAAATCGGTAAT+CGG 0.398376 8.2:-70823231 MS.gene067303:CDS
TCCGGCGACGGTTACGGCGG+CGG 0.401756 8.2:-70823553 MS.gene067303:CDS
GACGGTGATGGAAGAGGGTG+TGG 0.415506 8.2:-70823195 MS.gene067303:CDS
AACATCGTTTCAAATGTTGA+TGG 0.416178 8.2:+70823879 None:intergenic
GGTAATCGGGGCATTGCAAA+TGG 0.420117 8.2:-70823217 MS.gene067303:CDS
GTGGTGGTGGTGGAAAGAGG+TGG 0.420625 8.2:-70823923 MS.gene067303:CDS
CGACCCGGTCGGAGAAAATT+CGG 0.428033 8.2:+70823438 None:intergenic
ATCACCGGCGGTGGTGGTGG+TGG 0.432068 8.2:-70823933 MS.gene067303:CDS
GTGGTGGTGGAAAGAGGTGG+AGG 0.432428 8.2:-70823920 MS.gene067303:CDS
GTTAATATCACCGGCGGTGG+TGG 0.433518 8.2:-70823939 MS.gene067303:CDS
TTCTTGTTATACGGTGAATC+CGG 0.433520 8.2:-70823756 MS.gene067303:CDS
GGGCTATTAAATCGGTAATC+GGG 0.437429 8.2:-70823230 MS.gene067303:CDS
GGAATCTCCGGCGACGGTTA+CGG 0.440451 8.2:-70823559 MS.gene067303:CDS
TATGATCGAACGGCGGATAG+AGG 0.444092 8.2:-70823169 MS.gene067303:CDS
TTAGATTAAGGAATGTTAAG+AGG 0.444604 8.2:-70823053 MS.gene067303:CDS
GGTGGTCTTTGTGGTGGTGA+TGG 0.454004 8.2:-70823117 MS.gene067303:CDS
AGTTTGAGTCCGGCGGAGAT+AGG 0.458254 8.2:-70823288 MS.gene067303:CDS
AATATCACCGGCGGTGGTGG+TGG 0.459603 8.2:-70823936 MS.gene067303:CDS
CGGAAAATCTCCTCCACCGC+CGG 0.462392 8.2:+70823321 None:intergenic
GAATCGGTCGTGTTTGGAAA+CGG 0.462731 8.2:-70823782 MS.gene067303:CDS
ATCATCGAAGACAGGTTTCT+CGG 0.463592 8.2:-70824131 MS.gene067303:CDS
AAGAATTATTATAAAAGAAT+CGG 0.466313 8.2:-70823798 MS.gene067303:CDS
TCAACAACGATAATCGATTT+CGG 0.466829 8.2:+70823612 None:intergenic
GCCGAGTCGGGCTATTAAAT+CGG 0.467127 8.2:-70823238 MS.gene067303:CDS
GACTCAAACTCGCTCCCTGC+CGG 0.469388 8.2:+70823301 None:intergenic
AACGGCGGAATTGGAATCTC+CGG 0.478651 8.2:-70823571 MS.gene067303:CDS
ATCTAGCTTTGTTGCAGCCA+TGG 0.479264 8.2:-70823724 MS.gene067303:CDS
GAATCGGAACTCGCCGAGTC+GGG 0.479389 8.2:-70823250 MS.gene067303:CDS
GCTTTGGCGAATAGTTACTT+AGG 0.490135 8.2:-70823366 MS.gene067303:CDS
TTCGTTAATATCACCGGCGG+TGG 0.491350 8.2:-70823942 MS.gene067303:CDS
ACTTTACGATAGAACATGTT+CGG 0.492215 8.2:+70824260 None:intergenic
ATAGAACATGTTCGGTTGCA+TGG 0.499642 8.2:+70824268 None:intergenic
TTTCTACGGTTAAGGATTTG+AGG 0.500657 8.2:-70823092 MS.gene067303:CDS
AAAACCGAACCAAATCAAAC+AGG 0.507238 8.2:-70824083 MS.gene067303:CDS
GCGGTGGTGGTGGTGGAAAG+AGG 0.524660 8.2:-70823926 MS.gene067303:CDS
GCAGGGAGCGAGTTTGAGTC+CGG 0.529877 8.2:-70823298 MS.gene067303:CDS
CGGTGATGGAAGAGGGTGTG+GGG 0.533181 8.2:-70823193 MS.gene067303:CDS
GGCGGGGATACAGAATTTCA+TGG 0.536010 8.2:-70823535 MS.gene067303:CDS
GGATCTCGATCGGTTTATAA+CGG 0.539388 8.2:-70823589 MS.gene067303:CDS
CGTTTGTTCGTTAATATCAC+CGG 0.539498 8.2:-70823948 MS.gene067303:CDS
GAATGTTAAGAGGAATAGGT+CGG 0.544625 8.2:-70823043 MS.gene067303:CDS
ACGGTGATGGAAGAGGGTGT+GGG 0.545508 8.2:-70823194 MS.gene067303:CDS
ATATGAACGTCGACACGACC+CGG 0.546717 8.2:+70823423 None:intergenic
CCCGCCGCCGTAACCGTCGC+CGG 0.548081 8.2:+70823552 None:intergenic
CTGATCAAATCATCGAAGAC+AGG 0.551289 8.2:-70824139 MS.gene067303:CDS
GTGAGTTGATGATTGCGAAT+CGG 0.552462 8.2:-70823266 MS.gene067303:CDS
TCCGCTGAAATAGAATATAA+CGG 0.554085 8.2:-70823984 MS.gene067303:CDS
CGAATCGGAACTCGCCGAGT+CGG 0.559326 8.2:-70823251 MS.gene067303:CDS
ATATTCTATTTCAGCGGACA+TGG 0.564990 8.2:+70823989 None:intergenic
AGTAACCAAGGTGGTCTTTG+TGG 0.566453 8.2:-70823126 MS.gene067303:CDS
AATGTTAAGAGGAATAGGTC+GGG 0.568612 8.2:-70823042 MS.gene067303:CDS
GCCGTTATATTCTATTTCAG+CGG 0.568843 8.2:+70823983 None:intergenic
GAAATCGATTATCGTTGTTG+AGG 0.569205 8.2:-70823610 MS.gene067303:CDS
CTTTCCACCACCACCACCGC+CGG 0.576110 8.2:+70823929 None:intergenic
ATCAACTCACCTATCTCCGC+CGG 0.583450 8.2:+70823279 None:intergenic
TTATACGGTGAATCCGGTAC+CGG 0.585271 8.2:-70823750 MS.gene067303:CDS
AGACAGGTTTCTCGGCGCCA+CGG 0.586250 8.2:-70824123 MS.gene067303:CDS
ATCTCCGGCGACGGTTACGG+CGG 0.595968 8.2:-70823556 MS.gene067303:CDS
GCATTGCAAATGGACGGTGA+TGG 0.605343 8.2:-70823207 MS.gene067303:CDS
GGCTATTAAATCGGTAATCG+GGG 0.605421 8.2:-70823229 MS.gene067303:CDS
ATCGGGGCATTGCAAATGGA+CGG 0.606342 8.2:-70823213 MS.gene067303:CDS
AAGTCGGAATCTTCAAGAGA+AGG 0.608105 8.2:+70824215 None:intergenic
GGAATTGGAATCTCCGGCGA+CGG 0.612772 8.2:-70823565 MS.gene067303:CDS
GGGATACAGAATTTCATGGA+TGG 0.615467 8.2:-70823531 MS.gene067303:CDS
GGTAATGAGATTGGTGAAGT+CGG 0.621262 8.2:+70824199 None:intergenic
CAAATGGACGGTGATGGAAG+AGG 0.628389 8.2:-70823201 MS.gene067303:CDS
TTTATAGAACCAATAAACCG+TGG 0.631666 8.2:+70824106 None:intergenic
ACCGATTTAATAGCCCGACT+CGG 0.633107 8.2:+70823237 None:intergenic
TCGTTGTTGAGGATCTCGAT+CGG 0.655691 8.2:-70823599 MS.gene067303:CDS
ATAACAAAGAAAATTCGCCA+TGG 0.656962 8.2:+70823707 None:intergenic
CGGCGACGGTTACGGCGGCG+GGG 0.658490 8.2:-70823551 MS.gene067303:CDS
GAATAGTTACTTAGGAGTGA+AGG 0.659525 8.2:-70823358 MS.gene067303:CDS
AAATGGACGGTGATGGAAGA+GGG 0.660194 8.2:-70823200 MS.gene067303:CDS
GAACGTCGACACGACCCGGT+CGG 0.663610 8.2:+70823427 None:intergenic
CACCACCACAAAGACCACCT+TGG 0.666209 8.2:+70823121 None:intergenic
TTGTTCGTTAATATCACCGG+CGG 0.670649 8.2:-70823945 MS.gene067303:CDS
GGTGTGGGGATATGATCGAA+CGG 0.685070 8.2:-70823179 MS.gene067303:CDS
GCGGATAGAGGATGATGACG+TGG 0.692126 8.2:-70823157 MS.gene067303:CDS
TCTCGATCGGTTTATAACGG+CGG 0.701842 8.2:-70823586 MS.gene067303:CDS
GGGAGCGAGTTTGAGTCCGG+CGG 0.703907 8.2:-70823295 MS.gene067303:CDS
GTGGAAGATGAAAGTAACCA+AGG 0.706883 8.2:-70823138 MS.gene067303:CDS
AACCAAGGTGGTCTTTGTGG+TGG 0.706962 8.2:-70823123 MS.gene067303:CDS
GTGGGGATATGATCGAACGG+CGG 0.738828 8.2:-70823176 MS.gene067303:CDS
GAAGATGAAAGTAACCAAGG+TGG 0.754728 8.2:-70823135 MS.gene067303:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATTATTATAAAAGAAT+CGG - chr8.2:70823482-70823501 MS.gene067303:CDS 10.0%
!! GAAAATCATTATTCTTTCTT+CGG + chr8.2:70823795-70823814 None:intergenic 20.0%
!!! TTTATGGTTTGTTTAGATTA+AGG - chr8.2:70824215-70824234 MS.gene067303:CDS 20.0%
!!! TTTTTCATCTTTTAAAGCTT+TGG - chr8.2:70823898-70823917 MS.gene067303:CDS 20.0%
! TTAGATTAAGGAATGTTAAG+AGG - chr8.2:70824227-70824246 MS.gene067303:CDS 25.0%
!! AATGTTGATGGATGATTAAA+CGG + chr8.2:70823392-70823411 None:intergenic 25.0%
AAAGATGAAAAATCACACAC+CGG + chr8.2:70823890-70823909 None:intergenic 30.0%
AACATCGTTTCAAATGTTGA+TGG + chr8.2:70823404-70823423 None:intergenic 30.0%
AAGGATTTGAGGAAGATTTA+TGG - chr8.2:70824199-70824218 MS.gene067303:CDS 30.0%
ACTTTACGATAGAACATGTT+CGG + chr8.2:70823023-70823042 None:intergenic 30.0%
ATAACAAAGAAAATTCGCCA+TGG + chr8.2:70823576-70823595 None:intergenic 30.0%
ATTATTTCCGGTAATGAGAT+TGG + chr8.2:70823093-70823112 None:intergenic 30.0%
TAAGGAATGTTAAGAGGAAT+AGG - chr8.2:70824233-70824252 MS.gene067303:CDS 30.0%
TCCGCTGAAATAGAATATAA+CGG - chr8.2:70823296-70823315 MS.gene067303:CDS 30.0%
TTTATAGAACCAATAAACCG+TGG + chr8.2:70823177-70823196 None:intergenic 30.0%
! AACATTTGAAACGATGTTGA+TGG - chr8.2:70823406-70823425 MS.gene067303:CDS 30.0%
! TCAACAACGATAATCGATTT+CGG + chr8.2:70823671-70823690 None:intergenic 30.0%
!!! GATGTCGTTTTGATTATTTC+CGG + chr8.2:70823105-70823124 None:intergenic 30.0%
AAAACCGAACCAAATCAAAC+AGG - chr8.2:70823197-70823216 MS.gene067303:CDS 35.0%
AATGTTAAGAGGAATAGGTC+GGG - chr8.2:70824238-70824257 MS.gene067303:CDS 35.0%
ACAAATGCACCTGTTTGATT+TGG + chr8.2:70823209-70823228 None:intergenic 35.0%
ATAAAAGAATCGGTCGTGTT+TGG - chr8.2:70823492-70823511 MS.gene067303:CDS 35.0%
ATATTCTATTTCAGCGGACA+TGG + chr8.2:70823294-70823313 None:intergenic 35.0%
CGTTTGTTCGTTAATATCAC+CGG - chr8.2:70823332-70823351 MS.gene067303:CDS 35.0%
GAAATCGATTATCGTTGTTG+AGG - chr8.2:70823670-70823689 MS.gene067303:CDS 35.0%
GAATAGGTCGGGTAATTTAT+TGG - chr8.2:70824249-70824268 MS.gene067303:CDS 35.0%
GAATAGTTACTTAGGAGTGA+AGG - chr8.2:70823922-70823941 MS.gene067303:CDS 35.0%
GAATGTTAAGAGGAATAGGT+CGG - chr8.2:70824237-70824256 MS.gene067303:CDS 35.0%
GCCGTTATATTCTATTTCAG+CGG + chr8.2:70823300-70823319 None:intergenic 35.0%
TTTCTACGGTTAAGGATTTG+AGG - chr8.2:70824188-70824207 MS.gene067303:CDS 35.0%
! AAACGGAGCTTCTTGTTATA+CGG - chr8.2:70823515-70823534 MS.gene067303:CDS 35.0%
! CGACGTTCATATTCATTTTC+CGG - chr8.2:70823868-70823887 MS.gene067303:CDS 35.0%
! TGATGGTTTTTCTACGGTTA+AGG - chr8.2:70824180-70824199 MS.gene067303:CDS 35.0%
! TGCAACAAAGCTAGATTTTC+CGG + chr8.2:70823552-70823571 None:intergenic 35.0%
! TTCTTGTTATACGGTGAATC+CGG - chr8.2:70823524-70823543 MS.gene067303:CDS 35.0%
!! GAAGGAACACAAACTTTTTC+CGG - chr8.2:70823940-70823959 MS.gene067303:CDS 35.0%
AAGTCGGAATCTTCAAGAGA+AGG + chr8.2:70823068-70823087 None:intergenic 40.0%
ATCATCGAAGACAGGTTTCT+CGG - chr8.2:70823149-70823168 MS.gene067303:CDS 40.0%
CGGGCTATTAAATCGGTAAT+CGG - chr8.2:70824049-70824068 MS.gene067303:CDS 40.0%
CTGATCAAATCATCGAAGAC+AGG - chr8.2:70823141-70823160 MS.gene067303:CDS 40.0%
GAAGATGAAAGTAACCAAGG+TGG - chr8.2:70824145-70824164 MS.gene067303:CDS 40.0%
GACTTCACCAATCTCATTAC+CGG - chr8.2:70823083-70823102 MS.gene067303:CDS 40.0%
GCTGAGTATTCTGCGTTTAT+CGG + chr8.2:70823249-70823268 None:intergenic 40.0%
GCTTTGGCGAATAGTTACTT+AGG - chr8.2:70823914-70823933 MS.gene067303:CDS 40.0%
GGCTATTAAATCGGTAATCG+GGG - chr8.2:70824051-70824070 MS.gene067303:CDS 40.0%
GGGATACAGAATTTCATGGA+TGG - chr8.2:70823749-70823768 MS.gene067303:CDS 40.0%
GGGCTATTAAATCGGTAATC+GGG - chr8.2:70824050-70824069 MS.gene067303:CDS 40.0%
GGTAATGAGATTGGTGAAGT+CGG + chr8.2:70823084-70823103 None:intergenic 40.0%
GTGGAAGATGAAAGTAACCA+AGG - chr8.2:70824142-70824161 MS.gene067303:CDS 40.0%
TTGTTCGTTAATATCACCGG+CGG - chr8.2:70823335-70823354 MS.gene067303:CDS 40.0%
! AAAGCTAGATTTTCCGGTAC+CGG + chr8.2:70823546-70823565 None:intergenic 40.0%
! GGATCTCGATCGGTTTATAA+CGG - chr8.2:70823691-70823710 MS.gene067303:CDS 40.0%
!! GTGAGTTGATGATTGCGAAT+CGG - chr8.2:70824014-70824033 MS.gene067303:CDS 40.0%
!! TGCACCTGTTTGATTTGGTT+CGG + chr8.2:70823204-70823223 None:intergenic 40.0%
!!! TGGTGGTGATGGTTTTTCTA+CGG - chr8.2:70824174-70824193 MS.gene067303:CDS 40.0%
AGTAACCAAGGTGGTCTTTG+TGG - chr8.2:70824154-70824173 MS.gene067303:CDS 45.0%
GAATCGGTCGTGTTTGGAAA+CGG - chr8.2:70823498-70823517 MS.gene067303:CDS 45.0%
TCGGTTTATAACGGCGGAAT+TGG - chr8.2:70823700-70823719 MS.gene067303:CDS 45.0%
TCGTTGTTGAGGATCTCGAT+CGG - chr8.2:70823681-70823700 MS.gene067303:CDS 45.0%
TTATACGGTGAATCCGGTAC+CGG - chr8.2:70823530-70823549 MS.gene067303:CDS 45.0%
! ACCGATTTAATAGCCCGACT+CGG + chr8.2:70824046-70824065 None:intergenic 45.0%
! TCTCGATCGGTTTATAACGG+CGG - chr8.2:70823694-70823713 MS.gene067303:CDS 45.0%
! TGATCCGAATTTTCTCCGAC+CGG - chr8.2:70823838-70823857 MS.gene067303:CDS 45.0%
!! AAATGGACGGTGATGGAAGA+GGG - chr8.2:70824080-70824099 MS.gene067303:CDS 45.0%
!! ATCTAGCTTTGTTGCAGCCA+TGG - chr8.2:70823556-70823575 MS.gene067303:CDS 45.0%
!! GGAACACAAACTTTTTCCGG+CGG - chr8.2:70823943-70823962 MS.gene067303:CDS 45.0%
AACCAAGGTGGTCTTTGTGG+TGG - chr8.2:70824157-70824176 MS.gene067303:CDS 50.0%
AACGGCGGAATTGGAATCTC+CGG - chr8.2:70823709-70823728 MS.gene067303:CDS 50.0%
ATATGAACGTCGACACGACC+CGG + chr8.2:70823860-70823879 None:intergenic 50.0%
ATCAACTCACCTATCTCCGC+CGG + chr8.2:70824004-70824023 None:intergenic 50.0%
GCATTGCAAATGGACGGTGA+TGG - chr8.2:70824073-70824092 MS.gene067303:CDS 50.0%
GCCGAGTCGGGCTATTAAAT+CGG - chr8.2:70824042-70824061 MS.gene067303:CDS 50.0%
GGCGGGGATACAGAATTTCA+TGG - chr8.2:70823745-70823764 MS.gene067303:CDS 50.0%
GGTGTGGGGATATGATCGAA+CGG - chr8.2:70824101-70824120 MS.gene067303:CDS 50.0%
TATGATCGAACGGCGGATAG+AGG - chr8.2:70824111-70824130 MS.gene067303:CDS 50.0%
TTCGTTAATATCACCGGCGG+TGG - chr8.2:70823338-70823357 MS.gene067303:CDS 50.0%
! GATCCGAATTTTCTCCGACC+GGG - chr8.2:70823839-70823858 MS.gene067303:CDS 50.0%
!! ACACAAACTTTTTCCGGCGG+TGG - chr8.2:70823946-70823965 MS.gene067303:CDS 50.0%
!! ATCGGGGCATTGCAAATGGA+CGG - chr8.2:70824067-70824086 MS.gene067303:CDS 50.0%
!! CAAATGGACGGTGATGGAAG+AGG - chr8.2:70824079-70824098 MS.gene067303:CDS 50.0%
!! GGTAATCGGGGCATTGCAAA+TGG - chr8.2:70824063-70824082 MS.gene067303:CDS 50.0%
AGTTTGAGTCCGGCGGAGAT+AGG - chr8.2:70823992-70824011 MS.gene067303:CDS 55.0%
CACCACCACAAAGACCACCT+TGG + chr8.2:70824162-70824181 None:intergenic 55.0%
CGACCCGGTCGGAGAAAATT+CGG + chr8.2:70823845-70823864 None:intergenic 55.0%
GGAATTGGAATCTCCGGCGA+CGG - chr8.2:70823715-70823734 MS.gene067303:CDS 55.0%
GTGGGGATATGATCGAACGG+CGG - chr8.2:70824104-70824123 MS.gene067303:CDS 55.0%
GTTAATATCACCGGCGGTGG+TGG - chr8.2:70823341-70823360 MS.gene067303:CDS 55.0%
TTCTCGGCGCCACGGTTTAT+TGG - chr8.2:70823165-70823184 MS.gene067303:CDS 55.0%
! GCGGATAGAGGATGATGACG+TGG - chr8.2:70824123-70824142 MS.gene067303:CDS 55.0%
! GGTGGTCTTTGTGGTGGTGA+TGG - chr8.2:70824163-70824182 MS.gene067303:CDS 55.0%
! GTGGAGGAGATTTTCCGGCA+GGG - chr8.2:70823965-70823984 MS.gene067303:CDS 55.0%
!! ACGGTGATGGAAGAGGGTGT+GGG - chr8.2:70824086-70824105 MS.gene067303:CDS 55.0%
!! CAAACTTTTTCCGGCGGTGG+AGG - chr8.2:70823949-70823968 MS.gene067303:CDS 55.0%
AGACAGGTTTCTCGGCGCCA+CGG - chr8.2:70823157-70823176 MS.gene067303:CDS 60.0%
CGAATCGGAACTCGCCGAGT+CGG - chr8.2:70824029-70824048 MS.gene067303:CDS 60.0%
CGGAAAATCTCCTCCACCGC+CGG + chr8.2:70823962-70823981 None:intergenic 60.0%
GAATCGGAACTCGCCGAGTC+GGG - chr8.2:70824030-70824049 MS.gene067303:CDS 60.0%
GACTCAAACTCGCTCCCTGC+CGG + chr8.2:70823982-70824001 None:intergenic 60.0%
GCAGGGAGCGAGTTTGAGTC+CGG - chr8.2:70823982-70824001 MS.gene067303:CDS 60.0%
GGAATCTCCGGCGACGGTTA+CGG - chr8.2:70823721-70823740 MS.gene067303:CDS 60.0%
! AATATCACCGGCGGTGGTGG+TGG - chr8.2:70823344-70823363 MS.gene067303:CDS 60.0%
! CGGCGGTGGAGGAGATTTTC+CGG - chr8.2:70823960-70823979 MS.gene067303:CDS 60.0%
! GGTGGAGGAGATTTTCCGGC+AGG - chr8.2:70823964-70823983 MS.gene067303:CDS 60.0%
!! CGGTGATGGAAGAGGGTGTG+GGG - chr8.2:70824087-70824106 MS.gene067303:CDS 60.0%
!! GACGGTGATGGAAGAGGGTG+TGG - chr8.2:70824085-70824104 MS.gene067303:CDS 60.0%
!! GTGGTGGTGGAAAGAGGTGG+AGG - chr8.2:70823360-70823379 MS.gene067303:CDS 60.0%
!! GTGGTGGTGGTGGAAAGAGG+TGG - chr8.2:70823357-70823376 MS.gene067303:CDS 60.0%
ATCTCCGGCGACGGTTACGG+CGG - chr8.2:70823724-70823743 MS.gene067303:CDS 65.0%
CTTTCCACCACCACCACCGC+CGG + chr8.2:70823354-70823373 None:intergenic 65.0%
GAACGTCGACACGACCCGGT+CGG + chr8.2:70823856-70823875 None:intergenic 65.0%
GGGAGCGAGTTTGAGTCCGG+CGG - chr8.2:70823985-70824004 MS.gene067303:CDS 65.0%
!! GCGGTGGTGGTGGTGGAAAG+AGG - chr8.2:70823354-70823373 MS.gene067303:CDS 65.0%
!! ATCACCGGCGGTGGTGGTGG+TGG - chr8.2:70823347-70823366 MS.gene067303:CDS 70.0%
TCCGGCGACGGTTACGGCGG+CGG - chr8.2:70823727-70823746 MS.gene067303:CDS 75.0%
! CCCGCCGCCGTAACCGTCGC+CGG + chr8.2:70823731-70823750 None:intergenic 80.0%
! CCGGCGACGGTTACGGCGGC+GGG - chr8.2:70823728-70823747 MS.gene067303:CDS 80.0%
! CGGCGACGGTTACGGCGGCG+GGG - chr8.2:70823729-70823748 MS.gene067303:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 70823014 70824288 70823014 ID=MS.gene067303
chr8.2 mRNA 70823014 70824288 70823014 ID=MS.gene067303.t1;Parent=MS.gene067303
chr8.2 exon 70823014 70824288 70823014 ID=MS.gene067303.t1.exon1;Parent=MS.gene067303.t1
chr8.2 CDS 70823014 70824288 70823014 ID=cds.MS.gene067303.t1;Parent=MS.gene067303.t1
Gene Sequence

>MS.gene067303

ATGCAACCGAACATGTTCTATCGTAAAGTTTCGCTTTATTTACATTCTCTTCCTTCTCTTGAAGATTCCGACTTCACCAATCTCATTACCGGAAATAATCAAAACGACATCGTTTTAAAGCTAGACTCTGATCAAATCATCGAAGACAGGTTTCTCGGCGCCACGGTTTATTGGTTCTATAAAAAAACCGAACCAAATCAAACAGGTGCATTTGTTCTTAAGATCAGAAAAACCGATAAACGCAGAATACTCAGCAGTTATCTTCATCATATTACTACCATGTCCGCTGAAATAGAATATAACGGCAAACGTGATTTACGTTTGTTCGTTAATATCACCGGCGGTGGTGGTGGTGGAAAGAGGTGGAGGTCTATTCCGTTTAATCATCCATCAACATTTGAAACGATGTTGATGGAAACTGATTTGAAGAACAAAATCAAAAACGATCTCGAATCGTTTTTGAAAGCGAAGAATTATTATAAAAGAATCGGTCGTGTTTGGAAACGGAGCTTCTTGTTATACGGTGAATCCGGTACCGGAAAATCTAGCTTTGTTGCAGCCATGGCGAATTTTCTTTGTTATGATGTATATGATGTTGATCTCTCAAAAATTCAAAGTGATTCAGATTTGAAATTTCTCTTACTAGAAACATCACCGAAATCGATTATCGTTGTTGAGGATCTCGATCGGTTTATAACGGCGGAATTGGAATCTCCGGCGACGGTTACGGCGGCGGGGATACAGAATTTCATGGATGGAGTTATGACTTCGAGTTACGCCGAAGAAAGAATAATGATTTTCACGATGAATAGTAAAGAATTTATTGATCCGAATTTTCTCCGACCGGGTCGTGTCGACGTTCATATTCATTTTCCGGTGTGTGATTTTTCATCTTTTAAAGCTTTGGCGAATAGTTACTTAGGAGTGAAGGAACACAAACTTTTTCCGGCGGTGGAGGAGATTTTCCGGCAGGGAGCGAGTTTGAGTCCGGCGGAGATAGGTGAGTTGATGATTGCGAATCGGAACTCGCCGAGTCGGGCTATTAAATCGGTAATCGGGGCATTGCAAATGGACGGTGATGGAAGAGGGTGTGGGGATATGATCGAACGGCGGATAGAGGATGATGACGTGGAAGATGAAAGTAACCAAGGTGGTCTTTGTGGTGGTGATGGTTTTTCTACGGTTAAGGATTTGAGGAAGATTTATGGTTTGTTTAGATTAAGGAATGTTAAGAGGAATAGGTCGGGTAATTTATTGGTCAATGATGAAAGATAA
Protein sequence

>MS.gene067303.t1

MQPNMFYRKVSLYLHSLPSLEDSDFTNLITGNNQNDIVLKLDSDQIIEDRFLGATVYWFYKKTEPNQTGAFVLKIRKTDKRRILSSYLHHITTMSAEIEYNGKRDLRLFVNITGGGGGGKRWRSIPFNHPSTFETMLMETDLKNKIKNDLESFLKAKNYYKRIGRVWKRSFLLYGESGTGKSSFVAAMANFLCYDVYDVDLSKIQSDSDLKFLLLETSPKSIIVVEDLDRFITAELESPATVTAAGIQNFMDGVMTSSYAEERIMIFTMNSKEFIDPNFLRPGRVDVHIHFPVCDFSSFKALANSYLGVKEHKLFPAVEEIFRQGASLSPAEIGELMIANRNSPSRAIKSVIGALQMDGDGRGCGDMIERRIEDDDVEDESNQGGLCGGDGFSTVKDLRKIYGLFRLRNVKRNRSGNLLVNDER