AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067314

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067314.t1 MTR_8g024790 94.260 453 24 1 12 462 12 464 0.0 889
MS.gene067314.t1 MTR_8g024770 79.630 432 67 8 38 460 35 454 0.0 682
MS.gene067314.t1 MTR_5g030430 50.990 202 88 4 270 461 474 674 5.80e-55 194
MS.gene067314.t1 MTR_5g030430 32.973 185 91 4 25 180 44 224 3.78e-28 118
MS.gene067314.t1 MTR_8g067280 48.020 202 92 4 271 461 444 643 1.95e-51 184
MS.gene067314.t1 MTR_8g067280 31.282 195 101 6 25 190 53 243 7.45e-25 108
MS.gene067314.t1 MTR_7g096350 28.631 482 241 15 19 440 43 481 2.65e-41 154
MS.gene067314.t1 MTR_7g117670 26.265 514 263 14 25 440 49 544 3.44e-37 144
MS.gene067314.t1 MTR_5g030420 31.720 186 94 4 25 181 27 208 7.50e-29 114
MS.gene067314.t1 MTR_5g030420 31.720 186 94 4 25 181 27 208 9.81e-29 114
MS.gene067314.t1 MTR_8g027495 37.975 158 89 1 283 440 452 600 1.50e-27 116
MS.gene067314.t1 MTR_1g072320 33.526 173 103 3 281 441 457 629 1.24e-18 89.4
MS.gene067314.t1 MTR_1g072320 27.638 199 94 7 19 181 4 188 1.44e-16 82.8
MS.gene067314.t1 MTR_4g097940 30.500 200 111 7 243 426 102 289 6.09e-18 85.1
MS.gene067314.t1 MTR_4g009110 27.363 201 100 5 252 426 133 313 3.48e-17 83.2
MS.gene067314.t1 MTR_2g104490 29.444 180 113 6 272 441 116 291 7.77e-17 81.3
MS.gene067314.t1 MTR_3g104100 33.939 165 98 4 277 431 183 346 1.34e-16 81.6
MS.gene067314.t1 MTR_2g104490 30.625 160 102 5 272 426 116 271 2.22e-16 80.1
MS.gene067314.t1 MTR_0250s0040 28.729 181 109 5 289 457 88 260 3.15e-16 79.0
MS.gene067314.t1 MTR_4g009540 30.435 161 95 5 275 426 142 294 3.55e-16 79.7
MS.gene067314.t1 MTR_4g009540 30.435 161 95 5 275 426 142 294 5.82e-16 79.3
MS.gene067314.t1 MTR_2g104550 25.000 160 114 2 272 426 117 275 5.92e-16 79.0
MS.gene067314.t1 MTR_5g014640 30.899 178 108 5 290 459 155 325 6.72e-16 79.0
MS.gene067314.t1 MTR_6g047570 32.168 143 90 3 260 401 134 270 9.53e-16 78.6
MS.gene067314.t1 MTR_5g014600 28.063 253 148 10 183 418 52 287 2.61e-15 77.4
MS.gene067314.t1 MTR_4g092700 28.161 174 103 4 263 428 137 296 3.53e-15 77.0
MS.gene067314.t1 MTR_1g112680 27.753 227 123 4 241 428 259 483 3.78e-15 78.2
MS.gene067314.t1 MTR_0011s0260 31.159 138 94 1 289 426 183 319 4.01e-15 77.0
MS.gene067314.t1 MTR_4g066460 28.313 166 103 3 262 426 138 288 4.92e-15 76.3
MS.gene067314.t1 MTR_0011s0210 30.216 139 94 2 289 426 183 319 9.55e-15 75.9
MS.gene067314.t1 MTR_2g022280 26.136 176 116 4 288 451 1 174 9.76e-15 73.2
MS.gene067314.t1 MTR_2g081040 35.032 157 87 4 286 430 174 327 1.03e-14 75.9
MS.gene067314.t1 MTR_4g067010 26.857 175 107 5 258 426 121 280 1.08e-14 75.5
MS.gene067314.t1 MTR_4g097920 28.643 199 115 7 243 428 102 286 1.53e-14 75.1
MS.gene067314.t1 MTR_4g097920 28.643 199 115 7 243 428 87 271 1.81e-14 74.3
MS.gene067314.t1 MTR_3g030700 31.792 173 108 4 266 431 173 342 2.01e-14 75.1
MS.gene067314.t1 MTR_8g098275 38.835 103 53 2 288 380 307 409 7.90e-14 73.9
MS.gene067314.t1 MTR_8g098275 38.835 103 53 2 288 380 243 345 8.10e-14 73.6
MS.gene067314.t1 MTR_8g098275 38.835 103 53 2 288 380 400 502 8.59e-14 73.9
MS.gene067314.t1 MTR_7g083900 60.000 55 22 0 287 341 284 338 1.01e-13 73.6
MS.gene067314.t1 MTR_3g498695 58.182 55 23 0 287 341 296 350 1.02e-13 73.6
MS.gene067314.t1 MTR_7g083900 60.000 55 22 0 287 341 284 338 1.02e-13 73.6
MS.gene067314.t1 MTR_3g498695 58.182 55 23 0 287 341 296 350 1.04e-13 73.6
MS.gene067314.t1 MTR_2g010450 30.345 145 85 3 289 425 177 313 1.84e-13 72.0
MS.gene067314.t1 MTR_4g066380 25.728 206 131 6 263 462 137 326 1.94e-13 71.6
MS.gene067314.t1 MTR_6g047550 37.838 111 61 2 260 370 148 250 1.99e-13 70.9
MS.gene067314.t1 MTR_4g097950 30.714 140 87 3 285 418 67 202 4.44e-13 69.3
MS.gene067314.t1 MTR_2g038040 53.226 62 22 1 290 344 53 114 5.11e-13 68.9
MS.gene067314.t1 MTR_0246s0020 49.254 67 30 1 289 355 198 260 8.22e-13 70.1
MS.gene067314.t1 MTR_0246s0020 49.254 67 30 1 289 355 198 260 8.35e-13 70.1
MS.gene067314.t1 MTR_2g104530 28.777 139 94 1 288 426 104 237 9.12e-13 68.9
MS.gene067314.t1 MTR_8g009020 25.316 158 112 2 275 426 129 286 3.77e-12 67.8
MS.gene067314.t1 MTR_4g094762 29.012 162 84 5 290 431 293 443 7.11e-12 67.8
MS.gene067314.t1 MTR_2g104500 25.806 186 130 5 241 426 58 235 2.44e-11 64.7
MS.gene067314.t1 MTR_4g057270 30.000 160 71 7 293 431 153 292 3.76e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067314.t1 AT4G00870 37.527 461 200 14 20 460 29 421 9.81e-73 236
MS.gene067314.t1 AT4G17880 28.864 537 270 13 26 462 63 587 2.94e-68 228
MS.gene067314.t1 AT5G46830 32.952 525 238 17 18 460 18 510 1.06e-64 217
MS.gene067314.t1 AT1G32640 51.980 202 89 4 263 461 427 623 1.49e-56 198
MS.gene067314.t1 AT1G32640 31.579 190 93 3 25 181 67 252 2.40e-30 124
MS.gene067314.t1 AT5G46760 44.770 239 120 5 229 462 359 590 6.90e-54 190
MS.gene067314.t1 AT5G46760 32.065 184 89 5 25 176 51 230 1.81e-26 112
MS.gene067314.t1 AT4G16430 27.215 474 262 13 21 462 45 467 9.20e-41 152
MS.gene067314.t1 AT2G46510 41.714 175 82 3 269 443 376 530 2.22e-34 135
MS.gene067314.t1 AT1G01260 38.889 180 85 3 273 452 417 571 3.17e-30 124
MS.gene067314.t1 AT1G01260 28.571 196 99 5 26 184 48 239 3.74e-13 72.0
MS.gene067314.t1 AT1G01260 38.889 180 85 3 273 452 417 571 3.17e-30 124
MS.gene067314.t1 AT1G01260 28.571 196 99 5 26 184 48 239 3.74e-13 72.0
MS.gene067314.t1 AT1G01260 38.889 180 85 3 273 452 417 571 3.17e-30 124
MS.gene067314.t1 AT1G01260 28.571 196 99 5 26 184 48 239 3.74e-13 72.0
MS.gene067314.t1 AT4G09820 22.998 487 246 16 27 417 17 470 3.01e-24 105
MS.gene067314.t1 AT4G00480 22.201 518 296 15 31 461 26 523 1.43e-19 91.7
MS.gene067314.t1 AT4G00480 22.549 510 294 13 31 456 26 518 2.76e-19 91.3
MS.gene067314.t1 AT4G00480 22.691 498 278 15 31 441 26 503 3.17e-19 90.5
MS.gene067314.t1 AT1G63650 26.486 185 119 3 289 456 405 589 2.90e-16 81.6
MS.gene067314.t1 AT1G63650 26.486 185 119 3 289 456 405 589 2.90e-16 81.6
MS.gene067314.t1 AT1G63650 26.486 185 119 3 289 456 405 589 2.90e-16 81.6
MS.gene067314.t1 AT4G37850 31.325 166 104 5 266 426 130 290 4.01e-16 79.7
MS.gene067314.t1 AT4G37850 31.325 166 104 5 266 426 130 290 4.01e-16 79.7
MS.gene067314.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.72e-14 75.9
MS.gene067314.t1 AT1G12860 57.895 57 24 0 287 343 265 321 1.78e-14 75.5
MS.gene067314.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.78e-14 75.9
MS.gene067314.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.78e-14 75.9
MS.gene067314.t1 AT3G26744 60.000 55 22 0 287 341 305 359 1.78e-14 75.9
MS.gene067314.t1 AT1G12860 57.895 57 24 0 287 343 265 321 2.11e-14 75.5
MS.gene067314.t1 AT5G57150 26.908 249 138 10 233 461 7 231 7.68e-14 71.6
MS.gene067314.t1 AT5G57150 27.193 228 128 8 233 441 7 215 1.34e-13 70.5
MS.gene067314.t1 AT5G57150 27.468 233 126 10 233 443 7 218 1.71e-13 70.9
MS.gene067314.t1 AT3G06120 27.869 183 111 4 288 449 1 183 1.78e-13 69.7
MS.gene067314.t1 AT5G57150 26.754 228 129 8 233 441 7 215 2.14e-13 69.7
MS.gene067314.t1 AT4G29930 54.098 61 21 1 290 343 55 115 2.26e-13 70.1
MS.gene067314.t1 AT4G29930 54.098 61 21 1 290 343 55 115 2.60e-13 69.7
MS.gene067314.t1 AT4G29930 54.098 61 21 1 290 343 55 115 2.81e-13 69.7
MS.gene067314.t1 AT5G57150 36.719 128 59 5 233 359 7 113 2.99e-13 69.3
MS.gene067314.t1 AT4G29930 55.000 60 20 1 291 343 56 115 4.22e-13 68.2
MS.gene067314.t1 AT4G29930 54.098 61 21 1 290 343 55 115 4.76e-13 69.7
MS.gene067314.t1 AT4G29930 54.098 61 21 1 290 343 55 115 4.76e-13 69.7
MS.gene067314.t1 AT4G29930 54.098 61 21 1 290 343 55 115 5.24e-13 69.3
MS.gene067314.t1 AT1G10610 32.847 137 85 4 290 421 235 369 1.89e-12 69.3
MS.gene067314.t1 AT1G10610 32.847 137 85 4 290 421 265 399 2.22e-12 69.3
MS.gene067314.t1 AT1G10610 32.847 137 85 4 290 421 168 302 2.34e-12 68.6
MS.gene067314.t1 AT5G41315 32.258 93 49 1 289 381 449 527 2.77e-12 69.3
MS.gene067314.t1 AT5G41315 32.258 93 49 1 289 381 446 524 2.81e-12 69.3
MS.gene067314.t1 AT5G41315 32.258 93 49 1 289 381 441 519 2.87e-12 69.3
MS.gene067314.t1 AT2G22760 28.415 183 106 7 276 439 100 276 6.83e-12 66.6
MS.gene067314.t1 AT2G16910 54.000 50 23 0 293 342 318 367 1.13e-11 67.4
MS.gene067314.t1 AT3G24140 23.721 215 137 4 269 456 175 389 1.63e-11 66.2
MS.gene067314.t1 AT2G22760 29.697 165 96 6 276 426 100 258 1.91e-11 65.1
MS.gene067314.t1 AT3G24140 23.721 215 137 4 269 456 175 389 2.36e-11 65.9

Find 77 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCATTTGTTGCAAATTT+TGG 0.152440 8.4:-70356233 None:intergenic
TCCAAACCCAATCCGAAAAT+TGG 0.161848 8.4:+70355647 MS.gene067314:CDS
ATTGAAGGATTTGGAGTTTC+AGG 0.165432 8.4:+70356785 MS.gene067314:CDS
GAGATTGATGTGAAGATAAT+AGG 0.216324 8.4:+70356696 MS.gene067314:CDS
AAGGTAAACCTTTCTTATCT+TGG 0.238310 8.4:+70355707 MS.gene067314:CDS
GTTTGAAATTGCTGTTTCTT+TGG 0.265579 8.4:-70355752 None:intergenic
ATGACAATATCCAACAAAAT+TGG 0.270722 8.4:+70356037 MS.gene067314:CDS
TTATCCATCTTTGAAACATT+TGG 0.290544 8.4:-70356483 None:intergenic
AGGTAAACCTTTCTTATCTT+GGG 0.292720 8.4:+70355708 MS.gene067314:CDS
ATCTTAAAGCATAGAATCTA+TGG 0.304209 8.4:-70356452 None:intergenic
TTGCTGTTTCTTTGGTTCCT+TGG 0.308281 8.4:-70355744 None:intergenic
AAGATTGAAGATTTAGAATC+AGG 0.311502 8.4:+70356555 MS.gene067314:CDS
AATGAGTGTATTGAAGGATT+TGG 0.327266 8.4:+70356776 MS.gene067314:CDS
TACCGATGTCGGCGAATGTT+TGG 0.330440 8.4:-70356161 None:intergenic
ATTCTTGTTGGTGTTGCTAA+TGG 0.335278 8.4:-70356312 None:intergenic
TCCATGCACGTGTGCTTCAT+TGG 0.351119 8.4:-70355959 None:intergenic
AGGGTTAGGGTTTGTGGTGA+AGG 0.352816 8.4:-70355601 None:intergenic
TGTTGAAGGGTTAGGGTTTG+TGG 0.360355 8.4:-70355607 None:intergenic
AACTAACGGTGTAATCGAAA+TGG 0.368563 8.4:+70356008 MS.gene067314:CDS
ATCAGTGTCTTCTCCGGAAC+CGG 0.379047 8.4:-70356190 None:intergenic
AGAGGAAGAAAACCGTTATC+AGG 0.381422 8.4:+70356372 MS.gene067314:CDS
TCAGCCATTCTCATGAAATT+AGG 0.386390 8.4:+70356906 MS.gene067314:CDS
TAGATTCTGATGTGGATGTT+TGG 0.396023 8.4:-70356089 None:intergenic
ATCGACTGTTAATGAAGTTA+TGG 0.398979 8.4:+70356824 MS.gene067314:CDS
TAGATTTGGCTTGGTGTACT+AGG 0.400415 8.4:-70356062 None:intergenic
CAAACAACAAAAGATGAAAA+AGG 0.404302 8.4:+70355688 MS.gene067314:CDS
ATCGGTATCGTCTCCGGTTC+CGG 0.406820 8.4:+70356177 MS.gene067314:CDS
GGATGTTTGGAACATAGATT+TGG 0.408652 8.4:-70356076 None:intergenic
TCTGCTTCCACATGGTTTAA+CGG 0.415651 8.4:-70356405 None:intergenic
AAGATTAATGAGTGTATTGA+AGG 0.416800 8.4:+70356770 MS.gene067314:CDS
GGGTTAGGGTTTGTGGTGAA+GGG 0.421882 8.4:-70355600 None:intergenic
TTGCTTTAGATTCTGTACTT+TGG 0.425852 8.4:+70355893 MS.gene067314:CDS
AGATTTGTCAAGTGGGTTTG+TGG 0.429194 8.4:-70356133 None:intergenic
GGTTCCAAATGTTTCAAAGA+TGG 0.435449 8.4:+70356479 MS.gene067314:CDS
AAAAGTGAAGATGGAAATGA+TGG 0.445716 8.4:+70356596 MS.gene067314:CDS
CCTTTCTTATCTTGGGGTGA+AGG 0.446476 8.4:+70355715 MS.gene067314:CDS
TCTTCCGCGCTCATCTCGTT+AGG 0.463829 8.4:-70356870 None:intergenic
CCAAACCCAATCCGAAAATT+GGG 0.468035 8.4:+70355648 MS.gene067314:CDS
TGTCTCATCAGTGTCTTCTC+CGG 0.468594 8.4:-70356196 None:intergenic
GACAGCAACACGTGTACAAA+TGG 0.479858 8.4:+70355784 MS.gene067314:CDS
CTGACCTAATTTCATGAGAA+TGG 0.482002 8.4:-70356910 None:intergenic
GCTTCTTCAATTTCATTACC+TGG 0.483921 8.4:+70355862 MS.gene067314:CDS
ACTAACGGTGTAATCGAAAT+GGG 0.485666 8.4:+70356009 MS.gene067314:CDS
CCTTCACCCCAAGATAAGAA+AGG 0.497048 8.4:-70355715 None:intergenic
TCGATTACACCGTTAGTTGT+CGG 0.498052 8.4:-70356003 None:intergenic
ATTTGTATTCCGACAACTAA+CGG 0.507792 8.4:+70355994 MS.gene067314:CDS
GGTGTTTGAGTTCCTGATAA+CGG 0.508977 8.4:-70356384 None:intergenic
GCCGACATCGGTATCGTCTC+CGG 0.512586 8.4:+70356171 MS.gene067314:CDS
TTTAGAATCAGGGCAACAAA+AGG 0.514365 8.4:+70356566 MS.gene067314:CDS
TTTGTCTCTCTGCTTCCACA+TGG 0.518910 8.4:-70356413 None:intergenic
TCCAATGAAGCACACGTGCA+TGG 0.524272 8.4:+70355958 MS.gene067314:CDS
TGGAACATAGATTTGTCAAG+TGG 0.527748 8.4:-70356141 None:intergenic
ATTCTATGCTTTAAGATCTG+TGG 0.527981 8.4:+70356458 MS.gene067314:CDS
ACACTCATTAATCTTGCACT+AGG 0.528247 8.4:-70356762 None:intergenic
AATTGAAGAAGCATTACCTA+TGG 0.532558 8.4:-70355851 None:intergenic
TTTGGAACATAGATTTGGCT+TGG 0.537383 8.4:-70356071 None:intergenic
GGTAAACCTTTCTTATCTTG+GGG 0.537701 8.4:+70355709 MS.gene067314:CDS
TTCCAAACATTCGCCGACAT+CGG 0.537863 8.4:+70356159 MS.gene067314:CDS
ATGTGGAAGCAGAGAGACAA+AGG 0.547229 8.4:+70356415 MS.gene067314:CDS
ATCATAGAGGAAGATGAAGA+AGG 0.553296 8.4:-70355553 None:intergenic
TCAAAAGCATTAACATTACA+AGG 0.560113 8.4:-70356675 None:intergenic
AGATTGAAGATTTAGAATCA+GGG 0.563283 8.4:+70356556 MS.gene067314:CDS
CTTGTTGGTGTTGCTAATGG+TGG 0.567083 8.4:-70356309 None:intergenic
GATAATAGGAAATGATGCTA+TGG 0.577854 8.4:+70356710 MS.gene067314:CDS
TGCAACGGCAACTTCAACTG+TGG 0.578390 8.4:+70356638 MS.gene067314:CDS
TGGGGTGAAGGCTATTTCCA+AGG 0.580662 8.4:+70355727 MS.gene067314:CDS
GGAACATAGATTTGTCAAGT+GGG 0.591339 8.4:-70356140 None:intergenic
GTACAAATGGTGATGCTGAA+TGG 0.602234 8.4:+70355797 MS.gene067314:CDS
TCTAAAGCAAAAGCTTTACC+AGG 0.602957 8.4:-70355880 None:intergenic
AAAAGAGAACCTAAGAAGAG+AGG 0.611347 8.4:+70356354 MS.gene067314:CDS
TTCTGAGTTAGATTCTGATG+TGG 0.614919 8.4:-70356097 None:intergenic
TGTCGATAATAACAGTGCAA+CGG 0.631393 8.4:+70356623 MS.gene067314:CDS
CACTCATTAATCTTGCACTA+GGG 0.639299 8.4:-70356761 None:intergenic
ATGAGGAAATGATATCATAG+AGG 0.640466 8.4:-70355566 None:intergenic
ACCGGAGACGATACCGATGT+CGG 0.645053 8.4:-70356172 None:intergenic
TCAAACACCGTTAAACCATG+TGG 0.685029 8.4:+70356398 MS.gene067314:CDS
AGTTCCTAACGAGATGAGCG+CGG 0.706023 8.4:+70356866 MS.gene067314:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTGAAGTTTTTGTTGA+AGG - chr8.4:70355624-70355643 None:intergenic 20.0%
!!! TAATTGAAGTTTTTGTTGAA+GGG - chr8.4:70355623-70355642 None:intergenic 20.0%
!!! TATTCATTTGTTGCAAATTT+TGG - chr8.4:70356236-70356255 None:intergenic 20.0%
! AAGATTAATGAGTGTATTGA+AGG + chr8.4:70356770-70356789 MS.gene067314:CDS 25.0%
! ATCTTAAAGCATAGAATCTA+TGG - chr8.4:70356455-70356474 None:intergenic 25.0%
! ATGACAATATCCAACAAAAT+TGG + chr8.4:70356037-70356056 MS.gene067314:CDS 25.0%
! CAAACAACAAAAGATGAAAA+AGG + chr8.4:70355688-70355707 MS.gene067314:CDS 25.0%
!! AAGATTGAAGATTTAGAATC+AGG + chr8.4:70356555-70356574 MS.gene067314:CDS 25.0%
!! AGATTGAAGATTTAGAATCA+GGG + chr8.4:70356556-70356575 MS.gene067314:CDS 25.0%
!!! GAATCTTTTTCAATTCTTGT+TGG - chr8.4:70356327-70356346 None:intergenic 25.0%
!!! TAACATTACAAGGTCTTTTT+TGG - chr8.4:70356668-70356687 None:intergenic 25.0%
!!! TCAAAAGCATTAACATTACA+AGG - chr8.4:70356678-70356697 None:intergenic 25.0%
!!! TTATCCATCTTTGAAACATT+TGG - chr8.4:70356486-70356505 None:intergenic 25.0%
AAAAGTGAAGATGGAAATGA+TGG + chr8.4:70356596-70356615 MS.gene067314:CDS 30.0%
AAGGTAAACCTTTCTTATCT+TGG + chr8.4:70355707-70355726 MS.gene067314:CDS 30.0%
AATGAGTGTATTGAAGGATT+TGG + chr8.4:70356776-70356795 MS.gene067314:CDS 30.0%
AGGTAAACCTTTCTTATCTT+GGG + chr8.4:70355708-70355727 MS.gene067314:CDS 30.0%
ATCGACTGTTAATGAAGTTA+TGG + chr8.4:70356824-70356843 MS.gene067314:CDS 30.0%
ATGAGGAAATGATATCATAG+AGG - chr8.4:70355569-70355588 None:intergenic 30.0%
ATTTGTATTCCGACAACTAA+CGG + chr8.4:70355994-70356013 MS.gene067314:CDS 30.0%
GAGATTGATGTGAAGATAAT+AGG + chr8.4:70356696-70356715 MS.gene067314:CDS 30.0%
GATAATAGGAAATGATGCTA+TGG + chr8.4:70356710-70356729 MS.gene067314:CDS 30.0%
! AAGTTTTTGTTGAAGGGTTA+GGG - chr8.4:70355617-70355636 None:intergenic 30.0%
! TTGAGTCGAAATTTTTCCAT+AGG + chr8.4:70355835-70355854 MS.gene067314:CDS 30.0%
! TTGCTTTAGATTCTGTACTT+TGG + chr8.4:70355893-70355912 MS.gene067314:CDS 30.0%
!! AATTGAAGAAGCATTACCTA+TGG - chr8.4:70355854-70355873 None:intergenic 30.0%
!! ATTCTATGCTTTAAGATCTG+TGG + chr8.4:70356458-70356477 MS.gene067314:CDS 30.0%
!! GTTTGAAATTGCTGTTTCTT+TGG - chr8.4:70355755-70355774 None:intergenic 30.0%
!!! TTTTGTTGTTTGCCACAAAA+TGG - chr8.4:70355680-70355699 None:intergenic 30.0%
AAAAGAGAACCTAAGAAGAG+AGG + chr8.4:70356354-70356373 MS.gene067314:CDS 35.0%
AACTAACGGTGTAATCGAAA+TGG + chr8.4:70356008-70356027 MS.gene067314:CDS 35.0%
ACACTCATTAATCTTGCACT+AGG - chr8.4:70356765-70356784 None:intergenic 35.0%
ACTAACGGTGTAATCGAAAT+GGG + chr8.4:70356009-70356028 MS.gene067314:CDS 35.0%
ATCATAGAGGAAGATGAAGA+AGG - chr8.4:70355556-70355575 None:intergenic 35.0%
CACTCATTAATCTTGCACTA+GGG - chr8.4:70356764-70356783 None:intergenic 35.0%
CTGACCTAATTTCATGAGAA+TGG - chr8.4:70356913-70356932 None:intergenic 35.0%
GCTTCTTCAATTTCATTACC+TGG + chr8.4:70355862-70355881 MS.gene067314:CDS 35.0%
GGAACATAGATTTGTCAAGT+GGG - chr8.4:70356143-70356162 None:intergenic 35.0%
GGAATCGAAAAAAGTGAAGA+TGG + chr8.4:70356587-70356606 MS.gene067314:CDS 35.0%
GGTAAACCTTTCTTATCTTG+GGG + chr8.4:70355709-70355728 MS.gene067314:CDS 35.0%
GGTTCCAAATGTTTCAAAGA+TGG + chr8.4:70356479-70356498 MS.gene067314:CDS 35.0%
TAGATTCTGATGTGGATGTT+TGG - chr8.4:70356092-70356111 None:intergenic 35.0%
TCAGCCATTCTCATGAAATT+AGG + chr8.4:70356906-70356925 MS.gene067314:CDS 35.0%
TGGAACATAGATTTGTCAAG+TGG - chr8.4:70356144-70356163 None:intergenic 35.0%
TGTCGATAATAACAGTGCAA+CGG + chr8.4:70356623-70356642 MS.gene067314:CDS 35.0%
TTCTGAGTTAGATTCTGATG+TGG - chr8.4:70356100-70356119 None:intergenic 35.0%
! AATGGCATAGACCCAATTTT+CGG - chr8.4:70355662-70355681 None:intergenic 35.0%
! ATAGACCCAATTTTCGGATT+GGG - chr8.4:70355656-70355675 None:intergenic 35.0%
! ATTGAAGGATTTGGAGTTTC+AGG + chr8.4:70356785-70356804 MS.gene067314:CDS 35.0%
! GAAGGGTTTTCATTTTCATG+AGG - chr8.4:70355586-70355605 None:intergenic 35.0%
! GAAGTTTTTGTTGAAGGGTT+AGG - chr8.4:70355618-70355637 None:intergenic 35.0%
! GGATGTTTGGAACATAGATT+TGG - chr8.4:70356079-70356098 None:intergenic 35.0%
! GTACTAGGTTCCAATTTTGT+TGG - chr8.4:70356050-70356069 None:intergenic 35.0%
! TCTAAAGCAAAAGCTTTACC+AGG - chr8.4:70355883-70355902 None:intergenic 35.0%
! TTCTCTTTTATCAGATGCAG+TGG + chr8.4:70356509-70356528 MS.gene067314:CDS 35.0%
! TTTAGAATCAGGGCAACAAA+AGG + chr8.4:70356566-70356585 MS.gene067314:CDS 35.0%
! TTTGGAACATAGATTTGGCT+TGG - chr8.4:70356074-70356093 None:intergenic 35.0%
!! ATTCTTGTTGGTGTTGCTAA+TGG - chr8.4:70356315-70356334 None:intergenic 35.0%
AGAGGAAGAAAACCGTTATC+AGG + chr8.4:70356372-70356391 MS.gene067314:CDS 40.0%
CCAAACCCAATCCGAAAATT+GGG + chr8.4:70355648-70355667 MS.gene067314:CDS 40.0%
TCAAACACCGTTAAACCATG+TGG + chr8.4:70356398-70356417 MS.gene067314:CDS 40.0%
TCCAAACCCAATCCGAAAAT+TGG + chr8.4:70355647-70355666 MS.gene067314:CDS 40.0%
TCGATTACACCGTTAGTTGT+CGG - chr8.4:70356006-70356025 None:intergenic 40.0%
TCTGCTTCCACATGGTTTAA+CGG - chr8.4:70356408-70356427 None:intergenic 40.0%
! AGATTTGTCAAGTGGGTTTG+TGG - chr8.4:70356136-70356155 None:intergenic 40.0%
! CATAGACCCAATTTTCGGAT+TGG - chr8.4:70355657-70355676 None:intergenic 40.0%
! GGTGTTTGAGTTCCTGATAA+CGG - chr8.4:70356387-70356406 None:intergenic 40.0%
! GGTTTTCTTCCTCTCTTCTT+AGG - chr8.4:70356366-70356385 None:intergenic 40.0%
! GTACAAATGGTGATGCTGAA+TGG + chr8.4:70355797-70355816 MS.gene067314:CDS 40.0%
!! TAGATTTGGCTTGGTGTACT+AGG - chr8.4:70356065-70356084 None:intergenic 40.0%
!! TTGCTGTTTCTTTGGTTCCT+TGG - chr8.4:70355747-70355766 None:intergenic 40.0%
!!! ATTGGGTCTATGCCATTTTG+TGG + chr8.4:70355665-70355684 MS.gene067314:CDS 40.0%
ATGTGGAAGCAGAGAGACAA+AGG + chr8.4:70356415-70356434 MS.gene067314:CDS 45.0%
CCTTCACCCCAAGATAAGAA+AGG - chr8.4:70355718-70355737 None:intergenic 45.0%
CCTTTCTTATCTTGGGGTGA+AGG + chr8.4:70355715-70355734 MS.gene067314:CDS 45.0%
GACAGCAACACGTGTACAAA+TGG + chr8.4:70355784-70355803 MS.gene067314:CDS 45.0%
TGTCTCATCAGTGTCTTCTC+CGG - chr8.4:70356199-70356218 None:intergenic 45.0%
TGTTGAAGGGTTAGGGTTTG+TGG - chr8.4:70355610-70355629 None:intergenic 45.0%
TTCCAAACATTCGCCGACAT+CGG + chr8.4:70356159-70356178 MS.gene067314:CDS 45.0%
TTTGTCTCTCTGCTTCCACA+TGG - chr8.4:70356416-70356435 None:intergenic 45.0%
!! CCCAATTTTCGGATTGGGTT+TGG - chr8.4:70355651-70355670 None:intergenic 45.0%
!! CTTGTTGGTGTTGCTAATGG+TGG - chr8.4:70356312-70356331 None:intergenic 45.0%
AGGGTTAGGGTTTGTGGTGA+AGG - chr8.4:70355604-70355623 None:intergenic 50.0%
AGTTCCTAACGAGATGAGCG+CGG + chr8.4:70356866-70356885 MS.gene067314:CDS 50.0%
ATCAGTGTCTTCTCCGGAAC+CGG - chr8.4:70356193-70356212 None:intergenic 50.0%
GGGTTAGGGTTTGTGGTGAA+GGG - chr8.4:70355603-70355622 None:intergenic 50.0%
TACCGATGTCGGCGAATGTT+TGG - chr8.4:70356164-70356183 None:intergenic 50.0%
TCCAATGAAGCACACGTGCA+TGG + chr8.4:70355958-70355977 MS.gene067314:CDS 50.0%
TCCATGCACGTGTGCTTCAT+TGG - chr8.4:70355962-70355981 None:intergenic 50.0%
TGCAACGGCAACTTCAACTG+TGG + chr8.4:70356638-70356657 MS.gene067314:CDS 50.0%
! TGGGGTGAAGGCTATTTCCA+AGG + chr8.4:70355727-70355746 MS.gene067314:CDS 50.0%
ACCGGAGACGATACCGATGT+CGG - chr8.4:70356175-70356194 None:intergenic 55.0%
ATCGGTATCGTCTCCGGTTC+CGG + chr8.4:70356177-70356196 MS.gene067314:CDS 55.0%
TCTTCCGCGCTCATCTCGTT+AGG - chr8.4:70356873-70356892 None:intergenic 55.0%
GCCGACATCGGTATCGTCTC+CGG + chr8.4:70356171-70356190 MS.gene067314:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 70355547 70356935 70355547 ID=MS.gene067314
chr8.4 mRNA 70355547 70356935 70355547 ID=MS.gene067314.t1;Parent=MS.gene067314
chr8.4 exon 70355547 70356935 70355547 ID=MS.gene067314.t1.exon1;Parent=MS.gene067314.t1
chr8.4 CDS 70355547 70356935 70355547 ID=cds.MS.gene067314.t1;Parent=MS.gene067314.t1
Gene Sequence

>MS.gene067314

ATGTCACCTTCTTCATCTTCCTCTATGATATCATTTCCTCATGAAAATGAAAACCCTTCACCACAAACCCTAACCCTTCAACAAAAACTTCAATTATTAGTCCAAACCCAATCCGAAAATTGGGTCTATGCCATTTTGTGGCAAACAACAAAAGATGAAAAAGGTAAACCTTTCTTATCTTGGGGTGAAGGCTATTTCCAAGGAACCAAAGAAACAGCAATTTCAAACAAACAACACGACAGCAACACGTGTACAAATGGTGATGCTGAATGGTTCTATGTGATGTCGTTGAGTCGAAATTTTTCCATAGGTAATGCTTCTTCAATTTCATTACCTGGTAAAGCTTTTGCTTTAGATTCTGTACTTTGGTTGAATAGTAAACATGAACTTCAAATCTATAACTGTGAGAGATCCAATGAAGCACACGTGCATGGAATTGAAACATTGATTTGTATTCCGACAACTAACGGTGTAATCGAAATGGGTTCTTATGACAATATCCAACAAAATTGGAACCTAGTACACCAAGCCAAATCTATGTTCCAAACATCCACATCAGAATCTAACTCAGAAATAGATCTCATCTCCACAAACCCACTTGACAAATCTATGTTCCAAACATTCGCCGACATCGGTATCGTCTCCGGTTCCGGAGAAGACACTGATGAGACACAAAAAACAATGAACCAAAATTTGCAACAAATGAATAACATTGTTTCATCATGTTCTTTTGATTCTGAAAATTCTGATTCAGAATATTGTCCACCATTAGCAACACCAACAAGAATTGAAAAAGATTCTTTTGAGAAAAGAGAACCTAAGAAGAGAGGAAGAAAACCGTTATCAGGAACTCAAACACCGTTAAACCATGTGGAAGCAGAGAGACAAAGGAGAGAGAAACTAAACCATAGATTCTATGCTTTAAGATCTGTGGTTCCAAATGTTTCAAAGATGGATAAAGCTTCTCTTTTATCAGATGCAGTGGATTACATCAACGAGTTAAAAGCAAAGATTGAAGATTTAGAATCAGGGCAACAAAAGGAATCGAAAAAAGTGAAGATGGAAATGATGGAAATTGTCGATAATAACAGTGCAACGGCAACTTCAACTGTGGTAGACCAAAAAAGACCTTGTAATGTTAATGCTTTTGAGATTGATGTGAAGATAATAGGAAATGATGCTATGGTGAGAGTTCAAAGTGAGAATGTTAATCACCCTAGTGCAAGATTAATGAGTGTATTGAAGGATTTGGAGTTTCAGGTACATCATGCTAGTATATCGACTGTTAATGAAGTTATGGTTCAAGATGTTGTTGTTCGAGTTCCTAACGAGATGAGCGCGGAAGAGAGTCTAATTAGATCAGCCATTCTCATGAAATTAGGTCAGTAG
Protein sequence

>MS.gene067314.t1

MSPSSSSSMISFPHENENPSPQTLTLQQKLQLLVQTQSENWVYAILWQTTKDEKGKPFLSWGEGYFQGTKETAISNKQHDSNTCTNGDAEWFYVMSLSRNFSIGNASSISLPGKAFALDSVLWLNSKHELQIYNCERSNEAHVHGIETLICIPTTNGVIEMGSYDNIQQNWNLVHQAKSMFQTSTSESNSEIDLISTNPLDKSMFQTFADIGIVSGSGEDTDETQKTMNQNLQQMNNIVSSCSFDSENSDSEYCPPLATPTRIEKDSFEKREPKKRGRKPLSGTQTPLNHVEAERQRREKLNHRFYALRSVVPNVSKMDKASLLSDAVDYINELKAKIEDLESGQQKESKKVKMEMMEIVDNNSATATSTVVDQKRPCNVNAFEIDVKIIGNDAMVRVQSENVNHPSARLMSVLKDLEFQVHHASISTVNEVMVQDVVVRVPNEMSAEESLIRSAILMKLGQ