Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060518.t1 | XP_024629354.1 | 95.1 | 425 | 19 | 1 | 1 | 423 | 59 | 483 | 1.00E-228 | 802.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060518.t1 | F4IJ77 | 57.5 | 428 | 161 | 7 | 1 | 413 | 60 | 481 | 1.9e-130 | 467.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060518.t1 | A0A396GJ98 | 95.1 | 425 | 19 | 1 | 1 | 423 | 59 | 483 | 7.5e-229 | 802.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060518 | MS.gene067303 | 0.807247 | 5.55E-50 | -1.69E-46 |
| MS.gene060518 | MS.gene072153 | 0.801903 | 7.23E-49 | -1.69E-46 |
| MS.gene060518 | MS.gene36571 | 0.805612 | 1.23E-49 | -1.69E-46 |
| MS.gene060518 | MS.gene44909 | 0.801595 | 8.36E-49 | -1.69E-46 |
| MS.gene060518 | MS.gene84729 | 0.936279 | 2.24E-97 | -1.69E-46 |
| MS.gene060518 | MS.gene84862 | 0.95683 | 1.17E-114 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060518.t1 | MTR_8g027200 | 89.412 | 425 | 17 | 2 | 1 | 423 | 59 | 457 | 0.0 | 759 |
| MS.gene060518.t1 | MTR_7g117950 | 63.373 | 415 | 128 | 9 | 1 | 414 | 61 | 452 | 0.0 | 520 |
| MS.gene060518.t1 | MTR_3g092060 | 52.745 | 419 | 174 | 9 | 4 | 412 | 66 | 470 | 8.37e-154 | 444 |
| MS.gene060518.t1 | MTR_2g061820 | 36.410 | 390 | 212 | 5 | 1 | 356 | 57 | 444 | 1.07e-86 | 273 |
| MS.gene060518.t1 | MTR_4g128260 | 34.048 | 420 | 224 | 9 | 1 | 371 | 59 | 474 | 7.99e-72 | 235 |
| MS.gene060518.t1 | MTR_8g076920 | 35.635 | 362 | 200 | 9 | 4 | 338 | 81 | 436 | 6.91e-68 | 224 |
| MS.gene060518.t1 | MTR_5g020980 | 35.393 | 356 | 199 | 5 | 34 | 360 | 100 | 453 | 4.66e-66 | 219 |
| MS.gene060518.t1 | MTR_1g022250 | 34.264 | 394 | 206 | 10 | 7 | 357 | 69 | 452 | 9.48e-66 | 218 |
| MS.gene060518.t1 | MTR_2g076690 | 35.965 | 342 | 183 | 10 | 41 | 356 | 104 | 435 | 2.59e-65 | 216 |
| MS.gene060518.t1 | MTR_2g030420 | 33.419 | 389 | 211 | 8 | 7 | 357 | 72 | 450 | 7.99e-65 | 215 |
| MS.gene060518.t1 | MTR_6g009720 | 33.684 | 380 | 197 | 7 | 7 | 335 | 71 | 446 | 1.74e-64 | 216 |
| MS.gene060518.t1 | MTR_2g076840 | 32.199 | 382 | 220 | 10 | 1 | 356 | 58 | 426 | 5.17e-64 | 219 |
| MS.gene060518.t1 | MTR_2g076840 | 41.935 | 248 | 129 | 6 | 122 | 357 | 460 | 704 | 4.80e-58 | 202 |
| MS.gene060518.t1 | MTR_6g009520 | 32.405 | 395 | 220 | 10 | 7 | 357 | 71 | 462 | 1.42e-63 | 213 |
| MS.gene060518.t1 | MTR_2g076970 | 36.824 | 296 | 170 | 5 | 70 | 356 | 141 | 428 | 1.91e-63 | 211 |
| MS.gene060518.t1 | MTR_1g022245 | 33.903 | 351 | 180 | 6 | 34 | 335 | 91 | 438 | 2.14e-63 | 212 |
| MS.gene060518.t1 | MTR_7g008040 | 34.133 | 375 | 197 | 9 | 29 | 356 | 89 | 460 | 2.57e-63 | 213 |
| MS.gene060518.t1 | MTR_4g107370 | 33.773 | 379 | 194 | 8 | 31 | 355 | 95 | 470 | 4.92e-63 | 212 |
| MS.gene060518.t1 | MTR_2g076710 | 34.311 | 341 | 195 | 8 | 41 | 356 | 102 | 438 | 1.25e-62 | 209 |
| MS.gene060518.t1 | MTR_6g009770 | 31.605 | 405 | 219 | 8 | 7 | 357 | 48 | 448 | 1.58e-62 | 210 |
| MS.gene060518.t1 | MTR_6g009553 | 31.695 | 407 | 218 | 8 | 7 | 357 | 47 | 449 | 1.82e-62 | 209 |
| MS.gene060518.t1 | MTR_6g009540 | 31.671 | 401 | 227 | 9 | 1 | 357 | 63 | 460 | 1.86e-62 | 210 |
| MS.gene060518.t1 | MTR_2g076920 | 34.670 | 349 | 193 | 9 | 33 | 356 | 90 | 428 | 2.04e-62 | 209 |
| MS.gene060518.t1 | MTR_5g020990 | 33.875 | 369 | 186 | 9 | 34 | 356 | 114 | 470 | 8.52e-62 | 207 |
| MS.gene060518.t1 | MTR_2g076900 | 32.665 | 349 | 206 | 7 | 33 | 356 | 89 | 433 | 3.36e-60 | 202 |
| MS.gene060518.t1 | MTR_2g076990 | 29.095 | 409 | 241 | 9 | 1 | 382 | 1 | 387 | 4.18e-59 | 199 |
| MS.gene060518.t1 | MTR_6g042290 | 34.091 | 352 | 205 | 8 | 7 | 335 | 81 | 428 | 5.95e-59 | 200 |
| MS.gene060518.t1 | MTR_3g103150 | 39.357 | 249 | 134 | 6 | 122 | 356 | 31 | 276 | 1.01e-57 | 191 |
| MS.gene060518.t1 | MTR_6g009510 | 31.622 | 370 | 201 | 8 | 1 | 356 | 75 | 406 | 2.94e-54 | 187 |
| MS.gene060518.t1 | MTR_6g009660 | 28.686 | 373 | 192 | 10 | 29 | 357 | 70 | 412 | 2.93e-44 | 160 |
| MS.gene060518.t1 | MTR_5g021020 | 31.629 | 313 | 177 | 13 | 56 | 356 | 118 | 405 | 1.91e-39 | 146 |
| MS.gene060518.t1 | MTR_5g020720 | 37.415 | 147 | 77 | 4 | 191 | 324 | 25 | 169 | 4.01e-21 | 90.9 |
| MS.gene060518.t1 | MTR_5g021000 | 38.889 | 108 | 61 | 4 | 251 | 356 | 1 | 105 | 7.11e-18 | 81.3 |
| MS.gene060518.t1 | MTR_1g011680 | 35.417 | 96 | 60 | 2 | 240 | 335 | 18 | 111 | 1.42e-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060518.t1 | AT2G46620 | 57.437 | 437 | 165 | 7 | 1 | 423 | 60 | 489 | 1.23e-164 | 472 |
| MS.gene060518.t1 | AT4G25835 | 35.581 | 430 | 221 | 10 | 1 | 384 | 59 | 478 | 1.57e-75 | 244 |
| MS.gene060518.t1 | AT5G57480 | 35.202 | 446 | 218 | 10 | 4 | 399 | 62 | 486 | 2.17e-74 | 241 |
| MS.gene060518.t1 | AT5G17760 | 41.391 | 302 | 151 | 8 | 71 | 356 | 163 | 454 | 2.32e-69 | 228 |
| MS.gene060518.t1 | AT3G28580 | 36.182 | 351 | 180 | 7 | 30 | 338 | 91 | 439 | 1.87e-68 | 225 |
| MS.gene060518.t1 | AT4G30250 | 34.750 | 400 | 212 | 8 | 4 | 356 | 62 | 459 | 4.62e-67 | 222 |
| MS.gene060518.t1 | AT4G30250 | 34.750 | 400 | 212 | 8 | 4 | 356 | 64 | 461 | 5.09e-67 | 222 |
| MS.gene060518.t1 | AT5G40010 | 35.230 | 369 | 189 | 9 | 26 | 348 | 90 | 454 | 5.31e-67 | 222 |
| MS.gene060518.t1 | AT2G18193 | 38.922 | 334 | 173 | 9 | 34 | 343 | 101 | 427 | 1.57e-66 | 220 |
| MS.gene060518.t1 | AT3G50930 | 35.933 | 359 | 189 | 10 | 34 | 357 | 148 | 500 | 2.62e-66 | 222 |
| MS.gene060518.t1 | AT3G28580 | 36.280 | 328 | 189 | 6 | 30 | 338 | 91 | 417 | 1.62e-65 | 217 |
| MS.gene060518.t1 | AT3G28600 | 37.176 | 347 | 184 | 11 | 37 | 356 | 107 | 446 | 2.13e-65 | 217 |
| MS.gene060518.t1 | AT3G28610 | 37.425 | 334 | 175 | 10 | 37 | 343 | 106 | 432 | 2.70e-65 | 216 |
| MS.gene060518.t1 | AT3G50940 | 35.758 | 330 | 180 | 7 | 34 | 338 | 102 | 424 | 9.67e-65 | 214 |
| MS.gene060518.t1 | AT5G17750 | 36.364 | 341 | 179 | 7 | 13 | 322 | 57 | 390 | 2.09e-64 | 212 |
| MS.gene060518.t1 | AT2G18190 | 36.474 | 329 | 181 | 7 | 34 | 338 | 102 | 426 | 8.81e-64 | 213 |
| MS.gene060518.t1 | AT5G17740 | 31.765 | 425 | 230 | 13 | 3 | 390 | 71 | 472 | 1.53e-63 | 213 |
| MS.gene060518.t1 | AT5G17730 | 37.037 | 297 | 166 | 7 | 73 | 356 | 155 | 443 | 1.29e-62 | 209 |
| MS.gene060518.t1 | AT5G40000 | 35.652 | 345 | 191 | 11 | 37 | 356 | 111 | 449 | 3.29e-61 | 206 |
| MS.gene060518.t1 | AT3G28540 | 33.626 | 342 | 185 | 7 | 33 | 335 | 96 | 434 | 8.24e-61 | 206 |
| MS.gene060518.t1 | AT3G28540 | 33.626 | 342 | 185 | 7 | 33 | 335 | 96 | 434 | 8.59e-61 | 206 |
| MS.gene060518.t1 | AT3G28510 | 32.970 | 367 | 200 | 8 | 33 | 356 | 95 | 458 | 8.88e-61 | 206 |
| MS.gene060518.t1 | AT4G05380 | 37.500 | 336 | 176 | 8 | 47 | 356 | 120 | 447 | 1.45e-60 | 204 |
| MS.gene060518.t1 | AT3G28600 | 39.928 | 278 | 140 | 7 | 104 | 356 | 132 | 407 | 1.05e-59 | 201 |
| MS.gene060518.t1 | AT3G28570 | 33.807 | 352 | 210 | 9 | 22 | 356 | 88 | 433 | 4.67e-59 | 199 |
| MS.gene060518.t1 | AT3G28570 | 33.807 | 352 | 210 | 9 | 22 | 356 | 95 | 440 | 5.78e-59 | 199 |
| MS.gene060518.t1 | AT1G43910 | 33.951 | 324 | 180 | 7 | 47 | 343 | 119 | 435 | 1.93e-53 | 185 |
| MS.gene060518.t1 | AT3G28520 | 32.945 | 343 | 188 | 8 | 33 | 338 | 85 | 422 | 6.44e-52 | 181 |
| MS.gene060518.t1 | AT5G17760 | 48.428 | 159 | 75 | 2 | 71 | 229 | 163 | 314 | 2.13e-40 | 147 |
| MS.gene060518.t1 | AT3G29800 | 28.875 | 329 | 205 | 11 | 27 | 342 | 55 | 367 | 8.41e-37 | 139 |
| MS.gene060518.t1 | AT4G05340 | 47.826 | 69 | 35 | 1 | 242 | 310 | 27 | 94 | 2.06e-13 | 66.2 |
Find 97 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATGGTTTGTTTAGATTA+AGG | 0.215457 | 8.1:-75237050 | MS.gene060518:CDS |
| ACGGATTTGAGGAAGATTTA+TGG | 0.228137 | 8.1:-75237066 | MS.gene060518:CDS |
| TTCTCGGCGCCACGGTTTAT+TGG | 0.279842 | 8.1:-75238094 | MS.gene060518:CDS |
| GAATAGGTCGGGTAATTTAT+TGG | 0.293961 | 8.1:-75237016 | MS.gene060518:CDS |
| GCTGAGTATTCTGCGTTTAT+CGG | 0.300204 | 8.1:+75238013 | None:intergenic |
| GGGGTGGTGGAAGGAGGTGG+AGG | 0.315568 | 8.1:-75237899 | MS.gene060518:CDS |
| ACAAATGCACCTGTTTGATT+TGG | 0.324681 | 8.1:+75238053 | None:intergenic |
| TCGGTTTATAACATCGGAAT+TGG | 0.335050 | 8.1:-75237559 | MS.gene060518:CDS |
| AACATTTGAAACGATGTTGA+TGG | 0.338402 | 8.1:-75237853 | MS.gene060518:CDS |
| AATGTTGATGGATGATTAAA+CGG | 0.346660 | 8.1:+75237870 | None:intergenic |
| TAAGGAATGTTAAGAGGAAT+AGG | 0.348823 | 8.1:-75237032 | MS.gene060518:CDS |
| CCGGCAACGGTTACGGCGGC+GGG | 0.352366 | 8.1:-75237531 | MS.gene060518:CDS |
| AAACGGAGCTTCTTGTTATA+CGG | 0.355478 | 8.1:-75237744 | MS.gene060518:CDS |
| ATAAAAGAATCGGTCGTGTT+TGG | 0.378794 | 8.1:-75237767 | MS.gene060518:CDS |
| TGCACCTGTTTGATTTGGTT+CGG | 0.381073 | 8.1:+75238058 | None:intergenic |
| GACTTCACCAATCTCATTAC+CGG | 0.382732 | 8.1:-75238176 | MS.gene060518:CDS |
| GAAAATCATTATTCTTTCTT+CGG | 0.384048 | 8.1:+75237467 | None:intergenic |
| ATTATTTCCGGTAATGAGAT+TGG | 0.397632 | 8.1:+75238169 | None:intergenic |
| CGGGCTATTAAATCGGTAAT+CGG | 0.398376 | 8.1:-75237210 | MS.gene060518:CDS |
| CCCGCCGCCGTAACCGTTGC+CGG | 0.398549 | 8.1:+75237531 | None:intergenic |
| TCCGGCAACGGTTACGGCGG+CGG | 0.415110 | 8.1:-75237532 | MS.gene060518:CDS |
| CGACCAGGTCGGAGAAAATT+CGG | 0.415681 | 8.1:+75237417 | None:intergenic |
| AACATCGTTTCAAATGTTGA+TGG | 0.416178 | 8.1:+75237858 | None:intergenic |
| TTCGTTAATATCACCGGCGG+TGG | 0.419095 | 8.1:-75237921 | MS.gene060518:CDS |
| GGTAATCGGGGCATTGCAAA+TGG | 0.420117 | 8.1:-75237196 | MS.gene060518:CDS |
| GTTAATATCACCGGCGGTGG+GGG | 0.422663 | 8.1:-75237918 | MS.gene060518:CDS |
| TCAACAACGATAATAGATTT+CGG | 0.429950 | 8.1:+75237591 | None:intergenic |
| GGAATCTCCGGCAACGGTTA+CGG | 0.431889 | 8.1:-75237538 | MS.gene060518:CDS |
| TTCTTGTTATACGGTGAATC+CGG | 0.433520 | 8.1:-75237735 | MS.gene060518:CDS |
| GGGCTATTAAATCGGTAATC+GGG | 0.437429 | 8.1:-75237209 | MS.gene060518:CDS |
| TTAGATTAAGGAATGTTAAG+AGG | 0.444604 | 8.1:-75237038 | MS.gene060518:CDS |
| AACATCGGAATTGGAATCTC+CGG | 0.452087 | 8.1:-75237550 | MS.gene060518:CDS |
| GGTGTTCTTTGTGGTGGTGA+TGG | 0.454004 | 8.1:-75237096 | MS.gene060518:CDS |
| AGTTTGAGTCCGGCGGAGAT+AGG | 0.458254 | 8.1:-75237267 | MS.gene060518:CDS |
| CGGAAAATCTCCTCCACCGC+CGG | 0.462392 | 8.1:+75237300 | None:intergenic |
| GAATCGGTCGTGTTTGGAAA+CGG | 0.462731 | 8.1:-75237761 | MS.gene060518:CDS |
| ATCATCGAAGACAGGTTTCT+CGG | 0.463592 | 8.1:-75238110 | MS.gene060518:CDS |
| GACGGTGATGGAAAAGGGTG+TGG | 0.465085 | 8.1:-75237174 | MS.gene060518:CDS |
| AAGAATTATTATAAAAGAAT+CGG | 0.466313 | 8.1:-75237777 | MS.gene060518:CDS |
| GCCGAGTCGGGCTATTAAAT+CGG | 0.467127 | 8.1:-75237217 | MS.gene060518:CDS |
| GACTCAAACTCGCTCCCTGC+CGG | 0.469388 | 8.1:+75237280 | None:intergenic |
| TCGTTAATATCACCGGCGGT+GGG | 0.469999 | 8.1:-75237920 | MS.gene060518:CDS |
| TATGATCGTACGGCGGATAG+AGG | 0.472611 | 8.1:-75237148 | MS.gene060518:CDS |
| ATCTAGCTTTGTTGCAGCCA+TGG | 0.479264 | 8.1:-75237703 | MS.gene060518:CDS |
| GAATCGGAACTCGCCGAGTC+GGG | 0.479389 | 8.1:-75237229 | MS.gene060518:CDS |
| AATTTATTGGTCAATGATGA+AGG | 0.489531 | 8.1:-75237003 | MS.gene060518:CDS |
| ACTTTACGATAGAACATGTT+CGG | 0.492215 | 8.1:+75238239 | None:intergenic |
| GCTTTGGCGAATAGTTACTT+AGG | 0.495433 | 8.1:-75237345 | MS.gene060518:CDS |
| ATAGAACATGTTCGGTTGCA+TGG | 0.499642 | 8.1:+75238247 | None:intergenic |
| CGTTAATATCACCGGCGGTG+GGG | 0.505377 | 8.1:-75237919 | MS.gene060518:CDS |
| AAAACCGAACCAAATCAAAC+AGG | 0.507238 | 8.1:-75238062 | MS.gene060518:CDS |
| ACGGTGATGGAAAAGGGTGT+GGG | 0.516936 | 8.1:-75237173 | MS.gene060518:CDS |
| GGCGGGGATTCAGAATTTCA+TGG | 0.525375 | 8.1:-75237514 | MS.gene060518:CDS |
| ATATGAACGTCCACACGACC+AGG | 0.525421 | 8.1:+75237402 | None:intergenic |
| GCAGGGAGCGAGTTTGAGTC+CGG | 0.529877 | 8.1:-75237277 | MS.gene060518:CDS |
| AAGTCGGAATCTTCAAAAGA+AGG | 0.531359 | 8.1:+75238194 | None:intergenic |
| CGTTTGTTCGTTAATATCAC+CGG | 0.539498 | 8.1:-75237927 | MS.gene060518:CDS |
| GAAATCTATTATCGTTGTTG+AGG | 0.540126 | 8.1:-75237589 | MS.gene060518:CDS |
| GAATGTTAAGAGGAATAGGT+CGG | 0.544625 | 8.1:-75237028 | MS.gene060518:CDS |
| GTGAGTTGATGATTGCGAAT+CGG | 0.552462 | 8.1:-75237245 | MS.gene060518:CDS |
| CGAATCGGAACTCGCCGAGT+CGG | 0.559326 | 8.1:-75237230 | MS.gene060518:CDS |
| GCCGTTGTATTCTATTTCAG+CGG | 0.560252 | 8.1:+75237962 | None:intergenic |
| CCGATCAAATCATCGAAGAC+AGG | 0.562107 | 8.1:-75238118 | MS.gene060518:CDS |
| GTATTCTATTTCAGCGGACA+TGG | 0.562971 | 8.1:+75237968 | None:intergenic |
| CGGTGATGGAAAAGGGTGTG+GGG | 0.563898 | 8.1:-75237172 | MS.gene060518:CDS |
| AATGTTAAGAGGAATAGGTC+GGG | 0.568612 | 8.1:-75237027 | MS.gene060518:CDS |
| CCTGTCTTCGATGATTTGAT+CGG | 0.569361 | 8.1:+75238118 | None:intergenic |
| CAAATGGACGGTGATGGAAA+AGG | 0.573028 | 8.1:-75237180 | MS.gene060518:CDS |
| ATCAACTCACCTATCTCCGC+CGG | 0.583450 | 8.1:+75237258 | None:intergenic |
| TTATACGGTGAATCCGGTAC+CGG | 0.585271 | 8.1:-75237729 | MS.gene060518:CDS |
| AGACAGGTTTCTCGGCGCCA+CGG | 0.586250 | 8.1:-75238102 | MS.gene060518:CDS |
| AGTAACCAAGGTGTTCTTTG+TGG | 0.595481 | 8.1:-75237105 | MS.gene060518:CDS |
| GCATTGCAAATGGACGGTGA+TGG | 0.605343 | 8.1:-75237186 | MS.gene060518:CDS |
| GGCTATTAAATCGGTAATCG+GGG | 0.605421 | 8.1:-75237208 | MS.gene060518:CDS |
| ATCGGGGCATTGCAAATGGA+CGG | 0.606342 | 8.1:-75237192 | MS.gene060518:CDS |
| AAATGGACGGTGATGGAAAA+GGG | 0.611545 | 8.1:-75237179 | MS.gene060518:CDS |
| ATCTCCGGCAACGGTTACGG+CGG | 0.614797 | 8.1:-75237535 | MS.gene060518:CDS |
| TCTCGATCGGTTTATAACAT+CGG | 0.620044 | 8.1:-75237565 | MS.gene060518:CDS |
| GGTAATGAGATTGGTGAAGT+CGG | 0.621262 | 8.1:+75238178 | None:intergenic |
| GGGATTCAGAATTTCATGGA+CGG | 0.622282 | 8.1:-75237510 | MS.gene060518:CDS |
| GGAATTGGAATCTCCGGCAA+CGG | 0.626385 | 8.1:-75237544 | MS.gene060518:CDS |
| TTTATAGAACCAATAAACCG+TGG | 0.631666 | 8.1:+75238085 | None:intergenic |
| ACCGATTTAATAGCCCGACT+CGG | 0.633107 | 8.1:+75237216 | None:intergenic |
| GAACGTCCACACGACCAGGT+CGG | 0.633799 | 8.1:+75237406 | None:intergenic |
| AACCAAGGTGTTCTTTGTGG+TGG | 0.636999 | 8.1:-75237102 | MS.gene060518:CDS |
| CGGCAACGGTTACGGCGGCG+GGG | 0.642529 | 8.1:-75237530 | MS.gene060518:CDS |
| GGTGTGGGGATATGATCGTA+CGG | 0.644792 | 8.1:-75237158 | MS.gene060518:CDS |
| TCGTTGTTGAGGATCTCGAT+CGG | 0.655691 | 8.1:-75237578 | MS.gene060518:CDS |
| ATAACAAAGAAAATTCGCCA+TGG | 0.656962 | 8.1:+75237686 | None:intergenic |
| GAATAGTTACTTAGGTGTGA+AGG | 0.666896 | 8.1:-75237337 | MS.gene060518:CDS |
| TTGTTCGTTAATATCACCGG+CGG | 0.670649 | 8.1:-75237924 | MS.gene060518:CDS |
| CACCACCACAAAGAACACCT+TGG | 0.671779 | 8.1:+75237100 | None:intergenic |
| GCGGATAGAGGATGATGACG+TGG | 0.692126 | 8.1:-75237136 | MS.gene060518:CDS |
| GTGGAAGATGAAAGTAACCA+AGG | 0.702124 | 8.1:-75237117 | MS.gene060518:CDS |
| GGGAGCGAGTTTGAGTCCGG+CGG | 0.703907 | 8.1:-75237274 | MS.gene060518:CDS |
| TCCGCTGAAATAGAATACAA+CGG | 0.706310 | 8.1:-75237963 | MS.gene060518:CDS |
| GTGGGGATATGATCGTACGG+CGG | 0.779328 | 8.1:-75237155 | MS.gene060518:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAATTATTATAAAAGAAT+CGG | - | chr8.1:75237464-75237483 | MS.gene060518:CDS | 10.0% |
| !! | GAAAATCATTATTCTTTCTT+CGG | + | chr8.1:75237777-75237796 | None:intergenic | 20.0% |
| !!! | TTTATGGTTTGTTTAGATTA+AGG | - | chr8.1:75238191-75238210 | MS.gene060518:CDS | 20.0% |
| !!! | TTTTTCATCTTTTAAAGCTT+TGG | - | chr8.1:75237880-75237899 | MS.gene060518:CDS | 20.0% |
| ! | AATTTATTGGTCAATGATGA+AGG | - | chr8.1:75238238-75238257 | MS.gene060518:CDS | 25.0% |
| ! | TCAACAACGATAATAGATTT+CGG | + | chr8.1:75237653-75237672 | None:intergenic | 25.0% |
| ! | TTAGATTAAGGAATGTTAAG+AGG | - | chr8.1:75238203-75238222 | MS.gene060518:CDS | 25.0% |
| !! | AATGTTGATGGATGATTAAA+CGG | + | chr8.1:75237374-75237393 | None:intergenic | 25.0% |
| AAAGATGAAAAATCACACAC+CGG | + | chr8.1:75237872-75237891 | None:intergenic | 30.0% | |
| AACATCGTTTCAAATGTTGA+TGG | + | chr8.1:75237386-75237405 | None:intergenic | 30.0% | |
| ACTTTACGATAGAACATGTT+CGG | + | chr8.1:75237005-75237024 | None:intergenic | 30.0% | |
| ATAACAAAGAAAATTCGCCA+TGG | + | chr8.1:75237558-75237577 | None:intergenic | 30.0% | |
| ATTATTTCCGGTAATGAGAT+TGG | + | chr8.1:75237075-75237094 | None:intergenic | 30.0% | |
| GAAATCTATTATCGTTGTTG+AGG | - | chr8.1:75237652-75237671 | MS.gene060518:CDS | 30.0% | |
| TAAGGAATGTTAAGAGGAAT+AGG | - | chr8.1:75238209-75238228 | MS.gene060518:CDS | 30.0% | |
| TTTATAGAACCAATAAACCG+TGG | + | chr8.1:75237159-75237178 | None:intergenic | 30.0% | |
| ! | AACATTTGAAACGATGTTGA+TGG | - | chr8.1:75237388-75237407 | MS.gene060518:CDS | 30.0% |
| !!! | GATGTCGTTTTGATTATTTC+CGG | + | chr8.1:75237087-75237106 | None:intergenic | 30.0% |
| AAAACCGAACCAAATCAAAC+AGG | - | chr8.1:75237179-75237198 | MS.gene060518:CDS | 35.0% | |
| AAGTCGGAATCTTCAAAAGA+AGG | + | chr8.1:75237050-75237069 | None:intergenic | 35.0% | |
| AATGTTAAGAGGAATAGGTC+GGG | - | chr8.1:75238214-75238233 | MS.gene060518:CDS | 35.0% | |
| ACAAATGCACCTGTTTGATT+TGG | + | chr8.1:75237191-75237210 | None:intergenic | 35.0% | |
| ACGGATTTGAGGAAGATTTA+TGG | - | chr8.1:75238175-75238194 | MS.gene060518:CDS | 35.0% | |
| ATAAAAGAATCGGTCGTGTT+TGG | - | chr8.1:75237474-75237493 | MS.gene060518:CDS | 35.0% | |
| CGTTTGTTCGTTAATATCAC+CGG | - | chr8.1:75237314-75237333 | MS.gene060518:CDS | 35.0% | |
| GAATAGGTCGGGTAATTTAT+TGG | - | chr8.1:75238225-75238244 | MS.gene060518:CDS | 35.0% | |
| GAATAGTTACTTAGGTGTGA+AGG | - | chr8.1:75237904-75237923 | MS.gene060518:CDS | 35.0% | |
| GAATGTTAAGAGGAATAGGT+CGG | - | chr8.1:75238213-75238232 | MS.gene060518:CDS | 35.0% | |
| TCCGCTGAAATAGAATACAA+CGG | - | chr8.1:75237278-75237297 | MS.gene060518:CDS | 35.0% | |
| TCGGTTTATAACATCGGAAT+TGG | - | chr8.1:75237682-75237701 | MS.gene060518:CDS | 35.0% | |
| ! | AAACGGAGCTTCTTGTTATA+CGG | - | chr8.1:75237497-75237516 | MS.gene060518:CDS | 35.0% |
| ! | ATGGTTTTTCTACGGATTTG+AGG | - | chr8.1:75238164-75238183 | MS.gene060518:CDS | 35.0% |
| ! | GGACGTTCATATTCATTTTC+CGG | - | chr8.1:75237850-75237869 | MS.gene060518:CDS | 35.0% |
| ! | TCTCGATCGGTTTATAACAT+CGG | - | chr8.1:75237676-75237695 | MS.gene060518:CDS | 35.0% |
| ! | TGCAACAAAGCTAGATTTTC+CGG | + | chr8.1:75237534-75237553 | None:intergenic | 35.0% |
| ! | TTCTTGTTATACGGTGAATC+CGG | - | chr8.1:75237506-75237525 | MS.gene060518:CDS | 35.0% |
| AACATCGGAATTGGAATCTC+CGG | - | chr8.1:75237691-75237710 | MS.gene060518:CDS | 40.0% | |
| ATCATCGAAGACAGGTTTCT+CGG | - | chr8.1:75237131-75237150 | MS.gene060518:CDS | 40.0% | |
| CCTGTCTTCGATGATTTGAT+CGG | + | chr8.1:75237126-75237145 | None:intergenic | 40.0% | |
| CGGGCTATTAAATCGGTAAT+CGG | - | chr8.1:75238031-75238050 | MS.gene060518:CDS | 40.0% | |
| GACTTCACCAATCTCATTAC+CGG | - | chr8.1:75237065-75237084 | MS.gene060518:CDS | 40.0% | |
| GCCGTTGTATTCTATTTCAG+CGG | + | chr8.1:75237282-75237301 | None:intergenic | 40.0% | |
| GCTGAGTATTCTGCGTTTAT+CGG | + | chr8.1:75237231-75237250 | None:intergenic | 40.0% | |
| GCTTTGGCGAATAGTTACTT+AGG | - | chr8.1:75237896-75237915 | MS.gene060518:CDS | 40.0% | |
| GGCTATTAAATCGGTAATCG+GGG | - | chr8.1:75238033-75238052 | MS.gene060518:CDS | 40.0% | |
| GGGATTCAGAATTTCATGGA+CGG | - | chr8.1:75237731-75237750 | MS.gene060518:CDS | 40.0% | |
| GGGCTATTAAATCGGTAATC+GGG | - | chr8.1:75238032-75238051 | MS.gene060518:CDS | 40.0% | |
| GGTAATGAGATTGGTGAAGT+CGG | + | chr8.1:75237066-75237085 | None:intergenic | 40.0% | |
| GTATTCTATTTCAGCGGACA+TGG | + | chr8.1:75237276-75237295 | None:intergenic | 40.0% | |
| GTGGAAGATGAAAGTAACCA+AGG | - | chr8.1:75238124-75238143 | MS.gene060518:CDS | 40.0% | |
| TTGTTCGTTAATATCACCGG+CGG | - | chr8.1:75237317-75237336 | MS.gene060518:CDS | 40.0% | |
| ! | AAAGCTAGATTTTCCGGTAC+CGG | + | chr8.1:75237528-75237547 | None:intergenic | 40.0% |
| ! | AGTAACCAAGGTGTTCTTTG+TGG | - | chr8.1:75238136-75238155 | MS.gene060518:CDS | 40.0% |
| !! | AAATGGACGGTGATGGAAAA+GGG | - | chr8.1:75238062-75238081 | MS.gene060518:CDS | 40.0% |
| !! | GAAGGAACACAAGCTTTTTC+CGG | - | chr8.1:75237922-75237941 | MS.gene060518:CDS | 40.0% |
| !! | GTGAGTTGATGATTGCGAAT+CGG | - | chr8.1:75237996-75238015 | MS.gene060518:CDS | 40.0% |
| !! | TGCACCTGTTTGATTTGGTT+CGG | + | chr8.1:75237186-75237205 | None:intergenic | 40.0% |
| !!! | TGGTGGTGATGGTTTTTCTA+CGG | - | chr8.1:75238156-75238175 | MS.gene060518:CDS | 40.0% |
| CCGATCAAATCATCGAAGAC+AGG | - | chr8.1:75237123-75237142 | MS.gene060518:CDS | 45.0% | |
| GAATCGGTCGTGTTTGGAAA+CGG | - | chr8.1:75237480-75237499 | MS.gene060518:CDS | 45.0% | |
| TCGTTGTTGAGGATCTCGAT+CGG | - | chr8.1:75237663-75237682 | MS.gene060518:CDS | 45.0% | |
| TTATACGGTGAATCCGGTAC+CGG | - | chr8.1:75237512-75237531 | MS.gene060518:CDS | 45.0% | |
| ! | AACCAAGGTGTTCTTTGTGG+TGG | - | chr8.1:75238139-75238158 | MS.gene060518:CDS | 45.0% |
| ! | ACCGATTTAATAGCCCGACT+CGG | + | chr8.1:75238028-75238047 | None:intergenic | 45.0% |
| !! | ATCTAGCTTTGTTGCAGCCA+TGG | - | chr8.1:75237538-75237557 | MS.gene060518:CDS | 45.0% |
| !! | CAAATGGACGGTGATGGAAA+AGG | - | chr8.1:75238061-75238080 | MS.gene060518:CDS | 45.0% |
| ATATGAACGTCCACACGACC+AGG | + | chr8.1:75237842-75237861 | None:intergenic | 50.0% | |
| ATCAACTCACCTATCTCCGC+CGG | + | chr8.1:75237986-75238005 | None:intergenic | 50.0% | |
| CACCACCACAAAGAACACCT+TGG | + | chr8.1:75238144-75238163 | None:intergenic | 50.0% | |
| CGACCAGGTCGGAGAAAATT+CGG | + | chr8.1:75237827-75237846 | None:intergenic | 50.0% | |
| GCATTGCAAATGGACGGTGA+TGG | - | chr8.1:75238055-75238074 | MS.gene060518:CDS | 50.0% | |
| GCCGAGTCGGGCTATTAAAT+CGG | - | chr8.1:75238024-75238043 | MS.gene060518:CDS | 50.0% | |
| GGAATTGGAATCTCCGGCAA+CGG | - | chr8.1:75237697-75237716 | MS.gene060518:CDS | 50.0% | |
| GGCGGGGATTCAGAATTTCA+TGG | - | chr8.1:75237727-75237746 | MS.gene060518:CDS | 50.0% | |
| GGTGTGGGGATATGATCGTA+CGG | - | chr8.1:75238083-75238102 | MS.gene060518:CDS | 50.0% | |
| TATGATCGTACGGCGGATAG+AGG | - | chr8.1:75238093-75238112 | MS.gene060518:CDS | 50.0% | |
| TCGTTAATATCACCGGCGGT+GGG | - | chr8.1:75237321-75237340 | MS.gene060518:CDS | 50.0% | |
| TTCGTTAATATCACCGGCGG+TGG | - | chr8.1:75237320-75237339 | MS.gene060518:CDS | 50.0% | |
| ! | GATCCGAATTTTCTCCGACC+TGG | - | chr8.1:75237821-75237840 | MS.gene060518:CDS | 50.0% |
| ! | GGTGTTCTTTGTGGTGGTGA+TGG | - | chr8.1:75238145-75238164 | MS.gene060518:CDS | 50.0% |
| !! | ACGGTGATGGAAAAGGGTGT+GGG | - | chr8.1:75238068-75238087 | MS.gene060518:CDS | 50.0% |
| !! | ATCGGGGCATTGCAAATGGA+CGG | - | chr8.1:75238049-75238068 | MS.gene060518:CDS | 50.0% |
| !! | GGAACACAAGCTTTTTCCGG+CGG | - | chr8.1:75237925-75237944 | MS.gene060518:CDS | 50.0% |
| !! | GGTAATCGGGGCATTGCAAA+TGG | - | chr8.1:75238045-75238064 | MS.gene060518:CDS | 50.0% |
| AGTTTGAGTCCGGCGGAGAT+AGG | - | chr8.1:75237974-75237993 | MS.gene060518:CDS | 55.0% | |
| CGTTAATATCACCGGCGGTG+GGG | - | chr8.1:75237322-75237341 | MS.gene060518:CDS | 55.0% | |
| GGAATCTCCGGCAACGGTTA+CGG | - | chr8.1:75237703-75237722 | MS.gene060518:CDS | 55.0% | |
| GTGGGGATATGATCGTACGG+CGG | - | chr8.1:75238086-75238105 | MS.gene060518:CDS | 55.0% | |
| GTTAATATCACCGGCGGTGG+GGG | - | chr8.1:75237323-75237342 | MS.gene060518:CDS | 55.0% | |
| TTCTCGGCGCCACGGTTTAT+TGG | - | chr8.1:75237147-75237166 | MS.gene060518:CDS | 55.0% | |
| ! | GCGGATAGAGGATGATGACG+TGG | - | chr8.1:75238105-75238124 | MS.gene060518:CDS | 55.0% |
| ! | GTGGAGGAGATTTTCCGGCA+GGG | - | chr8.1:75237947-75237966 | MS.gene060518:CDS | 55.0% |
| ! | TTTTCTCCGACCTGGTCGTG+TGG | - | chr8.1:75237829-75237848 | MS.gene060518:CDS | 55.0% |
| !! | ACACAAGCTTTTTCCGGCGG+TGG | - | chr8.1:75237928-75237947 | MS.gene060518:CDS | 55.0% |
| !! | CGGTGATGGAAAAGGGTGTG+GGG | - | chr8.1:75238069-75238088 | MS.gene060518:CDS | 55.0% |
| !! | GACGGTGATGGAAAAGGGTG+TGG | - | chr8.1:75238067-75238086 | MS.gene060518:CDS | 55.0% |
| AGACAGGTTTCTCGGCGCCA+CGG | - | chr8.1:75237139-75237158 | MS.gene060518:CDS | 60.0% | |
| ATCTCCGGCAACGGTTACGG+CGG | - | chr8.1:75237706-75237725 | MS.gene060518:CDS | 60.0% | |
| CGAATCGGAACTCGCCGAGT+CGG | - | chr8.1:75238011-75238030 | MS.gene060518:CDS | 60.0% | |
| CGGAAAATCTCCTCCACCGC+CGG | + | chr8.1:75237944-75237963 | None:intergenic | 60.0% | |
| GAACGTCCACACGACCAGGT+CGG | + | chr8.1:75237838-75237857 | None:intergenic | 60.0% | |
| GAATCGGAACTCGCCGAGTC+GGG | - | chr8.1:75238012-75238031 | MS.gene060518:CDS | 60.0% | |
| GACTCAAACTCGCTCCCTGC+CGG | + | chr8.1:75237964-75237983 | None:intergenic | 60.0% | |
| GCAGGGAGCGAGTTTGAGTC+CGG | - | chr8.1:75237964-75237983 | MS.gene060518:CDS | 60.0% | |
| ! | CGGCGGTGGAGGAGATTTTC+CGG | - | chr8.1:75237942-75237961 | MS.gene060518:CDS | 60.0% |
| ! | GGTGGAGGAGATTTTCCGGC+AGG | - | chr8.1:75237946-75237965 | MS.gene060518:CDS | 60.0% |
| !! | CAAGCTTTTTCCGGCGGTGG+AGG | - | chr8.1:75237931-75237950 | MS.gene060518:CDS | 60.0% |
| AATATCACCGGCGGTGGGGG+TGG | - | chr8.1:75237326-75237345 | MS.gene060518:CDS | 65.0% | |
| GGGAGCGAGTTTGAGTCCGG+CGG | - | chr8.1:75237967-75237986 | MS.gene060518:CDS | 65.0% | |
| TCCGGCAACGGTTACGGCGG+CGG | - | chr8.1:75237709-75237728 | MS.gene060518:CDS | 70.0% | |
| !! | GGGGTGGTGGAAGGAGGTGG+AGG | - | chr8.1:75237342-75237361 | MS.gene060518:CDS | 70.0% |
| !! | GTGGGGGTGGTGGAAGGAGG+TGG | - | chr8.1:75237339-75237358 | MS.gene060518:CDS | 70.0% |
| ATCACCGGCGGTGGGGGTGG+TGG | - | chr8.1:75237329-75237348 | MS.gene060518:CDS | 75.0% | |
| CCCGCCGCCGTAACCGTTGC+CGG | + | chr8.1:75237713-75237732 | None:intergenic | 75.0% | |
| CCGGCAACGGTTACGGCGGC+GGG | - | chr8.1:75237710-75237729 | MS.gene060518:CDS | 75.0% | |
| CCTTCCACCACCCCCACCGC+CGG | + | chr8.1:75237336-75237355 | None:intergenic | 75.0% | |
| CGGCAACGGTTACGGCGGCG+GGG | - | chr8.1:75237711-75237730 | MS.gene060518:CDS | 75.0% | |
| !! | GCGGTGGGGGTGGTGGAAGG+AGG | - | chr8.1:75237336-75237355 | MS.gene060518:CDS | 75.0% |
| !!! | CCGGCGGTGGGGGTGGTGGA+AGG | - | chr8.1:75237333-75237352 | MS.gene060518:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 75236996 | 75238267 | 75236996 | ID=MS.gene060518 |
| chr8.1 | mRNA | 75236996 | 75238267 | 75236996 | ID=MS.gene060518.t1;Parent=MS.gene060518 |
| chr8.1 | exon | 75236996 | 75238267 | 75236996 | ID=MS.gene060518.t1.exon1;Parent=MS.gene060518.t1 |
| chr8.1 | CDS | 75236996 | 75238267 | 75236996 | ID=cds.MS.gene060518.t1;Parent=MS.gene060518.t1 |
| Gene Sequence |
| Protein sequence |
